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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 112, 180 |
| AciI | CCGC | 1 cut(s) | 162 |
| AclWI | GGATC | 2 cut(s) | 88, 101 |
| AcyI | GRCGYC | 1 cut(s) | 181 |
| AfaI | GTAC | 2 cut(s) | 142, 212 |
| AgsI | TTSAA | 4 cut(s) | 87, 298, 305, 334 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 334, 401 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 246, 278 |
| AluBI | AGCT | 2 cut(s) | 344, 418 |
| AluI | AGCT | 2 cut(s) | 344, 418 |
| Alw26I | GTCTC | 1 cut(s) | 454 |
| AlwI | GGATC | 2 cut(s) | 88, 101 |
| AspLEI | GCGC | 1 cut(s) | 183 |
| BamHI | GGATCC | 1 cut(s) | 93 |
| BanI | GGYRCC | 2 cut(s) | 112, 180 |
| BauI | CACGAG | 1 cut(s) | 505 |
| BccI | CCATC | 1 cut(s) | 84 |
| BciVI | GTATCC | 1 cut(s) | 271 |
| BcoDI | GTCTC | 1 cut(s) | 454 |
| BfaI | CTAG | 2 cut(s) | 231, 251 |
| BfmI | CTRYAG | 1 cut(s) | 339 |
| BfoI | RGCGCY | 1 cut(s) | 184 |
| BfuI | GTATCC | 1 cut(s) | 271 |
| BglII | AGATCT | 1 cut(s) | 124 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 334, 401 |
| BmiI | GGNNCC | 3 cut(s) | 95, 114, 182 |
| BmsI | GCATC | 1 cut(s) | 217 |
| BsaHI | GRCGYC | 1 cut(s) | 181 |
| Bse1I | ACTGG | 1 cut(s) | 499 |
| BseGI | GGATG | 2 cut(s) | 70, 232 |
| BseMII | CTCAG | 1 cut(s) | 465 |
| BseNI | ACTGG | 1 cut(s) | 499 |
| BseRI | GAGGAG | 1 cut(s) | 275 |
| BshNI | GGYRCC | 2 cut(s) | 112, 180 |
| BsmAI | GTCTC | 1 cut(s) | 454 |
| BsmBI | CGTCTC | 1 cut(s) | 454 |
| Bsp143I | GATC | 2 cut(s) | 93, 124 |
| BspACI | CCGC | 1 cut(s) | 162 |
| BspCNI | CTCAG | 1 cut(s) | 464 |
| BspLI | GGNNCC | 3 cut(s) | 95, 114, 182 |
| BspPI | GGATC | 2 cut(s) | 88, 101 |
| BspQI | GCTCTTC | 1 cut(s) | 249 |
| BspT107I | GGYRCC | 2 cut(s) | 112, 180 |
| BsrI | ACTGG | 1 cut(s) | 499 |
| BssMI | GATC | 2 cut(s) | 93, 124 |
| BssNI | GRCGYC | 1 cut(s) | 181 |
| BssSI | CACGAG | 1 cut(s) | 505 |
| Bst2BI | CACGAG | 1 cut(s) | 505 |
| Bst6I | CTCTTC | 1 cut(s) | 249 |
| BstACI | GRCGYC | 1 cut(s) | 181 |
| BstDEI | CTNAG | 1 cut(s) | 451 |
| BstF5I | GGATG | 2 cut(s) | 70, 232 |
| BstH2I | RGCGCY | 1 cut(s) | 184 |
| BstHHI | GCGC | 1 cut(s) | 183 |
| BstKTI | GATC | 2 cut(s) | 96, 127 |
| BstMAI | GTCTC | 1 cut(s) | 454 |
| BstMBI | GATC | 2 cut(s) | 93, 124 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 469 |
| BstSFI | CTRYAG | 1 cut(s) | 339 |
| BstX2I | RGATCY | 2 cut(s) | 93, 124 |
| BstYI | RGATCY | 2 cut(s) | 93, 124 |
| BsuI | GTATCC | 1 cut(s) | 271 |
| BtsCI | GGATG | 2 cut(s) | 70, 232 |
| CfoI | GCGC | 1 cut(s) | 183 |
| Csp6I | GTAC | 2 cut(s) | 141, 211 |
| CviAII | CATG | 2 cut(s) | 187, 214 |
| CviJI | RGCY | 4 cut(s) | 10, 344, 400, 418 |
| CviKI_1 | RGCY | 4 cut(s) | 10, 344, 400, 418 |
| CviQI | GTAC | 2 cut(s) | 141, 211 |
| DdeI | CTNAG | 1 cut(s) | 451 |
| DinI | GGCGCC | 1 cut(s) | 182 |
| DpnI | GATC | 2 cut(s) | 95, 126 |
| DpnII | GATC | 2 cut(s) | 93, 124 |
| DriI | GACNNNNNGTC | 2 cut(s) | 334, 401 |
| Eam1104I | CTCTTC | 1 cut(s) | 249 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 334, 401 |
| EarI | CTCTTC | 1 cut(s) | 249 |
| EgeI | GGCGCC | 1 cut(s) | 182 |
| EheI | GGCGCC | 1 cut(s) | 182 |
| Esp3I | CGTCTC | 1 cut(s) | 454 |
| FaeI | CATG | 2 cut(s) | 190, 217 |
| FaiI | YATR | 6 cut(s) | 51, 188, 215, 241, 351, 380 |
| FatI | CATG | 2 cut(s) | 186, 213 |
| FokI | GGATG | 2 cut(s) | 77, 239 |
| FspBI | CTAG | 2 cut(s) | 231, 251 |
| GlaI | GCGC | 1 cut(s) | 182 |
| HaeII | RGCGCY | 1 cut(s) | 184 |
| HhaI | GCGC | 1 cut(s) | 183 |
| Hin1I | GRCGYC | 1 cut(s) | 181 |
| Hin1II | CATG | 2 cut(s) | 190, 217 |
| Hin6I | GCGC | 1 cut(s) | 181 |
| HinP1I | GCGC | 1 cut(s) | 181 |
| HinfI | GANTC | 1 cut(s) | 29 |
| Hpy166II | GTNNAC | 1 cut(s) | 395 |
| Hpy188I | TCNGA | 2 cut(s) | 203, 358 |
| Hpy188III | TCNNGA | 3 cut(s) | 122, 172, 505 |
| Hpy8I | GTNNAC | 1 cut(s) | 395 |
| HpyAV | CCTTC | 1 cut(s) | 451 |
| HpyCH4V | TGCA | 1 cut(s) | 248 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 469 |
| HpyF3I | CTNAG | 1 cut(s) | 451 |
| Hsp92I | GRCGYC | 1 cut(s) | 181 |
| Hsp92II | CATG | 2 cut(s) | 190, 217 |
| HspAI | GCGC | 1 cut(s) | 181 |
| KasI | GGCGCC | 1 cut(s) | 180 |
| Kzo9I | GATC | 2 cut(s) | 93, 124 |
| LguI | GCTCTTC | 1 cut(s) | 249 |
| LmnI | GCTCC | 1 cut(s) | 309 |
| LpnPI | CCDG | 4 cut(s) | 95, 135, 414, 480 |
| LweI | GCATC | 1 cut(s) | 217 |
| MaeI | CTAG | 2 cut(s) | 231, 251 |
| MaeIII | GTNAC | 2 cut(s) | 58, 403 |
| MalI | GATC | 2 cut(s) | 95, 126 |
| MboI | GATC | 2 cut(s) | 93, 124 |
| MboII | GAAGA | 1 cut(s) | 266 |
| MflI | RGATCY | 2 cut(s) | 93, 124 |
| MluCI | AATT | 5 cut(s) | 105, 236, 352, 420, 456 |
| Mly113I | GGCGCC | 1 cut(s) | 181 |
| MlyI | GAGTC | 1 cut(s) | 23 |
| MnlI | CCTC | 5 cut(s) | 49, 293, 296, 380, 501 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 469 |
| NarI | GGCGCC | 1 cut(s) | 181 |
| NdeII | GATC | 2 cut(s) | 93, 124 |
| NlaIII | CATG | 2 cut(s) | 190, 217 |
| NlaIV | GGNNCC | 3 cut(s) | 95, 114, 182 |
| NmuCI | GTSAC | 2 cut(s) | 58, 403 |
| PciSI | GCTCTTC | 1 cut(s) | 249 |
| PleI | GAGTC | 1 cut(s) | 23 |
| PluTI | GGCGCC | 1 cut(s) | 184 |
| PpsI | GAGTC | 1 cut(s) | 23 |
| PspN4I | GGNNCC | 3 cut(s) | 95, 114, 182 |
| PsuI | RGATCY | 2 cut(s) | 93, 124 |
| RsaI | GTAC | 2 cut(s) | 142, 212 |
| RsaNI | GTAC | 2 cut(s) | 141, 211 |
| SapI | GCTCTTC | 1 cut(s) | 249 |
| Sau3AI | GATC | 2 cut(s) | 93, 124 |
| SchI | GAGTC | 1 cut(s) | 23 |
| SetI | ASST | 4 cut(s) | 346, 420, 443, 512 |
| SfaNI | GCATC | 1 cut(s) | 217 |
| SfcI | CTRYAG | 1 cut(s) | 339 |
| SfoI | GGCGCC | 1 cut(s) | 182 |
| Sse9I | AATT | 5 cut(s) | 105, 236, 352, 420, 456 |
| SsiI | CCGC | 1 cut(s) | 162 |
| SspDI | GGCGCC | 1 cut(s) | 180 |
| SspMI | CTAG | 2 cut(s) | 231, 251 |
| TaqI | TCGA | 2 cut(s) | 41, 478 |
| TasI | AATT | 5 cut(s) | 105, 236, 352, 420, 456 |
| TatI | WGTACW | 1 cut(s) | 210 |
| TseFI | GTSAC | 2 cut(s) | 58, 403 |
| Tsp45I | GTSAC | 2 cut(s) | 58, 403 |
| TspDTI | ATGAA | 1 cut(s) | 503 |
| TspGWI | ACGGA | 1 cut(s) | 77 |
| XspI | CTAG | 2 cut(s) | 231, 251 |