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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 134 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 171 |
| AgsI | TTSAA | 3 cut(s) | 85, 92, 121 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 121, 188 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 33, 65 |
| AluBI | AGCT | 3 cut(s) | 131, 149, 203 |
| AluI | AGCT | 3 cut(s) | 131, 149, 203 |
| ApoI | RAATTY | 1 cut(s) | 134 |
| AspLEI | GCGC | 1 cut(s) | 37 |
| BfaI | CTAG | 2 cut(s) | 18, 38 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 121, 188 |
| BmsI | GCATC | 1 cut(s) | 4 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 171 |
| Bse3DI | GCAATG | 1 cut(s) | 61 |
| BseGI | GGATG | 1 cut(s) | 19 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 171 |
| BseMI | GCAATG | 1 cut(s) | 61 |
| BseRI | GAGGAG | 1 cut(s) | 62 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 171 |
| BspQI | GCTCTTC | 1 cut(s) | 36 |
| BsrDI | GCAATG | 1 cut(s) | 61 |
| Bst6I | CTCTTC | 1 cut(s) | 36 |
| BstC8I | GCNNGC | 1 cut(s) | 201 |
| BstF5I | GGATG | 1 cut(s) | 19 |
| BstHHI | GCGC | 1 cut(s) | 37 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 43 |
| BtsCI | GGATG | 1 cut(s) | 19 |
| Cac8I | GCNNGC | 1 cut(s) | 201 |
| CfoI | GCGC | 1 cut(s) | 37 |
| CviAII | CATG | 1 cut(s) | 166 |
| CviJI | RGCY | 3 cut(s) | 131, 149, 203 |
| CviKI_1 | RGCY | 3 cut(s) | 131, 149, 203 |
| DriI | GACNNNNNGTC | 2 cut(s) | 121, 188 |
| Eam1104I | CTCTTC | 1 cut(s) | 36 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 121, 188 |
| EarI | CTCTTC | 1 cut(s) | 36 |
| FaeI | CATG | 1 cut(s) | 169 |
| FaiI | YATR | 2 cut(s) | 28, 167 |
| FatI | CATG | 1 cut(s) | 165 |
| FokI | GGATG | 1 cut(s) | 26 |
| FspBI | CTAG | 2 cut(s) | 18, 38 |
| GlaI | GCGC | 1 cut(s) | 36 |
| HhaI | GCGC | 1 cut(s) | 37 |
| Hin1II | CATG | 1 cut(s) | 169 |
| Hin6I | GCGC | 1 cut(s) | 35 |
| HinP1I | GCGC | 1 cut(s) | 35 |
| HinfI | GANTC | 1 cut(s) | 65 |
| Hpy166II | GTNNAC | 1 cut(s) | 182 |
| Hpy188I | TCNGA | 1 cut(s) | 145 |
| Hpy8I | GTNNAC | 1 cut(s) | 182 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 43 |
| Hsp92II | CATG | 1 cut(s) | 169 |
| HspAI | GCGC | 1 cut(s) | 35 |
| LguI | GCTCTTC | 1 cut(s) | 36 |
| LmnI | GCTCC | 1 cut(s) | 96 |
| LweI | GCATC | 1 cut(s) | 4 |
| MaeI | CTAG | 2 cut(s) | 18, 38 |
| MaeIII | GTNAC | 1 cut(s) | 190 |
| MboII | GAAGA | 1 cut(s) | 53 |
| MluCI | AATT | 6 cut(s) | 6, 23, 107, 134, 139, 205 |
| MlyI | GAGTC | 1 cut(s) | 59 |
| MnlI | CCTC | 3 cut(s) | 80, 83, 167 |
| MseI | TTAA | 2 cut(s) | 9, 138 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 43 |
| NlaIII | CATG | 1 cut(s) | 169 |
| NmuCI | GTSAC | 1 cut(s) | 190 |
| PciSI | GCTCTTC | 1 cut(s) | 36 |
| PleI | GAGTC | 1 cut(s) | 59 |
| PpsI | GAGTC | 1 cut(s) | 59 |
| SapI | GCTCTTC | 1 cut(s) | 36 |
| SaqAI | TTAA | 2 cut(s) | 9, 138 |
| SchI | GAGTC | 1 cut(s) | 59 |
| SetI | ASST | 3 cut(s) | 133, 151, 205 |
| SfaNI | GCATC | 1 cut(s) | 4 |
| SgeI | CNNG | 7 cut(s) | 30, 50, 73, 94, 133, 178, 200 |
| Sse9I | AATT | 6 cut(s) | 6, 23, 107, 134, 139, 205 |
| SspMI | CTAG | 2 cut(s) | 18, 38 |
| TasI | AATT | 6 cut(s) | 6, 23, 107, 134, 139, 205 |
| Tru1I | TTAA | 2 cut(s) | 9, 138 |
| Tru9I | TTAA | 2 cut(s) | 9, 138 |
| TseFI | GTSAC | 1 cut(s) | 190 |
| Tsp45I | GTSAC | 1 cut(s) | 190 |
| TspGWI | ACGGA | 1 cut(s) | 115 |
| XapI | RAATTY | 1 cut(s) | 134 |
| XspI | CTAG | 2 cut(s) | 18, 38 |