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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 77 |
| AccI | GTMKAC | 1 cut(s) | 240 |
| AciI | CCGC | 1 cut(s) | 75 |
| AcyI | GRCGYC | 1 cut(s) | 94 |
| AfaI | GTAC | 1 cut(s) | 125 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 297 |
| AgsI | TTSAA | 4 cut(s) | 23, 211, 218, 247 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 247, 314 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 159, 191 |
| AluBI | AGCT | 1 cut(s) | 257 |
| AluI | AGCT | 1 cut(s) | 257 |
| Alw26I | GTCTC | 2 cut(s) | 85, 367 |
| AspLEI | GCGC | 3 cut(s) | 40, 163, 282 |
| BccI | CCATC | 1 cut(s) | 20 |
| BcoDI | GTCTC | 2 cut(s) | 85, 367 |
| BfaI | CTAG | 2 cut(s) | 144, 164 |
| BfmI | CTRYAG | 1 cut(s) | 252 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 247, 314 |
| BmsI | GCATC | 1 cut(s) | 130 |
| BsaHI | GRCGYC | 1 cut(s) | 94 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 297 |
| Bse1I | ACTGG | 1 cut(s) | 314 |
| BseGI | GGATG | 1 cut(s) | 145 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 297 |
| BseMII | CTCAG | 1 cut(s) | 378 |
| BseNI | ACTGG | 1 cut(s) | 314 |
| BseRI | GAGGAG | 1 cut(s) | 188 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 297 |
| BsmAI | GTCTC | 2 cut(s) | 85, 367 |
| BsmBI | CGTCTC | 2 cut(s) | 85, 367 |
| BspACI | CCGC | 1 cut(s) | 75 |
| BspCNI | CTCAG | 1 cut(s) | 377 |
| BspQI | GCTCTTC | 1 cut(s) | 162 |
| BsrBI | CCGCTC | 1 cut(s) | 77 |
| BsrI | ACTGG | 1 cut(s) | 314 |
| BssNI | GRCGYC | 1 cut(s) | 94 |
| Bst6I | CTCTTC | 1 cut(s) | 162 |
| BstACI | GRCGYC | 1 cut(s) | 94 |
| BstDEI | CTNAG | 1 cut(s) | 364 |
| BstF5I | GGATG | 1 cut(s) | 145 |
| BstHHI | GCGC | 3 cut(s) | 40, 163, 282 |
| BstMAI | GTCTC | 2 cut(s) | 85, 367 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 169 |
| BstSFI | CTRYAG | 1 cut(s) | 252 |
| BtsCI | GGATG | 1 cut(s) | 145 |
| CfoI | GCGC | 3 cut(s) | 40, 163, 282 |
| CseI | GACGC | 1 cut(s) | 102 |
| Csp6I | GTAC | 1 cut(s) | 124 |
| CviAII | CATG | 3 cut(s) | 100, 127, 292 |
| CviJI | RGCY | 2 cut(s) | 131, 257 |
| CviKI_1 | RGCY | 2 cut(s) | 131, 257 |
| CviQI | GTAC | 1 cut(s) | 124 |
| DdeI | CTNAG | 1 cut(s) | 364 |
| DriI | GACNNNNNGTC | 2 cut(s) | 247, 314 |
| Eam1104I | CTCTTC | 1 cut(s) | 162 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 247, 314 |
| EarI | CTCTTC | 1 cut(s) | 162 |
| Esp3I | CGTCTC | 2 cut(s) | 85, 367 |
| FaeI | CATG | 3 cut(s) | 103, 130, 295 |
| FaiI | YATR | 7 cut(s) | 54, 101, 128, 154, 264, 293, 320 |
| FatI | CATG | 3 cut(s) | 99, 126, 291 |
| FblI | GTMKAC | 1 cut(s) | 240 |
| FokI | GGATG | 1 cut(s) | 152 |
| FspBI | CTAG | 2 cut(s) | 144, 164 |
| GlaI | GCGC | 3 cut(s) | 39, 162, 281 |
| HgaI | GACGC | 1 cut(s) | 102 |
| HhaI | GCGC | 3 cut(s) | 40, 163, 282 |
| Hin1I | GRCGYC | 1 cut(s) | 94 |
| Hin1II | CATG | 3 cut(s) | 103, 130, 295 |
| Hin6I | GCGC | 3 cut(s) | 38, 161, 280 |
| HinP1I | GCGC | 3 cut(s) | 38, 161, 280 |
| HincII | GTYRAC | 1 cut(s) | 241 |
| HindII | GTYRAC | 1 cut(s) | 241 |
| Hpy166II | GTNNAC | 2 cut(s) | 241, 308 |
| Hpy188I | TCNGA | 2 cut(s) | 116, 271 |
| Hpy188III | TCNNGA | 1 cut(s) | 85 |
| Hpy8I | GTNNAC | 2 cut(s) | 241, 308 |
| HpyAV | CCTTC | 1 cut(s) | 364 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 169 |
| HpyF3I | CTNAG | 1 cut(s) | 364 |
| Hsp92I | GRCGYC | 1 cut(s) | 94 |
| Hsp92II | CATG | 3 cut(s) | 103, 130, 295 |
| HspAI | GCGC | 3 cut(s) | 38, 161, 280 |
| LguI | GCTCTTC | 1 cut(s) | 162 |
| LmnI | GCTCC | 1 cut(s) | 222 |
| LpnPI | CCDG | 2 cut(s) | 48, 327 |
| LweI | GCATC | 1 cut(s) | 130 |
| MaeI | CTAG | 2 cut(s) | 144, 164 |
| MbiI | CCGCTC | 1 cut(s) | 77 |
| MboII | GAAGA | 1 cut(s) | 179 |
| MluCI | AATT | 3 cut(s) | 149, 265, 333 |
| MnlI | CCTC | 3 cut(s) | 206, 209, 293 |
| MseI | TTAA | 1 cut(s) | 48 |
| MslI | CAYNNNNRTG | 1 cut(s) | 86 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 169 |
| NlaIII | CATG | 3 cut(s) | 103, 130, 295 |
| PciSI | GCTCTTC | 1 cut(s) | 162 |
| RsaI | GTAC | 1 cut(s) | 125 |
| RsaNI | GTAC | 1 cut(s) | 124 |
| RseI | CAYNNNNRTG | 1 cut(s) | 86 |
| SalI | GTCGAC | 1 cut(s) | 239 |
| SapI | GCTCTTC | 1 cut(s) | 162 |
| SaqAI | TTAA | 1 cut(s) | 48 |
| SetI | ASST | 2 cut(s) | 259, 356 |
| SfaNI | GCATC | 1 cut(s) | 130 |
| SfcI | CTRYAG | 1 cut(s) | 252 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 86 |
| Sse9I | AATT | 3 cut(s) | 149, 265, 333 |
| SsiI | CCGC | 1 cut(s) | 75 |
| SspMI | CTAG | 2 cut(s) | 144, 164 |
| TaqI | TCGA | 1 cut(s) | 240 |
| TasI | AATT | 3 cut(s) | 149, 265, 333 |
| TatI | WGTACW | 1 cut(s) | 123 |
| Tru1I | TTAA | 1 cut(s) | 48 |
| Tru9I | TTAA | 1 cut(s) | 48 |
| XmiI | GTMKAC | 1 cut(s) | 240 |
| XspI | CTAG | 2 cut(s) | 144, 164 |