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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 10 |
| AclWI | GGATC | 1 cut(s) | 335 |
| AcsI | RAATTY | 1 cut(s) | 255 |
| AcyI | GRCGYC | 1 cut(s) | 7 |
| AfaI | GTAC | 1 cut(s) | 38 |
| AgsI | TTSAA | 3 cut(s) | 124, 131, 160 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 227 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 72, 104 |
| AluBI | AGCT | 3 cut(s) | 170, 244, 345 |
| AluI | AGCT | 3 cut(s) | 170, 244, 345 |
| Alw26I | GTCTC | 1 cut(s) | 280 |
| AlwI | GGATC | 1 cut(s) | 335 |
| ApoI | RAATTY | 1 cut(s) | 255 |
| AspLEI | GCGC | 1 cut(s) | 76 |
| BciVI | GTATCC | 1 cut(s) | 97 |
| BcoDI | GTCTC | 1 cut(s) | 280 |
| BfaI | CTAG | 2 cut(s) | 57, 77 |
| BfmI | CTRYAG | 1 cut(s) | 165 |
| BfuI | GTATCC | 1 cut(s) | 97 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 227 |
| BmsI | GCATC | 1 cut(s) | 43 |
| BsaHI | GRCGYC | 1 cut(s) | 7 |
| Bse1I | ACTGG | 1 cut(s) | 325 |
| BseGI | GGATG | 1 cut(s) | 58 |
| BseMII | CTCAG | 1 cut(s) | 291 |
| BseNI | ACTGG | 1 cut(s) | 325 |
| BseRI | GAGGAG | 1 cut(s) | 101 |
| BsmAI | GTCTC | 1 cut(s) | 280 |
| BsmBI | CGTCTC | 1 cut(s) | 280 |
| Bsp143I | GATC | 1 cut(s) | 327 |
| BspCNI | CTCAG | 1 cut(s) | 290 |
| BspPI | GGATC | 1 cut(s) | 335 |
| BspQI | GCTCTTC | 1 cut(s) | 75 |
| BsrI | ACTGG | 1 cut(s) | 325 |
| BssMI | GATC | 1 cut(s) | 327 |
| BssNI | GRCGYC | 1 cut(s) | 7 |
| Bst6I | CTCTTC | 1 cut(s) | 75 |
| BstACI | GRCGYC | 1 cut(s) | 7 |
| BstDEI | CTNAG | 1 cut(s) | 277 |
| BstF5I | GGATG | 1 cut(s) | 58 |
| BstHHI | GCGC | 1 cut(s) | 76 |
| BstKTI | GATC | 1 cut(s) | 330 |
| BstMAI | GTCTC | 1 cut(s) | 280 |
| BstMBI | GATC | 1 cut(s) | 327 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 82, 295 |
| BstSFI | CTRYAG | 1 cut(s) | 165 |
| BsuI | GTATCC | 1 cut(s) | 97 |
| BtsCI | GGATG | 1 cut(s) | 58 |
| CfoI | GCGC | 1 cut(s) | 76 |
| Csp6I | GTAC | 1 cut(s) | 37 |
| CviAII | CATG | 2 cut(s) | 13, 40 |
| CviJI | RGCY | 3 cut(s) | 170, 244, 345 |
| CviKI_1 | RGCY | 3 cut(s) | 170, 244, 345 |
| CviQI | GTAC | 1 cut(s) | 37 |
| DdeI | CTNAG | 1 cut(s) | 277 |
| DpnI | GATC | 1 cut(s) | 329 |
| DpnII | GATC | 1 cut(s) | 327 |
| DriI | GACNNNNNGTC | 1 cut(s) | 227 |
| Eam1104I | CTCTTC | 1 cut(s) | 75 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 227 |
| EarI | CTCTTC | 1 cut(s) | 75 |
| Esp3I | CGTCTC | 1 cut(s) | 280 |
| FaeI | CATG | 2 cut(s) | 16, 43 |
| FaiI | YATR | 5 cut(s) | 14, 41, 67, 177, 303 |
| FatI | CATG | 2 cut(s) | 12, 39 |
| FokI | GGATG | 1 cut(s) | 65 |
| FspBI | CTAG | 2 cut(s) | 57, 77 |
| GlaI | GCGC | 1 cut(s) | 75 |
| HhaI | GCGC | 1 cut(s) | 76 |
| Hin1I | GRCGYC | 1 cut(s) | 7 |
| Hin1II | CATG | 2 cut(s) | 16, 43 |
| Hin6I | GCGC | 1 cut(s) | 74 |
| HinP1I | GCGC | 1 cut(s) | 74 |
| HinfI | GANTC | 1 cut(s) | 147 |
| Hpy166II | GTNNAC | 1 cut(s) | 221 |
| Hpy188I | TCNGA | 1 cut(s) | 184 |
| Hpy8I | GTNNAC | 1 cut(s) | 221 |
| HpyAV | CCTTC | 1 cut(s) | 277 |
| HpyCH4IV | ACGT | 1 cut(s) | 7 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 82, 295 |
| HpyF3I | CTNAG | 1 cut(s) | 277 |
| HpySE526I | ACGT | 1 cut(s) | 7 |
| Hsp92I | GRCGYC | 1 cut(s) | 7 |
| Hsp92II | CATG | 2 cut(s) | 16, 43 |
| HspAI | GCGC | 1 cut(s) | 74 |
| Kzo9I | GATC | 1 cut(s) | 327 |
| LguI | GCTCTTC | 1 cut(s) | 75 |
| LpnPI | CCDG | 1 cut(s) | 306 |
| LweI | GCATC | 1 cut(s) | 43 |
| MaeI | CTAG | 2 cut(s) | 57, 77 |
| MaeII | ACGT | 1 cut(s) | 7 |
| MaeIII | GTNAC | 2 cut(s) | 8, 229 |
| MalI | GATC | 1 cut(s) | 329 |
| MboI | GATC | 1 cut(s) | 327 |
| MboII | GAAGA | 1 cut(s) | 92 |
| MluCI | AATT | 5 cut(s) | 62, 178, 246, 255, 282 |
| MnlI | CCTC | 3 cut(s) | 119, 122, 206 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 82, 295 |
| NdeII | GATC | 1 cut(s) | 327 |
| NlaIII | CATG | 2 cut(s) | 16, 43 |
| NmuCI | GTSAC | 2 cut(s) | 8, 229 |
| PciSI | GCTCTTC | 1 cut(s) | 75 |
| PfeI | GAWTC | 1 cut(s) | 147 |
| RsaI | GTAC | 1 cut(s) | 38 |
| RsaNI | GTAC | 1 cut(s) | 37 |
| SapI | GCTCTTC | 1 cut(s) | 75 |
| Sau3AI | GATC | 1 cut(s) | 327 |
| SetI | ASST | 5 cut(s) | 10, 172, 246, 269, 347 |
| SfaNI | GCATC | 1 cut(s) | 43 |
| SfcI | CTRYAG | 1 cut(s) | 165 |
| Sse9I | AATT | 5 cut(s) | 62, 178, 246, 255, 282 |
| SspMI | CTAG | 2 cut(s) | 57, 77 |
| TaiI | ACGT | 1 cut(s) | 10 |
| TaqI | TCGA | 1 cut(s) | 28 |
| TasI | AATT | 5 cut(s) | 62, 178, 246, 255, 282 |
| TatI | WGTACW | 1 cut(s) | 36 |
| TfiI | GAWTC | 1 cut(s) | 147 |
| TseFI | GTSAC | 2 cut(s) | 8, 229 |
| Tsp45I | GTSAC | 2 cut(s) | 8, 229 |
| TspDTI | ATGAA | 1 cut(s) | 328 |
| TspGWI | ACGGA | 1 cut(s) | 340 |
| XapI | RAATTY | 1 cut(s) | 255 |
| XspI | CTAG | 2 cut(s) | 57, 77 |
| ZraI | GACGTC | 1 cut(s) | 8 |