Rmu_sc0003130.1_g000020

Predicted membrane protein (DUF2232)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003130.1
OG0009555
Physical Location & Seq
Forward (+)
91560 .. 94116
2557 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 801 bp
atgttattctcattcgtcgtttctcctttgcaaaaacccaaccccttcaccttctccccaatttcactgcccaaacccaccctcaagctctcaagggcgtcgcaaagtcgatcgcttttcttcggaaatgaggaggacaccgccggaatttcaactgacctcgacgacttgtccccaaacggcgtcgttcaccggaaaacattggcattggttgagtgctccatgtttgctgcgcttactgggttggtgtatttcctcagcaattctctcgcaattgagaattactttggttgcttcttttcgctgcccatagtgatcacctcaatgagatggggcaccgcagctggaagaaaaactatggtggcaacgactatgctattacttgtcttgtctggtccggtgaaagccttaacctatttgcttaagcatggattagttggctttacaatgggaagtttgtggaggtcgggagcaaattggactatttcagttttcttgtgcgcaattgttcgagcattgggttctgtagggtatgttttgatcacctctttcttgatcagagaaaacatatttgctctgatcaccataaacattcatgcttctctaacattcatcttcacagcagcaggcatctatacagttccatcgatggaagcaatatatgccttatttagtattctggtttttataaatagtgggtccttcacgttcttgctgcacatactgtatggtgccttcctttcgaggctcgggatgaaagcatctttgagattgccaagatggctggagaaggcaatatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2232

Protein Analysis

266

Amino Acids

29.16

Weight (kDa)

9.71

Isoelectric Point (pI)

39.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2232 PF09991 98 - 253 1.3e-07 Predicted membrane protein (DUF2232)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 689
Acc16I TGCGCA 1 cut(s) 502
AccB1I GGYRCC 2 cut(s) 335, 731
AciI CCGC 2 cut(s) 141, 339
AcsI RAATTY 1 cut(s) 147
AcyI GRCGYC 2 cut(s) 98, 183
AflII CTTAAG 1 cut(s) 422
AgsI TTSAA 1 cut(s) 153
AhdI GACNNNNNGTC 1 cut(s) 169
AluBI AGCT 2 cut(s) 88, 344
AluI AGCT 2 cut(s) 88, 344
Alw21I GWGCWC 1 cut(s) 221
Ama87I CYCGRG 1 cut(s) 749
ApeKI GCWGC 5 cut(s) 230, 304, 341, 623, 715
ApoI RAATTY 1 cut(s) 147
AspLEI GCGC 2 cut(s) 235, 503
AspS9I GGNCC 2 cut(s) 395, 699
AsuHPI GGTGA 6 cut(s) 40, 182, 310, 412, 535, 574
AvaI CYCGRG 1 cut(s) 749
AvaII GGWCC 2 cut(s) 395, 699
BaeGI GKGCMC 1 cut(s) 338
BanI GGYRCC 2 cut(s) 335, 731
BarI GAAGNNNNNNTAC 2 cut(s) 719, 751
Bbv12I GWGCWC 1 cut(s) 221
BbvCI CCTCAGC 1 cut(s) 257
BbvI GCAGC 5 cut(s) 217, 291, 353, 635, 702
BccI CCATC 4 cut(s) 324, 643, 652, 774
BceAI ACGGC 1 cut(s) 196
BcgI CGANNNNNNTGC 2 cut(s) 250, 284
BclI TGATCA 4 cut(s) 315, 540, 555, 579
BfmI CTRYAG 1 cut(s) 525
BfrI CTTAAG 1 cut(s) 422
BglI GCCNNNNNGGC 1 cut(s) 781
BisI GCNGC 5 cut(s) 231, 305, 342, 624, 716
BlsI GCNGC 5 cut(s) 232, 306, 343, 625, 717
Bme18I GGWCC 2 cut(s) 395, 699
BmeRI GACNNNNNGTC 1 cut(s) 169
BmeT110I CYCGRG 1 cut(s) 749
BmgT120I GGNCC 2 cut(s) 395, 699
BmiI GGNNCC 3 cut(s) 337, 700, 733
BmrI ACTGGG 1 cut(s) 249
BmsI GCATC 2 cut(s) 639, 770
BmuI ACTGGG 1 cut(s) 249
Bpu10I CCTNAGC 1 cut(s) 257
BpuEI CTTGAG 2 cut(s) 68, 76
Bsa29I ATCGAT 1 cut(s) 647
BsaHI GRCGYC 2 cut(s) 98, 183
BsaWI WCCGGW 2 cut(s) 192, 397
Bse1I ACTGG 1 cut(s) 244
BseCI ATCGAT 1 cut(s) 647
BseGI GGATG 1 cut(s) 759
BseMII CTCAG 1 cut(s) 271
BseNI ACTGG 1 cut(s) 244
BseRI GAGGAG 1 cut(s) 146
BseSI GKGCMC 1 cut(s) 338
BseXI GCAGC 5 cut(s) 217, 291, 353, 635, 702
BsgI GTGCAG 1 cut(s) 701
Bsh1285I CGRYCG 1 cut(s) 113
BshNI GGYRCC 2 cut(s) 335, 731
BshVI ATCGAT 1 cut(s) 647
BsiEI CGRYCG 1 cut(s) 113
BsiHKAI GWGCWC 1 cut(s) 221
BsiHKCI CYCGRG 1 cut(s) 749
BsiSI CCGG 3 cut(s) 144, 193, 398
BslFI GGGAC 1 cut(s) 157
BsmFI GGGAC 1 cut(s) 157
BsoBI CYCGRG 1 cut(s) 749
Bsp1286I GDGCHC 2 cut(s) 221, 338
Bsp143I GATC 5 cut(s) 110, 315, 540, 555, 579
BspACI CCGC 2 cut(s) 141, 339
BspCNI CTCAG 1 cut(s) 270
BspDI ATCGAT 1 cut(s) 647
BspLI GGNNCC 3 cut(s) 337, 700, 733
BspT107I GGYRCC 2 cut(s) 335, 731
BspTI CTTAAG 1 cut(s) 422
BsrI ACTGG 1 cut(s) 244
BssMI GATC 5 cut(s) 110, 315, 540, 555, 579
BssNI GRCGYC 2 cut(s) 98, 183
Bst4CI ACNGT 2 cut(s) 640, 726
BstACI GRCGYC 2 cut(s) 98, 183
BstAFI CTTAAG 1 cut(s) 422
BstAPI GCANNNNNTGC 1 cut(s) 662
BstC8I GCNNGC 1 cut(s) 628
BstDEI CTNAG 1 cut(s) 257
BstF5I GGATG 1 cut(s) 759
BstHHI GCGC 2 cut(s) 235, 503
BstKTI GATC 5 cut(s) 113, 318, 543, 558, 582
BstMBI GATC 5 cut(s) 110, 315, 540, 555, 579
BstMCI CGRYCG 1 cut(s) 113
BstMWI GCNNNNNNNGC 2 cut(s) 662, 781
BstSFI CTRYAG 1 cut(s) 525
BstSLI GKGCMC 1 cut(s) 338
BstV1I GCAGC 5 cut(s) 217, 291, 353, 635, 702
Bsu15I ATCGAT 1 cut(s) 647
BsuTUI ATCGAT 1 cut(s) 647
BtsCI GGATG 1 cut(s) 759
BtsI GCAGTG 1 cut(s) 65
BtsIMutI CAGTG 1 cut(s) 65
Cac8I GCNNGC 1 cut(s) 628
CfoI GCGC 2 cut(s) 235, 503
Cfr13I GGNCC 2 cut(s) 395, 699
ClaI ATCGAT 1 cut(s) 647
CseI GACGC 2 cut(s) 87, 172
CviAII CATG 3 cut(s) 223, 428, 596
CviJI RGCY 6 cut(s) 88, 344, 407, 441, 748, 784
CviKI_1 RGCY 6 cut(s) 88, 344, 407, 441, 748, 784
DdeI CTNAG 1 cut(s) 257
DpnI GATC 5 cut(s) 112, 317, 542, 557, 581
DpnII GATC 5 cut(s) 110, 315, 540, 555, 579
DriI GACNNNNNGTC 1 cut(s) 169
Eam1105I GACNNNNNGTC 1 cut(s) 169
Eco47I GGWCC 2 cut(s) 395, 699
Eco88I CYCGRG 1 cut(s) 749
EcoO109I RGGNCCY 1 cut(s) 699
FaeI CATG 3 cut(s) 226, 431, 599
FaqI GGGAC 1 cut(s) 157
FatI CATG 3 cut(s) 222, 427, 595
FbaI TGATCA 4 cut(s) 315, 540, 555, 579
Fnu4HI GCNGC 5 cut(s) 231, 305, 342, 624, 716
FokI GGATG 1 cut(s) 766
Fsp4HI GCNGC 5 cut(s) 231, 305, 342, 624, 716
FspI TGCGCA 1 cut(s) 502
GlaI GCGC 2 cut(s) 234, 502
GluI GCNGC 5 cut(s) 231, 305, 342, 624, 716
HapII CCGG 3 cut(s) 144, 193, 398
HgaI GACGC 2 cut(s) 87, 172
HhaI GCGC 2 cut(s) 235, 503
Hin1I GRCGYC 2 cut(s) 98, 183
Hin1II CATG 3 cut(s) 226, 431, 599
Hin6I GCGC 2 cut(s) 233, 501
HinP1I GCGC 2 cut(s) 233, 501
HpaII CCGG 3 cut(s) 144, 193, 398
HphI GGTGA 6 cut(s) 40, 182, 310, 412, 535, 574
Hpy166II GTNNAC 1 cut(s) 190
Hpy188I TCNGA 3 cut(s) 125, 560, 579
Hpy188III TCNNGA 3 cut(s) 468, 553, 751
Hpy8I GTNNAC 1 cut(s) 190
Hpy99I CGWCG 4 cut(s) 20, 103, 167, 188
HpyAV CCTTC 5 cut(s) 55, 61, 712, 745, 784
HpyCH4III ACNGT 2 cut(s) 640, 726
HpyCH4IV ACGT 1 cut(s) 707
HpyCH4V TGCA 2 cut(s) 31, 718
HpyF10VI GCNNNNNNNGC 2 cut(s) 662, 781
HpyF3I CTNAG 1 cut(s) 257
HpySE526I ACGT 1 cut(s) 707
Hsp92I GRCGYC 2 cut(s) 98, 183
Hsp92II CATG 3 cut(s) 226, 431, 599
HspAI GCGC 2 cut(s) 233, 501
Ksp22I TGATCA 4 cut(s) 315, 540, 555, 579
Kzo9I GATC 5 cut(s) 110, 315, 540, 555, 579
LmnI GCTCC 2 cut(s) 224, 470
LpnPI CCDG 9 cut(s) 157, 206, 225, 330, 378, 411, 612, 665, 770
Lsp1109I GCAGC 5 cut(s) 217, 291, 353, 635, 702
LweI GCATC 2 cut(s) 639, 770
MaeII ACGT 1 cut(s) 707
MalI GATC 5 cut(s) 112, 317, 542, 557, 581
MboI GATC 5 cut(s) 110, 315, 540, 555, 579
MboII GAAGA 3 cut(s) 112, 360, 607
MfeI CAATTG 2 cut(s) 273, 504
MhlI GDGCHC 2 cut(s) 221, 338
MluCI AATT 7 cut(s) 60, 147, 262, 273, 280, 475, 504
MnlI CCTC 9 cut(s) 92, 124, 127, 170, 266, 331, 456, 556, 738
MseI TTAA 2 cut(s) 410, 423
MslI CAYNNNNRTG 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 344
MspCI CTTAAG 1 cut(s) 422
MspI CCGG 3 cut(s) 144, 193, 398
MunI CAATTG 2 cut(s) 273, 504
MwoI GCNNNNNNNGC 2 cut(s) 662, 781
NdeII GATC 5 cut(s) 110, 315, 540, 555, 579
NlaIII CATG 3 cut(s) 226, 431, 599
NlaIV GGNNCC 3 cut(s) 337, 700, 733
NsbI TGCGCA 1 cut(s) 502
PkrI GCNGC 5 cut(s) 232, 306, 343, 625, 717
Ple19I CGATCG 1 cut(s) 113
PpuMI RGGWCCY 1 cut(s) 699
PsiI TTATAA 1 cut(s) 689
Psp5II RGGWCCY 1 cut(s) 699
PspN4I GGNNCC 3 cut(s) 337, 700, 733
PspPI GGNCC 2 cut(s) 395, 699
PspPPI RGGWCCY 1 cut(s) 699
PvuI CGATCG 1 cut(s) 113
PvuII CAGCTG 1 cut(s) 344
RseI CAYNNNNRTG 1 cut(s) 323
SaqAI TTAA 2 cut(s) 410, 423
SatI GCNGC 5 cut(s) 231, 305, 342, 624, 716
Sau3AI GATC 5 cut(s) 110, 315, 540, 555, 579
Sau96I GGNCC 2 cut(s) 395, 699
SduI GDGCHC 2 cut(s) 221, 338
SetI ASST 9 cut(s) 53, 90, 162, 323, 346, 416, 467, 548, 710
SfaNI GCATC 2 cut(s) 639, 770
SfcI CTRYAG 1 cut(s) 525
SinI GGWCC 2 cut(s) 395, 699
SmiMI CAYNNNNRTG 1 cut(s) 323
SmlI CTYRAG 3 cut(s) 83, 91, 422
SmoI CTYRAG 3 cut(s) 83, 91, 422
Sse9I AATT 7 cut(s) 60, 147, 262, 273, 280, 475, 504
SsiI CCGC 2 cut(s) 141, 339
TaaI ACNGT 2 cut(s) 640, 726
TaiI ACGT 1 cut(s) 710
TaqI TCGA 5 cut(s) 109, 162, 511, 647, 743
TasI AATT 7 cut(s) 60, 147, 262, 273, 280, 475, 504
Tru1I TTAA 2 cut(s) 410, 423
Tru9I TTAA 2 cut(s) 410, 423
TscAI CASTG 1 cut(s) 72
TseI GCWGC 5 cut(s) 230, 304, 341, 623, 715
TspDTI ATGAA 3 cut(s) 584, 601, 770
TspRI CASTG 1 cut(s) 72
Vha464I CTTAAG 1 cut(s) 422
VpaK11BI GGWCC 2 cut(s) 395, 699
XapI RAATTY 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.