Rmu_sc0002479.1_g000005

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002479.1
OG0007487
Physical Location & Seq
Reverse (-)
27042 .. 28181
1140 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1140 bp
atgcaactaactagtgctttgtgggcaagcaagaactttttactgcaacgtgtagggtatcagacagcaaatcaggaggactgcatgaatgatgcaaagttgctgtccttcgatgatgccagcctcgaaaccagtaccaataagctcattaccaaactaaaggtggatggaggtctttccctggatgttttggagaatgtgctgacaagtatgtctgatttagggcaaaaaactgctgacgcgatcttggatttcaagagtaagatgattgatatgaaggacgatagctataagcttgtggtgatggaatacttgagcagcagcctggagctgctggacttctttactgaattggaaaagtttctagactcggctcgtcggagccattcacatatcgaacttgctattgaacagtgtagccgcttggggggtaaacatggagtaggaagggaggaggccaagatgatttccaatatattgagtaacattatggcgtctaatgtcaatgaccactctggtgaaaagttggtcgaaaaggctcattgtttgcttgagaagcagaaagaaatgttttccaaatgtcgcattgaacaagagaagcttgggaaggataagaagagtaatgtttggaggaagctcttgagtcgtatattcatggttgccaagtggctcgttctcgttgttctaggggtgaaattggcctataccgcctataatataacatctggtggagatggtttgtctaatgtggttggagatttgaaaatcaaagcagcatgtttccttgatgtctctgttataacagaaatagtagattactggaatagctcccaccttgaagtatgtcaagatgattttgggctgaagaatgaagcaatgaacataatacaggaaggagctttacattccataagagaaatgggggaaataaaagatctcatcggtaaggtactaaattccattgaatctctccttgctttaagtacaaactttgcaaatggtggaaatgtagaagtggcaatcaagaatattaagctttacctgaagaagtttttggataaaattgaggctttggaggagaaaacaggggaatgcagctctagaattttgaaagcaaggcaagctgttctcatgtattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

379

Amino Acids

42.35

Weight (kDa)

5.91

Isoelectric Point (pI)

37.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 36 - 377 2.4e-27 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 800
AasI GACNNNNNNGTC 1 cut(s) 211
AccII CGCG 1 cut(s) 242
AciI CCGC 2 cut(s) 421, 708
AcsI RAATTY 2 cut(s) 955, 1104
AcuI CTGAAG 2 cut(s) 884, 1064
AcyI GRCGYC 1 cut(s) 494
AfaI GTAC 3 cut(s) 136, 951, 985
AfiI CCNNNNNNNGG 1 cut(s) 427
AflIII ACRYGT 1 cut(s) 49
AgsI TTSAA 7 cut(s) 256, 410, 590, 763, 839, 965, 1111
AhlI ACTAGT 1 cut(s) 11
AjnI CCWGG 2 cut(s) 180, 324
AjuI GAANNNNNNNTTGG 2 cut(s) 1037, 1069
AleI CACNNNNGTG 1 cut(s) 516
Alw26I GTCTC 1 cut(s) 796
AoxI GGCC 2 cut(s) 456, 699
ApeKI GCWGC 5 cut(s) 318, 321, 331, 773, 1095
ApoI RAATTY 2 cut(s) 955, 1104
ArsI GACNNNNNNTTYG 2 cut(s) 89, 121
Asp700I GAANNNNTTC 1 cut(s) 360
AsuHPI GGTGA 3 cut(s) 313, 530, 703
BarI GAAGNNNNNNTAC 2 cut(s) 885, 917
BbvI GCAGC 5 cut(s) 318, 330, 333, 785, 1107
BccI CCATC 3 cut(s) 161, 298, 728
BciT130I CCWGG 2 cut(s) 182, 326
BcoDI GTCTC 1 cut(s) 796
BcuI ACTAGT 1 cut(s) 11
BfaI CTAG 4 cut(s) 12, 365, 686, 1101
BglII AGATCT 1 cut(s) 934
BisI GCNGC 6 cut(s) 319, 322, 332, 421, 774, 1096
BlsI GCNGC 6 cut(s) 320, 323, 333, 422, 775, 1097
Bme1390I CCNGG 2 cut(s) 182, 326
BmiI GGNNCC 1 cut(s) 383
BmrFI CCNGG 2 cut(s) 182, 326
BmsI GCATC 2 cut(s) 82, 106
BpmI CTGGAG 1 cut(s) 347
BpuEI CTTGAG 3 cut(s) 334, 572, 661
BsaHI GRCGYC 1 cut(s) 494
BsaJI CCNNGG 1 cut(s) 180
Bsc4I CCNNNNNNNGG 1 cut(s) 427
Bse1I ACTGG 2 cut(s) 132, 824
Bse3DI GCAATG 1 cut(s) 882
BseBI CCWGG 2 cut(s) 182, 326
BseDI CCNNGG 1 cut(s) 180
BseGI GGATG 2 cut(s) 172, 190
BseLI CCNNNNNNNGG 1 cut(s) 427
BseMI GCAATG 1 cut(s) 882
BseNI ACTGG 2 cut(s) 132, 824
BseRI GAGGAG 2 cut(s) 467, 1091
BseXI GCAGC 5 cut(s) 318, 330, 333, 785, 1107
Bsh1236I CGCG 1 cut(s) 242
BshFI GGCC 2 cut(s) 458, 701
BslI CCNNNNNNNGG 1 cut(s) 427
BsmAI GTCTC 1 cut(s) 796
BsmI GAATGC 1 cut(s) 1097
BsnI GGCC 2 cut(s) 458, 701
Bsp143I GATC 2 cut(s) 243, 934
BspACI CCGC 2 cut(s) 421, 708
BspANI GGCC 2 cut(s) 458, 701
BspFNI CGCG 1 cut(s) 242
BspLI GGNNCC 1 cut(s) 383
BsrDI GCAATG 1 cut(s) 882
BsrI ACTGG 2 cut(s) 132, 824
BssECI CCNNGG 1 cut(s) 180
BssMI GATC 2 cut(s) 243, 934
BssNI GRCGYC 1 cut(s) 494
Bst2UI CCWGG 2 cut(s) 182, 326
Bst4CI ACNGT 1 cut(s) 414
Bst6I CTCTTC 1 cut(s) 611
BstACI GRCGYC 1 cut(s) 494
BstC8I GCNNGC 3 cut(s) 28, 121, 1122
BstF5I GGATG 2 cut(s) 172, 190
BstFNI CGCG 1 cut(s) 242
BstKTI GATC 2 cut(s) 246, 937
BstMAI GTCTC 1 cut(s) 796
BstMBI GATC 2 cut(s) 243, 934
BstMWI GCNNNNNNNGC 4 cut(s) 23, 556, 707, 1121
BstNI CCWGG 2 cut(s) 182, 326
BstNSI RCATGY 1 cut(s) 780
BstSCI CCNGG 2 cut(s) 180, 324
BstUI CGCG 1 cut(s) 242
BstV1I GCAGC 5 cut(s) 318, 330, 333, 785, 1107
BstX2I RGATCY 1 cut(s) 934
BstYI RGATCY 1 cut(s) 934
BsuRI GGCC 2 cut(s) 458, 701
BtsCI GGATG 2 cut(s) 172, 190
BtsIMutI CAGTG 1 cut(s) 419
Cac8I GCNNGC 3 cut(s) 28, 121, 1122
CseI GACGC 2 cut(s) 248, 483
Csp6I GTAC 3 cut(s) 135, 950, 984
CviAII CATG 5 cut(s) 85, 437, 655, 777, 1132
CviQI GTAC 3 cut(s) 135, 950, 984
DpnI GATC 2 cut(s) 245, 936
DpnII GATC 2 cut(s) 243, 934
DrdI GACNNNNNNGTC 1 cut(s) 211
DseDI GACNNNNNNGTC 1 cut(s) 211
Eam1104I CTCTTC 1 cut(s) 611
EarI CTCTTC 1 cut(s) 611
Eco57I CTGAAG 2 cut(s) 884, 1064
EcoRII CCWGG 2 cut(s) 180, 324
FaeI CATG 5 cut(s) 88, 440, 658, 780, 1135
FalI AAGNNNNNCTT 2 cut(s) 585, 617
FatI CATG 5 cut(s) 84, 436, 654, 776, 1131
Fnu4HI GCNGC 6 cut(s) 319, 322, 332, 421, 774, 1096
FokI GGATG 2 cut(s) 179, 197
Fsp4HI GCNGC 6 cut(s) 319, 322, 332, 421, 774, 1096
FspBI CTAG 4 cut(s) 12, 365, 686, 1101
GluI GCNGC 6 cut(s) 319, 322, 332, 421, 774, 1096
GsuI CTGGAG 1 cut(s) 347
HaeIII GGCC 2 cut(s) 458, 701
HgaI GACGC 2 cut(s) 248, 483
Hin1I GRCGYC 1 cut(s) 494
Hin1II CATG 5 cut(s) 88, 440, 658, 780, 1135
HindIII AAGCTT 3 cut(s) 293, 599, 1034
HinfI GANTC 3 cut(s) 368, 643, 965
HphI GGTGA 3 cut(s) 313, 530, 703
Hpy166II GTNNAC 1 cut(s) 434
Hpy188I TCNGA 3 cut(s) 63, 217, 381
Hpy188III TCNNGA 7 cut(s) 74, 256, 365, 640, 848, 1024, 1101
Hpy8I GTNNAC 1 cut(s) 434
Hpy99I CGWCG 1 cut(s) 381
HpyAV CCTTC 5 cut(s) 118, 271, 441, 601, 887
HpyCH4III ACNGT 1 cut(s) 414
HpyCH4IV ACGT 1 cut(s) 49
HpyCH4V TGCA 6 cut(s) 4, 46, 84, 95, 995, 1095
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 556, 707, 1121
HpySE526I ACGT 1 cut(s) 49
Hsp92I GRCGYC 1 cut(s) 494
Hsp92II CATG 5 cut(s) 88, 440, 658, 780, 1135
Kzo9I GATC 2 cut(s) 243, 934
LmnI GCTCC 4 cut(s) 328, 381, 833, 896
Lsp1109I GCAGC 5 cut(s) 318, 330, 333, 785, 1107
LweI GCATC 2 cut(s) 82, 106
MaeI CTAG 4 cut(s) 12, 365, 686, 1101
MaeII ACGT 1 cut(s) 49
MaeIII GTNAC 1 cut(s) 482
MalI GATC 2 cut(s) 245, 936
MboI GATC 2 cut(s) 243, 934
MboII GAAGA 3 cut(s) 628, 877, 1057
MflI RGATCY 1 cut(s) 934
MluCI AATT 5 cut(s) 350, 695, 955, 1062, 1104
MlyI GAGTC 2 cut(s) 362, 652
MmeI TCCRAC 2 cut(s) 359, 733
MnlI CCTC 8 cut(s) 70, 134, 164, 445, 448, 624, 1060, 1069
MroXI GAANNNNTTC 1 cut(s) 360
MseI TTAA 3 cut(s) 980, 1032, 1138
MslI CAYNNNNRTG 1 cut(s) 516
MspR9I CCNGG 2 cut(s) 182, 326
Mva1269I GAATGC 1 cut(s) 1097
MvaI CCWGG 2 cut(s) 182, 326
MvnI CGCG 1 cut(s) 242
MwoI GCNNNNNNNGC 4 cut(s) 23, 556, 707, 1121
NdeII GATC 2 cut(s) 243, 934
NlaIII CATG 5 cut(s) 88, 440, 658, 780, 1135
NlaIV GGNNCC 1 cut(s) 383
NmeAIII GCCGAG 1 cut(s) 350
NspI RCATGY 1 cut(s) 780
OliI CACNNNNGTG 1 cut(s) 516
PctI GAATGC 1 cut(s) 1097
PdmI GAANNNNTTC 1 cut(s) 360
PfeI GAWTC 1 cut(s) 965
PkrI GCNGC 6 cut(s) 320, 323, 333, 422, 775, 1097
PleI GAGTC 2 cut(s) 362, 651
PpsI GAGTC 2 cut(s) 362, 651
PsiI TTATAA 1 cut(s) 800
Psp6I CCWGG 2 cut(s) 180, 324
PspGI CCWGG 2 cut(s) 180, 324
PspN4I GGNNCC 1 cut(s) 383
PsuI RGATCY 1 cut(s) 934
RsaI GTAC 3 cut(s) 136, 951, 985
RsaNI GTAC 3 cut(s) 135, 950, 984
RseI CAYNNNNRTG 1 cut(s) 516
SaqAI TTAA 3 cut(s) 980, 1032, 1138
SatI GCNGC 6 cut(s) 319, 322, 332, 421, 774, 1096
Sau3AI GATC 2 cut(s) 243, 934
SchI GAGTC 2 cut(s) 362, 652
ScrFI CCNGG 2 cut(s) 182, 326
SfaNI GCATC 2 cut(s) 82, 106
SmiMI CAYNNNNRTG 1 cut(s) 516
SmlI CTYRAG 3 cut(s) 313, 551, 640
SmoI CTYRAG 3 cut(s) 313, 551, 640
SpeI ACTAGT 1 cut(s) 11
Sse9I AATT 5 cut(s) 350, 695, 955, 1062, 1104
SsiI CCGC 2 cut(s) 421, 708
SspI AATATT 1 cut(s) 1030
SspMI CTAG 4 cut(s) 12, 365, 686, 1101
StyD4I CCNGG 2 cut(s) 180, 324
TaaI ACNGT 1 cut(s) 414
TaiI ACGT 1 cut(s) 52
TaqI TCGA 4 cut(s) 111, 126, 396, 531
TasI AATT 5 cut(s) 350, 695, 955, 1062, 1104
TatI WGTACW 1 cut(s) 983
TauI GCSGC 1 cut(s) 423
TfiI GAWTC 1 cut(s) 965
Tru1I TTAA 3 cut(s) 980, 1032, 1138
Tru9I TTAA 3 cut(s) 980, 1032, 1138
TscAI CASTG 1 cut(s) 419
TseI GCWGC 5 cut(s) 318, 321, 331, 773, 1095
TspDTI ATGAA 5 cut(s) 101, 290, 643, 885, 893
TspRI CASTG 1 cut(s) 419
XapI RAATTY 2 cut(s) 955, 1104
XbaI TCTAGA 2 cut(s) 364, 1100
XceI RCATGY 1 cut(s) 780
XcmI CCANNNNNNNNNTGG 2 cut(s) 160, 916
XmnI GAANNNNTTC 1 cut(s) 360
XspI CTAG 4 cut(s) 12, 365, 686, 1101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.