Rmu_sc0002506.1_g000034

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002506.1
OG0004044
Physical Location & Seq
Forward (+)
138039 .. 150510
12472 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 6147 bp
atgatttcgaaatatgtcaccggaaaatttggccggaaaaagtcgttggaggtgacgtggccctctgacgctgacgtgttaatggcttctgggctgacgtcaacgggcttctgggctgacatcagtgggcttcatgtagaccggctaaggatgtcaggggatctactggcccgttctaggtctctacggccggttcctaggatcttttggctggttcccggattttgggatagggctgcagcttctgacaaaagacggcgccggtttcgggggtggcgccgccggttctggactcctggagttgagggtttcaaggtgggcgtcctccctgttaaactagttaactcgtacccgcttcaatttcaatttacggatttgacctccacctcctcctcctccgtctctttgtttccttcccaacttcatcactccccagaaaactgtcctccggcgaacctgaaactcccacatgcttcgtcctcgtcctccatggactccattctaaactcagcgatcgaagaaatctgctcgtatctccaaaacggcctctctctccagaccctctggtcccgactctcctcctcctccaacctcgacctctctcccacactcaaacaatccctatgggacgccctccgctccgtccccactctcaaattccgcgcccaaaatgaccgttacgcccccaccgacccttccattcactccttccaagacgccgagaagctcaatttgaagctcgtcgccgatgaggccctccggaacaacttcatgggcctctacaatgtcgactccgtcaatgccaacctgtctgctgttcaacgccacactctcgaacgcgtcgtcatggctagggggaatgggattacacaaactcagcttgctaaggagcttggcattgaggggagaaactttcattatgctgtgaagaacctcgaatgtcaaggtttacttgtacggcaatctgcgctgttaaggacaaaagaagctggtgatgacggggagccgaggaacaatccgagtgtgacaacgaccaatatgttgtacttgtatcgacatgcaaaacatttaagcgcgcaacaaaagattgagatcacaaaggaagagagggctaaggagagttttgtaaatgtaaaagaaagccctgcaagtggagatggttttgctggaaatgtgctcgtaaaggactatctaccggcaatgaaagcagtctgtaataaacttgaagaagccaatggcaaggttcttgtcgtttcggatattaagaaggaacttggctacagtaaaacatcagggcatagagcttggagaaatatttgtcagaggttgaaagctgctcatcttgtggaggtgtttgacgctaaagtgaatgggaaggttgagagttgcctgcgttttgtagaaaattcttctccaatgaaagttgagccaagagaagttgtacatgtagatgaggattttgttgaagaacaacaagtgaaattcggaaagaaatgtaaaatcagtgaccagcttgtagagcttcctattgagcatcaaatttatgaattaatagatgcggcaggatcagaggggttgaccaggaatgaggttatggagaggcttggaattgacaacaagaaaaattatacccgctttgttagtatgtgctctagatttgatatgagtctgcaaccagaaatgcacaaaaaagctgtggcatatcgatttcggacatctgggaaacacaggtctgaatcaactaatgcatttcttcgaaaatcaaaagatgccaatgacagtaaaatttttagtctaaatgttgggagtgtagatgctctgagatcagatcaatttcaaactggctcagtgtcagattgttggtctctgaacagtgatactgctggccctgaaaatatgaataacatagagactactactgacccttctgctggatctcttgggcgtaatgagtttgacgatatgcccgaaacttcccaacagttacttcttggaccaaaagatactacatctgattctcaagttagtttagtgagcgcaggagtggaaccaaatggcgccttatcagaaataccagctgctttgtcaaagccacttgctaaaggctcagatccaaggtacccatgtatgtctttgaccgtggataacactcgaagggaaaagaggatacttgaacggctagagggtgagaagtttattttaagagctgagctgtatagatggcttgtgagcttagagacggacaagtgcacagcaactgacaggaaaacaattgaccgaattttacaaaaacttcaacagttagggcactgcaagtgtatagatattagcgtccctgtcgtcacaaatttgggccgtacccgaaccactgttgtggttctgcacccatctgttcagagtttaactcctgaacttgtttctgaaattcatgatgcttggaggtcttttgagattcaaagtcgtggcaagtgctcatctcggtggcgggagaagaacagtggatcagttcctgtactagaagatgttcagagaactcagacccatgttagtgcacagcgacaaacactgagttcagaaaccatgcgtgctaatggatttattctggcaaaaatggttcgtgcaaaattgctgcatagtttcctgtgggattacctgtatggttcgtccggctctaatgatgctttatcatttggaaaagatgttattgagccaagagatcctcacggtactagaaaattgttttctctagaagcaacaatgaaggctattccagttgagttgtttctacaagttgcgggatccaccaaaaactttgaggatatgattgagaaatgtaagaggggtttatgtctctctgatctctctcgtgaagaatacaagtctctgatggatacccatgcaactggaaggctctcattggtgattgacattttacgacgattgaagctgattcgaatggtttgtgatcaccactcagaaaatggattccaagttccccctcccatgatttctgcatatgcattggaacttaagccttatgtagaggaacctgtttcaaaagatgcgatatctttgagttttggatcacgtgatcttcgtccacgaatcagacatgatttttccctagcaagcagagaagctgttgatgagtattggcaaactttagagtattgttatgctgctgcggacccaagagctgccttacttgcatttcctgggtcttgtgttcatgaggtgtcccatcacaggtcatggactaaacttggagttatgacagctgctcagcgtgatgagctcttaaagcgtgttgtgaaggatgatccaagtgaaaagctttcattcaaagagtgtggaaaaattgcgaaggatctcaatttgaccctggagcaggtgcttcgtgtgtattataataagcggcgtcaacatcttgatgggttgcaaaataacacagatgaagttcaatctaaaaaacgtagacgtcgtaaaaggaagagatcttctgaatctaggtcagtagattttactgaaaattatgaggtaactggacagctggaagagcagatacatcccacagtatctgatactgtggaacaattagaagaactaaatctcttggtcacttcttatgagcatgattcacatttgcaagcattggacaattgtctagaaactgggcaggaacctgaaccaaatgaagataatgaggggtgccattcaattattaccaagggttccttttcaaagttgaagccactacgtccttctaaaagaaacaagttgcactcaacacgtcaacgaaggttttcatggacagaggaggctgataggcagttgattatccaatatgtaagacatcgtgcaactcttggggcaaagattcaccggataaattgggcttcagttactgacctcccagcacctcctattgcctgcatgaaaagaatggcatctctgaaaagtaacagaaagttcagattagctgtaatgaggctttgtaacattctcagtgaacgatatgcaaagatcttggaaaagactcagaacaggtcactgaacaaagatgattgcaatctgcttttgcgagattcaataggtgaaggtcatgacagcaaacttcccaatatcagtgatcataatccagagacaggtcttcaggaagaaccatgggatgattttgatgacgtttatgttaaaaagtcccttgaggaggtacttcaccacaagaggttggccaaatttgatgcctccaaaagagttggatctaccggtgaggactggtcggatcttaatacaagtgaatgtgatccccaggaatctgaattaattgtatcaactgctccatatgaagatgttcagaatcatggtagcagagaaaaaatttcggcacgaagatcttgctatcaacaccttaatgaaaaatactttaagcttttgcatggagttgatgtcagtacacaagtctacaaatcattagctgtttctaatgctgtagagctgtttaagcttgttttcctcagtacctcaacagcaccagaagtgccaaatctgcttgcaggcattctacggcggtactcagagtgtgatctattcgcagctttcaactatcttagagataaaaagttcatggttggaggtaatggcagtcaaaaattttctctctcacaacagttcttgcataatacttccgcatcaccatttccaattaattctgggaagagagctactaaatttgcccattggctctacgaaaaagataaagatcttatggaagggggcatagatctctccacagatttacagtgtggggacatattccatttgtttgctctagtttcttcaggtgaaatgtcaatttctccatgcttgccagatgagggtgtgggagaggctgaagaatcaagaagctcaaaacgtaaagcagatatcaatgaacttttagatgatgaaaaaactaaaaagctgaaatcatttgtggctgcagaaggtgaaatcatttcccgtcgagaaaaaggtttcccgggtatcacggtatctgtaagtcgtgcagagttttctatagccaattctatagatttgttcaaggaagacgctcctattggtgacaaacattttgggggaaatcatcaactagaatgtacatctgatcagagtagcttatcacattctgactgtatgaaagaaatcttcaattcaggcagtactgtccctgtattggagattggttgcgagtcaccttgggaaggtatggtggcttatgccgaacatttgttgccattacatcctgctcaagatcagtcaagtcccatccagcctgaggtctttaggactgtttacattgctattcagaaagctggcgatcaaggtctgagcatagaagaagtttcccgaataatgaatatcccaggagaaaagatgactgatcttatcattgatgtgcttcagacatttgaacgagtcttaaaggtcaatgcttatgactctattcgtgttgtggattctttgtatcgtggcaagtactttatgacttcagtgtctggaagtaatcaaaaactcgaaccaccttcctggagaaagccactaggcgatgacggccatatactcttcctttcagaaaatcgtgataatggtgctgctccttcagagagggagataaatgctgatgtccataaattgacaattcttaattttcctgaagaggttgacgagccctcatatgtaaaacaaattagcagtgttcctgaaagctatagggaaggaaaaggaggagatgctgaggatgaatcttctaggtcctctaatgatagactatgcatgcctatatttccatggataaatggtgatgggagcactaacaagattgtctacaagggactcaggcgacgtgtgcttggggttgtgatgcaaaatccagctatattagaggatgaaattattcgccggatggatgtattgaatccccagagctgtagaaaattgttggaattgatggttttggataaccatcttcatgtgaggaagatgcatcaaactgcgtgcattgggcccccaccaattttaggtaccctccttggaagcagcttcaaaccgtcaaagctggtttgtcgcgagcactattttgcaaatcccatgagtacatccttgttgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B-block_TFIIIC

Protein Analysis

2048

Amino Acids

230.66

Weight (kDa)

7.55

Isoelectric Point (pI)

52.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_GTF3C1_N PF23704 164 - 261 8.3e-34 General transcription factor 3C polypeptide 1, winged-helix domain
B-block_TFIIIC PF04182 272 - 355 1.3e-12 B-block binding subunit of TFIIIC
DUF7599 PF24538 395 - 477 7.5e-36 Domain of unknown function (DUF7599)
DUF7646 PF24657 496 - 579 2.3e-37 Domain of unknown function (DUF7646)
WHD_GTF3C1 PF24101 712 - 821 3.4e-40 GTF3C1, extended winged-helix domain
DUF7647 PF24658 907 - 1086 1.7e-82 Domain of unknown function (DUF7647)
DUF7645 PF24655 1089 - 1144 9.7e-30 Domain of unknown function (DUF7645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3408
AarI CACCTGC 1 cut(s) 3379
AasI GACNNNNNNGTC 2 cut(s) 565, 5530
AatII GACGTC 2 cut(s) 101, 3481
Acc36I ACCTGC 1 cut(s) 3379
Acc65I GGTACC 2 cut(s) 2118, 6059
AccB1I GGYRCC 6 cut(s) 258, 276, 2057, 2118, 3708, 6059
AccBSI CCGCTC 1 cut(s) 639
AccI GTMKAC 5 cut(s) 138, 789, 3475, 4461, 5862
AccII CGCG 4 cut(s) 663, 840, 1076, 6105
AccIII TCCGGA 1 cut(s) 759
AcoI YGGCCR 4 cut(s) 31, 188, 4230, 5590
AcuI CTGAAG 8 cut(s) 3882, 4136, 4821, 4911, 5423, 5511, 5622, 5712
AcvI CACGTG 1 cut(s) 3089
AcyI GRCGYC 9 cut(s) 98, 259, 277, 321, 630, 717, 2058, 3418, 3478
AflII CTTAAG 1 cut(s) 3029
AflIII ACRYGT 5 cut(s) 75, 838, 1444, 3788, 5881
AgeI ACCGGT 1 cut(s) 4265
AhlI ACTAGT 1 cut(s) 337
AjiI CACGTC 4 cut(s) 57, 76, 3791, 5882
AjnI CCWGG 7 cut(s) 295, 1580, 3214, 3381, 4308, 5401, 5564
AjuI GAANNNNNNNTTGG 4 cut(s) 29, 61, 1971, 2003
AleI CACNNNNGTG 1 cut(s) 2372
AloI GAACNNNNNNTCC 2 cut(s) 2976, 3008
Alw21I GWGCWC 8 cut(s) 1179, 1652, 2252, 2474, 2554, 3297, 5849, 6111
Alw26I GTCTC 8 cut(s) 186, 406, 1869, 1904, 2231, 2855, 2886, 4136
Alw44I GTGCAC 2 cut(s) 2248, 2550
AlwNI CAGNNNCTG 6 cut(s) 245, 2258, 2510, 3578, 3905, 5534
Ama87I CYCGRG 1 cut(s) 5017
Aor13HI TCCGGA 1 cut(s) 759
ApaI GGGCCC 1 cut(s) 6045
ApaLI GTGCAC 2 cut(s) 2248, 2550
ArsI GACNNNNNNTTYG 4 cut(s) 828, 860, 5093, 5125
AseI ATTAAT 3 cut(s) 1550, 4322, 4704
AsiGI ACCGGT 1 cut(s) 4265
Asp700I GAANNNNTTC 5 cut(s) 767, 2523, 3802, 4350, 5931
Asp718I GGTACC 2 cut(s) 2118, 6059
AspA2I CCTAGG 1 cut(s) 197
AspLEI GCGC 8 cut(s) 261, 279, 665, 970, 1076, 1078, 2039, 2060
AsuC2I CCSGG 3 cut(s) 219, 5018, 5019
AsuII TTCGAA 3 cut(s) 8, 1756, 2953
AvaI CYCGRG 1 cut(s) 5017
AvaII GGWCC 4 cut(s) 567, 1994, 3187, 5790
AvrII CCTAGG 1 cut(s) 197
AxyI CCTNAGG 1 cut(s) 5313
BaeGI GKGCMC 4 cut(s) 2252, 2310, 2554, 6045
BaeI ACNNNNGTAYC 2 cut(s) 2110, 2143
BalI TGGCCA 1 cut(s) 4232
BamHI GGATCC 1 cut(s) 2798
BanI GGYRCC 6 cut(s) 258, 276, 2057, 2118, 3708, 6059
BanII GRGCYC 3 cut(s) 3297, 5709, 6045
BarI GAAGNNNNNNTAC 2 cut(s) 3546, 3578
BauI CACGAG 1 cut(s) 2865
BbrPI CACGTG 1 cut(s) 3089
BbsI GAAGAC 2 cut(s) 4142, 5091
Bbv12I GWGCWC 8 cut(s) 1179, 1652, 2252, 2474, 2554, 3297, 5849, 6111
BbvCI CCTCAGC 1 cut(s) 5772
BceAI ACGGC 8 cut(s) 203, 272, 559, 974, 2192, 2340, 4580, 5605
BcgI CGANNNNNNTGC 4 cut(s) 3377, 3411, 4377, 4411
BciT130I CCWGG 7 cut(s) 297, 1582, 3216, 3383, 4310, 5403, 5566
BciVI GTATCC 2 cut(s) 2160, 2884
BclI TGATCA 3 cut(s) 2965, 4129, 5143
BcnI CCSGG 3 cut(s) 219, 5018, 5019
BcoDI GTCTC 8 cut(s) 186, 406, 1869, 1904, 2231, 2855, 2886, 4136
BcuI ACTAGT 1 cut(s) 337
BfmI CTRYAG 8 cut(s) 237, 1279, 4488, 4977, 5055, 5067, 5746, 5965
BfoI RGCGCY 3 cut(s) 262, 280, 2061
BfrI CTTAAG 1 cut(s) 3029
BfuAI ACCTGC 1 cut(s) 3379
BfuI GTATCC 2 cut(s) 2160, 2884
BglI GCCNNNNNGGC 1 cut(s) 752
BglII AGATCT 5 cut(s) 3494, 4023, 4391, 4759, 4780
BlnI CCTAGG 1 cut(s) 197
BlpI GCTNAGC 2 cut(s) 2208, 3282
BmcAI AGTACT 2 cut(s) 5200, 5516
Bme18I GGWCC 4 cut(s) 567, 1994, 3187, 5790
BmeT110I CYCGRG 1 cut(s) 5017
BmgBI CACGTC 4 cut(s) 57, 76, 3791, 5882
BmrI ACTGGG 1 cut(s) 3681
BmuI ACTGGG 1 cut(s) 3681
BoxI GACNNNNGTC 1 cut(s) 3660
BpiI GAAGAC 2 cut(s) 4142, 5091
BplI GAGNNNNNCTC 2 cut(s) 2389, 2421
BpmI CTGGAG 4 cut(s) 318, 539, 3404, 5587
Bpu10I CCTNAGC 4 cut(s) 146, 885, 1113, 5772
Bpu1102I GCTNAGC 2 cut(s) 2208, 3282
Bpu14I TTCGAA 3 cut(s) 8, 1756, 2953
BpuEI CTTGAG 3 cut(s) 2004, 4223, 5271
BpuMI CCSGG 3 cut(s) 219, 5018, 5019
Bsa29I ATCGAT 1 cut(s) 1705
BsaAI YACGTR 1 cut(s) 3089
BsaBI GATNNNNATC 5 cut(s) 1091, 4068, 4134, 4758, 6000
BsaHI GRCGYC 9 cut(s) 98, 259, 277, 321, 630, 717, 2058, 3418, 3478
BsaI GGTCTC 2 cut(s) 186, 1869
BsaWI WCCGGW 4 cut(s) 20, 759, 3882, 4265
BsaXI ACNNNNNCTCC 4 cut(s) 41, 71, 4431, 4461
Bse118I RCCGGY 6 cut(s) 141, 190, 261, 282, 1195, 4265
Bse1I ACTGG 7 cut(s) 171, 1846, 2771, 2908, 3547, 3676, 4280
Bse21I CCTNAGG 1 cut(s) 5313
Bse3DI GCAATG 2 cut(s) 1206, 5334
Bse8I GATNNNNATC 5 cut(s) 1091, 4068, 4134, 4758, 6000
BseAI TCCGGA 1 cut(s) 759
BseBI CCWGG 7 cut(s) 297, 1582, 3216, 3383, 4310, 5403, 5566
BseCI ATCGAT 1 cut(s) 1705
BseJI GATNNNNATC 5 cut(s) 1091, 4068, 4134, 4758, 6000
BseMI GCAATG 2 cut(s) 1206, 5334
BseNI ACTGG 7 cut(s) 171, 1846, 2771, 2908, 3547, 3676, 4280
BsePI GCGCGC 1 cut(s) 1074
BseRI GAGGAG 9 cut(s) 379, 382, 385, 568, 571, 574, 3830, 4220, 5778
BseSI GKGCMC 4 cut(s) 2252, 2310, 2554, 6045
BseX3I CGGCCG 1 cut(s) 188
BseYI CCCAGC 1 cut(s) 3913
BsgI GTGCAG 2 cut(s) 2366, 5064
Bsh1236I CGCG 4 cut(s) 663, 840, 1076, 6105
Bsh1285I CGRYCG 2 cut(s) 191, 516
BshNI GGYRCC 6 cut(s) 258, 276, 2057, 2118, 3708, 6059
BshTI ACCGGT 1 cut(s) 4265
BshVI ATCGAT 1 cut(s) 1705
BsiEI CGRYCG 2 cut(s) 191, 516
BsiHKAI GWGCWC 8 cut(s) 1179, 1652, 2252, 2474, 2554, 3297, 5849, 6111
BsiHKCI CYCGRG 1 cut(s) 5017
BsmAI GTCTC 8 cut(s) 186, 406, 1869, 1904, 2231, 2855, 2886, 4136
BsmBI CGTCTC 2 cut(s) 406, 2231
BsmI GAATGC 1 cut(s) 4557
Bso31I GGTCTC 2 cut(s) 186, 1869
BsoBI CYCGRG 1 cut(s) 5017
Bsp119I TTCGAA 3 cut(s) 8, 1756, 2953
Bsp120I GGGCCC 1 cut(s) 6041
Bsp13I TCCGGA 1 cut(s) 759
Bsp1407I TGTACA 2 cut(s) 1441, 5135
Bsp1720I GCTNAGC 2 cut(s) 2208, 3282
Bsp19I CCATGG 3 cut(s) 489, 4163, 5825
Bsp68I TCGCGA 1 cut(s) 6105
BspDI ATCGAT 1 cut(s) 1705
BspEI TCCGGA 1 cut(s) 759
BspFNI CGCG 4 cut(s) 663, 840, 1076, 6105
BspHI TCATGA 3 cut(s) 2428, 3229, 4102
BspMAI CTGCAG 2 cut(s) 241, 4981
BspMI ACCTGC 1 cut(s) 3379
BspQI GCTCTTC 1 cut(s) 3549
BspT104I TTCGAA 3 cut(s) 8, 1756, 2953
BspT107I GGYRCC 6 cut(s) 258, 276, 2057, 2118, 3708, 6059
BspTI CTTAAG 1 cut(s) 3029
BspTNI GGTCTC 2 cut(s) 186, 1869
BsrBI CCGCTC 1 cut(s) 639
BsrDI GCAATG 2 cut(s) 1206, 5334
BsrFI RCCGGY 6 cut(s) 141, 190, 261, 282, 1195, 4265
BsrGI TGTACA 2 cut(s) 1441, 5135
BsrI ACTGG 7 cut(s) 171, 1846, 2771, 2908, 3547, 3676, 4280
BssAI RCCGGY 6 cut(s) 141, 190, 261, 282, 1195, 4265
BssHII GCGCGC 1 cut(s) 1074
BssNI GRCGYC 9 cut(s) 98, 259, 277, 321, 630, 717, 2058, 3418, 3478
BssSI CACGAG 1 cut(s) 2865
BssT1I CCWWGG 8 cut(s) 197, 489, 2114, 3726, 4163, 5234, 5825, 6067
Bst2BI CACGAG 1 cut(s) 2865
Bst2UI CCWGG 7 cut(s) 297, 1582, 3216, 3383, 4310, 5403, 5566
Bst6I CTCTTC 6 cut(s) 1098, 3485, 3549, 4709, 5606, 5688
BstACI GRCGYC 9 cut(s) 98, 259, 277, 321, 630, 717, 2058, 3418, 3478
BstAFI CTTAAG 1 cut(s) 3029
BstAUI TGTACA 2 cut(s) 1441, 5135
BstBAI YACGTR 1 cut(s) 3089
BstBI TTCGAA 3 cut(s) 8, 1756, 2953
BstDSI CCRYGG 4 cut(s) 489, 2139, 4163, 5825
BstENI CCTNNNNNAGG 3 cut(s) 3386, 4205, 5238
BstFNI CGCG 4 cut(s) 663, 840, 1076, 6105
BstH2I RGCGCY 3 cut(s) 262, 280, 2061
BstHHI GCGC 8 cut(s) 261, 279, 665, 970, 1076, 1078, 2039, 2060
BstMAI GTCTC 8 cut(s) 186, 406, 1869, 1904, 2231, 2855, 2886, 4136
BstMCI CGRYCG 2 cut(s) 191, 516
BstNI CCWGG 7 cut(s) 297, 1582, 3216, 3383, 4310, 5403, 5566
BstNSI RCATGY 4 cut(s) 473, 1061, 1448, 5815
BstPAI GACNNNNGTC 1 cut(s) 3660
BstSFI CTRYAG 8 cut(s) 237, 1279, 4488, 4977, 5055, 5067, 5746, 5965
BstSLI GKGCMC 4 cut(s) 2252, 2310, 2554, 6045
BstUI CGCG 4 cut(s) 663, 840, 1076, 6105
BstV2I GAAGAC 2 cut(s) 4142, 5091
BstXI CCANNNNNNTGG 3 cut(s) 2374, 2903, 5565
BstZI CGGCCG 1 cut(s) 188
Bsu15I ATCGAT 1 cut(s) 1705
Bsu36I CCTNAGG 1 cut(s) 5313
BsuI GTATCC 2 cut(s) 2160, 2884
BsuTUI ATCGAT 1 cut(s) 1705
BtgI CCRYGG 4 cut(s) 489, 2139, 4163, 5825
BtgZI GCGATG 1 cut(s) 5598
BtrI CACGTC 4 cut(s) 57, 76, 3791, 5882
BtsI GCAGTG 2 cut(s) 2308, 5737
BtuMI TCGCGA 1 cut(s) 6105
BveI ACCTGC 1 cut(s) 3379
CaiI CAGNNNCTG 6 cut(s) 245, 2258, 2510, 3578, 3905, 5534
CciI TCATGA 3 cut(s) 2428, 3229, 4102
CfoI GCGC 8 cut(s) 261, 279, 665, 970, 1076, 1078, 2039, 2060
Cfr10I RCCGGY 6 cut(s) 141, 190, 261, 282, 1195, 4265
Cfr9I CCCGGG 1 cut(s) 5017
ClaI ATCGAT 1 cut(s) 1705
CseI GACGC 9 cut(s) 77, 310, 638, 725, 829, 1367, 2320, 3407, 5096
CspAI ACCGGT 1 cut(s) 4265
CspCI CAANNNNNGTGG 2 cut(s) 3331, 3366
DinI GGCGCC 3 cut(s) 260, 278, 2059
DrdI GACNNNNNNGTC 2 cut(s) 565, 5530
DseDI GACNNNNNNGTC 2 cut(s) 565, 5530
EaeI YGGCCR 4 cut(s) 31, 188, 4230, 5590
EagI CGGCCG 1 cut(s) 188
Eam1104I CTCTTC 6 cut(s) 1098, 3485, 3549, 4709, 5606, 5688
EarI CTCTTC 6 cut(s) 1098, 3485, 3549, 4709, 5606, 5688
Ecl136II GAGCTC 1 cut(s) 3295
EclXI CGGCCG 1 cut(s) 188
Eco130I CCWWGG 8 cut(s) 197, 489, 2114, 3726, 4163, 5234, 5825, 6067
Eco24I GRGCYC 3 cut(s) 3297, 5709, 6045
Eco31I GGTCTC 2 cut(s) 186, 1869
Eco32I GATATC 2 cut(s) 3069, 4924
Eco47I GGWCC 4 cut(s) 567, 1994, 3187, 5790
Eco52I CGGCCG 1 cut(s) 188
Eco53kI GAGCTC 1 cut(s) 3295
Eco57I CTGAAG 8 cut(s) 3882, 4136, 4821, 4911, 5423, 5511, 5622, 5712
Eco72I CACGTG 1 cut(s) 3089
Eco81I CCTNAGG 1 cut(s) 5313
Eco88I CYCGRG 1 cut(s) 5017
EcoICRI GAGCTC 1 cut(s) 3295
EcoNI CCTNNNNNAGG 3 cut(s) 3386, 4205, 5238
EcoO109I RGGNCCY 3 cut(s) 754, 5790, 6042
EcoRII CCWGG 7 cut(s) 295, 1580, 3214, 3381, 4308, 5401, 5564
EcoRV GATATC 2 cut(s) 3069, 4924
EcoT14I CCWWGG 8 cut(s) 197, 489, 2114, 3726, 4163, 5234, 5825, 6067
EcoT22I ATGCAT 4 cut(s) 1750, 3022, 5813, 6024
EcoT38I GRGCYC 3 cut(s) 3297, 5709, 6045
EgeI GGCGCC 3 cut(s) 260, 278, 2059
EheI GGCGCC 3 cut(s) 260, 278, 2059
ErhI CCWWGG 8 cut(s) 197, 489, 2114, 3726, 4163, 5234, 5825, 6067
Esp3I CGTCTC 2 cut(s) 406, 2231
FalI AAGNNNNNCTT 4 cut(s) 936, 968, 3719, 3751
FauI CCCGC 4 cut(s) 360, 1640, 2478, 2788
FauNDI CATATG 3 cut(s) 3016, 4342, 5713
FbaI TGATCA 3 cut(s) 2965, 4129, 5143
FblI GTMKAC 5 cut(s) 138, 789, 3475, 4461, 5862
FriOI GRGCYC 3 cut(s) 3297, 5709, 6045
GlaI GCGC 8 cut(s) 260, 278, 664, 969, 1075, 1077, 2038, 2059
GsaI CCCAGC 1 cut(s) 3917
GsuI CTGGAG 4 cut(s) 318, 539, 3404, 5587
HaeII RGCGCY 3 cut(s) 262, 280, 2061
HgaI GACGC 9 cut(s) 77, 310, 638, 725, 829, 1367, 2320, 3407, 5096
HhaI GCGC 8 cut(s) 261, 279, 665, 970, 1076, 1078, 2039, 2060
Hin1I GRCGYC 9 cut(s) 98, 259, 277, 321, 630, 717, 2058, 3418, 3478
Hin6I GCGC 8 cut(s) 259, 277, 663, 968, 1074, 1076, 2037, 2058
HinP1I GCGC 8 cut(s) 259, 277, 663, 968, 1074, 1076, 2037, 2058
HincII GTYRAC 7 cut(s) 102, 343, 790, 1578, 3422, 3794, 5701
HindII GTYRAC 7 cut(s) 102, 343, 790, 1578, 3422, 3794, 5701
HindIII AAGCTT 3 cut(s) 3332, 4427, 4502
HpaI GTTAAC 1 cut(s) 343
Hpy99I CGWCG 6 cut(s) 746, 845, 2940, 3483, 5004, 5883
Hsp92I GRCGYC 9 cut(s) 98, 259, 277, 321, 630, 717, 2058, 3418, 3478
HspAI GCGC 8 cut(s) 259, 277, 663, 968, 1074, 1076, 2037, 2058
KasI GGCGCC 3 cut(s) 258, 276, 2057
Kpn2I TCCGGA 1 cut(s) 759
KpnI GGTACC 2 cut(s) 2122, 6063
Ksp22I TGATCA 3 cut(s) 2965, 4129, 5143
KspAI GTTAAC 1 cut(s) 343
LguI GCTCTTC 1 cut(s) 3549
LmnI GCTCC 8 cut(s) 644, 889, 1003, 3385, 4342, 5095, 5638, 5844
MbiI CCGCTC 1 cut(s) 639
MfeI CAATTG 2 cut(s) 2271, 3658
MlsI TGGCCA 1 cut(s) 4232
MluI ACGCGT 1 cut(s) 838
MluNI TGGCCA 1 cut(s) 4232
Mly113I GGCGCC 3 cut(s) 259, 277, 2058
MmeI TCCRAC 6 cut(s) 27, 612, 4237, 4260, 4609, 5958
Mox20I TGGCCA 1 cut(s) 4232
Mph1103I ATGCAT 4 cut(s) 1750, 3022, 5813, 6024
MroI TCCGGA 1 cut(s) 759
MroXI GAANNNNTTC 5 cut(s) 767, 2523, 3802, 4350, 5931
MscI TGGCCA 1 cut(s) 4232
MslI CAYNNNNRTG 8 cut(s) 1449, 2372, 2479, 2547, 3429, 4410, 5432, 6006
Msp20I TGGCCA 1 cut(s) 4232
MspA1I CMGCKG 3 cut(s) 2078, 3278, 3550
MspCI CTTAAG 1 cut(s) 3029
MunI CAATTG 2 cut(s) 2271, 3658
Mva1269I GAATGC 1 cut(s) 4557
MvaI CCWGG 7 cut(s) 297, 1582, 3216, 3383, 4310, 5403, 5566
MvnI CGCG 4 cut(s) 663, 840, 1076, 6105
NarI GGCGCC 3 cut(s) 259, 277, 2058
NciI CCSGG 3 cut(s) 219, 5018, 5019
NcoI CCATGG 3 cut(s) 489, 4163, 5825
NdeI CATATG 3 cut(s) 3016, 4342, 5713
NmeAIII GCCGAG 2 cut(s) 745, 1032
NmuCI GTSAC 9 cut(s) 16, 52, 1024, 1505, 2341, 3616, 4047, 5099, 5229
NruI TCGCGA 1 cut(s) 6105
NsiI ATGCAT 4 cut(s) 1750, 3022, 5813, 6024
NspI RCATGY 4 cut(s) 473, 1061, 1448, 5815
NspV TTCGAA 3 cut(s) 8, 1756, 2953
OliI CACNNNNGTG 1 cut(s) 2372
PaeI GCATGC 1 cut(s) 5815
PagI TCATGA 3 cut(s) 2428, 3229, 4102
PaqCI CACCTGC 1 cut(s) 3379
PauI GCGCGC 1 cut(s) 1074
PciI ACATGT 1 cut(s) 1444
PciSI GCTCTTC 1 cut(s) 3549
PcsI WCGNNNNNNNCGW 5 cut(s) 687, 840, 1965, 3094, 3478
PctI GAATGC 1 cut(s) 4557
PdmI GAANNNNTTC 5 cut(s) 767, 2523, 3802, 4350, 5931
PflFI GACNNNGTC 1 cut(s) 794
PfoI TCCNGGA 3 cut(s) 217, 295, 5564
PinAI ACCGGT 1 cut(s) 4265
Ple19I CGATCG 1 cut(s) 516
PluTI GGCGCC 3 cut(s) 262, 280, 2061
PmaCI CACGTG 1 cut(s) 3089
PmlI CACGTG 1 cut(s) 3089
Ppu21I YACGTR 1 cut(s) 3089
PpuMI RGGWCCY 1 cut(s) 5790
PscI ACATGT 1 cut(s) 1444
PshAI GACNNNNGTC 1 cut(s) 3660
PshBI ATTAAT 3 cut(s) 1550, 4322, 4704
PsiI TTATAA 1 cut(s) 3408
Psp124BI GAGCTC 1 cut(s) 3297
Psp5II RGGWCCY 1 cut(s) 5790
Psp6I CCWGG 7 cut(s) 295, 1580, 3214, 3381, 4308, 5401, 5564
PspCI CACGTG 1 cut(s) 3089
PspFI CCCAGC 1 cut(s) 3913
PspGI CCWGG 7 cut(s) 295, 1580, 3214, 3381, 4308, 5401, 5564
PspOMI GGGCCC 1 cut(s) 6041
PspPPI RGGWCCY 1 cut(s) 5790
PstI CTGCAG 2 cut(s) 241, 4981
PstNI CAGNNNCTG 6 cut(s) 245, 2258, 2510, 3578, 3905, 5534
PsyI GACNNNGTC 1 cut(s) 794
PteI GCGCGC 1 cut(s) 1074
PvuI CGATCG 1 cut(s) 516
PvuII CAGCTG 3 cut(s) 2078, 3278, 3550
RruI TCGCGA 1 cut(s) 6105
RseI CAYNNNNRTG 8 cut(s) 1449, 2372, 2479, 2547, 3429, 4410, 5432, 6006
SacI GAGCTC 1 cut(s) 3297
SalI GTCGAC 1 cut(s) 788
SapI GCTCTTC 1 cut(s) 3549
ScaI AGTACT 2 cut(s) 5200, 5516
SfcI CTRYAG 8 cut(s) 237, 1279, 4488, 4977, 5055, 5067, 5746, 5965
SfoI GGCGCC 3 cut(s) 260, 278, 2059
SfuI TTCGAA 3 cut(s) 8, 1756, 2953
SinI GGWCC 4 cut(s) 567, 1994, 3187, 5790
SmaI CCCGGG 1 cut(s) 5019
SmiMI CAYNNNNRTG 8 cut(s) 1449, 2372, 2479, 2547, 3429, 4410, 5432, 6006
SmlI CTYRAG 4 cut(s) 2019, 3029, 4202, 5286
SmoI CTYRAG 4 cut(s) 2019, 3029, 4202, 5286
SpeI ACTAGT 1 cut(s) 337
SphI GCATGC 1 cut(s) 5815
SspDI GGCGCC 3 cut(s) 258, 276, 2057
SspI AATATT 1 cut(s) 1315
SstI GAGCTC 1 cut(s) 3297
StyI CCWWGG 8 cut(s) 197, 489, 2114, 3726, 4163, 5234, 5825, 6067
TaqII GACCGA 1 cut(s) 2292
TatI WGTACW 8 cut(s) 1044, 1441, 2512, 4451, 5135, 5198, 5514, 6131
TauI GCSGC 3 cut(s) 282, 1562, 3418
TseFI GTSAC 9 cut(s) 16, 52, 1024, 1505, 2341, 3616, 4047, 5099, 5229
Tsp45I GTSAC 9 cut(s) 16, 52, 1024, 1505, 2341, 3616, 4047, 5099, 5229
TspGWI ACGGA 5 cut(s) 386, 388, 631, 784, 2255
TspMI CCCGGG 1 cut(s) 5017
Tth111I GACNNNGTC 1 cut(s) 794
Vha464I CTTAAG 1 cut(s) 3029
VneI GTGCAC 2 cut(s) 2248, 2550
VpaK11BI GGWCC 4 cut(s) 567, 1994, 3187, 5790
VspI ATTAAT 3 cut(s) 1550, 4322, 4704
XagI CCTNNNNNAGG 3 cut(s) 3386, 4205, 5238
XbaI TCTAGA 3 cut(s) 1652, 2746, 3664
XceI RCATGY 4 cut(s) 473, 1061, 1448, 5815
XcmI CCANNNNNNNNNTGG 1 cut(s) 2978
XmaI CCCGGG 1 cut(s) 5017
XmaJI CCTAGG 1 cut(s) 197
XmiI GTMKAC 5 cut(s) 138, 789, 3475, 4461, 5862
XmnI GAANNNNTTC 5 cut(s) 767, 2523, 3802, 4350, 5931
ZraI GACGTC 2 cut(s) 99, 3479
ZrmI AGTACT 2 cut(s) 5200, 5516
Zsp2I ATGCAT 4 cut(s) 1750, 3022, 5813, 6024
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.