Rmu_sc0002652.1_g000022

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002652.1
OG0006832
Physical Location & Seq
Reverse (-)
59735 .. 66404
6670 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4170 bp
atggacacgaataattggaggcctcctcaaggtggagagcctgcaatggatgcaggagattggaggagccaattacagcaggattcgcggcagagaatcgtcaataagataatggatacattgaagaggcatcttcccttctccggtcaagagggattgcacgaactcaagaaaattgctgttaggtttgaggaaaagatttatactgctgctacaagccagtcggattatctacggaaaatttctctgaaaatgctcactatggagaccaagtctcagaacacaattggcacttctttaccaagcaactctgctggtaatagtaacaggccccctgatccaggatctttcatgcaaccccaagttcccaatcaaggacagtcactccctatgccagtgccagccaatcaatctcaaccacgccaacaactcttatcacagaacattcagaataacattccgcccaattctgctggcttatcatctgcattatctcctgcatctgggctcacccagactcctgtaccgagtattgttggaaatgcaaacttgcaaagtatgtcggggacatcacagaattcattggggaactccatcggccagggggtaacttcaaatatgtttgctaattcccaaaggcaaatgccaggacgacaacaggtagttccgcagcagcaacagcagcaaacccagaattcacagcagtatatgtaccagcaacagttacaacaacagctcatgaagccaaaatttcagcagcagggaaacatccaacttgtgcaaaatcatatgcaacagcaacaacagcagcagcagaacttattgcaatcacctcagttgcaatcttctcagcaatctgttatgcaacctcctgcagtgcagtcgtctctctctagtcttcagcagaatcaacaatctgctatgcaacactcttcacaagccatgcttcagcagcattctcaatctgttctgcggcagcaacagcagcctcaacagtcttctgttgttcatcagcagcaaacaccaatcccacagcagtcaatactgcccccacagcagcagcagcaacagccacagcttatggtgcaacagccaaattcaacaaacttgccgcagaatcagcttattggacaacaaaacaatgtaggggacatgcagcaacagcaacagcaacagcaacagcaacagcaacagcaacagcaacagcaaaggttactaagccagcagaggcttattggacaacaaaacaatgtaggggacatgcagcaacagcaacagcaacagcaacagcaacagcaacagcaacagcaaaggttactaagccagcagaataatatgttaaacttgcaacagcagcagcagcaacaacaacaccaattaatggctcagcaaaacaacctctcaaatatgcatcagcaacagttgggcactcagagtaatgttactggattacagcaacagcagcaccttggtactcaaactggtaactcaagcatgcaaactaatcagcactctgcacacatgctgcaatccaaggttcagcaacaacatcagagtccagcattaccgattcaaggacatcagtcccaacctcaggtgtcacagcaacaattattatcacagattcaatctcagcctgcacagatacaacaacagatgggtatgcaacagcaatcaaaccagctacaacgagatctgcagcaaaggatccaagcatcaggtcaggtatcaggatccatgcttcaaccaccaaatgtaatggatccgcaaaagcagttatatcaagctcaaagagcccttcctgagacgtcatcaacgtctctagattccacagcccagactggacatacaaatggtgctgattggcaagaggaggtctatcaaaagatcaaagtcatgaaggatatgtactaccccgaactaagtgaaatgtaccaaaaaattgctactaaacttcatcagcacgactcccttccacaacagccaaagtcagatcagcttgagaagctgaagatgtttaagaccatgctggagcgcctgataacagtcttacaaatttccaaaactagcataacacctggtttgaaggagaagttgggcttatacgagaagcagataataaattttattaatacaaatagacccaggaagcctgtttcttctctgccgccagggcagctccccccaccgcatatgcagtccatgcagcagtcacaatcccagattactcaagtgcaatctcatgaaaatcagatgaacccacaattgcaatccctgaacttgcaaggttctgtgccaacaatgcagcagaacaatatgacgggcttgcagcaaaattctatgtcttctttatctggggtttcaacagcacagcaaaacatgatgaatccgttgcagccaagttccaatatggatccaggacaaggaaccgcactgaactcactgcagcaggtccctgtgggctctatccaacagactcctgtcagtgctccccaacaagctaacatgaatgccttatcatcacagaatgggattaacatgctgcagagcaacattaatccccttcagtcaaattctggtatgcttcatagccaacatctgaaacagcaggaacagcaattgtatcagaatcaactcaagcaacaacaatttcaacaacgacagatgcagcagcagcagttgatgcagaagcagctaattcaacaacagcagcagcagcaacagcaacagcaacaacagctacaacagcaagcaaagcagcagctgcctggacagttgcaggcacaccaaatgccacagcttcaccaaatcagtgatgttaatgatgtgaaagtaaggccagggatgggtgttaagtcaggggcctttcagcaacaaatgtctgtaggccagcgtgcttatcctcattcgcaattaaaaccgggatctccatatcctatcacatcaagcaaccaactccttcaggctcaatctcctcaactgtcacagcattcttctccacaagttgaccagccgatgttagtcccaaaagctcaaaatgctagctcaccatttgtcatcccatctccttcaactcccatagcgccatcacctatgccaggagattcagagaaaccttcctcactcgcaaatgctgggaatgttggacatcaacaagcaactgttgtcggagctcaggtccagtcccttgccatcggcacccctgggatatcagcctctcctttgcttgctgaatttatcggtccagatggcactcatgctaatgctttgtcagcaatatctggcaagtcaagtgttacagagcaacctcttgaacgcttgattaaagcggtcaagtcaatatcaccaaaggcattaagtgcatctgtcagtgacataggctcagtagtgagtatgattgataggatagcaggttcagcaccaggtaatggatctagagctgcagttggtgaggatctggttgctatgacaaaatgtcgtctacaggcgagaaacttcatcactcatgatggaacaacaaatgggacaaggaaaatgaggcgatacactagtgccatgcccttaaatgttgtgtcatcaggtggcagcatgaatgatagtttcaagcagttgacctgttcagagacatctgacctggagtcaactgcaacgtctcgtatcaaaaggcctaggattgaggctaatcatgctcttatggaagaaataagagaaataaatcggcggcttatagatacagttgttgatatcagtgatgaagatgttgatccaagtgcggctgctgctgaagggggtgaaggaaccatcgtcaagtgctcttttgatgctgtggctctcagtcccaacttgaaatcgcagtatgcttcagcacaaatgtctccgattcagccattaagactgcttgttccgacaaattatcccaactgctctccaatactgttagacaagtttccggttgaagtcagtaaggagtatgaagatctttctgtgaaggcaaaatcaaggtttagcatctctttacgaagcatttcgcagcccatgtcccttggggagatagcaagggcttgggataattgtgcacgtacagttatttctgagcatgcacagcgaagtggtggaggcagcttcagctcaaagtatgggacatgggagaactgcctgagtgccgcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

1389

Amino Acids

153.77

Weight (kDa)

9.43

Isoelectric Point (pI)

73.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 19 - 97 2.2e-35 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1811
Acc36I ACCTGC 2 cut(s) 2440, 3401
AccB1I GGYRCC 1 cut(s) 3197
AccB7I CCANNNNNTGG 2 cut(s) 1381, 3428
AccI GTMKAC 1 cut(s) 3481
AccII CGCG 1 cut(s) 88
AcoI YGGCCR 1 cut(s) 598
AcuI CTGAAG 8 cut(s) 884, 932, 2030, 2549, 2945, 3804, 3846, 4108
AcyI GRCGYC 1 cut(s) 1808
AfaI GTAC 6 cut(s) 525, 713, 1474, 1910, 1934, 4081
AhdI GACNNNNNGTC 2 cut(s) 3474, 3637
AhlI ACTAGT 1 cut(s) 3548
AjuI GAANNNNNNNTTGG 2 cut(s) 2078, 2110
AloI GAACNNNNNNTCC 2 cut(s) 3397, 3429
Alw21I GWGCWC 4 cut(s) 2491, 3175, 3815, 4078
Alw26I GTCTC 8 cut(s) 260, 279, 891, 1799, 1824, 3617, 3657, 3879
Alw44I GTGCAC 1 cut(s) 4074
AlwNI CAGNNNCTG 1 cut(s) 2764
AoxI GGCC 7 cut(s) 20, 329, 598, 2837, 2862, 2887, 3665
ApaLI GTGCAC 1 cut(s) 4074
AseI ATTAAT 3 cut(s) 1379, 2130, 2556
Asp700I GAANNNNTTC 1 cut(s) 4024
AspA2I CCTAGG 1 cut(s) 3668
AspLEI GCGC 2 cut(s) 2037, 3085
AspS9I GGNCC 5 cut(s) 330, 2452, 2862, 3178, 3242
AsuC2I CCSGG 1 cut(s) 2922
AsuHPI GGTGA 8 cut(s) 502, 822, 2795, 3039, 3081, 3336, 3461, 3803
AsuNHI GCTAGC 1 cut(s) 3041
AvaII GGWCC 3 cut(s) 2452, 3178, 3242
AvrII CCTAGG 1 cut(s) 3668
AxyI CCTNAGG 1 cut(s) 1593
BaeGI GKGCMC 2 cut(s) 1430, 4078
BaeI ACNNNNGTAYC 6 cut(s) 507, 540, 3535, 3568, 3723, 3756
BamHI GGATCC 4 cut(s) 1707, 1733, 1762, 2413
BanI GGYRCC 1 cut(s) 3197
BanII GRGCYC 4 cut(s) 510, 1798, 2465, 3175
BarI GAAGNNNNNNTAC 2 cut(s) 1892, 1924
BbsI GAAGAC 3 cut(s) 890, 990, 2337
Bbv12I GWGCWC 4 cut(s) 2491, 3175, 3815, 4078
BccI CCATC 9 cut(s) 602, 1651, 2839, 3070, 3094, 3200, 3242, 3503, 3809
BcgI CGANNNNNNTGC 2 cut(s) 864, 898
BciVI GTATCC 1 cut(s) 109
BcnI CCSGG 1 cut(s) 2922
BcoDI GTCTC 8 cut(s) 260, 279, 891, 1799, 1824, 3617, 3657, 3879
BcuI ACTAGT 1 cut(s) 3548
BfaI CTAG 7 cut(s) 894, 1823, 2067, 3042, 3435, 3549, 3669
BfmI CTRYAG 7 cut(s) 873, 1697, 2444, 2543, 2883, 3441, 3482
BfoI RGCGCY 2 cut(s) 2038, 3086
BfuAI ACCTGC 2 cut(s) 2440, 3401
BfuI GTATCC 1 cut(s) 109
BglI GCCNNNNNGGC 1 cut(s) 2173
BglII AGATCT 2 cut(s) 1693, 3976
BlnI CCTAGG 1 cut(s) 3668
BlpI GCTNAGC 1 cut(s) 1386
Bme18I GGWCC 3 cut(s) 2452, 3178, 3242
BmeRI GACNNNNNGTC 2 cut(s) 3474, 3637
BmgT120I GGNCC 5 cut(s) 330, 2452, 2862, 3178, 3242
BmtI GCTAGC 1 cut(s) 3045
BoxI GACNNNNGTC 2 cut(s) 1582, 2480
BpiI GAAGAC 3 cut(s) 890, 990, 2337
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpmI CTGGAG 2 cut(s) 2051, 3656
Bpu10I CCTNAGC 1 cut(s) 3174
Bpu1102I GCTNAGC 1 cut(s) 1386
BpuEI CTTGAG 6 cut(s) 12, 152, 1474, 2021, 2214, 2621
BpuMI CCSGG 1 cut(s) 2922
BsaAI YACGTR 1 cut(s) 4079
BsaBI GATNNNNATC 1 cut(s) 3762
BsaHI GRCGYC 1 cut(s) 1808
BsaI GGTCTC 1 cut(s) 260
BsaWI WCCGGW 2 cut(s) 143, 3949
BsaXI ACNNNNNCTCC 8 cut(s) 257, 287, 369, 399, 502, 532, 3162, 3192
Bse1I ACTGG 6 cut(s) 220, 395, 1450, 1486, 1846, 3181
Bse21I CCTNAGG 1 cut(s) 1593
Bse3DI GCAATG 1 cut(s) 51
Bse8I GATNNNNATC 1 cut(s) 3762
BseGI GGATG 4 cut(s) 55, 768, 2850, 3057
BseJI GATNNNNATC 1 cut(s) 3762
BseMI GCAATG 1 cut(s) 51
BseNI ACTGG 6 cut(s) 220, 395, 1450, 1486, 1846, 3181
BseRI GAGGAG 4 cut(s) 15, 79, 1886, 2964
BseSI GKGCMC 2 cut(s) 1430, 4078
BseYI CCCAGC 1 cut(s) 3134
BsgI GTGCAG 3 cut(s) 899, 1500, 1623
Bsh1236I CGCG 1 cut(s) 88
BshFI GGCC 7 cut(s) 22, 331, 600, 2839, 2864, 2889, 3667
BshNI GGYRCC 1 cut(s) 3197
BsiHKAI GWGCWC 4 cut(s) 2491, 3175, 3815, 4078
BsiSI CCGG 3 cut(s) 144, 2921, 3950
BsmAI GTCTC 8 cut(s) 260, 279, 891, 1799, 1824, 3617, 3657, 3879
BsmBI CGTCTC 4 cut(s) 891, 1799, 1824, 3657
BsmI GAATGC 3 cut(s) 955, 2515, 2989
BsnI GGCC 7 cut(s) 22, 331, 600, 2839, 2864, 2889, 3667
Bso31I GGTCTC 1 cut(s) 260
Bsp1286I GDGCHC 8 cut(s) 510, 1430, 1798, 2465, 2491, 3175, 3815, 4078
Bsp1720I GCTNAGC 1 cut(s) 1386
BspANI GGCC 7 cut(s) 22, 331, 600, 2839, 2864, 2889, 3667
BspFNI CGCG 1 cut(s) 88
BspHI TCATGA 4 cut(s) 738, 1896, 2242, 3505
BspMAI CTGCAG 5 cut(s) 877, 1701, 2448, 2547, 3445
BspMI ACCTGC 2 cut(s) 2440, 3401
BspOI GCTAGC 1 cut(s) 3045
BspT107I GGYRCC 1 cut(s) 3197
BspTNI GGTCTC 1 cut(s) 260
BsrDI GCAATG 1 cut(s) 51
BsrI ACTGG 6 cut(s) 220, 395, 1450, 1486, 1846, 3181
BssNI GRCGYC 1 cut(s) 1808
BssT1I CCWWGG 4 cut(s) 1468, 1533, 3668, 4042
Bst6I CTCTTC 2 cut(s) 119, 937
BstACI GRCGYC 1 cut(s) 1808
BstAPI GCANNNNNTGC 5 cut(s) 50, 2203, 2683, 2764, 3359
BstBAI YACGTR 1 cut(s) 4079
BstENI CCTNNNNNAGG 3 cut(s) 27, 339, 3096
BstF5I GGATG 4 cut(s) 55, 768, 2850, 3057
BstFNI CGCG 1 cut(s) 88
BstH2I RGCGCY 2 cut(s) 2038, 3086
BstHHI GCGC 2 cut(s) 2037, 3085
BstMAI GTCTC 8 cut(s) 260, 279, 891, 1799, 1824, 3617, 3657, 3879
BstNSI RCATGY 6 cut(s) 1156, 1264, 1498, 1525, 2542, 4100
BstPAI GACNNNNGTC 2 cut(s) 1582, 2480
BstSFI CTRYAG 7 cut(s) 873, 1697, 2444, 2543, 2883, 3441, 3482
BstSLI GKGCMC 2 cut(s) 1430, 4078
BstUI CGCG 1 cut(s) 88
BstV2I GAAGAC 3 cut(s) 890, 990, 2337
Bsu36I CCTNAGG 1 cut(s) 1593
BsuI GTATCC 1 cut(s) 109
BsuRI GGCC 7 cut(s) 22, 331, 600, 2839, 2864, 2889, 3667
BtsCI GGATG 4 cut(s) 55, 768, 2850, 3057
BtsI GCAGTG 2 cut(s) 882, 2441
BtsIMutI CAGTG 8 cut(s) 402, 882, 2432, 2441, 2491, 2818, 3376, 3754
BveI ACCTGC 2 cut(s) 2440, 3401
CaiI CAGNNNCTG 1 cut(s) 2764
CciI TCATGA 4 cut(s) 738, 1896, 2242, 3505
CfoI GCGC 2 cut(s) 2037, 3085
Cfr13I GGNCC 5 cut(s) 330, 2452, 2862, 3178, 3242
CsiI ACCWGGT 2 cut(s) 2077, 3421
Csp6I GTAC 6 cut(s) 524, 712, 1473, 1909, 1933, 4080
CviQI GTAC 6 cut(s) 524, 712, 1473, 1909, 1933, 4080
DriI GACNNNNNGTC 2 cut(s) 3474, 3637
EaeI YGGCCR 1 cut(s) 598
Eam1104I CTCTTC 2 cut(s) 119, 937
Eam1105I GACNNNNNGTC 2 cut(s) 3474, 3637
EarI CTCTTC 2 cut(s) 119, 937
EciI GGCGGA 1 cut(s) 450
Ecl136II GAGCTC 1 cut(s) 3173
Eco130I CCWWGG 4 cut(s) 1468, 1533, 3668, 4042
Eco147I AGGCCT 2 cut(s) 22, 3667
Eco24I GRGCYC 4 cut(s) 510, 1798, 2465, 3175
Eco31I GGTCTC 1 cut(s) 260
Eco32I GATATC 2 cut(s) 3210, 3745
Eco47I GGWCC 3 cut(s) 2452, 3178, 3242
Eco53kI GAGCTC 1 cut(s) 3173
Eco57I CTGAAG 8 cut(s) 884, 932, 2030, 2549, 2945, 3804, 3846, 4108
Eco81I CCTNAGG 1 cut(s) 1593
EcoICRI GAGCTC 1 cut(s) 3173
EcoNI CCTNNNNNAGG 3 cut(s) 27, 339, 3096
EcoO109I RGGNCCY 3 cut(s) 330, 2452, 2862
EcoRI GAATTC 2 cut(s) 577, 694
EcoRV GATATC 2 cut(s) 3210, 3745
EcoT14I CCWWGG 4 cut(s) 1468, 1533, 3668, 4042
EcoT22I ATGCAT 1 cut(s) 1413
EcoT38I GRGCYC 4 cut(s) 510, 1798, 2465, 3175
ErhI CCWWGG 4 cut(s) 1468, 1533, 3668, 4042
Esp3I CGTCTC 4 cut(s) 891, 1799, 1824, 3657
FalI AAGNNNNNCTT 4 cut(s) 930, 962, 2084, 2116
FauNDI CATATG 2 cut(s) 789, 2193
FblI GTMKAC 1 cut(s) 3481
FokI GGATG 4 cut(s) 62, 755, 2857, 3044
FriOI GRGCYC 4 cut(s) 510, 1798, 2465, 3175
FspBI CTAG 7 cut(s) 894, 1823, 2067, 3042, 3435, 3549, 3669
GlaI GCGC 2 cut(s) 2036, 3084
GsaI CCCAGC 1 cut(s) 3138
GsuI CTGGAG 2 cut(s) 2051, 3656
HaeII RGCGCY 2 cut(s) 2038, 3086
HaeIII GGCC 7 cut(s) 22, 331, 600, 2839, 2864, 2889, 3667
HapII CCGG 3 cut(s) 144, 2921, 3950
HhaI GCGC 2 cut(s) 2037, 3085
Hin1I GRCGYC 1 cut(s) 1808
Hin6I GCGC 2 cut(s) 2035, 3083
HinP1I GCGC 2 cut(s) 2035, 3083
HincII GTYRAC 3 cut(s) 3007, 3612, 3642
HindII GTYRAC 3 cut(s) 3007, 3612, 3642
HpaII CCGG 3 cut(s) 144, 2921, 3950
HphI GGTGA 8 cut(s) 502, 822, 2795, 3039, 3081, 3336, 3461, 3803
Hpy166II GTNNAC 5 cut(s) 3007, 3482, 3612, 3642, 4076
Hpy8I GTNNAC 5 cut(s) 3007, 3482, 3612, 3642, 4076
HpyCH4IV ACGT 4 cut(s) 1808, 1817, 3650, 4078
HpySE526I ACGT 4 cut(s) 1808, 1817, 3650, 4078
Hsp92I GRCGYC 1 cut(s) 1808
HspAI GCGC 2 cut(s) 2035, 3083
LmnI GCTCC 5 cut(s) 66, 2032, 2184, 2494, 3170
MabI ACCWGGT 2 cut(s) 2077, 3421
MaeI CTAG 7 cut(s) 894, 1823, 2067, 3042, 3435, 3549, 3669
MaeII ACGT 4 cut(s) 1808, 1817, 3650, 4078
MfeI CAATTG 3 cut(s) 285, 2264, 2618
MhlI GDGCHC 8 cut(s) 510, 1430, 1798, 2465, 2491, 3175, 3815, 4078
MlyI GAGTC 5 cut(s) 511, 1564, 1961, 2470, 3647
MmeI TCCRAC 7 cut(s) 204, 517, 796, 2494, 3124, 3148, 3929
Mph1103I ATGCAT 1 cut(s) 1413
MroXI GAANNNNTTC 1 cut(s) 4024
MslI CAYNNNNRTG 2 cut(s) 1851, 3261
MspA1I CMGCKG 1 cut(s) 2764
MspI CCGG 3 cut(s) 144, 2921, 3950
MunI CAATTG 3 cut(s) 285, 2264, 2618
Mva1269I GAATGC 3 cut(s) 955, 2515, 2989
MvnI CGCG 1 cut(s) 88
NciI CCSGG 1 cut(s) 2922
NdeI CATATG 2 cut(s) 789, 2193
NheI GCTAGC 1 cut(s) 3041
NmuCI GTSAC 5 cut(s) 381, 1599, 2211, 2982, 3371
NsiI ATGCAT 1 cut(s) 1413
NspI RCATGY 6 cut(s) 1156, 1264, 1498, 1525, 2542, 4100
PaeI GCATGC 2 cut(s) 1498, 4100
PagI TCATGA 4 cut(s) 738, 1896, 2242, 3505
PasI CCCWGGG 1 cut(s) 3203
PceI AGGCCT 2 cut(s) 22, 3667
PcsI WCGNNNNNNNCGW 1 cut(s) 1814
PctI GAATGC 3 cut(s) 955, 2515, 2989
PdmI GAANNNNTTC 1 cut(s) 4024
PflFI GACNNNGTC 1 cut(s) 271
PflMI CCANNNNNTGG 2 cut(s) 1381, 3428
PfoI TCCNGGA 2 cut(s) 340, 2416
PleI GAGTC 5 cut(s) 511, 1563, 1961, 2470, 3646
PpsI GAGTC 5 cut(s) 511, 1563, 1961, 2470, 3646
Ppu21I YACGTR 1 cut(s) 4079
PpuMI RGGWCCY 1 cut(s) 2452
PshAI GACNNNNGTC 2 cut(s) 1582, 2480
PshBI ATTAAT 3 cut(s) 1379, 2130, 2556
Psp124BI GAGCTC 1 cut(s) 3175
Psp5II RGGWCCY 1 cut(s) 2452
PspFI CCCAGC 1 cut(s) 3134
PspPI GGNCC 5 cut(s) 330, 2452, 2862, 3178, 3242
PspPPI RGGWCCY 1 cut(s) 2452
PstI CTGCAG 5 cut(s) 877, 1701, 2448, 2547, 3445
PstNI CAGNNNCTG 1 cut(s) 2764
PsyI GACNNNGTC 1 cut(s) 271
PvuII CAGCTG 1 cut(s) 2764
RsaI GTAC 6 cut(s) 525, 713, 1474, 1910, 1934, 4081
RsaNI GTAC 6 cut(s) 524, 712, 1473, 1909, 1933, 4080
RseI CAYNNNNRTG 2 cut(s) 1851, 3261
SacI GAGCTC 1 cut(s) 3175
Sau96I GGNCC 5 cut(s) 330, 2452, 2862, 3178, 3242
SchI GAGTC 5 cut(s) 511, 1564, 1961, 2470, 3647
SduI GDGCHC 8 cut(s) 510, 1430, 1798, 2465, 2491, 3175, 3815, 4078
SexAI ACCWGGT 2 cut(s) 2077, 3421
SfcI CTRYAG 7 cut(s) 873, 1697, 2444, 2543, 2883, 3441, 3482
SinI GGWCC 3 cut(s) 2452, 3178, 3242
SmiMI CAYNNNNRTG 2 cut(s) 1851, 3261
SmlI CTYRAG 6 cut(s) 27, 167, 1489, 2000, 2229, 2636
SmoI CTYRAG 6 cut(s) 27, 167, 1489, 2000, 2229, 2636
SpeI ACTAGT 1 cut(s) 3548
SphI GCATGC 2 cut(s) 1498, 4100
SseBI AGGCCT 2 cut(s) 22, 3667
SspMI CTAG 7 cut(s) 894, 1823, 2067, 3042, 3435, 3549, 3669
SstI GAGCTC 1 cut(s) 3175
StuI AGGCCT 2 cut(s) 22, 3667
StyI CCWWGG 4 cut(s) 1468, 1533, 3668, 4042
TaiI ACGT 4 cut(s) 1811, 1820, 3653, 4081
TaqII GACCGA 1 cut(s) 3230
TatI WGTACW 1 cut(s) 1908
TauI GCSGC 7 cut(s) 91, 976, 1114, 2170, 3724, 3776, 4166
TscAI CASTG 8 cut(s) 402, 882, 2439, 2448, 2491, 2818, 3376, 3754
TseFI GTSAC 5 cut(s) 381, 1599, 2211, 2982, 3371
Tsp45I GTSAC 5 cut(s) 381, 1599, 2211, 2982, 3371
TspGWI ACGGA 2 cut(s) 250, 2379
TspRI CASTG 8 cut(s) 402, 882, 2439, 2448, 2491, 2818, 3376, 3754
Tth111I GACNNNGTC 1 cut(s) 271
Van91I CCANNNNNTGG 2 cut(s) 1381, 3428
VneI GTGCAC 1 cut(s) 4074
VpaK11BI GGWCC 3 cut(s) 2452, 3178, 3242
VspI ATTAAT 3 cut(s) 1379, 2130, 2556
XagI CCTNNNNNAGG 3 cut(s) 27, 339, 3096
XbaI TCTAGA 2 cut(s) 1822, 3434
XceI RCATGY 6 cut(s) 1156, 1264, 1498, 1525, 2542, 4100
XmaJI CCTAGG 1 cut(s) 3668
XmiI GTMKAC 1 cut(s) 3481
XmnI GAANNNNTTC 1 cut(s) 4024
XspI CTAG 7 cut(s) 894, 1823, 2067, 3042, 3435, 3549, 3669
ZraI GACGTC 1 cut(s) 1809
Zsp2I ATGCAT 1 cut(s) 1413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.