Rmu_sc0005652.1_g000031

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005652.1
OG0007672
Physical Location & Seq
Forward (+)
139451 .. 143184
3734 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1092 bp
atgaggtggcagatcggtaatggtgttggggttcgagtttggatggatccgtggcttccaaatgtctatccttttgttccggtgcctcaggcaattggtgtggatgtcactttgcttgtggagaatcttcttttagctccagggaggtgggatatgaataaggtgcatgcctgtaaactcttcttgggactacaacatgcagctaatggtgggggtgcagcagttatttcaaaggtttggaaaggtgtatggcaagcttgtattcctggaactgctcgaatttgcatatggaaagtgcttcagaacatattgcctacagttgacagattggtgtcaaagggagtacaaattgagtcttatttgtctgtgctgtgtgggatggggaatgagtcctcagatcatttatgtcgtttatgtccttttacgaagactttattggagtcggaggaagagctcaagcaagtttgttatgaggagaggaatcatagagtttgggaacaggtagaggcccgggaaggtgatatgcttttcagcttaaatgctaggcttactgacttccagttctataggaggtcatttcctaggattgagaaggggagttgtaggtggaaacctcctgctcaagaacatgtggaggctcttgctggtaaagcagctgtgaagtatgtacgagataaagggctctctccagtagtgtttgagacggattctttaactctttctaatgctactaagcaagttgtcattccccatacttcccaattgggtcgtgtgtatgatgacataatagaaaagttgaatttcattccaggattctcagaaaacttccttcatgaaagttgtagaacgcgtcgatacgagttcgtggacatgcggaacgcattaatcggagttcatcagttcgcctcctcagtctggtcacccctgtggtgttctctagtggcgatatccaccgtgtgtggcacagcaaggcggagcaggttggtgttttcggacccgaccggaaaacgtagctccggcgaccgtacggcttcaagtttccttccttggcttcgtgggggtcgtctgagtcgatctatggtgtttgtttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
RVT_1 zf-RVT

Protein Analysis

363

Amino Acids

40.94

Weight (kDa)

9.12

Isoelectric Point (pI)

53.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 78 - 144 1.2e-09 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 969
AccB1I GGYRCC 1 cut(s) 82
AccII CGCG 1 cut(s) 850
AciI CCGC 2 cut(s) 874, 973
AclWI GGATC 2 cut(s) 41, 54
AcsI RAATTY 2 cut(s) 279, 799
AcuI CTGAAG 1 cut(s) 284
AdeI CACNNNGTG 1 cut(s) 957
AfaI GTAC 3 cut(s) 345, 669, 1027
AfiI CCNNNNNNNGG 1 cut(s) 915
AflIII ACRYGT 2 cut(s) 628, 848
AgsI TTSAA 3 cut(s) 231, 799, 1035
AjnI CCWGG 3 cut(s) 139, 265, 808
AjuI GAANNNNNNNTTGG 2 cut(s) 419, 451
AleI CACNNNNGTG 1 cut(s) 925
AluBI AGCT 7 cut(s) 137, 203, 257, 454, 534, 656, 1014
AluI AGCT 7 cut(s) 137, 203, 257, 454, 534, 656, 1014
Alw21I GWGCWC 1 cut(s) 456
Alw26I GTCTC 1 cut(s) 695
AlwI GGATC 2 cut(s) 41, 54
Ama87I CYCGRG 1 cut(s) 510
AoxI GGCC 1 cut(s) 507
ApeKI GCWGC 3 cut(s) 200, 218, 653
ApoI RAATTY 2 cut(s) 279, 799
AseI ATTAAT 1 cut(s) 884
AspA2I CCTAGG 1 cut(s) 581
AspS9I GGNCC 2 cut(s) 508, 994
AsuC2I CCSGG 2 cut(s) 511, 512
AsuHPI GGTGA 2 cut(s) 530, 912
AvaI CYCGRG 1 cut(s) 510
AvaII GGWCC 1 cut(s) 994
AvrII CCTAGG 1 cut(s) 581
AxyI CCTNAGG 1 cut(s) 87
BamHI GGATCC 1 cut(s) 46
BanI GGYRCC 1 cut(s) 82
BanII GRGCYC 2 cut(s) 456, 684
BbsI GAAGAC 1 cut(s) 434
Bbv12I GWGCWC 1 cut(s) 456
BbvI GCAGC 3 cut(s) 212, 230, 665
BccI CCATC 2 cut(s) 37, 373
BceAI ACGGC 1 cut(s) 1044
BciT130I CCWGG 3 cut(s) 141, 267, 810
BcnI CCSGG 2 cut(s) 511, 512
BcoDI GTCTC 1 cut(s) 695
BfaI CTAG 3 cut(s) 543, 582, 938
BfmI CTRYAG 2 cut(s) 315, 565
BfuAI ACCTGC 1 cut(s) 969
BisI GCNGC 3 cut(s) 201, 219, 654
BlnI CCTAGG 1 cut(s) 581
BlsI GCNGC 3 cut(s) 202, 220, 655
Bme1390I CCNGG 5 cut(s) 141, 267, 511, 512, 810
Bme18I GGWCC 1 cut(s) 994
BmeT110I CYCGRG 1 cut(s) 510
BmgT120I GGNCC 2 cut(s) 508, 994
BmiI GGNNCC 3 cut(s) 48, 84, 996
BmrFI CCNGG 5 cut(s) 141, 267, 511, 512, 810
BpiI GAAGAC 1 cut(s) 434
BpmI CTGGAG 2 cut(s) 123, 672
BpuEI CTTGAG 2 cut(s) 440, 606
BpuMI CCSGG 2 cut(s) 511, 512
BsaJI CCNNGG 5 cut(s) 50, 140, 510, 581, 1046
BsaWI WCCGGW 2 cut(s) 79, 1001
Bsc4I CCNNNNNNNGG 1 cut(s) 915
Bse1I ACTGG 2 cut(s) 559, 689
Bse21I CCTNAGG 1 cut(s) 87
BseBI CCWGG 3 cut(s) 141, 267, 810
BseDI CCNNGG 5 cut(s) 50, 140, 510, 581, 1046
BseGI GGATG 3 cut(s) 48, 109, 384
BseLI CCNNNNNNNGG 1 cut(s) 915
BseMII CTCAG 5 cut(s) 101, 408, 831, 924, 1058
BseNI ACTGG 2 cut(s) 559, 689
BseRI GAGGAG 2 cut(s) 488, 898
BseXI GCAGC 3 cut(s) 212, 230, 665
BsgI GTGCAG 1 cut(s) 237
Bsh1236I CGCG 1 cut(s) 850
Bsh1285I CGRYCG 2 cut(s) 1002, 1024
BshFI GGCC 1 cut(s) 509
BshNI GGYRCC 1 cut(s) 82
BsiEI CGRYCG 2 cut(s) 1002, 1024
BsiHKAI GWGCWC 1 cut(s) 456
BsiHKCI CYCGRG 1 cut(s) 510
BsiSI CCGG 4 cut(s) 80, 511, 1002, 1017
BsiWI CGTACG 1 cut(s) 1025
BslFI GGGAC 1 cut(s) 201
BslI CCNNNNNNNGG 1 cut(s) 915
BsmAI GTCTC 1 cut(s) 695
BsmBI CGTCTC 1 cut(s) 695
BsmFI GGGAC 1 cut(s) 201
BsnI GGCC 1 cut(s) 509
BsoBI CYCGRG 1 cut(s) 510
Bsp1286I GDGCHC 2 cut(s) 456, 684
Bsp143I GATC 4 cut(s) 12, 46, 397, 1073
BspACI CCGC 2 cut(s) 874, 973
BspANI GGCC 1 cut(s) 509
BspCNI CTCAG 5 cut(s) 100, 407, 830, 923, 1059
BspFNI CGCG 1 cut(s) 850
BspHI TCATGA 1 cut(s) 832
BspLI GGNNCC 3 cut(s) 48, 84, 996
BspMI ACCTGC 1 cut(s) 969
BspPI GGATC 2 cut(s) 41, 54
BspQI GCTCTTC 1 cut(s) 444
BspT107I GGYRCC 1 cut(s) 82
BsrI ACTGG 2 cut(s) 559, 689
BssECI CCNNGG 5 cut(s) 50, 140, 510, 581, 1046
BssMI GATC 4 cut(s) 12, 46, 397, 1073
BssT1I CCWWGG 2 cut(s) 581, 1046
Bst2UI CCWGG 3 cut(s) 141, 267, 810
Bst4CI ACNGT 3 cut(s) 319, 955, 1025
Bst6I CTCTTC 2 cut(s) 185, 444
BstC8I GCNNGC 2 cut(s) 168, 255
BstDEI CTNAG 6 cut(s) 87, 394, 732, 817, 910, 1067
BstDSI CCRYGG 1 cut(s) 50
BstEII GGTNACC 1 cut(s) 918
BstF5I GGATG 3 cut(s) 48, 109, 384
BstFNI CGCG 1 cut(s) 850
BstKTI GATC 4 cut(s) 15, 49, 400, 1076
BstMAI GTCTC 1 cut(s) 695
BstMBI GATC 4 cut(s) 12, 46, 397, 1073
BstMCI CGRYCG 2 cut(s) 1002, 1024
BstMWI GCNNNNNNNGC 1 cut(s) 650
BstNI CCWGG 3 cut(s) 141, 267, 810
BstNSI RCATGY 4 cut(s) 170, 200, 632, 874
BstPI GGTNACC 1 cut(s) 918
BstSCI CCNGG 5 cut(s) 139, 265, 509, 510, 808
BstSFI CTRYAG 2 cut(s) 315, 565
BstUI CGCG 1 cut(s) 850
BstV1I GCAGC 3 cut(s) 212, 230, 665
BstV2I GAAGAC 1 cut(s) 434
BstX2I RGATCY 1 cut(s) 46
BstXI CCANNNNNNTGG 1 cut(s) 147
BstYI RGATCY 1 cut(s) 46
Bsu36I CCTNAGG 1 cut(s) 87
BsuRI GGCC 1 cut(s) 509
BtgI CCRYGG 1 cut(s) 50
BtsCI GGATG 3 cut(s) 48, 109, 384
BveI ACCTGC 1 cut(s) 969
Cac8I GCNNGC 2 cut(s) 168, 255
CciI TCATGA 1 cut(s) 832
Cfr13I GGNCC 2 cut(s) 508, 994
Cfr9I CCCGGG 1 cut(s) 510
CseI GACGC 1 cut(s) 839
Csp6I GTAC 3 cut(s) 344, 668, 1026
CspCI CAANNNNNGTGG 2 cut(s) 81, 116
CviAII CATG 5 cut(s) 167, 197, 629, 833, 871
CviQI GTAC 3 cut(s) 344, 668, 1026
DdeI CTNAG 6 cut(s) 87, 394, 732, 817, 910, 1067
DpnI GATC 4 cut(s) 14, 48, 399, 1075
DpnII GATC 4 cut(s) 12, 46, 397, 1073
DraIII CACNNNGTG 1 cut(s) 957
Eam1104I CTCTTC 2 cut(s) 185, 444
EarI CTCTTC 2 cut(s) 185, 444
EciI GGCGGA 1 cut(s) 988
Ecl136II GAGCTC 1 cut(s) 454
Eco130I CCWWGG 2 cut(s) 581, 1046
Eco24I GRGCYC 2 cut(s) 456, 684
Eco32I GATATC 1 cut(s) 948
Eco47I GGWCC 1 cut(s) 994
Eco53kI GAGCTC 1 cut(s) 454
Eco57I CTGAAG 1 cut(s) 284
Eco81I CCTNAGG 1 cut(s) 87
Eco88I CYCGRG 1 cut(s) 510
Eco91I GGTNACC 1 cut(s) 918
EcoICRI GAGCTC 1 cut(s) 454
EcoO65I GGTNACC 1 cut(s) 918
EcoRII CCWGG 3 cut(s) 139, 265, 808
EcoRV GATATC 1 cut(s) 948
EcoT14I CCWWGG 2 cut(s) 581, 1046
EcoT38I GRGCYC 2 cut(s) 456, 684
ErhI CCWWGG 2 cut(s) 581, 1046
Esp3I CGTCTC 1 cut(s) 695
FaeI CATG 5 cut(s) 170, 200, 632, 836, 874
FaqI GGGAC 1 cut(s) 201
FatI CATG 5 cut(s) 166, 196, 628, 832, 870
FauNDI CATATG 1 cut(s) 287
Fnu4HI GCNGC 3 cut(s) 201, 219, 654
FokI GGATG 3 cut(s) 55, 116, 391
FriOI GRGCYC 2 cut(s) 456, 684
Fsp4HI GCNGC 3 cut(s) 201, 219, 654
FspBI CTAG 3 cut(s) 543, 582, 938
GluI GCNGC 3 cut(s) 201, 219, 654
GsuI CTGGAG 2 cut(s) 123, 672
HaeIII GGCC 1 cut(s) 509
HapII CCGG 4 cut(s) 80, 511, 1002, 1017
HgaI GACGC 1 cut(s) 839
Hin1II CATG 5 cut(s) 170, 200, 632, 836, 874
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HindIII AAGCTT 1 cut(s) 255
HinfI GANTC 8 cut(s) 124, 353, 389, 440, 481, 707, 813, 1069
HpaII CCGG 4 cut(s) 80, 511, 1002, 1017
HphI GGTGA 2 cut(s) 530, 912
Hpy166II GTNNAC 3 cut(s) 176, 322, 868
Hpy188I TCNGA 7 cut(s) 303, 397, 445, 820, 890, 994, 1068
Hpy188III TCNNGA 2 cut(s) 623, 833
Hpy8I GTNNAC 3 cut(s) 176, 322, 868
Hpy99I CGWCG 1 cut(s) 855
HpyAV CCTTC 4 cut(s) 509, 586, 839, 1052
HpyCH4III ACNGT 3 cut(s) 319, 955, 1025
HpyCH4IV ACGT 1 cut(s) 1009
HpyCH4V TGCA 4 cut(s) 166, 200, 218, 285
HpyF10VI GCNNNNNNNGC 1 cut(s) 650
HpyF3I CTNAG 6 cut(s) 87, 394, 732, 817, 910, 1067
HpySE526I ACGT 1 cut(s) 1009
Hsp92II CATG 5 cut(s) 170, 200, 632, 836, 874
Kzo9I GATC 4 cut(s) 12, 46, 397, 1073
LguI GCTCTTC 1 cut(s) 444
LmnI GCTCC 3 cut(s) 142, 975, 1019
Lsp1109I GCAGC 3 cut(s) 212, 230, 665
MaeI CTAG 3 cut(s) 543, 582, 938
MaeII ACGT 1 cut(s) 1009
MaeIII GTNAC 2 cut(s) 106, 918
MalI GATC 4 cut(s) 14, 48, 399, 1075
MboI GATC 4 cut(s) 12, 46, 397, 1073
MboII GAAGA 4 cut(s) 119, 172, 439, 461
MfeI CAATTG 2 cut(s) 93, 761
MflI RGATCY 1 cut(s) 46
MhlI GDGCHC 2 cut(s) 456, 684
MluCI AATT 5 cut(s) 93, 279, 348, 761, 799
MluI ACGCGT 1 cut(s) 848
MlyI GAGTC 4 cut(s) 362, 398, 449, 1078
MmeI TCCRAC 1 cut(s) 423
MseI TTAA 3 cut(s) 536, 713, 884
MslI CAYNNNNRTG 1 cut(s) 925
MspA1I CMGCKG 1 cut(s) 656
MspI CCGG 4 cut(s) 80, 511, 1002, 1017
MspR9I CCNGG 5 cut(s) 141, 267, 511, 512, 810
MunI CAATTG 2 cut(s) 93, 761
MvaI CCWGG 3 cut(s) 141, 267, 810
MvnI CGCG 1 cut(s) 850
MwoI GCNNNNNNNGC 1 cut(s) 650
NciI CCSGG 2 cut(s) 511, 512
NdeI CATATG 1 cut(s) 287
NdeII GATC 4 cut(s) 12, 46, 397, 1073
NlaIII CATG 5 cut(s) 170, 200, 632, 836, 874
NlaIV GGNNCC 3 cut(s) 48, 84, 996
NmuCI GTSAC 2 cut(s) 106, 918
NspI RCATGY 4 cut(s) 170, 200, 632, 874
OliI CACNNNNGTG 1 cut(s) 925
PaeI GCATGC 1 cut(s) 170
PagI TCATGA 1 cut(s) 832
PciI ACATGT 1 cut(s) 628
PciSI GCTCTTC 1 cut(s) 444
PcsI WCGNNNNNNNCGW 2 cut(s) 1060, 1069
PfeI GAWTC 4 cut(s) 124, 481, 707, 813
Pfl23II CGTACG 1 cut(s) 1025
PfoI TCCNGGA 2 cut(s) 265, 808
PkrI GCNGC 3 cut(s) 202, 220, 655
PleI GAGTC 4 cut(s) 361, 397, 448, 1077
PpsI GAGTC 4 cut(s) 361, 397, 448, 1077
PscI ACATGT 1 cut(s) 628
PshBI ATTAAT 1 cut(s) 884
Psp124BI GAGCTC 1 cut(s) 456
Psp6I CCWGG 3 cut(s) 139, 265, 808
PspEI GGTNACC 1 cut(s) 918
PspGI CCWGG 3 cut(s) 139, 265, 808
PspLI CGTACG 1 cut(s) 1025
PspN4I GGNNCC 3 cut(s) 48, 84, 996
PspPI GGNCC 2 cut(s) 508, 994
PsuI RGATCY 1 cut(s) 46
PvuII CAGCTG 1 cut(s) 656
RsaI GTAC 3 cut(s) 345, 669, 1027
RsaNI GTAC 3 cut(s) 344, 668, 1026
RseI CAYNNNNRTG 1 cut(s) 925
SacI GAGCTC 1 cut(s) 456
SapI GCTCTTC 1 cut(s) 444
SaqAI TTAA 3 cut(s) 536, 713, 884
SatI GCNGC 3 cut(s) 201, 219, 654
Sau3AI GATC 4 cut(s) 12, 46, 397, 1073
Sau96I GGNCC 2 cut(s) 508, 994
SchI GAGTC 4 cut(s) 362, 398, 449, 1078
ScrFI CCNGG 5 cut(s) 141, 267, 511, 512, 810
SduI GDGCHC 2 cut(s) 456, 684
SfcI CTRYAG 2 cut(s) 315, 565
SinI GGWCC 1 cut(s) 994
SmaI CCCGGG 1 cut(s) 512
SmiMI CAYNNNNRTG 1 cut(s) 925
SmlI CTYRAG 2 cut(s) 455, 621
SmoI CTYRAG 2 cut(s) 455, 621
SphI GCATGC 1 cut(s) 170
Sse9I AATT 5 cut(s) 93, 279, 348, 761, 799
SsiI CCGC 2 cut(s) 874, 973
SspMI CTAG 3 cut(s) 543, 582, 938
SstI GAGCTC 1 cut(s) 456
StyD4I CCNGG 5 cut(s) 139, 265, 509, 510, 808
StyI CCWWGG 2 cut(s) 581, 1046
TaaI ACNGT 3 cut(s) 319, 955, 1025
TaiI ACGT 1 cut(s) 1012
TaqI TCGA 4 cut(s) 34, 277, 853, 1072
TasI AATT 5 cut(s) 93, 279, 348, 761, 799
TatI WGTACW 1 cut(s) 343
TfiI GAWTC 4 cut(s) 124, 481, 707, 813
Tru1I TTAA 3 cut(s) 536, 713, 884
Tru9I TTAA 3 cut(s) 536, 713, 884
TseFI GTSAC 2 cut(s) 106, 918
TseI GCWGC 3 cut(s) 200, 218, 653
Tsp45I GTSAC 2 cut(s) 106, 918
TspDTI ATGAA 5 cut(s) 170, 793, 821, 849, 884
TspGWI ACGGA 2 cut(s) 39, 719
TspMI CCCGGG 1 cut(s) 510
VpaK11BI GGWCC 1 cut(s) 994
VspI ATTAAT 1 cut(s) 884
XapI RAATTY 2 cut(s) 279, 799
XceI RCATGY 4 cut(s) 170, 200, 632, 874
XmaI CCCGGG 1 cut(s) 510
XmaJI CCTAGG 1 cut(s) 581
XspI CTAG 3 cut(s) 543, 582, 938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.