Rmu_sc0000887.1_g000001

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000887.1
OG0007487
Physical Location & Seq
Forward (+)
1791 .. 2714
924 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 924 bp
atgcaactaactagtgctttgtgggcatgcaagaacgttttactgcaacgtgtagggtatcagacagcaaatcaggaggactgcatgaatgatgcaaagttgctgtccttcgatgatgccagcctcgaaaccagtaccaataagctcattaccaaacttaaggaggatggaggtctttccctggatgttttggagaatgtgctgacaagtatgtctgatttagggcaaaaaactgctgacgcgatcttggatttcaagagtaagatgattgatatgaaggacgatagctataagcttgtggtgatggaatacttgagcagcagcctggagctgctggacttctttactgaattggaaaagtttctagactcggctcgtcggagccattcacatatcgaacatgcgattgaacagtgtagcagcttggggagtaaacatagaccaggaagcgaggaggccaagatgatttccaatatattgagtaacattatggcgtctaatgtcaatgaccactctggtgaaaagttggtcgaaaaagctcattgtttgcttgagaagcagaaagaaatgttttccaaatgtcgcattgaacaagagaagcttgggaaggataagaagagtaatgtttggaggaagctcttgagtcgtatattcatggttgtcaagtggctcgttctcgttgttctaggggtgatattggcctatacgccctataatataacatctgatggagatggtttgtctaatgtggttggagatttgaaaatcaaagcagcatgtttccttgatgtctctgttataacagaaatagtagattactggaatagctcccaccttgaagtatgtcaagatgattttgggctgaagaatgaagcaatgaacataatacaggaaggagctttgaaagaaagaagatttggctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

307

Amino Acids

34.54

Weight (kDa)

5.38

Isoelectric Point (pI)

44.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 36 - 235 7.6e-17 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 800
AasI GACNNNNNNGTC 1 cut(s) 211
AccII CGCG 1 cut(s) 242
AclI AACGTT 1 cut(s) 36
AcuI CTGAAG 1 cut(s) 884
AcyI GRCGYC 1 cut(s) 494
AfaI GTAC 1 cut(s) 136
AflII CTTAAG 1 cut(s) 158
AflIII ACRYGT 1 cut(s) 49
AgsI TTSAA 6 cut(s) 256, 410, 590, 763, 839, 904
AhlI ACTAGT 1 cut(s) 11
AjnI CCWGG 3 cut(s) 180, 324, 442
AjuI GAANNNNNNNTTGG 1 cut(s) 900
AleI CACNNNNGTG 1 cut(s) 516
Alw26I GTCTC 1 cut(s) 796
AoxI GGCC 2 cut(s) 456, 699
ApeKI GCWGC 5 cut(s) 318, 321, 331, 420, 773
ArsI GACNNNNNNTTYG 2 cut(s) 89, 121
Asp700I GAANNNNTTC 1 cut(s) 360
AsuHPI GGTGA 3 cut(s) 313, 530, 703
BbvI GCAGC 5 cut(s) 318, 330, 333, 432, 785
BccI CCATC 4 cut(s) 161, 298, 722, 728
BciT130I CCWGG 3 cut(s) 182, 326, 444
BcoDI GTCTC 1 cut(s) 796
BcuI ACTAGT 1 cut(s) 11
BfaI CTAG 3 cut(s) 12, 365, 686
BfrI CTTAAG 1 cut(s) 158
BisI GCNGC 5 cut(s) 319, 322, 332, 421, 774
BlsI GCNGC 5 cut(s) 320, 323, 333, 422, 775
Bme1390I CCNGG 3 cut(s) 182, 326, 444
BmiI GGNNCC 1 cut(s) 383
BmrFI CCNGG 3 cut(s) 182, 326, 444
BmsI GCATC 2 cut(s) 82, 106
BpmI CTGGAG 1 cut(s) 347
BpuEI CTTGAG 3 cut(s) 334, 572, 661
BsaHI GRCGYC 1 cut(s) 494
BsaJI CCNNGG 1 cut(s) 180
Bse1I ACTGG 2 cut(s) 132, 824
Bse3DI GCAATG 1 cut(s) 882
BseBI CCWGG 3 cut(s) 182, 326, 444
BseDI CCNNGG 1 cut(s) 180
BseGI GGATG 2 cut(s) 172, 190
BseMI GCAATG 1 cut(s) 882
BseNI ACTGG 2 cut(s) 132, 824
BseRI GAGGAG 1 cut(s) 467
BseXI GCAGC 5 cut(s) 318, 330, 333, 432, 785
Bsh1236I CGCG 1 cut(s) 242
BshFI GGCC 2 cut(s) 458, 701
BsmAI GTCTC 1 cut(s) 796
BsnI GGCC 2 cut(s) 458, 701
Bsp143I GATC 1 cut(s) 243
BspANI GGCC 2 cut(s) 458, 701
BspFNI CGCG 1 cut(s) 242
BspLI GGNNCC 1 cut(s) 383
BspTI CTTAAG 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 882
BsrI ACTGG 2 cut(s) 132, 824
BssECI CCNNGG 1 cut(s) 180
BssMI GATC 1 cut(s) 243
BssNI GRCGYC 1 cut(s) 494
Bst2UI CCWGG 3 cut(s) 182, 326, 444
Bst4CI ACNGT 1 cut(s) 414
Bst6I CTCTTC 1 cut(s) 611
BstACI GRCGYC 1 cut(s) 494
BstAFI CTTAAG 1 cut(s) 158
BstC8I GCNNGC 2 cut(s) 28, 121
BstF5I GGATG 2 cut(s) 172, 190
BstFNI CGCG 1 cut(s) 242
BstKTI GATC 1 cut(s) 246
BstMAI GTCTC 1 cut(s) 796
BstMBI GATC 1 cut(s) 243
BstMWI GCNNNNNNNGC 2 cut(s) 23, 556
BstNI CCWGG 3 cut(s) 182, 326, 444
BstNSI RCATGY 3 cut(s) 30, 404, 780
BstSCI CCNGG 3 cut(s) 180, 324, 442
BstUI CGCG 1 cut(s) 242
BstV1I GCAGC 5 cut(s) 318, 330, 333, 432, 785
BsuRI GGCC 2 cut(s) 458, 701
BtsCI GGATG 2 cut(s) 172, 190
BtsIMutI CAGTG 1 cut(s) 419
Cac8I GCNNGC 2 cut(s) 28, 121
CseI GACGC 2 cut(s) 248, 483
Csp6I GTAC 1 cut(s) 135
CviAII CATG 5 cut(s) 27, 85, 401, 655, 777
CviQI GTAC 1 cut(s) 135
DpnI GATC 1 cut(s) 245
DpnII GATC 1 cut(s) 243
DrdI GACNNNNNNGTC 1 cut(s) 211
DseDI GACNNNNNNGTC 1 cut(s) 211
Eam1104I CTCTTC 1 cut(s) 611
EarI CTCTTC 1 cut(s) 611
Eco57I CTGAAG 1 cut(s) 884
EcoRII CCWGG 3 cut(s) 180, 324, 442
FaeI CATG 5 cut(s) 30, 88, 404, 658, 780
FalI AAGNNNNNCTT 2 cut(s) 585, 617
FatI CATG 5 cut(s) 26, 84, 400, 654, 776
Fnu4HI GCNGC 5 cut(s) 319, 322, 332, 421, 774
FokI GGATG 2 cut(s) 179, 197
Fsp4HI GCNGC 5 cut(s) 319, 322, 332, 421, 774
FspBI CTAG 3 cut(s) 12, 365, 686
GluI GCNGC 5 cut(s) 319, 322, 332, 421, 774
GsuI CTGGAG 1 cut(s) 347
HaeIII GGCC 2 cut(s) 458, 701
HgaI GACGC 2 cut(s) 248, 483
Hin1I GRCGYC 1 cut(s) 494
Hin1II CATG 5 cut(s) 30, 88, 404, 658, 780
HindIII AAGCTT 2 cut(s) 293, 599
HinfI GANTC 2 cut(s) 368, 643
HphI GGTGA 3 cut(s) 313, 530, 703
Hpy166II GTNNAC 1 cut(s) 434
Hpy188I TCNGA 4 cut(s) 63, 217, 381, 727
Hpy188III TCNNGA 5 cut(s) 74, 256, 365, 640, 848
Hpy8I GTNNAC 1 cut(s) 434
Hpy99I CGWCG 1 cut(s) 381
HpyAV CCTTC 4 cut(s) 118, 271, 601, 887
HpyCH4III ACNGT 1 cut(s) 414
HpyCH4IV ACGT 2 cut(s) 36, 49
HpyCH4V TGCA 5 cut(s) 4, 30, 46, 84, 95
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 556
HpySE526I ACGT 2 cut(s) 36, 49
Hsp92I GRCGYC 1 cut(s) 494
Hsp92II CATG 5 cut(s) 30, 88, 404, 658, 780
Kzo9I GATC 1 cut(s) 243
LmnI GCTCC 4 cut(s) 328, 381, 833, 896
Lsp1109I GCAGC 5 cut(s) 318, 330, 333, 432, 785
LweI GCATC 2 cut(s) 82, 106
MaeI CTAG 3 cut(s) 12, 365, 686
MaeII ACGT 2 cut(s) 36, 49
MaeIII GTNAC 1 cut(s) 482
MalI GATC 1 cut(s) 245
MboI GATC 1 cut(s) 243
MboII GAAGA 3 cut(s) 628, 877, 924
MluCI AATT 1 cut(s) 350
MlyI GAGTC 2 cut(s) 362, 652
MmeI TCCRAC 2 cut(s) 359, 733
MnlI CCTC 7 cut(s) 70, 134, 157, 164, 445, 448, 624
MroXI GAANNNNTTC 1 cut(s) 360
MseI TTAA 1 cut(s) 159
MslI CAYNNNNRTG 1 cut(s) 516
MspCI CTTAAG 1 cut(s) 158
MspR9I CCNGG 3 cut(s) 182, 326, 444
MvaI CCWGG 3 cut(s) 182, 326, 444
MvnI CGCG 1 cut(s) 242
MwoI GCNNNNNNNGC 2 cut(s) 23, 556
NdeII GATC 1 cut(s) 243
NlaIII CATG 5 cut(s) 30, 88, 404, 658, 780
NlaIV GGNNCC 1 cut(s) 383
NmeAIII GCCGAG 1 cut(s) 350
NspI RCATGY 3 cut(s) 30, 404, 780
OliI CACNNNNGTG 1 cut(s) 516
PaeI GCATGC 1 cut(s) 30
PdmI GAANNNNTTC 1 cut(s) 360
PkrI GCNGC 5 cut(s) 320, 323, 333, 422, 775
PleI GAGTC 2 cut(s) 362, 651
PpsI GAGTC 2 cut(s) 362, 651
PsiI TTATAA 1 cut(s) 800
Psp1406I AACGTT 1 cut(s) 36
Psp6I CCWGG 3 cut(s) 180, 324, 442
PspGI CCWGG 3 cut(s) 180, 324, 442
PspN4I GGNNCC 1 cut(s) 383
RsaI GTAC 1 cut(s) 136
RsaNI GTAC 1 cut(s) 135
RseI CAYNNNNRTG 1 cut(s) 516
SaqAI TTAA 1 cut(s) 159
SatI GCNGC 5 cut(s) 319, 322, 332, 421, 774
Sau3AI GATC 1 cut(s) 243
SchI GAGTC 2 cut(s) 362, 652
ScrFI CCNGG 3 cut(s) 182, 326, 444
SfaNI GCATC 2 cut(s) 82, 106
SmiMI CAYNNNNRTG 1 cut(s) 516
SmlI CTYRAG 4 cut(s) 158, 313, 551, 640
SmoI CTYRAG 4 cut(s) 158, 313, 551, 640
SpeI ACTAGT 1 cut(s) 11
SphI GCATGC 1 cut(s) 30
Sse9I AATT 1 cut(s) 350
SspMI CTAG 3 cut(s) 12, 365, 686
StyD4I CCNGG 3 cut(s) 180, 324, 442
TaaI ACNGT 1 cut(s) 414
TaiI ACGT 2 cut(s) 39, 52
TaqI TCGA 4 cut(s) 111, 126, 396, 531
TasI AATT 1 cut(s) 350
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TscAI CASTG 1 cut(s) 419
TseI GCWGC 5 cut(s) 318, 321, 331, 420, 773
TspDTI ATGAA 5 cut(s) 101, 290, 643, 885, 893
TspRI CASTG 1 cut(s) 419
Vha464I CTTAAG 1 cut(s) 158
XbaI TCTAGA 1 cut(s) 364
XceI RCATGY 3 cut(s) 30, 404, 780
XmnI GAANNNNTTC 1 cut(s) 360
XspI CTAG 3 cut(s) 12, 365, 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.