Rroxscaffold_5G00380320

Belongs to the mitochondrial carrier (TC 2.A.29) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0008637
Physical Location & Seq
Reverse (-)
60500927 .. 60524354
23428 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00380320.1

Sequence Viewer

Length: 705 bp
ATGTCGGGCCTTTCAGCGACTGCTTTGAGCTGTCCAGCAGACATGGTAAAGACGAGAAAGGTGAATCAATCAGAAATCAAGGAAGGTAAGATTATGTACAGCAGTTCTTATGATTGTCTTGTGAAGACTGTCAAAGTTGAAGGTCTGAGAGCTCTGTGGAAAGGGTTCTTTCCTACATGGGCACGGCTTGGGCCTTGGCAATTTGTGTTCTGGGTCTCGTATGAGAGGTTTCGACAAATTGCAGGAAAAATCCTTTCAGATCTCACCCATAATTGCCAAAGGCCTAAGCAAGTTCAGTCCAAATTGGTGATCGGTAAGGCATTCAAAAATGCAATTTACAACAATGAAGACAAACGGTTCTGGTTCGACAGATTCGAAACCAAGACTTTGCCTTATCTTTTTCCCCACCTCACGTACCACCCAAATAATAGCTCGACTGTACGTGTGCCTATCGAGCACTTCAAATGCATGTATGTACTTCAAATGCATGTATGTGAGACAGTGTGGAGAAAGATTCTTCTTTGGTCTGATCCTGATCGCGGAGAAGGCTGTATAGTTAAGAGTGTCCGAAACCCCTGTTTTGAACTAAAATCCCCAAAGTGGCGCCAATGGAGGCAAAACCACATCCGAGACCTCCCAAAGTCTCCAGAATACGTTCACGATCGATGTGACCAAACCACAAATTACATCAACAATAACCGTTAG

Protein Analysis

234

Amino Acids

27.73

Weight (kDa)

9.62

Isoelectric Point (pI)

43.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_carr PF00153 1 - 80 1.1e-16 Mitochondrial carrier protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 603
AccII CGCG 1 cut(s) 540
AciI CCGC 1 cut(s) 540
AclWI GGATC 1 cut(s) 524
AcyI GRCGYC 1 cut(s) 604
AfaI GTAC 4 cut(s) 98, 416, 441, 477
AfiI CCNNNNNNNGG 2 cut(s) 539, 600
AflIII ACRYGT 1 cut(s) 442
AgsI TTSAA 5 cut(s) 140, 325, 463, 482, 584
AluBI AGCT 3 cut(s) 30, 152, 432
AluI AGCT 3 cut(s) 30, 152, 432
Alw21I GWGCWC 2 cut(s) 154, 459
Alw26I GTCTC 4 cut(s) 220, 491, 624, 648
AlwI GGATC 1 cut(s) 524
AlwNI CAGNNNCTG 1 cut(s) 20
AoxI GGCC 3 cut(s) 7, 191, 281
Asp700I GAANNNNTTC 2 cut(s) 164, 654
AspLEI GCGC 1 cut(s) 606
AspS9I GGNCC 2 cut(s) 7, 191
AsuHPI GGTGA 3 cut(s) 73, 256, 319
AsuII TTCGAA 1 cut(s) 375
BaeGI GKGCMC 1 cut(s) 184
BanI GGYRCC 1 cut(s) 603
BanII GRGCYC 1 cut(s) 154
BbsI GAAGAC 2 cut(s) 131, 354
Bbv12I GWGCWC 2 cut(s) 154, 459
BceAI ACGGC 1 cut(s) 200
BcoDI GTCTC 4 cut(s) 220, 491, 624, 648
BfoI RGCGCY 1 cut(s) 607
BglII AGATCT 1 cut(s) 259
BmgT120I GGNCC 2 cut(s) 7, 191
BmiI GGNNCC 1 cut(s) 605
BpiI GAAGAC 2 cut(s) 131, 354
BpmI CTGGAG 1 cut(s) 630
Bpu10I CCTNAGC 1 cut(s) 285
Bpu14I TTCGAA 1 cut(s) 375
Bsa29I ATCGAT 1 cut(s) 664
BsaAI YACGTR 2 cut(s) 414, 443
BsaBI GATNNNNATC 1 cut(s) 534
BsaHI GRCGYC 1 cut(s) 604
BsaI GGTCTC 2 cut(s) 220, 624
BsaJI CCNNGG 1 cut(s) 194
Bsc4I CCNNNNNNNGG 2 cut(s) 539, 600
Bse8I GATNNNNATC 1 cut(s) 534
BseCI ATCGAT 1 cut(s) 664
BseDI CCNNGG 1 cut(s) 194
BseGI GGATG 1 cut(s) 624
BseJI GATNNNNATC 1 cut(s) 534
BseLI CCNNNNNNNGG 2 cut(s) 539, 600
BseMII CTCAG 1 cut(s) 137
BseSI GKGCMC 1 cut(s) 184
Bsh1236I CGCG 1 cut(s) 540
Bsh1285I CGRYCG 1 cut(s) 664
BshFI GGCC 3 cut(s) 9, 193, 283
BshNI GGYRCC 1 cut(s) 603
BshVI ATCGAT 1 cut(s) 664
BsiEI CGRYCG 1 cut(s) 664
BsiHKAI GWGCWC 2 cut(s) 154, 459
BslI CCNNNNNNNGG 2 cut(s) 539, 600
BsmAI GTCTC 4 cut(s) 220, 491, 624, 648
BsmI GAATGC 1 cut(s) 320
BsnI GGCC 3 cut(s) 9, 193, 283
Bso31I GGTCTC 2 cut(s) 220, 624
Bsp119I TTCGAA 1 cut(s) 375
Bsp1286I GDGCHC 3 cut(s) 154, 184, 459
Bsp1407I TGTACA 1 cut(s) 96
Bsp143I GATC 5 cut(s) 259, 309, 529, 535, 661
BspACI CCGC 1 cut(s) 540
BspANI GGCC 3 cut(s) 9, 193, 283
BspCNI CTCAG 1 cut(s) 138
BspDI ATCGAT 1 cut(s) 664
BspFNI CGCG 1 cut(s) 540
BspLI GGNNCC 1 cut(s) 605
BspPI GGATC 1 cut(s) 524
BspT104I TTCGAA 1 cut(s) 375
BspT107I GGYRCC 1 cut(s) 603
BspTNI GGTCTC 2 cut(s) 220, 624
BsrGI TGTACA 1 cut(s) 96
BssECI CCNNGG 1 cut(s) 194
BssMI GATC 5 cut(s) 259, 309, 529, 535, 661
BssNI GRCGYC 1 cut(s) 604
BssT1I CCWWGG 1 cut(s) 194
Bst4CI ACNGT 5 cut(s) 130, 357, 439, 502, 701
BstACI GRCGYC 1 cut(s) 604
BstAUI TGTACA 1 cut(s) 96
BstBAI YACGTR 2 cut(s) 414, 443
BstBI TTCGAA 1 cut(s) 375
BstDEI CTNAG 2 cut(s) 146, 285
BstF5I GGATG 1 cut(s) 624
BstFNI CGCG 1 cut(s) 540
BstH2I RGCGCY 1 cut(s) 607
BstHHI GCGC 1 cut(s) 606
BstKTI GATC 5 cut(s) 262, 312, 532, 538, 664
BstMAI GTCTC 4 cut(s) 220, 491, 624, 648
BstMBI GATC 5 cut(s) 259, 309, 529, 535, 661
BstMCI CGRYCG 1 cut(s) 664
BstMWI GCNNNNNNNGC 2 cut(s) 454, 546
BstNSI RCATGY 2 cut(s) 472, 491
BstSLI GKGCMC 1 cut(s) 184
BstUI CGCG 1 cut(s) 540
BstV2I GAAGAC 2 cut(s) 131, 354
BstX2I RGATCY 1 cut(s) 259
BstYI RGATCY 1 cut(s) 259
Bsu15I ATCGAT 1 cut(s) 664
BsuRI GGCC 3 cut(s) 9, 193, 283
BsuTUI ATCGAT 1 cut(s) 664
BtsCI GGATG 1 cut(s) 624
BtsIMutI CAGTG 1 cut(s) 507
CaiI CAGNNNCTG 1 cut(s) 20
CfoI GCGC 1 cut(s) 606
Cfr13I GGNCC 2 cut(s) 7, 191
ClaI ATCGAT 1 cut(s) 664
Csp6I GTAC 4 cut(s) 97, 415, 440, 476
CviAII CATG 4 cut(s) 43, 177, 469, 488
CviJI RGCY 8 cut(s) 9, 30, 152, 187, 193, 283, 432, 549
CviKI_1 RGCY 8 cut(s) 9, 30, 152, 187, 193, 283, 432, 549
CviQI GTAC 4 cut(s) 97, 415, 440, 476
DdeI CTNAG 2 cut(s) 146, 285
DinI GGCGCC 1 cut(s) 605
DpnI GATC 5 cut(s) 261, 311, 531, 537, 663
DpnII GATC 5 cut(s) 259, 309, 529, 535, 661
Ecl136II GAGCTC 1 cut(s) 152
Eco130I CCWWGG 1 cut(s) 194
Eco147I AGGCCT 1 cut(s) 283
Eco24I GRGCYC 1 cut(s) 154
Eco31I GGTCTC 2 cut(s) 220, 624
Eco53kI GAGCTC 1 cut(s) 152
EcoICRI GAGCTC 1 cut(s) 152
EcoT14I CCWWGG 1 cut(s) 194
EcoT22I ATGCAT 2 cut(s) 470, 489
EcoT38I GRGCYC 1 cut(s) 154
EgeI GGCGCC 1 cut(s) 605
EheI GGCGCC 1 cut(s) 605
ErhI CCWWGG 1 cut(s) 194
FaeI CATG 4 cut(s) 46, 180, 472, 491
FatI CATG 4 cut(s) 42, 176, 468, 487
FokI GGATG 1 cut(s) 611
FriOI GRGCYC 1 cut(s) 154
GlaI GCGC 1 cut(s) 605
GsuI CTGGAG 1 cut(s) 630
HaeII RGCGCY 1 cut(s) 607
HaeIII GGCC 3 cut(s) 9, 193, 283
HhaI GCGC 1 cut(s) 606
Hin1I GRCGYC 1 cut(s) 604
Hin1II CATG 4 cut(s) 46, 180, 472, 491
Hin6I GCGC 1 cut(s) 604
HinP1I GCGC 1 cut(s) 604
HinfI GANTC 3 cut(s) 64, 372, 514
HphI GGTGA 3 cut(s) 73, 256, 319
Hpy166II GTNNAC 1 cut(s) 658
Hpy188I TCNGA 6 cut(s) 73, 147, 259, 529, 569, 629
Hpy188III TCNNGA 3 cut(s) 533, 647, 659
Hpy8I GTNNAC 1 cut(s) 658
HpyAV CCTTC 3 cut(s) 77, 134, 539
HpyCH4III ACNGT 5 cut(s) 130, 357, 439, 502, 701
HpyCH4IV ACGT 3 cut(s) 413, 442, 654
HpyCH4V TGCA 4 cut(s) 242, 332, 468, 487
HpyF10VI GCNNNNNNNGC 2 cut(s) 454, 546
HpyF3I CTNAG 2 cut(s) 146, 285
HpySE526I ACGT 3 cut(s) 413, 442, 654
Hsp92I GRCGYC 1 cut(s) 604
Hsp92II CATG 4 cut(s) 46, 180, 472, 491
HspAI GCGC 1 cut(s) 604
KasI GGCGCC 1 cut(s) 603
Kzo9I GATC 5 cut(s) 259, 309, 529, 535, 661
LpnPI CCDG 7 cut(s) 48, 196, 228, 346, 546, 589, 660
MaeII ACGT 3 cut(s) 413, 442, 654
MaeIII GTNAC 1 cut(s) 668
MalI GATC 5 cut(s) 261, 311, 531, 537, 663
MboI GATC 5 cut(s) 259, 309, 529, 535, 661
MboII GAAGA 3 cut(s) 136, 359, 509
MflI RGATCY 1 cut(s) 259
MhlI GDGCHC 3 cut(s) 154, 184, 459
MluCI AATT 6 cut(s) 200, 237, 271, 302, 333, 682
Mly113I GGCGCC 1 cut(s) 604
MnlI CCTC 4 cut(s) 219, 419, 606, 644
Mph1103I ATGCAT 2 cut(s) 470, 489
MroXI GAANNNNTTC 2 cut(s) 164, 654
MseI TTAA 1 cut(s) 558
MslI CAYNNNNRTG 1 cut(s) 492
Mva1269I GAATGC 1 cut(s) 320
MvnI CGCG 1 cut(s) 540
MwoI GCNNNNNNNGC 2 cut(s) 454, 546
NarI GGCGCC 1 cut(s) 604
NdeII GATC 5 cut(s) 259, 309, 529, 535, 661
NlaIII CATG 4 cut(s) 46, 180, 472, 491
NlaIV GGNNCC 1 cut(s) 605
NmuCI GTSAC 1 cut(s) 668
NsiI ATGCAT 2 cut(s) 470, 489
NspI RCATGY 2 cut(s) 472, 491
NspV TTCGAA 1 cut(s) 375
PceI AGGCCT 1 cut(s) 283
PcsI WCGNNNNNNNCGW 1 cut(s) 372
PctI GAATGC 1 cut(s) 320
PdmI GAANNNNTTC 2 cut(s) 164, 654
PfeI GAWTC 3 cut(s) 64, 372, 514
Ple19I CGATCG 1 cut(s) 664
PluTI GGCGCC 1 cut(s) 607
Ppu21I YACGTR 2 cut(s) 414, 443
Psp124BI GAGCTC 1 cut(s) 154
PspN4I GGNNCC 1 cut(s) 605
PspPI GGNCC 2 cut(s) 7, 191
PstNI CAGNNNCTG 1 cut(s) 20
PsuI RGATCY 1 cut(s) 259
PvuI CGATCG 1 cut(s) 664
RsaI GTAC 4 cut(s) 98, 416, 441, 477
RsaNI GTAC 4 cut(s) 97, 415, 440, 476
RseI CAYNNNNRTG 1 cut(s) 492
SacI GAGCTC 1 cut(s) 154
SaqAI TTAA 1 cut(s) 558
Sau3AI GATC 5 cut(s) 259, 309, 529, 535, 661
Sau96I GGNCC 2 cut(s) 7, 191
SduI GDGCHC 3 cut(s) 154, 184, 459
SfoI GGCGCC 1 cut(s) 605
SfuI TTCGAA 1 cut(s) 375
SmiMI CAYNNNNRTG 1 cut(s) 492
Sse9I AATT 6 cut(s) 200, 237, 271, 302, 333, 682
SseBI AGGCCT 1 cut(s) 283
SsiI CCGC 1 cut(s) 540
SspDI GGCGCC 1 cut(s) 603
SstI GAGCTC 1 cut(s) 154
StuI AGGCCT 1 cut(s) 283
StyI CCWWGG 1 cut(s) 194
TaaI ACNGT 5 cut(s) 130, 357, 439, 502, 701
TaiI ACGT 3 cut(s) 416, 445, 657
TaqI TCGA 6 cut(s) 232, 366, 375, 434, 453, 664
TasI AATT 6 cut(s) 200, 237, 271, 302, 333, 682
TatI WGTACW 2 cut(s) 96, 475
TfiI GAWTC 3 cut(s) 64, 372, 514
Tru1I TTAA 1 cut(s) 558
Tru9I TTAA 1 cut(s) 558
TscAI CASTG 1 cut(s) 507
TseFI GTSAC 1 cut(s) 668
Tsp45I GTSAC 1 cut(s) 668
TspDTI ATGAA 1 cut(s) 360
TspRI CASTG 1 cut(s) 507
XceI RCATGY 2 cut(s) 472, 491
XmnI GAANNNNTTC 2 cut(s) 164, 654
Zsp2I ATGCAT 2 cut(s) 470, 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.