Rmu_sc0005227.1_g000014
ERF Family

Transmembrane 9 superfamily member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005227.1
OG0003988
Physical Location & Seq
Reverse (-)
58103 .. 60220
2118 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1638 bp
atgcgagaggcacagtattgcaaagttgcatgccgtgtaaaactggatgctaattctgcgaaaagttttaaggacaagatcgatggtgaatatcaagttaacatgattcttgatgatctccctgttgcagttgcactcgaaatgagaaactctcgagatgagggtcgatcaaagatatataagcttgggttcatggttgggattaagggacgttatgctggagaaaaagaagaaagacatttcattcacaaccacctgagttttaaagtcatgtatcatgaggatccagagactgattatgctcacattgtcggttttgaggtcacgccgtacagtattaatcacgagtacaaggagtggaatggtgagaagactaagcttgtcacatgccagccgagcaccaatacttacattgaaggtagcaatagcccacaagggattcatactgatgaagaaattgttttcacatatgatgtatcttttgagtccagtgatatcaagtgggaatcgcgttgggactcatacctctacctaggtgatgatccaattcattggtttgctatggtcaattcattgataactgtgctcttcctttccggagtggtagccattgttgtgatgagaactcttttcaaggatattaccaactacaatcagttggagacccaagttgaagaaacaggatggaaactggtccatggagatgtcttcaggccacccatcaatgctaatatattgtgtgtttttgttgcgaatggtgtccaagtctttggtacagtgcttttcacaataactcttgcattgctgggtttcctttcaccttcgaaccaaggtggggtgattaaagcaatggttcttttatggatgttcatggccttatttgggggatactactcggcgcacttgtacaagatgtttaaaggcattcagtggaagaagattgccttaaagacagcctttatgttccctgctacatttttcggaatatttttcatgttgaatgtcttcatttggtgggaggagtcttttgctgtagtgccgtttggtacaatgttttcaatgctgctcttgtggtttgggatatcagtgccattagtatttgtaggcagttacttggggttcaaacagcaagcaattgaggatccagtcaataccaatgaaatcgcaagacaaataccagtgcgagcatggtacaacataaagatggtcttgtctgtgctcatcggaggcattctacagttcggagtagtgttcgttgagttcttctttatgtggacatccatatggatgaataagttctactactctttcgggttcctcttaatagtctttgtcatccttctagtcacctgttgtgagatagcaattctactctgctacttccaacttcgcagcgaagattaccattggtggtggagagcatacttgactgccggctcctctgctctgtacatgtttgcatactccattttccttttcttcaccaggctgtggattgaaaagtttgtttctgtcatcctttattttgggtacatgttaattgcatcctttgcgttctttgtcttgactgggacaatcggcttctatgcatgcttctggtttgttaggaagatctactcctctgtcaagtttgactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP70

Protein Analysis

545

Amino Acids

63.29

Weight (kDa)

6.29

Isoelectric Point (pI)

38.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP70 PF02990 1 - 502 7.7e-174 Endomembrane protein 70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1491
AccII CGCG 1 cut(s) 511
AccIII TCCGGA 1 cut(s) 596
AclWI GGATC 5 cut(s) 278, 291, 536, 1136, 1149
AcuI CTGAAG 1 cut(s) 694
AfaI GTAC 8 cut(s) 332, 350, 775, 908, 1048, 1193, 1451, 1532
AfiI CCNNNNNNNGG 3 cut(s) 835, 882, 1491
AflIII ACRYGT 2 cut(s) 1452, 1533
AgsI TTSAA 7 cut(s) 416, 634, 674, 1000, 1059, 1123, 1499
AjnI CCWGG 1 cut(s) 1484
AluBI AGCT 2 cut(s) 184, 379
AluI AGCT 2 cut(s) 184, 379
Alw21I GWGCWC 3 cut(s) 401, 588, 1221
Alw26I GTCTC 2 cut(s) 284, 656
AlwI GGATC 5 cut(s) 278, 291, 536, 1136, 1149
AlwNI CAGNNNCTG 1 cut(s) 293
Ama87I CYCGRG 1 cut(s) 153
Aor13HI TCCGGA 1 cut(s) 596
AoxI GGCC 2 cut(s) 713, 873
ApeKI GCWGC 2 cut(s) 1063, 1392
AseI ATTAAT 1 cut(s) 339
Asp700I GAANNNNTTC 2 cut(s) 1004, 1295
AspA2I CCTAGG 1 cut(s) 532
AspLEI GCGC 1 cut(s) 901
AspS9I GGNCC 1 cut(s) 694
AsuHPI GGTGA 7 cut(s) 98, 377, 548, 810, 850, 1339, 1474
AsuII TTCGAA 1 cut(s) 824
AvaI CYCGRG 1 cut(s) 153
AvaII GGWCC 1 cut(s) 694
AvrII CCTAGG 1 cut(s) 532
BamHI GGATCC 2 cut(s) 283, 1141
BauI CACGAG 1 cut(s) 344
BbsI GAAGAC 3 cut(s) 377, 700, 997
Bbv12I GWGCWC 3 cut(s) 401, 588, 1221
BbvI GCAGC 2 cut(s) 1050, 1404
BccI CCATC 4 cut(s) 77, 678, 728, 1198
BceAI ACGGC 3 cut(s) 18, 313, 1024
BciT130I CCWGG 1 cut(s) 1486
BciVI GTATCC 1 cut(s) 881
BcoDI GTCTC 2 cut(s) 284, 656
BfaI CTAG 2 cut(s) 533, 1343
BfmI CTRYAG 2 cut(s) 1032, 1235
BfuI GTATCC 1 cut(s) 881
BglII AGATCT 1 cut(s) 1611
BisI GCNGC 2 cut(s) 1064, 1393
BlnI CCTAGG 1 cut(s) 532
BlsI GCNGC 2 cut(s) 1065, 1394
Bme1390I CCNGG 1 cut(s) 1486
Bme18I GGWCC 1 cut(s) 694
BmeT110I CYCGRG 1 cut(s) 153
BmgT120I GGNCC 1 cut(s) 694
BmiI GGNNCC 4 cut(s) 285, 1143, 1316, 1438
BmrFI CCNGG 1 cut(s) 1486
BmrI ACTGGG 1 cut(s) 1578
BmsI GCATC 2 cut(s) 37, 1553
BmuI ACTGGG 1 cut(s) 1578
BpiI GAAGAC 3 cut(s) 377, 700, 997
BplI GAGNNNNNCTC 2 cut(s) 136, 168
BpmI CTGGAG 1 cut(s) 240
Bpu14I TTCGAA 1 cut(s) 824
Bsa29I ATCGAT 1 cut(s) 81
BsaI GGTCTC 1 cut(s) 656
BsaJI CCNNGG 3 cut(s) 532, 697, 829
BsaWI WCCGGW 1 cut(s) 596
Bsc4I CCNNNNNNNGG 3 cut(s) 835, 882, 1491
Bse118I RCCGGY 1 cut(s) 1433
Bse1I ACTGG 6 cut(s) 48, 489, 696, 1145, 1178, 1573
Bse3DI GCAATG 2 cut(s) 800, 855
BseAI TCCGGA 1 cut(s) 596
BseBI CCWGG 1 cut(s) 1486
BseCI ATCGAT 1 cut(s) 81
BseDI CCNNGG 3 cut(s) 532, 697, 829
BseGI GGATG 8 cut(s) 52, 689, 870, 1277, 1293, 1335, 1515, 1544
BseLI CCNNNNNNNGG 3 cut(s) 835, 882, 1491
BseMI GCAATG 2 cut(s) 800, 855
BseMII CTCAG 1 cut(s) 248
BseNI ACTGG 6 cut(s) 48, 489, 696, 1145, 1178, 1573
BseRI GAGGAG 3 cut(s) 1034, 1429, 1609
BseXI GCAGC 2 cut(s) 1050, 1404
BseYI CCCAGC 1 cut(s) 805
Bsh1236I CGCG 1 cut(s) 511
BshFI GGCC 2 cut(s) 715, 875
BshVI ATCGAT 1 cut(s) 81
BsiHKAI GWGCWC 3 cut(s) 401, 588, 1221
BsiHKCI CYCGRG 1 cut(s) 153
BsiSI CCGG 2 cut(s) 597, 1434
BslFI GGGAC 3 cut(s) 222, 530, 1585
BslI CCNNNNNNNGG 3 cut(s) 835, 882, 1491
BsmAI GTCTC 2 cut(s) 284, 656
BsmFI GGGAC 3 cut(s) 222, 530, 1585
BsmI GAATGC 2 cut(s) 924, 1230
BsnI GGCC 2 cut(s) 715, 875
Bso31I GGTCTC 1 cut(s) 656
BsoBI CYCGRG 1 cut(s) 153
Bsp119I TTCGAA 1 cut(s) 824
Bsp1286I GDGCHC 3 cut(s) 401, 588, 1221
Bsp13I TCCGGA 1 cut(s) 596
Bsp1407I TGTACA 2 cut(s) 906, 1449
Bsp143I GATC 7 cut(s) 78, 115, 167, 283, 541, 1141, 1611
Bsp19I CCATGG 1 cut(s) 697
BspANI GGCC 2 cut(s) 715, 875
BspCNI CTCAG 1 cut(s) 249
BspDI ATCGAT 1 cut(s) 81
BspEI TCCGGA 1 cut(s) 596
BspFNI CGCG 1 cut(s) 511
BspHI TCATGA 1 cut(s) 277
BspLI GGNNCC 4 cut(s) 285, 1143, 1316, 1438
BspPI GGATC 5 cut(s) 278, 291, 536, 1136, 1149
BspQI GCTCTTC 1 cut(s) 593
BspT104I TTCGAA 1 cut(s) 824
BspTNI GGTCTC 1 cut(s) 656
BsrDI GCAATG 2 cut(s) 800, 855
BsrFI RCCGGY 1 cut(s) 1433
BsrGI TGTACA 2 cut(s) 906, 1449
BsrI ACTGG 6 cut(s) 48, 489, 696, 1145, 1178, 1573
BssAI RCCGGY 1 cut(s) 1433
BssECI CCNNGG 3 cut(s) 532, 697, 829
BssMI GATC 7 cut(s) 78, 115, 167, 283, 541, 1141, 1611
BssSI CACGAG 1 cut(s) 344
BssT1I CCWWGG 3 cut(s) 532, 697, 829
Bst2BI CACGAG 1 cut(s) 344
Bst2UI CCWGG 1 cut(s) 1486
Bst4CI ACNGT 5 cut(s) 15, 335, 583, 778, 1239
Bst6I CTCTTC 1 cut(s) 593
BstAPI GCANNNNNTGC 1 cut(s) 1550
BstAUI TGTACA 2 cut(s) 906, 1449
BstBI TTCGAA 1 cut(s) 824
BstC8I GCNNGC 6 cut(s) 31, 392, 1131, 1185, 1435, 1591
BstDEI CTNAG 2 cut(s) 257, 375
BstDSI CCRYGG 1 cut(s) 697
BstF5I GGATG 8 cut(s) 52, 689, 870, 1277, 1293, 1335, 1515, 1544
BstFNI CGCG 1 cut(s) 511
BstHHI GCGC 1 cut(s) 901
BstKTI GATC 7 cut(s) 81, 118, 170, 286, 544, 1144, 1614
BstMAI GTCTC 2 cut(s) 284, 656
BstMBI GATC 7 cut(s) 78, 115, 167, 283, 541, 1141, 1611
BstMWI GCNNNNNNNGC 3 cut(s) 56, 396, 1550
BstNI CCWGG 1 cut(s) 1486
BstNSI RCATGY 5 cut(s) 33, 390, 1456, 1537, 1593
BstSCI CCNGG 1 cut(s) 1484
BstSFI CTRYAG 2 cut(s) 1032, 1235
BstUI CGCG 1 cut(s) 511
BstV1I GCAGC 2 cut(s) 1050, 1404
BstV2I GAAGAC 3 cut(s) 377, 700, 997
BstX2I RGATCY 3 cut(s) 283, 1141, 1611
BstXI CCANNNNNNTGG 2 cut(s) 552, 770
BstYI RGATCY 3 cut(s) 283, 1141, 1611
Bsu15I ATCGAT 1 cut(s) 81
BsuI GTATCC 1 cut(s) 881
BsuRI GGCC 2 cut(s) 715, 875
BsuTUI ATCGAT 1 cut(s) 81
BtgI CCRYGG 1 cut(s) 697
BtsCI GGATG 8 cut(s) 52, 689, 870, 1277, 1293, 1335, 1515, 1544
BtsIMutI CAGTG 5 cut(s) 496, 783, 935, 1092, 1185
Cac8I GCNNGC 6 cut(s) 31, 392, 1131, 1185, 1435, 1591
CaiI CAGNNNCTG 1 cut(s) 293
CciI TCATGA 1 cut(s) 277
CfoI GCGC 1 cut(s) 901
Cfr10I RCCGGY 1 cut(s) 1433
Cfr13I GGNCC 1 cut(s) 694
ClaI ATCGAT 1 cut(s) 81
Csp6I GTAC 8 cut(s) 331, 349, 774, 907, 1047, 1192, 1450, 1531
CviQI GTAC 8 cut(s) 331, 349, 774, 907, 1047, 1192, 1450, 1531
DdeI CTNAG 2 cut(s) 257, 375
DpnI GATC 7 cut(s) 80, 117, 169, 285, 543, 1143, 1613
DpnII GATC 7 cut(s) 78, 115, 167, 283, 541, 1141, 1611
DraI TTTAAA 2 cut(s) 265, 919
Eam1104I CTCTTC 1 cut(s) 593
EarI CTCTTC 1 cut(s) 593
Eco130I CCWWGG 3 cut(s) 532, 697, 829
Eco31I GGTCTC 1 cut(s) 656
Eco32I GATATC 2 cut(s) 496, 1083
Eco47I GGWCC 1 cut(s) 694
Eco57I CTGAAG 1 cut(s) 694
Eco88I CYCGRG 1 cut(s) 153
EcoRII CCWGG 1 cut(s) 1484
EcoRV GATATC 2 cut(s) 496, 1083
EcoT14I CCWWGG 3 cut(s) 532, 697, 829
EcoT22I ATGCAT 1 cut(s) 1591
ErhI CCWWGG 3 cut(s) 532, 697, 829
FalI AAGNNNNNCTT 6 cut(s) 929, 961, 941, 973, 1193, 1225
FaqI GGGAC 3 cut(s) 222, 530, 1585
FauNDI CATATG 2 cut(s) 469, 1283
Fnu4HI GCNGC 2 cut(s) 1064, 1393
FokI GGATG 8 cut(s) 59, 696, 877, 1264, 1300, 1322, 1502, 1531
Fsp4HI GCNGC 2 cut(s) 1064, 1393
FspBI CTAG 2 cut(s) 533, 1343
GlaI GCGC 1 cut(s) 900
GluI GCNGC 2 cut(s) 1064, 1393
GsaI CCCAGC 1 cut(s) 809
GsuI CTGGAG 1 cut(s) 240
HaeIII GGCC 2 cut(s) 715, 875
HapII CCGG 2 cut(s) 597, 1434
HhaI GCGC 1 cut(s) 901
Hin6I GCGC 1 cut(s) 899
HinP1I GCGC 1 cut(s) 899
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HindIII AAGCTT 2 cut(s) 182, 377
HinfI GANTC 6 cut(s) 106, 439, 485, 506, 518, 1022
HpaI GTTAAC 1 cut(s) 100
HpaII CCGG 2 cut(s) 597, 1434
HphI GGTGA 7 cut(s) 98, 377, 548, 810, 850, 1339, 1474
Hpy166II GTNNAC 2 cut(s) 100, 1275
Hpy188I TCNGA 3 cut(s) 983, 1226, 1244
Hpy188III TCNNGA 8 cut(s) 110, 153, 155, 278, 287, 344, 597, 1564
Hpy8I GTNNAC 2 cut(s) 100, 1275
HpyAV CCTTC 3 cut(s) 410, 831, 1349
HpyCH4III ACNGT 5 cut(s) 15, 335, 583, 778, 1239
HpyCH4IV ACGT 1 cut(s) 211
HpyCH4V TGCA 8 cut(s) 21, 29, 128, 134, 800, 1460, 1544, 1589
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 396, 1550
HpyF3I CTNAG 2 cut(s) 257, 375
HpySE526I ACGT 1 cut(s) 211
HspAI GCGC 1 cut(s) 899
Kpn2I TCCGGA 1 cut(s) 596
KroI GCCGGC 1 cut(s) 1433
KroNI GCCGGC 1 cut(s) 1435
KspAI GTTAAC 1 cut(s) 100
Kzo9I GATC 7 cut(s) 78, 115, 167, 283, 541, 1141, 1611
LguI GCTCTTC 1 cut(s) 593
LmnI GCTCC 1 cut(s) 1442
Lsp1109I GCAGC 2 cut(s) 1050, 1404
LweI GCATC 2 cut(s) 37, 1553
MaeI CTAG 2 cut(s) 533, 1343
MaeII ACGT 1 cut(s) 211
MaeIII GTNAC 4 cut(s) 322, 382, 1109, 1345
MalI GATC 7 cut(s) 80, 117, 169, 285, 543, 1143, 1613
MboI GATC 7 cut(s) 78, 115, 167, 283, 541, 1141, 1611
MfeI CAATTG 1 cut(s) 1134
MflI RGATCY 3 cut(s) 283, 1141, 1611
MhlI GDGCHC 3 cut(s) 401, 588, 1221
MluCI AATT 7 cut(s) 52, 456, 546, 568, 1134, 1365, 1539
MlyI GAGTC 3 cut(s) 494, 512, 1031
MmeI TCCRAC 2 cut(s) 639, 1408
Mph1103I ATGCAT 1 cut(s) 1591
MroI TCCGGA 1 cut(s) 596
MroNI GCCGGC 1 cut(s) 1433
MroXI GAANNNNTTC 2 cut(s) 1004, 1295
MslI CAYNNNNRTG 6 cut(s) 447, 614, 702, 1202, 1282, 1286
MspI CCGG 2 cut(s) 597, 1434
MspR9I CCNGG 1 cut(s) 1486
MunI CAATTG 1 cut(s) 1134
Mva1269I GAATGC 2 cut(s) 924, 1230
MvaI CCWGG 1 cut(s) 1486
MvnI CGCG 1 cut(s) 511
MwoI GCNNNNNNNGC 3 cut(s) 56, 396, 1550
NaeI GCCGGC 1 cut(s) 1435
NcoI CCATGG 1 cut(s) 697
NdeI CATATG 2 cut(s) 469, 1283
NdeII GATC 7 cut(s) 78, 115, 167, 283, 541, 1141, 1611
NgoMIV GCCGGC 1 cut(s) 1433
NlaIV GGNNCC 4 cut(s) 285, 1143, 1316, 1438
NmeAIII GCCGAG 2 cut(s) 420, 875
NmuCI GTSAC 3 cut(s) 322, 382, 1345
NsiI ATGCAT 1 cut(s) 1591
NspI RCATGY 5 cut(s) 33, 390, 1456, 1537, 1593
NspV TTCGAA 1 cut(s) 824
PaeI GCATGC 2 cut(s) 33, 1593
PaeR7I CTCGAG 1 cut(s) 153
PagI TCATGA 1 cut(s) 277
PciI ACATGT 2 cut(s) 1452, 1533
PciSI GCTCTTC 1 cut(s) 593
PctI GAATGC 2 cut(s) 924, 1230
PdiI GCCGGC 1 cut(s) 1435
PdmI GAANNNNTTC 2 cut(s) 1004, 1295
PfeI GAWTC 3 cut(s) 106, 439, 506
PflMI CCANNNNNTGG 1 cut(s) 1491
PkrI GCNGC 2 cut(s) 1065, 1394
PleI GAGTC 3 cut(s) 493, 512, 1030
PpsI GAGTC 3 cut(s) 493, 512, 1030
PscI ACATGT 2 cut(s) 1452, 1533
PshBI ATTAAT 1 cut(s) 339
Psp6I CCWGG 1 cut(s) 1484
PspFI CCCAGC 1 cut(s) 805
PspGI CCWGG 1 cut(s) 1484
PspN4I GGNNCC 4 cut(s) 285, 1143, 1316, 1438
PspPI GGNCC 1 cut(s) 694
PstNI CAGNNNCTG 1 cut(s) 293
PsuI RGATCY 3 cut(s) 283, 1141, 1611
RsaI GTAC 8 cut(s) 332, 350, 775, 908, 1048, 1193, 1451, 1532
RsaNI GTAC 8 cut(s) 331, 349, 774, 907, 1047, 1192, 1450, 1531
RseI CAYNNNNRTG 6 cut(s) 447, 614, 702, 1202, 1282, 1286
SapI GCTCTTC 1 cut(s) 593
SatI GCNGC 2 cut(s) 1064, 1393
Sau3AI GATC 7 cut(s) 78, 115, 167, 283, 541, 1141, 1611
Sau96I GGNCC 1 cut(s) 694
SchI GAGTC 3 cut(s) 494, 512, 1031
ScrFI CCNGG 1 cut(s) 1486
SduI GDGCHC 3 cut(s) 401, 588, 1221
SfaNI GCATC 2 cut(s) 37, 1553
SfcI CTRYAG 2 cut(s) 1032, 1235
Sfr274I CTCGAG 1 cut(s) 153
SfuI TTCGAA 1 cut(s) 824
SinI GGWCC 1 cut(s) 694
SlaI CTCGAG 1 cut(s) 153
SmiMI CAYNNNNRTG 6 cut(s) 447, 614, 702, 1202, 1282, 1286
SmlI CTYRAG 1 cut(s) 153
SmoI CTYRAG 1 cut(s) 153
SphI GCATGC 2 cut(s) 33, 1593
Sse9I AATT 7 cut(s) 52, 456, 546, 568, 1134, 1365, 1539
SspI AATATT 1 cut(s) 987
SspMI CTAG 2 cut(s) 533, 1343
StyD4I CCNGG 1 cut(s) 1484
StyI CCWWGG 3 cut(s) 532, 697, 829
TaaI ACNGT 5 cut(s) 15, 335, 583, 778, 1239
TaiI ACGT 1 cut(s) 214
TaqI TCGA 5 cut(s) 81, 138, 154, 166, 824
TasI AATT 7 cut(s) 52, 456, 546, 568, 1134, 1365, 1539
TatI WGTACW 3 cut(s) 348, 906, 1449
TfiI GAWTC 3 cut(s) 106, 439, 506
TscAI CASTG 5 cut(s) 496, 783, 935, 1092, 1185
TseFI GTSAC 3 cut(s) 322, 382, 1345
TseI GCWGC 2 cut(s) 1063, 1392
Tsp45I GTSAC 3 cut(s) 322, 382, 1345
TspRI CASTG 5 cut(s) 496, 783, 935, 1092, 1185
Van91I CCANNNNNTGG 1 cut(s) 1491
VpaK11BI GGWCC 1 cut(s) 694
VspI ATTAAT 1 cut(s) 339
XceI RCATGY 5 cut(s) 33, 390, 1456, 1537, 1593
XcmI CCANNNNNNNNNTGG 1 cut(s) 1185
XhoI CTCGAG 1 cut(s) 153
XmaJI CCTAGG 1 cut(s) 532
XmnI GAANNNNTTC 2 cut(s) 1004, 1295
XspI CTAG 2 cut(s) 533, 1343
Zsp2I ATGCAT 1 cut(s) 1591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.