Rmu_sc0001576.1_g000027

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001576.1
OG0012764
Physical Location & Seq
Reverse (-)
112705 .. 115716
3012 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1173 bp
atggcagctccaccatgtccacgcgcacgacaccaccacccctgccgtctgcgtcaaaccgtacccatggctcaccacgaatcccagcgactcaatctgagacagaaccgccacgggcagcggagctccggaggtcatgatctgcttttggctcggcttggacaattcgaattcggctcggcttggacaattcgaactgcctttcaatacccagccgatcggtttcggcgcgggtcggacgggatcgtggatcggaaacatgggaagaggccacttccttcgaatgaatcggagaagaaagaggaggaggtggagtatcatcgaaatatcttcccggtttactcaagtagatctcaacaagataattctgccatggtatcagctctatctcaggtgattgcaaacgctcatgaacaaaacccgcttcaacttgatcatcaagttcatggtatcccactaataatttcagcagataatcatgttgagcaatctaaacctgcagtagtttttcaagctcaagggaatatgagacaacactataggggagtgaggcggaggccatgggggaagtgggcagccgagattcgtgatccgcagaaggcagctcgagtgtggctcgggactttcgaatcagcagaggctgcagcacttgcttacgatgaagcagctctcaggttcaaaggaagcaaggctaagcttaacttccccgaaagagttcaagggatcagccctagtacttctagtggtcgctaccttgcaataagtagtatcgatcatgatcagcgagtagacaattcagctcctccagctgcgccgatttctcaactaacaacctatagtattccaaatgatattaattttgattatgatgcacaacatcttgggagtagtactacaagtaacagaaatgttgattttggtgtatcatcatctcaaaactacggacaagaaagaattatgccggagattaatgtaccaacgacttcttcttctacatcctcgggctcttccatgccatctcatcatcaagaagtacgactacagcagcaagaagatcagcagcaactcttaaggcttccgatgatgccctttggcggttcatcttcgagctcatcagatcctcctgctaagtacaggagagactcctctgggaggggtgactatagaagatga

Protein Analysis

390

Amino Acids

43.7

Weight (kDa)

9.6

Isoelectric Point (pI)

61.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 179 - 228 1.4e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012764)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07310 AT5G61890
fragaria_vesca FvH4_5g09520
malus_domestica MD06G1130100.v1.1 MD06G1130300.v1.1 MD14G1147100.v1.1
prunus_persica Prupe.5G141200_v2.0.a1
pyrus_communis pycom14g12170
rosa_chinensis RchiOBHm_Chr7g0188681
rosa_laevigata RLG00000004671
rosa_multiflora Rmu_sc0001576.1_g000027
rosa_roxburghii Rroxscaffold_3G00266140
rosa_rugosa Rorug06G0492200
rosa_samantha Rh7AG097100 Rh7BG098900 Rh7CG100700 Rh7DG099300
rosa_wichuraiana Rw7G008400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 505
AccI GTMKAC 1 cut(s) 789
AccII CGCG 2 cut(s) 24, 231
AccIII TCCGGA 1 cut(s) 128
AciI CCGC 7 cut(s) 109, 121, 231, 422, 553, 593, 1095
AclWI GGATC 5 cut(s) 251, 258, 584, 731, 1112
AcsI RAATTY 1 cut(s) 170
AfaI GTAC 6 cut(s) 63, 736, 892, 975, 1035, 1133
AfiI CCNNNNNNNGG 2 cut(s) 1094, 1152
AflII CTTAAG 1 cut(s) 1069
AgsI TTSAA 5 cut(s) 206, 428, 512, 679, 719
AjuI GAANNNNNNNTTGG 2 cut(s) 970, 1002
Alw21I GWGCWC 2 cut(s) 128, 1112
Alw26I GTCTC 3 cut(s) 94, 523, 1134
AlwI GGATC 5 cut(s) 251, 258, 584, 731, 1112
AlwNI CAGNNNCTG 1 cut(s) 641
Ama87I CYCGRG 3 cut(s) 606, 617, 1000
Aor13HI TCCGGA 1 cut(s) 128
AoxI GGCC 2 cut(s) 269, 557
ApoI RAATTY 1 cut(s) 170
AseI ATTAAT 2 cut(s) 855, 969
Asp700I GAANNNNTTC 1 cut(s) 714
AspLEI GCGC 3 cut(s) 26, 231, 814
AsuC2I CCSGG 1 cut(s) 335
AsuHPI GGTGA 3 cut(s) 65, 406, 1169
AsuII TTCGAA 4 cut(s) 168, 193, 281, 627
AvaI CYCGRG 3 cut(s) 606, 617, 1000
BanII GRGCYC 3 cut(s) 128, 1007, 1112
BarI GAAGNNNNNNTAC 2 cut(s) 1023, 1055
Bbv12I GWGCWC 2 cut(s) 128, 1112
BccI CCATC 1 cut(s) 1024
BceAI ACGGC 1 cut(s) 30
BciVI GTATCC 1 cut(s) 461
BclI TGATCA 2 cut(s) 433, 778
BcnI CCSGG 1 cut(s) 335
BcoDI GTCTC 3 cut(s) 94, 523, 1134
BfaI CTAG 2 cut(s) 732, 741
BfmI CTRYAG 6 cut(s) 498, 538, 642, 835, 1040, 1162
BfrI CTTAAG 1 cut(s) 1069
BfuAI ACCTGC 1 cut(s) 505
BfuI GTATCC 1 cut(s) 461
BglII AGATCT 1 cut(s) 350
BlpI GCTNAGC 1 cut(s) 693
BmcAI AGTACT 2 cut(s) 736, 892
Bme1390I CCNGG 1 cut(s) 335
BmeT110I CYCGRG 3 cut(s) 606, 617, 1000
BmrFI CCNGG 1 cut(s) 335
BmsI GCATC 2 cut(s) 859, 1074
BplI GAGNNNNNCTC 2 cut(s) 600, 632
BpmI CTGGAG 1 cut(s) 789
Bpu1102I GCTNAGC 1 cut(s) 693
Bpu14I TTCGAA 4 cut(s) 168, 193, 281, 627
BpuEI CTTGAG 2 cut(s) 328, 501
BpuMI CCSGG 1 cut(s) 335
Bsa29I ATCGAT 1 cut(s) 771
BsaBI GATNNNNATC 1 cut(s) 777
BsaJI CCNNGG 5 cut(s) 66, 112, 372, 560, 999
BsaWI WCCGGW 1 cut(s) 128
BsaXI ACNNNNNCTCC 2 cut(s) 299, 329
Bsc4I CCNNNNNNNGG 2 cut(s) 1094, 1152
Bse8I GATNNNNATC 1 cut(s) 777
BseAI TCCGGA 1 cut(s) 128
BseCI ATCGAT 1 cut(s) 771
BseDI CCNNGG 5 cut(s) 66, 112, 372, 560, 999
BseGI GGATG 1 cut(s) 995
BseJI GATNNNNATC 1 cut(s) 777
BseLI CCNNNNNNNGG 2 cut(s) 1094, 1152
BseMII CTCAG 3 cut(s) 89, 404, 685
BseRI GAGGAG 4 cut(s) 317, 320, 792, 1135
BseYI CCCAGC 2 cut(s) 84, 211
Bsh1236I CGCG 2 cut(s) 24, 231
Bsh1285I CGRYCG 1 cut(s) 220
BshFI GGCC 2 cut(s) 271, 559
BshVI ATCGAT 1 cut(s) 771
BsiEI CGRYCG 1 cut(s) 220
BsiHKAI GWGCWC 2 cut(s) 128, 1112
BsiHKCI CYCGRG 3 cut(s) 606, 617, 1000
BsiSI CCGG 3 cut(s) 129, 335, 962
BslFI GGGAC 1 cut(s) 634
BslI CCNNNNNNNGG 2 cut(s) 1094, 1152
BsmAI GTCTC 3 cut(s) 94, 523, 1134
BsmFI GGGAC 1 cut(s) 634
BsnI GGCC 2 cut(s) 271, 559
BsoBI CYCGRG 3 cut(s) 606, 617, 1000
Bsp119I TTCGAA 4 cut(s) 168, 193, 281, 627
Bsp1286I GDGCHC 3 cut(s) 128, 1007, 1112
Bsp13I TCCGGA 1 cut(s) 128
Bsp1720I GCTNAGC 1 cut(s) 693
Bsp19I CCATGG 3 cut(s) 66, 372, 560
BspACI CCGC 7 cut(s) 109, 121, 231, 422, 553, 593, 1095
BspANI GGCC 2 cut(s) 271, 559
BspCNI CTCAG 3 cut(s) 90, 403, 684
BspDI ATCGAT 1 cut(s) 771
BspEI TCCGGA 1 cut(s) 128
BspFNI CGCG 2 cut(s) 24, 231
BspHI TCATGA 3 cut(s) 136, 409, 775
BspMAI CTGCAG 2 cut(s) 502, 646
BspMI ACCTGC 1 cut(s) 505
BspPI GGATC 5 cut(s) 251, 258, 584, 731, 1112
BspQI GCTCTTC 1 cut(s) 1012
BspT104I TTCGAA 4 cut(s) 168, 193, 281, 627
BspTI CTTAAG 1 cut(s) 1069
BssECI CCNNGG 5 cut(s) 66, 112, 372, 560, 999
BssT1I CCWWGG 3 cut(s) 66, 372, 560
Bst4CI ACNGT 1 cut(s) 61
Bst6I CTCTTC 2 cut(s) 260, 1012
BstAFI CTTAAG 1 cut(s) 1069
BstAPI GCANNNNNTGC 2 cut(s) 641, 650
BstBI TTCGAA 4 cut(s) 168, 193, 281, 627
BstDEI CTNAG 5 cut(s) 98, 390, 671, 693, 1128
BstDSI CCRYGG 4 cut(s) 66, 112, 372, 560
BstENI CCTNNNNNAGG 1 cut(s) 1150
BstF5I GGATG 1 cut(s) 995
BstFNI CGCG 2 cut(s) 24, 231
BstHHI GCGC 3 cut(s) 26, 231, 814
BstMAI GTCTC 3 cut(s) 94, 523, 1134
BstMCI CGRYCG 1 cut(s) 220
BstMWI GCNNNNNNNGC 3 cut(s) 641, 650, 806
BstSCI CCNGG 1 cut(s) 333
BstSFI CTRYAG 6 cut(s) 498, 538, 642, 835, 1040, 1162
BstUI CGCG 2 cut(s) 24, 231
BstX2I RGATCY 2 cut(s) 350, 1117
BstYI RGATCY 2 cut(s) 350, 1117
Bsu15I ATCGAT 1 cut(s) 771
BsuI GTATCC 1 cut(s) 461
BsuRI GGCC 2 cut(s) 271, 559
BsuTUI ATCGAT 1 cut(s) 771
BtgI CCRYGG 4 cut(s) 66, 112, 372, 560
BtsCI GGATG 1 cut(s) 995
BveI ACCTGC 1 cut(s) 505
CaiI CAGNNNCTG 1 cut(s) 641
CciI TCATGA 3 cut(s) 136, 409, 775
CfoI GCGC 3 cut(s) 26, 231, 814
ClaI ATCGAT 1 cut(s) 771
CseI GACGC 1 cut(s) 41
Csp6I GTAC 6 cut(s) 62, 735, 891, 974, 1034, 1132
CviQI GTAC 6 cut(s) 62, 735, 891, 974, 1034, 1132
DdeI CTNAG 5 cut(s) 98, 390, 671, 693, 1128
Eam1104I CTCTTC 2 cut(s) 260, 1012
EarI CTCTTC 2 cut(s) 260, 1012
EciI GGCGGA 1 cut(s) 568
Ecl136II GAGCTC 2 cut(s) 126, 1110
Eco130I CCWWGG 3 cut(s) 66, 372, 560
Eco24I GRGCYC 3 cut(s) 128, 1007, 1112
Eco53kI GAGCTC 2 cut(s) 126, 1110
Eco88I CYCGRG 3 cut(s) 606, 617, 1000
EcoICRI GAGCTC 2 cut(s) 126, 1110
EcoNI CCTNNNNNAGG 1 cut(s) 1150
EcoRI GAATTC 1 cut(s) 170
EcoT14I CCWWGG 3 cut(s) 66, 372, 560
EcoT38I GRGCYC 3 cut(s) 128, 1007, 1112
ErhI CCWWGG 3 cut(s) 66, 372, 560
FalI AAGNNNNNCTT 2 cut(s) 686, 718
FaqI GGGAC 1 cut(s) 634
FauI CCCGC 2 cut(s) 224, 429
FbaI TGATCA 2 cut(s) 433, 778
FblI GTMKAC 1 cut(s) 789
FokI GGATG 1 cut(s) 982
FriOI GRGCYC 3 cut(s) 128, 1007, 1112
FspBI CTAG 2 cut(s) 732, 741
GlaI GCGC 3 cut(s) 25, 230, 813
GsaI CCCAGC 2 cut(s) 88, 215
GsuI CTGGAG 1 cut(s) 789
HaeIII GGCC 2 cut(s) 271, 559
HapII CCGG 3 cut(s) 129, 335, 962
HgaI GACGC 1 cut(s) 41
HhaI GCGC 3 cut(s) 26, 231, 814
Hin6I GCGC 3 cut(s) 24, 229, 812
HinP1I GCGC 3 cut(s) 24, 229, 812
HindIII AAGCTT 1 cut(s) 695
HinfI GANTC 6 cut(s) 80, 90, 287, 583, 629, 1142
HpaII CCGG 3 cut(s) 129, 335, 962
HphI GGTGA 3 cut(s) 65, 406, 1169
Hpy166II GTNNAC 3 cut(s) 20, 340, 790
Hpy188I TCNGA 6 cut(s) 99, 238, 255, 292, 1080, 1117
Hpy188III TCNNGA 7 cut(s) 129, 137, 410, 587, 619, 776, 1028
Hpy8I GTNNAC 3 cut(s) 20, 340, 790
HpyAV CCTTC 2 cut(s) 288, 592
HpyCH4III ACNGT 1 cut(s) 61
HpyCH4V TGCA 5 cut(s) 401, 500, 644, 758, 872
HpyF10VI GCNNNNNNNGC 3 cut(s) 641, 650, 806
HpyF3I CTNAG 5 cut(s) 98, 390, 671, 693, 1128
HspAI GCGC 3 cut(s) 24, 229, 812
Kpn2I TCCGGA 1 cut(s) 128
Ksp22I TGATCA 2 cut(s) 433, 778
LguI GCTCTTC 1 cut(s) 1012
LmnI GCTCC 4 cut(s) 13, 123, 131, 805
LweI GCATC 2 cut(s) 859, 1074
MaeI CTAG 2 cut(s) 732, 741
MaeIII GTNAC 2 cut(s) 899, 1157
MboII GAAGA 8 cut(s) 277, 307, 322, 978, 981, 999, 1064, 1095
MflI RGATCY 2 cut(s) 350, 1117
MhlI GDGCHC 3 cut(s) 128, 1007, 1112
MluCI AATT 8 cut(s) 164, 170, 189, 364, 462, 793, 856, 954
MlyI GAGTC 2 cut(s) 84, 1136
MmeI TCCRAC 1 cut(s) 216
MroI TCCGGA 1 cut(s) 128
MroXI GAANNNNTTC 1 cut(s) 714
MseI TTAA 4 cut(s) 699, 855, 969, 1070
MspA1I CMGCKG 2 cut(s) 121, 809
MspCI CTTAAG 1 cut(s) 1069
MspI CCGG 3 cut(s) 129, 335, 962
MspR9I CCNGG 1 cut(s) 335
MvnI CGCG 2 cut(s) 24, 231
MwoI GCNNNNNNNGC 3 cut(s) 641, 650, 806
NciI CCSGG 1 cut(s) 335
NcoI CCATGG 3 cut(s) 66, 372, 560
NmeAIII GCCGAG 3 cut(s) 133, 158, 604
NmuCI GTSAC 1 cut(s) 1157
NspV TTCGAA 4 cut(s) 168, 193, 281, 627
PaeR7I CTCGAG 1 cut(s) 606
PagI TCATGA 3 cut(s) 136, 409, 775
PciSI GCTCTTC 1 cut(s) 1012
PcsI WCGNNNNNNNCGW 1 cut(s) 624
PdmI GAANNNNTTC 1 cut(s) 714
PfeI GAWTC 4 cut(s) 80, 287, 583, 629
Ple19I CGATCG 1 cut(s) 220
PleI GAGTC 2 cut(s) 84, 1136
PpsI GAGTC 2 cut(s) 84, 1136
PshBI ATTAAT 2 cut(s) 855, 969
Psp124BI GAGCTC 2 cut(s) 128, 1112
PspFI CCCAGC 2 cut(s) 84, 211
PspXI VCTCGAGB 1 cut(s) 606
PstI CTGCAG 2 cut(s) 502, 646
PstNI CAGNNNCTG 1 cut(s) 641
PsuI RGATCY 2 cut(s) 350, 1117
PvuI CGATCG 1 cut(s) 220
PvuII CAGCTG 1 cut(s) 809
RsaI GTAC 6 cut(s) 63, 736, 892, 975, 1035, 1133
RsaNI GTAC 6 cut(s) 62, 735, 891, 974, 1034, 1132
SacI GAGCTC 2 cut(s) 128, 1112
SapI GCTCTTC 1 cut(s) 1012
SaqAI TTAA 4 cut(s) 699, 855, 969, 1070
ScaI AGTACT 2 cut(s) 736, 892
SchI GAGTC 2 cut(s) 84, 1136
ScrFI CCNGG 1 cut(s) 335
SduI GDGCHC 3 cut(s) 128, 1007, 1112
SfaNI GCATC 2 cut(s) 859, 1074
SfcI CTRYAG 6 cut(s) 498, 538, 642, 835, 1040, 1162
Sfr274I CTCGAG 1 cut(s) 606
SfuI TTCGAA 4 cut(s) 168, 193, 281, 627
SlaI CTCGAG 1 cut(s) 606
SmlI CTYRAG 4 cut(s) 343, 516, 606, 1069
SmoI CTYRAG 4 cut(s) 343, 516, 606, 1069
Sse9I AATT 8 cut(s) 164, 170, 189, 364, 462, 793, 856, 954
SsiI CCGC 7 cut(s) 109, 121, 231, 422, 553, 593, 1095
SspMI CTAG 2 cut(s) 732, 741
SstI GAGCTC 2 cut(s) 128, 1112
StyD4I CCNGG 1 cut(s) 333
StyI CCWWGG 3 cut(s) 66, 372, 560
TaaI ACNGT 1 cut(s) 61
TaqI TCGA 8 cut(s) 168, 193, 281, 322, 607, 627, 771, 1106
TasI AATT 8 cut(s) 164, 170, 189, 364, 462, 793, 856, 954
TatI WGTACW 3 cut(s) 734, 890, 1131
TfiI GAWTC 4 cut(s) 80, 287, 583, 629
Tru1I TTAA 4 cut(s) 699, 855, 969, 1070
Tru9I TTAA 4 cut(s) 699, 855, 969, 1070
TseFI GTSAC 1 cut(s) 1157
Tsp45I GTSAC 1 cut(s) 1157
TspDTI ATGAA 5 cut(s) 300, 426, 434, 675, 1089
TspGWI ACGGA 1 cut(s) 957
Vha464I CTTAAG 1 cut(s) 1069
VspI ATTAAT 2 cut(s) 855, 969
XagI CCTNNNNNAGG 1 cut(s) 1150
XapI RAATTY 1 cut(s) 170
XcmI CCANNNNNNNNNTGG 1 cut(s) 567
XhoI CTCGAG 1 cut(s) 606
XmiI GTMKAC 1 cut(s) 789
XmnI GAANNNNTTC 1 cut(s) 714
XspI CTAG 2 cut(s) 732, 741
ZrmI AGTACT 2 cut(s) 736, 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.