Rmu_sc0004596.1_g000037

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004596.1
OG0014716
Physical Location & Seq
Reverse (-)
155560 .. 156476
917 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 837 bp
atggagccacctaggcaagcttccatggatacagaatggatatattatcccagaaactctgaaacctaccgtgctggagcccaattgtatgttgataaacttcacaccagcttggggaatccagctatcgtgcaatgcccttgtcgcctctacaaaaatatgcagtggcattcccctaagttagtttttggtcacttgtgcatgaatgagatccatcttcttcatgttagtcggatcttacatggtgagtcgacaacatatgtacaagaagagaatgttcaagttccagaaatgtacagaattttgaaagatgcatactttcaagatgatgttgttatggaacctgaaaatgaaaggaatgaggctgactttagagatatattagaggaggaagaactaccattatattcagggtctgattttactaagatgtcttccacaatttccttgtttaagtataaggttcgacacagtttacctgataatgcttttgatgacctcctccaattggttgggggattactaccaacagagaacattcttccaaattccttgtattcaactaaaaaactcttgaaagtatttgatctaggttaccagaagatacatgcttgtgtaagggattgtgtattgtttagaaaagaactagagcatgtggagacctatccaaaatgtggttcatcgaggtggaaggttcataaacacaccaaaaaagttagtaaaggagttccggcaaaggtgttgaggtattttacaatcatacccaggtttagaaggatgtttaagtcagtagagatggctgaacaattgacttggcactacaatcacagaagttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218 Transpos_assoc Transposase_21

Protein Analysis

278

Amino Acids

32.72

Weight (kDa)

8.12

Isoelectric Point (pI)

47.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transpos_assoc PF13963 12 - 83 4.2e-07 Transposase-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 674
AccI GTMKAC 1 cut(s) 251
AclWI GGATC 2 cut(s) 205, 242
AcsI RAATTY 2 cut(s) 300, 547
AfaI GTAC 2 cut(s) 264, 296
AfiI CCNNNNNNNGG 3 cut(s) 114, 508, 674
AgsI TTSAA 5 cut(s) 281, 307, 323, 561, 577
AjnI CCWGG 1 cut(s) 764
AjuI GAANNNNNNNTTGG 2 cut(s) 661, 693
AluBI AGCT 3 cut(s) 20, 111, 125
AluI AGCT 3 cut(s) 20, 111, 125
Alw26I GTCTC 1 cut(s) 653
AlwI GGATC 2 cut(s) 205, 242
AlwNI CAGNNNCTG 1 cut(s) 416
ApoI RAATTY 2 cut(s) 300, 547
AspA2I CCTAGG 1 cut(s) 11
AsuHPI GGTGA 1 cut(s) 257
AvrII CCTAGG 1 cut(s) 11
BanII GRGCYC 1 cut(s) 82
BbsI GAAGAC 1 cut(s) 426
BccI CCATC 2 cut(s) 222, 790
BciT130I CCWGG 1 cut(s) 766
BciVI GTATCC 1 cut(s) 22
BcoDI GTCTC 1 cut(s) 653
BfaI CTAG 3 cut(s) 12, 590, 647
BfuI GTATCC 1 cut(s) 22
BglI GCCNNNNNGGC 1 cut(s) 13
BlnI CCTAGG 1 cut(s) 11
Bme1390I CCNGG 1 cut(s) 766
BmiI GGNNCC 3 cut(s) 6, 79, 342
BmrFI CCNGG 1 cut(s) 766
BmsI GCATC 1 cut(s) 301
BpiI GAAGAC 1 cut(s) 426
BpmI CTGGAG 1 cut(s) 96
BsaI GGTCTC 1 cut(s) 653
BsaJI CCNNGG 3 cut(s) 11, 24, 764
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 508, 674
Bse3DI GCAATG 1 cut(s) 140
BseBI CCWGG 1 cut(s) 766
BseDI CCNNGG 3 cut(s) 11, 24, 764
BseGI GGATG 1 cut(s) 783
BseLI CCNNNNNNNGG 3 cut(s) 114, 508, 674
BseMI GCAATG 1 cut(s) 140
BseRI GAGGAG 2 cut(s) 401, 491
BsiSI CCGG 1 cut(s) 731
BslI CCNNNNNNNGG 3 cut(s) 114, 508, 674
BsmAI GTCTC 1 cut(s) 653
BsmI GAATGC 1 cut(s) 169
Bso31I GGTCTC 1 cut(s) 653
Bsp1286I GDGCHC 1 cut(s) 82
Bsp1407I TGTACA 2 cut(s) 262, 294
Bsp143I GATC 3 cut(s) 210, 234, 586
Bsp19I CCATGG 1 cut(s) 24
BspLI GGNNCC 3 cut(s) 6, 79, 342
BspPI GGATC 2 cut(s) 205, 242
BspTNI GGTCTC 1 cut(s) 653
BsrDI GCAATG 1 cut(s) 140
BsrGI TGTACA 2 cut(s) 262, 294
BssECI CCNNGG 3 cut(s) 11, 24, 764
BssMI GATC 3 cut(s) 210, 234, 586
BssT1I CCWWGG 2 cut(s) 11, 24
Bst2UI CCWGG 1 cut(s) 766
Bst4CI ACNGT 2 cut(s) 71, 473
Bst6I CTCTTC 1 cut(s) 264
BstAUI TGTACA 2 cut(s) 262, 294
BstC8I GCNNGC 1 cut(s) 18
BstDEI CTNAG 2 cut(s) 177, 426
BstDSI CCRYGG 1 cut(s) 24
BstEII GGTNACC 1 cut(s) 593
BstF5I GGATG 1 cut(s) 783
BstKTI GATC 3 cut(s) 213, 237, 589
BstMAI GTCTC 1 cut(s) 653
BstMBI GATC 3 cut(s) 210, 234, 586
BstMWI GCNNNNNNNGC 2 cut(s) 13, 144
BstNI CCWGG 1 cut(s) 766
BstNSI RCATGY 2 cut(s) 611, 656
BstPI GGTNACC 1 cut(s) 593
BstSCI CCNGG 1 cut(s) 764
BstV2I GAAGAC 1 cut(s) 426
BstX2I RGATCY 2 cut(s) 210, 234
BstXI CCANNNNNNTGG 1 cut(s) 512
BstYI RGATCY 2 cut(s) 210, 234
BsuI GTATCC 1 cut(s) 22
BtgI CCRYGG 1 cut(s) 24
BtsCI GGATG 1 cut(s) 783
BtsI GCAGTG 1 cut(s) 170
BtsIMutI CAGTG 1 cut(s) 170
Cac8I GCNNGC 1 cut(s) 18
CaiI CAGNNNCTG 1 cut(s) 416
Csp6I GTAC 2 cut(s) 263, 295
CviAII CATG 6 cut(s) 25, 202, 224, 242, 608, 653
CviJI RGCY 7 cut(s) 7, 20, 80, 111, 125, 365, 800
CviKI_1 RGCY 7 cut(s) 7, 20, 80, 111, 125, 365, 800
CviQI GTAC 2 cut(s) 263, 295
DdeI CTNAG 2 cut(s) 177, 426
DpnI GATC 3 cut(s) 212, 236, 588
DpnII GATC 3 cut(s) 210, 234, 586
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
Eco130I CCWWGG 2 cut(s) 11, 24
Eco24I GRGCYC 1 cut(s) 82
Eco31I GGTCTC 1 cut(s) 653
Eco91I GGTNACC 1 cut(s) 593
EcoO65I GGTNACC 1 cut(s) 593
EcoRII CCWGG 1 cut(s) 764
EcoT14I CCWWGG 2 cut(s) 11, 24
EcoT22I ATGCAT 1 cut(s) 316
EcoT38I GRGCYC 1 cut(s) 82
ErhI CCWWGG 2 cut(s) 11, 24
FaeI CATG 6 cut(s) 28, 205, 227, 245, 611, 656
FatI CATG 6 cut(s) 24, 201, 223, 241, 607, 652
FauNDI CATATG 1 cut(s) 259
FblI GTMKAC 1 cut(s) 251
FokI GGATG 1 cut(s) 790
FriOI GRGCYC 1 cut(s) 82
FspBI CTAG 3 cut(s) 12, 590, 647
GsuI CTGGAG 1 cut(s) 96
HapII CCGG 1 cut(s) 731
Hin1II CATG 6 cut(s) 28, 205, 227, 245, 611, 656
HincII GTYRAC 1 cut(s) 252
HindII GTYRAC 1 cut(s) 252
HindIII AAGCTT 1 cut(s) 18
HinfI GANTC 2 cut(s) 118, 248
HpaII CCGG 1 cut(s) 731
HphI GGTGA 1 cut(s) 257
Hpy166II GTNNAC 2 cut(s) 252, 476
Hpy188I TCNGA 3 cut(s) 61, 234, 418
Hpy188III TCNNGA 3 cut(s) 287, 323, 574
Hpy8I GTNNAC 2 cut(s) 252, 476
HpyAV CCTTC 2 cut(s) 685, 768
HpyCH4III ACNGT 2 cut(s) 71, 473
HpyCH4V TGCA 4 cut(s) 133, 163, 201, 314
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 144
HpyF3I CTNAG 2 cut(s) 177, 426
Hsp92II CATG 6 cut(s) 28, 205, 227, 245, 611, 656
Kzo9I GATC 3 cut(s) 210, 234, 586
LmnI GCTCC 2 cut(s) 4, 77
LweI GCATC 1 cut(s) 301
MaeI CTAG 3 cut(s) 12, 590, 647
MaeIII GTNAC 2 cut(s) 191, 593
MalI GATC 3 cut(s) 212, 236, 588
MboI GATC 3 cut(s) 210, 234, 586
MboII GAAGA 7 cut(s) 209, 212, 281, 404, 426, 533, 613
MfeI CAATTG 3 cut(s) 83, 506, 806
MflI RGATCY 2 cut(s) 210, 234
MhlI GDGCHC 1 cut(s) 82
MluCI AATT 6 cut(s) 83, 300, 441, 506, 547, 806
MlyI GAGTC 1 cut(s) 257
MmeI TCCRAC 1 cut(s) 212
MnlI CCTC 8 cut(s) 158, 355, 379, 382, 509, 512, 678, 738
Mph1103I ATGCAT 1 cut(s) 316
MseI TTAA 3 cut(s) 453, 783, 835
MslI CAYNNNNRTG 2 cut(s) 612, 685
MspI CCGG 1 cut(s) 731
MspR9I CCNGG 1 cut(s) 766
MunI CAATTG 3 cut(s) 83, 506, 806
Mva1269I GAATGC 1 cut(s) 169
MvaI CCWGG 1 cut(s) 766
MwoI GCNNNNNNNGC 2 cut(s) 13, 144
NcoI CCATGG 1 cut(s) 24
NdeI CATATG 1 cut(s) 259
NdeII GATC 3 cut(s) 210, 234, 586
NlaIII CATG 6 cut(s) 28, 205, 227, 245, 611, 656
NlaIV GGNNCC 3 cut(s) 6, 79, 342
NmuCI GTSAC 1 cut(s) 191
NsiI ATGCAT 1 cut(s) 316
NspI RCATGY 2 cut(s) 611, 656
PctI GAATGC 1 cut(s) 169
PfeI GAWTC 1 cut(s) 118
PflMI CCANNNNNTGG 1 cut(s) 674
PleI GAGTC 1 cut(s) 256
PpsI GAGTC 1 cut(s) 256
Psp6I CCWGG 1 cut(s) 764
PspEI GGTNACC 1 cut(s) 593
PspGI CCWGG 1 cut(s) 764
PspN4I GGNNCC 3 cut(s) 6, 79, 342
PstNI CAGNNNCTG 1 cut(s) 416
PsuI RGATCY 2 cut(s) 210, 234
RsaI GTAC 2 cut(s) 264, 296
RsaNI GTAC 2 cut(s) 263, 295
RseI CAYNNNNRTG 2 cut(s) 612, 685
SalI GTCGAC 1 cut(s) 250
SaqAI TTAA 3 cut(s) 453, 783, 835
Sau3AI GATC 3 cut(s) 210, 234, 586
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 1 cut(s) 766
SduI GDGCHC 1 cut(s) 82
SfaNI GCATC 1 cut(s) 301
SmiMI CAYNNNNRTG 2 cut(s) 612, 685
Sse9I AATT 6 cut(s) 83, 300, 441, 506, 547, 806
SspMI CTAG 3 cut(s) 12, 590, 647
StyD4I CCNGG 1 cut(s) 764
StyI CCWWGG 2 cut(s) 11, 24
TaaI ACNGT 2 cut(s) 71, 473
TaqI TCGA 3 cut(s) 251, 466, 683
TasI AATT 6 cut(s) 83, 300, 441, 506, 547, 806
TatI WGTACW 2 cut(s) 262, 294
TfiI GAWTC 1 cut(s) 118
Tru1I TTAA 3 cut(s) 453, 783, 835
Tru9I TTAA 3 cut(s) 453, 783, 835
TscAI CASTG 1 cut(s) 170
TseFI GTSAC 1 cut(s) 191
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 5 cut(s) 212, 218, 366, 669, 686
TspRI CASTG 1 cut(s) 170
Van91I CCANNNNNTGG 1 cut(s) 674
XapI RAATTY 2 cut(s) 300, 547
XceI RCATGY 2 cut(s) 611, 656
XmaJI CCTAGG 1 cut(s) 11
XmiI GTMKAC 1 cut(s) 251
XspI CTAG 3 cut(s) 12, 590, 647
Zsp2I ATGCAT 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.