Rmu_sc0005887.1_g000006

Uncharacterised ACR, YagE family COG1723

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005887.1
OG0005702
Physical Location & Seq
Reverse (-)
26702 .. 29426
2725 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1122 bp
atggggagatggagagcagcttctcttctcctcaggaacatagccataaccaccacaccacccaaatcttcttctcttcttttcaagcctctcactctctcacgctcttcttcaatccccacttcccaattctacatcttcctcaagtctaggcctctctctgcaatcccatctcgggtctcaattgacccgactgatttcgaatccgggccggcctattgcgctcatgaacaacccaatgaagacgacgaaaccgggaaaatccccattaaggcgtatttcctttgcaccagtatcaatttgaagagcatgcaggccgagtatttaagcaatgtcattcctccttcgtctcgctcaaccaattatattgcactcagattttgtgatttcccttcggaaaatagtagatttggagtatgggagaatccaagctcctggcgctacatggtagtattccagtatggatcaactgttctgtttaatgttgaggagcatgaagttcaggcttacctaaatctagtcataagacatgcttctggaatgctacgagagatggtgaaagacgattatgctgtaaaagagaagcctcagctggtcgacgatatgcagggtgggccagactacatagttcttagaactttggacacggatagtatccgcattattggaagtgtgcttggccaaagtattgctttagattattttgtttcgcaggttgatggtatggttgaagagtttgcagatataaatcgaggaatggaaaaaactggaacttttaccatgcataaaaagaagctccttcaactcgttggaaaggctaactcaactctagctgatgtaattgtcaaagttggtctatttgagagatcagaaattgcttggagggatgcaaagtatgctaaaatacatgaatacctgagggaggagtatgaggttacgcagcgatttggaaacttggatttcaagttaaaatttgtagagcacaacattcatttcttgcaagaagtcctacaaaacagaaagtctgatcttctggaatggtgcatcatcttcctgttgactgtagagaatattatatcactatacgagattgttcgtgattcaggtgcagtttctgggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF155

Protein Analysis

373

Amino Acids

42.5

Weight (kDa)

5.89

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF155 PF02582 149 - 319 7.1e-33 RMND1/Sif2-Sif3/Mrx10, DUF155
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 703
AccI GTMKAC 1 cut(s) 597
AciI CCGC 1 cut(s) 658
AclWI GGATC 1 cut(s) 472
AcoI YGGCCR 1 cut(s) 679
AcsI RAATTY 1 cut(s) 971
AfiI CCNNNNNNNGG 3 cut(s) 175, 271, 922
AgsI TTSAA 6 cut(s) 85, 114, 304, 731, 803, 964
AjnI CCWGG 1 cut(s) 434
AloI GAACNNNNNNTCC 2 cut(s) 456, 488
AluBI AGCT 5 cut(s) 20, 432, 592, 796, 833
AluI AGCT 5 cut(s) 20, 432, 592, 796, 833
Alw21I GWGCWC 1 cut(s) 984
Alw26I GTCTC 2 cut(s) 184, 354
AlwI GGATC 1 cut(s) 472
AlwNI CAGNNNCTG 1 cut(s) 1115
Ama87I CYCGRG 1 cut(s) 174
AoxI GGCC 6 cut(s) 152, 209, 213, 315, 614, 679
ApeKI GCWGC 2 cut(s) 17, 940
ApoI RAATTY 1 cut(s) 971
AspLEI GCGC 2 cut(s) 224, 441
AspS9I GGNCC 2 cut(s) 209, 614
AsuC2I CCSGG 2 cut(s) 208, 256
AsuHPI GGTGA 1 cut(s) 568
AsuII TTCGAA 1 cut(s) 201
AvaI CYCGRG 1 cut(s) 174
AxyI CCTNAGG 2 cut(s) 32, 917
BalI TGGCCA 1 cut(s) 681
BbsI GAAGAC 1 cut(s) 249
Bbv12I GWGCWC 1 cut(s) 984
BbvCI CCTCAGC 1 cut(s) 588
BbvI GCAGC 2 cut(s) 29, 952
BccI CCATC 4 cut(s) 3, 178, 547, 713
BciT130I CCWGG 1 cut(s) 436
BciVI GTATCC 1 cut(s) 665
BcnI CCSGG 2 cut(s) 208, 256
BcoDI GTCTC 2 cut(s) 184, 354
BfaI CTAG 3 cut(s) 150, 518, 830
BfmI CTRYAG 1 cut(s) 1062
BfoI RGCGCY 1 cut(s) 442
BfuAI ACCTGC 1 cut(s) 703
BfuI GTATCC 1 cut(s) 665
BisI GCNGC 2 cut(s) 18, 941
BlsI GCNGC 2 cut(s) 19, 942
Bme1390I CCNGG 3 cut(s) 208, 256, 436
BmeT110I CYCGRG 1 cut(s) 174
BmgT120I GGNCC 2 cut(s) 209, 614
BmrFI CCNGG 3 cut(s) 208, 256, 436
BmsI GCATC 2 cut(s) 877, 1053
BpiI GAAGAC 1 cut(s) 249
Bpu10I CCTNAGC 1 cut(s) 588
Bpu14I TTCGAA 1 cut(s) 201
BpuEI CTTGAG 1 cut(s) 128
BpuMI CCSGG 2 cut(s) 208, 256
BsaBI GATNNNNATC 1 cut(s) 747
BsaI GGTCTC 1 cut(s) 184
Bsc4I CCNNNNNNNGG 3 cut(s) 175, 271, 922
Bse118I RCCGGY 1 cut(s) 211
Bse1I ACTGG 3 cut(s) 291, 457, 772
Bse21I CCTNAGG 2 cut(s) 32, 917
Bse3DI GCAATG 1 cut(s) 337
Bse8I GATNNNNATC 1 cut(s) 747
BseBI CCWGG 1 cut(s) 436
BseGI GGATG 1 cut(s) 892
BseJI GATNNNNATC 1 cut(s) 747
BseLI CCNNNNNNNGG 3 cut(s) 175, 271, 922
BseMI GCAATG 1 cut(s) 337
BseMII CTCAG 4 cut(s) 46, 388, 602, 908
BseNI ACTGG 3 cut(s) 291, 457, 772
BseRI GAGGAG 3 cut(s) 20, 503, 938
BseXI GCAGC 2 cut(s) 29, 952
BshFI GGCC 6 cut(s) 154, 211, 215, 317, 616, 681
BsiHKAI GWGCWC 1 cut(s) 984
BsiHKCI CYCGRG 1 cut(s) 174
BsiSI CCGG 3 cut(s) 207, 212, 255
BslI CCNNNNNNNGG 3 cut(s) 175, 271, 922
BsmAI GTCTC 2 cut(s) 184, 354
BsmBI CGTCTC 1 cut(s) 354
BsmI GAATGC 1 cut(s) 546
BsnI GGCC 6 cut(s) 154, 211, 215, 317, 616, 681
Bso31I GGTCTC 1 cut(s) 184
BsoBI CYCGRG 1 cut(s) 174
Bsp119I TTCGAA 1 cut(s) 201
Bsp1286I GDGCHC 1 cut(s) 984
Bsp143I GATC 3 cut(s) 464, 866, 1027
BspACI CCGC 1 cut(s) 658
BspANI GGCC 6 cut(s) 154, 211, 215, 317, 616, 681
BspCNI CTCAG 4 cut(s) 45, 387, 601, 909
BspHI TCATGA 1 cut(s) 226
BspMI ACCTGC 1 cut(s) 703
BspPI GGATC 1 cut(s) 472
BspQI GCTCTTC 2 cut(s) 112, 299
BspT104I TTCGAA 1 cut(s) 201
BspTNI GGTCTC 1 cut(s) 184
BsrDI GCAATG 1 cut(s) 337
BsrFI RCCGGY 1 cut(s) 211
BsrI ACTGG 3 cut(s) 291, 457, 772
BssAI RCCGGY 1 cut(s) 211
BssMI GATC 3 cut(s) 464, 866, 1027
Bst2UI CCWGG 1 cut(s) 436
Bst4CI ACNGT 2 cut(s) 472, 1063
Bst6I CTCTTC 5 cut(s) 30, 81, 112, 299, 726
BstAPI GCANNNNNTGC 1 cut(s) 896
BstBI TTCGAA 1 cut(s) 201
BstC8I GCNNGC 3 cut(s) 213, 311, 315
BstDEI CTNAG 5 cut(s) 32, 374, 588, 632, 917
BstENI CCTNNNNNAGG 1 cut(s) 920
BstF5I GGATG 1 cut(s) 892
BstH2I RGCGCY 1 cut(s) 442
BstHHI GCGC 2 cut(s) 224, 441
BstKTI GATC 3 cut(s) 467, 869, 1030
BstMAI GTCTC 2 cut(s) 184, 354
BstMBI GATC 3 cut(s) 464, 866, 1027
BstMWI GCNNNNNNNGC 4 cut(s) 221, 438, 613, 896
BstNI CCWGG 1 cut(s) 436
BstNSI RCATGY 2 cut(s) 313, 533
BstSCI CCNGG 3 cut(s) 206, 254, 434
BstSFI CTRYAG 1 cut(s) 1062
BstV1I GCAGC 2 cut(s) 29, 952
BstV2I GAAGAC 1 cut(s) 249
BstXI CCANNNNNNTGG 1 cut(s) 435
Bsu36I CCTNAGG 2 cut(s) 32, 917
BsuI GTATCC 1 cut(s) 665
BsuRI GGCC 6 cut(s) 154, 211, 215, 317, 616, 681
BtsCI GGATG 1 cut(s) 892
BveI ACCTGC 1 cut(s) 703
Cac8I GCNNGC 3 cut(s) 213, 311, 315
CaiI CAGNNNCTG 1 cut(s) 1115
CciI TCATGA 1 cut(s) 226
CfoI GCGC 2 cut(s) 224, 441
Cfr10I RCCGGY 1 cut(s) 211
Cfr13I GGNCC 2 cut(s) 209, 614
CviAII CATG 7 cut(s) 227, 310, 445, 494, 530, 781, 908
DdeI CTNAG 5 cut(s) 32, 374, 588, 632, 917
DpnI GATC 3 cut(s) 466, 868, 1029
DpnII GATC 3 cut(s) 464, 866, 1027
EaeI YGGCCR 1 cut(s) 679
Eam1104I CTCTTC 5 cut(s) 30, 81, 112, 299, 726
EarI CTCTTC 5 cut(s) 30, 81, 112, 299, 726
Eco147I AGGCCT 1 cut(s) 154
Eco31I GGTCTC 1 cut(s) 184
Eco81I CCTNAGG 2 cut(s) 32, 917
Eco88I CYCGRG 1 cut(s) 174
EcoNI CCTNNNNNAGG 1 cut(s) 920
EcoRII CCWGG 1 cut(s) 434
EcoT22I ATGCAT 1 cut(s) 786
Esp3I CGTCTC 1 cut(s) 354
FaeI CATG 7 cut(s) 230, 313, 448, 497, 533, 784, 911
FalI AAGNNNNNCTT 4 cut(s) 517, 549, 676, 708
FatI CATG 7 cut(s) 226, 309, 444, 493, 529, 780, 907
FblI GTMKAC 1 cut(s) 597
Fnu4HI GCNGC 2 cut(s) 18, 941
FokI GGATG 1 cut(s) 899
FseI GGCCGGCC 1 cut(s) 215
Fsp4HI GCNGC 2 cut(s) 18, 941
FspBI CTAG 3 cut(s) 150, 518, 830
GlaI GCGC 2 cut(s) 223, 440
GluI GCNGC 2 cut(s) 18, 941
HaeII RGCGCY 1 cut(s) 442
HaeIII GGCC 6 cut(s) 154, 211, 215, 317, 616, 681
HapII CCGG 3 cut(s) 207, 212, 255
HhaI GCGC 2 cut(s) 224, 441
Hin1II CATG 7 cut(s) 230, 313, 448, 497, 533, 784, 911
Hin6I GCGC 2 cut(s) 222, 439
HinP1I GCGC 2 cut(s) 222, 439
HincII GTYRAC 2 cut(s) 598, 1059
HindII GTYRAC 2 cut(s) 598, 1059
HinfI GANTC 3 cut(s) 203, 424, 1100
HpaII CCGG 3 cut(s) 207, 212, 255
HphI GGTGA 1 cut(s) 568
Hpy166II GTNNAC 2 cut(s) 598, 1059
Hpy188I TCNGA 4 cut(s) 377, 397, 871, 1027
Hpy188III TCNNGA 5 cut(s) 34, 227, 537, 1034, 1097
Hpy8I GTNNAC 2 cut(s) 598, 1059
Hpy99I CGWCG 2 cut(s) 251, 602
HpyAV CCTTC 3 cut(s) 354, 402, 809
HpyCH4III ACNGT 2 cut(s) 472, 1063
HpyF10VI GCNNNNNNNGC 4 cut(s) 221, 438, 613, 896
HpyF3I CTNAG 5 cut(s) 32, 374, 588, 632, 917
Hsp92II CATG 7 cut(s) 230, 313, 448, 497, 533, 784, 911
HspAI GCGC 2 cut(s) 222, 439
KroI GCCGGC 1 cut(s) 211
KroNI GCCGGC 1 cut(s) 213
Kzo9I GATC 3 cut(s) 464, 866, 1027
LguI GCTCTTC 2 cut(s) 112, 299
LmnI GCTCC 3 cut(s) 437, 490, 801
Lsp1109I GCAGC 2 cut(s) 29, 952
LweI GCATC 2 cut(s) 877, 1053
MaeI CTAG 3 cut(s) 150, 518, 830
MaeIII GTNAC 1 cut(s) 934
MalI GATC 3 cut(s) 466, 868, 1029
MboI GATC 3 cut(s) 464, 866, 1027
MfeI CAATTG 1 cut(s) 183
MhlI GDGCHC 1 cut(s) 984
MlsI TGGCCA 1 cut(s) 681
MluCI AATT 7 cut(s) 128, 183, 298, 361, 840, 873, 971
MluNI TGGCCA 1 cut(s) 681
MmeI TCCRAC 1 cut(s) 790
Mox20I TGGCCA 1 cut(s) 681
Mph1103I ATGCAT 1 cut(s) 786
MroNI GCCGGC 1 cut(s) 211
MscI TGGCCA 1 cut(s) 681
MseI TTAA 4 cut(s) 270, 326, 480, 968
Msp20I TGGCCA 1 cut(s) 681
MspA1I CMGCKG 1 cut(s) 592
MspI CCGG 3 cut(s) 207, 212, 255
MspR9I CCNGG 3 cut(s) 208, 256, 436
MunI CAATTG 1 cut(s) 183
Mva1269I GAATGC 1 cut(s) 546
MvaI CCWGG 1 cut(s) 436
MwoI GCNNNNNNNGC 4 cut(s) 221, 438, 613, 896
NaeI GCCGGC 1 cut(s) 213
NciI CCSGG 2 cut(s) 208, 256
NdeII GATC 3 cut(s) 464, 866, 1027
NgoMIV GCCGGC 1 cut(s) 211
NlaIII CATG 7 cut(s) 230, 313, 448, 497, 533, 784, 911
NmeAIII GCCGAG 1 cut(s) 343
NsiI ATGCAT 1 cut(s) 786
NspI RCATGY 2 cut(s) 313, 533
NspV TTCGAA 1 cut(s) 201
PaeI GCATGC 1 cut(s) 313
PagI TCATGA 1 cut(s) 226
PceI AGGCCT 1 cut(s) 154
PciSI GCTCTTC 2 cut(s) 112, 299
PctI GAATGC 1 cut(s) 546
PdiI GCCGGC 1 cut(s) 213
PfeI GAWTC 3 cut(s) 203, 424, 1100
PkrI GCNGC 2 cut(s) 19, 942
Psp6I CCWGG 1 cut(s) 434
PspGI CCWGG 1 cut(s) 434
PspPI GGNCC 2 cut(s) 209, 614
PstNI CAGNNNCTG 1 cut(s) 1115
PvuII CAGCTG 1 cut(s) 592
RigI GGCCGGCC 1 cut(s) 215
SalI GTCGAC 1 cut(s) 596
SapI GCTCTTC 2 cut(s) 112, 299
SaqAI TTAA 4 cut(s) 270, 326, 480, 968
SatI GCNGC 2 cut(s) 18, 941
Sau3AI GATC 3 cut(s) 464, 866, 1027
Sau96I GGNCC 2 cut(s) 209, 614
ScrFI CCNGG 3 cut(s) 208, 256, 436
SduI GDGCHC 1 cut(s) 984
SfaNI GCATC 2 cut(s) 877, 1053
SfcI CTRYAG 1 cut(s) 1062
SfuI TTCGAA 1 cut(s) 201
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
SphI GCATGC 1 cut(s) 313
Sse9I AATT 7 cut(s) 128, 183, 298, 361, 840, 873, 971
SseBI AGGCCT 1 cut(s) 154
SsiI CCGC 1 cut(s) 658
SspI AATATT 1 cut(s) 1072
SspMI CTAG 3 cut(s) 150, 518, 830
StuI AGGCCT 1 cut(s) 154
StyD4I CCNGG 3 cut(s) 206, 254, 434
TaaI ACNGT 2 cut(s) 472, 1063
TaqI TCGA 3 cut(s) 201, 597, 751
TasI AATT 7 cut(s) 128, 183, 298, 361, 840, 873, 971
TfiI GAWTC 3 cut(s) 203, 424, 1100
Tru1I TTAA 4 cut(s) 270, 326, 480, 968
Tru9I TTAA 4 cut(s) 270, 326, 480, 968
TseI GCWGC 2 cut(s) 17, 940
TspDTI ATGAA 5 cut(s) 243, 255, 510, 924, 980
TspGWI ACGGA 1 cut(s) 662
XagI CCTNNNNNAGG 1 cut(s) 920
XapI RAATTY 1 cut(s) 971
XceI RCATGY 2 cut(s) 313, 533
XmiI GTMKAC 1 cut(s) 597
XspI CTAG 3 cut(s) 150, 518, 830
Zsp2I ATGCAT 1 cut(s) 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.