Rmu_sc0005903.1_g000007

ubiquitin-like-specific protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005903.1
OG0005644
Physical Location & Seq
Reverse (-)
26106 .. 28113
2008 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 930 bp
atgggaaggagaaaacctagcagcgacggacccattaacattgactccgactccgaacccgagtccgacctgaagtgctcgatttcagcagcttcgaaagctcaaagacagagcttgactcggggagaaactgaagagatcaaaaagatatccccatgctttttaactttttctcctcaccgccagagatcaacgaggtgtgttaagagcaagtatgtcaaccgtgaagtgtcaagaaaaacacagaaccaagacactaaaacgtgttcagtgtcaagaagaaaaccagtgccagtctctcgagcttgtagagtgtcacggaagaaacagaagctaggctctgaggcctttgagtgctactttgagaacttatggaagagcttctctgaggacaagaaaacttcctgcacataccttgactgtttatggttttccttgtacattaaaccaaccaccacagacaaggtgctaacctggattaagaagaagcatattttttcaaagaaatatgtttttgttcctattgtgtgctggagccactggaacctactaatattgtgtcattttggtgagaatctcgaatcaaagacccaaagaccatgcatgttgttgcttgattctcttgaaatggcagatccgaggaggctcgaaccaaatataagaaaatttgtagtggacatctttagagaagagggtaggcctgagaatatgaatcttctgagaaaaattcctcttttgatacctaaggtgccacagcagagaaatgatgaggaatgtggtaattttgttctctacttcattaatttgttcacggagagtgctccgcagaattttagcatggagggctacccttactttatgaagaaaaactggtttacatatgaaagcttggattgcttctgccaggaactctattcctcagcaaagtga

Protein Analysis

309

Amino Acids

36.25

Weight (kDa)

9.25

Isoelectric Point (pI)

57.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C48 PF02902 154 - 288 3.4e-11 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 748
AciI CCGC 2 cut(s) 181, 824
AclWI GGATC 1 cut(s) 629
AcsI RAATTY 3 cut(s) 665, 726, 829
AcuI CTGAAG 2 cut(s) 92, 153
AfaI GTAC 1 cut(s) 440
AflIII ACRYGT 1 cut(s) 263
AgsI TTSAA 2 cut(s) 501, 626
AjnI CCWGG 2 cut(s) 473, 903
AluBI AGCT 7 cut(s) 92, 101, 114, 305, 334, 381, 888
AluI AGCT 7 cut(s) 92, 101, 114, 305, 334, 381, 888
Alw21I GWGCWC 2 cut(s) 80, 823
Alw26I GTCTC 1 cut(s) 301
AlwI GGATC 1 cut(s) 629
Ama87I CYCGRG 3 cut(s) 59, 120, 300
AoxI GGCC 2 cut(s) 345, 698
ApeKI GCWGC 2 cut(s) 21, 89
ApoI RAATTY 3 cut(s) 665, 726, 829
AseI ATTAAT 1 cut(s) 801
Asp700I GAANNNNTTC 1 cut(s) 380
AspS9I GGNCC 1 cut(s) 29
AsuHPI GGTGA 2 cut(s) 170, 581
AsuII TTCGAA 1 cut(s) 95
AvaI CYCGRG 3 cut(s) 59, 120, 300
AvaII GGWCC 1 cut(s) 29
AxyI CCTNAGG 1 cut(s) 744
BanI GGYRCC 1 cut(s) 748
Bbv12I GWGCWC 2 cut(s) 80, 823
BbvCI CCTCAGC 1 cut(s) 919
BbvI GCAGC 2 cut(s) 33, 101
BciT130I CCWGG 2 cut(s) 475, 905
BcoDI GTCTC 1 cut(s) 301
BfaI CTAG 2 cut(s) 18, 335
BisI GCNGC 2 cut(s) 22, 90
BlsI GCNGC 2 cut(s) 23, 91
Bme1390I CCNGG 2 cut(s) 475, 905
Bme18I GGWCC 1 cut(s) 29
BmeT110I CYCGRG 3 cut(s) 59, 120, 300
BmgT120I GGNCC 1 cut(s) 29
BmiI GGNNCC 4 cut(s) 31, 536, 545, 750
BmrFI CCNGG 2 cut(s) 475, 905
BplI GAGNNNNNCTC 2 cut(s) 103, 135
BpmI CTGGAG 1 cut(s) 553
Bpu10I CCTNAGC 1 cut(s) 919
Bpu14I TTCGAA 1 cut(s) 95
BsaJI CCNNGG 1 cut(s) 638
BsaXI ACNNNNNCTCC 6 cut(s) 29, 35, 59, 65, 157, 187
Bse1I ACTGG 4 cut(s) 287, 293, 545, 875
Bse21I CCTNAGG 1 cut(s) 744
BseBI CCWGG 2 cut(s) 475, 905
BseDI CCNNGG 1 cut(s) 638
BseMII CTCAG 4 cut(s) 333, 378, 693, 710
BseNI ACTGG 4 cut(s) 287, 293, 545, 875
BseRI GAGGAG 2 cut(s) 165, 655
BseXI GCAGC 2 cut(s) 33, 101
BsgI GTGCAG 1 cut(s) 391
BshFI GGCC 2 cut(s) 347, 700
BshNI GGYRCC 1 cut(s) 748
BsiHKAI GWGCWC 2 cut(s) 80, 823
BsiHKCI CYCGRG 3 cut(s) 59, 120, 300
BsmAI GTCTC 1 cut(s) 301
BsnI GGCC 2 cut(s) 347, 700
BsoBI CYCGRG 3 cut(s) 59, 120, 300
Bsp119I TTCGAA 1 cut(s) 95
Bsp1286I GDGCHC 2 cut(s) 80, 823
Bsp1407I TGTACA 1 cut(s) 438
Bsp143I GATC 3 cut(s) 138, 188, 634
BspACI CCGC 2 cut(s) 181, 824
BspANI GGCC 2 cut(s) 347, 700
BspCNI CTCAG 4 cut(s) 334, 379, 694, 711
BspLI GGNNCC 4 cut(s) 31, 536, 545, 750
BspPI GGATC 1 cut(s) 629
BspQI GCTCTTC 1 cut(s) 371
BspT104I TTCGAA 1 cut(s) 95
BspT107I GGYRCC 1 cut(s) 748
BsrGI TGTACA 1 cut(s) 438
BsrI ACTGG 4 cut(s) 287, 293, 545, 875
BssECI CCNNGG 1 cut(s) 638
BssMI GATC 3 cut(s) 138, 188, 634
Bst2UI CCWGG 2 cut(s) 475, 905
Bst4CI ACNGT 2 cut(s) 224, 422
Bst6I CTCTTC 3 cut(s) 129, 371, 684
BstAUI TGTACA 1 cut(s) 438
BstBI TTCGAA 1 cut(s) 95
BstDEI CTNAG 6 cut(s) 342, 387, 702, 719, 744, 919
BstKTI GATC 3 cut(s) 141, 191, 637
BstMAI GTCTC 1 cut(s) 301
BstMBI GATC 3 cut(s) 138, 188, 634
BstMWI GCNNNNNNNGC 3 cut(s) 98, 843, 894
BstNI CCWGG 2 cut(s) 475, 905
BstNSI RCATGY 1 cut(s) 607
BstSCI CCNGG 2 cut(s) 473, 903
BstV1I GCAGC 2 cut(s) 33, 101
BstX2I RGATCY 1 cut(s) 634
BstYI RGATCY 1 cut(s) 634
Bsu36I CCTNAGG 1 cut(s) 744
BsuRI GGCC 2 cut(s) 347, 700
BtsIMutI CAGTG 3 cut(s) 276, 294, 538
Cfr13I GGNCC 1 cut(s) 29
Csp6I GTAC 1 cut(s) 439
CviAII CATG 4 cut(s) 156, 600, 604, 838
CviQI GTAC 1 cut(s) 439
DdeI CTNAG 6 cut(s) 342, 387, 702, 719, 744, 919
DpnI GATC 3 cut(s) 140, 190, 636
DpnII GATC 3 cut(s) 138, 188, 634
Eam1104I CTCTTC 3 cut(s) 129, 371, 684
EarI CTCTTC 3 cut(s) 129, 371, 684
Eco147I AGGCCT 2 cut(s) 347, 700
Eco32I GATATC 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 29
Eco57I CTGAAG 2 cut(s) 92, 153
Eco81I CCTNAGG 1 cut(s) 744
Eco88I CYCGRG 3 cut(s) 59, 120, 300
EcoRII CCWGG 2 cut(s) 473, 903
EcoRV GATATC 1 cut(s) 150
EcoT22I ATGCAT 1 cut(s) 605
FaeI CATG 4 cut(s) 159, 603, 607, 841
FatI CATG 4 cut(s) 155, 599, 603, 837
FauNDI CATATG 1 cut(s) 880
Fnu4HI GCNGC 2 cut(s) 22, 90
Fsp4HI GCNGC 2 cut(s) 22, 90
FspBI CTAG 2 cut(s) 18, 335
GluI GCNGC 2 cut(s) 22, 90
GsuI CTGGAG 1 cut(s) 553
HaeIII GGCC 2 cut(s) 347, 700
Hin1II CATG 4 cut(s) 159, 603, 607, 841
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HindIII AAGCTT 1 cut(s) 886
HinfI GANTC 8 cut(s) 44, 50, 62, 118, 574, 581, 617, 712
HphI GGTGA 2 cut(s) 170, 581
Hpy166II GTNNAC 4 cut(s) 220, 676, 810, 876
Hpy188I TCNGA 7 cut(s) 49, 55, 67, 343, 388, 639, 720
Hpy188III TCNNGA 5 cut(s) 234, 276, 300, 578, 623
Hpy8I GTNNAC 4 cut(s) 220, 676, 810, 876
Hpy99I CGWCG 1 cut(s) 29
HpyCH4III ACNGT 2 cut(s) 224, 422
HpyCH4IV ACGT 1 cut(s) 263
HpyCH4V TGCA 2 cut(s) 408, 603
HpyF10VI GCNNNNNNNGC 3 cut(s) 98, 843, 894
HpyF3I CTNAG 6 cut(s) 342, 387, 702, 719, 744, 919
HpySE526I ACGT 1 cut(s) 263
Hsp92II CATG 4 cut(s) 159, 603, 607, 841
Kzo9I GATC 3 cut(s) 138, 188, 634
LguI GCTCTTC 1 cut(s) 371
LmnI GCTCC 2 cut(s) 534, 826
Lsp1109I GCAGC 2 cut(s) 33, 101
MaeI CTAG 2 cut(s) 18, 335
MaeII ACGT 1 cut(s) 263
MaeIII GTNAC 1 cut(s) 315
MalI GATC 3 cut(s) 140, 190, 636
MboI GATC 3 cut(s) 138, 188, 634
MboII GAAGA 8 cut(s) 146, 291, 334, 388, 496, 701, 707, 874
MflI RGATCY 1 cut(s) 634
MhlI GDGCHC 2 cut(s) 80, 823
MluCI AATT 5 cut(s) 665, 726, 781, 802, 829
MlyI GAGTC 4 cut(s) 38, 44, 71, 112
MmeI TCCRAC 2 cut(s) 72, 90
Mph1103I ATGCAT 1 cut(s) 605
MroXI GAANNNNTTC 1 cut(s) 380
MseI TTAA 6 cut(s) 36, 164, 204, 444, 480, 801
MslI CAYNNNNRTG 1 cut(s) 567
MspR9I CCNGG 2 cut(s) 475, 905
MvaI CCWGG 2 cut(s) 475, 905
MwoI GCNNNNNNNGC 3 cut(s) 98, 843, 894
NdeI CATATG 1 cut(s) 880
NdeII GATC 3 cut(s) 138, 188, 634
NlaIII CATG 4 cut(s) 159, 603, 607, 841
NlaIV GGNNCC 4 cut(s) 31, 536, 545, 750
NmuCI GTSAC 1 cut(s) 315
NsiI ATGCAT 1 cut(s) 605
NspI RCATGY 1 cut(s) 607
NspV TTCGAA 1 cut(s) 95
PaeR7I CTCGAG 1 cut(s) 300
PceI AGGCCT 2 cut(s) 347, 700
PciSI GCTCTTC 1 cut(s) 371
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 4 cut(s) 574, 581, 617, 712
PkrI GCNGC 2 cut(s) 23, 91
PleI GAGTC 4 cut(s) 38, 44, 70, 112
PpsI GAGTC 4 cut(s) 38, 44, 70, 112
PshBI ATTAAT 1 cut(s) 801
Psp6I CCWGG 2 cut(s) 473, 903
PspGI CCWGG 2 cut(s) 473, 903
PspN4I GGNNCC 4 cut(s) 31, 536, 545, 750
PspPI GGNCC 1 cut(s) 29
PsuI RGATCY 1 cut(s) 634
RsaI GTAC 1 cut(s) 440
RsaNI GTAC 1 cut(s) 439
RseI CAYNNNNRTG 1 cut(s) 567
SapI GCTCTTC 1 cut(s) 371
SaqAI TTAA 6 cut(s) 36, 164, 204, 444, 480, 801
SatI GCNGC 2 cut(s) 22, 90
Sau3AI GATC 3 cut(s) 138, 188, 634
Sau96I GGNCC 1 cut(s) 29
SchI GAGTC 4 cut(s) 38, 44, 71, 112
ScrFI CCNGG 2 cut(s) 475, 905
SduI GDGCHC 2 cut(s) 80, 823
Sfr274I CTCGAG 1 cut(s) 300
SfuI TTCGAA 1 cut(s) 95
SinI GGWCC 1 cut(s) 29
SlaI CTCGAG 1 cut(s) 300
SmiMI CAYNNNNRTG 1 cut(s) 567
SmlI CTYRAG 1 cut(s) 300
SmoI CTYRAG 1 cut(s) 300
Sse9I AATT 5 cut(s) 665, 726, 781, 802, 829
SseBI AGGCCT 2 cut(s) 347, 700
SsiI CCGC 2 cut(s) 181, 824
SspI AATATT 1 cut(s) 555
SspMI CTAG 2 cut(s) 18, 335
StuI AGGCCT 2 cut(s) 347, 700
StyD4I CCNGG 2 cut(s) 473, 903
TaaI ACNGT 2 cut(s) 224, 422
TaiI ACGT 1 cut(s) 266
TaqI TCGA 5 cut(s) 80, 95, 301, 579, 648
TasI AATT 5 cut(s) 665, 726, 781, 802, 829
TatI WGTACW 1 cut(s) 438
TfiI GAWTC 4 cut(s) 574, 581, 617, 712
Tru1I TTAA 6 cut(s) 36, 164, 204, 444, 480, 801
Tru9I TTAA 6 cut(s) 36, 164, 204, 444, 480, 801
TscAI CASTG 3 cut(s) 276, 294, 545
TseFI GTSAC 1 cut(s) 315
TseI GCWGC 2 cut(s) 21, 89
Tsp45I GTSAC 1 cut(s) 315
TspDTI ATGAA 4 cut(s) 725, 787, 875, 897
TspGWI ACGGA 3 cut(s) 42, 334, 827
TspRI CASTG 3 cut(s) 276, 294, 545
VpaK11BI GGWCC 1 cut(s) 29
VspI ATTAAT 1 cut(s) 801
XapI RAATTY 3 cut(s) 665, 726, 829
XceI RCATGY 1 cut(s) 607
XhoI CTCGAG 1 cut(s) 300
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 2 cut(s) 18, 335
Zsp2I ATGCAT 1 cut(s) 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.