Rmu_sc0002121.1_g000004

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002121.1
OG0004921
Physical Location & Seq
Reverse (-)
16968 .. 18474
1507 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1101 bp
atgatcagcttattctgcaaaaacttcatattcaaattgcaaaccacctcagattctgtctcttcgttcacactaccgacgtcaacatcactgttgctgcttccgccgccaccctcaccagcgccgatccctttactggattccggtgatgtcgttacatccgatgttgaggctcagacacttctggtgcacaatatggatgatgatcttgagttgttttggcgggcatcgtttgttccgaggattacagagtttccctataaccgtgtgcctaaagttgctttcatgttcttgacaaagggtcctatacctctagccccattgtgggaaatgttcttcaaagaccatcaaggactttacaacatatattttcaccctcatccatcctatgtagaatcgtggcccaaaaattctgttttctatcgaacaagaattccaagcaagcaagttgaatggggaaaaccaaccatgattgacgccgagagacgtctactagcgagtgccctccttgacttctctaatgaacgattcgtgttgttgtccgagacttgcatcccactattcaacttcactacagtatacaactacctcatgaattccaataagagctttttagcttcctatgatgatccaaggaaagttggccgtggcagatacaatccccaaatggatcctacagtaacaatatcagattggcgaaaagggtcacaatggttcgaggtcaaccgcaaacttgcaatagagatcgtatcagataccaagtactaccccatattcaaaaagtattgcagtcctccttgctacatggatgagcattacatccctaccctagtgaacattatcggccccggtcacaactcaaacaggaccgttacttgggttgattggtccaagagtggtccgcaccctggaagatttgggaggaatggtgtttcagatgaattgctgaatcgaataaggtttggtactaatggtacctgtgatggtgatgttaatacaaccagttctatgtgctttttgtttgctaggaagttcatgccggacactctgcaacctctcttgcaaattgcacccgtgttgctaggttccatttctcaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

366

Amino Acids

41.86

Weight (kDa)

8.13

Isoelectric Point (pI)

32.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 93 - 320 4.6e-80 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 83, 490
Acc65I GGTACC 1 cut(s) 974
AccB1I GGYRCC 1 cut(s) 974
AccI GTMKAC 2 cut(s) 490, 579
AciI CCGC 5 cut(s) 104, 107, 223, 727, 902
AclWI GGATC 4 cut(s) 121, 623, 665, 678
AcoI YGGCCR 1 cut(s) 643
AcsI RAATTY 3 cut(s) 409, 432, 595
AcyI GRCGYC 3 cut(s) 80, 477, 487
AfaI GTAC 3 cut(s) 764, 967, 976
AfiI CCNNNNNNNGG 5 cut(s) 136, 324, 325, 876, 908
AgsI TTSAA 5 cut(s) 34, 340, 452, 565, 778
AhdI GACNNNNNGTC 1 cut(s) 300
AjnI CCWGG 1 cut(s) 907
AloI GAACNNNNNNTCC 2 cut(s) 418, 450
AluBI AGCT 3 cut(s) 9, 609, 617
AluI AGCT 3 cut(s) 9, 609, 617
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 3 cut(s) 64, 478, 539
Alw44I GTGCAC 1 cut(s) 188
AlwI GGATC 4 cut(s) 121, 623, 665, 678
AlwNI CAGNNNCTG 1 cut(s) 56
AoxI GGCC 3 cut(s) 401, 643, 844
ApaLI GTGCAC 1 cut(s) 188
ApeKI GCWGC 1 cut(s) 97
ApoI RAATTY 3 cut(s) 409, 432, 595
Asp718I GGTACC 1 cut(s) 974
AspLEI GCGC 1 cut(s) 124
AspS9I GGNCC 6 cut(s) 302, 402, 845, 867, 888, 899
AsuC2I CCSGG 1 cut(s) 849
AsuHPI GGTGA 4 cut(s) 108, 158, 365, 998
AvaII GGWCC 4 cut(s) 302, 867, 888, 899
BaeGI GKGCMC 2 cut(s) 192, 505
BamHI GGATCC 1 cut(s) 670
BanI GGYRCC 1 cut(s) 974
Bbv12I GWGCWC 1 cut(s) 192
BbvI GCAGC 1 cut(s) 84
BccI CCATC 3 cut(s) 354, 391, 977
BceAI ACGGC 1 cut(s) 630
BciT130I CCWGG 1 cut(s) 909
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 849
BcoDI GTCTC 3 cut(s) 64, 478, 539
BfaI CTAG 5 cut(s) 314, 494, 830, 1026, 1082
BfmI CTRYAG 2 cut(s) 573, 675
BfoI RGCGCY 1 cut(s) 125
BisI GCNGC 2 cut(s) 98, 107
BlsI GCNGC 2 cut(s) 99, 108
BmcAI AGTACT 1 cut(s) 764
Bme1390I CCNGG 2 cut(s) 849, 909
Bme18I GGWCC 4 cut(s) 302, 867, 888, 899
BmeRI GACNNNNNGTC 1 cut(s) 300
BmgT120I GGNCC 6 cut(s) 302, 402, 845, 867, 888, 899
BmiI GGNNCC 5 cut(s) 303, 672, 847, 976, 1087
BmrFI CCNGG 2 cut(s) 849, 909
BmsI GCATC 2 cut(s) 236, 561
BpuEI CTTGAG 1 cut(s) 230
BpuMI CCSGG 1 cut(s) 849
BsaBI GATNNNNATC 1 cut(s) 204
BsaHI GRCGYC 3 cut(s) 80, 477, 487
BsaJI CCNNGG 5 cut(s) 239, 632, 646, 847, 907
BsaWI WCCGGW 1 cut(s) 143
BsaXI ACNNNNNCTCC 2 cut(s) 913, 943
Bsc4I CCNNNNNNNGG 5 cut(s) 136, 324, 325, 876, 908
Bse1I ACTGG 2 cut(s) 141, 1002
Bse8I GATNNNNATC 1 cut(s) 204
BseBI CCWGG 1 cut(s) 909
BseDI CCNNGG 5 cut(s) 239, 632, 646, 847, 907
BseGI GGATG 7 cut(s) 158, 205, 379, 383, 552, 814, 819
BseJI GATNNNNATC 1 cut(s) 204
BseLI CCNNNNNNNGG 5 cut(s) 136, 324, 325, 876, 908
BseMII CTCAG 2 cut(s) 63, 188
BseNI ACTGG 2 cut(s) 141, 1002
BseSI GKGCMC 2 cut(s) 192, 505
BseXI GCAGC 1 cut(s) 84
BshFI GGCC 3 cut(s) 403, 645, 846
BshNI GGYRCC 1 cut(s) 974
BsiHKAI GWGCWC 1 cut(s) 192
BsiSI CCGG 3 cut(s) 144, 849, 1040
BslI CCNNNNNNNGG 5 cut(s) 136, 324, 325, 876, 908
BsmAI GTCTC 3 cut(s) 64, 478, 539
BsmBI CGTCTC 1 cut(s) 478
BsnI GGCC 3 cut(s) 403, 645, 846
Bsp1286I GDGCHC 2 cut(s) 192, 505
Bsp143I GATC 6 cut(s) 3, 126, 205, 628, 670, 744
BspACI CCGC 5 cut(s) 104, 107, 223, 727, 902
BspANI GGCC 3 cut(s) 403, 645, 846
BspCNI CTCAG 2 cut(s) 62, 187
BspHI TCATGA 1 cut(s) 591
BspLI GGNNCC 5 cut(s) 303, 672, 847, 976, 1087
BspPI GGATC 4 cut(s) 121, 623, 665, 678
BspT107I GGYRCC 1 cut(s) 974
BsrI ACTGG 2 cut(s) 141, 1002
BssECI CCNNGG 5 cut(s) 239, 632, 646, 847, 907
BssMI GATC 6 cut(s) 3, 126, 205, 628, 670, 744
BssNAI GTATAC 1 cut(s) 580
BssNI GRCGYC 3 cut(s) 80, 477, 487
BssT1I CCWWGG 1 cut(s) 632
Bst1107I GTATAC 1 cut(s) 580
Bst2UI CCWGG 1 cut(s) 909
Bst4CI ACNGT 5 cut(s) 93, 266, 577, 679, 871
Bst6I CTCTTC 1 cut(s) 67
BstACI GRCGYC 3 cut(s) 80, 477, 487
BstC8I GCNNGC 2 cut(s) 225, 443
BstDEI CTNAG 2 cut(s) 49, 174
BstDSI CCRYGG 1 cut(s) 646
BstF5I GGATG 7 cut(s) 158, 205, 379, 383, 552, 814, 819
BstH2I RGCGCY 1 cut(s) 125
BstHHI GCGC 1 cut(s) 124
BstKTI GATC 6 cut(s) 6, 129, 208, 631, 673, 747
BstMAI GTCTC 3 cut(s) 64, 478, 539
BstMBI GATC 6 cut(s) 3, 126, 205, 628, 670, 744
BstMWI GCNNNNNNNGC 3 cut(s) 15, 103, 106
BstNI CCWGG 1 cut(s) 909
BstSCI CCNGG 2 cut(s) 847, 907
BstSFI CTRYAG 2 cut(s) 573, 675
BstSLI GKGCMC 2 cut(s) 192, 505
BstV1I GCAGC 1 cut(s) 84
BstX2I RGATCY 1 cut(s) 670
BstYI RGATCY 1 cut(s) 670
BstZ17I GTATAC 1 cut(s) 580
BsuRI GGCC 3 cut(s) 403, 645, 846
BtgI CCRYGG 1 cut(s) 646
BtsCI GGATG 7 cut(s) 158, 205, 379, 383, 552, 814, 819
BtsIMutI CAGTG 1 cut(s) 89
Cac8I GCNNGC 2 cut(s) 225, 443
CaiI CAGNNNCTG 1 cut(s) 56
CciI TCATGA 1 cut(s) 591
CfoI GCGC 1 cut(s) 124
Cfr13I GGNCC 6 cut(s) 302, 402, 845, 867, 888, 899
CseI GACGC 1 cut(s) 485
Csp6I GTAC 3 cut(s) 763, 966, 975
CviAII CATG 5 cut(s) 286, 469, 592, 805, 1036
CviJI RGCY 8 cut(s) 9, 173, 317, 403, 609, 617, 645, 846
CviKI_1 RGCY 8 cut(s) 9, 173, 317, 403, 609, 617, 645, 846
CviQI GTAC 3 cut(s) 763, 966, 975
DdeI CTNAG 2 cut(s) 49, 174
DpnI GATC 6 cut(s) 5, 128, 207, 630, 672, 746
DpnII GATC 6 cut(s) 3, 126, 205, 628, 670, 744
DriI GACNNNNNGTC 1 cut(s) 300
EaeI YGGCCR 1 cut(s) 643
Eam1104I CTCTTC 1 cut(s) 67
Eam1105I GACNNNNNGTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 67
EciI GGCGGA 1 cut(s) 93
Eco130I CCWWGG 1 cut(s) 632
Eco47I GGWCC 4 cut(s) 302, 867, 888, 899
EcoO109I RGGNCCY 1 cut(s) 302
EcoRI GAATTC 2 cut(s) 432, 595
EcoRII CCWGG 1 cut(s) 907
EcoT14I CCWWGG 1 cut(s) 632
ErhI CCWWGG 1 cut(s) 632
Esp3I CGTCTC 1 cut(s) 478
FaeI CATG 5 cut(s) 289, 472, 595, 808, 1039
FatI CATG 5 cut(s) 285, 468, 591, 804, 1035
FauI CCCGC 1 cut(s) 216
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 2 cut(s) 490, 579
Fnu4HI GCNGC 2 cut(s) 98, 107
FokI GGATG 7 cut(s) 145, 212, 366, 370, 539, 806, 821
Fsp4HI GCNGC 2 cut(s) 98, 107
FspBI CTAG 5 cut(s) 314, 494, 830, 1026, 1082
GlaI GCGC 1 cut(s) 123
GluI GCNGC 2 cut(s) 98, 107
HaeII RGCGCY 1 cut(s) 125
HaeIII GGCC 3 cut(s) 403, 645, 846
HapII CCGG 3 cut(s) 144, 849, 1040
HgaI GACGC 1 cut(s) 485
HhaI GCGC 1 cut(s) 124
Hin1I GRCGYC 3 cut(s) 80, 477, 487
Hin1II CATG 5 cut(s) 289, 472, 595, 808, 1039
Hin6I GCGC 1 cut(s) 122
HinP1I GCGC 1 cut(s) 122
HincII GTYRAC 2 cut(s) 84, 724
HindII GTYRAC 2 cut(s) 84, 724
HinfI GANTC 5 cut(s) 53, 140, 395, 528, 949
HpaII CCGG 3 cut(s) 144, 849, 1040
HphI GGTGA 4 cut(s) 108, 158, 365, 998
Hpy166II GTNNAC 7 cut(s) 69, 84, 190, 491, 580, 724, 835
Hpy188I TCNGA 8 cut(s) 52, 163, 177, 240, 544, 691, 754, 937
Hpy188III TCNNGA 3 cut(s) 209, 292, 592
Hpy8I GTNNAC 7 cut(s) 69, 84, 190, 491, 580, 724, 835
Hpy99I CGWCG 1 cut(s) 82
HpyCH4III ACNGT 5 cut(s) 93, 266, 577, 679, 871
HpyCH4IV ACGT 2 cut(s) 80, 487
HpyCH4V TGCA 9 cut(s) 18, 40, 190, 552, 737, 789, 1051, 1063, 1070
HpyF10VI GCNNNNNNNGC 3 cut(s) 15, 103, 106
HpyF3I CTNAG 2 cut(s) 49, 174
HpySE526I ACGT 2 cut(s) 80, 487
Hsp92I GRCGYC 3 cut(s) 80, 477, 487
Hsp92II CATG 5 cut(s) 289, 472, 595, 808, 1039
HspAI GCGC 1 cut(s) 122
KpnI GGTACC 1 cut(s) 978
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 6 cut(s) 3, 126, 205, 628, 670, 744
Lsp1109I GCAGC 1 cut(s) 84
LweI GCATC 2 cut(s) 236, 561
MaeI CTAG 5 cut(s) 314, 494, 830, 1026, 1082
MaeII ACGT 2 cut(s) 80, 487
MaeIII GTNAC 5 cut(s) 154, 679, 705, 851, 871
MalI GATC 6 cut(s) 5, 128, 207, 630, 672, 746
MboI GATC 6 cut(s) 3, 126, 205, 628, 670, 744
MboII GAAGA 3 cut(s) 54, 328, 924
MflI RGATCY 1 cut(s) 670
MhlI GDGCHC 2 cut(s) 192, 505
MluCI AATT 6 cut(s) 35, 409, 432, 595, 941, 1065
MseI TTAA 1 cut(s) 993
MspI CCGG 3 cut(s) 144, 849, 1040
MspR9I CCNGG 2 cut(s) 849, 909
MvaI CCWGG 1 cut(s) 909
MwoI GCNNNNNNNGC 3 cut(s) 15, 103, 106
NciI CCSGG 1 cut(s) 849
NdeII GATC 6 cut(s) 3, 126, 205, 628, 670, 744
NlaIII CATG 5 cut(s) 289, 472, 595, 808, 1039
NlaIV GGNNCC 5 cut(s) 303, 672, 847, 976, 1087
NmeAIII GCCGAG 1 cut(s) 505
NmuCI GTSAC 2 cut(s) 705, 851
PagI TCATGA 1 cut(s) 591
PcsI WCGNNNNNNNCGW 2 cut(s) 159, 236
PfeI GAWTC 5 cut(s) 53, 140, 395, 528, 949
PkrI GCNGC 2 cut(s) 99, 108
PpuMI RGGWCCY 1 cut(s) 302
Psp5II RGGWCCY 1 cut(s) 302
Psp6I CCWGG 1 cut(s) 907
PspGI CCWGG 1 cut(s) 907
PspN4I GGNNCC 5 cut(s) 303, 672, 847, 976, 1087
PspPI GGNCC 6 cut(s) 302, 402, 845, 867, 888, 899
PspPPI RGGWCCY 1 cut(s) 302
PstNI CAGNNNCTG 1 cut(s) 56
PsuI RGATCY 1 cut(s) 670
RsaI GTAC 3 cut(s) 764, 967, 976
RsaNI GTAC 3 cut(s) 763, 966, 975
SaqAI TTAA 1 cut(s) 993
SatI GCNGC 2 cut(s) 98, 107
Sau3AI GATC 6 cut(s) 3, 126, 205, 628, 670, 744
Sau96I GGNCC 6 cut(s) 302, 402, 845, 867, 888, 899
ScaI AGTACT 1 cut(s) 764
ScrFI CCNGG 2 cut(s) 849, 909
SduI GDGCHC 2 cut(s) 192, 505
SfaNI GCATC 2 cut(s) 236, 561
SfcI CTRYAG 2 cut(s) 573, 675
SinI GGWCC 4 cut(s) 302, 867, 888, 899
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 6 cut(s) 35, 409, 432, 595, 941, 1065
SsiI CCGC 5 cut(s) 104, 107, 223, 727, 902
SspMI CTAG 5 cut(s) 314, 494, 830, 1026, 1082
StyD4I CCNGG 2 cut(s) 847, 907
StyI CCWWGG 1 cut(s) 632
TaaI ACNGT 5 cut(s) 93, 266, 577, 679, 871
TaiI ACGT 2 cut(s) 83, 490
TaqI TCGA 3 cut(s) 424, 717, 952
TasI AATT 6 cut(s) 35, 409, 432, 595, 941, 1065
TatI WGTACW 1 cut(s) 762
TauI GCSGC 1 cut(s) 109
TfiI GAWTC 5 cut(s) 53, 140, 395, 528, 949
Tru1I TTAA 1 cut(s) 993
Tru9I TTAA 1 cut(s) 993
TscAI CASTG 1 cut(s) 96
TseFI GTSAC 2 cut(s) 705, 851
TseI GCWGC 1 cut(s) 97
Tsp45I GTSAC 2 cut(s) 705, 851
TspDTI ATGAA 6 cut(s) 16, 274, 537, 608, 954, 1024
TspRI CASTG 1 cut(s) 96
VneI GTGCAC 1 cut(s) 188
VpaK11BI GGWCC 4 cut(s) 302, 867, 888, 899
XapI RAATTY 3 cut(s) 409, 432, 595
XmiI GTMKAC 2 cut(s) 490, 579
XspI CTAG 5 cut(s) 314, 494, 830, 1026, 1082
ZraI GACGTC 2 cut(s) 81, 488
ZrmI AGTACT 1 cut(s) 764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.