Rmu_sc0003080.1_g000018

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003080.1
OG0006914
Physical Location & Seq
Forward (+)
91286 .. 95335
4050 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1560 bp
atgcctgcacacctcgatgatgaagctggaagacctcttccacaagttgtagaaactgcaactaatgctggaatcaacgtagagagagtcttatatgaagaagttgttaaaaacccagaagataattcatatagtcatgatgcgtggggtggtacagcaactccatatagttctgatgatggtggagagggtggaagcggaacaggtggtggaggtgaaagatataaatatggacaatcttctgtgggtggaggattaaaccaatttacatgtgaatgtaattttgattataccacccaagatgaagaccacaaagtgagaacagctggtcatggtgctcaagagaagacccgatatgggaggaggactagaggtgcaactgatgatcaaagtaccccatctgatgtttcttcaattgccttaagttttgactctatcagtttaggcacagatcgcaatgggatctcaaatgaatctcatgaagccaacaatcaatttgcttccgcaagcactgttacttgtagcttcaaacaagatggtagtgggaatacgatccaagggatgtttgtattcggaagctctctggtggacaatggtaacaataacttcttggaaaccaagtccaaagccgattacttgccgtatggaatcgacttcccaaatggaacttctgggagattcacaaatggtaaaaatgttattgatctcctcggtgatcagcttaagcttcccttcattccaccatttcaagacccctccaccaagggaagtaaaattgttcatggtgtcaatcatgcctctggctcttctggtatcttagatgatacaggattagttgtgggtaatgcaacgagcttgagtcaacaagttaaggactttgaggcggtgacactggcagagttagaagcagagctagggtgcagtagctctcaatcgctaccaagctacttgattgttgtaggagttggtgggaatgattacatgctcaactatttccttaggaaattgtacctccgaatcagtattgaaaacttcactgcaaatctcatagactctctagctcagaaactacaggaattgtacattttgggaggtcgtaaattcgtgttaacgtcgctaatgccattaggctacagtcctgtgattaacaggccaaatcttattggcaccccatcagctctgaattacgcagctcagctctacaacgctcgcttgaagacattgctcgactctctcaaaactcacttatctgacttcaatttcgttttggttaaccaatataacattatatccaatctcacttactctcctccttccactacaggctttaaagacataagcaacccttgttgtatagtttccctctctcaaggtggaaatggaatatcatgcgatagaggtgggaaggcatgtgaagataggacggcttatatgtattttgatgggttacatccaacagaagctgtgaacgttcaaatagcaaccaaggcatatgcttctactcaccagggcgacgcttacccaatcaatgtcagccaacttgcccagcttcaaatttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

519

Amino Acids

55.7

Weight (kDa)

4.96

Isoelectric Point (pI)

32.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 189 - 494 2.2e-24 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1166
AciI CCGC 3 cut(s) 198, 504, 884
AclI AACGTT 1 cut(s) 1470
AclWI GGATC 2 cut(s) 470, 547
AcsI RAATTY 2 cut(s) 1100, 1554
AdeI CACNNNGTG 1 cut(s) 316
AfaI GTAC 4 cut(s) 154, 394, 1010, 1082
AfiI CCNNNNNNNGG 1 cut(s) 357
AflII CTTAAG 2 cut(s) 421, 722
AflIII ACRYGT 1 cut(s) 269
AgsI TTSAA 8 cut(s) 414, 529, 749, 1028, 1216, 1258, 1475, 1553
AjnI CCWGG 1 cut(s) 1506
AjuI GAANNNNNNNTTGG 2 cut(s) 652, 684
Alw21I GWGCWC 1 cut(s) 340
AlwI GGATC 2 cut(s) 470, 547
AlwNI CAGNNNCTG 1 cut(s) 1463
AoxI GGCC 1 cut(s) 1151
ApeKI GCWGC 1 cut(s) 1190
ApoI RAATTY 2 cut(s) 1100, 1554
ArsI GACNNNNNNTTYG 2 cut(s) 1244, 1276
AsuHPI GGTGA 4 cut(s) 227, 725, 898, 1496
AxyI CCTNAGG 1 cut(s) 998
BanI GGYRCC 1 cut(s) 1166
BbsI GAAGAC 4 cut(s) 37, 312, 353, 1223
Bbv12I GWGCWC 1 cut(s) 340
BbvI GCAGC 1 cut(s) 1202
BccI CCATC 5 cut(s) 173, 406, 530, 1180, 1436
BceAI ACGGC 2 cut(s) 625, 1440
BciT130I CCWGG 1 cut(s) 1508
BclI TGATCA 2 cut(s) 385, 715
BfaI CTAG 3 cut(s) 369, 914, 1058
BfmI CTRYAG 3 cut(s) 1070, 1132, 1320
BfrI CTTAAG 2 cut(s) 421, 722
BisI GCNGC 1 cut(s) 1191
BlpI GCTNAGC 1 cut(s) 1194
BlsI GCNGC 1 cut(s) 1192
Bme1390I CCNGG 1 cut(s) 1508
BmiI GGNNCC 1 cut(s) 1168
BmrFI CCNGG 1 cut(s) 1508
BmsI GCATC 1 cut(s) 130
BpiI GAAGAC 4 cut(s) 37, 312, 353, 1223
Bpu1102I GCTNAGC 1 cut(s) 1194
BpuEI CTTGAG 3 cut(s) 324, 877, 1353
BsaJI CCNNGG 5 cut(s) 556, 709, 762, 1485, 1507
BsaXI ACNNNNNCTCC 4 cut(s) 145, 175, 1291, 1321
Bsc4I CCNNNNNNNGG 1 cut(s) 357
Bse1I ACTGG 1 cut(s) 897
Bse21I CCTNAGG 1 cut(s) 998
Bse3DI GCAATG 2 cut(s) 463, 1220
BseBI CCWGG 1 cut(s) 1508
BseDI CCNNGG 5 cut(s) 556, 709, 762, 1485, 1507
BseGI GGATG 2 cut(s) 567, 1450
BseLI CCNNNNNNNGG 1 cut(s) 357
BseMI GCAATG 2 cut(s) 463, 1220
BseMII CTCAG 2 cut(s) 1076, 1208
BseNI ACTGG 1 cut(s) 897
BseRI GAGGAG 3 cut(s) 376, 698, 1299
BseXI GCAGC 1 cut(s) 1202
BseYI CCCAGC 1 cut(s) 1545
BsgI GTGCAG 1 cut(s) 940
BshFI GGCC 1 cut(s) 1153
BshNI GGYRCC 1 cut(s) 1166
BsiHKAI GWGCWC 1 cut(s) 340
BslI CCNNNNNNNGG 1 cut(s) 357
BsnI GGCC 1 cut(s) 1153
Bsp1286I GDGCHC 1 cut(s) 340
Bsp1407I TGTACA 1 cut(s) 1080
Bsp143I GATC 6 cut(s) 385, 451, 462, 552, 703, 715
Bsp1720I GCTNAGC 1 cut(s) 1194
BspACI CCGC 3 cut(s) 198, 504, 884
BspANI GGCC 1 cut(s) 1153
BspCNI CTCAG 2 cut(s) 1075, 1207
BspHI TCATGA 2 cut(s) 136, 478
BspLI GGNNCC 1 cut(s) 1168
BspPI GGATC 2 cut(s) 470, 547
BspQI GCTCTTC 1 cut(s) 811
BspT107I GGYRCC 1 cut(s) 1166
BspTI CTTAAG 2 cut(s) 421, 722
BsrDI GCAATG 2 cut(s) 463, 1220
BsrGI TGTACA 1 cut(s) 1080
BsrI ACTGG 1 cut(s) 897
BssECI CCNNGG 5 cut(s) 556, 709, 762, 1485, 1507
BssMI GATC 6 cut(s) 385, 451, 462, 552, 703, 715
BssT1I CCWWGG 3 cut(s) 556, 762, 1485
Bst2UI CCWGG 1 cut(s) 1508
Bst4CI ACNGT 2 cut(s) 514, 1136
Bst6I CTCTTC 2 cut(s) 42, 811
BstAFI CTTAAG 2 cut(s) 421, 722
BstAPI GCANNNNNTGC 1 cut(s) 65
BstAUI TGTACA 1 cut(s) 1080
BstC8I GCNNGC 3 cut(s) 6, 508, 1210
BstDEI CTNAG 4 cut(s) 817, 998, 1062, 1194
BstF5I GGATG 2 cut(s) 567, 1450
BstKTI GATC 6 cut(s) 388, 454, 465, 555, 706, 718
BstMBI GATC 6 cut(s) 385, 451, 462, 552, 703, 715
BstMWI GCNNNNNNNGC 3 cut(s) 65, 453, 1487
BstNI CCWGG 1 cut(s) 1508
BstNSI RCATGY 3 cut(s) 273, 985, 1413
BstSCI CCNGG 1 cut(s) 1506
BstSFI CTRYAG 3 cut(s) 1070, 1132, 1320
BstV1I GCAGC 1 cut(s) 1202
BstV2I GAAGAC 4 cut(s) 37, 312, 353, 1223
BstX2I RGATCY 1 cut(s) 462
BstYI RGATCY 1 cut(s) 462
Bsu36I CCTNAGG 1 cut(s) 998
BsuRI GGCC 1 cut(s) 1153
BtsCI GGATG 2 cut(s) 567, 1450
BtsI GCAGTG 1 cut(s) 1035
BtsIMutI CAGTG 3 cut(s) 510, 890, 1035
Cac8I GCNNGC 3 cut(s) 6, 508, 1210
CaiI CAGNNNCTG 1 cut(s) 1463
CciI TCATGA 2 cut(s) 136, 478
CseI GACGC 1 cut(s) 1523
Csp6I GTAC 4 cut(s) 153, 393, 1009, 1081
CviAII CATG 9 cut(s) 137, 270, 332, 479, 782, 794, 982, 1389, 1410
CviQI GTAC 4 cut(s) 153, 393, 1009, 1081
DdeI CTNAG 4 cut(s) 817, 998, 1062, 1194
DpnI GATC 6 cut(s) 387, 453, 464, 554, 705, 717
DpnII GATC 6 cut(s) 385, 451, 462, 552, 703, 715
DraI TTTAAA 1 cut(s) 1330
DraIII CACNNNGTG 1 cut(s) 316
Eam1104I CTCTTC 2 cut(s) 42, 811
EarI CTCTTC 2 cut(s) 42, 811
Eco130I CCWWGG 3 cut(s) 556, 762, 1485
Eco81I CCTNAGG 1 cut(s) 998
EcoRII CCWGG 1 cut(s) 1506
EcoT14I CCWWGG 3 cut(s) 556, 762, 1485
ErhI CCWWGG 3 cut(s) 556, 762, 1485
FaeI CATG 9 cut(s) 140, 273, 335, 482, 785, 797, 985, 1392, 1413
FalI AAGNNNNNCTT 4 cut(s) 716, 748, 1330, 1362
FatI CATG 9 cut(s) 136, 269, 331, 478, 781, 793, 981, 1388, 1409
FauNDI CATATG 1 cut(s) 1492
FbaI TGATCA 2 cut(s) 385, 715
Fnu4HI GCNGC 1 cut(s) 1191
FokI GGATG 2 cut(s) 574, 1437
Fsp4HI GCNGC 1 cut(s) 1191
FspBI CTAG 3 cut(s) 369, 914, 1058
GluI GCNGC 1 cut(s) 1191
GsaI CCCAGC 1 cut(s) 1549
HaeIII GGCC 1 cut(s) 1153
HgaI GACGC 1 cut(s) 1523
Hin1II CATG 9 cut(s) 140, 273, 335, 482, 785, 797, 985, 1392, 1413
HincII GTYRAC 3 cut(s) 863, 1110, 1273
HindII GTYRAC 3 cut(s) 863, 1110, 1273
HindIII AAGCTT 1 cut(s) 725
HpaI GTTAAC 2 cut(s) 1110, 1273
HphI GGTGA 4 cut(s) 227, 725, 898, 1496
Hpy166II GTNNAC 5 cut(s) 589, 863, 1110, 1273, 1468
Hpy188I TCNGA 7 cut(s) 175, 403, 575, 1016, 1065, 1182, 1252
Hpy188III TCNNGA 4 cut(s) 137, 341, 479, 749
Hpy8I GTNNAC 5 cut(s) 589, 863, 1110, 1273, 1468
Hpy99I CGWCG 2 cut(s) 1117, 1517
HpyAV CCTTC 3 cut(s) 742, 1323, 1399
HpyCH4III ACNGT 2 cut(s) 514, 1136
HpyCH4IV ACGT 3 cut(s) 78, 1112, 1470
HpyCH4V TGCA 6 cut(s) 8, 59, 377, 848, 921, 1040
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 453, 1487
HpyF3I CTNAG 4 cut(s) 817, 998, 1062, 1194
HpySE526I ACGT 3 cut(s) 78, 1112, 1470
Hsp92II CATG 9 cut(s) 140, 273, 335, 482, 785, 797, 985, 1392, 1413
Ksp22I TGATCA 2 cut(s) 385, 715
KspAI GTTAAC 2 cut(s) 1110, 1273
Kzo9I GATC 6 cut(s) 385, 451, 462, 552, 703, 715
LguI GCTCTTC 1 cut(s) 811
Lsp1109I GCAGC 1 cut(s) 1202
LweI GCATC 1 cut(s) 130
MaeI CTAG 3 cut(s) 369, 914, 1058
MaeII ACGT 3 cut(s) 78, 1112, 1470
MaeIII GTNAC 4 cut(s) 514, 596, 886, 1446
MalI GATC 6 cut(s) 387, 453, 464, 554, 705, 717
MboI GATC 6 cut(s) 385, 451, 462, 552, 703, 715
MfeI CAATTG 1 cut(s) 414
MflI RGATCY 1 cut(s) 462
MhlI GDGCHC 1 cut(s) 340
MlyI GAGTC 5 cut(s) 96, 425, 868, 1046, 1223
MmeI TCCRAC 1 cut(s) 1478
MslI CAYNNNNRTG 2 cut(s) 15, 274
MspA1I CMGCKG 1 cut(s) 326
MspCI CTTAAG 2 cut(s) 421, 722
MspR9I CCNGG 1 cut(s) 1508
MunI CAATTG 1 cut(s) 414
MvaI CCWGG 1 cut(s) 1508
MwoI GCNNNNNNNGC 3 cut(s) 65, 453, 1487
NdeI CATATG 1 cut(s) 1492
NdeII GATC 6 cut(s) 385, 451, 462, 552, 703, 715
NlaIII CATG 9 cut(s) 140, 273, 335, 482, 785, 797, 985, 1392, 1413
NlaIV GGNNCC 1 cut(s) 1168
NmuCI GTSAC 1 cut(s) 886
NspI RCATGY 3 cut(s) 273, 985, 1413
PagI TCATGA 2 cut(s) 136, 478
PciI ACATGT 1 cut(s) 269
PciSI GCTCTTC 1 cut(s) 811
PfeI GAWTC 5 cut(s) 72, 473, 648, 678, 1017
PkrI GCNGC 1 cut(s) 1192
PleI GAGTC 5 cut(s) 95, 425, 867, 1046, 1223
PpsI GAGTC 5 cut(s) 95, 425, 867, 1046, 1223
PscI ACATGT 1 cut(s) 269
Psp1406I AACGTT 1 cut(s) 1470
Psp6I CCWGG 1 cut(s) 1506
PspFI CCCAGC 1 cut(s) 1545
PspGI CCWGG 1 cut(s) 1506
PspN4I GGNNCC 1 cut(s) 1168
PstNI CAGNNNCTG 1 cut(s) 1463
PsuI RGATCY 1 cut(s) 462
PvuII CAGCTG 1 cut(s) 326
RsaI GTAC 4 cut(s) 154, 394, 1010, 1082
RsaNI GTAC 4 cut(s) 153, 393, 1009, 1081
RseI CAYNNNNRTG 2 cut(s) 15, 274
SapI GCTCTTC 1 cut(s) 811
SatI GCNGC 1 cut(s) 1191
Sau3AI GATC 6 cut(s) 385, 451, 462, 552, 703, 715
SchI GAGTC 5 cut(s) 96, 425, 868, 1046, 1223
ScrFI CCNGG 1 cut(s) 1508
SduI GDGCHC 1 cut(s) 340
SfaNI GCATC 1 cut(s) 130
SfcI CTRYAG 3 cut(s) 1070, 1132, 1320
SmiMI CAYNNNNRTG 2 cut(s) 15, 274
SmlI CTYRAG 5 cut(s) 339, 421, 722, 856, 1368
SmoI CTYRAG 5 cut(s) 339, 421, 722, 856, 1368
SsiI CCGC 3 cut(s) 198, 504, 884
SspMI CTAG 3 cut(s) 369, 914, 1058
StyD4I CCNGG 1 cut(s) 1506
StyI CCWWGG 3 cut(s) 556, 762, 1485
TaaI ACNGT 2 cut(s) 514, 1136
TaiI ACGT 3 cut(s) 81, 1115, 1473
TaqI TCGA 3 cut(s) 15, 651, 1227
TatI WGTACW 1 cut(s) 1080
TfiI GAWTC 5 cut(s) 72, 473, 648, 678, 1017
TscAI CASTG 3 cut(s) 517, 897, 1042
TseFI GTSAC 1 cut(s) 886
TseI GCWGC 1 cut(s) 1190
Tsp45I GTSAC 1 cut(s) 886
TspDTI ATGAA 8 cut(s) 36, 111, 117, 318, 486, 495, 724, 770
TspRI CASTG 3 cut(s) 517, 897, 1042
Vha464I CTTAAG 2 cut(s) 421, 722
XapI RAATTY 2 cut(s) 1100, 1554
XceI RCATGY 3 cut(s) 273, 985, 1413
XspI CTAG 3 cut(s) 369, 914, 1058
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.