Rmu_sc0003363.1_g000069

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003363.1
OG0012492
Physical Location & Seq
Reverse (-)
356714 .. 357792
1079 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 966 bp
atggctaagaaaatcaacatagagagtgatgctcaagcaaacctccacacatcctcttcgtcctcctcctcctcctcctcaaccttgacaacccatgattctttgatcgataaaagcacagagaatgatcagaattcagacaaggtctcaagctcaaaagggcaagagctaaacaatgggagtaatgagcctaacaccaagaagaggcagaggagcagcagcactgatcatggcgatggaggcaggcacccgacttttcggggagtacgaatgcgctcttggggcaaatgggtgtccgaaatccgcgagccgaggaagaagtcaagaatttggttagggacatacccaacagctgaaatggcagcccgagcgcacgatgtggctgctcgggctatcaaaggtgcttctgcttatctaaatttccctgaattggctcaaacgcttccacaaccggcttctgcttctcccaaggacattcaagctgctgctgcaaaagctgcttctgcttttgaggagtacagcttgttcttcatcttccacctggctgctcgggctatcaaaggtgcttctgcttatctaaatttccctgaattggctcaaacgcttccacaaccggcttctgcttctcccaaggacattcaagctgctgctgcaaaagctgcttctgcttttgaggaggtgaagaaatgtgaagctgaaacggaagaagaagaagaagaggaagcaacactgcccagcaaagatgaacagacaactactactgctaatactagtatcagtacagcttgttcttcatcttccacttcaaatgattatgatgataagttgtttgaccttccagatcttttcatggatggcacaaatgggagtgatcaattttgcttttcttcttcatcatcatcatggcatctgtgtgcagcagatactggattcagactagaagagccattcttgtgggagtattaa

Protein Analysis

321

Amino Acids

34.81

Weight (kDa)

5.14

Isoelectric Point (pI)

54.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 85 - 134 4.8e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012492)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44940
fragaria_vesca FvH4_7g09550
malus_domestica MD02G1217900.v1.1 MD07G1099400.v1.1
prunus_persica Prupe.2G123200_v2.0.a1
pyrus_communis pycom07g08150 pycom1310g00030
rosa_chinensis RchiOBHm_Chr1g0346421
rosa_laevigata RLG00000007751 RLG00000028801
rosa_multiflora Rmu_sc0003363.1_g000069 Rmu_sc0022454.1_g000001
rosa_roxburghii Rroxscaffold_4G00308600
rosa_rugosa Rorug01G0179600
rosa_samantha Rh1AG199100 Rh1BG164800 Rh1CG184100 Rh1DG195600
rosa_wichuraiana Rw1G016700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 246
AccII CGCG 1 cut(s) 306
AciI CCGC 1 cut(s) 304
AcsI RAATTY 4 cut(s) 133, 327, 418, 580
AdeI CACNNNGTG 1 cut(s) 379
AfaI GTAC 3 cut(s) 267, 518, 781
AfiI CCNNNNNNNGG 3 cut(s) 204, 430, 592
AgsI TTSAA 3 cut(s) 479, 641, 807
AhlI ACTAGT 1 cut(s) 770
AjnI CCWGG 1 cut(s) 540
AjuI GAANNNNNNNTTGG 2 cut(s) 262, 294
Alw26I GTCTC 1 cut(s) 151
AlwNI CAGNNNCTG 1 cut(s) 926
Ama87I CYCGRG 3 cut(s) 366, 387, 549
ApoI RAATTY 4 cut(s) 133, 327, 418, 580
AspLEI GCGC 2 cut(s) 276, 373
AsuHPI GGTGA 1 cut(s) 691
AvaI CYCGRG 3 cut(s) 366, 387, 549
BanI GGYRCC 1 cut(s) 246
BccI CCATC 2 cut(s) 230, 848
BcgI CGANNNNNNTGC 2 cut(s) 365, 399
BciT130I CCWGG 1 cut(s) 542
BclI TGATCA 3 cut(s) 127, 226, 871
BcoDI GTCTC 1 cut(s) 151
BcuI ACTAGT 1 cut(s) 770
BfaI CTAG 2 cut(s) 771, 938
BglII AGATCT 1 cut(s) 841
Bme1390I CCNGG 1 cut(s) 542
BmeT110I CYCGRG 3 cut(s) 366, 387, 549
BmiI GGNNCC 1 cut(s) 248
BmrFI CCNGG 1 cut(s) 542
BmsI GCATC 2 cut(s) 19, 916
BplI GAGNNNNNCTC 2 cut(s) 16, 48
BpuEI CTTGAG 2 cut(s) 18, 133
Bsa29I ATCGAT 1 cut(s) 108
BsaI GGTCTC 1 cut(s) 151
BsaJI CCNNGG 3 cut(s) 311, 468, 630
Bsc4I CCNNNNNNNGG 3 cut(s) 204, 430, 592
Bse118I RCCGGY 2 cut(s) 451, 613
Bse1I ACTGG 1 cut(s) 931
BseBI CCWGG 1 cut(s) 542
BseCI ATCGAT 1 cut(s) 108
BseDI CCNNGG 3 cut(s) 311, 468, 630
BseGI GGATG 2 cut(s) 50, 859
BseLI CCNNNNNNNGG 3 cut(s) 204, 430, 592
BseNI ACTGG 1 cut(s) 931
BseRI GAGGAG 8 cut(s) 55, 58, 61, 64, 67, 226, 527, 689
BseYI CCCAGC 1 cut(s) 734
BsgI GTGCAG 1 cut(s) 936
Bsh1236I CGCG 1 cut(s) 306
BshNI GGYRCC 1 cut(s) 246
BshVI ATCGAT 1 cut(s) 108
BsiHKCI CYCGRG 3 cut(s) 366, 387, 549
BsiSI CCGG 2 cut(s) 452, 614
BslFI GGGAC 1 cut(s) 352
BslI CCNNNNNNNGG 3 cut(s) 204, 430, 592
BsmAI GTCTC 1 cut(s) 151
BsmFI GGGAC 1 cut(s) 352
BsmI GAATGC 1 cut(s) 276
Bso31I GGTCTC 1 cut(s) 151
BsoBI CYCGRG 3 cut(s) 366, 387, 549
Bsp143I GATC 5 cut(s) 105, 127, 226, 841, 871
BspACI CCGC 1 cut(s) 304
BspDI ATCGAT 1 cut(s) 108
BspFNI CGCG 1 cut(s) 306
BspLI GGNNCC 1 cut(s) 248
BspQI GCTCTTC 1 cut(s) 936
BspT107I GGYRCC 1 cut(s) 246
BspTNI GGTCTC 1 cut(s) 151
BsrFI RCCGGY 2 cut(s) 451, 613
BsrI ACTGG 1 cut(s) 931
BssAI RCCGGY 2 cut(s) 451, 613
BssECI CCNNGG 3 cut(s) 311, 468, 630
BssMI GATC 5 cut(s) 105, 127, 226, 841, 871
BssT1I CCWWGG 2 cut(s) 468, 630
Bst2UI CCWGG 1 cut(s) 542
Bst6I CTCTTC 4 cut(s) 61, 197, 711, 936
BstAPI GCANNNNNTGC 2 cut(s) 497, 659
BstC8I GCNNGC 2 cut(s) 245, 308
BstDEI CTNAG 1 cut(s) 6
BstF5I GGATG 2 cut(s) 50, 859
BstFNI CGCG 1 cut(s) 306
BstHHI GCGC 2 cut(s) 276, 373
BstKTI GATC 5 cut(s) 108, 130, 229, 844, 874
BstMAI GTCTC 1 cut(s) 151
BstMBI GATC 5 cut(s) 105, 127, 226, 841, 871
BstNI CCWGG 1 cut(s) 542
BstSCI CCNGG 1 cut(s) 540
BstUI CGCG 1 cut(s) 306
BstX2I RGATCY 1 cut(s) 841
BstXI CCANNNNNNTGG 1 cut(s) 954
BstYI RGATCY 1 cut(s) 841
Bsu15I ATCGAT 1 cut(s) 108
BsuTUI ATCGAT 1 cut(s) 108
BtgZI GCGATG 1 cut(s) 249
BtsCI GGATG 2 cut(s) 50, 859
BtsI GCAGTG 1 cut(s) 728
BtsIMutI CAGTG 2 cut(s) 222, 728
Cac8I GCNNGC 2 cut(s) 245, 308
CaiI CAGNNNCTG 1 cut(s) 926
CfoI GCGC 2 cut(s) 276, 373
Cfr10I RCCGGY 2 cut(s) 451, 613
ClaI ATCGAT 1 cut(s) 108
Csp6I GTAC 3 cut(s) 266, 517, 780
CviAII CATG 4 cut(s) 95, 230, 850, 903
CviQI GTAC 3 cut(s) 266, 517, 780
DdeI CTNAG 1 cut(s) 6
DpnI GATC 5 cut(s) 107, 129, 228, 843, 873
DpnII GATC 5 cut(s) 105, 127, 226, 841, 871
DraIII CACNNNGTG 1 cut(s) 379
Eam1104I CTCTTC 4 cut(s) 61, 197, 711, 936
EarI CTCTTC 4 cut(s) 61, 197, 711, 936
Eco130I CCWWGG 2 cut(s) 468, 630
Eco31I GGTCTC 1 cut(s) 151
Eco88I CYCGRG 3 cut(s) 366, 387, 549
EcoRI GAATTC 1 cut(s) 133
EcoRII CCWGG 1 cut(s) 540
EcoT14I CCWWGG 2 cut(s) 468, 630
ErhI CCWWGG 2 cut(s) 468, 630
FaeI CATG 4 cut(s) 98, 233, 853, 906
FaiI YATR 7 cut(s) 20, 96, 231, 343, 816, 851, 904
FaqI GGGAC 1 cut(s) 352
FatI CATG 4 cut(s) 94, 229, 849, 902
FbaI TGATCA 3 cut(s) 127, 226, 871
FokI GGATG 2 cut(s) 37, 866
FspBI CTAG 2 cut(s) 771, 938
GlaI GCGC 2 cut(s) 275, 372
GsaI CCCAGC 1 cut(s) 738
HapII CCGG 2 cut(s) 452, 614
HhaI GCGC 2 cut(s) 276, 373
Hin1II CATG 4 cut(s) 98, 233, 853, 906
Hin6I GCGC 2 cut(s) 274, 371
HinP1I GCGC 2 cut(s) 274, 371
HinfI GANTC 2 cut(s) 98, 930
HpaII CCGG 2 cut(s) 452, 614
HphI GGTGA 1 cut(s) 691
Hpy188I TCNGA 4 cut(s) 132, 139, 298, 935
Hpy188III TCNNGA 2 cut(s) 324, 839
HpyAV CCTTC 1 cut(s) 845
HpyCH4V TGCA 3 cut(s) 491, 653, 917
HpyF3I CTNAG 1 cut(s) 6
Hsp92II CATG 4 cut(s) 98, 233, 853, 906
HspAI GCGC 2 cut(s) 274, 371
Ksp22I TGATCA 3 cut(s) 127, 226, 871
Kzo9I GATC 5 cut(s) 105, 127, 226, 841, 871
LguI GCTCTTC 1 cut(s) 936
LmnI GCTCC 1 cut(s) 213
LweI GCATC 2 cut(s) 19, 916
MaeI CTAG 2 cut(s) 771, 938
MalI GATC 5 cut(s) 107, 129, 228, 843, 873
MboI GATC 5 cut(s) 105, 127, 226, 841, 871
MflI RGATCY 1 cut(s) 841
MluCI AATT 7 cut(s) 133, 327, 418, 428, 580, 590, 875
MseI TTAA 1 cut(s) 964
MslI CAYNNNNRTG 4 cut(s) 234, 901, 912, 952
MspA1I CMGCKG 1 cut(s) 353
MspI CCGG 2 cut(s) 452, 614
MspR9I CCNGG 1 cut(s) 542
Mva1269I GAATGC 1 cut(s) 276
MvaI CCWGG 1 cut(s) 542
MvnI CGCG 1 cut(s) 306
NdeII GATC 5 cut(s) 105, 127, 226, 841, 871
NlaIII CATG 4 cut(s) 98, 233, 853, 906
NlaIV GGNNCC 1 cut(s) 248
NmeAIII GCCGAG 1 cut(s) 336
PciSI GCTCTTC 1 cut(s) 936
PcsI WCGNNNNNNNCGW 1 cut(s) 265
PctI GAATGC 1 cut(s) 276
PfeI GAWTC 2 cut(s) 98, 930
PflFI GACNNNGTC 1 cut(s) 143
Psp6I CCWGG 1 cut(s) 540
PspFI CCCAGC 1 cut(s) 734
PspGI CCWGG 1 cut(s) 540
PspN4I GGNNCC 1 cut(s) 248
PsrI GAACNNNNNNTAC 4 cut(s) 509, 541, 772, 804
PstNI CAGNNNCTG 1 cut(s) 926
PsuI RGATCY 1 cut(s) 841
PsyI GACNNNGTC 1 cut(s) 143
PvuII CAGCTG 1 cut(s) 353
RsaI GTAC 3 cut(s) 267, 518, 781
RsaNI GTAC 3 cut(s) 266, 517, 780
RseI CAYNNNNRTG 4 cut(s) 234, 901, 912, 952
SapI GCTCTTC 1 cut(s) 936
SaqAI TTAA 1 cut(s) 964
Sau3AI GATC 5 cut(s) 105, 127, 226, 841, 871
ScrFI CCNGG 1 cut(s) 542
SfaNI GCATC 2 cut(s) 19, 916
SmiMI CAYNNNNRTG 4 cut(s) 234, 901, 912, 952
SmlI CTYRAG 2 cut(s) 33, 148
SmoI CTYRAG 2 cut(s) 33, 148
SpeI ACTAGT 1 cut(s) 770
Sse9I AATT 7 cut(s) 133, 327, 418, 428, 580, 590, 875
SsiI CCGC 1 cut(s) 304
SspMI CTAG 2 cut(s) 771, 938
StyD4I CCNGG 1 cut(s) 540
StyI CCWWGG 2 cut(s) 468, 630
TaqI TCGA 1 cut(s) 108
TasI AATT 7 cut(s) 133, 327, 418, 428, 580, 590, 875
TatI WGTACW 2 cut(s) 516, 779
TfiI GAWTC 2 cut(s) 98, 930
Tru1I TTAA 1 cut(s) 964
Tru9I TTAA 1 cut(s) 964
TscAI CASTG 2 cut(s) 229, 735
TspDTI ATGAA 5 cut(s) 520, 759, 783, 838, 882
TspGWI ACGGA 1 cut(s) 716
TspRI CASTG 2 cut(s) 229, 735
Tth111I GACNNNGTC 1 cut(s) 143
XapI RAATTY 4 cut(s) 133, 327, 418, 580
XspI CTAG 2 cut(s) 771, 938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.