Rmu_sc0002146.1_g000008

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002146.1
OG0007487
Physical Location & Seq
Forward (+)
54563 .. 55701
1139 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1050 bp
atgcaactaactagtgctttgtgggcaagcaagaactttttactgcaacgtgtagggtatcagacagcaaatcaggaggactgcatgaatgatgcaaagttgctgtccttcgatgatgccagcctcgaaaccaggcaaaaaactgctgacgcgatcttggatttcaagagtaagatgattgatatgaaggacgatagctataagcttgtggtgatggaatacttgagcagcagcctggagctgctggacttctttactgaactggaaaagtttctagactcggctcgtcggagccattcacatatcgaacttgctattgaacagtgtagccgcttggggggtaaacatggagtaggaagggaggaggccaagatgatttccaatatattgagtaacattatggcgtctaatgtcaatgaccactctggtgaaaagttggtcgaaaaggctcattgtttgcttgagaagcagaaagaaatgttttccaaatgtcgcattgaacaagagaagcttgggaaggataagaagagtaatgtttggaggaagctcttgagtcgtatattcatggttgccaagtggctcgttctcgttgttctaggggtgaaattggcctataccgcctataatataacatctggtggagatggtttgtctaatgtggttggagatttgaaaatcaaagcagcatgtttccttgatgtctctgttataatagaaatagcagataactggaatagctgccaccttgaagtatgtcaggttgattttgggctgaagaatgaagcaatgaacataatacaggaaggagctttacattccataagagaaatgggggaaataaaagatctcatcggtaaggtactaaattccattgaatctctccttgctttaagtacaaactttgcaaatggtggaaatgtagaagtggcaatcaagaatattaagctttacctgaagaagtttttggataaaattgaggctttggaggagaaaacaggggaatgcagctctagaattttgaaagcaaggcaagctgttctcatgtattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

349

Amino Acids

39.2

Weight (kDa)

6.42

Isoelectric Point (pI)

41.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 28 - 347 1.5e-21 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 710
AccII CGCG 1 cut(s) 152
AciI CCGC 2 cut(s) 331, 618
AcsI RAATTY 2 cut(s) 865, 1014
AcuI CTGAAG 2 cut(s) 794, 974
AcyI GRCGYC 1 cut(s) 404
AfaI GTAC 2 cut(s) 861, 895
AfiI CCNNNNNNNGG 1 cut(s) 337
AflIII ACRYGT 1 cut(s) 49
AgsI TTSAA 7 cut(s) 166, 320, 500, 673, 749, 875, 1021
AhlI ACTAGT 1 cut(s) 11
AjnI CCWGG 2 cut(s) 131, 234
AjuI GAANNNNNNNTTGG 2 cut(s) 947, 979
AleI CACNNNNGTG 1 cut(s) 426
Alw26I GTCTC 1 cut(s) 706
AoxI GGCC 2 cut(s) 366, 609
ApeKI GCWGC 6 cut(s) 228, 231, 241, 683, 738, 1005
ApoI RAATTY 2 cut(s) 865, 1014
ArsI GACNNNNNNTTYG 2 cut(s) 89, 121
Asp700I GAANNNNTTC 1 cut(s) 270
AsuHPI GGTGA 3 cut(s) 223, 440, 613
BarI GAAGNNNNNNTAC 2 cut(s) 795, 827
BbvI GCAGC 6 cut(s) 228, 240, 243, 695, 725, 1017
BccI CCATC 2 cut(s) 208, 638
BciT130I CCWGG 2 cut(s) 133, 236
BcoDI GTCTC 1 cut(s) 706
BcuI ACTAGT 1 cut(s) 11
BfaI CTAG 4 cut(s) 12, 275, 596, 1011
BglII AGATCT 1 cut(s) 844
BisI GCNGC 7 cut(s) 229, 232, 242, 331, 684, 739, 1006
BlsI GCNGC 7 cut(s) 230, 233, 243, 332, 685, 740, 1007
Bme1390I CCNGG 2 cut(s) 133, 236
BmiI GGNNCC 1 cut(s) 293
BmrFI CCNGG 2 cut(s) 133, 236
BmsI GCATC 2 cut(s) 82, 106
BpmI CTGGAG 1 cut(s) 257
BpuEI CTTGAG 3 cut(s) 244, 482, 571
BsaHI GRCGYC 1 cut(s) 404
Bsc4I CCNNNNNNNGG 1 cut(s) 337
Bse1I ACTGG 2 cut(s) 267, 734
Bse3DI GCAATG 1 cut(s) 792
BseBI CCWGG 2 cut(s) 133, 236
BseLI CCNNNNNNNGG 1 cut(s) 337
BseMI GCAATG 1 cut(s) 792
BseNI ACTGG 2 cut(s) 267, 734
BseRI GAGGAG 2 cut(s) 377, 1001
BseXI GCAGC 6 cut(s) 228, 240, 243, 695, 725, 1017
Bsh1236I CGCG 1 cut(s) 152
BshFI GGCC 2 cut(s) 368, 611
BslI CCNNNNNNNGG 1 cut(s) 337
BsmAI GTCTC 1 cut(s) 706
BsmI GAATGC 1 cut(s) 1007
BsnI GGCC 2 cut(s) 368, 611
Bsp143I GATC 2 cut(s) 153, 844
BspACI CCGC 2 cut(s) 331, 618
BspANI GGCC 2 cut(s) 368, 611
BspFNI CGCG 1 cut(s) 152
BspLI GGNNCC 1 cut(s) 293
BsrDI GCAATG 1 cut(s) 792
BsrI ACTGG 2 cut(s) 267, 734
BssMI GATC 2 cut(s) 153, 844
BssNI GRCGYC 1 cut(s) 404
Bst2UI CCWGG 2 cut(s) 133, 236
Bst4CI ACNGT 1 cut(s) 324
Bst6I CTCTTC 1 cut(s) 521
BstACI GRCGYC 1 cut(s) 404
BstC8I GCNNGC 3 cut(s) 28, 121, 1032
BstFNI CGCG 1 cut(s) 152
BstKTI GATC 2 cut(s) 156, 847
BstMAI GTCTC 1 cut(s) 706
BstMBI GATC 2 cut(s) 153, 844
BstMWI GCNNNNNNNGC 4 cut(s) 23, 466, 617, 1031
BstNI CCWGG 2 cut(s) 133, 236
BstNSI RCATGY 1 cut(s) 690
BstSCI CCNGG 2 cut(s) 131, 234
BstUI CGCG 1 cut(s) 152
BstV1I GCAGC 6 cut(s) 228, 240, 243, 695, 725, 1017
BstX2I RGATCY 1 cut(s) 844
BstYI RGATCY 1 cut(s) 844
BsuRI GGCC 2 cut(s) 368, 611
BtsIMutI CAGTG 1 cut(s) 329
Cac8I GCNNGC 3 cut(s) 28, 121, 1032
CseI GACGC 2 cut(s) 158, 393
Csp6I GTAC 2 cut(s) 860, 894
CviAII CATG 5 cut(s) 85, 347, 565, 687, 1042
CviQI GTAC 2 cut(s) 860, 894
DpnI GATC 2 cut(s) 155, 846
DpnII GATC 2 cut(s) 153, 844
Eam1104I CTCTTC 1 cut(s) 521
EarI CTCTTC 1 cut(s) 521
Eco57I CTGAAG 2 cut(s) 794, 974
EcoRII CCWGG 2 cut(s) 131, 234
FaeI CATG 5 cut(s) 88, 350, 568, 690, 1045
FalI AAGNNNNNCTT 2 cut(s) 495, 527
FatI CATG 5 cut(s) 84, 346, 564, 686, 1041
Fnu4HI GCNGC 7 cut(s) 229, 232, 242, 331, 684, 739, 1006
Fsp4HI GCNGC 7 cut(s) 229, 232, 242, 331, 684, 739, 1006
FspBI CTAG 4 cut(s) 12, 275, 596, 1011
GluI GCNGC 7 cut(s) 229, 232, 242, 331, 684, 739, 1006
GsuI CTGGAG 1 cut(s) 257
HaeIII GGCC 2 cut(s) 368, 611
HgaI GACGC 2 cut(s) 158, 393
Hin1I GRCGYC 1 cut(s) 404
Hin1II CATG 5 cut(s) 88, 350, 568, 690, 1045
HindIII AAGCTT 3 cut(s) 203, 509, 944
HinfI GANTC 3 cut(s) 278, 553, 875
HphI GGTGA 3 cut(s) 223, 440, 613
Hpy166II GTNNAC 1 cut(s) 344
Hpy188I TCNGA 2 cut(s) 63, 291
Hpy188III TCNNGA 6 cut(s) 74, 166, 275, 550, 934, 1011
Hpy8I GTNNAC 1 cut(s) 344
Hpy99I CGWCG 1 cut(s) 291
HpyAV CCTTC 5 cut(s) 118, 181, 351, 511, 797
HpyCH4III ACNGT 1 cut(s) 324
HpyCH4IV ACGT 1 cut(s) 49
HpyCH4V TGCA 6 cut(s) 4, 46, 84, 95, 905, 1005
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 466, 617, 1031
HpySE526I ACGT 1 cut(s) 49
Hsp92I GRCGYC 1 cut(s) 404
Hsp92II CATG 5 cut(s) 88, 350, 568, 690, 1045
Kzo9I GATC 2 cut(s) 153, 844
LmnI GCTCC 3 cut(s) 238, 291, 806
Lsp1109I GCAGC 6 cut(s) 228, 240, 243, 695, 725, 1017
LweI GCATC 2 cut(s) 82, 106
MaeI CTAG 4 cut(s) 12, 275, 596, 1011
MaeII ACGT 1 cut(s) 49
MaeIII GTNAC 1 cut(s) 392
MalI GATC 2 cut(s) 155, 846
MboI GATC 2 cut(s) 153, 844
MboII GAAGA 3 cut(s) 538, 787, 967
MflI RGATCY 1 cut(s) 844
MluCI AATT 4 cut(s) 605, 865, 972, 1014
MlyI GAGTC 2 cut(s) 272, 562
MmeI TCCRAC 2 cut(s) 269, 643
MnlI CCTC 7 cut(s) 70, 134, 355, 358, 534, 970, 979
MroXI GAANNNNTTC 1 cut(s) 270
MseI TTAA 3 cut(s) 890, 942, 1048
MslI CAYNNNNRTG 1 cut(s) 426
MspR9I CCNGG 2 cut(s) 133, 236
Mva1269I GAATGC 1 cut(s) 1007
MvaI CCWGG 2 cut(s) 133, 236
MvnI CGCG 1 cut(s) 152
MwoI GCNNNNNNNGC 4 cut(s) 23, 466, 617, 1031
NdeII GATC 2 cut(s) 153, 844
NlaIII CATG 5 cut(s) 88, 350, 568, 690, 1045
NlaIV GGNNCC 1 cut(s) 293
NmeAIII GCCGAG 1 cut(s) 260
NspI RCATGY 1 cut(s) 690
OliI CACNNNNGTG 1 cut(s) 426
PctI GAATGC 1 cut(s) 1007
PdmI GAANNNNTTC 1 cut(s) 270
PfeI GAWTC 1 cut(s) 875
PkrI GCNGC 7 cut(s) 230, 233, 243, 332, 685, 740, 1007
PleI GAGTC 2 cut(s) 272, 561
PpsI GAGTC 2 cut(s) 272, 561
PsiI TTATAA 1 cut(s) 710
Psp6I CCWGG 2 cut(s) 131, 234
PspGI CCWGG 2 cut(s) 131, 234
PspN4I GGNNCC 1 cut(s) 293
PsuI RGATCY 1 cut(s) 844
RsaI GTAC 2 cut(s) 861, 895
RsaNI GTAC 2 cut(s) 860, 894
RseI CAYNNNNRTG 1 cut(s) 426
SaqAI TTAA 3 cut(s) 890, 942, 1048
SatI GCNGC 7 cut(s) 229, 232, 242, 331, 684, 739, 1006
Sau3AI GATC 2 cut(s) 153, 844
SchI GAGTC 2 cut(s) 272, 562
ScrFI CCNGG 2 cut(s) 133, 236
SfaNI GCATC 2 cut(s) 82, 106
SmiMI CAYNNNNRTG 1 cut(s) 426
SmlI CTYRAG 3 cut(s) 223, 461, 550
SmoI CTYRAG 3 cut(s) 223, 461, 550
SpeI ACTAGT 1 cut(s) 11
Sse9I AATT 4 cut(s) 605, 865, 972, 1014
SsiI CCGC 2 cut(s) 331, 618
SspI AATATT 1 cut(s) 940
SspMI CTAG 4 cut(s) 12, 275, 596, 1011
StyD4I CCNGG 2 cut(s) 131, 234
TaaI ACNGT 1 cut(s) 324
TaiI ACGT 1 cut(s) 52
TaqI TCGA 4 cut(s) 111, 126, 306, 441
TasI AATT 4 cut(s) 605, 865, 972, 1014
TatI WGTACW 1 cut(s) 893
TauI GCSGC 1 cut(s) 333
TfiI GAWTC 1 cut(s) 875
Tru1I TTAA 3 cut(s) 890, 942, 1048
Tru9I TTAA 3 cut(s) 890, 942, 1048
TscAI CASTG 1 cut(s) 329
TseI GCWGC 6 cut(s) 228, 231, 241, 683, 738, 1005
TspDTI ATGAA 5 cut(s) 101, 200, 553, 795, 803
TspRI CASTG 1 cut(s) 329
XapI RAATTY 2 cut(s) 865, 1014
XbaI TCTAGA 2 cut(s) 274, 1010
XceI RCATGY 1 cut(s) 690
XcmI CCANNNNNNNNNTGG 1 cut(s) 826
XmnI GAANNNNTTC 1 cut(s) 270
XspI CTAG 4 cut(s) 12, 275, 596, 1011
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.