Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0004020.1

Orthogroup ID

OG0006120

Physical Location & Seq

Genomic Coordinates Reverse (-)

72415 .. 77805

Gene Structure 5391 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2592 bp

atggaattgccagactactcattggttaagaatcttgtaatgaaggttaaaaaggagatgttctcatcttcatcttcatcatcagaacatgatgatgtatatcgtcttgtgtctccttctgcttacgacactgcttggctcgccatggttccgaatcctgaaaaaccagatcggcccttgttccggggatgcttgcaatgggtgctccggaaccaaaaaccaggaggcttctggggatcatcagagtcgacctctataagtaccttatactccctcacttcgactctcgcttgcattgttgcactcaccacctggagtgttggccacactgccattcaaaaggggttggggttcattcatgcaaatgcagaagagatactgaaagagcaaaatggctgctttcccgagtggttgggacaggagtactatgagacactgatgccatatcttgaagctttgccggagagttataatgagattttgctacaacagagtgaagattatgggaccctcatccagtttccatcagccaccgcctatgcttttatgaagacaggaaataagaatcttttggtgaatttgaattctgttgtccaaagatgtggatatggagttcctgcgatttaccccatggatgaagacctcgtaaagatttgtcttattaaccggattgagacactgggcctagcagagtatttcatggaggagataacgattctacttgatcaagttttcagcaccaccaaggttgactttgaactcttcatcgacgactccagtaccaagaagaggctcgaaattgaggcatggttccggcaagagcagcgccgcccgtcggcacctgatgcaactgtgaggatggacgtctacactttttctgttttgcggacaagttatgtgaacaatgaagggacagagatgacggtgaagcatggtctgcttccactagaactttacagagattcactagcatttcggctactgagactacatggctatactgtatctccttggaagttctgctggttcctccaagatgaagatatattggtgcacatagaagagcaccatgaaacgtttttgagtgccatgtataatgtattcagggcatcagatcttacttttgccggagaaagtcagcttgaagatgctaggttattctctacaagaatactggaaaaggaaacaacaaattccgagaaattgctagtagaccagattaagcatgaacttagtcatccatggcttgctcggttggatcatttggaacacaggaaatgcattgagagggaagatgctattcatccatggacaggaaaagccttttcgtatagactatcattttcgagtgatgctctgctgcaacaacttgccatagagaattattcactcagacaatcaatattcagagatgaactaaccgagttagagaggtggtcaaaggacatgggactttgtgacatggggtttgcacgacagaaaaccgcacactgttattttgcagttgcatcaactgtatcacctccttccctttcggatgtgaggctggcacttgtcaaaagtgcaatccttgttacggtggctgatgatttttttgatgcggaaggttcattgagtgatctcaaaagttttacaaatgccgtcaaaagacgtcagcagataggcctgaatggacacagcaaaactatatttaaagctctcaaggatctcctcgatgacatttctatgcaattcttcaataaatatggatatgacgtaaacatataccttcaagatctagaggcagagtggagcagtactgggcataccccatcaatagctgagtaccttcaagtagccacgagttcaatagctgcacaaactattattcttccagcagctcttcttcttagtccacaacccggaatagacatcctcaagtgccctcaaaatgagccgcttacaaatttacttatggtttcaacacgctttttgaacgatattggaacctataagaaaaaaatcactgatgccccggatgcattaatattccagcgggaacaagaggaggggaagccaaaccttgttctgctacacatgaaggaaaatccggatctgggagttgaagaatcaattgaggttatacagaagatattggacgaaaagaagaaacagttcctggaacaggttttggtgaatggagtgaatgatatgccagaagcatgtaaactactccactttcaatgcctgaaagcatttcagatgttctacaactcaacaaatgcatttgactcccaaacagagttgcttgctgacatcaacaaagcgatttattttccattaaaggtggatcatagaagtttggctagtagccatagccaagttggtactaaaagccttcaaatccaacagagagtaattggaacaagttcaacattctcgaagagcattactgagaacagaacttgtactactggtgctataaagttcctccaaggaaagaagttttctgcaaagccttacgcggggatcttatcacttaagatgagaaaatcaataactcctacgactttcccaaagccgaaagcttgtacttattcgtggcataagtaa

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0000304 GO:0001101 GO:0003674 GO:0003824 GO:0006629 GO:0006720 GO:0006721 GO:0006950 GO:0006979 GO:0007584 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009719 GO:0009725 GO:0009753 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0016101 GO:0016102 GO:0016114 GO:0016829 GO:0016835 GO:0016838 GO:0031667 GO:0042221 GO:0042493 GO:0043207 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0080013 GO:0080027 GO:1901576 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00904 map00904

Pfam Domains

Protein Families

Terpene_synth Terpene_synth_C

Protein Analysis

863

Amino Acids

98.09

Weight (kDa)

5.75

Isoelectric Point (pI)

46.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Terpene_synth	PF01397	175 - 249	6e-07	Terpene synthase, N-terminal domain
Terpene_synth	PF01397	311 - 411	1.8e-17	Terpene synthase, N-terminal domain
Terpene_synth_C	PF03936	481 - 717	2.2e-39	Terpene synthase family, metal binding domain
Terpene_syn_C_2	PF19086	521 - 717	1.6e-23	Terpene synthase family 2, C-terminal metal binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006120)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G61120
malus_domestica	MD17G1080300.v1.1 MD17G1080400.v1.1 MD17G1080500.v1.1 MD17G1080700.v1.1 MD17G1080800.v1.1
prunus_persica	Prupe.3G234300_v2.0.a1 Prupe.3G234400_v2.0.a1 Prupe.3G234400_v2.0.a1 Prupe.3G234500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0162311
rosa_laevigata	RLG00000021334 RLG00000021337
rosa_multiflora	Rmu_co8462281.1_g000001 Rmu_sc0004020.1_g000013
rosa_roxburghii	Rroxscaffold_2G00088040 Rroxscaffold_2G00088060 Rroxscaffold_2G00088110
rosa_rugosa	Rorug02G0496200 Rorug02G0496300
rosa_samantha	Rh2AG562300 Rh2AG562400 Rh2BG575400 Rh2CG545800 Rh2DG585200 Rh2DG585600
rosa_wichuraiana	Rw2G046570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	471
AatII	GACGTC	2 cut(s)	867, 1654
AccB1I	GGYRCC	1 cut(s)	838
AccI	GTMKAC	3 cut(s)	248, 867, 1212
AccII	CGCG	1 cut(s)	2504
AccIII	TCCGGA	2 cut(s)	207, 2089
AciI	CCGC	8 cut(s)	534, 829, 886, 1485, 1601, 1937, 2035, 2504
AclI	AACGTT	1 cut(s)	1076
AclWI	GGATC	6 cut(s)	244, 1266, 1713, 2100, 2337, 2516
AcoI	YGGCCR	1 cut(s)	322
AcsI	RAATTY	4 cut(s)	577, 583, 1192, 1945
AcyI	GRCGYC	2 cut(s)	864, 1651
AfaI	GTAC	8 cut(s)	262, 425, 781, 1798, 1826, 2368, 2449, 2572
AfiI	CCNNNNNNNGG	9 cut(s)	183, 789, 835, 1313, 1576, 1901, 2096, 2164, 2504
AflII	CTTAAG	1 cut(s)	2519
AjnI	CCWGG	3 cut(s)	220, 311, 2157
AloI	GAACNNNNNNTCC	2 cut(s)	597, 629
AluBI	AGCT	7 cut(s)	455, 1141, 1697, 1820, 1853, 1880, 2567
AluI	AGCT	7 cut(s)	455, 1141, 1697, 1820, 1853, 1880, 2567
Alw21I	GWGCWC	3 cut(s)	207, 1056, 1068
Alw26I	GTCTC	4 cut(s)	117, 425, 668, 979
Alw44I	GTGCAC	1 cut(s)	1052
AlwI	GGATC	6 cut(s)	244, 1266, 1713, 2100, 2337, 2516
AlwNI	CAGNNNCTG	1 cut(s)	2158
Ama87I	CYCGRG	1 cut(s)	404
Aor13HI	TCCGGA	2 cut(s)	207, 2089
AoxI	GGCC	4 cut(s)	173, 322, 682, 1663
ApaLI	GTGCAC	1 cut(s)	1052
ApeKI	GCWGC	5 cut(s)	396, 823, 1360, 1853, 1877
ApoI	RAATTY	4 cut(s)	577, 583, 1192, 1945
ArsI	GACNNNNNNTTYG	2 cut(s)	1451, 1483
AseI	ATTAAT	1 cut(s)	2024
Asp700I	GAANNNNTTC	2 cut(s)	2153, 2407
AspLEI	GCGC	1 cut(s)	828
AspS9I	GGNCC	3 cut(s)	174, 507, 682
AsuC2I	CCSGG	3 cut(s)	185, 1902, 2015
AsuHPI	GGTGA	5 cut(s)	298, 586, 937, 1512, 2185
AvaI	CYCGRG	1 cut(s)	404
AvaII	GGWCC	1 cut(s)	507
BaeGI	GKGCMC	2 cut(s)	1056, 1925
BaeI	ACNNNNGTAYC	2 cut(s)	763, 796
BalI	TGGCCA	1 cut(s)	324
BanI	GGYRCC	1 cut(s)	838
BauI	CACGAG	1 cut(s)	1840
BbsI	GAAGAC	2 cut(s)	557, 645
Bbv12I	GWGCWC	3 cut(s)	207, 1056, 1068
BbvI	GCAGC	5 cut(s)	383, 835, 1347, 1840, 1889
BccI	CCATC	3 cut(s)	532, 853, 1819
BceAI	ACGGC	1 cut(s)	1625
BciT130I	CCWGG	3 cut(s)	222, 313, 2159
BclI	TGATCA	1 cut(s)	724
BcnI	CCSGG	3 cut(s)	185, 1902, 2015
BcoDI	GTCTC	4 cut(s)	117, 425, 668, 979
BfaI	CTAG	7 cut(s)	686, 947, 968, 1152, 1208, 1778, 2346
BfoI	RGCGCY	1 cut(s)	829
BfrI	CTTAAG	1 cut(s)	2519
BglII	AGATCT	2 cut(s)	1114, 1774
BisI	GCNGC	7 cut(s)	397, 824, 829, 1361, 1854, 1878, 1937
BlsI	GCNGC	7 cut(s)	398, 825, 830, 1362, 1855, 1879, 1938
BmcAI	AGTACT	2 cut(s)	425, 1798
Bme1390I	CCNGG	6 cut(s)	185, 222, 313, 1902, 2015, 2159
Bme18I	GGWCC	1 cut(s)	507
BmeT110I	CYCGRG	1 cut(s)	404
BmgT120I	GGNCC	3 cut(s)	174, 507, 682
BmiI	GGNNCC	8 cut(s)	150, 212, 508, 509, 812, 840, 1028, 1987
BmrFI	CCNGG	6 cut(s)	185, 222, 313, 1902, 2015, 2159
BmrI	ACTGGG	2 cut(s)	689, 1809
BmuI	ACTGGG	2 cut(s)	689, 1809
BpiI	GAAGAC	2 cut(s)	557, 645
BplI	GAGNNNNNCTC	6 cut(s)	47, 79, 236, 268, 1339, 1371
BpmI	CTGGAG	2 cut(s)	334, 760
BpuEI	CTTGAG	2 cut(s)	1685, 1901
BpuMI	CCSGG	3 cut(s)	185, 1902, 2015
BsaBI	GATNNNNATC	1 cut(s)	99
BsaHI	GRCGYC	2 cut(s)	864, 1651
BsaJI	CCNNGG	9 cut(s)	144, 184, 630, 744, 1010, 1241, 1307, 2013, 2473
BsaWI	WCCGGW	3 cut(s)	207, 666, 2089
BsaXI	ACNNNNNCTCC	2 cut(s)	991, 1021
Bsc4I	CCNNNNNNNGG	9 cut(s)	183, 789, 835, 1313, 1576, 1901, 2096, 2164, 2504
Bse1I	ACTGG	6 cut(s)	517, 684, 777, 1179, 1804, 2458
Bse3DI	GCAATG	1 cut(s)	203
Bse8I	GATNNNNATC	1 cut(s)	99
BseAI	TCCGGA	2 cut(s)	207, 2089
BseBI	CCWGG	3 cut(s)	222, 313, 2159
BseDI	CCNNGG	9 cut(s)	144, 184, 630, 744, 1010, 1241, 1307, 2013, 2473
BseGI	GGATG	9 cut(s)	194, 513, 640, 864, 1237, 1303, 1543, 1911, 2023
BseJI	GATNNNNATC	1 cut(s)	99
BseLI	CCNNNNNNNGG	9 cut(s)	183, 789, 835, 1313, 1576, 1901, 2096, 2164, 2504
BseMI	GCAATG	1 cut(s)	203
BseMII	CTCAG	4 cut(s)	974, 1405, 1812, 2424
BseNI	ACTGG	6 cut(s)	517, 684, 777, 1179, 1804, 2458
BseRI	GAGGAG	3 cut(s)	719, 1700, 2060
BseSI	GKGCMC	2 cut(s)	1056, 1925
BseXI	GCAGC	5 cut(s)	383, 835, 1347, 1840, 1889
BsgI	GTGCAG	1 cut(s)	1839
Bsh1236I	CGCG	1 cut(s)	2504
BshFI	GGCC	4 cut(s)	175, 324, 684, 1665
BshNI	GGYRCC	1 cut(s)	838
BsiHKAI	GWGCWC	3 cut(s)	207, 1056, 1068
BsiHKCI	CYCGRG	1 cut(s)	404
BsiSI	CCGG	9 cut(s)	184, 208, 461, 667, 814, 1128, 1902, 2015, 2090
BslFI	GGGAC	4 cut(s)	429, 520, 925, 1464
BslI	CCNNNNNNNGG	9 cut(s)	183, 789, 835, 1313, 1576, 1901, 2096, 2164, 2504
BsmAI	GTCTC	4 cut(s)	117, 425, 668, 979
BsmFI	GGGAC	4 cut(s)	429, 520, 925, 1464
BsnI	GGCC	4 cut(s)	175, 324, 684, 1665
BsoBI	CYCGRG	1 cut(s)	404
Bsp1286I	GDGCHC	4 cut(s)	207, 1056, 1068, 1925
Bsp13I	TCCGGA	2 cut(s)	207, 2089
Bsp19I	CCATGG	4 cut(s)	144, 630, 1241, 1307
BspACI	CCGC	8 cut(s)	534, 829, 886, 1485, 1601, 1937, 2035, 2504
BspANI	GGCC	4 cut(s)	175, 324, 684, 1665
BspCNI	CTCAG	4 cut(s)	975, 1404, 1813, 2425
BspEI	TCCGGA	2 cut(s)	207, 2089
BspFNI	CGCG	1 cut(s)	2504
BspLI	GGNNCC	8 cut(s)	150, 212, 508, 509, 812, 840, 1028, 1987
BspPI	GGATC	6 cut(s)	244, 1266, 1713, 2100, 2337, 2516
BspQI	GCTCTTC	3 cut(s)	1056, 1887, 2417
BspT107I	GGYRCC	1 cut(s)	838
BspTI	CTTAAG	1 cut(s)	2519
BsrDI	GCAATG	1 cut(s)	203
BsrI	ACTGG	6 cut(s)	517, 684, 777, 1179, 1804, 2458
BssECI	CCNNGG	9 cut(s)	144, 184, 630, 744, 1010, 1241, 1307, 2013, 2473
BssNI	GRCGYC	2 cut(s)	864, 1651
BssSI	CACGAG	1 cut(s)	1840
BssT1I	CCWWGG	7 cut(s)	144, 630, 744, 1010, 1241, 1307, 2473
Bst2BI	CACGAG	1 cut(s)	1840
Bst2UI	CCWGG	3 cut(s)	222, 313, 2159
Bst4CI	ACNGT	7 cut(s)	853, 925, 1003, 1493, 1516, 1579, 2154
Bst6I	CTCTTC	6 cut(s)	366, 767, 782, 1056, 1887, 2417
BstACI	GRCGYC	2 cut(s)	864, 1651
BstAFI	CTTAAG	1 cut(s)	2519
BstAPI	GCANNNNNTGC	3 cut(s)	202, 845, 937
BstC8I	GCNNGC	6 cut(s)	141, 194, 292, 1248, 1548, 2289
BstDEI	CTNAG	6 cut(s)	983, 1232, 1391, 1821, 1889, 2433
BstDSI	CCRYGG	4 cut(s)	144, 630, 1241, 1307
BstENI	CCTNNNNNAGG	1 cut(s)	2162
BstF5I	GGATG	9 cut(s)	194, 513, 640, 864, 1237, 1303, 1543, 1911, 2023
BstFNI	CGCG	1 cut(s)	2504
BstH2I	RGCGCY	1 cut(s)	829
BstHHI	GCGC	1 cut(s)	828
BstMAI	GTCTC	4 cut(s)	117, 425, 668, 979
BstMWI	GCNNNNNNNGC	6 cut(s)	140, 202, 823, 845, 937, 2018
BstNI	CCWGG	3 cut(s)	222, 313, 2159
BstNSI	RCATGY	1 cut(s)	2205
BstSCI	CCNGG	6 cut(s)	183, 220, 311, 1900, 2013, 2157
BstSLI	GKGCMC	2 cut(s)	1056, 1925
BstUI	CGCG	1 cut(s)	2504
BstV1I	GCAGC	5 cut(s)	383, 835, 1347, 1840, 1889
BstV2I	GAAGAC	2 cut(s)	557, 645
BstX2I	RGATCY	5 cut(s)	1114, 1705, 1774, 2092, 2508
BstXI	CCANNNNNNTGG	1 cut(s)	602
BstYI	RGATCY	5 cut(s)	1114, 1705, 1774, 2092, 2508
BsuRI	GGCC	4 cut(s)	175, 324, 684, 1665
BtgI	CCRYGG	4 cut(s)	144, 630, 1241, 1307
BtsCI	GGATG	9 cut(s)	194, 513, 640, 864, 1237, 1303, 1543, 1911, 2023
BtsI	GCAGTG	2 cut(s)	129, 327
BtsIMutI	CAGTG	6 cut(s)	129, 327, 434, 677, 1489, 2004
Cac8I	GCNNGC	6 cut(s)	141, 194, 292, 1248, 1548, 2289
CaiI	CAGNNNCTG	1 cut(s)	2158
CfoI	GCGC	1 cut(s)	828
Cfr13I	GGNCC	3 cut(s)	174, 507, 682
Csp6I	GTAC	8 cut(s)	261, 424, 780, 1797, 1825, 2367, 2448, 2571
CspCI	CAANNNNNGTGG	2 cut(s)	730, 765
CviQI	GTAC	8 cut(s)	261, 424, 780, 1797, 1825, 2367, 2448, 2571
DdeI	CTNAG	6 cut(s)	983, 1232, 1391, 1821, 1889, 2433
DraI	TTTAAA	1 cut(s)	1693
EaeI	YGGCCR	1 cut(s)	322
Eam1104I	CTCTTC	6 cut(s)	366, 767, 782, 1056, 1887, 2417
EarI	CTCTTC	6 cut(s)	366, 767, 782, 1056, 1887, 2417
Eco130I	CCWWGG	7 cut(s)	144, 630, 744, 1010, 1241, 1307, 2473
Eco147I	AGGCCT	1 cut(s)	1665
Eco47I	GGWCC	1 cut(s)	507
Eco88I	CYCGRG	1 cut(s)	404
EcoNI	CCTNNNNNAGG	1 cut(s)	2162
EcoO109I	RGGNCCY	1 cut(s)	507
EcoRI	GAATTC	1 cut(s)	583
EcoRII	CCWGG	3 cut(s)	220, 311, 2157
EcoT14I	CCWWGG	7 cut(s)	144, 630, 744, 1010, 1241, 1307, 2473
EcoT22I	ATGCAT	3 cut(s)	1283, 2023, 2266
ErhI	CCWWGG	7 cut(s)	144, 630, 744, 1010, 1241, 1307, 2473
FalI	AAGNNNNNCTT	2 cut(s)	737, 769
FaqI	GGGAC	4 cut(s)	429, 520, 925, 1464
FauI	CCCGC	2 cut(s)	2028, 2497
FbaI	TGATCA	1 cut(s)	724
FblI	GTMKAC	3 cut(s)	248, 867, 1212
Fnu4HI	GCNGC	7 cut(s)	397, 824, 829, 1361, 1854, 1878, 1937
FokI	GGATG	9 cut(s)	201, 500, 647, 871, 1224, 1290, 1550, 1898, 2030
Fsp4HI	GCNGC	7 cut(s)	397, 824, 829, 1361, 1854, 1878, 1937
FspBI	CTAG	7 cut(s)	686, 947, 968, 1152, 1208, 1778, 2346
GlaI	GCGC	1 cut(s)	827
GluI	GCNGC	7 cut(s)	397, 824, 829, 1361, 1854, 1878, 1937
GsuI	CTGGAG	2 cut(s)	334, 760
HaeII	RGCGCY	1 cut(s)	829
HaeIII	GGCC	4 cut(s)	175, 324, 684, 1665
HapII	CCGG	9 cut(s)	184, 208, 461, 667, 814, 1128, 1902, 2015, 2090
HhaI	GCGC	1 cut(s)	828
Hin1I	GRCGYC	2 cut(s)	864, 1651
Hin6I	GCGC	1 cut(s)	826
HinP1I	GCGC	1 cut(s)	826
HincII	GTYRAC	2 cut(s)	249, 751
HindII	GTYRAC	2 cut(s)	249, 751
HindIII	AAGCTT	2 cut(s)	453, 2565
HpaII	CCGG	9 cut(s)	184, 208, 461, 667, 814, 1128, 1902, 2015, 2090
HphI	GGTGA	5 cut(s)	298, 586, 937, 1512, 2185
Hpy166II	GTNNAC	9 cut(s)	249, 751, 868, 901, 1054, 1213, 1759, 1895, 2207
Hpy188I	TCNGA	9 cut(s)	85, 153, 244, 1114, 1198, 1394, 1409, 1537, 2241
Hpy188III	TCNNGA	8 cut(s)	158, 208, 404, 449, 1772, 1778, 2090, 2419
Hpy8I	GTNNAC	9 cut(s)	249, 751, 868, 901, 1054, 1213, 1759, 1895, 2207
Hpy99I	CGWCG	2 cut(s)	773, 838
HpyAV	CCTTC	9 cut(s)	37, 126, 902, 1536, 1598, 1778, 1838, 2074, 2387
HpyCH4III	ACNGT	7 cut(s)	853, 925, 1003, 1493, 1516, 1579, 2154
HpyCH4IV	ACGT	4 cut(s)	864, 1076, 1651, 1755
HpyF10VI	GCNNNNNNNGC	6 cut(s)	140, 202, 823, 845, 937, 2018
HpyF3I	CTNAG	6 cut(s)	983, 1232, 1391, 1821, 1889, 2433
HpySE526I	ACGT	4 cut(s)	864, 1076, 1651, 1755
Hsp92I	GRCGYC	2 cut(s)	864, 1651
HspAI	GCGC	1 cut(s)	826
KflI	GGGWCCC	1 cut(s)	507
Kpn2I	TCCGGA	2 cut(s)	207, 2089
Ksp22I	TGATCA	1 cut(s)	724
LguI	GCTCTTC	3 cut(s)	1056, 1887, 2417
LmnI	GCTCC	2 cut(s)	210, 1791
Lsp1109I	GCAGC	5 cut(s)	383, 835, 1347, 1840, 1889
MaeI	CTAG	7 cut(s)	686, 947, 968, 1152, 1208, 1778, 2346
MaeII	ACGT	4 cut(s)	864, 1076, 1651, 1755
MaeIII	GTNAC	2 cut(s)	1457, 1573
MfeI	CAATTG	1 cut(s)	2112
MflI	RGATCY	5 cut(s)	1114, 1705, 1774, 2092, 2508
MhlI	GDGCHC	4 cut(s)	207, 1056, 1068, 1925
MlsI	TGGCCA	1 cut(s)	324
MluNI	TGGCCA	1 cut(s)	324
MlyI	GAGTC	4 cut(s)	254, 277, 767, 2264
MmeI	TCCRAC	2 cut(s)	1236, 2410
Mox20I	TGGCCA	1 cut(s)	324
Mph1103I	ATGCAT	3 cut(s)	1283, 2023, 2266
MroI	TCCGGA	2 cut(s)	207, 2089
MroXI	GAANNNNTTC	2 cut(s)	2153, 2407
MscI	TGGCCA	1 cut(s)	324
MseI	TTAA	8 cut(s)	27, 48, 663, 1221, 1692, 2024, 2321, 2520
MslI	CAYNNNNRTG	3 cut(s)	93, 363, 1724
Msp20I	TGGCCA	1 cut(s)	324
MspA1I	CMGCKG	1 cut(s)	2035
MspCI	CTTAAG	1 cut(s)	2519
MspI	CCGG	9 cut(s)	184, 208, 461, 667, 814, 1128, 1902, 2015, 2090
MspR9I	CCNGG	6 cut(s)	185, 222, 313, 1902, 2015, 2159
MunI	CAATTG	1 cut(s)	2112
MvaI	CCWGG	3 cut(s)	222, 313, 2159
MvnI	CGCG	1 cut(s)	2504
MwoI	GCNNNNNNNGC	6 cut(s)	140, 202, 823, 845, 937, 2018
NciI	CCSGG	3 cut(s)	185, 1902, 2015
NcoI	CCATGG	4 cut(s)	144, 630, 1241, 1307
NlaIV	GGNNCC	8 cut(s)	150, 212, 508, 509, 812, 840, 1028, 1987
NmuCI	GTSAC	1 cut(s)	1457
NsiI	ATGCAT	3 cut(s)	1283, 2023, 2266
NspI	RCATGY	1 cut(s)	2205
PceI	AGGCCT	1 cut(s)	1665
PciSI	GCTCTTC	3 cut(s)	1056, 1887, 2417
PdmI	GAANNNNTTC	2 cut(s)	2153, 2407
PfeI	GAWTC	6 cut(s)	31, 154, 565, 715, 962, 2108
PfoI	TCCNGGA	1 cut(s)	2157
PkrI	GCNGC	7 cut(s)	398, 825, 830, 1362, 1855, 1879, 1938
PleI	GAGTC	4 cut(s)	253, 277, 767, 2264
PpsI	GAGTC	4 cut(s)	253, 277, 767, 2264
PpuMI	RGGWCCY	1 cut(s)	507
PshBI	ATTAAT	1 cut(s)	2024
PsiI	TTATAA	1 cut(s)	471
Psp1406I	AACGTT	1 cut(s)	1076
Psp5II	RGGWCCY	1 cut(s)	507
Psp6I	CCWGG	3 cut(s)	220, 311, 2157
PspGI	CCWGG	3 cut(s)	220, 311, 2157
PspN4I	GGNNCC	8 cut(s)	150, 212, 508, 509, 812, 840, 1028, 1987
PspPI	GGNCC	3 cut(s)	174, 507, 682
PspPPI	RGGWCCY	1 cut(s)	507
PsrI	GAACNNNNNNTAC	2 cut(s)	2434, 2466
PstNI	CAGNNNCTG	1 cut(s)	2158
PsuI	RGATCY	5 cut(s)	1114, 1705, 1774, 2092, 2508
RsaI	GTAC	8 cut(s)	262, 425, 781, 1798, 1826, 2368, 2449, 2572
RsaNI	GTAC	8 cut(s)	261, 424, 780, 1797, 1825, 2367, 2448, 2571
RseI	CAYNNNNRTG	3 cut(s)	93, 363, 1724
SalI	GTCGAC	1 cut(s)	247
SapI	GCTCTTC	3 cut(s)	1056, 1887, 2417
SaqAI	TTAA	8 cut(s)	27, 48, 663, 1221, 1692, 2024, 2321, 2520
SatI	GCNGC	7 cut(s)	397, 824, 829, 1361, 1854, 1878, 1937
Sau96I	GGNCC	3 cut(s)	174, 507, 682
ScaI	AGTACT	2 cut(s)	425, 1798
SchI	GAGTC	4 cut(s)	254, 277, 767, 2264
ScrFI	CCNGG	6 cut(s)	185, 222, 313, 1902, 2015, 2159
SduI	GDGCHC	4 cut(s)	207, 1056, 1068, 1925
SinI	GGWCC	1 cut(s)	507
SmiMI	CAYNNNNRTG	3 cut(s)	93, 363, 1724
SmlI	CTYRAG	3 cut(s)	1700, 1916, 2519
SmoI	CTYRAG	3 cut(s)	1700, 1916, 2519
SseBI	AGGCCT	1 cut(s)	1665
SsiI	CCGC	8 cut(s)	534, 829, 886, 1485, 1601, 1937, 2035, 2504
SspI	AATATT	2 cut(s)	1404, 2028
SspMI	CTAG	7 cut(s)	686, 947, 968, 1152, 1208, 1778, 2346
StuI	AGGCCT	1 cut(s)	1665
StyD4I	CCNGG	6 cut(s)	183, 220, 311, 1900, 2013, 2157
StyI	CCWWGG	7 cut(s)	144, 630, 744, 1010, 1241, 1307, 2473
TaaI	ACNGT	7 cut(s)	853, 925, 1003, 1493, 1516, 1579, 2154
TaiI	ACGT	4 cut(s)	867, 1079, 1654, 1758
TaqI	TCGA	7 cut(s)	248, 281, 768, 795, 1346, 1713, 2420
TatI	WGTACW	4 cut(s)	423, 1796, 2447, 2570
TauI	GCSGC	2 cut(s)	831, 1939
TfiI	GAWTC	6 cut(s)	31, 154, 565, 715, 962, 2108
Tru1I	TTAA	8 cut(s)	27, 48, 663, 1221, 1692, 2024, 2321, 2520
Tru9I	TTAA	8 cut(s)	27, 48, 663, 1221, 1692, 2024, 2321, 2520
TscAI	CASTG	6 cut(s)	136, 334, 441, 684, 1496, 2011
TseFI	GTSAC	1 cut(s)	1457
TseI	GCWGC	5 cut(s)	396, 823, 1360, 1853, 1877
Tsp45I	GTSAC	1 cut(s)	1457
TspRI	CASTG	6 cut(s)	136, 334, 441, 684, 1496, 2011
Vha464I	CTTAAG	1 cut(s)	2519
VneI	GTGCAC	1 cut(s)	1052
VpaK11BI	GGWCC	1 cut(s)	507
VspI	ATTAAT	1 cut(s)	2024
XagI	CCTNNNNNAGG	1 cut(s)	2162
XapI	RAATTY	4 cut(s)	577, 583, 1192, 1945
XbaI	TCTAGA	1 cut(s)	1777
XceI	RCATGY	1 cut(s)	2205
XcmI	CCANNNNNNNNNTGG	2 cut(s)	228, 2360
XmiI	GTMKAC	3 cut(s)	248, 867, 1212
XmnI	GAANNNNTTC	2 cut(s)	2153, 2407
XspI	CTAG	7 cut(s)	686, 947, 968, 1152, 1208, 1778, 2346
ZraI	GACGTC	2 cut(s)	865, 1652
ZrmI	AGTACT	2 cut(s)	425, 1798
Zsp2I	ATGCAT	3 cut(s)	1283, 2023, 2266

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.