Rmu_sc0001674.1_g000008

CCR4-NOT transcription complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001674.1
OG0005282
Physical Location & Seq
Forward (+)
33536 .. 47784
14249 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5316 bp
atgctcaagttttcgcagaccactgcgaaccagattcggttcctcctgcaaagcctcaatgacgccaactcggactctgttcaccgagagcttactcagtttattgaatatggagttgagggaagcgttttgctgctccaaacctgcctggatcatctcaatcattacagaacagatacaaagaatgtggcactggaacgggttcttgcctcaattttcaaatatctcatcgaccaaccaaatttcagtactatgttttgtgaatcactaagaaatacagaggtcaatgaaggaattgtggagaatttctcgagtgtattgcaactgtctgtacctgagaaaattggcattggtcttgctttgtcagattctgaaaacctagacaccagaatatgtggcaaaaacttctgtatggctcagattgagaaattgtgtgaaactcctgttgacaggatctcttctgagaaaattcagaatattgttatgttccttcagcggtctgagggtctttccaagcatgtagattcgtttatgcagatattatctttactgcagctgaaagatgatgaaaactcttttgttttaagtccattgctttcagatgagcttcgtgatgccaattttttgaggaacatggacttgttccgtgaaagtggagaaaatgattttgacgctattttagcagaaatggagaaggagatgaatatgggagatattatgaaggaattaggttatggatgcacagttgattcatctcagtgcaaagagattttatcccttttcttacccctcaatgagattactatctccaaaatacttggcacgattgcctgcacccatgctggtcttgaggataatcagaatacattttcaaccttccggttggcgcttggttatagtacttcatctgatttgcctatgctaaacacctggaacattgatgtccttgtggatacgatcaatcaacttgctccagacaccaattggataagtgttattgaaaatttggatcatgaggggttctacattcctaatcaggaggcattctcttttttcatgactgtatatcagcatgtgtgtcaggagccattccccctacatgccatctgtgggtctgtttggaagaatactgagggacagctatcttttctcaaacatgctgtttcatcaccacctgaagtatttaattttgctcactctgttaggcagctgccctatatggatacggtgaatggccacaagctacaacttggacatacaaatcatgcatggttgtgtctagaccttttggatgtactttgtcacctagcagagaggggtcatgctctttctgttcgatcgatacttgagtatcctcttcaacactgtcctgaagtcctgcttcttgggatgacacacattaatactccttacaacctgctgcaatatgaagtgtctttcattgttttccccatgatagtaaaaaatgtcatgggtaatggcatgattgttgacctctggcatactaacccctctttggtgctgcggggattcgtggatgcctacaatagcgatccagatagcatgactagaatattggatatctgtcaagagcttaagattttgtcgtccgtcatggagatgattccttctccctttagtatcagactggcggcccttgctttcagaaaagagcttgtagaactcgagaagtggttgagtagtaatttgaatacatataaagatactttctttgaggagtgcctcaagttcctgaaggagattcaagctggagggacccaagatttctcaaccagacctttccaacattctggtgctgtctcaactctctatggggacactactgctacattctcgaaggttcttaaagctcatgtcgcccagattacttctagccaccttactgaggagatggaaaaactctctctattaattatggattctaatccaaggctgcagaatggtgggcctacagagtcatcaacagatggatatgcagatgacattgaagctgaagccaactcttacttccatcaaatgttttctggtcagttgacaattgaatcaatggtccaaatgcttgcccgtttcaaagaatcttctgtgcaaagggaacaatccatttttgagtgcatgattgctaatctgtttgaagagtacagattctttccgaaatatcctgaaagacagctcaaaattgctgcaattttatttgggtctgtcatcaagcagcagcttgtaactcatctgactctagggattgctctgcgtggtgttttagatgcattgcgcaaacctgcggattcaaaaatgtttgtatttggaacaaaggctctggagcagtttgtggagcgtctgattgaatggccacagtactgcaatcacattcttcagatatcccatttgcgcaatactcattcagagctggtggtattcattgaacaggctcttgccaggatttcatctggtcattcagattcagatggaagtaaccatgcttctgccgttcatcatcacggtcccagtcaagttacctcaggaaatgtggagttaaatggatcaagcatcttacaaactgggcagacactatctgctccgctccagctccatcagagacatgaaagctcttttgatgaccgtcacaagtcctcaactccatctaatgaggtaaagccacttttatcttctgcgacgcaaccggcagttgttcctgttagtgacgctccgagtattcagaagttacaaaatgcagttagtgctccatcaatgttgtcttctgcttcccctggttttgttcgtccgtcacgaggagctacctctactaggtttggctctgctttgagcattgaaacattggttgcagcagctgagaaaagggatgctcccgttgaggctccagcagagattcaggacaaagtattatttatagttaataatatatcacttgctaatattgaagttaagtcgaaggagttttctgagatactgaaagagcaatactatccctggtttgcgcagtatatggttatgaaaagggcaagcattgagcccaattttcatgacttgtacctgaaatttctggacaaacttaattcgaaggctttgaataaagagattgttcaagccacgtatgagaactgcaaggttcttctagggtctgagcttataaaatcaagctcagaggagcgttcattgctaaaaaatttaggtagctggcttgggaagcttactattggaaggaatcaagtcttaagggctcgtgaaattgatcccaaatctttgatcatagaggcatatgagaaagggttgatgattgctgtcataccttttacttcaaagattttggaaccatgtcagaatagcctcgcttatcaaccaccgaatccctggacaatgggtattcttggattacttgctgagatctattccatgccaaacttgaagatgaacctaagcttcattttggaaccatgtcagaatagcctcgcgtatcaaccaccgaatccctggacaatgggtattcttggattacttgctgagatctattccatgccaaacttgaagatgaacctgaagtttgatatagaggttctattcaagaatctaggagtggatttgaaggagataacaccaacttctcttctcaaggatcgcaacagagaacttgaagggaatccagatttctccaacaaagatgttggagcatctcaaccacagattgctgaagttaaatctggaattatttctccactaaaccaagttgagctaccacttgatgttgctccatctaattctgggagccacacacatctgttatctcagtatgctaccttgatggaggatgacaagcttgctttggggttatctgatcagatcacttctgctcaaggactgttacaagctactccatcacagtccccattttctgttagtcagcatcccacagcaatacctaacattggaacccacgtaattatcaaccagaagctcagtgccttgggggggctgcacgtgcatttccaaagagtggttccaattgctatggatagagctatcaaagaaattgtctctggtattgttcagcgcagtgtttctatagctacccagacaactaaggagcttgttttaaaggactatgccatggaatcagatgacacaagaatttttactgcagcgcacttgatggtcgccagtttggcagggagtctagctcatgtcacatgcaaggaacccttgcgcagttcaatattaagtcagctaagaaattctcttcagggattaaacattgcaagtgatcttctagaacaagctgttcaacttgtaaccaatgataatctggatcttggctgtgcagtcattgagcaggctgctacagataaggcggtagcaacaattgacggggaaataggtccgcagctttcagtaagaagaaagagagaaggtgttggtgcaacctattttgatgcaaacatgtatccacaaggttctatgggtgttgtgccagaagctctccgccctaaacctggtcatttgtccctttctcagcaacgagtttatgaggattttgttcggcttccctggcaaaaccaatctattcaaagttcgcacgttctgccagttgctaccacagcttcatttggtagtactggtctcactagcacatatggttcagcatccggacaacttaacacgggctactcaccaggacctggaagcaaatttgaagctgtttctcgtccattagatgaagcgatggaatccaattctgccctccatcttaggttggtgttcatagaatctggagtgtcctcacagccaccaccttcacctgctgtaactgaacgtcttggaagtctctcagaaccttccctcaccacaggggctgcattggaaaaataccagattgttgcacagaagttggaagctctggtgactaacgatgctagagaatcagaaatccagggagtcattggtgaagtgcctgatattatactcagatgtgttagtcgagatgaggccgccttggctgtggctcaaaaggttttcaagggtttgtacgagaatgcaacaaaccagattcatgttggcgcacatctagcaatgctgactgcgattcgtgatgtttgcaagcttgtctttacgcagctcaatagttgggtatgcactattacactggcagatattgtttacgcagtccggccctcccctttgaatcaagggactcgatcaggtatgagatacacacctatacctgctacgtgtaggttttatgctgatgcaaatgtgaaagctttagtctataattgcttctgctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CNOT1_CAF1_bind CNOT1_HEAT CNOT1_TTP_bind DUF3819 Not1

Protein Analysis

1771

Amino Acids

196.09

Weight (kDa)

5.46

Isoelectric Point (pI)

42.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNOT1_HEAT PF16418 482 - 623 1.5e-33 CCR4-NOT transcription complex subunit 1 HEAT repeat
CNOT1_TTP_bind PF16417 659 - 820 1.8e-67 CCR4-NOT transcription complex subunit 1 TTP binding domain
CNOT1_CAF1_bind PF16415 964 - 1155 4.8e-78 CCR4-NOT transcription complex subunit 1 CAF1-binding domain
CNOT1_CAF1_bind PF16415 1154 - 1221 6.6e-23 CCR4-NOT transcription complex subunit 1 CAF1-binding domain
DUF3819 PF12842 1339 - 1478 5.5e-48 CCR4-Not complex, Not1 subunit, domain of unknown function DUF3819
ARM_Cnot1 PF25097 1639 - 1723 4.7e-13 Cnot1 ARM repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3167
AarI CACCTGC 1 cut(s) 4837
Acc16I TGCGCA 4 cut(s) 2291, 2408, 3015, 4238
Acc36I ACCTGC 5 cut(s) 152, 1425, 2305, 4837, 5260
AccB7I CCANNNNNTGG 2 cut(s) 4027, 4709
AccBSI CCGCTC 1 cut(s) 2599
AccII CGCG 1 cut(s) 3488
AccIII TCCGGA 1 cut(s) 4676
AciI CCGC 9 cut(s) 496, 1525, 1652, 2300, 2597, 4382, 4412, 4513, 5019
AclWI GGATC 8 cut(s) 159, 461, 1017, 1547, 2566, 3263, 3657, 4347
AcoI YGGCCR 2 cut(s) 1234, 2366
AcsI RAATTY 9 cut(s) 241, 304, 468, 1003, 3074, 3202, 4161, 4264, 4718
AcuI CTGAAG 9 cut(s) 476, 1197, 1392, 1775, 2034, 2375, 3593, 3744, 4256
AcvI CACGTG 1 cut(s) 4012
AcyI GRCGYC 1 cut(s) 63
AfaI GTAC 9 cut(s) 250, 333, 901, 1296, 2159, 2375, 3068, 4645, 5057
AflII CTTAAG 2 cut(s) 1595, 3250
AflIII ACRYGT 2 cut(s) 4470, 5258
AjuI GAANNNNNNNTTGG 4 cut(s) 2012, 2044, 4014, 4046
Alw21I GWGCWC 1 cut(s) 2761
Alw26I GTCTC 5 cut(s) 1825, 2608, 4072, 4655, 4859
AlwI GGATC 8 cut(s) 159, 461, 1017, 1547, 2566, 3263, 3657, 4347
AlwNI CAGNNNCTG 4 cut(s) 371, 2867, 4709, 4883
Ama87I CYCGRG 2 cut(s) 310, 1685
Aor13HI TCCGGA 1 cut(s) 4676
AoxI GGCC 6 cut(s) 1234, 1653, 1967, 2366, 5016, 5198
ApoI RAATTY 9 cut(s) 241, 304, 468, 1003, 3074, 3202, 4161, 4264, 4718
ArsI GACNNNNNNTTYG 2 cut(s) 4050, 4082
AseI ATTAAT 2 cut(s) 1401, 1931
Asp700I GAANNNNTTC 2 cut(s) 201, 3742
AspLEI GCGC 8 cut(s) 889, 2292, 2409, 3016, 4086, 4177, 4239, 5090
AspS9I GGNCC 8 cut(s) 1654, 1776, 1967, 2071, 2519, 4409, 4706, 5199
AsuHPI GGTGA 9 cut(s) 74, 1161, 1240, 1295, 4692, 4818, 4864, 4942, 4985
AsuII TTCGAA 1 cut(s) 3095
AvaI CYCGRG 2 cut(s) 310, 1685
AvaII GGWCC 5 cut(s) 1776, 2071, 2519, 4409, 4706
AxyI CCTNAGG 1 cut(s) 2536
BaeI ACNNNNGTAYC 4 cut(s) 1334, 1334, 1367, 1367
BalI TGGCCA 2 cut(s) 1236, 2368
BanII GRGCYC 2 cut(s) 3051, 3259
BarI GAAGNNNNNNTAC 2 cut(s) 2482, 2514
BauI CACGAG 2 cut(s) 2805, 3258
BbrPI CACGTG 1 cut(s) 4012
BbsI GAAGAC 1 cut(s) 2766
Bbv12I GWGCWC 1 cut(s) 2761
BceAI ACGGC 1 cut(s) 2489
BcgI CGANNNNNNTGC 2 cut(s) 301, 335
BciVI GTATCC 4 cut(s) 946, 1216, 1362, 4485
BclI TGATCA 2 cut(s) 3282, 3868
BcoDI GTCTC 5 cut(s) 1825, 2608, 4072, 4655, 4859
BfmI CTRYAG 6 cut(s) 551, 1955, 1971, 4095, 4170, 4371
BfoI RGCGCY 1 cut(s) 890
BfrI CTTAAG 2 cut(s) 1595, 3250
BfuAI ACCTGC 5 cut(s) 152, 1425, 2305, 4837, 5260
BfuI GTATCC 4 cut(s) 946, 1216, 1362, 4485
BglI GCCNNNNNGGC 2 cut(s) 4196, 5024
BglII AGATCT 2 cut(s) 3420, 3540
BmcAI AGTACT 4 cut(s) 250, 901, 2375, 4645
Bme18I GGWCC 5 cut(s) 1776, 2071, 2519, 4409, 4706
BmeT110I CYCGRG 2 cut(s) 310, 1685
BmgT120I GGNCC 8 cut(s) 1654, 1776, 1967, 2071, 2519, 4409, 4706, 5199
BmrI ACTGGG 2 cut(s) 2517, 2586
BmuI ACTGGG 2 cut(s) 2517, 2586
BpiI GAAGAC 1 cut(s) 2766
BplI GAGNNNNNCTC 6 cut(s) 293, 325, 1031, 1063, 4195, 4227
BpmI CTGGAG 6 cut(s) 957, 1791, 2357, 2585, 2880, 4821
Bpu10I CCTNAGC 1 cut(s) 3452
Bpu14I TTCGAA 1 cut(s) 3095
BpuEI CTTGAG 5 cut(s) 869, 1367, 1730, 3629, 3870
Bsa29I ATCGAT 1 cut(s) 1340
BsaAI YACGTR 4 cut(s) 3129, 3970, 4012, 5259
BsaBI GATNNNNATC 2 cut(s) 803, 1944
BsaHI GRCGYC 1 cut(s) 63
BsaI GGTCTC 1 cut(s) 4655
BsaWI WCCGGW 2 cut(s) 879, 4676
Bse118I RCCGGY 1 cut(s) 2698
Bse1I ACTGG 8 cut(s) 198, 1653, 2523, 2581, 4191, 4616, 4651, 5178
Bse21I CCTNAGG 1 cut(s) 2536
Bse3DI GCAATG 5 cut(s) 590, 2285, 3191, 4284, 5106
Bse8I GATNNNNATC 2 cut(s) 803, 1944
BseAI TCCGGA 1 cut(s) 4676
BseCI ATCGAT 1 cut(s) 1340
BseGI GGATG 8 cut(s) 743, 1297, 1395, 1543, 2884, 3847, 3937, 4673
BseJI GATNNNNATC 2 cut(s) 803, 1944
BseMI GCAATG 5 cut(s) 590, 2285, 3191, 4284, 5106
BseNI ACTGG 8 cut(s) 198, 1653, 2523, 2581, 4191, 4616, 4651, 5178
BseRI GAGGAG 4 cut(s) 1751, 1922, 2823, 3197
BsgI GTGCAG 3 cut(s) 817, 3992, 4371
Bsh1236I CGCG 1 cut(s) 3488
Bsh1285I CGRYCG 1 cut(s) 1340
BshFI GGCC 6 cut(s) 1236, 1655, 1969, 2368, 5018, 5200
BshVI ATCGAT 1 cut(s) 1340
BsiEI CGRYCG 1 cut(s) 1340
BsiHKAI GWGCWC 1 cut(s) 2761
BsiHKCI CYCGRG 2 cut(s) 310, 1685
BsiSI CCGG 4 cut(s) 880, 2699, 4677, 5197
BslFI GGGAC 7 cut(s) 1149, 1789, 1850, 2505, 3901, 4519, 5233
BsmAI GTCTC 5 cut(s) 1825, 2608, 4072, 4655, 4859
BsmFI GGGAC 7 cut(s) 1149, 1789, 1850, 2505, 3901, 4519, 5233
BsmI GAATGC 2 cut(s) 1043, 5068
BsnI GGCC 6 cut(s) 1236, 1655, 1969, 2368, 5018, 5200
Bso31I GGTCTC 1 cut(s) 4655
BsoBI CYCGRG 2 cut(s) 310, 1685
Bsp119I TTCGAA 1 cut(s) 3095
Bsp1286I GDGCHC 3 cut(s) 2761, 3051, 3259
Bsp13I TCCGGA 1 cut(s) 4676
Bsp19I CCATGG 1 cut(s) 4140
BspACI CCGC 9 cut(s) 496, 1525, 1652, 2300, 2597, 4382, 4412, 4513, 5019
BspANI GGCC 6 cut(s) 1236, 1655, 1969, 2368, 5018, 5200
BspDI ATCGAT 1 cut(s) 1340
BspEI TCCGGA 1 cut(s) 4676
BspFNI CGCG 1 cut(s) 3488
BspHI TCATGA 3 cut(s) 1012, 1056, 3058
BspMAI CTGCAG 3 cut(s) 555, 1959, 4174
BspMI ACCTGC 5 cut(s) 152, 1425, 2305, 4837, 5260
BspPI GGATC 8 cut(s) 159, 461, 1017, 1547, 2566, 3263, 3657, 4347
BspT104I TTCGAA 1 cut(s) 3095
BspTI CTTAAG 2 cut(s) 1595, 3250
BspTNI GGTCTC 1 cut(s) 4655
BsrBI CCGCTC 1 cut(s) 2599
BsrDI GCAATG 5 cut(s) 590, 2285, 3191, 4284, 5106
BsrFI RCCGGY 1 cut(s) 2698
BsrI ACTGG 8 cut(s) 198, 1653, 2523, 2581, 4191, 4616, 4651, 5178
BssAI RCCGGY 1 cut(s) 2698
BssNI GRCGYC 1 cut(s) 63
BssSI CACGAG 2 cut(s) 2805, 3258
BssT1I CCWWGG 4 cut(s) 1949, 3996, 4140, 5022
Bst2BI CACGAG 2 cut(s) 2805, 3258
Bst6I CTCTTC 5 cut(s) 463, 1362, 2148, 3645, 4275
BstACI GRCGYC 1 cut(s) 63
BstAFI CTTAAG 2 cut(s) 1595, 3250
BstAPI GCANNNNNTGC 2 cut(s) 2756, 4612
BstBAI YACGTR 4 cut(s) 3129, 3970, 4012, 5259
BstBI TTCGAA 1 cut(s) 3095
BstC8I GCNNGC 7 cut(s) 832, 2082, 3040, 3215, 3852, 4365, 5129
BstDSI CCRYGG 1 cut(s) 4140
BstENI CCTNNNNNAGG 2 cut(s) 1904, 2821
BstF5I GGATG 8 cut(s) 743, 1297, 1395, 1543, 2884, 3847, 3937, 4673
BstFNI CGCG 1 cut(s) 3488
BstH2I RGCGCY 1 cut(s) 890
BstHHI GCGC 8 cut(s) 889, 2292, 2409, 3016, 4086, 4177, 4239, 5090
BstMAI GTCTC 5 cut(s) 1825, 2608, 4072, 4655, 4859
BstMCI CGRYCG 1 cut(s) 1340
BstNSI RCATGY 6 cut(s) 521, 1076, 1103, 1160, 4224, 4474
BstSFI CTRYAG 6 cut(s) 551, 1955, 1971, 4095, 4170, 4371
BstUI CGCG 1 cut(s) 3488
BstV2I GAAGAC 1 cut(s) 2766
BstX2I RGATCY 4 cut(s) 453, 3420, 3540, 4339
BstXI CCANNNNNNTGG 1 cut(s) 1811
BstYI RGATCY 4 cut(s) 453, 3420, 3540, 4339
Bsu15I ATCGAT 1 cut(s) 1340
Bsu36I CCTNAGG 1 cut(s) 2536
BsuI GTATCC 4 cut(s) 946, 1216, 1362, 4485
BsuRI GGCC 6 cut(s) 1236, 1655, 1969, 2368, 5018, 5200
BsuTUI ATCGAT 1 cut(s) 1340
BtgI CCRYGG 1 cut(s) 4140
BtgZI GCGATG 1 cut(s) 4766
BtsCI GGATG 8 cut(s) 743, 1297, 1395, 1543, 2884, 3847, 3937, 4673
BtsI GCAGTG 2 cut(s) 21, 4093
BtsIMutI CAGTG 7 cut(s) 21, 191, 764, 1363, 3997, 4093, 5171
BveI ACCTGC 5 cut(s) 152, 1425, 2305, 4837, 5260
Cac8I GCNNGC 7 cut(s) 832, 2082, 3040, 3215, 3852, 4365, 5129
CaiI CAGNNNCTG 4 cut(s) 371, 2867, 4709, 4883
CciI TCATGA 3 cut(s) 1012, 1056, 3058
CfoI GCGC 8 cut(s) 889, 2292, 2409, 3016, 4086, 4177, 4239, 5090
Cfr10I RCCGGY 1 cut(s) 2698
Cfr13I GGNCC 8 cut(s) 1654, 1776, 1967, 2071, 2519, 4409, 4706, 5199
ClaI ATCGAT 1 cut(s) 1340
CseI GACGC 5 cut(s) 71, 680, 2342, 2701, 2729
CsiI ACCWGGT 1 cut(s) 4522
Csp6I GTAC 9 cut(s) 249, 332, 900, 1295, 2158, 2374, 3067, 4644, 5056
CspCI CAANNNNNGTGG 2 cut(s) 168, 203
CviQI GTAC 9 cut(s) 249, 332, 900, 1295, 2158, 2374, 3067, 4644, 5056
DraI TTTAAA 1 cut(s) 4128
EaeI YGGCCR 2 cut(s) 1234, 2366
Eam1104I CTCTTC 5 cut(s) 463, 1362, 2148, 3645, 4275
EarI CTCTTC 5 cut(s) 463, 1362, 2148, 3645, 4275
EciI GGCGGA 1 cut(s) 4502
Eco130I CCWWGG 4 cut(s) 1949, 3996, 4140, 5022
Eco24I GRGCYC 2 cut(s) 3051, 3259
Eco31I GGTCTC 1 cut(s) 4655
Eco32I GATATC 2 cut(s) 1582, 2397
Eco47I GGWCC 5 cut(s) 1776, 2071, 2519, 4409, 4706
Eco57I CTGAAG 9 cut(s) 476, 1197, 1392, 1775, 2034, 2375, 3593, 3744, 4256
Eco72I CACGTG 1 cut(s) 4012
Eco81I CCTNAGG 1 cut(s) 2536
Eco88I CYCGRG 2 cut(s) 310, 1685
EcoNI CCTNNNNNAGG 2 cut(s) 1904, 2821
EcoO109I RGGNCCY 2 cut(s) 1776, 4706
EcoRV GATATC 2 cut(s) 1582, 2397
EcoT14I CCWWGG 4 cut(s) 1949, 3996, 4140, 5022
EcoT22I ATGCAT 2 cut(s) 1270, 2287
EcoT38I GRGCYC 2 cut(s) 3051, 3259
ErhI CCWWGG 4 cut(s) 1949, 3996, 4140, 5022
FalI AAGNNNNNCTT 6 cut(s) 1365, 1397, 4217, 4249, 5120, 5152
FaqI GGGAC 7 cut(s) 1149, 1789, 1850, 2505, 3901, 4519, 5233
FauI CCCGC 1 cut(s) 1518
FauNDI CATATG 2 cut(s) 3295, 4663
FbaI TGATCA 2 cut(s) 3282, 3868
FokI GGATG 8 cut(s) 750, 1304, 1402, 1550, 2891, 3854, 3924, 4660
FriOI GRGCYC 2 cut(s) 3051, 3259
FspI TGCGCA 4 cut(s) 2291, 2408, 3015, 4238
GlaI GCGC 8 cut(s) 888, 2291, 2408, 3015, 4085, 4176, 4238, 5089
GsuI CTGGAG 6 cut(s) 957, 1791, 2357, 2585, 2880, 4821
HaeII RGCGCY 1 cut(s) 890
HaeIII GGCC 6 cut(s) 1236, 1655, 1969, 2368, 5018, 5200
HapII CCGG 4 cut(s) 880, 2699, 4677, 5197
HgaI GACGC 5 cut(s) 71, 680, 2342, 2701, 2729
HhaI GCGC 8 cut(s) 889, 2292, 2409, 3016, 4086, 4177, 4239, 5090
Hin1I GRCGYC 1 cut(s) 63
Hin6I GCGC 8 cut(s) 887, 2290, 2407, 3014, 4084, 4175, 4237, 5088
HinP1I GCGC 8 cut(s) 887, 2290, 2407, 3014, 4084, 4175, 4237, 5088
HincII GTYRAC 3 cut(s) 448, 1492, 2055
HindII GTYRAC 3 cut(s) 448, 1492, 2055
HindIII AAGCTT 5 cut(s) 3224, 3454, 3848, 5129, 5289
HpaII CCGG 4 cut(s) 880, 2699, 4677, 5197
HphI GGTGA 9 cut(s) 74, 1161, 1240, 1295, 4692, 4818, 4864, 4942, 4985
Hpy166II GTNNAC 5 cut(s) 82, 448, 1492, 2055, 5188
Hpy8I GTNNAC 5 cut(s) 82, 448, 1492, 2055, 5188
Hpy99I CGWCG 1 cut(s) 2695
HpyCH4IV ACGT 6 cut(s) 3128, 3969, 4011, 4608, 4843, 5258
HpySE526I ACGT 6 cut(s) 3128, 3969, 4011, 4608, 4843, 5258
Hsp92I GRCGYC 1 cut(s) 63
HspAI GCGC 8 cut(s) 887, 2290, 2407, 3014, 4084, 4175, 4237, 5088
KflI GGGWCCC 1 cut(s) 1776
Kpn2I TCCGGA 1 cut(s) 4676
Ksp22I TGATCA 2 cut(s) 3282, 3868
MabI ACCWGGT 1 cut(s) 4522
MaeII ACGT 6 cut(s) 3128, 3969, 4011, 4608, 4843, 5258
MbiI CCGCTC 1 cut(s) 2599
MfeI CAATTG 4 cut(s) 982, 2058, 4035, 4392
MflI RGATCY 4 cut(s) 453, 3420, 3540, 4339
MhlI GDGCHC 3 cut(s) 2761, 3051, 3259
MlsI TGGCCA 2 cut(s) 1236, 2368
MluNI TGGCCA 2 cut(s) 1236, 2368
MlyI GAGTC 6 cut(s) 68, 1985, 2245, 4213, 4974, 5215
MmeI TCCRAC 4 cut(s) 1828, 3679, 3711, 4899
Mox20I TGGCCA 2 cut(s) 1236, 2368
Mph1103I ATGCAT 2 cut(s) 1270, 2287
MroI TCCGGA 1 cut(s) 4676
MroXI GAANNNNTTC 2 cut(s) 201, 3742
MscI TGGCCA 2 cut(s) 1236, 2368
MslI CAYNNNNRTG 4 cut(s) 757, 1273, 1619, 1812
Msp20I TGGCCA 2 cut(s) 1236, 2368
MspA1I CMGCKG 4 cut(s) 496, 556, 1210, 2867
MspCI CTTAAG 2 cut(s) 1595, 3250
MspI CCGG 4 cut(s) 880, 2699, 4677, 5197
MunI CAATTG 4 cut(s) 982, 2058, 4035, 4392
Mva1269I GAATGC 2 cut(s) 1043, 5068
MvnI CGCG 1 cut(s) 3488
NcoI CCATGG 1 cut(s) 4140
NdeI CATATG 2 cut(s) 3295, 4663
NmuCI GTSAC 6 cut(s) 1301, 2639, 2717, 2802, 4216, 4930
NsbI TGCGCA 4 cut(s) 2291, 2408, 3015, 4238
NsiI ATGCAT 2 cut(s) 1270, 2287
NspI RCATGY 6 cut(s) 521, 1076, 1103, 1160, 4224, 4474
NspV TTCGAA 1 cut(s) 3095
PaeR7I CTCGAG 2 cut(s) 310, 1685
PagI TCATGA 3 cut(s) 1012, 1056, 3058
PaqCI CACCTGC 1 cut(s) 4837
PciI ACATGT 1 cut(s) 4470
PcsI WCGNNNNNNNCGW 1 cut(s) 2803
PctI GAATGC 2 cut(s) 1043, 5068
PdmI GAANNNNTTC 2 cut(s) 201, 3742
PflMI CCANNNNNTGG 2 cut(s) 4027, 4709
Ple19I CGATCG 1 cut(s) 1340
PleI GAGTC 6 cut(s) 68, 1984, 2245, 4212, 4973, 5215
PmaCI CACGTG 1 cut(s) 4012
PmlI CACGTG 1 cut(s) 4012
PpsI GAGTC 6 cut(s) 68, 1984, 2245, 4212, 4973, 5215
Ppu21I YACGTR 4 cut(s) 3129, 3970, 4012, 5259
PpuMI RGGWCCY 2 cut(s) 1776, 4706
PscI ACATGT 1 cut(s) 4470
PshBI ATTAAT 2 cut(s) 1401, 1931
PsiI TTATAA 1 cut(s) 3167
Psp5II RGGWCCY 2 cut(s) 1776, 4706
PspCI CACGTG 1 cut(s) 4012
PspPI GGNCC 8 cut(s) 1654, 1776, 1967, 2071, 2519, 4409, 4706, 5199
PspPPI RGGWCCY 2 cut(s) 1776, 4706
PstI CTGCAG 3 cut(s) 555, 1959, 4174
PstNI CAGNNNCTG 4 cut(s) 371, 2867, 4709, 4883
PsuI RGATCY 4 cut(s) 453, 3420, 3540, 4339
PvuI CGATCG 1 cut(s) 1340
PvuII CAGCTG 3 cut(s) 556, 1210, 2867
RsaI GTAC 9 cut(s) 250, 333, 901, 1296, 2159, 2375, 3068, 4645, 5057
RsaNI GTAC 9 cut(s) 249, 332, 900, 1295, 2158, 2374, 3067, 4644, 5056
RseI CAYNNNNRTG 4 cut(s) 757, 1273, 1619, 1812
Sau96I GGNCC 8 cut(s) 1654, 1776, 1967, 2071, 2519, 4409, 4706, 5199
ScaI AGTACT 4 cut(s) 250, 901, 2375, 4645
SchI GAGTC 6 cut(s) 68, 1985, 2245, 4213, 4974, 5215
SduI GDGCHC 3 cut(s) 2761, 3051, 3259
SexAI ACCWGGT 1 cut(s) 4522
SfcI CTRYAG 6 cut(s) 551, 1955, 1971, 4095, 4170, 4371
Sfr274I CTCGAG 2 cut(s) 310, 1685
SfuI TTCGAA 1 cut(s) 3095
SinI GGWCC 5 cut(s) 1776, 2071, 2519, 4409, 4706
SlaI CTCGAG 2 cut(s) 310, 1685
SmiMI CAYNNNNRTG 4 cut(s) 757, 1273, 1619, 1812
SsiI CCGC 9 cut(s) 496, 1525, 1652, 2300, 2597, 4382, 4412, 4513, 5019
SspI AATATT 4 cut(s) 478, 1575, 2953, 4248
StyI CCWWGG 4 cut(s) 1949, 3996, 4140, 5022
TaiI ACGT 6 cut(s) 3131, 3972, 4014, 4611, 4846, 5261
TatI WGTACW 6 cut(s) 248, 899, 1294, 2157, 2373, 4643
TauI GCSGC 2 cut(s) 1655, 5021
TscAI CASTG 7 cut(s) 28, 198, 764, 1370, 3997, 4093, 5178
TseFI GTSAC 6 cut(s) 1301, 2639, 2717, 2802, 4216, 4930
Tsp45I GTSAC 6 cut(s) 1301, 2639, 2717, 2802, 4216, 4930
TspGWI ACGGA 3 cut(s) 635, 1600, 2790
TspRI CASTG 7 cut(s) 28, 198, 764, 1370, 3997, 4093, 5178
Van91I CCANNNNNTGG 2 cut(s) 4027, 4709
Vha464I CTTAAG 2 cut(s) 1595, 3250
VpaK11BI GGWCC 5 cut(s) 1776, 2071, 2519, 4409, 4706
VspI ATTAAT 2 cut(s) 1401, 1931
XagI CCTNNNNNAGG 2 cut(s) 1904, 2821
XapI RAATTY 9 cut(s) 241, 304, 468, 1003, 3074, 3202, 4161, 4264, 4718
XbaI TCTAGA 2 cut(s) 1279, 4300
XceI RCATGY 6 cut(s) 521, 1076, 1103, 1160, 4224, 4474
XcmI CCANNNNNNNNNTGG 6 cut(s) 981, 3384, 3504, 4333, 4967, 5081
XhoI CTCGAG 2 cut(s) 310, 1685
XmnI GAANNNNTTC 2 cut(s) 201, 3742
ZrmI AGTACT 4 cut(s) 250, 901, 2375, 4645
Zsp2I ATGCAT 2 cut(s) 1270, 2287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.