Rmu_sc0004932.1_g000002

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004932.1
OG0006991
Physical Location & Seq
Forward (+)
1616 .. 2482
867 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 867 bp
atgcttaaaattagggagctttgtcgatctttctttgctaatatcattggtgatggtcgtactacttctttgtggtttgataattggcaccctcttggtcctcttactcttagatggtcatctaatatcattgctgattccgctctctctagctctgccatggtcagtgatgttatctataataataattggagatggccttctatctctcatgagttactagagattcgtgtttcattgacccaaatcattcctaactctgaggttagtgattctgttcgatggactctttcttcgaatggggtttattctacttcctctgcatggaatgccctccgtttttctaggcctattgttccttggtatcatttagtctggtttggtagttgtgttcctagatggagcttcatcttatggcttgctattagaggaaggttatcaactgtggatcgtgtaagatttttttctcctcttgctgcccatgaatgtgctttttgtcttgctactgatgagactcacaaccatcttttctttgagtgtgtttatacctctgccatttgggctcacattttgtctaaatgtgatgtgagtaatcctctcctttcttggcagcatttcattccatgggctgctgctaactggaagggtaaatctttttctattgcaatcaaaaagttggcattgcaagctgcagtttatgctatctggcgcgagagaaataaccgcaagttccgtaacgaaaacctcccttctgctgttgtgttcaaggcaattgtggagtccatacggttatgcttattgtcttggaagatccctccttctccttctaacagctatattattcatgaatggagactcccttcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04130 map04130
Pfam Domains
Protein Families
DUF4283 Exo_endo_phos RVT_1 zf-RVT

Protein Analysis

288

Amino Acids

33.21

Weight (kDa)

9.5

Isoelectric Point (pI)

46.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 103 - 187 5.4e-20 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 87
AccBSI CCGCTC 1 cut(s) 143
AccII CGCG 1 cut(s) 711
AciI CCGC 2 cut(s) 141, 724
AclWI GGATC 2 cut(s) 456, 805
AfaI GTAC 1 cut(s) 61
AfiI CCNNNNNNNGG 1 cut(s) 324
AgsI TTSAA 1 cut(s) 766
AluBI AGCT 5 cut(s) 19, 153, 405, 689, 834
AluI AGCT 5 cut(s) 19, 153, 405, 689, 834
Alw26I GTCTC 2 cut(s) 506, 847
AlwI GGATC 2 cut(s) 456, 805
AoxI GGCC 2 cut(s) 197, 347
ApeKI GCWGC 5 cut(s) 476, 610, 629, 632, 689
ArsI GACNNNNNNTTYG 2 cut(s) 277, 309
AspLEI GCGC 1 cut(s) 711
AspS9I GGNCC 1 cut(s) 98
AsuHPI GGTGA 1 cut(s) 62
AsuII TTCGAA 1 cut(s) 296
AvaII GGWCC 1 cut(s) 98
BanI GGYRCC 1 cut(s) 87
BanII GRGCYC 1 cut(s) 565
BbvI GCAGC 5 cut(s) 463, 616, 619, 622, 676
BccI CCATC 6 cut(s) 47, 108, 189, 276, 393, 531
BcoDI GTCTC 2 cut(s) 506, 847
BfaI CTAG 4 cut(s) 150, 221, 345, 396
BfmI CTRYAG 1 cut(s) 690
BglI GCCNNNNNGGC 1 cut(s) 560
BisI GCNGC 5 cut(s) 477, 611, 630, 633, 690
BlsI GCNGC 5 cut(s) 478, 612, 631, 634, 691
Bme18I GGWCC 1 cut(s) 98
BmgT120I GGNCC 1 cut(s) 98
BmiI GGNNCC 1 cut(s) 89
BplI GAGNNNNNCTC 2 cut(s) 580, 612
Bpu14I TTCGAA 1 cut(s) 296
BsaBI GATNNNNATC 1 cut(s) 118
BsaJI CCNNGG 3 cut(s) 159, 359, 623
Bsc4I CCNNNNNNNGG 1 cut(s) 324
Bse1I ACTGG 1 cut(s) 644
Bse3DI GCAATG 2 cut(s) 129, 680
Bse8I GATNNNNATC 1 cut(s) 118
BseDI CCNNGG 3 cut(s) 159, 359, 623
BseJI GATNNNNATC 1 cut(s) 118
BseLI CCNNNNNNNGG 1 cut(s) 324
BseMI GCAATG 2 cut(s) 129, 680
BseMII CTCAG 1 cut(s) 252
BseNI ACTGG 1 cut(s) 644
BseRI GAGGAG 1 cut(s) 459
BseXI GCAGC 5 cut(s) 463, 616, 619, 622, 676
Bsh1236I CGCG 1 cut(s) 711
BshFI GGCC 2 cut(s) 199, 349
BshNI GGYRCC 1 cut(s) 87
BslI CCNNNNNNNGG 1 cut(s) 324
BsmAI GTCTC 2 cut(s) 506, 847
BsmI GAATGC 1 cut(s) 334
BsnI GGCC 2 cut(s) 199, 349
Bsp119I TTCGAA 1 cut(s) 296
Bsp1286I GDGCHC 1 cut(s) 565
Bsp143I GATC 3 cut(s) 26, 448, 810
Bsp19I CCATGG 2 cut(s) 159, 623
BspACI CCGC 2 cut(s) 141, 724
BspANI GGCC 2 cut(s) 199, 349
BspCNI CTCAG 1 cut(s) 253
BspFNI CGCG 1 cut(s) 711
BspHI TCATGA 2 cut(s) 211, 844
BspLI GGNNCC 1 cut(s) 89
BspMAI CTGCAG 1 cut(s) 694
BspPI GGATC 2 cut(s) 456, 805
BspT104I TTCGAA 1 cut(s) 296
BspT107I GGYRCC 1 cut(s) 87
BsrBI CCGCTC 1 cut(s) 143
BsrDI GCAATG 2 cut(s) 129, 680
BsrI ACTGG 1 cut(s) 644
BssECI CCNNGG 3 cut(s) 159, 359, 623
BssMI GATC 3 cut(s) 26, 448, 810
BssT1I CCWWGG 3 cut(s) 159, 359, 623
Bst4CI ACNGT 2 cut(s) 445, 789
BstAPI GCANNNNNTGC 2 cut(s) 329, 698
BstBI TTCGAA 1 cut(s) 296
BstC8I GCNNGC 2 cut(s) 420, 687
BstDEI CTNAG 2 cut(s) 110, 261
BstDSI CCRYGG 2 cut(s) 159, 623
BstFNI CGCG 1 cut(s) 711
BstHHI GCGC 1 cut(s) 711
BstKTI GATC 3 cut(s) 29, 451, 813
BstMAI GTCTC 2 cut(s) 506, 847
BstMBI GATC 3 cut(s) 26, 448, 810
BstMWI GCNNNNNNNGC 5 cut(s) 140, 329, 560, 686, 698
BstSFI CTRYAG 1 cut(s) 690
BstUI CGCG 1 cut(s) 711
BstV1I GCAGC 5 cut(s) 463, 616, 619, 622, 676
BstX2I RGATCY 1 cut(s) 810
BstYI RGATCY 1 cut(s) 810
BsuRI GGCC 2 cut(s) 199, 349
BtgI CCRYGG 2 cut(s) 159, 623
BtsIMutI CAGTG 1 cut(s) 172
Cac8I GCNNGC 2 cut(s) 420, 687
CciI TCATGA 2 cut(s) 211, 844
CfoI GCGC 1 cut(s) 711
Cfr13I GGNCC 1 cut(s) 98
Csp6I GTAC 1 cut(s) 60
CviAII CATG 6 cut(s) 160, 212, 324, 482, 624, 845
CviQI GTAC 1 cut(s) 60
DdeI CTNAG 2 cut(s) 110, 261
DpnI GATC 3 cut(s) 28, 450, 812
DpnII GATC 3 cut(s) 26, 448, 810
Eco130I CCWWGG 3 cut(s) 159, 359, 623
Eco147I AGGCCT 1 cut(s) 349
Eco24I GRGCYC 1 cut(s) 565
Eco47I GGWCC 1 cut(s) 98
EcoT14I CCWWGG 3 cut(s) 159, 359, 623
EcoT38I GRGCYC 1 cut(s) 565
ErhI CCWWGG 3 cut(s) 159, 359, 623
FaeI CATG 6 cut(s) 163, 215, 327, 485, 627, 848
FatI CATG 6 cut(s) 159, 211, 323, 481, 623, 844
Fnu4HI GCNGC 5 cut(s) 477, 611, 630, 633, 690
FriOI GRGCYC 1 cut(s) 565
Fsp4HI GCNGC 5 cut(s) 477, 611, 630, 633, 690
FspBI CTAG 4 cut(s) 150, 221, 345, 396
GlaI GCGC 1 cut(s) 710
GluI GCNGC 5 cut(s) 477, 611, 630, 633, 690
HaeIII GGCC 2 cut(s) 199, 349
HhaI GCGC 1 cut(s) 711
Hin1II CATG 6 cut(s) 163, 215, 327, 485, 627, 848
Hin6I GCGC 1 cut(s) 709
HinP1I GCGC 1 cut(s) 709
HinfI GANTC 7 cut(s) 137, 226, 272, 286, 514, 779, 855
HphI GGTGA 1 cut(s) 62
Hpy188I TCNGA 1 cut(s) 262
Hpy188III TCNNGA 2 cut(s) 212, 845
HpyAV CCTTC 6 cut(s) 210, 426, 637, 759, 828, 834
HpyCH4III ACNGT 2 cut(s) 445, 789
HpyCH4V TGCA 4 cut(s) 323, 665, 685, 692
HpyF10VI GCNNNNNNNGC 5 cut(s) 140, 329, 560, 686, 698
HpyF3I CTNAG 2 cut(s) 110, 261
Hsp92II CATG 6 cut(s) 163, 215, 327, 485, 627, 848
HspAI GCGC 1 cut(s) 709
Kzo9I GATC 3 cut(s) 26, 448, 810
LmnI GCTCC 2 cut(s) 16, 402
LpnPI CCDG 3 cut(s) 361, 625, 691
Lsp1109I GCAGC 5 cut(s) 463, 616, 619, 622, 676
MaeI CTAG 4 cut(s) 150, 221, 345, 396
MaeIII GTNAC 2 cut(s) 216, 734
MalI GATC 3 cut(s) 28, 450, 812
MbiI CCGCTC 1 cut(s) 143
MboI GATC 3 cut(s) 26, 448, 810
MboII GAAGA 2 cut(s) 285, 820
MfeI CAATTG 1 cut(s) 771
MflI RGATCY 1 cut(s) 810
MhlI GDGCHC 1 cut(s) 565
MluCI AATT 4 cut(s) 9, 82, 187, 771
MlyI GAGTC 4 cut(s) 280, 508, 788, 849
MseI TTAA 2 cut(s) 6, 865
MslI CAYNNNNRTG 1 cut(s) 486
MunI CAATTG 1 cut(s) 771
Mva1269I GAATGC 1 cut(s) 334
MvnI CGCG 1 cut(s) 711
MwoI GCNNNNNNNGC 5 cut(s) 140, 329, 560, 686, 698
NcoI CCATGG 2 cut(s) 159, 623
NdeII GATC 3 cut(s) 26, 448, 810
NlaIII CATG 6 cut(s) 163, 215, 327, 485, 627, 848
NlaIV GGNNCC 1 cut(s) 89
NspV TTCGAA 1 cut(s) 296
PagI TCATGA 2 cut(s) 211, 844
PceI AGGCCT 1 cut(s) 349
PctI GAATGC 1 cut(s) 334
PfeI GAWTC 3 cut(s) 137, 226, 272
PkrI GCNGC 5 cut(s) 478, 612, 631, 634, 691
PleI GAGTC 4 cut(s) 280, 508, 787, 849
PpsI GAGTC 4 cut(s) 280, 508, 787, 849
PspN4I GGNNCC 1 cut(s) 89
PspPI GGNCC 1 cut(s) 98
PstI CTGCAG 1 cut(s) 694
PsuI RGATCY 1 cut(s) 810
RsaI GTAC 1 cut(s) 61
RsaNI GTAC 1 cut(s) 60
RseI CAYNNNNRTG 1 cut(s) 486
SaqAI TTAA 2 cut(s) 6, 865
SatI GCNGC 5 cut(s) 477, 611, 630, 633, 690
Sau3AI GATC 3 cut(s) 26, 448, 810
Sau96I GGNCC 1 cut(s) 98
SchI GAGTC 4 cut(s) 280, 508, 788, 849
SduI GDGCHC 1 cut(s) 565
SetI ASST 9 cut(s) 21, 155, 267, 407, 437, 551, 691, 747, 836
SfcI CTRYAG 1 cut(s) 690
SfuI TTCGAA 1 cut(s) 296
SinI GGWCC 1 cut(s) 98
SmiMI CAYNNNNRTG 1 cut(s) 486
Sse9I AATT 4 cut(s) 9, 82, 187, 771
SseBI AGGCCT 1 cut(s) 349
SsiI CCGC 2 cut(s) 141, 724
SspMI CTAG 4 cut(s) 150, 221, 345, 396
StuI AGGCCT 1 cut(s) 349
StyI CCWWGG 3 cut(s) 159, 359, 623
TaaI ACNGT 2 cut(s) 445, 789
TaqI TCGA 3 cut(s) 25, 280, 296
TasI AATT 4 cut(s) 9, 82, 187, 771
TfiI GAWTC 3 cut(s) 137, 226, 272
Tru1I TTAA 2 cut(s) 6, 865
Tru9I TTAA 2 cut(s) 6, 865
TscAI CASTG 1 cut(s) 172
TseI GCWGC 5 cut(s) 476, 610, 629, 632, 689
TspDTI ATGAA 6 cut(s) 225, 397, 498, 607, 833, 861
TspGWI ACGGA 2 cut(s) 326, 722
TspRI CASTG 1 cut(s) 172
VpaK11BI GGWCC 1 cut(s) 98
XspI CTAG 4 cut(s) 150, 221, 345, 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.