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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AfiI | CCNNNNNNNGG | 1 cut(s) | 171 |
| AgsI | TTSAA | 3 cut(s) | 92, 121, 144 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 121, 188 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 33, 65 |
| AluBI | AGCT | 3 cut(s) | 131, 149, 205 |
| AluI | AGCT | 3 cut(s) | 131, 149, 205 |
| BccI | CCATC | 1 cut(s) | 242 |
| BfaI | CTAG | 2 cut(s) | 18, 38 |
| BfmI | CTRYAG | 1 cut(s) | 126 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 121, 188 |
| BmsI | GCATC | 1 cut(s) | 4 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 171 |
| BseGI | GGATG | 1 cut(s) | 19 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 171 |
| BseMII | CTCAG | 1 cut(s) | 252 |
| BseRI | GAGGAG | 1 cut(s) | 62 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 171 |
| BspCNI | CTCAG | 1 cut(s) | 251 |
| BspQI | GCTCTTC | 2 cut(s) | 36, 195 |
| Bst6I | CTCTTC | 2 cut(s) | 36, 195 |
| BstDEI | CTNAG | 1 cut(s) | 238 |
| BstF5I | GGATG | 1 cut(s) | 19 |
| BstSFI | CTRYAG | 1 cut(s) | 126 |
| BtsCI | GGATG | 1 cut(s) | 19 |
| CviAII | CATG | 2 cut(s) | 166, 193 |
| CviJI | RGCY | 3 cut(s) | 131, 149, 205 |
| CviKI_1 | RGCY | 3 cut(s) | 131, 149, 205 |
| DdeI | CTNAG | 1 cut(s) | 238 |
| DriI | GACNNNNNGTC | 2 cut(s) | 121, 188 |
| Eam1104I | CTCTTC | 2 cut(s) | 36, 195 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 121, 188 |
| EarI | CTCTTC | 2 cut(s) | 36, 195 |
| FaeI | CATG | 2 cut(s) | 169, 196 |
| FaiI | YATR | 5 cut(s) | 28, 138, 154, 167, 194 |
| FatI | CATG | 2 cut(s) | 165, 192 |
| FokI | GGATG | 1 cut(s) | 26 |
| FspBI | CTAG | 2 cut(s) | 18, 38 |
| Hin1II | CATG | 2 cut(s) | 169, 196 |
| HinfI | GANTC | 2 cut(s) | 65, 108 |
| Hpy166II | GTNNAC | 1 cut(s) | 182 |
| Hpy8I | GTNNAC | 1 cut(s) | 182 |
| HpyAV | CCTTC | 1 cut(s) | 238 |
| HpyCH4V | TGCA | 1 cut(s) | 35 |
| HpyF3I | CTNAG | 1 cut(s) | 238 |
| Hsp92II | CATG | 2 cut(s) | 169, 196 |
| LguI | GCTCTTC | 2 cut(s) | 36, 195 |
| LpnPI | CCDG | 1 cut(s) | 201 |
| LweI | GCATC | 1 cut(s) | 4 |
| MaeI | CTAG | 2 cut(s) | 18, 38 |
| MboII | GAAGA | 2 cut(s) | 53, 212 |
| MluCI | AATT | 5 cut(s) | 23, 47, 139, 207, 243 |
| MlyI | GAGTC | 1 cut(s) | 59 |
| MnlI | CCTC | 4 cut(s) | 80, 83, 167, 190 |
| MseI | TTAA | 1 cut(s) | 83 |
| NlaIII | CATG | 2 cut(s) | 169, 196 |
| PciSI | GCTCTTC | 2 cut(s) | 36, 195 |
| PfeI | GAWTC | 1 cut(s) | 108 |
| PleI | GAGTC | 1 cut(s) | 59 |
| PpsI | GAGTC | 1 cut(s) | 59 |
| SapI | GCTCTTC | 2 cut(s) | 36, 195 |
| SaqAI | TTAA | 1 cut(s) | 83 |
| SchI | GAGTC | 1 cut(s) | 59 |
| SetI | ASST | 5 cut(s) | 133, 151, 190, 207, 230 |
| SfaNI | GCATC | 1 cut(s) | 4 |
| SfcI | CTRYAG | 1 cut(s) | 126 |
| SgeI | CNNG | 9 cut(s) | 22, 30, 50, 73, 89, 133, 178, 200, 205 |
| Sse9I | AATT | 5 cut(s) | 23, 47, 139, 207, 243 |
| SspMI | CTAG | 2 cut(s) | 18, 38 |
| TasI | AATT | 5 cut(s) | 23, 47, 139, 207, 243 |
| TfiI | GAWTC | 1 cut(s) | 108 |
| Tru1I | TTAA | 1 cut(s) | 83 |
| Tru9I | TTAA | 1 cut(s) | 83 |
| XspI | CTAG | 2 cut(s) | 18, 38 |