Rroxscaffold_7G00217240

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0009691
Physical Location & Seq
Reverse (-)
67732801 .. 67733709
909 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00217240.1

Sequence Viewer

Length: 447 bp
ATGAGGTTGACAAGCTCTGCTGTCGTCTTACGGCTAACACCCAACGCCTGCCTCAAGTATCTCATCATCCATGATGTAGCCAATGTGCAACAGCAAGTGCATGTTCGCATCTGCCGTCAACCAAGGCCGTCTTCGGTTCGGTCGGTCCTGGAGGGCTTGCATTGTTTTGGGAGGACGGCCGGGATGTTCGATTCCGGTCCAAGTCGGCGTATCATGTGGATGTGGGAAGTGTACCCCTCTAATGGTTCGATGTTGATCCGGAGGTTGATTGGGTTTATGGACACCGACCACGGCGATGCGTCACATTACCTCGGTCGTCGCTTCATCAACCGTGGGAGGAGACGTCGTCGCCCATGGGTGGTCCTAGGAGACTTCAATGAGTTGCTCCATGCGGAGAGAAGTCTAGTGGGTGTACCTCGGGAGGGGAATCGCAAATGCAACTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

148

Amino Acids

17.1

Weight (kDa)

11.25

Isoelectric Point (pI)

64.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 346
AccIII TCCGGA 1 cut(s) 258
AciI CCGC 1 cut(s) 392
AclWI GGATC 1 cut(s) 250
AcoI YGGCCR 1 cut(s) 177
AcyI GRCGYC 1 cut(s) 343
AfaI GTAC 2 cut(s) 233, 414
AfiI CCNNNNNNNGG 3 cut(s) 242, 358, 422
AgsI TTSAA 1 cut(s) 376
AjnI CCWGG 1 cut(s) 147
AjuI GAANNNNNNNTTGG 2 cut(s) 115, 147
AluBI AGCT 1 cut(s) 15
AluI AGCT 1 cut(s) 15
Alw26I GTCTC 2 cut(s) 334, 363
AlwI GGATC 1 cut(s) 250
Ama87I CYCGRG 1 cut(s) 417
Aor13HI TCCGGA 1 cut(s) 258
AoxI GGCC 2 cut(s) 125, 177
AspA2I CCTAGG 1 cut(s) 364
AspS9I GGNCC 3 cut(s) 145, 197, 361
AsuC2I CCSGG 1 cut(s) 181
AvaI CYCGRG 1 cut(s) 417
AvaII GGWCC 3 cut(s) 145, 197, 361
AvrII CCTAGG 1 cut(s) 364
BbsI GAAGAC 1 cut(s) 123
BceAI ACGGC 5 cut(s) 47, 99, 112, 192, 307
BciT130I CCWGG 1 cut(s) 149
BcnI CCSGG 1 cut(s) 181
BcoDI GTCTC 2 cut(s) 334, 363
BfaI CTAG 2 cut(s) 365, 404
BlnI CCTAGG 1 cut(s) 364
Bme1390I CCNGG 2 cut(s) 149, 181
Bme18I GGWCC 3 cut(s) 145, 197, 361
BmeT110I CYCGRG 1 cut(s) 417
BmgT120I GGNCC 3 cut(s) 145, 197, 361
BmrFI CCNGG 2 cut(s) 149, 181
BmsI GCATC 2 cut(s) 117, 286
BpiI GAAGAC 1 cut(s) 123
BpmI CTGGAG 1 cut(s) 170
BpuEI CTTGAG 1 cut(s) 38
BpuMI CCSGG 1 cut(s) 181
BsaBI GATNNNNATC 1 cut(s) 254
BsaHI GRCGYC 1 cut(s) 343
BsaJI CCNNGG 7 cut(s) 122, 289, 310, 331, 353, 364, 416
BsaWI WCCGGW 2 cut(s) 194, 258
BsaXI ACNNNNNCTCC 4 cut(s) 328, 331, 358, 361
Bsc4I CCNNNNNNNGG 3 cut(s) 242, 358, 422
Bse8I GATNNNNATC 1 cut(s) 254
BseAI TCCGGA 1 cut(s) 258
BseBI CCWGG 1 cut(s) 149
BseDI CCNNGG 7 cut(s) 122, 289, 310, 331, 353, 364, 416
BseGI GGATG 3 cut(s) 66, 189, 225
BseJI GATNNNNATC 1 cut(s) 254
BseLI CCNNNNNNNGG 3 cut(s) 242, 358, 422
BseRI GAGGAG 1 cut(s) 352
BseX3I CGGCCG 1 cut(s) 177
Bsh1285I CGRYCG 3 cut(s) 143, 180, 316
BshFI GGCC 2 cut(s) 127, 179
BsiEI CGRYCG 3 cut(s) 143, 180, 316
BsiHKCI CYCGRG 1 cut(s) 417
BsiSI CCGG 3 cut(s) 180, 195, 259
BslI CCNNNNNNNGG 3 cut(s) 242, 358, 422
BsmAI GTCTC 2 cut(s) 334, 363
BsmBI CGTCTC 1 cut(s) 334
BsnI GGCC 2 cut(s) 127, 179
BsoBI CYCGRG 1 cut(s) 417
Bsp13I TCCGGA 1 cut(s) 258
Bsp143I GATC 1 cut(s) 255
Bsp19I CCATGG 1 cut(s) 353
BspACI CCGC 1 cut(s) 392
BspANI GGCC 2 cut(s) 127, 179
BspEI TCCGGA 1 cut(s) 258
BspPI GGATC 1 cut(s) 250
BssECI CCNNGG 7 cut(s) 122, 289, 310, 331, 353, 364, 416
BssMI GATC 1 cut(s) 255
BssNI GRCGYC 1 cut(s) 343
BssT1I CCWWGG 3 cut(s) 122, 353, 364
Bst2UI CCWGG 1 cut(s) 149
Bst4CI ACNGT 1 cut(s) 332
BstACI GRCGYC 1 cut(s) 343
BstC8I GCNNGC 2 cut(s) 49, 158
BstDSI CCRYGG 3 cut(s) 289, 331, 353
BstF5I GGATG 3 cut(s) 66, 189, 225
BstKTI GATC 1 cut(s) 258
BstMAI GTCTC 2 cut(s) 334, 363
BstMBI GATC 1 cut(s) 255
BstMCI CGRYCG 3 cut(s) 143, 180, 316
BstNI CCWGG 1 cut(s) 149
BstNSI RCATGY 1 cut(s) 104
BstSCI CCNGG 2 cut(s) 147, 179
BstV2I GAAGAC 1 cut(s) 123
BstZI CGGCCG 1 cut(s) 177
BsuRI GGCC 2 cut(s) 127, 179
BtgI CCRYGG 3 cut(s) 289, 331, 353
BtgZI GCGATG 1 cut(s) 309
BtsCI GGATG 3 cut(s) 66, 189, 225
Cac8I GCNNGC 2 cut(s) 49, 158
Cfr13I GGNCC 3 cut(s) 145, 197, 361
CseI GACGC 1 cut(s) 288
Csp6I GTAC 2 cut(s) 232, 413
CviAII CATG 5 cut(s) 71, 101, 214, 354, 389
CviJI RGCY 6 cut(s) 15, 34, 80, 127, 156, 179
CviKI_1 RGCY 6 cut(s) 15, 34, 80, 127, 156, 179
CviQI GTAC 2 cut(s) 232, 413
DpnI GATC 1 cut(s) 257
DpnII GATC 1 cut(s) 255
EaeI YGGCCR 1 cut(s) 177
EagI CGGCCG 1 cut(s) 177
EclXI CGGCCG 1 cut(s) 177
Eco130I CCWWGG 3 cut(s) 122, 353, 364
Eco47I GGWCC 3 cut(s) 145, 197, 361
Eco52I CGGCCG 1 cut(s) 177
Eco88I CYCGRG 1 cut(s) 417
EcoRII CCWGG 1 cut(s) 147
EcoT14I CCWWGG 3 cut(s) 122, 353, 364
ErhI CCWWGG 3 cut(s) 122, 353, 364
Esp3I CGTCTC 1 cut(s) 334
FaeI CATG 5 cut(s) 74, 104, 217, 357, 392
FaiI YATR 6 cut(s) 72, 102, 215, 278, 355, 390
FalI AAGNNNNNCTT 2 cut(s) 115, 147
FatI CATG 5 cut(s) 70, 100, 213, 353, 388
FokI GGATG 3 cut(s) 53, 196, 232
FspBI CTAG 2 cut(s) 365, 404
GsuI CTGGAG 1 cut(s) 170
HaeIII GGCC 2 cut(s) 127, 179
HapII CCGG 3 cut(s) 180, 195, 259
HgaI GACGC 1 cut(s) 288
Hin1I GRCGYC 1 cut(s) 343
Hin1II CATG 5 cut(s) 74, 104, 217, 357, 392
HincII GTYRAC 2 cut(s) 9, 119
HindII GTYRAC 2 cut(s) 9, 119
HinfI GANTC 2 cut(s) 191, 427
HpaII CCGG 3 cut(s) 180, 195, 259
Hpy166II GTNNAC 4 cut(s) 9, 119, 232, 413
Hpy188III TCNNGA 2 cut(s) 259, 419
Hpy8I GTNNAC 4 cut(s) 9, 119, 232, 413
Hpy99I CGWCG 3 cut(s) 321, 348, 351
HpyCH4III ACNGT 1 cut(s) 332
HpyCH4IV ACGT 1 cut(s) 343
HpyCH4V TGCA 4 cut(s) 88, 100, 160, 438
HpySE526I ACGT 1 cut(s) 343
Hsp92I GRCGYC 1 cut(s) 343
Hsp92II CATG 5 cut(s) 74, 104, 217, 357, 392
Kpn2I TCCGGA 1 cut(s) 258
Kzo9I GATC 1 cut(s) 255
LmnI GCTCC 1 cut(s) 390
LpnPI CCDG 6 cut(s) 61, 134, 161, 193, 208, 272
LweI GCATC 2 cut(s) 117, 286
MaeI CTAG 2 cut(s) 365, 404
MaeII ACGT 1 cut(s) 343
MaeIII GTNAC 1 cut(s) 300
MalI GATC 1 cut(s) 257
MboI GATC 1 cut(s) 255
MboII GAAGA 1 cut(s) 123
MnlI CCTC 9 cut(s) 62, 145, 165, 247, 255, 320, 330, 415, 426
MroI TCCGGA 1 cut(s) 258
MslI CAYNNNNRTG 2 cut(s) 218, 294
MspI CCGG 3 cut(s) 180, 195, 259
MspR9I CCNGG 2 cut(s) 149, 181
MvaI CCWGG 1 cut(s) 149
NciI CCSGG 1 cut(s) 181
NcoI CCATGG 1 cut(s) 353
NdeII GATC 1 cut(s) 255
NlaIII CATG 5 cut(s) 74, 104, 217, 357, 392
NmuCI GTSAC 1 cut(s) 300
NspI RCATGY 1 cut(s) 104
PcsI WCGNNNNNNNCGW 1 cut(s) 112
PfeI GAWTC 2 cut(s) 191, 427
PflFI GACNNNGTC 1 cut(s) 345
PfoI TCCNGGA 1 cut(s) 147
Psp6I CCWGG 1 cut(s) 147
PspGI CCWGG 1 cut(s) 147
PspPI GGNCC 3 cut(s) 145, 197, 361
PsyI GACNNNGTC 1 cut(s) 345
RsaI GTAC 2 cut(s) 233, 414
RsaNI GTAC 2 cut(s) 232, 413
RseI CAYNNNNRTG 2 cut(s) 218, 294
Sau3AI GATC 1 cut(s) 255
Sau96I GGNCC 3 cut(s) 145, 197, 361
ScrFI CCNGG 2 cut(s) 149, 181
SetI ASST 6 cut(s) 8, 17, 266, 312, 346, 418
SfaNI GCATC 2 cut(s) 117, 286
SinI GGWCC 3 cut(s) 145, 197, 361
SmiMI CAYNNNNRTG 2 cut(s) 218, 294
SmlI CTYRAG 1 cut(s) 53
SmoI CTYRAG 1 cut(s) 53
SsiI CCGC 1 cut(s) 392
SspMI CTAG 2 cut(s) 365, 404
StyD4I CCNGG 2 cut(s) 147, 179
StyI CCWWGG 3 cut(s) 122, 353, 364
TaaI ACNGT 1 cut(s) 332
TaiI ACGT 1 cut(s) 346
TaqI TCGA 2 cut(s) 189, 248
TaqII GACCGA 3 cut(s) 129, 133, 302
TfiI GAWTC 2 cut(s) 191, 427
TseFI GTSAC 1 cut(s) 300
Tsp45I GTSAC 1 cut(s) 300
TspDTI ATGAA 1 cut(s) 313
Tth111I GACNNNGTC 1 cut(s) 345
VpaK11BI GGWCC 3 cut(s) 145, 197, 361
XceI RCATGY 1 cut(s) 104
XmaJI CCTAGG 1 cut(s) 364
XspI CTAG 2 cut(s) 365, 404
ZraI GACGTC 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.