Rmu_sc0002518.1_g000002

NB-ARC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002518.1
OG0005191
Physical Location & Seq
Forward (+)
5002 .. 8823
3822 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3420 bp
atggccgaaatagcaactcacgctgtggggattgccatcactaaattgtctcctcttgtcaccacggagtttcttgcattcactaatgtcaaggatgacctggaatccctcagaagcactctaacagatgttcgggagttgcttggaattctggaaagaaatcggacgaattcctcaggtttcgaacagatgaacaattggctcaagaagcttcaagtagcagcttatgatgcaggggatttaatcgcctcctgggcagtagaatatcaccaatggaagacgaagaagcaggtacgaaaattctctcttccgtttagtgcttcaaaatttttctttcagcatcaagaatcatgtgacttgagagaaattacagcgagaataaataagattttacaagatggacaagtctatagaacaatcattggagtgcctaggagtgaaagtttttcacggcaaactggctctctttctagcaatataattgagggcaggggagatgatgtagagaacataataaaactgctgataccagatgaaggagccagtgatgagggcagtattgcttttattccaattgtggggatggggggcttgggtaaaacaactctcgctcaactcgtatataagaatggcagggttcataaacattttaaaagaaaattctgggtttgtgtcacagaaacttatgacgagactagaattctcaaacgaatgttggagtctaatgagattacttttgatgccaatatcacattcgatgtactgcaagaccgagttcgtgaactcgtggctgaaaaacgatttttacttgttctagatgatttatggaacaataattatatggatttggagccactagaaaatttactgaaacaaggggtcagtggaagtaaggtcttggttactagtcggaacaatgaagttcggaagattacgggtgcaaaatcctcctatagcttgcgacatttcaatgataaagaatcactgtcgttattcgaaaaaattgcctttgaaggaaggagtccgccagatgagttgaaggaatatgcaaagcaaattgtaaacaagtgcaacggtttacctctggcagtaaaacaaatggggggtctgctacgtggaatcactgatgcaagtgagtggaaatatgtcgctagaagtgaaatatgggaactagaaaaagataaagtactgcctgctcttcgattgagttataatcatctgtcttcagctgtgaagcaatgttttgcgacagaagacataggaagcagatacttcaaggagctgtcagacaggttcttctttgagatctcggcggaagacaagagcaagtgtagaatgcatgatcttatccacgacttggcgctattaatttcctctccctttttctgccaagtggaagatactgtgccaaagagtttagacgaagtatctcgccatgtatcactgctttcgaatgaggttgagcagcccctttcagaaataataaaaaaatcaaagaagctgcgcacacttttattgccagtgaaacacttcaaagcctttgggaaagctgaacgtgcaatattccattcacttaagtacatgcgcacgctagatttgagttcaagcacacttcaagaactgtcaggctcaatcgagaatttgaagcttttgcactaccttgatttatcaaaaactgaaataaaaaagcttcccgattcgatatgcaacctctgccatttggagacgttgaaactttcagattgcccttggcttttcactttgcccaggaagctcaaaactcttgtcaacctgagacatctggagctagatgaaatgttctggtacaaggtatcaacgcttccaaaaagcatggggtgtctaaccagtctacacaatcttcacaaatttcaggttggtcttcttctctctttttctttgttcctgttgttcttctctacacttcatctcccatgtaacaaaatatacacatttcaggttggctgcaatacgggatacaaacttcaagaattgaagaacatggagtaccttacagggttcctgcacatctcaaacctggaaaatgcagtggatgcaggggaggccaacttgaaagaaaaagaaatgattgataaagtggtatatgaatggagcagcagcaatctgaatttgcaagatgaagacgccaagcaggtactcgaagacctgcagcctcacccgagggttcaagagatccaaatctgtcactacagaagccgtgagtttccaatttggatcagatacggaaagtatgagaacttaacttctatatatttgaatcattgcacaaggatcaaaatcctctcacttggcgagttacccaacctcagagaactccgtctcaagaacatggcagaaatgatggaatggaaggaagagaacgacgtgttcctcttgagtctaaagattagtggctgcccaaagctgacaaaactgtccggctactttcctgtattacatagtatgaagatcaagaattgtgaatcattggatacccttccagttggcccagtggaattcataagacttgctggcaatcctgtgctgaagcactggactgaagtcaatgtactaacaaggagagttgtcgatggtcgcctggtcacaagtcatggcatgacaagtcattatctgttggaagcaaacattatcaactgccctgaactgcagaggttgccaagagaactccatccacagaaactggaaataagcggctgcaagaacttgcgcaccctaccagatgcagaccatgcagaatggcttggggttttagcattggatgcatgccatgatgaaacattagttggtatgatccccagttctgggagtttatattccttggtgatttctaacatcccaaatctcatttgtcttccaaaatggcccagtctccccagcctccaagcattgtatatccgtgattgcacagaccttgaatatctattcaaccaagagaatgggaacgggttgttcgatggctactactctctaaagcacctctccatccgtaactgtcctaaacttctgacactgcctgctgaaggtctcccagccagtcttcaagttttgagtattggccaatgtgaaagtctcagttcgtttggccccagggaagtactcgcaaaactcacatcccttggtgatctctaccttgaagattgccctgcactccagtccttgccagaggaaggcctgtcagcgtccctccaacatctgtccatccaaggatgcccctcgctggtaagaagctgtgcaaaagatggcagcgattggccaaagatcaaggacatccccgatctggaaatagagatgccatctactgaaaccacttcagcaccatggtacaatccttttaggaaccgcagaggaaacaagtccaaaggaaagaaggcttcggaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1139

Amino Acids

129.52

Weight (kDa)

6.9

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 100 3.6e-13 Rx N-terminal domain
NB-ARC PF00931 168 - 340 1.3e-30 NB-ARC domain
WHD_DRP PF23559 417 - 454 1e-09 Disease resistance protein Winged helix domain
LRR_14 PF23598 515 - 630 1.4e-13 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 668 - 790 5.4e-28 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1212
AasI GACNNNNNNGTC 1 cut(s) 2452
Acc16I TGCGCA 3 cut(s) 1505, 1586, 2747
Acc36I ACCTGC 3 cut(s) 280, 2161, 2193
AccB7I CCANNNNNTGG 1 cut(s) 1357
AccI GTMKAC 1 cut(s) 1870
AciI CCGC 4 cut(s) 1025, 1313, 2730, 3379
AclWI GGATC 4 cut(s) 2206, 2261, 2318, 2824
AcoI YGGCCR 3 cut(s) 3, 3094, 3290
AcuI CTGAAG 5 cut(s) 1209, 2585, 2598, 3078, 3333
AcyI GRCGYC 1 cut(s) 2163
AdeI CACNNNGTG 1 cut(s) 25
AfiI CCNNNNNNNGG 9 cut(s) 536, 578, 1357, 2840, 2841, 3059, 3206, 3256, 3316
AflII CTTAAG 1 cut(s) 1574
AflIII ACRYGT 1 cut(s) 2403
AhlI ACTAGT 1 cut(s) 905
AjiI CACGTC 1 cut(s) 2404
AjnI CCWGG 6 cut(s) 99, 251, 1766, 2055, 2616, 3125
AjuI GAANNNNNNNTTGG 4 cut(s) 737, 769, 2805, 2837
Alw26I GTCTC 8 cut(s) 54, 686, 1718, 1789, 2363, 2912, 3068, 3113
AlwI GGATC 4 cut(s) 2206, 2261, 2318, 2824
AlwNI CAGNNNCTG 1 cut(s) 2719
Ama87I CYCGRG 1 cut(s) 2197
AoxI GGCC 8 cut(s) 3, 2082, 2524, 2900, 3094, 3121, 3208, 3290
ArsI GACNNNNNNTTYG 4 cut(s) 1090, 1122, 1770, 1802
AseI ATTAAT 1 cut(s) 1367
Asp700I GAANNNNTTC 1 cut(s) 1529
AspA2I CCTAGG 1 cut(s) 431
AspLEI GCGC 4 cut(s) 1363, 1506, 1587, 2748
AspS9I GGNCC 3 cut(s) 2525, 2901, 3122
AsuHPI GGTGA 5 cut(s) 52, 260, 2186, 2872, 3170
AsuII TTCGAA 3 cut(s) 183, 996, 1451
AvaI CYCGRG 1 cut(s) 2197
AvrII CCTAGG 1 cut(s) 431
AxyI CCTNAGG 1 cut(s) 175
BalI TGGCCA 2 cut(s) 3096, 3292
BarI GAAGNNNNNNTAC 4 cut(s) 1824, 1856, 2571, 2603
BauI CACGAG 1 cut(s) 785
BbsI GAAGAC 9 cut(s) 284, 1215, 1260, 1323, 1892, 2166, 2187, 2882, 3068
BceAI ACGGC 2 cut(s) 467, 2220
BcgI CGANNNNNNTGC 2 cut(s) 986, 1020
BciT130I CCWGG 6 cut(s) 101, 253, 1768, 2057, 2618, 3127
BciVI GTATCC 2 cut(s) 1988, 2503
BcoDI GTCTC 8 cut(s) 54, 686, 1718, 1789, 2363, 2912, 3068, 3113
BcuI ACTAGT 1 cut(s) 905
BfmI CTRYAG 5 cut(s) 409, 952, 2186, 2227, 2684
BfoI RGCGCY 1 cut(s) 1364
BfrI CTTAAG 1 cut(s) 1574
BfuAI ACCTGC 3 cut(s) 280, 2161, 2193
BfuI GTATCC 2 cut(s) 1988, 2503
BglI GCCNNNNNGGC 1 cut(s) 254
BglII AGATCT 1 cut(s) 1305
BlnI CCTAGG 1 cut(s) 431
BmcAI AGTACT 2 cut(s) 1188, 3135
Bme1390I CCNGG 6 cut(s) 101, 253, 1768, 2057, 2618, 3127
BmeT110I CYCGRG 1 cut(s) 2197
BmgBI CACGTC 1 cut(s) 2404
BmgT120I GGNCC 3 cut(s) 2525, 2901, 3122
BmiI GGNNCC 5 cut(s) 541, 852, 2039, 3124, 3377
BmrFI CCNGG 6 cut(s) 101, 253, 1768, 2057, 2618, 3127
BmrI ACTGGG 3 cut(s) 2522, 2829, 2898
BmsI GCATC 9 cut(s) 220, 349, 730, 1117, 2062, 2749, 2788, 3236, 3318
BmuI ACTGGG 3 cut(s) 2522, 2829, 2898
BoxI GACNNNNGTC 1 cut(s) 2579
BpiI GAAGAC 9 cut(s) 284, 1215, 1260, 1323, 1892, 2166, 2187, 2882, 3068
BpmI CTGGAG 2 cut(s) 1823, 3173
Bpu14I TTCGAA 3 cut(s) 183, 996, 1451
BpuEI CTTGAG 4 cut(s) 188, 379, 2345, 2434
BsaAI YACGTR 1 cut(s) 1115
BsaBI GATNNNNATC 3 cut(s) 35, 2216, 2315
BsaHI GRCGYC 1 cut(s) 2163
BsaI GGTCTC 1 cut(s) 3068
BsaXI ACNNNNNCTCC 6 cut(s) 427, 457, 486, 516, 1932, 1962
Bsc4I CCNNNNNNNGG 9 cut(s) 536, 578, 1357, 2840, 2841, 3059, 3206, 3256, 3316
Bse21I CCTNAGG 1 cut(s) 175
Bse3DI GCAATG 2 cut(s) 1244, 2299
Bse8I GATNNNNATC 3 cut(s) 35, 2216, 2315
BseBI CCWGG 6 cut(s) 101, 253, 1768, 2057, 2618, 3127
BseJI GATNNNNATC 3 cut(s) 35, 2216, 2315
BseLI CCNNNNNNNGG 9 cut(s) 536, 578, 1357, 2840, 2841, 3059, 3206, 3256, 3316
BseMI GCAATG 2 cut(s) 1244, 2299
BseMII CTCAG 5 cut(s) 124, 189, 1784, 2359, 3124
BseRI GAGGAG 1 cut(s) 42
BseYI CCCAGC 2 cut(s) 2912, 3067
BsgI GTGCAG 2 cut(s) 2027, 3168
BshFI GGCC 8 cut(s) 5, 2084, 2526, 2902, 3096, 3123, 3210, 3292
BsiHKCI CYCGRG 1 cut(s) 2197
BsiSI CCGG 1 cut(s) 2457
BslFI GGGAC 1 cut(s) 3205
BslI CCNNNNNNNGG 9 cut(s) 536, 578, 1357, 2840, 2841, 3059, 3206, 3256, 3316
BsmAI GTCTC 8 cut(s) 54, 686, 1718, 1789, 2363, 2912, 3068, 3113
BsmBI CGTCTC 2 cut(s) 1718, 2363
BsmFI GGGAC 1 cut(s) 3205
BsmI GAATGC 2 cut(s) 77, 1341
BsnI GGCC 8 cut(s) 5, 2084, 2526, 2902, 3096, 3123, 3210, 3292
Bso31I GGTCTC 1 cut(s) 3068
BsoBI CYCGRG 1 cut(s) 2197
Bsp119I TTCGAA 3 cut(s) 183, 996, 1451
Bsp19I CCATGG 1 cut(s) 3356
BspACI CCGC 4 cut(s) 1025, 1313, 2730, 3379
BspANI GGCC 8 cut(s) 5, 2084, 2526, 2902, 3096, 3123, 3210, 3292
BspCNI CTCAG 5 cut(s) 123, 188, 1785, 2358, 3123
BspLI GGNNCC 5 cut(s) 541, 852, 2039, 3124, 3377
BspMAI CTGCAG 2 cut(s) 2190, 2688
BspMI ACCTGC 3 cut(s) 280, 2161, 2193
BspPI GGATC 4 cut(s) 2206, 2261, 2318, 2824
BspQI GCTCTTC 1 cut(s) 1203
BspT104I TTCGAA 3 cut(s) 183, 996, 1451
BspTI CTTAAG 1 cut(s) 1574
BspTNI GGTCTC 1 cut(s) 3068
BsrDI GCAATG 2 cut(s) 1244, 2299
BssNI GRCGYC 1 cut(s) 2163
BssSI CACGAG 1 cut(s) 785
BssT1I CCWWGG 6 cut(s) 431, 1748, 2856, 3154, 3241, 3356
Bst2BI CACGAG 1 cut(s) 785
Bst2UI CCWGG 6 cut(s) 101, 253, 1768, 2057, 2618, 3127
Bst4CI ACNGT 6 cut(s) 987, 1076, 1405, 1623, 2454, 3032
Bst6I CTCTTC 3 cut(s) 312, 1203, 2388
BstACI GRCGYC 1 cut(s) 2163
BstAFI CTTAAG 1 cut(s) 1574
BstAPI GCANNNNNTGC 5 cut(s) 1713, 2072, 2692, 2768, 2798
BstBAI YACGTR 1 cut(s) 1115
BstBI TTCGAA 3 cut(s) 183, 996, 1451
BstC8I GCNNGC 6 cut(s) 959, 1194, 1589, 2551, 2803, 3054
BstDEI CTNAG 5 cut(s) 110, 175, 1793, 2345, 3110
BstDSI CCRYGG 2 cut(s) 63, 3356
BstENI CCTNNNNNAGG 1 cut(s) 3057
BstH2I RGCGCY 1 cut(s) 1364
BstHHI GCGC 4 cut(s) 1363, 1506, 1587, 2748
BstMAI GTCTC 8 cut(s) 54, 686, 1718, 1789, 2363, 2912, 3068, 3113
BstNI CCWGG 6 cut(s) 101, 253, 1768, 2057, 2618, 3127
BstNSI RCATGY 2 cut(s) 1585, 2805
BstPAI GACNNNNGTC 1 cut(s) 2579
BstSCI CCNGG 6 cut(s) 99, 251, 1766, 2055, 2616, 3125
BstSFI CTRYAG 5 cut(s) 409, 952, 2186, 2227, 2684
BstV2I GAAGAC 9 cut(s) 284, 1215, 1260, 1323, 1892, 2166, 2187, 2882, 3068
BstX2I RGATCY 2 cut(s) 1305, 2211
BstXI CCANNNNNNTGG 1 cut(s) 2975
BstYI RGATCY 2 cut(s) 1305, 2211
Bsu36I CCTNAGG 1 cut(s) 175
BsuI GTATCC 2 cut(s) 1988, 2503
BsuRI GGCC 8 cut(s) 5, 2084, 2526, 2902, 3096, 3123, 3210, 3292
BtgI CCRYGG 2 cut(s) 63, 3356
BtrI CACGTC 1 cut(s) 2404
BtsI GCAGTG 3 cut(s) 1442, 2073, 3047
BveI ACCTGC 3 cut(s) 280, 2161, 2193
Cac8I GCNNGC 6 cut(s) 959, 1194, 1589, 2551, 2803, 3054
CaiI CAGNNNCTG 1 cut(s) 2719
CfoI GCGC 4 cut(s) 1363, 1506, 1587, 2748
Cfr13I GGNCC 3 cut(s) 2525, 2901, 3122
CseI GACGC 2 cut(s) 2171, 3207
DdeI CTNAG 5 cut(s) 110, 175, 1793, 2345, 3110
DraI TTTAAA 1 cut(s) 652
DraIII CACNNNGTG 1 cut(s) 25
DrdI GACNNNNNNGTC 1 cut(s) 2452
DseDI GACNNNNNNGTC 1 cut(s) 2452
EaeI YGGCCR 3 cut(s) 3, 3094, 3290
Eam1104I CTCTTC 3 cut(s) 312, 1203, 2388
EarI CTCTTC 3 cut(s) 312, 1203, 2388
EciI GGCGGA 2 cut(s) 1014, 1328
Eco130I CCWWGG 6 cut(s) 431, 1748, 2856, 3154, 3241, 3356
Eco147I AGGCCT 1 cut(s) 3210
Eco31I GGTCTC 1 cut(s) 3068
Eco57I CTGAAG 5 cut(s) 1209, 2585, 2598, 3078, 3333
Eco81I CCTNAGG 1 cut(s) 175
Eco88I CYCGRG 1 cut(s) 2197
EcoNI CCTNNNNNAGG 1 cut(s) 3057
EcoRI GAATTC 4 cut(s) 147, 169, 699, 2534
EcoRII CCWGG 6 cut(s) 99, 251, 1766, 2055, 2616, 3125
EcoT14I CCWWGG 6 cut(s) 431, 1748, 2856, 3154, 3241, 3356
EcoT22I ATGCAT 2 cut(s) 1341, 2803
ErhI CCWWGG 6 cut(s) 431, 1748, 2856, 3154, 3241, 3356
Esp3I CGTCTC 2 cut(s) 1718, 2363
FaqI GGGAC 1 cut(s) 3205
FblI GTMKAC 1 cut(s) 1870
FspAI RTGCGCAY 1 cut(s) 1586
FspI TGCGCA 3 cut(s) 1505, 1586, 2747
GlaI GCGC 4 cut(s) 1362, 1505, 1586, 2747
GsaI CCCAGC 2 cut(s) 2916, 3071
GsuI CTGGAG 2 cut(s) 1823, 3173
HaeII RGCGCY 1 cut(s) 1364
HaeIII GGCC 8 cut(s) 5, 2084, 2526, 2902, 3096, 3123, 3210, 3292
HapII CCGG 1 cut(s) 2457
HgaI GACGC 2 cut(s) 2171, 3207
HhaI GCGC 4 cut(s) 1363, 1506, 1587, 2748
Hin1I GRCGYC 1 cut(s) 2163
Hin6I GCGC 4 cut(s) 1361, 1504, 1585, 2746
HinP1I GCGC 4 cut(s) 1361, 1504, 1585, 2746
HincII GTYRAC 1 cut(s) 1789
HindII GTYRAC 1 cut(s) 1789
HindIII AAGCTT 3 cut(s) 209, 1646, 1688
HpaII CCGG 1 cut(s) 2457
HphI GGTGA 5 cut(s) 52, 260, 2186, 2872, 3170
Hpy166II GTNNAC 5 cut(s) 782, 1063, 1079, 1789, 1871
Hpy8I GTNNAC 5 cut(s) 782, 1063, 1079, 1789, 1871
Hpy99I CGWCG 1 cut(s) 2405
HpyAV CCTTC 9 cut(s) 530, 1007, 1011, 1033, 2383, 2525, 3053, 3200, 3400
HpyCH4III ACNGT 6 cut(s) 987, 1076, 1405, 1623, 2454, 3032
HpyCH4IV ACGT 4 cut(s) 1114, 1555, 1727, 2403
HpyF3I CTNAG 5 cut(s) 110, 175, 1793, 2345, 3110
HpySE526I ACGT 4 cut(s) 1114, 1555, 1727, 2403
Hsp92I GRCGYC 1 cut(s) 2163
HspAI GCGC 4 cut(s) 1361, 1504, 1585, 2746
LguI GCTCTTC 1 cut(s) 1203
LmnI GCTCC 5 cut(s) 539, 850, 1279, 1804, 2130
LweI GCATC 9 cut(s) 220, 349, 730, 1117, 2062, 2749, 2788, 3236, 3318
MaeII ACGT 4 cut(s) 1114, 1555, 1727, 2403
MaeIII GTNAC 9 cut(s) 58, 353, 673, 901, 1955, 2222, 2334, 2620, 3026
MfeI CAATTG 2 cut(s) 196, 573
MflI RGATCY 2 cut(s) 1305, 2211
MlsI TGGCCA 2 cut(s) 3096, 3292
MluNI TGGCCA 2 cut(s) 3096, 3292
MlyI GAGTC 3 cut(s) 728, 1030, 2425
MmeI TCCRAC 4 cut(s) 696, 890, 2634, 3250
Mox20I TGGCCA 2 cut(s) 3096, 3292
Mph1103I ATGCAT 2 cut(s) 1341, 2803
MroXI GAANNNNTTC 1 cut(s) 1529
MscI TGGCCA 2 cut(s) 3096, 3292
MseI TTAA 5 cut(s) 242, 651, 1367, 1575, 2279
MslI CAYNNNNRTG 2 cut(s) 425, 969
Msp20I TGGCCA 2 cut(s) 3096, 3292
MspA1I CMGCKG 1 cut(s) 1229
MspCI CTTAAG 1 cut(s) 1574
MspI CCGG 1 cut(s) 2457
MspR9I CCNGG 6 cut(s) 101, 253, 1768, 2057, 2618, 3127
MunI CAATTG 2 cut(s) 196, 573
Mva1269I GAATGC 2 cut(s) 77, 1341
MvaI CCWGG 6 cut(s) 101, 253, 1768, 2057, 2618, 3127
NcoI CCATGG 1 cut(s) 3356
NlaIV GGNNCC 5 cut(s) 541, 852, 2039, 3124, 3377
NmeAIII GCCGAG 1 cut(s) 1289
NmuCI GTSAC 5 cut(s) 58, 353, 673, 2222, 2620
NsbI TGCGCA 3 cut(s) 1505, 1586, 2747
NsiI ATGCAT 2 cut(s) 1341, 2803
NspI RCATGY 2 cut(s) 1585, 2805
NspV TTCGAA 3 cut(s) 183, 996, 1451
PaeI GCATGC 1 cut(s) 2805
PasI CCCWGGG 1 cut(s) 3126
PceI AGGCCT 1 cut(s) 3210
PciSI GCTCTTC 1 cut(s) 1203
PcsI WCGNNNNNNNCGW 2 cut(s) 615, 2988
PctI GAATGC 2 cut(s) 77, 1341
PdmI GAANNNNTTC 1 cut(s) 1529
PfeI GAWTC 7 cut(s) 104, 347, 980, 1119, 1697, 2296, 2501
PflMI CCANNNNNTGG 1 cut(s) 1357
PleI GAGTC 3 cut(s) 727, 1029, 2424
PpsI GAGTC 3 cut(s) 727, 1029, 2424
Ppu21I YACGTR 1 cut(s) 1115
PshAI GACNNNNGTC 1 cut(s) 2579
PshBI ATTAAT 1 cut(s) 1367
PsiI TTATAA 1 cut(s) 1212
Psp6I CCWGG 6 cut(s) 99, 251, 1766, 2055, 2616, 3125
PspFI CCCAGC 2 cut(s) 2912, 3067
PspGI CCWGG 6 cut(s) 99, 251, 1766, 2055, 2616, 3125
PspN4I GGNNCC 5 cut(s) 541, 852, 2039, 3124, 3377
PspPI GGNCC 3 cut(s) 2525, 2901, 3122
PsrI GAACNNNNNNTAC 2 cut(s) 2009, 2041
PstI CTGCAG 2 cut(s) 2190, 2688
PstNI CAGNNNCTG 1 cut(s) 2719
PsuI RGATCY 2 cut(s) 1305, 2211
PvuII CAGCTG 1 cut(s) 1229
RseI CAYNNNNRTG 2 cut(s) 425, 969
SapI GCTCTTC 1 cut(s) 1203
SaqAI TTAA 5 cut(s) 242, 651, 1367, 1575, 2279
Sau96I GGNCC 3 cut(s) 2525, 2901, 3122
ScaI AGTACT 2 cut(s) 1188, 3135
SchI GAGTC 3 cut(s) 728, 1030, 2425
ScrFI CCNGG 6 cut(s) 101, 253, 1768, 2057, 2618, 3127
SfaNI GCATC 9 cut(s) 220, 349, 730, 1117, 2062, 2749, 2788, 3236, 3318
SfcI CTRYAG 5 cut(s) 409, 952, 2186, 2227, 2684
SfuI TTCGAA 3 cut(s) 183, 996, 1451
SmiMI CAYNNNNRTG 2 cut(s) 425, 969
SmlI CTYRAG 5 cut(s) 203, 358, 1574, 2360, 2413
SmoI CTYRAG 5 cut(s) 203, 358, 1574, 2360, 2413
SpeI ACTAGT 1 cut(s) 905
SphI GCATGC 1 cut(s) 2805
SseBI AGGCCT 1 cut(s) 3210
SsiI CCGC 4 cut(s) 1025, 1313, 2730, 3379
SspI AATATT 1 cut(s) 1563
StuI AGGCCT 1 cut(s) 3210
StyD4I CCNGG 6 cut(s) 99, 251, 1766, 2055, 2616, 3125
StyI CCWWGG 6 cut(s) 431, 1748, 2856, 3154, 3241, 3356
TaaI ACNGT 6 cut(s) 987, 1076, 1405, 1623, 2454, 3032
TaiI ACGT 4 cut(s) 1117, 1558, 1730, 2406
TaqII GACCGA 1 cut(s) 786
TatI WGTACW 5 cut(s) 760, 1186, 1578, 2587, 3133
TauI GCSGC 1 cut(s) 2733
TfiI GAWTC 7 cut(s) 104, 347, 980, 1119, 1697, 2296, 2501
Tru1I TTAA 5 cut(s) 242, 651, 1367, 1575, 2279
Tru9I TTAA 5 cut(s) 242, 651, 1367, 1575, 2279
TseFI GTSAC 5 cut(s) 58, 353, 673, 2222, 2620
Tsp45I GTSAC 5 cut(s) 58, 353, 673, 2222, 2620
TspGWI ACGGA 6 cut(s) 80, 300, 2277, 2345, 2924, 3014
Van91I CCANNNNNTGG 1 cut(s) 1357
Vha464I CTTAAG 1 cut(s) 1574
VspI ATTAAT 1 cut(s) 1367
XagI CCTNNNNNAGG 1 cut(s) 3057
XbaI TCTAGA 1 cut(s) 814
XceI RCATGY 2 cut(s) 1585, 2805
XmaJI CCTAGG 1 cut(s) 431
XmiI GTMKAC 1 cut(s) 1870
XmnI GAANNNNTTC 1 cut(s) 1529
ZrmI AGTACT 2 cut(s) 1188, 3135
Zsp2I ATGCAT 2 cut(s) 1341, 2803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.