Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0001275.1

Orthogroup ID

OG0002975

Physical Location & Seq

Genomic Coordinates Forward (+)

116991 .. 121902

Gene Structure 4912 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2283 bp

atgcccatgtcaagtgaggctaacggtgagcatgacaagaggccaaaatcagcaccagcgcacgttatcacgctccgcctaaaacctcccatctattttcttcttcttcttcttcttcttccaccacttaccacactctccgaccgtccattcccggccgtccgattcgccggaatggaggctctgttcgactcaatcaacgtccgagagctcctctcggcgcaggacctcagcgaccccaccacacctctctccgcaccggatctccgcctcctcatccaacgcctcgactcccactccctcaaaataaagtccaaaatccaatcctacctcctctctcaccaacacgacttcgccgacctcttctccctctgcaacgacgccgtttcccgctccgttaaaatctccgacgacgtcgttcagcttctcgcctcactctccgaccgccccgtcgaggcagagatcggccagattatgaagcagatgagcgccaccactaaagaggcgagggagaagcgggagctgctggagttggtgagggcaattctggaaataagcgaaaaattgaaggcggctagagaggcgttgaggaaggggcggctgagatttgcggctgagcaattgagggagctgaagaaggctcttagggtttccgatgatgatcgggttgatgagagtgagcctgtggtttatggcttgttgaggaaggagtggtcggactgctttgatgagattcaagaggtgctgatgaggttcatggacaatgcggtgcgtttcgatcgcgaatcgaatagaattcaagtcaagtatgtactcagcattggtggcaacgatgggattgagctcaagacagtgttacaagctttggatgtggttgacattttacactatggacttgctaaagtagctgacttgataatcaagcattgcatctctccacctctaaattctggagcacctgtgtcctttgtaacagaaataaatccaaactcacaagcaatgactgaggcgatactgaagattgtaccatcaaatgatcctaaggttgtcccccaagatgcttcaaaacttgcagactttctaaagatcatcaattgtacttctgaatttgagactgctttaagggagatgaagtttatttcagtatctgaaaacaaagataaccagttgagcagttttgctgaaaacgtcgaggttcacttcgcatccaggaagaagacagaaatcttgggaagagctaggaatttgcttctacaatgtgattttgctatttctcaagagaatacaaggaggaaaggtaagcacgatggggctgctagcaatacaccagaagatgttgacttgctatttctttcggagcggtgtgtggtgtccaaagcagcaattcaattaatgaagttagtgcatgagacactcaaggatgtctgcttgtcatcccctagagttgcattggaattttatcgtgctgctagggatgtactacttctatatgaagttgtcatacctgtcaagctagaacggcagcttgatggcatcaaccaggttgctgttctgatgcacaacgattgcctttttctatctcaggagattcttgggcttgcatttgagtatcgctcagactttccaacttcaatgaaagatcatgctgtatttgttgatatggctccaagatttcatctcatggcagagggaatactgcagagacaaatccaacttgtgattcatagcttgagagaggctgtagatggcgctgatggattccagaatacccatcaaatgcaacaattccaatctgcaaaatttagtatagatcaggttgttttcatattggagaaagtacacattatctgggagccactcttattaccttcaacttacaagagaagcatgtgtctgattctagagtcagttttttctagaatcacaaaagacatactccttttagatgatatggcagcagaagaaacgttagaggttcaaagactgattcacttgatgctggaaaacgtaacttctttgttggggtccctggctgctcttcaagttgagaagtcacaagcgggcatgacacctcttgatgatcttataccatcattacgaaaaattcggaaattggcagaattattagatatgcccttgaaagcaatcactacagcttgggaaactggagaactgcgaaatggtggctttacattgtcagaggtagaatatttcatcaaagcaatttttcaagactcgcctttgcggaaagactgcttatggaggatacatggctactcttaa

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000075 GO:0000278 GO:0000775 GO:0000776 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0006810 GO:0006888 GO:0007049 GO:0007059 GO:0007088 GO:0007093 GO:0007094 GO:0007346 GO:0008150 GO:0009987 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0016192 GO:0022402 GO:0030071 GO:0031577 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0046907 GO:0048193 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0065007 GO:0071173 GO:0071174 GO:0098687 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1903047 GO:1905818 GO:1905819 GO:1990423 GO:2000816 GO:2001251

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Zw10

Protein Analysis

760

Amino Acids

85.83

Weight (kDa)

5.8

Isoelectric Point (pI)

41.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Zw10_N	PF06248	96 - 189	5e-12	Centromere/kinetochore Zw10 N-terminal
Zw10_middle	PF20665	234 - 325	2e-10	Centromere/kinetochore protein zw10, middle domain
Zw10_middle	PF20665	348 - 417	4.7e-17	Centromere/kinetochore protein zw10, middle domain
ZW10_C	PF20666	450 - 579	7.1e-40	Centromere/kinetochore protein zw10, C-terminal
ZW10_C2	PF22766	602 - 756	3.7e-39	ZW10 C-terminal helical domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002975)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32900
fragaria_vesca	FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900
malus_domestica	MD09G1116200.v1.1 MD17G1107900.v1.1
prunus_persica	Prupe.3G208500_v2.0.a1
pyrus_communis	pycom09g04060 pycom17g10050
rosa_chinensis	RchiOBHm_Chr0c20g0500271 RchiOBHm_Chr1g0340131 RchiOBHm_Chr1g0340141 RchiOBHm_Chr2g0157781
rosa_laevigata	RLG00000021018 RLG00000028993
rosa_multiflora	Rmu_co8468091.1_g000001 Rmu_sc0001275.1_g000033 Rmu_sc0010800.1_g000004 Rmu_sc0018661.1_g000001
rosa_roxburghii	Rroxscaffold_2G00091960 Rroxscaffold_4G00311000
rosa_rugosa	Rorug02G0463300 Rorug02G0463300
rosa_samantha	Rh1AG180900 Rh1CG167700 Rh1DG181000 Rh1DG181100 Rh2AG528800 Rh2BG542700 Rh2CG513100 Rh2DG551700
rosa_wichuraiana	Rw1G015020 Rw2G043720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	449
AatII	GACGTC	1 cut(s)	417
AccBSI	CCGCTC	2 cut(s)	393, 1360
AccII	CGCG	1 cut(s)	783
AclI	AACGTT	1 cut(s)	1967
AclWI	GGATC	2 cut(s)	270, 1031
AcoI	YGGCCR	2 cut(s)	156, 466
AcsI	RAATTY	7 cut(s)	795, 946, 1106, 1243, 1454, 1799, 2103
AcuI	CTGAAG	2 cut(s)	653, 1037
AcyI	GRCGYC	2 cut(s)	381, 414
AfaI	GTAC	5 cut(s)	813, 1026, 1099, 1479, 1839
AfiI	CCNNNNNNNGG	1 cut(s)	454
AjnI	CCWGG	3 cut(s)	1208, 1539, 2028
AjuI	GAANNNNNNNTTGG	2 cut(s)	1704, 1736
AloI	GAACNNNNNNTCC	2 cut(s)	170, 202
Alw21I	GWGCWC	3 cut(s)	213, 846, 958
Alw26I	GTCTC	3 cut(s)	1106, 1403, 1696
AlwI	GGATC	2 cut(s)	270, 1031
AlwNI	CAGNNNCTG	1 cut(s)	1148
AoxI	GGCC	3 cut(s)	41, 156, 466
ApeKI	GCWGC	7 cut(s)	523, 1313, 1379, 1466, 1522, 1955, 2033
ApoI	RAATTY	7 cut(s)	795, 946, 1106, 1243, 1454, 1799, 2103
AseI	ATTAAT	1 cut(s)	1391
AspLEI	GCGC	4 cut(s)	61, 223, 491, 1751
AspS9I	GGNCC	2 cut(s)	226, 2025
AsuC2I	CCSGG	1 cut(s)	155
AsuHPI	GGTGA	3 cut(s)	38, 332, 547
AsuNHI	GCTAGC	1 cut(s)	1316
AvaII	GGWCC	2 cut(s)	226, 2025
AxyI	CCTNAGG	1 cut(s)	1041
BanII	GRGCYC	2 cut(s)	213, 846
BarI	GAAGNNNNNNTAC	2 cut(s)	1485, 1517
BbsI	GAAGAC	1 cut(s)	1223
Bbv12I	GWGCWC	3 cut(s)	213, 846, 958
BbvCI	CCTCAGC	1 cut(s)	230
BbvI	GCAGC	7 cut(s)	510, 1300, 1391, 1453, 1534, 1967, 2020
BccI	CCATC	9 cut(s)	98, 827, 1036, 1301, 1523, 1739, 1748, 1779, 2098
BceAI	ACGGC	3 cut(s)	143, 368, 1535
BcgI	CGANNNNNNTGC	2 cut(s)	1295, 1329
BciT130I	CCWGG	3 cut(s)	1210, 1541, 2030
BciVI	GTATCC	1 cut(s)	2259
BcnI	CCSGG	1 cut(s)	155
BcoDI	GTCTC	3 cut(s)	1106, 1403, 1696
BfaI	CTAG	8 cut(s)	576, 1239, 1317, 1440, 1470, 1514, 1901, 1917
BfmI	CTRYAG	3 cut(s)	1697, 1740, 2151
BfoI	RGCGCY	2 cut(s)	492, 1752
BfuI	GTATCC	1 cut(s)	2259
BlpI	GCTNAGC	1 cut(s)	615
Bme1390I	CCNGG	4 cut(s)	155, 1210, 1541, 2030
Bme18I	GGWCC	2 cut(s)	226, 2025
BmgT120I	GGNCC	2 cut(s)	226, 2025
BmiI	GGNNCC	4 cut(s)	1665, 1854, 2026, 2027
BmrFI	CCNGG	4 cut(s)	155, 1210, 1541, 2030
BmsI	GCATC	6 cut(s)	939, 1048, 1214, 1542, 1545, 1986
BmtI	GCTAGC	1 cut(s)	1320
BpiI	GAAGAC	1 cut(s)	1223
BplI	GAGNNNNNCTC	6 cut(s)	198, 230, 200, 232, 1598, 1630
BpmI	CTGGAG	3 cut(s)	548, 972, 2187
Bpu10I	CCTNAGC	1 cut(s)	230
Bpu1102I	GCTNAGC	1 cut(s)	615
BpuEI	CTTGAG	4 cut(s)	830, 1260, 1400, 1750
BpuMI	CCSGG	1 cut(s)	155
BsaBI	GATNNNNATC	1 cut(s)	660
BsaHI	GRCGYC	2 cut(s)	381, 414
BsaJI	CCNNGG	1 cut(s)	2028
BsaWI	WCCGGW	1 cut(s)	259
Bsc4I	CCNNNNNNNGG	1 cut(s)	454
Bse1I	ACTGG	2 cut(s)	1165, 2170
Bse21I	CCTNAGG	1 cut(s)	1041
Bse3DI	GCAATG	2 cut(s)	925, 1005
Bse8I	GATNNNNATC	1 cut(s)	660
BseBI	CCWGG	3 cut(s)	1210, 1541, 2030
BseDI	CCNNGG	1 cut(s)	2028
BseGI	GGATG	6 cut(s)	276, 874, 1205, 1426, 1433, 1480
BseJI	GATNNNNATC	1 cut(s)	660
BseLI	CCNNNNNNNGG	1 cut(s)	454
BseMI	GCAATG	2 cut(s)	925, 1005
BseMII	CTCAG	7 cut(s)	244, 593, 606, 829, 996, 1595, 1629
BseNI	ACTGG	2 cut(s)	1165, 2170
BseRI	GAGGAG	3 cut(s)	203, 263, 323
BseX3I	CGGCCG	1 cut(s)	156
BseXI	GCAGC	7 cut(s)	510, 1300, 1391, 1453, 1534, 1967, 2020
Bsh1236I	CGCG	1 cut(s)	783
Bsh1285I	CGRYCG	4 cut(s)	145, 159, 445, 781
BshFI	GGCC	3 cut(s)	43, 158, 468
BsiEI	CGRYCG	4 cut(s)	145, 159, 445, 781
BsiHKAI	GWGCWC	3 cut(s)	213, 846, 958
BsiSI	CCGG	3 cut(s)	155, 171, 260
BslFI	GGGAC	2 cut(s)	1034, 2011
BslI	CCNNNNNNNGG	1 cut(s)	454
BsmAI	GTCTC	3 cut(s)	1106, 1403, 1696
BsmFI	GGGAC	2 cut(s)	1034, 2011
BsnI	GGCC	3 cut(s)	43, 158, 468
Bsp1286I	GDGCHC	3 cut(s)	213, 846, 958
Bsp143I	GATC	9 cut(s)	262, 462, 661, 778, 1036, 1086, 1639, 1810, 2080
Bsp1720I	GCTNAGC	1 cut(s)	615
Bsp68I	TCGCGA	1 cut(s)	783
BspANI	GGCC	3 cut(s)	43, 158, 468
BspCNI	CTCAG	7 cut(s)	243, 594, 607, 828, 997, 1594, 1628
BspFNI	CGCG	1 cut(s)	783
BspLI	GGNNCC	4 cut(s)	1665, 1854, 2026, 2027
BspMAI	CTGCAG	1 cut(s)	1701
BspOI	GCTAGC	1 cut(s)	1320
BspPI	GGATC	2 cut(s)	270, 1031
BspQI	GCTCTTC	2 cut(s)	1228, 2043
BsrBI	CCGCTC	2 cut(s)	393, 1360
BsrDI	GCAATG	2 cut(s)	925, 1005
BsrI	ACTGG	2 cut(s)	1165, 2170
BssECI	CCNNGG	1 cut(s)	2028
BssMI	GATC	9 cut(s)	262, 462, 661, 778, 1036, 1086, 1639, 1810, 2080
BssNI	GRCGYC	2 cut(s)	381, 414
Bst2UI	CCWGG	3 cut(s)	1210, 1541, 2030
Bst4CI	ACNGT	3 cut(s)	26, 146, 853
Bst6I	CTCTTC	3 cut(s)	368, 1228, 2043
BstACI	GRCGYC	2 cut(s)	381, 414
BstC8I	GCNNGC	3 cut(s)	1318, 1599, 2062
BstDEI	CTNAG	9 cut(s)	230, 602, 615, 644, 815, 1005, 1041, 1581, 1615
BstF5I	GGATG	6 cut(s)	276, 874, 1205, 1426, 1433, 1480
BstFNI	CGCG	1 cut(s)	783
BstH2I	RGCGCY	2 cut(s)	492, 1752
BstHHI	GCGC	4 cut(s)	61, 223, 491, 1751
BstKTI	GATC	9 cut(s)	265, 465, 664, 781, 1039, 1089, 1642, 1813, 2083
BstMAI	GTCTC	3 cut(s)	1106, 1403, 1696
BstMBI	GATC	9 cut(s)	262, 462, 661, 778, 1036, 1086, 1639, 1810, 2080
BstMCI	CGRYCG	4 cut(s)	145, 159, 445, 781
BstMWI	GCNNNNNNNGC	5 cut(s)	523, 581, 825, 905, 1519
BstNI	CCWGG	3 cut(s)	1210, 1541, 2030
BstNSI	RCATGY	1 cut(s)	1891
BstSCI	CCNGG	4 cut(s)	153, 1208, 1539, 2028
BstSFI	CTRYAG	3 cut(s)	1697, 1740, 2151
BstUI	CGCG	1 cut(s)	783
BstV1I	GCAGC	7 cut(s)	510, 1300, 1391, 1453, 1534, 1967, 2020
BstV2I	GAAGAC	1 cut(s)	1223
BstX2I	RGATCY	1 cut(s)	262
BstYI	RGATCY	1 cut(s)	262
BstZI	CGGCCG	1 cut(s)	156
Bsu36I	CCTNAGG	1 cut(s)	1041
BsuI	GTATCC	1 cut(s)	2259
BsuRI	GGCC	3 cut(s)	43, 158, 468
BtsCI	GGATG	6 cut(s)	276, 874, 1205, 1426, 1433, 1480
BtsIMutI	CAGTG	1 cut(s)	858
BtuMI	TCGCGA	1 cut(s)	783
Cac8I	GCNNGC	3 cut(s)	1318, 1599, 2062
CaiI	CAGNNNCTG	1 cut(s)	1148
CfoI	GCGC	4 cut(s)	61, 223, 491, 1751
Cfr13I	GGNCC	2 cut(s)	226, 2025
CseI	GACGC	1 cut(s)	389
CsiI	ACCWGGT	1 cut(s)	1539
Csp6I	GTAC	5 cut(s)	812, 1025, 1098, 1478, 1838
CviAII	CATG	9 cut(s)	7, 32, 757, 1406, 1643, 1681, 1888, 2065, 2270
CviQI	GTAC	5 cut(s)	812, 1025, 1098, 1478, 1838
DdeI	CTNAG	9 cut(s)	230, 602, 615, 644, 815, 1005, 1041, 1581, 1615
DpnI	GATC	9 cut(s)	264, 464, 663, 780, 1038, 1088, 1641, 1812, 2082
DpnII	GATC	9 cut(s)	262, 462, 661, 778, 1036, 1086, 1639, 1810, 2080
DrdI	GACNNNNNNGTC	1 cut(s)	449
DseDI	GACNNNNNNGTC	1 cut(s)	449
EaeI	YGGCCR	2 cut(s)	156, 466
EagI	CGGCCG	1 cut(s)	156
Eam1104I	CTCTTC	3 cut(s)	368, 1228, 2043
EarI	CTCTTC	3 cut(s)	368, 1228, 2043
EciI	GGCGGA	2 cut(s)	65, 257
Ecl136II	GAGCTC	2 cut(s)	211, 844
EclXI	CGGCCG	1 cut(s)	156
Eco24I	GRGCYC	2 cut(s)	213, 846
Eco47I	GGWCC	2 cut(s)	226, 2025
Eco52I	CGGCCG	1 cut(s)	156
Eco53kI	GAGCTC	2 cut(s)	211, 844
Eco57I	CTGAAG	2 cut(s)	653, 1037
Eco81I	CCTNAGG	1 cut(s)	1041
EcoICRI	GAGCTC	2 cut(s)	211, 844
EcoO109I	RGGNCCY	2 cut(s)	226, 2025
EcoRI	GAATTC	1 cut(s)	795
EcoRII	CCWGG	3 cut(s)	1208, 1539, 2028
EcoT38I	GRGCYC	2 cut(s)	213, 846
FaeI	CATG	9 cut(s)	10, 35, 760, 1409, 1646, 1684, 1891, 2068, 2273
FaqI	GGGAC	2 cut(s)	1034, 2011
FatI	CATG	9 cut(s)	6, 31, 756, 1405, 1642, 1680, 1887, 2064, 2269
FauI	CCCGC	3 cut(s)	398, 510, 2053
FokI	GGATG	6 cut(s)	263, 881, 1192, 1420, 1433, 1487
FriOI	GRGCYC	2 cut(s)	213, 846
FspBI	CTAG	8 cut(s)	576, 1239, 1317, 1440, 1470, 1514, 1901, 1917
GlaI	GCGC	4 cut(s)	60, 222, 490, 1750
GsuI	CTGGAG	3 cut(s)	548, 972, 2187
HaeII	RGCGCY	2 cut(s)	492, 1752
HaeIII	GGCC	3 cut(s)	43, 158, 468
HapII	CCGG	3 cut(s)	155, 171, 260
HgaI	GACGC	1 cut(s)	389
HhaI	GCGC	4 cut(s)	61, 223, 491, 1751
Hin1I	GRCGYC	2 cut(s)	381, 414
Hin1II	CATG	9 cut(s)	10, 35, 760, 1409, 1646, 1684, 1891, 2068, 2273
Hin6I	GCGC	4 cut(s)	59, 221, 489, 1749
HinP1I	GCGC	4 cut(s)	59, 221, 489, 1749
HincII	GTYRAC	2 cut(s)	877, 1339
HindII	GTYRAC	2 cut(s)	877, 1339
HindIII	AAGCTT	1 cut(s)	861
HpaII	CCGG	3 cut(s)	155, 171, 260
HphI	GGTGA	3 cut(s)	38, 332, 547
Hpy166II	GTNNAC	4 cut(s)	877, 1198, 1339, 1840
Hpy8I	GTNNAC	4 cut(s)	877, 1198, 1339, 1840
Hpy99I	CGWCG	6 cut(s)	383, 413, 416, 419, 455, 1193
HpyAV	CCTTC	5 cut(s)	562, 586, 631, 700, 1878
HpyCH4III	ACNGT	3 cut(s)	26, 146, 853
HpyCH4IV	ACGT	6 cut(s)	63, 201, 414, 1188, 1967, 2007
HpyF10VI	GCNNNNNNNGC	5 cut(s)	523, 581, 825, 905, 1519
HpyF3I	CTNAG	9 cut(s)	230, 602, 615, 644, 815, 1005, 1041, 1581, 1615
HpySE526I	ACGT	6 cut(s)	63, 201, 414, 1188, 1967, 2007
Hsp92I	GRCGYC	2 cut(s)	381, 414
Hsp92II	CATG	9 cut(s)	10, 35, 760, 1409, 1646, 1684, 1891, 2068, 2273
HspAI	GCGC	4 cut(s)	59, 221, 489, 1749
KflI	GGGWCCC	1 cut(s)	2025
Kzo9I	GATC	9 cut(s)	262, 462, 661, 778, 1036, 1086, 1639, 1810, 2080
LguI	GCTCTTC	2 cut(s)	1228, 2043
LmnI	GCTCC	9 cut(s)	78, 216, 398, 520, 628, 953, 1357, 1669, 1852
Lsp1109I	GCAGC	7 cut(s)	510, 1300, 1391, 1453, 1534, 1967, 2020
LweI	GCATC	6 cut(s)	939, 1048, 1214, 1542, 1545, 1986
MabI	ACCWGGT	1 cut(s)	1539
MaeI	CTAG	8 cut(s)	576, 1239, 1317, 1440, 1470, 1514, 1901, 1917
MaeII	ACGT	6 cut(s)	63, 201, 414, 1188, 1967, 2007
MaeIII	GTNAC	4 cut(s)	855, 970, 2008, 2052
MalI	GATC	9 cut(s)	264, 464, 663, 780, 1038, 1088, 1641, 1812, 2082
MbiI	CCGCTC	2 cut(s)	393, 1360
MboI	GATC	9 cut(s)	262, 462, 661, 778, 1036, 1086, 1639, 1810, 2080
MfeI	CAATTG	2 cut(s)	620, 1093
MflI	RGATCY	1 cut(s)	262
MhlI	GDGCHC	3 cut(s)	213, 846, 958
MlyI	GAGTC	4 cut(s)	185, 284, 1913, 2228
MmeI	TCCRAC	7 cut(s)	165, 304, 432, 465, 696, 1649, 1735
MseI	TTAA	4 cut(s)	399, 1121, 1391, 2281
MspI	CCGG	3 cut(s)	155, 171, 260
MspR9I	CCNGG	4 cut(s)	155, 1210, 1541, 2030
MunI	CAATTG	2 cut(s)	620, 1093
MvaI	CCWGG	3 cut(s)	1210, 1541, 2030
MvnI	CGCG	1 cut(s)	783
MwoI	GCNNNNNNNGC	5 cut(s)	523, 581, 825, 905, 1519
NciI	CCSGG	1 cut(s)	155
NdeII	GATC	9 cut(s)	262, 462, 661, 778, 1036, 1086, 1639, 1810, 2080
NheI	GCTAGC	1 cut(s)	1316
NlaIII	CATG	9 cut(s)	10, 35, 760, 1409, 1646, 1684, 1891, 2068, 2273
NlaIV	GGNNCC	4 cut(s)	1665, 1854, 2026, 2027
NmeAIII	GCCGAG	1 cut(s)	197
NmuCI	GTSAC	1 cut(s)	2052
NruI	TCGCGA	1 cut(s)	783
NspI	RCATGY	1 cut(s)	1891
PciSI	GCTCTTC	2 cut(s)	1228, 2043
PcsI	WCGNNNNNNNCGW	1 cut(s)	354
PfeI	GAWTC	9 cut(s)	165, 733, 785, 1588, 1720, 1758, 1897, 1920, 1987
PflFI	GACNNNGTC	1 cut(s)	413
PfoI	TCCNGGA	1 cut(s)	1208
Ple19I	CGATCG	1 cut(s)	781
PleI	GAGTC	4 cut(s)	185, 284, 1912, 2228
PpsI	GAGTC	4 cut(s)	185, 284, 1912, 2228
PpuMI	RGGWCCY	2 cut(s)	226, 2025
PshBI	ATTAAT	1 cut(s)	1391
Psp124BI	GAGCTC	2 cut(s)	213, 846
Psp1406I	AACGTT	1 cut(s)	1967
Psp5II	RGGWCCY	2 cut(s)	226, 2025
Psp6I	CCWGG	3 cut(s)	1208, 1539, 2028
PspGI	CCWGG	3 cut(s)	1208, 1539, 2028
PspN4I	GGNNCC	4 cut(s)	1665, 1854, 2026, 2027
PspPI	GGNCC	2 cut(s)	226, 2025
PspPPI	RGGWCCY	2 cut(s)	226, 2025
PstI	CTGCAG	1 cut(s)	1701
PstNI	CAGNNNCTG	1 cut(s)	1148
PsuI	RGATCY	1 cut(s)	262
PsyI	GACNNNGTC	1 cut(s)	413
PvuI	CGATCG	1 cut(s)	781
RruI	TCGCGA	1 cut(s)	783
RsaI	GTAC	5 cut(s)	813, 1026, 1099, 1479, 1839
RsaNI	GTAC	5 cut(s)	812, 1025, 1098, 1478, 1838
SacI	GAGCTC	2 cut(s)	213, 846
SapI	GCTCTTC	2 cut(s)	1228, 2043
SaqAI	TTAA	4 cut(s)	399, 1121, 1391, 2281
Sau3AI	GATC	9 cut(s)	262, 462, 661, 778, 1036, 1086, 1639, 1810, 2080
Sau96I	GGNCC	2 cut(s)	226, 2025
SchI	GAGTC	4 cut(s)	185, 284, 1913, 2228
ScrFI	CCNGG	4 cut(s)	155, 1210, 1541, 2030
SduI	GDGCHC	3 cut(s)	213, 846, 958
SexAI	ACCWGGT	1 cut(s)	1539
SfaNI	GCATC	6 cut(s)	939, 1048, 1214, 1542, 1545, 1986
SfcI	CTRYAG	3 cut(s)	1697, 1740, 2151
SinI	GGWCC	2 cut(s)	226, 2025
SmlI	CTYRAG	4 cut(s)	845, 1275, 1415, 1729
SmoI	CTYRAG	4 cut(s)	845, 1275, 1415, 1729
SspI	AATATT	1 cut(s)	2210
SspMI	CTAG	8 cut(s)	576, 1239, 1317, 1440, 1470, 1514, 1901, 1917
SstI	GAGCTC	2 cut(s)	213, 846
StyD4I	CCNGG	4 cut(s)	153, 1208, 1539, 2028
TaaI	ACNGT	3 cut(s)	26, 146, 853
TaiI	ACGT	6 cut(s)	66, 204, 417, 1191, 1970, 2010
TaqI	TCGA	6 cut(s)	189, 288, 453, 777, 788, 1191
TatI	WGTACW	4 cut(s)	811, 1097, 1477, 1837
TauI	GCSGC	3 cut(s)	575, 601, 614
TfiI	GAWTC	9 cut(s)	165, 733, 785, 1588, 1720, 1758, 1897, 1920, 1987
Tru1I	TTAA	4 cut(s)	399, 1121, 1391, 2281
Tru9I	TTAA	4 cut(s)	399, 1121, 1391, 2281
TscAI	CASTG	1 cut(s)	858
TseFI	GTSAC	1 cut(s)	2052
TseI	GCWGC	7 cut(s)	523, 1313, 1379, 1466, 1522, 1955, 2033
Tsp45I	GTSAC	1 cut(s)	2052
TspGWI	ACGGA	1 cut(s)	385
TspRI	CASTG	1 cut(s)	858
Tth111I	GACNNNGTC	1 cut(s)	413
VpaK11BI	GGWCC	2 cut(s)	226, 2025
VspI	ATTAAT	1 cut(s)	1391
XapI	RAATTY	7 cut(s)	795, 946, 1106, 1243, 1454, 1799, 2103
XbaI	TCTAGA	2 cut(s)	1900, 1916
XceI	RCATGY	1 cut(s)	1891
XspI	CTAG	8 cut(s)	576, 1239, 1317, 1440, 1470, 1514, 1901, 1917
ZraI	GACGTC	1 cut(s)	415

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.