Rmu_sc0003152.1_g000001

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003152.1
OG0014716
Physical Location & Seq
Reverse (-)
2 .. 888
887 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 887 bp
atggataagagctggataaaatataagtcaaatagtgcagagtaccgagaaggtgttagacaatttgtcttgaattccaagcggtatgcaaggaatcctgagatgattatttgtccttgcgtaatttgtcgaaaccttagcccacaaactgatgatgacttggtcatacatttgatgagatacggcgttgatcctggatacgatatttggtgtgcacatggtgaagatgcaagtacatctgtccaagttgaagattgtgaaatgtcagatcactttgatgtggattatgaacttccagaagtgcatcaaatgtataaggacgcatgcttccctttttctgatggagctggaagctcaaggactataaatatagaggaagagtataaaaagaaagtggaggaggctgaagttcctctatttccagggtgtaagaagcacacaaaactatcagccacccttgtattgtacaaattcaaagctgccaatggccttgctaactcagcttttgatgaattgctgttggaaatcaaagacatgctaccagaagttaatacttttcctgaatttttgtacacaataaaaaagtttttgaaaggatttgatttagggtatgagaagattcatgcatgcatcaacgactgctgcctgttcagaaaagagaaagagcacatggagagatgtcccaaatgtgatgcttcgaggtggaaagcaaattcacggactaaaaagataaataagggagttcctgcaaaggtcctgcgctactttcctatagttccaaggattcaaaggatgtttctttcagcaaaaataggtgagcaattaacttggcactctactcatcacagtcaagatggcatgatgcgtcatccagttgactctattca
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218 Transpos_assoc Transposase_21

Protein Analysis

295

Amino Acids

34.12

Weight (kDa)

6.82

Isoelectric Point (pI)

40.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transpos_assoc PF13963 3 - 75 1.1e-16 Transposase-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 82
AclWI GGATC 1 cut(s) 185
AcsI RAATTY 4 cut(s) 73, 470, 563, 712
AcuI CTGAAG 1 cut(s) 426
AdeI CACNNNGTG 1 cut(s) 221
AfaI GTAC 4 cut(s) 44, 235, 467, 572
AgsI TTSAA 5 cut(s) 73, 251, 475, 592, 788
AhdI GACNNNNNGTC 1 cut(s) 65
AjnI CCWGG 2 cut(s) 193, 421
AluBI AGCT 5 cut(s) 12, 347, 354, 479, 503
AluI AGCT 5 cut(s) 12, 347, 354, 479, 503
Alw21I GWGCWC 2 cut(s) 217, 669
Alw44I GTGCAC 1 cut(s) 213
AlwI GGATC 1 cut(s) 185
AoxI GGCC 1 cut(s) 487
ApaLI GTGCAC 1 cut(s) 213
ApeKI GCWGC 2 cut(s) 479, 642
ApoI RAATTY 4 cut(s) 73, 470, 563, 712
AspLEI GCGC 1 cut(s) 762
AspS9I GGNCC 1 cut(s) 754
AsuHPI GGTGA 2 cut(s) 233, 827
AvaII GGWCC 1 cut(s) 754
BaeGI GKGCMC 1 cut(s) 217
Bbv12I GWGCWC 2 cut(s) 217, 669
BbvI GCAGC 2 cut(s) 466, 629
BccI CCATC 2 cut(s) 335, 848
BceAI ACGGC 1 cut(s) 199
BciT130I CCWGG 2 cut(s) 195, 423
BciVI GTATCC 1 cut(s) 191
BfmI CTRYAG 1 cut(s) 771
BfuI GTATCC 1 cut(s) 191
BisI GCNGC 2 cut(s) 480, 643
BlsI GCNGC 2 cut(s) 481, 644
Bme1390I CCNGG 2 cut(s) 195, 423
Bme18I GGWCC 1 cut(s) 754
BmeRI GACNNNNNGTC 1 cut(s) 65
BmgT120I GGNCC 1 cut(s) 754
BmrFI CCNGG 2 cut(s) 195, 423
BmsI GCATC 5 cut(s) 217, 313, 639, 682, 852
Bpu10I CCTNAGC 1 cut(s) 137
BpuEI CTTGAG 1 cut(s) 340
BsaJI CCNNGG 2 cut(s) 422, 779
Bse1I ACTGG 1 cut(s) 872
BseBI CCWGG 2 cut(s) 195, 423
BseDI CCNNGG 2 cut(s) 422, 779
BseGI GGATG 2 cut(s) 798, 868
BseMII CTCAG 2 cut(s) 90, 513
BseNI ACTGG 1 cut(s) 872
BseRI GAGGAG 1 cut(s) 413
BseSI GKGCMC 1 cut(s) 217
BseXI GCAGC 2 cut(s) 466, 629
BsgI GTGCAG 1 cut(s) 57
BshFI GGCC 1 cut(s) 489
BsiHKAI GWGCWC 2 cut(s) 217, 669
BslFI GGGAC 1 cut(s) 666
BsmFI GGGAC 1 cut(s) 666
BsnI GGCC 1 cut(s) 489
Bsp1286I GDGCHC 2 cut(s) 217, 669
Bsp1407I TGTACA 2 cut(s) 465, 570
Bsp143I GATC 2 cut(s) 190, 268
BspACI CCGC 1 cut(s) 82
BspANI GGCC 1 cut(s) 489
BspCNI CTCAG 2 cut(s) 91, 512
BspPI GGATC 1 cut(s) 185
BsrGI TGTACA 2 cut(s) 465, 570
BsrI ACTGG 1 cut(s) 872
BssECI CCNNGG 2 cut(s) 422, 779
BssMI GATC 2 cut(s) 190, 268
BssT1I CCWWGG 1 cut(s) 779
Bst2UI CCWGG 2 cut(s) 195, 423
Bst4CI ACNGT 1 cut(s) 848
Bst6I CTCTTC 1 cut(s) 372
BstAUI TGTACA 2 cut(s) 465, 570
BstC8I GCNNGC 2 cut(s) 325, 628
BstDEI CTNAG 3 cut(s) 99, 137, 499
BstF5I GGATG 2 cut(s) 798, 868
BstHHI GCGC 1 cut(s) 762
BstKTI GATC 2 cut(s) 193, 271
BstMBI GATC 2 cut(s) 190, 268
BstMWI GCNNNNNNNGC 1 cut(s) 500
BstNI CCWGG 2 cut(s) 195, 423
BstNSI RCATGY 3 cut(s) 327, 538, 630
BstSCI CCNGG 2 cut(s) 193, 421
BstSFI CTRYAG 1 cut(s) 771
BstSLI GKGCMC 1 cut(s) 217
BstV1I GCAGC 2 cut(s) 466, 629
BsuI GTATCC 1 cut(s) 191
BsuRI GGCC 1 cut(s) 489
BtsCI GGATG 2 cut(s) 798, 868
Cac8I GCNNGC 2 cut(s) 325, 628
CfoI GCGC 1 cut(s) 762
Cfr13I GGNCC 1 cut(s) 754
CseI GACGC 2 cut(s) 329, 854
Csp6I GTAC 4 cut(s) 43, 234, 466, 571
CviAII CATG 7 cut(s) 218, 324, 535, 623, 627, 670, 859
CviJI RGCY 9 cut(s) 12, 141, 347, 354, 404, 452, 479, 489, 503
CviKI_1 RGCY 9 cut(s) 12, 141, 347, 354, 404, 452, 479, 489, 503
CviQI GTAC 4 cut(s) 43, 234, 466, 571
DdeI CTNAG 3 cut(s) 99, 137, 499
DpnI GATC 2 cut(s) 192, 270
DpnII GATC 2 cut(s) 190, 268
DraIII CACNNNGTG 1 cut(s) 221
DriI GACNNNNNGTC 1 cut(s) 65
Eam1104I CTCTTC 1 cut(s) 372
Eam1105I GACNNNNNGTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 372
Eco130I CCWWGG 1 cut(s) 779
Eco47I GGWCC 1 cut(s) 754
Eco57I CTGAAG 1 cut(s) 426
EcoO109I RGGNCCY 1 cut(s) 754
EcoRI GAATTC 1 cut(s) 73
EcoRII CCWGG 2 cut(s) 193, 421
EcoT14I CCWWGG 1 cut(s) 779
EcoT22I ATGCAT 2 cut(s) 628, 632
ErhI CCWWGG 1 cut(s) 779
FaeI CATG 7 cut(s) 221, 327, 538, 626, 630, 673, 862
FaqI GGGAC 1 cut(s) 666
FatI CATG 7 cut(s) 217, 323, 534, 622, 626, 669, 858
Fnu4HI GCNGC 2 cut(s) 480, 643
FokI GGATG 2 cut(s) 805, 855
Fsp4HI GCNGC 2 cut(s) 480, 643
GlaI GCGC 1 cut(s) 761
GluI GCNGC 2 cut(s) 480, 643
HaeIII GGCC 1 cut(s) 489
HgaI GACGC 2 cut(s) 329, 854
HhaI GCGC 1 cut(s) 762
Hin1II CATG 7 cut(s) 221, 327, 538, 626, 630, 673, 862
Hin6I GCGC 1 cut(s) 760
HinP1I GCGC 1 cut(s) 760
HincII GTYRAC 1 cut(s) 877
HindII GTYRAC 1 cut(s) 877
HinfI GANTC 4 cut(s) 94, 619, 784, 878
HphI GGTGA 2 cut(s) 233, 827
Hpy166II GTNNAC 3 cut(s) 215, 573, 877
Hpy188I TCNGA 3 cut(s) 268, 340, 653
Hpy188III TCNNGA 5 cut(s) 70, 98, 296, 560, 851
Hpy8I GTNNAC 3 cut(s) 215, 573, 877
HpyAV CCTTC 1 cut(s) 44
HpyCH4III ACNGT 1 cut(s) 848
HpyCH4V TGCA 8 cut(s) 38, 89, 215, 230, 304, 626, 630, 749
HpyF10VI GCNNNNNNNGC 1 cut(s) 500
HpyF3I CTNAG 3 cut(s) 99, 137, 499
Hsp92II CATG 7 cut(s) 221, 327, 538, 626, 630, 673, 862
HspAI GCGC 1 cut(s) 760
Kzo9I GATC 2 cut(s) 190, 268
LmnI GCTCC 1 cut(s) 344
Lsp1109I GCAGC 2 cut(s) 466, 629
LweI GCATC 5 cut(s) 217, 313, 639, 682, 852
MalI GATC 2 cut(s) 192, 270
MboI GATC 2 cut(s) 190, 268
MboII GAAGA 4 cut(s) 236, 263, 389, 628
MhlI GDGCHC 2 cut(s) 217, 669
MluCI AATT 8 cut(s) 62, 73, 123, 470, 512, 563, 712, 821
MlyI GAGTC 1 cut(s) 872
MmeI TCCRAC 1 cut(s) 501
MnlI CCTC 5 cut(s) 367, 391, 394, 423, 693
Mph1103I ATGCAT 2 cut(s) 628, 632
MseI TTAA 2 cut(s) 549, 824
MslI CAYNNNNRTG 1 cut(s) 276
MspR9I CCNGG 2 cut(s) 195, 423
MvaI CCWGG 2 cut(s) 195, 423
MwoI GCNNNNNNNGC 1 cut(s) 500
NdeII GATC 2 cut(s) 190, 268
NlaIII CATG 7 cut(s) 221, 327, 538, 626, 630, 673, 862
NsiI ATGCAT 2 cut(s) 628, 632
NspI RCATGY 3 cut(s) 327, 538, 630
PaeI GCATGC 2 cut(s) 327, 630
PfeI GAWTC 3 cut(s) 94, 619, 784
PflFI GACNNNGTC 1 cut(s) 161
PfoI TCCNGGA 1 cut(s) 193
PkrI GCNGC 2 cut(s) 481, 644
PleI GAGTC 1 cut(s) 872
PpsI GAGTC 1 cut(s) 872
PpuMI RGGWCCY 1 cut(s) 754
Psp5II RGGWCCY 1 cut(s) 754
Psp6I CCWGG 2 cut(s) 193, 421
PspGI CCWGG 2 cut(s) 193, 421
PspPI GGNCC 1 cut(s) 754
PspPPI RGGWCCY 1 cut(s) 754
PsyI GACNNNGTC 1 cut(s) 161
RsaI GTAC 4 cut(s) 44, 235, 467, 572
RsaNI GTAC 4 cut(s) 43, 234, 466, 571
RseI CAYNNNNRTG 1 cut(s) 276
SaqAI TTAA 2 cut(s) 549, 824
SatI GCNGC 2 cut(s) 480, 643
Sau3AI GATC 2 cut(s) 190, 268
Sau96I GGNCC 1 cut(s) 754
SchI GAGTC 1 cut(s) 872
ScrFI CCNGG 2 cut(s) 195, 423
SduI GDGCHC 2 cut(s) 217, 669
SfaNI GCATC 5 cut(s) 217, 313, 639, 682, 852
SfcI CTRYAG 1 cut(s) 771
SinI GGWCC 1 cut(s) 754
SmiMI CAYNNNNRTG 1 cut(s) 276
SmlI CTYRAG 1 cut(s) 355
SmoI CTYRAG 1 cut(s) 355
SphI GCATGC 2 cut(s) 327, 630
Sse9I AATT 8 cut(s) 62, 73, 123, 470, 512, 563, 712, 821
SsiI CCGC 1 cut(s) 82
StyD4I CCNGG 2 cut(s) 193, 421
StyI CCWWGG 1 cut(s) 779
TaaI ACNGT 1 cut(s) 848
TaqI TCGA 2 cut(s) 130, 698
TasI AATT 8 cut(s) 62, 73, 123, 470, 512, 563, 712, 821
TatI WGTACW 3 cut(s) 233, 465, 570
TfiI GAWTC 3 cut(s) 94, 619, 784
Tru1I TTAA 2 cut(s) 549, 824
Tru9I TTAA 2 cut(s) 549, 824
TseI GCWGC 2 cut(s) 479, 642
TspDTI ATGAA 3 cut(s) 303, 525, 611
TspGWI ACGGA 1 cut(s) 733
Tth111I GACNNNGTC 1 cut(s) 161
VneI GTGCAC 1 cut(s) 213
VpaK11BI GGWCC 1 cut(s) 754
XapI RAATTY 4 cut(s) 73, 470, 563, 712
XceI RCATGY 3 cut(s) 327, 538, 630
Zsp2I ATGCAT 2 cut(s) 628, 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.