Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0003890.1

Orthogroup ID

OG0002992

Physical Location & Seq

Genomic Coordinates Reverse (-)

1 .. 10943

Gene Structure 10943 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4427 bp

atggaagatgaattgtataatgaagaactagtgccatgtgcttgcataactggggtacatctaaatgtttccactcgggaagatacggagaaaatatcaacattattaatcgaggcagctgcccaggttaatgatccaaagttgggacttccaaatccaacgaatcagtgttctacttgtggtacccaagatataaaatcctgtgatggtcattttgggtccatcaagtttccgtgcacgatactccatccatactatctaccggaagttgctcttattttaaacaagatttgcccggcatgtaaaaccatcaggcaaggtgtaagggtcaagggatctgaacggaggggctgcagatattgctttggaggttcatcggattaccctagaatgaagttcaaagtttcttctagagagcttttcagaagaagtacaataatagtagaagcagttggaaaattgcctcctgattattgggacttcattcccaaagatccccaacatgatgaaacacacacaaaaccaaatagaagagttctatctcatgcccaggttcattttttgttgaatgatgttgatccagatttcatcacaaagtttgttccaacaaccagctcactttctttggactgtttccttgtcacaccaaattgtcatcgtgtgacagaagttatgtattcatttaataatgggcagtcattgatgtttgatgatcggactagagcttacaggaaactagtggatttcagaggcaccgcaaacgagctgggttctcgtgttttggactgcctgaaaatttccaagataaacaaagacaaaacgggcagagattcaatttcagttgagcaacaaaagattaaggattctccgtccagaacatccggcttgagatggataaaagatgttgttcttggaaagagaagtgatcactgttttcgaactgttgtcgttggtgatccaaacatcaagctgagtgagattggcataccccgccatattgcagagaaaatgcagatctctgagaatctgaaccaatataaccttgagaagttgtatgcgttttgccagctacgccttggccatcatgggagaggtgacatacatgttcgtagaaatgatagtctggttaggataagtaatttagaagaactggagatgggagatattatatacagggcgttgagtgatggagatgttgtgttgataaataggcctccatcaatacatcaacactccctaatagctttaactgcaaagatccttccagtaaactctgtagtgtcgataaatccactgtgttgctctccttttcgtggtgattttgatggtgattgtcttcatggttatgttcctcaatcagtggataccagagtcgagcttacagagcttgttgctttagataagcaattagttaatgggcagagtggttccaaccttctttctctcagtcaagatagtttaactgcttgctatctcatcatggaagatggcaccctgatgaataagtttcaaatgcaacaactgaaaatgctttgtccccctgaactgccatcaccagcgataatcaaagatccttctggtaactccgtttcttggacggcaaagcagataatcagcatgtttctacctgcaaattttaattttgcatttccttcaaatggcgtgcatattagcaatggagagcttctagcttcttctgaaggttcttcttggctgcgagacaccagtgggaatcttttccaaagtctcatcgaacattgccccggtcagatactggacatcttctttactgctcagggagttctatgtgagtggttgtcaatgaggggcttgagtgtttctctctcagacttgtatatcgcgtcggatacatgttcaaggaaaaacttggtggaggaaatcttctatgggttacaagaagcagaggaggcatgtactatcagacagttgatgcttgattcttgtcaggaatttcttatgggctatgctgaagagacaaaaagccctttgacttttggttcagaacatttttgttatcagaagcagaaatctgctgcacttagtcaggtcacggtagatgctttcaaagaaggtttccgggatgtccaaaatttagcatacaaatatgcccgtgaggacaactcgttgctggctatgtttaaggctggaagcaagggtaatatgctgaaattagttcagcatggtatgtgccttggtttgcagcattcactagttcccttatcattcaagtttccgaaccagctctcatgtgatgcatggaatgatcaaaaagcacgtggtatcgttcaggaggttggaagagcttttgaatccatcgagtcatatatcccatccgctgtcgttaaaagttcgttcttgactgggctgaatccggtcgaatgctttgttcattcagtcacgagtcgagatggttcttttagtaataatgcggaacttcctgggactctgaatcgaaagctcatgtatttcatgcgtgatttgcatactgcttatgacggaacagttagaaacacatatgggaaccagctggttcagttctcctatgataatgacgagggcatatctactgcagatggcaccacaaacagctcatatgcaaagagtgttattgattgtggtggcggccaacccgttggtgcattgtctgcttgtgcaatatctgaagctgcatacagtgctttagatcagaaggttcttcctctctcctcactcttctccctgcatcctcttctctctgcaaaagttattttattaatttttttcttgttctttcagaagaacacctgtcttacttatgtgcgatgctctttaattgcacagaacattttggagtgtggttcaaagaaaaggagtgctaagcaaactatgtcattatttttatctgagaagcttggaagacctaggcatggtttcgagtatggagcgctagaagttaaaaatcatctagagggactcacaatcaggttctcctcacaaactggcagcaagatgcattttagtccttgggtttctcactttcatatatctaaggaaattgtacgaagaagaaggctgaatgtgcgatctattatagaggcactttatgcaagatgcaactccgctccaatggagttgaaaagtatcctttggaatttgaagatcacaagaaataaggaatgtgattgctgcagcacacctaatgatacattttgcatcagtgtcgcactgattgaaaagcaagacaattcttctgaagaactggataaaaagcaagacaagtcttctgaagaactggataaaactcgagtcttggtgataccattcttgcttaaaacagtagttaaaggttttctggagataaaaaaggtggatattatatggaatgatcgaactggttcacctggtgaactctacttgagagtttctatgtcaggaaagaccagaaggggggctaccttttggaatatgcttatggacgattgtcttccggttatggatatgattgattggtcacgaagtcatccagataacgtcgatgacttctgcactgcctatggaatagatgttgggtggaaacatttccttaataagttggagtctgcaaccgtagatattggaaagacaattcttcctcagcatttgcttcttgcagcagattgtctgtcagccaccggcgagtttgtcagcttaaacgcaaaaggcattgctcaacaattggaacatgcatctgtcacagccccttttatgcaagcttgcttttctactcctggtccttgcttggttaaagctgctaaggctggagtggtggataatctccaggggagtttggacgcattgacatggggaaatactccgtccttgggaagtggtggacagtttgatattatatttgctggggagggatctgtgtcttctaagccatttgatgttcatgatatgctgagcagacaaatcagttccaacccccagaatgtatttgaagtgtctgatgctccaattcatctatctgacaaatatgttgctccacatgtttacaagtatggtcttttgtgcaagttgaagagtattaccaaatcagtcttaagagaaacttttacgatccaggatattgatagattgtctcaaaccttgaagaatatattgcacaagtatgacatcaatgaaagcataaatgagatggagaagaaagatttgatgacggctttagaatttcaccctcatagggatcgtaagatcggattaggaatcaaagatataaaggttggccagcaccctaaccatgagaatgcacgatgcttcatgctagtacatacggatgggacgattgaagatgtttcgtaccacaagtgcatcattggtgcttttgatatcatagctcctcatagggcaaagggctatcgcctgagacatttgagccgtggtcctcg

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000418 GO:0000428 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010495 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901698 GO:1901699 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3223 RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

1476

Amino Acids

164.87

Weight (kDa)

7.11

Isoelectric Point (pI)

42.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	43 - 104	4.5e-07	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	305 - 464	1.9e-35	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	469 - 618	3.4e-19	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	687 - 742	3.8e-08	RNA polymerase Rpb1, domain 4
DUF3223	PF11523	1378 - 1451	5.2e-25	Protein of unknown function (DUF3223)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002992)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63020 AT1G63020 AT1G63020 AT1G63020 AT1G63020
fragaria_vesca	FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400
malus_domestica	MD04G1068800.v1.1 MD06G1069300.v1.1 MD06G1069400.v1.1 MD06G1069500.v1.1 MD06G1069800.v1.1
prunus_persica	Prupe.5G089300_v2.0.a1
pyrus_communis	pycom04g06250
rosa_chinensis	RchiOBHm_Chr7g0199481
rosa_laevigata	RLG00000003790
rosa_multiflora	Rmu_co8411067.1_g000001 Rmu_sc0003890.1_g000001
rosa_roxburghii	Rroxscaffold_3G00256700
rosa_rugosa	Rorug07G0054300 Rorug07G0054400 Rorug07G0054500 Rorug07G0054500
rosa_samantha	Rh7AG179300 Rh7BG182800 Rh7CG188100 Rh7DG182200
rosa_wichuraiana	Rw7G015760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1636
Acc65I	GGTACC	1 cut(s)	182
AccB1I	GGYRCC	4 cut(s)	182, 752, 1490, 2597
AccBSI	CCGCTC	1 cut(s)	3122
AccII	CGCG	1 cut(s)	1862
AciI	CCGC	6 cut(s)	756, 991, 2355, 2450, 2643, 3120
AcoI	YGGCCR	3 cut(s)	1078, 2644, 4264
AcsI	RAATTY	6 cut(s)	795, 1633, 1970, 2110, 3151, 4208
AcuI	CTGAAG	5 cut(s)	1719, 2010, 2703, 3273, 3306
AcvI	CACGTG	1 cut(s)	2297
AdeI	CACNNNGTG	1 cut(s)	3404
AfaI	GTAC	7 cut(s)	57, 184, 433, 1936, 3060, 4308, 4340
AfeI	AGCGCT	1 cut(s)	2946
AfiI	CCNNNNNNNGG	8 cut(s)	143, 1313, 1593, 1658, 2927, 3447, 3860, 4222
AflII	CTTAAG	1 cut(s)	4081
AflIII	ACRYGT	3 cut(s)	1102, 1871, 4027
AhlI	ACTAGT	3 cut(s)	28, 736, 2230
AjnI	CCWGG	7 cut(s)	123, 549, 2458, 3400, 3766, 3816, 4101
Alw21I	GWGCWC	1 cut(s)	239
Alw26I	GTCTC	5 cut(s)	1713, 1751, 1988, 4125, 4399
Alw44I	GTGCAC	1 cut(s)	235
AlwNI	CAGNNNCTG	1 cut(s)	1774
Ama87I	CYCGRG	2 cut(s)	75, 3303
Aor51HI	AGCGCT	1 cut(s)	2946
AoxI	GGCC	4 cut(s)	1078, 1211, 2644, 4264
ApaLI	GTGCAC	1 cut(s)	235
ApoI	RAATTY	6 cut(s)	795, 1633, 1970, 2110, 3151, 4208
AseI	ATTAAT	2 cut(s)	107, 2774
Asp700I	GAANNNNTTC	3 cut(s)	1736, 3394, 3578
Asp718I	GGTACC	1 cut(s)	182
AspA2I	CCTAGG	1 cut(s)	2921
AspLEI	GCGC	1 cut(s)	2947
AspS9I	GGNCC	3 cut(s)	219, 3770, 4421
AsuC2I	CCSGG	3 cut(s)	296, 1764, 2099
AsuHPI	GGTGA	9 cut(s)	965, 1106, 1328, 1340, 1545, 3325, 3390, 3416, 4205
AsuII	TTCGAA	1 cut(s)	937
AvaI	CYCGRG	2 cut(s)	75, 3303
AvaII	GGWCC	3 cut(s)	219, 3770, 4421
AvrII	CCTAGG	1 cut(s)	2921
BaeGI	GKGCMC	1 cut(s)	239
BaeI	ACNNNNGTAYC	2 cut(s)	1356, 1389
BalI	TGGCCA	2 cut(s)	1080, 4266
BanI	GGYRCC	4 cut(s)	182, 752, 1490, 2597
BarI	GAAGNNNNNNTAC	2 cut(s)	3436, 3468
BauI	CACGAG	2 cut(s)	774, 2419
BbrPI	CACGTG	1 cut(s)	2297
BbsI	GAAGAC	5 cut(s)	1328, 2923, 3273, 3476, 3903
Bbv12I	GWGCWC	1 cut(s)	239
BbvCI	CCTCAGC	1 cut(s)	3633
BceAI	ACGGC	3 cut(s)	1614, 4215, 4401
BcgI	CGANNNNNNTGC	4 cut(s)	101, 135, 3202, 3236
BciT130I	CCWGG	7 cut(s)	125, 551, 2460, 3402, 3768, 3818, 4103
BciVI	GTATCC	3 cut(s)	1357, 1861, 3152
BclI	TGATCA	2 cut(s)	925, 2284
BcnI	CCSGG	3 cut(s)	296, 1764, 2099
BcoDI	GTCTC	5 cut(s)	1713, 1751, 1988, 4125, 4399
BcuI	ACTAGT	3 cut(s)	28, 736, 2230
BfmI	CTRYAG	4 cut(s)	352, 1275, 2589, 3187
BfoI	RGCGCY	1 cut(s)	2948
BfrI	CTTAAG	1 cut(s)	4081
BfuAI	ACCTGC	1 cut(s)	1636
BfuI	GTATCC	3 cut(s)	1357, 1861, 3152
BglII	AGATCT	1 cut(s)	1014
BlnI	CCTAGG	1 cut(s)	2921
BlpI	GCTNAGC	2 cut(s)	2877, 3941
Bme18I	GGWCC	3 cut(s)	219, 3770, 4421
BmeT110I	CYCGRG	2 cut(s)	75, 3303
BmgT120I	GGNCC	3 cut(s)	219, 3770, 4421
BmiI	GGNNCC	7 cut(s)	184, 220, 754, 1429, 1492, 2543, 2599
BmrI	ACTGGG	2 cut(s)	60, 2391
BmuI	ACTGGG	2 cut(s)	60, 2391
BoxI	GACNNNNGTC	1 cut(s)	3480
BpiI	GAAGAC	5 cut(s)	1328, 2923, 3273, 3476, 3903
BplI	GAGNNNNNCTC	4 cut(s)	1825, 1857, 2126, 2158
BpmI	CTGGAG	4 cut(s)	1172, 3374, 3800, 3819
Bpu10I	CCTNAGC	3 cut(s)	1794, 3633, 3792
Bpu1102I	GCTNAGC	2 cut(s)	2877, 3941
Bpu14I	TTCGAA	1 cut(s)	937
BpuEI	CTTGAG	4 cut(s)	907, 1064, 1852, 3436
BpuMI	CCSGG	3 cut(s)	296, 1764, 2099
BsaAI	YACGTR	1 cut(s)	2297
BsaBI	GATNNNNATC	1 cut(s)	576
BsaWI	WCCGGW	3 cut(s)	262, 2392, 3487
BsaXI	ACNNNNNCTCC	2 cut(s)	1182, 1212
Bsc4I	CCNNNNNNNGG	8 cut(s)	143, 1313, 1593, 1658, 2927, 3447, 3860, 4222
Bse118I	RCCGGY	1 cut(s)	3671
Bse3DI	GCAATG	3 cut(s)	1681, 1756, 3702
Bse8I	GATNNNNATC	1 cut(s)	576
BseBI	CCWGG	7 cut(s)	125, 551, 2460, 3402, 3768, 3818, 4103
BseGI	GGATG	7 cut(s)	247, 878, 2107, 2351, 2743, 3520, 4321
BseJI	GATNNNNATC	1 cut(s)	576
BseLI	CCNNNNNNNGG	8 cut(s)	143, 1313, 1593, 1658, 2927, 3447, 3860, 4222
BseMI	GCAATG	3 cut(s)	1681, 1756, 3702
BseMII	CTCAG	9 cut(s)	962, 1011, 1459, 1808, 1860, 2895, 3647, 3932, 4394
BseRI	GAGGAG	4 cut(s)	1940, 2716, 2980, 4368
BseSI	GKGCMC	1 cut(s)	239
BseYI	CCCAGC	2 cut(s)	766, 3893
BsgI	GTGCAG	2 cut(s)	2040, 3529
Bsh1236I	CGCG	1 cut(s)	1862
Bsh1285I	CGRYCG	1 cut(s)	2397
BshFI	GGCC	4 cut(s)	1080, 1213, 2646, 4266
BshNI	GGYRCC	4 cut(s)	182, 752, 1490, 2597
BsiEI	CGRYCG	1 cut(s)	2397
BsiHKAI	GWGCWC	1 cut(s)	239
BsiHKCI	CYCGRG	2 cut(s)	75, 3303
BsiSI	CCGG	8 cut(s)	263, 296, 882, 1764, 2098, 2393, 3488, 3672
BslFI	GGGAC	6 cut(s)	159, 491, 1521, 2476, 2985, 4333
BslI	CCNNNNNNNGG	8 cut(s)	143, 1313, 1593, 1658, 2927, 3447, 3860, 4222
BsmAI	GTCTC	5 cut(s)	1713, 1751, 1988, 4125, 4399
BsmFI	GGGAC	6 cut(s)	159, 491, 1521, 2476, 2985, 4333
BsmI	GAATGC	3 cut(s)	2224, 2405, 4291
BsnI	GGCC	4 cut(s)	1080, 1213, 2646, 4266
BsoBI	CYCGRG	2 cut(s)	75, 3303
Bsp119I	TTCGAA	1 cut(s)	937
Bsp1286I	GDGCHC	1 cut(s)	239
Bsp1720I	GCTNAGC	2 cut(s)	2877, 3941
BspACI	CCGC	6 cut(s)	756, 991, 2355, 2450, 2643, 3120
BspANI	GGCC	4 cut(s)	1080, 1213, 2646, 4266
BspCNI	CTCAG	9 cut(s)	963, 1012, 1458, 1807, 1859, 2896, 3646, 3933, 4395
BspFNI	CGCG	1 cut(s)	1862
BspHI	TCATGA	1 cut(s)	3931
BspLI	GGNNCC	7 cut(s)	184, 220, 754, 1429, 1492, 2543, 2599
BspMAI	CTGCAG	3 cut(s)	356, 2593, 3191
BspMI	ACCTGC	1 cut(s)	1636
BspQI	GCTCTTC	1 cut(s)	2314
BspT104I	TTCGAA	1 cut(s)	937
BspT107I	GGYRCC	4 cut(s)	182, 752, 1490, 2597
BspTI	CTTAAG	1 cut(s)	4081
BsrBI	CCGCTC	1 cut(s)	3122
BsrDI	GCAATG	3 cut(s)	1681, 1756, 3702
BsrFI	RCCGGY	1 cut(s)	3671
BssAI	RCCGGY	1 cut(s)	3671
BssSI	CACGAG	2 cut(s)	774, 2419
BssT1I	CCWWGG	5 cut(s)	1075, 2212, 2921, 3023, 3858
Bst2BI	CACGAG	2 cut(s)	774, 2419
Bst2UI	CCWGG	7 cut(s)	125, 551, 2460, 3402, 3768, 3818, 4103
Bst6I	CTCTTC	6 cut(s)	526, 1986, 2314, 2738, 2754, 4055
BstAFI	CTTAAG	1 cut(s)	4081
BstAPI	GCANNNNNTGC	4 cut(s)	360, 2666, 2696, 3104
BstBAI	YACGTR	1 cut(s)	2297
BstBI	TTCGAA	1 cut(s)	937
BstC8I	GCNNGC	8 cut(s)	43, 1067, 1468, 1664, 2151, 3750, 3754, 4268
BstDSI	CCRYGG	1 cut(s)	4417
BstF5I	GGATG	7 cut(s)	247, 878, 2107, 2351, 2743, 3520, 4321
BstFNI	CGCG	1 cut(s)	1862
BstH2I	RGCGCY	1 cut(s)	2948
BstHHI	GCGC	1 cut(s)	2947
BstMAI	GTCTC	5 cut(s)	1713, 1751, 1988, 4125, 4399
BstMCI	CGRYCG	1 cut(s)	2397
BstNI	CCWGG	7 cut(s)	125, 551, 2460, 3402, 3768, 3818, 4103
BstNSI	RCATGY	7 cut(s)	303, 1106, 1621, 1875, 1935, 3725, 4031
BstPAI	GACNNNNGTC	1 cut(s)	3480
BstSFI	CTRYAG	4 cut(s)	352, 1275, 2589, 3187
BstSLI	GKGCMC	1 cut(s)	239
BstUI	CGCG	1 cut(s)	1862
BstV2I	GAAGAC	5 cut(s)	1328, 2923, 3273, 3476, 3903
BstX2I	RGATCY	6 cut(s)	335, 493, 1014, 1257, 1570, 3902
BstXI	CCANNNNNNTGG	1 cut(s)	2654
BstYI	RGATCY	6 cut(s)	335, 493, 1014, 1257, 1570, 3902
BsuI	GTATCC	3 cut(s)	1357, 1861, 3152
BsuRI	GGCC	4 cut(s)	1080, 1213, 2646, 4266
BtgI	CCRYGG	1 cut(s)	4417
BtgZI	GCGATG	1 cut(s)	2836
BtsCI	GGATG	7 cut(s)	247, 878, 2107, 2351, 2743, 3520, 4321
BtsI	GCAGTG	1 cut(s)	3546
BtsIMutI	CAGTG	9 cut(s)	173, 928, 1292, 1365, 1732, 2701, 3223, 3224, 3546
BveI	ACCTGC	1 cut(s)	1636
Cac8I	GCNNGC	8 cut(s)	43, 1067, 1468, 1664, 2151, 3750, 3754, 4268
CaiI	CAGNNNCTG	1 cut(s)	1774
CciI	TCATGA	1 cut(s)	3931
CfoI	GCGC	1 cut(s)	2947
Cfr10I	RCCGGY	1 cut(s)	3671
Cfr13I	GGNCC	3 cut(s)	219, 3770, 4421
CseI	GACGC	2 cut(s)	1851, 3839
CsiI	ACCWGGT	1 cut(s)	3400
Csp6I	GTAC	7 cut(s)	56, 183, 432, 1935, 3059, 4307, 4339
CviQI	GTAC	7 cut(s)	56, 183, 432, 1935, 3059, 4307, 4339
DraI	TTTAAA	1 cut(s)	282
DraIII	CACNNNGTG	1 cut(s)	3404
EaeI	YGGCCR	3 cut(s)	1078, 2644, 4264
Eam1104I	CTCTTC	6 cut(s)	526, 1986, 2314, 2738, 2754, 4055
EarI	CTCTTC	6 cut(s)	526, 1986, 2314, 2738, 2754, 4055
Eco130I	CCWWGG	5 cut(s)	1075, 2212, 2921, 3023, 3858
Eco147I	AGGCCT	1 cut(s)	1213
Eco32I	GATATC	1 cut(s)	4369
Eco47I	GGWCC	3 cut(s)	219, 3770, 4421
Eco47III	AGCGCT	1 cut(s)	2946
Eco57I	CTGAAG	5 cut(s)	1719, 2010, 2703, 3273, 3306
Eco72I	CACGTG	1 cut(s)	2297
Eco88I	CYCGRG	2 cut(s)	75, 3303
EcoRII	CCWGG	7 cut(s)	123, 549, 2458, 3400, 3766, 3816, 4101
EcoRV	GATATC	1 cut(s)	4369
EcoT14I	CCWWGG	5 cut(s)	1075, 2212, 2921, 3023, 3858
EcoT22I	ATGCAT	3 cut(s)	2278, 3015, 3727
ErhI	CCWWGG	5 cut(s)	1075, 2212, 2921, 3023, 3858
FalI	AAGNNNNNCTT	4 cut(s)	258, 290, 4075, 4107
FaqI	GGGAC	6 cut(s)	159, 491, 1521, 2476, 2985, 4333
FauI	CCCGC	1 cut(s)	998
FauNDI	CATATG	2 cut(s)	2536, 2614
FbaI	TGATCA	2 cut(s)	925, 2284
FokI	GGATG	7 cut(s)	234, 865, 2114, 2338, 2730, 3507, 4328
GlaI	GCGC	1 cut(s)	2946
GsaI	CCCAGC	2 cut(s)	770, 3897
GsuI	CTGGAG	4 cut(s)	1172, 3374, 3800, 3819
HaeII	RGCGCY	1 cut(s)	2948
HaeIII	GGCC	4 cut(s)	1080, 1213, 2646, 4266
HapII	CCGG	8 cut(s)	263, 296, 882, 1764, 2098, 2393, 3488, 3672
HgaI	GACGC	2 cut(s)	1851, 3839
HhaI	GCGC	1 cut(s)	2947
Hin6I	GCGC	1 cut(s)	2945
HinP1I	GCGC	1 cut(s)	2945
HindIII	AAGCTT	2 cut(s)	2909, 3750
HpaII	CCGG	8 cut(s)	263, 296, 882, 1764, 2098, 2393, 3488, 3672
HphI	GGTGA	9 cut(s)	965, 1106, 1328, 1340, 1545, 3325, 3390, 3416, 4205
Hpy166II	GTNNAC	6 cut(s)	237, 1270, 3398, 3407, 3872, 4033
Hpy8I	GTNNAC	6 cut(s)	237, 1270, 3398, 3407, 3872, 4033
Hpy99I	CGWCG	2 cut(s)	1867, 3536
HpyAV	CCTTC	9 cut(s)	1271, 1445, 1584, 1662, 1694, 2084, 2704, 3063, 3438
HpyCH4IV	ACGT	2 cut(s)	2296, 3531
HpySE526I	ACGT	2 cut(s)	2296, 3531
HspAI	GCGC	1 cut(s)	2945
KpnI	GGTACC	1 cut(s)	186
Ksp22I	TGATCA	2 cut(s)	925, 2284
LguI	GCTCTTC	1 cut(s)	2314
LmnI	GCTCC	5 cut(s)	2942, 3127, 3997, 4027, 4381
MabI	ACCWGGT	1 cut(s)	3400
MaeII	ACGT	2 cut(s)	2296, 3531
MaeIII	GTNAC	9 cut(s)	640, 661, 1094, 1580, 1911, 2068, 2416, 3510, 3730
MbiI	CCGCTC	1 cut(s)	3122
MfeI	CAATTG	1 cut(s)	3713
MflI	RGATCY	6 cut(s)	335, 493, 1014, 1257, 1570, 3902
MhlI	GDGCHC	1 cut(s)	239
MlsI	TGGCCA	2 cut(s)	1080, 4266
MluNI	TGGCCA	2 cut(s)	1080, 4266
MlyI	GAGTC	7 cut(s)	1380, 2348, 2431, 2458, 2967, 3315, 3605
MmeI	TCCRAC	8 cut(s)	182, 433, 629, 1455, 1845, 2296, 3573, 3984
Mox20I	TGGCCA	2 cut(s)	1080, 4266
Mph1103I	ATGCAT	3 cut(s)	2278, 3015, 3727
MroXI	GAANNNNTTC	3 cut(s)	1736, 3394, 3578
MscI	TGGCCA	2 cut(s)	1080, 4266
MslI	CAYNNNNRTG	7 cut(s)	63, 1344, 1496, 3838, 4149, 4284, 4314
Msp20I	TGGCCA	2 cut(s)	1080, 4266
MspA1I	CMGCKG	3 cut(s)	119, 2357, 2548
MspCI	CTTAAG	1 cut(s)	4081
MspI	CCGG	8 cut(s)	263, 296, 882, 1764, 2098, 2393, 3488, 3672
MunI	CAATTG	1 cut(s)	3713
Mva1269I	GAATGC	3 cut(s)	2224, 2405, 4291
MvaI	CCWGG	7 cut(s)	125, 551, 2460, 3402, 3768, 3818, 4103
MvnI	CGCG	1 cut(s)	1862
NciI	CCSGG	3 cut(s)	296, 1764, 2099
NdeI	CATATG	2 cut(s)	2536, 2614
NlaIV	GGNNCC	7 cut(s)	184, 220, 754, 1429, 1492, 2543, 2599
NmuCI	GTSAC	7 cut(s)	640, 661, 1094, 2068, 2416, 3510, 3730
NsiI	ATGCAT	3 cut(s)	2278, 3015, 3727
NspI	RCATGY	7 cut(s)	303, 1106, 1621, 1875, 1935, 3725, 4031
NspV	TTCGAA	1 cut(s)	937
PaeR7I	CTCGAG	1 cut(s)	3303
PagI	TCATGA	1 cut(s)	3931
PceI	AGGCCT	1 cut(s)	1213
PciI	ACATGT	3 cut(s)	1102, 1871, 4027
PciSI	GCTCTTC	1 cut(s)	2314
PcsI	WCGNNNNNNNCGW	1 cut(s)	4319
PctI	GAATGC	3 cut(s)	2224, 2405, 4291
PdmI	GAANNNNTTC	3 cut(s)	1736, 3394, 3578
PfoI	TCCNGGA	2 cut(s)	2097, 4101
PleI	GAGTC	7 cut(s)	1379, 2347, 2430, 2458, 2967, 3314, 3604
PmaCI	CACGTG	1 cut(s)	2297
PmlI	CACGTG	1 cut(s)	2297
PpsI	GAGTC	7 cut(s)	1379, 2347, 2430, 2458, 2967, 3314, 3604
Ppu21I	YACGTR	1 cut(s)	2297
PscI	ACATGT	3 cut(s)	1102, 1871, 4027
PshAI	GACNNNNGTC	1 cut(s)	3480
PshBI	ATTAAT	2 cut(s)	107, 2774
Psp6I	CCWGG	7 cut(s)	123, 549, 2458, 3400, 3766, 3816, 4101
PspCI	CACGTG	1 cut(s)	2297
PspFI	CCCAGC	2 cut(s)	766, 3893
PspGI	CCWGG	7 cut(s)	123, 549, 2458, 3400, 3766, 3816, 4101
PspN4I	GGNNCC	7 cut(s)	184, 220, 754, 1429, 1492, 2543, 2599
PspPI	GGNCC	3 cut(s)	219, 3770, 4421
PspXI	VCTCGAGB	1 cut(s)	3303
PstI	CTGCAG	3 cut(s)	356, 2593, 3191
PstNI	CAGNNNCTG	1 cut(s)	1774
PsuI	RGATCY	6 cut(s)	335, 493, 1014, 1257, 1570, 3902
PvuII	CAGCTG	2 cut(s)	119, 2548
RsaI	GTAC	7 cut(s)	57, 184, 433, 1936, 3060, 4308, 4340
RsaNI	GTAC	7 cut(s)	56, 183, 432, 1935, 3059, 4307, 4339
RseI	CAYNNNNRTG	7 cut(s)	63, 1344, 1496, 3838, 4149, 4284, 4314
SapI	GCTCTTC	1 cut(s)	2314
Sau96I	GGNCC	3 cut(s)	219, 3770, 4421
SchI	GAGTC	7 cut(s)	1380, 2348, 2431, 2458, 2967, 3315, 3605
SduI	GDGCHC	1 cut(s)	239
SexAI	ACCWGGT	1 cut(s)	3400
SfcI	CTRYAG	4 cut(s)	352, 1275, 2589, 3187
Sfr274I	CTCGAG	1 cut(s)	3303
SfuI	TTCGAA	1 cut(s)	937
SgrAI	CRCCGGYG	1 cut(s)	3671
SinI	GGWCC	3 cut(s)	219, 3770, 4421
SlaI	CTCGAG	1 cut(s)	3303
SmiMI	CAYNNNNRTG	7 cut(s)	63, 1344, 1496, 3838, 4149, 4284, 4314
SmlI	CTYRAG	6 cut(s)	886, 1043, 1831, 3303, 3415, 4081
SmoI	CTYRAG	6 cut(s)	886, 1043, 1831, 3303, 3415, 4081
SpeI	ACTAGT	3 cut(s)	28, 736, 2230
SseBI	AGGCCT	1 cut(s)	1213
SsiI	CCGC	6 cut(s)	756, 991, 2355, 2450, 2643, 3120
StuI	AGGCCT	1 cut(s)	1213
StyI	CCWWGG	5 cut(s)	1075, 2212, 2921, 3023, 3858
TaiI	ACGT	2 cut(s)	2299, 3534
TatI	WGTACW	3 cut(s)	431, 1934, 4306
TauI	GCSGC	1 cut(s)	2646
TscAI	CASTG	9 cut(s)	173, 935, 1299, 1365, 1732, 2701, 3223, 3231, 3553
TseFI	GTSAC	7 cut(s)	640, 661, 1094, 2068, 2416, 3510, 3730
Tsp45I	GTSAC	7 cut(s)	640, 661, 1094, 2068, 2416, 3510, 3730
TspGWI	ACGGA	8 cut(s)	101, 222, 358, 858, 1576, 2532, 3843, 4328
TspRI	CASTG	9 cut(s)	173, 935, 1299, 1365, 1732, 2701, 3223, 3231, 3553
Vha464I	CTTAAG	1 cut(s)	4081
VneI	GTGCAC	1 cut(s)	235
VpaK11BI	GGWCC	3 cut(s)	219, 3770, 4421
VspI	ATTAAT	2 cut(s)	107, 2774
XapI	RAATTY	6 cut(s)	795, 1633, 1970, 2110, 3151, 4208
XbaI	TCTAGA	2 cut(s)	410, 2965
XceI	RCATGY	7 cut(s)	303, 1106, 1621, 1875, 1935, 3725, 4031
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1073
XhoI	CTCGAG	1 cut(s)	3303
XmaJI	CCTAGG	1 cut(s)	2921
XmnI	GAANNNNTTC	3 cut(s)	1736, 3394, 3578
Zsp2I	ATGCAT	3 cut(s)	2278, 3015, 3727

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.