Rh3BG372200

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0006991
Physical Location & Seq
Reverse (-)
47009117 .. 47009875
759 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG372200.1

Sequence Viewer

Length: 759 bp
ATGATTAATTTTGGTTTTTGGAATGTTCGTGGTCTCAATGACCCTTCAAAGCATCTGGAAGTGTTAAATCTTGTTTTTATGCATCACCTTGATCTTTTCTGTCTAGTGGAAACGCGTGTAAGGGAGGTAAATGCTGACTCCTTATCAAGATCTATTTTTCCTAATTGGAGCTTTATTAATAACTTTTGCAGTCATCCTAATGGAAGAATTTGGATTTTTTGGAACTCCACCAAAATTTCTCTTGACAAACTGGATGCTTCTAGTCAGGTTGTTCACTGCAAGCTTAGACTTTTAGACCTTCAGGTCGATGTCTTTGCCTCTTTTGTCTATGGTTCGAATGATGACTCAACTAGGAGAGAGCTTTGGCATAGCATGGAAAACTTTGCTAGTGTCTGTAGTAGTTCTCCTTGGTTAGTTATTGGTGACTTTAATGTTACTAGAAGATGTGAGGAAACTGTTGGTGGCAATAATCGTTTTAGTAACGCTATGAATGAGTTCAACTCTTGTCTTCATAATTCTGAGCTTGATGATCTTAACTACTCTGGTATTTACTATTCTTGGAGTAATAAGCAGGTTGATGGTAATTTTATTTCCAGAAAGTTGGACCGTGCCTTGGTTAATCAGTATTGGATGAACTCTTTTCCCAATTGTAGTACTATGTTCTTGCCCCTGGTATTTTTGACCATGCTACTTTGGTGGTTAAATTTGGTTTACCTATCAGAACCGGCAGAGCTCCCTTTAAGTTCTTTAACTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

252

Amino Acids

29.24

Weight (kDa)

5.6

Isoelectric Point (pI)

37.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 7 - 207 4.9e-08 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 302
Acc36I ACCTGC 1 cut(s) 562
AccII CGCG 1 cut(s) 115
AcsI RAATTY 3 cut(s) 207, 234, 703
AcuI CTGAAG 1 cut(s) 284
AfaI GTAC 1 cut(s) 655
AfiI CCNNNNNNNGG 1 cut(s) 613
AflIII ACRYGT 1 cut(s) 113
AgsI TTSAA 2 cut(s) 48, 499
AjnI CCWGG 1 cut(s) 669
AluBI AGCT 5 cut(s) 171, 283, 361, 523, 733
AluI AGCT 5 cut(s) 171, 283, 361, 523, 733
Alw21I GWGCWC 1 cut(s) 735
Alw26I GTCTC 1 cut(s) 38
ApoI RAATTY 3 cut(s) 207, 234, 703
AseI ATTAAT 2 cut(s) 6, 177
Asp700I GAANNNNTTC 1 cut(s) 494
AspS9I GGNCC 1 cut(s) 604
AsuHPI GGTGA 2 cut(s) 77, 434
AsuII TTCGAA 1 cut(s) 335
AvaII GGWCC 1 cut(s) 604
BanII GRGCYC 1 cut(s) 735
BbsI GAAGAC 1 cut(s) 500
Bbv12I GWGCWC 1 cut(s) 735
BccI CCATC 1 cut(s) 572
BcgI CGANNNNNNTGC 2 cut(s) 296, 330
BciT130I CCWGG 1 cut(s) 671
BcoDI GTCTC 1 cut(s) 38
BfaI CTAG 5 cut(s) 104, 261, 351, 387, 438
BfmI CTRYAG 1 cut(s) 394
BfuAI ACCTGC 1 cut(s) 562
BglII AGATCT 1 cut(s) 149
BmcAI AGTACT 1 cut(s) 655
Bme1390I CCNGG 1 cut(s) 671
Bme18I GGWCC 1 cut(s) 604
BmgT120I GGNCC 1 cut(s) 604
BmrFI CCNGG 1 cut(s) 671
BmsI GCATC 3 cut(s) 61, 91, 244
BpiI GAAGAC 1 cut(s) 500
BplI GAGNNNNNCTC 2 cut(s) 485, 517
Bpu14I TTCGAA 1 cut(s) 335
BsaI GGTCTC 1 cut(s) 38
BsaJI CCNNGG 3 cut(s) 407, 612, 669
Bsc4I CCNNNNNNNGG 1 cut(s) 613
Bse118I RCCGGY 1 cut(s) 724
Bse1I ACTGG 1 cut(s) 255
BseBI CCWGG 1 cut(s) 671
BseDI CCNNGG 3 cut(s) 407, 612, 669
BseGI GGATG 3 cut(s) 193, 259, 636
BseLI CCNNNNNNNGG 1 cut(s) 613
BseMII CTCAG 1 cut(s) 510
BseNI ACTGG 1 cut(s) 255
Bsh1236I CGCG 1 cut(s) 115
BsiHKAI GWGCWC 1 cut(s) 735
BsiSI CCGG 1 cut(s) 725
BslI CCNNNNNNNGG 1 cut(s) 613
BsmAI GTCTC 1 cut(s) 38
Bso31I GGTCTC 1 cut(s) 38
Bsp119I TTCGAA 1 cut(s) 335
Bsp1286I GDGCHC 1 cut(s) 735
Bsp143I GATC 3 cut(s) 91, 149, 529
BspCNI CTCAG 1 cut(s) 511
BspFNI CGCG 1 cut(s) 115
BspMI ACCTGC 1 cut(s) 562
BspT104I TTCGAA 1 cut(s) 335
BspTNI GGTCTC 1 cut(s) 38
BsrFI RCCGGY 1 cut(s) 724
BsrI ACTGG 1 cut(s) 255
BssAI RCCGGY 1 cut(s) 724
BssECI CCNNGG 3 cut(s) 407, 612, 669
BssMI GATC 3 cut(s) 91, 149, 529
BssT1I CCWWGG 2 cut(s) 407, 612
Bst2UI CCWGG 1 cut(s) 671
Bst4CI ACNGT 2 cut(s) 457, 608
BstBI TTCGAA 1 cut(s) 335
BstC8I GCNNGC 1 cut(s) 281
BstDEI CTNAG 3 cut(s) 284, 519, 756
BstF5I GGATG 3 cut(s) 193, 259, 636
BstFNI CGCG 1 cut(s) 115
BstKTI GATC 3 cut(s) 94, 152, 532
BstMAI GTCTC 1 cut(s) 38
BstMBI GATC 3 cut(s) 91, 149, 529
BstNI CCWGG 1 cut(s) 671
BstSCI CCNGG 1 cut(s) 669
BstSFI CTRYAG 1 cut(s) 394
BstUI CGCG 1 cut(s) 115
BstV2I GAAGAC 1 cut(s) 500
BstX2I RGATCY 1 cut(s) 149
BstXI CCANNNNNNTGG 1 cut(s) 601
BstYI RGATCY 1 cut(s) 149
BtsCI GGATG 3 cut(s) 193, 259, 636
BtsI GCAGTG 1 cut(s) 274
BtsIMutI CAGTG 1 cut(s) 274
BveI ACCTGC 1 cut(s) 562
Cac8I GCNNGC 1 cut(s) 281
Cfr10I RCCGGY 1 cut(s) 724
Cfr13I GGNCC 1 cut(s) 604
Csp6I GTAC 1 cut(s) 654
CviAII CATG 2 cut(s) 373, 685
CviJI RGCY 5 cut(s) 171, 283, 361, 523, 733
CviKI_1 RGCY 5 cut(s) 171, 283, 361, 523, 733
CviQI GTAC 1 cut(s) 654
DdeI CTNAG 3 cut(s) 284, 519, 756
DpnI GATC 3 cut(s) 93, 151, 531
DpnII GATC 3 cut(s) 91, 149, 529
DrdI GACNNNNNNGTC 1 cut(s) 302
DseDI GACNNNNNNGTC 1 cut(s) 302
Ecl136II GAGCTC 1 cut(s) 733
Eco130I CCWWGG 2 cut(s) 407, 612
Eco24I GRGCYC 1 cut(s) 735
Eco31I GGTCTC 1 cut(s) 38
Eco47I GGWCC 1 cut(s) 604
Eco53kI GAGCTC 1 cut(s) 733
Eco57I CTGAAG 1 cut(s) 284
EcoICRI GAGCTC 1 cut(s) 733
EcoRII CCWGG 1 cut(s) 669
EcoT14I CCWWGG 2 cut(s) 407, 612
EcoT22I ATGCAT 1 cut(s) 84
EcoT38I GRGCYC 1 cut(s) 735
ErhI CCWWGG 2 cut(s) 407, 612
FaeI CATG 2 cut(s) 376, 688
FaiI YATR 8 cut(s) 80, 330, 369, 374, 488, 513, 659, 686
FatI CATG 2 cut(s) 372, 684
FokI GGATG 3 cut(s) 180, 266, 643
FriOI GRGCYC 1 cut(s) 735
FspBI CTAG 5 cut(s) 104, 261, 351, 387, 438
HapII CCGG 1 cut(s) 725
Hin1II CATG 2 cut(s) 376, 688
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 2 cut(s) 137, 344
HpaII CCGG 1 cut(s) 725
HphI GGTGA 2 cut(s) 77, 434
Hpy166II GTNNAC 2 cut(s) 274, 712
Hpy188I TCNGA 2 cut(s) 520, 721
Hpy188III TCNNGA 4 cut(s) 56, 147, 242, 594
Hpy8I GTNNAC 2 cut(s) 274, 712
HpyAV CCTTC 2 cut(s) 54, 308
HpyCH4III ACNGT 2 cut(s) 457, 608
HpyCH4V TGCA 3 cut(s) 82, 189, 279
HpyF3I CTNAG 3 cut(s) 284, 519, 756
Hsp92II CATG 2 cut(s) 376, 688
Kzo9I GATC 3 cut(s) 91, 149, 529
LmnI GCTCC 2 cut(s) 168, 738
LweI GCATC 3 cut(s) 61, 91, 244
MaeI CTAG 5 cut(s) 104, 261, 351, 387, 438
MaeIII GTNAC 3 cut(s) 422, 433, 479
MalI GATC 3 cut(s) 93, 151, 531
MboI GATC 3 cut(s) 91, 149, 529
MboII GAAGA 3 cut(s) 216, 453, 500
MfeI CAATTG 1 cut(s) 646
MflI RGATCY 1 cut(s) 149
MhlI GDGCHC 1 cut(s) 735
MluCI AATT 8 cut(s) 7, 163, 207, 234, 514, 583, 646, 703
MluI ACGCGT 1 cut(s) 113
MlyI GAGTC 2 cut(s) 131, 338
MmeI TCCRAC 1 cut(s) 582
MnlI CCTC 3 cut(s) 118, 328, 442
Mph1103I ATGCAT 1 cut(s) 84
MroXI GAANNNNTTC 1 cut(s) 494
MseI TTAA 9 cut(s) 6, 65, 177, 429, 534, 618, 701, 740, 749
MslI CAYNNNNRTG 1 cut(s) 198
MspI CCGG 1 cut(s) 725
MspR9I CCNGG 1 cut(s) 671
MunI CAATTG 1 cut(s) 646
MvaI CCWGG 1 cut(s) 671
MvnI CGCG 1 cut(s) 115
NdeII GATC 3 cut(s) 91, 149, 529
NlaIII CATG 2 cut(s) 376, 688
NmuCI GTSAC 1 cut(s) 422
NsiI ATGCAT 1 cut(s) 84
NspV TTCGAA 1 cut(s) 335
PdmI GAANNNNTTC 1 cut(s) 494
PleI GAGTC 2 cut(s) 131, 338
PpsI GAGTC 2 cut(s) 131, 338
PshBI ATTAAT 2 cut(s) 6, 177
Psp124BI GAGCTC 1 cut(s) 735
Psp6I CCWGG 1 cut(s) 669
PspGI CCWGG 1 cut(s) 669
PspPI GGNCC 1 cut(s) 604
PsuI RGATCY 1 cut(s) 149
RsaI GTAC 1 cut(s) 655
RsaNI GTAC 1 cut(s) 654
RseI CAYNNNNRTG 1 cut(s) 198
SacI GAGCTC 1 cut(s) 735
SaqAI TTAA 9 cut(s) 6, 65, 177, 429, 534, 618, 701, 740, 749
Sau3AI GATC 3 cut(s) 91, 149, 529
Sau96I GGNCC 1 cut(s) 604
ScaI AGTACT 1 cut(s) 655
SchI GAGTC 2 cut(s) 131, 338
ScrFI CCNGG 1 cut(s) 671
SduI GDGCHC 1 cut(s) 735
SfaNI GCATC 3 cut(s) 61, 91, 244
SfcI CTRYAG 1 cut(s) 394
SfuI TTCGAA 1 cut(s) 335
SinI GGWCC 1 cut(s) 604
SmiMI CAYNNNNRTG 1 cut(s) 198
Sse9I AATT 8 cut(s) 7, 163, 207, 234, 514, 583, 646, 703
SspMI CTAG 5 cut(s) 104, 261, 351, 387, 438
SstI GAGCTC 1 cut(s) 735
StyD4I CCNGG 1 cut(s) 669
StyI CCWWGG 2 cut(s) 407, 612
TaaI ACNGT 2 cut(s) 457, 608
TaqI TCGA 2 cut(s) 306, 335
TasI AATT 8 cut(s) 7, 163, 207, 234, 514, 583, 646, 703
TatI WGTACW 1 cut(s) 653
Tru1I TTAA 9 cut(s) 6, 65, 177, 429, 534, 618, 701, 740, 749
Tru9I TTAA 9 cut(s) 6, 65, 177, 429, 534, 618, 701, 740, 749
TscAI CASTG 1 cut(s) 281
TseFI GTSAC 1 cut(s) 422
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 3 cut(s) 500, 503, 647
TspRI CASTG 1 cut(s) 281
VpaK11BI GGWCC 1 cut(s) 604
VspI ATTAAT 2 cut(s) 6, 177
XapI RAATTY 3 cut(s) 207, 234, 703
XmnI GAANNNNTTC 1 cut(s) 494
XspI CTAG 5 cut(s) 104, 261, 351, 387, 438
ZrmI AGTACT 1 cut(s) 655
Zsp2I ATGCAT 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.