Rmu_sc0002469.1_g000001

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002469.1
OG0008637
Physical Location & Seq
Reverse (-)
2245 .. 9061
6817 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3672 bp
atggcggcgagaaagaaaaccaaaggcagaggccggccgaggcaagaagagaacggcgctggtctggaggagatgaaggccgagggtattttgggaattgagaacgagggagctaatggggaagtaaaacaagaagctgtggctcctttggtggagggcagaggcaggaagcggcggaggaagaaacaagaagaagacggtggtggtggtgagggtgttttgggaaatcaaaacgaggaagaggttgctgaagatggtgtggagttaaagacgcgttatccacagagggcggcaagggctgtaagggcggtggtggaggaggaggaggagccggagattcggaagaggacgaagaagagaaaagcagtggtggtggagggggaggaggagccggagattcggaagatgatgaagaagagaaaagcagcggcggcggagggagaggaagatgaggcagtgaaggacgtttcaacaatgtgccatcagtgtcagaggaatgataaagggagagtggttcggtgcacgagttgtccgaaaaggggtaagggaatgaggaagaggtattgtatcccttgcatacagaggtggtatcccaacacatcggaggaagccattgctgaggcttgtcctgtgtgccgcggcaactgcaactgcaaagcctgccttcgactggatgtgcctaataggtgtttgaagaatctggacccgaaaatcaacgaggagaccaagctggagcactgtaagtatttggttcatcagctgcttccgtatttgaagaggattagggacgagcaggtgagtgagatggtggtggaagccaagaggcagggattaagtgatgtttcagagctcaaggtggagaaggctaattgcgctgacgatgagcgtatgtactgcaataactgcagcacttcgatttttgattttcttaggagttgtcctgagtgtgagtatgatctgtgcctcatttgctgccgggagattcgagatggcaagtggaagaagagaggtatggaggaggaagagagtcagaagtatgtcaatttggggcgtgagtatttgcatggaaagggagatttgaagagcttgctaaaaaaaaagagagaaaaggagttgaagagcttgctagagaagaaaagagaagaagagttgaaggagaatgttgatgtgggagtgaagctggactcgtcgtcagaatctgctcaagagtccacgagagcaagtgaagatggaaatattcaagatgggcagcagaaaaagagaggcagagaggatgaagtaagtgaggaatttgttaatgcgggggagtgtttggatgaagagggagagctgaagaggaatgatgatgtgggagtgaagcaggacgagtctgaatctgctgaagagtccacatttgagtggagagcaagtgaagatggaagcattatctgccccccaacacacatgaaaggttgtggtaagcataatttagaattgaggtgcaatttttctcagaatcatgtcatagacttggtagagaaagcaggaaaaatagatgaaagttacaatcctatgcatgctagtagtgaaacatgtgcacaaaagtgttcatgtgttaaacctgtgaatgatgctgtcaactcaagtagcagcagctgtaagttaagaaaagcagcttctagagacagttctgatgataactacttgttctgtccaagggtcggagaaatccaagatgaggattttgggcattttcagtggcattggatgaggggtgagcctgtgatcgttagcaatgcacttcaaaatggatcaggtttgagctgggaaccattagtcatgtggcgtgcatttaggcagataaagaatctcaagcatgatagagaattggcagtccataccattgaatgcttggattggagtttcaaagaaactaataacctccatttctttactggatattccgaaggccggcctgatgagaaaggatggcctgagattctcaagctcaaagacttcccgccatctactgagtttgacaatcgtctcccccggcatggtaaggaatttgtctgttgtctgcctttcaaagagtatactcaccccaacaagggaaccctgaaccttgctatccacttgccacctacctcagtaaagccggacatgggtccaaaaacatatattgcctatggttttgcacaggagcttggacggggagattctgtgaccaagcttcactgcgatatgtctgatgcggttaatattctgacacatacagctgaagtggcccttacaactgaacaacttgcaactatagataagttgaaagaaaagcacaggaagcaagacgaaagagagatatttgggaattgtcacacaagtgctggaaatggaagtgggcctcaggaagaatcagctgaagtatgtgaggatggtggtgccttatgggacattttccggatccaggatgttccgcagctggagaaatacatccacaagcacatcaatgaatttaggcatgtgcactgtaatcctttgcagcgtgttactcatgctatacatgatcagactgtttatctcactgtggagcacaagaggaagctcaaggaggaattcggtatcgagccgtggacatttgtccaaaaacttggagatgctgttttcattcctgcaggttgtcctcatcaggtgaggaacttgaagtcttgtatcaaagttgcactggactttgtttcccctgagaatgtggttactcaggacgtctggccggcgatgaaaccgagccaagagcacgggcgtcaacacgcccccacaaagatcttactgacctcgatctcagcaatggtggccgaaacgacgacgtttccaatcgacctcatgaagacgaggctccagctccacggcgagtcccggtctctcggctcgacccggcccaccaacgcgttccgggtggcctcggaaatcgtgcgcctggaaggccctctgggactgtacaagggtttgtcgccggcgattctcaggcaccttttttacacgcctgttcgtatcgtcggctacgagcacctcagaaactctttgaaaaccgacggtgggtccctctccctcggggctaaagccctctacggcggactctccggcgtcattgctcagttggtggcaagcccggcagatcttgtaaaggtgaggatgcaagcagatggtcgttttgtgagccaaggctttcaacctaggtactcggggtgtttcgatgctttaaacaagatcgcccgagcagaaggtgttggaggactttggaaaggggttttccctaatgttcaaagagctttcttagtaaacatgggagaattagcttgttacgatcatgcaaaacgttttgtaatcaataatcagatttctgaggataatatatatgcccacacattagcatcaatcatgtcgggcctttcggcgactgctttgagctgtccagcagacgtggtaaagacgagaatgatgaatcagtcagaaattaaggaagggaagattatgtacagcagttcttatgattgtcttgtgaagacagtcaaagttgaaggtctgagagctctgtggaaagggttctttcctacatgggcacggcttgggccttggcaatttgttttctgggtctcgtacgagaagtttcgacaaattgcagggttttcttccttctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

1223

Amino Acids

138.04

Weight (kDa)

6.94

Isoelectric Point (pI)

51.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 156 - 223 1.3e-06 Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC PF02373 818 - 903 1.2e-12 JmjC domain, hydroxylase
Mito_carr PF00153 929 - 1021 1.4e-17 Mitochondrial carrier protein
Mito_carr PF00153 1026 - 1122 1.5e-21 Mitochondrial carrier protein
Mito_carr PF00153 1126 - 1217 1.6e-21 Mitochondrial carrier protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 784
AatII GACGTC 1 cut(s) 2742
Acc36I ACCTGC 2 cut(s) 784, 2642
AccB1I GGYRCC 2 cut(s) 2417, 3000
AccI GTMKAC 1 cut(s) 2075
AccII CGCG 3 cut(s) 274, 639, 2921
AccIII TCCGGA 1 cut(s) 2436
AclI AACGTT 1 cut(s) 3349
AclWI GGATC 3 cut(s) 1798, 2434, 2447
AcoI YGGCCR 3 cut(s) 35, 2744, 2826
AcsI RAATTY 4 cut(s) 1289, 2045, 2489, 2591
AcuI CTGAAG 5 cut(s) 270, 1352, 1401, 2280, 2418
AcyI GRCGYC 3 cut(s) 2739, 2776, 3117
AfaI GTAC 5 cut(s) 893, 2972, 3212, 3509, 3632
AflIII ACRYGT 3 cut(s) 272, 1571, 2919
AhdI GACNNNNNGTC 2 cut(s) 1189, 3070
AjiI CACGTC 1 cut(s) 3454
AjnI CCWGG 2 cut(s) 2442, 2949
AleI CACNNNNGTG 2 cut(s) 1582, 2358
Alw21I GWGCWC 9 cut(s) 524, 738, 852, 1579, 2505, 2571, 2772, 3042, 3565
Alw26I GTCTC 5 cut(s) 716, 1656, 2030, 2898, 3631
Alw44I GTGCAC 3 cut(s) 520, 1575, 2501
AlwI GGATC 3 cut(s) 1798, 2434, 2447
AlwNI CAGNNNCTG 2 cut(s) 1199, 1635
Ama87I CYCGRG 3 cut(s) 3083, 3214, 3246
Aor13HI TCCGGA 1 cut(s) 2436
ApaLI GTGCAC 3 cut(s) 520, 1575, 2501
ApoI RAATTY 4 cut(s) 1289, 2045, 2489, 2591
Asp700I GAANNNNTTC 1 cut(s) 3575
AspA2I CCTAGG 1 cut(s) 3206
AspLEI GCGC 3 cut(s) 59, 875, 2949
AspS9I GGNCC 9 cut(s) 703, 2147, 2266, 2378, 2910, 2957, 3072, 3418, 3602
AsuC2I CCSGG 6 cut(s) 977, 2032, 2890, 2908, 2927, 3143
AsuHPI GGTGA 6 cut(s) 221, 808, 1766, 2072, 2680, 3172
AvaI CYCGRG 3 cut(s) 3083, 3214, 3246
AvaII GGWCC 3 cut(s) 703, 2147, 3072
AvrII CCTAGG 1 cut(s) 3206
AxyI CCTNAGG 1 cut(s) 2382
BaeGI GKGCMC 4 cut(s) 524, 1579, 2505, 3595
BamHI GGATCC 1 cut(s) 2439
BanI GGYRCC 2 cut(s) 2417, 3000
BanII GRGCYC 2 cut(s) 852, 3565
BarI GAAGNNNNNNTAC 2 cut(s) 3491, 3523
BauI CACGAG 2 cut(s) 523, 1213
BbsI GAAGAC 3 cut(s) 201, 2867, 3542
Bbv12I GWGCWC 9 cut(s) 524, 738, 852, 1579, 2505, 2571, 2772, 3042, 3565
BbvCI CCTCAGC 1 cut(s) 618
BceAI ACGGC 5 cut(s) 70, 2590, 2896, 3118, 3611
BciT130I CCWGG 2 cut(s) 2444, 2951
BciVI GTATCC 2 cut(s) 578, 600
BclI TGATCA 1 cut(s) 2542
BcnI CCSGG 6 cut(s) 977, 2032, 2890, 2908, 2927, 3143
BcoDI GTCTC 5 cut(s) 716, 1656, 2030, 2898, 3631
BfaI CTAG 4 cut(s) 1127, 1560, 1659, 3207
BfmI CTRYAG 3 cut(s) 904, 2292, 2649
BfoI RGCGCY 1 cut(s) 60
BfuAI ACCTGC 2 cut(s) 784, 2642
BfuI GTATCC 2 cut(s) 578, 600
BglI GCCNNNNNGGC 1 cut(s) 2955
BglII AGATCT 2 cut(s) 2796, 3148
BlnI CCTAGG 1 cut(s) 3206
Bme1390I CCNGG 8 cut(s) 977, 2032, 2444, 2890, 2908, 2927, 2951, 3143
Bme18I GGWCC 3 cut(s) 703, 2147, 3072
BmeRI GACNNNNNGTC 2 cut(s) 1189, 3070
BmeT110I CYCGRG 3 cut(s) 3083, 3214, 3246
BmgBI CACGTC 1 cut(s) 3454
BmgT120I GGNCC 9 cut(s) 703, 2147, 2266, 2378, 2910, 2957, 3072, 3418, 3602
BmrFI CCNGG 8 cut(s) 977, 2032, 2444, 2890, 2908, 2927, 2951, 3143
BmsI GCATC 6 cut(s) 1600, 2221, 2623, 3156, 3217, 3413
BoxI GACNNNNGTC 3 cut(s) 2022, 2145, 2615
BpiI GAAGAC 3 cut(s) 201, 2867, 3542
BpmI CTGGAG 4 cut(s) 86, 752, 2480, 2855
Bpu10I CCTNAGC 1 cut(s) 618
BpuEI CTTGAG 6 cut(s) 836, 1188, 1606, 1835, 1967, 2567
BpuMI CCSGG 6 cut(s) 977, 2032, 2890, 2908, 2927, 3143
BsaHI GRCGYC 3 cut(s) 2739, 2776, 3117
BsaI GGTCTC 3 cut(s) 716, 2898, 3631
BsaWI WCCGGW 1 cut(s) 2436
BsaXI ACNNNNNCTCC 6 cut(s) 1393, 1423, 2187, 2217, 2622, 2652
Bse118I RCCGGY 4 cut(s) 33, 1950, 2746, 2986
Bse1I ACTGG 3 cut(s) 675, 1939, 2706
Bse21I CCTNAGG 1 cut(s) 2382
Bse3DI GCAATG 4 cut(s) 612, 1780, 2826, 3120
BseAI TCCGGA 1 cut(s) 2436
BseBI CCWGG 2 cut(s) 2444, 2951
BseGI GGATG 9 cut(s) 679, 1279, 1321, 1752, 1973, 2416, 2452, 2469, 3171
BseMI GCAATG 4 cut(s) 612, 1780, 2826, 3120
BseNI ACTGG 3 cut(s) 675, 1939, 2706
BseRI GAGGAG 9 cut(s) 83, 332, 335, 338, 341, 398, 401, 734, 1031
BseSI GKGCMC 4 cut(s) 524, 1579, 2505, 3595
BseX3I CGGCCG 1 cut(s) 35
BseYI CCCAGC 1 cut(s) 1803
Bsh1236I CGCG 3 cut(s) 274, 639, 2921
Bsh1285I CGRYCG 1 cut(s) 38
BshNI GGYRCC 2 cut(s) 2417, 3000
BsiEI CGRYCG 1 cut(s) 38
BsiHKAI GWGCWC 9 cut(s) 524, 738, 852, 1579, 2505, 2571, 2772, 3042, 3565
BsiHKCI CYCGRG 3 cut(s) 3083, 3214, 3246
BsiWI CGTACG 1 cut(s) 3630
BslFI GGGAC 5 cut(s) 800, 2441, 2872, 2979, 3058
BsmAI GTCTC 5 cut(s) 716, 1656, 2030, 2898, 3631
BsmBI CGTCTC 1 cut(s) 2030
BsmFI GGGAC 5 cut(s) 800, 2441, 2872, 2979, 3058
BsmI GAATGC 1 cut(s) 1892
Bso31I GGTCTC 3 cut(s) 716, 2898, 3631
BsoBI CYCGRG 3 cut(s) 3083, 3214, 3246
Bsp13I TCCGGA 1 cut(s) 2436
Bsp1407I TGTACA 2 cut(s) 2970, 3507
BspEI TCCGGA 1 cut(s) 2436
BspFNI CGCG 3 cut(s) 274, 639, 2921
BspHI TCATGA 1 cut(s) 2856
BspMAI CTGCAG 2 cut(s) 908, 2653
BspMI ACCTGC 2 cut(s) 784, 2642
BspPI GGATC 3 cut(s) 1798, 2434, 2447
BspQI GCTCTTC 2 cut(s) 1076, 1112
BspT107I GGYRCC 2 cut(s) 2417, 3000
BspTNI GGTCTC 3 cut(s) 716, 2898, 3631
BsrDI GCAATG 4 cut(s) 612, 1780, 2826, 3120
BsrFI RCCGGY 4 cut(s) 33, 1950, 2746, 2986
BsrGI TGTACA 2 cut(s) 2970, 3507
BsrI ACTGG 3 cut(s) 675, 1939, 2706
BssAI RCCGGY 4 cut(s) 33, 1950, 2746, 2986
BssNAI GTATAC 1 cut(s) 2076
BssNI GRCGYC 3 cut(s) 2739, 2776, 3117
BssSI CACGAG 2 cut(s) 523, 1213
BssT1I CCWWGG 4 cut(s) 1694, 3193, 3206, 3605
Bst1107I GTATAC 1 cut(s) 2076
Bst2BI CACGAG 2 cut(s) 523, 1213
Bst2UI CCWGG 2 cut(s) 2444, 2951
Bst4CI ACNGT 9 cut(s) 200, 740, 1667, 2507, 2551, 2563, 2970, 3068, 3541
BstACI GRCGYC 3 cut(s) 2739, 2776, 3117
BstAPI GCANNNNNTGC 3 cut(s) 660, 903, 1428
BstAUI TGTACA 2 cut(s) 2970, 3507
BstDSI CCRYGG 3 cut(s) 637, 2606, 2878
BstF5I GGATG 9 cut(s) 679, 1279, 1321, 1752, 1973, 2416, 2452, 2469, 3171
BstFNI CGCG 3 cut(s) 274, 639, 2921
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 3 cut(s) 59, 875, 2949
BstMAI GTCTC 5 cut(s) 716, 1656, 2030, 2898, 3631
BstMCI CGRYCG 1 cut(s) 38
BstNI CCWGG 2 cut(s) 2444, 2951
BstNSI RCATGY 3 cut(s) 1559, 1575, 2501
BstPAI GACNNNNGTC 3 cut(s) 2022, 2145, 2615
BstSCI CCNGG 8 cut(s) 975, 2030, 2442, 2888, 2906, 2925, 2949, 3141
BstSFI CTRYAG 3 cut(s) 904, 2292, 2649
BstSLI GKGCMC 4 cut(s) 524, 1579, 2505, 3595
BstUI CGCG 3 cut(s) 274, 639, 2921
BstV2I GAAGAC 3 cut(s) 201, 2867, 3542
BstX2I RGATCY 3 cut(s) 2439, 2796, 3148
BstXI CCANNNNNNTGG 1 cut(s) 2627
BstYI RGATCY 3 cut(s) 2439, 2796, 3148
BstZ17I GTATAC 1 cut(s) 2076
BstZI CGGCCG 1 cut(s) 35
Bsu36I CCTNAGG 1 cut(s) 2382
BsuI GTATCC 2 cut(s) 578, 600
BtgI CCRYGG 3 cut(s) 637, 2606, 2878
BtgZI GCGATG 1 cut(s) 2765
BtrI CACGTC 1 cut(s) 3454
BtsCI GGATG 9 cut(s) 679, 1279, 1321, 1752, 1973, 2416, 2452, 2469, 3171
BtsI GCAGTG 3 cut(s) 372, 462, 2215
BtsIMutI CAGTG 9 cut(s) 372, 462, 491, 736, 1742, 2215, 2503, 2559, 2699
BveI ACCTGC 2 cut(s) 784, 2642
CaiI CAGNNNCTG 2 cut(s) 1199, 1635
CciI TCATGA 1 cut(s) 2856
CfoI GCGC 3 cut(s) 59, 875, 2949
Cfr10I RCCGGY 4 cut(s) 33, 1950, 2746, 2986
Cfr13I GGNCC 9 cut(s) 703, 2147, 2266, 2378, 2910, 2957, 3072, 3418, 3602
Cfr42I CCGCGG 1 cut(s) 640
CseI GACGC 3 cut(s) 280, 2765, 3106
Csp6I GTAC 5 cut(s) 892, 2971, 3211, 3508, 3631
CviQI GTAC 5 cut(s) 892, 2971, 3211, 3508, 3631
DraI TTTAAA 1 cut(s) 3234
DriI GACNNNNNGTC 2 cut(s) 1189, 3070
EaeI YGGCCR 3 cut(s) 35, 2744, 2826
EagI CGGCCG 1 cut(s) 35
Eam1105I GACNNNNNGTC 2 cut(s) 1189, 3070
EciI GGCGGA 3 cut(s) 190, 449, 3120
Ecl136II GAGCTC 2 cut(s) 850, 3563
EclXI CGGCCG 1 cut(s) 35
Eco130I CCWWGG 4 cut(s) 1694, 3193, 3206, 3605
Eco24I GRGCYC 2 cut(s) 852, 3565
Eco31I GGTCTC 3 cut(s) 716, 2898, 3631
Eco47I GGWCC 3 cut(s) 703, 2147, 3072
Eco52I CGGCCG 1 cut(s) 35
Eco53kI GAGCTC 2 cut(s) 850, 3563
Eco57I CTGAAG 5 cut(s) 270, 1352, 1401, 2280, 2418
Eco81I CCTNAGG 1 cut(s) 2382
Eco88I CYCGRG 3 cut(s) 3083, 3214, 3246
EcoICRI GAGCTC 2 cut(s) 850, 3563
EcoO109I RGGNCCY 2 cut(s) 2957, 3072
EcoRI GAATTC 1 cut(s) 2591
EcoRII CCWGG 2 cut(s) 2442, 2949
EcoT14I CCWWGG 4 cut(s) 1694, 3193, 3206, 3605
EcoT22I ATGCAT 1 cut(s) 1557
EcoT38I GRGCYC 2 cut(s) 852, 3565
ErhI CCWWGG 4 cut(s) 1694, 3193, 3206, 3605
Esp3I CGTCTC 1 cut(s) 2030
FalI AAGNNNNNCTT 4 cut(s) 648, 680, 2253, 2285
FaqI GGGAC 5 cut(s) 800, 2441, 2872, 2979, 3058
FauI CCCGC 2 cut(s) 1294, 2007
FbaI TGATCA 1 cut(s) 2542
FblI GTMKAC 1 cut(s) 2075
FokI GGATG 9 cut(s) 686, 1286, 1328, 1759, 1980, 2423, 2456, 2459, 3178
FriOI GRGCYC 2 cut(s) 852, 3565
FseI GGCCGGCC 2 cut(s) 37, 1954
FspBI CTAG 4 cut(s) 1127, 1560, 1659, 3207
GlaI GCGC 3 cut(s) 58, 874, 2948
GsaI CCCAGC 1 cut(s) 1807
GsuI CTGGAG 4 cut(s) 86, 752, 2480, 2855
HaeII RGCGCY 1 cut(s) 60
HgaI GACGC 3 cut(s) 280, 2765, 3106
HhaI GCGC 3 cut(s) 59, 875, 2949
Hin1I GRCGYC 3 cut(s) 2739, 2776, 3117
Hin6I GCGC 3 cut(s) 57, 873, 2947
HinP1I GCGC 3 cut(s) 57, 873, 2947
HincII GTYRAC 2 cut(s) 1618, 2780
HindII GTYRAC 2 cut(s) 1618, 2780
HindIII AAGCTT 1 cut(s) 2210
HphI GGTGA 6 cut(s) 221, 808, 1766, 2072, 2680, 3172
Hpy99I CGWCG 5 cut(s) 1192, 2839, 2842, 3032, 3068
HpyCH4III ACNGT 9 cut(s) 200, 740, 1667, 2507, 2551, 2563, 2970, 3068, 3541
HpyCH4IV ACGT 5 cut(s) 465, 2739, 2840, 3349, 3453
HpySE526I ACGT 5 cut(s) 465, 2739, 2840, 3349, 3453
Hsp92I GRCGYC 3 cut(s) 2739, 2776, 3117
HspAI GCGC 3 cut(s) 57, 873, 2947
KflI GGGWCCC 1 cut(s) 3072
Kpn2I TCCGGA 1 cut(s) 2436
KroI GCCGGC 4 cut(s) 33, 1950, 2746, 2986
KroNI GCCGGC 4 cut(s) 35, 1952, 2748, 2988
Ksp22I TGATCA 1 cut(s) 2542
KspI CCGCGG 1 cut(s) 640
LguI GCTCTTC 2 cut(s) 1076, 1112
LmnI GCTCC 9 cut(s) 110, 148, 328, 388, 733, 2182, 2566, 2874, 2880
LweI GCATC 6 cut(s) 1600, 2221, 2623, 3156, 3217, 3413
MaeI CTAG 4 cut(s) 1127, 1560, 1659, 3207
MaeII ACGT 5 cut(s) 465, 2739, 2840, 3349, 3453
MaeIII GTNAC 6 cut(s) 1541, 2203, 2351, 2524, 2728, 3332
MflI RGATCY 3 cut(s) 2439, 2796, 3148
MluI ACGCGT 2 cut(s) 272, 2919
MlyI GAGTC 7 cut(s) 1036, 1178, 1217, 1376, 1394, 2894, 3102
MmeI TCCRAC 2 cut(s) 1681, 3241
Mph1103I ATGCAT 1 cut(s) 1557
MreI CGCCGGCG 1 cut(s) 2986
MroI TCCGGA 1 cut(s) 2436
MroNI GCCGGC 4 cut(s) 33, 1950, 2746, 2986
MroXI GAANNNNTTC 1 cut(s) 3575
MseI TTAA 8 cut(s) 266, 833, 1296, 1596, 1643, 2238, 3233, 3489
MslI CAYNNNNRTG 4 cut(s) 1396, 1582, 2358, 2484
MspA1I CMGCKG 7 cut(s) 428, 639, 760, 1635, 2258, 2396, 2458
MspR9I CCNGG 8 cut(s) 977, 2032, 2444, 2890, 2908, 2927, 2951, 3143
Mva1269I GAATGC 1 cut(s) 1892
MvaI CCWGG 2 cut(s) 2444, 2951
MvnI CGCG 3 cut(s) 274, 639, 2921
NaeI GCCGGC 4 cut(s) 35, 1952, 2748, 2988
NciI CCSGG 6 cut(s) 977, 2032, 2890, 2908, 2927, 3143
NgoMIV GCCGGC 4 cut(s) 33, 1950, 2746, 2986
NmeAIII GCCGAG 3 cut(s) 63, 106, 2877
NmuCI GTSAC 2 cut(s) 2203, 2351
NsiI ATGCAT 1 cut(s) 1557
NspI RCATGY 3 cut(s) 1559, 1575, 2501
OliI CACNNNNGTG 2 cut(s) 1582, 2358
PaeI GCATGC 1 cut(s) 1559
PagI TCATGA 1 cut(s) 2856
PaqCI CACCTGC 1 cut(s) 784
PciI ACATGT 1 cut(s) 1571
PciSI GCTCTTC 2 cut(s) 1076, 1112
PcsI WCGNNNNNNNCGW 2 cut(s) 530, 3033
PctI GAATGC 1 cut(s) 1892
PdiI GCCGGC 4 cut(s) 35, 1952, 2748, 2988
PdmI GAANNNNTTC 1 cut(s) 3575
Pfl23II CGTACG 1 cut(s) 3630
PfoI TCCNGGA 1 cut(s) 2442
PleI GAGTC 7 cut(s) 1035, 1178, 1216, 1375, 1393, 2893, 3102
PpsI GAGTC 7 cut(s) 1035, 1178, 1216, 1375, 1393, 2893, 3102
PpuMI RGGWCCY 1 cut(s) 3072
PscI ACATGT 1 cut(s) 1571
PshAI GACNNNNGTC 3 cut(s) 2022, 2145, 2615
Psp124BI GAGCTC 2 cut(s) 852, 3565
Psp1406I AACGTT 1 cut(s) 3349
Psp5II RGGWCCY 1 cut(s) 3072
Psp6I CCWGG 2 cut(s) 2442, 2949
PspFI CCCAGC 1 cut(s) 1803
PspGI CCWGG 2 cut(s) 2442, 2949
PspLI CGTACG 1 cut(s) 3630
PspPI GGNCC 9 cut(s) 703, 2147, 2266, 2378, 2910, 2957, 3072, 3418, 3602
PspPPI RGGWCCY 1 cut(s) 3072
PstI CTGCAG 2 cut(s) 908, 2653
PstNI CAGNNNCTG 2 cut(s) 1199, 1635
PsuI RGATCY 3 cut(s) 2439, 2796, 3148
PvuII CAGCTG 5 cut(s) 760, 1635, 2258, 2396, 2458
RigI GGCCGGCC 2 cut(s) 37, 1954
RsaI GTAC 5 cut(s) 893, 2972, 3212, 3509, 3632
RsaNI GTAC 5 cut(s) 892, 2971, 3211, 3508, 3631
RseI CAYNNNNRTG 4 cut(s) 1396, 1582, 2358, 2484
SacI GAGCTC 2 cut(s) 852, 3565
SacII CCGCGG 1 cut(s) 640
SapI GCTCTTC 2 cut(s) 1076, 1112
SaqAI TTAA 8 cut(s) 266, 833, 1296, 1596, 1643, 2238, 3233, 3489
Sau96I GGNCC 9 cut(s) 703, 2147, 2266, 2378, 2910, 2957, 3072, 3418, 3602
SbfI CCTGCAGG 1 cut(s) 2653
SchI GAGTC 7 cut(s) 1036, 1178, 1217, 1376, 1394, 2894, 3102
ScrFI CCNGG 8 cut(s) 977, 2032, 2444, 2890, 2908, 2927, 2951, 3143
SdaI CCTGCAGG 1 cut(s) 2653
SfaNI GCATC 6 cut(s) 1600, 2221, 2623, 3156, 3217, 3413
SfcI CTRYAG 3 cut(s) 904, 2292, 2649
Sfr303I CCGCGG 1 cut(s) 640
SgrAI CRCCGGYG 1 cut(s) 2986
SgrBI CCGCGG 1 cut(s) 640
SinI GGWCC 3 cut(s) 703, 2147, 3072
SmiMI CAYNNNNRTG 4 cut(s) 1396, 1582, 2358, 2484
SmlI CTYRAG 6 cut(s) 851, 1203, 1621, 1850, 1982, 2582
SmoI CTYRAG 6 cut(s) 851, 1203, 1621, 1850, 1982, 2582
SphI GCATGC 1 cut(s) 1559
Sse8387I CCTGCAGG 1 cut(s) 2653
SspI AATATT 2 cut(s) 1237, 2242
SspMI CTAG 4 cut(s) 1127, 1560, 1659, 3207
SstI GAGCTC 2 cut(s) 852, 3565
StyD4I CCNGG 8 cut(s) 975, 2030, 2442, 2888, 2906, 2925, 2949, 3141
StyI CCWWGG 4 cut(s) 1694, 3193, 3206, 3605
TaaI ACNGT 9 cut(s) 200, 740, 1667, 2507, 2551, 2563, 2970, 3068, 3541
TaiI ACGT 5 cut(s) 468, 2742, 2843, 3352, 3456
TaqI TCGA 9 cut(s) 667, 914, 985, 2601, 2810, 2850, 2903, 3225, 3643
TatI WGTACW 3 cut(s) 891, 2970, 3507
TauI GCSGC 7 cut(s) 8, 175, 293, 431, 434, 639, 642
Tru1I TTAA 8 cut(s) 266, 833, 1296, 1596, 1643, 2238, 3233, 3489
Tru9I TTAA 8 cut(s) 266, 833, 1296, 1596, 1643, 2238, 3233, 3489
TscAI CASTG 9 cut(s) 372, 462, 491, 743, 1742, 2222, 2510, 2566, 2706
TseFI GTSAC 2 cut(s) 2203, 2351
Tsp45I GTSAC 2 cut(s) 2203, 2351
TspGWI ACGGA 1 cut(s) 756
TspRI CASTG 9 cut(s) 372, 462, 491, 743, 1742, 2222, 2510, 2566, 2706
VneI GTGCAC 3 cut(s) 520, 1575, 2501
VpaK11BI GGWCC 3 cut(s) 703, 2147, 3072
XapI RAATTY 4 cut(s) 1289, 2045, 2489, 2591
XbaI TCTAGA 1 cut(s) 1658
XceI RCATGY 3 cut(s) 1559, 1575, 2501
XcmI CCANNNNNNNNNTGG 3 cut(s) 1818, 1888, 1931
XmaJI CCTAGG 1 cut(s) 3206
XmiI GTMKAC 1 cut(s) 2075
XmnI GAANNNNTTC 1 cut(s) 3575
XspI CTAG 4 cut(s) 1127, 1560, 1659, 3207
ZraI GACGTC 1 cut(s) 2740
Zsp2I ATGCAT 1 cut(s) 1557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.