Rmu_sc0006207.1_g000009

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006207.1
OG0006042
Physical Location & Seq
Forward (+)
45770 .. 48687
2918 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 861 bp
ctgtctttgtccgcccaccaagatcaatctctatccttctctccattccacctaaaggtgaggaatgcaaccttaagttatatcaaaaccacaaagccgtccagctttgttagtcagaagagggagggtgctggtccgcggagagttgttggggaggtgtatttcgacactggcattatgggcgctgtctgttgttgtttgaatgctgatgattttgaagattatgtgaatccaaacagttctgcattgaggaactgtccatgtcttggttgctttgttcaaaactttgtaaatgtgtattcatcactgttcagaagaggggaactacagtcgctcccctcatccattcagggggctgcatcgatgacttctgcagcctcacttgatagctctttatctgacatgtatcgctctcctccaaggcccttgccttatgatgctgaccccagatacatccgtttgcagcgggatggattggtttctaggcgtgagaaaggcactagtcattctcatgaggagactgaacctctaagaagtgatactgatgcagattcagaatgtttgagtacaggagacaaatggaatggatctacttgtgaagatggatcaaaagaacatcattccaggtcctcgatgaagttgtcatctgcaaaagcaacaaccggatttgggatcatttatacatcttcagaagaagaggatgtatgcccaacatgtcttgaagaatatactccagaaaaccccaaaataatgacaaaatgctctcatcattatcatctcgcttgtatatatgagtggatggagagaagtgaaagttgtcctgtttgtggcaaggtcatggcattcgatgaaacgacttaa

Protein Analysis

286

Amino Acids

31.7

Weight (kDa)

5.51

Isoelectric Point (pI)

60.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 231 - 276 2.2e-10 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 234 - 283 1e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-RING_2 PF13639 235 - 276 5.5e-11 Ring finger domain
zf-C3HC4_2 PF13923 236 - 276 2.6e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 236 - 276 2.2e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 236 - 274 1.6e-06 RING-type zinc-finger
zf-RING_5 PF14634 236 - 278 5.1e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 266
AccII CGCG 1 cut(s) 139
AciI CCGC 4 cut(s) 12, 137, 139, 466
AclWI GGATC 3 cut(s) 595, 613, 680
AcuI CTGAAG 1 cut(s) 672
AfaI GTAC 1 cut(s) 568
AfiI CCNNNNNNNGG 5 cut(s) 55, 266, 351, 669, 827
AflII CTTAAG 1 cut(s) 73
AflIII ACRYGT 2 cut(s) 402, 713
AgsI TTSAA 4 cut(s) 202, 218, 281, 722
AhlI ACTAGT 1 cut(s) 500
AjnI CCWGG 1 cut(s) 623
AluBI AGCT 2 cut(s) 105, 390
AluI AGCT 2 cut(s) 105, 390
Alw26I GTCTC 2 cut(s) 512, 567
AlwI GGATC 3 cut(s) 595, 613, 680
AoxI GGCC 1 cut(s) 422
ApeKI GCWGC 3 cut(s) 356, 374, 463
AspLEI GCGC 1 cut(s) 185
AspS9I GGNCC 3 cut(s) 134, 423, 627
AsuHPI GGTGA 1 cut(s) 70
AvaII GGWCC 2 cut(s) 134, 627
BaeI ACNNNNGTAYC 2 cut(s) 442, 475
BbvI GCAGC 3 cut(s) 343, 386, 475
BccI CCATC 3 cut(s) 464, 596, 793
BceAI ACGGC 1 cut(s) 82
BciT130I CCWGG 1 cut(s) 625
BcoDI GTCTC 2 cut(s) 512, 567
BcuI ACTAGT 1 cut(s) 500
BfaI CTAG 2 cut(s) 483, 501
BfmI CTRYAG 2 cut(s) 326, 372
BfoI RGCGCY 1 cut(s) 186
BfrI CTTAAG 1 cut(s) 73
BisI GCNGC 3 cut(s) 357, 375, 464
BlsI GCNGC 3 cut(s) 358, 376, 465
Bme1390I CCNGG 1 cut(s) 625
Bme18I GGWCC 2 cut(s) 134, 627
BmgT120I GGNCC 3 cut(s) 134, 423, 627
BmrFI CCNGG 1 cut(s) 625
BmsI GCATC 3 cut(s) 368, 427, 535
BpmI CTGGAG 1 cut(s) 717
Bsa29I ATCGAT 1 cut(s) 362
BsaJI CCNNGG 2 cut(s) 137, 419
BsaWI WCCGGW 1 cut(s) 662
BsaXI ACNNNNNCTCC 2 cut(s) 318, 348
Bsc4I CCNNNNNNNGG 5 cut(s) 55, 266, 351, 669, 827
Bse1I ACTGG 1 cut(s) 175
BseBI CCWGG 1 cut(s) 625
BseCI ATCGAT 1 cut(s) 362
BseDI CCNNGG 2 cut(s) 137, 419
BseGI GGATG 5 cut(s) 341, 453, 475, 706, 804
BseLI CCNNNNNNNGG 5 cut(s) 55, 266, 351, 669, 827
BseNI ACTGG 1 cut(s) 175
BseRI GAGGAG 2 cut(s) 405, 530
BseXI GCAGC 3 cut(s) 343, 386, 475
Bsh1236I CGCG 1 cut(s) 139
BshFI GGCC 1 cut(s) 424
BshVI ATCGAT 1 cut(s) 362
BsiSI CCGG 1 cut(s) 663
BslI CCNNNNNNNGG 5 cut(s) 55, 266, 351, 669, 827
BsmAI GTCTC 2 cut(s) 512, 567
BsmI GAATGC 3 cut(s) 70, 208, 842
BsnI GGCC 1 cut(s) 424
Bsp143I GATC 4 cut(s) 22, 587, 605, 672
BspACI CCGC 4 cut(s) 12, 137, 139, 466
BspANI GGCC 1 cut(s) 424
BspDI ATCGAT 1 cut(s) 362
BspFNI CGCG 1 cut(s) 139
BspHI TCATGA 1 cut(s) 511
BspMAI CTGCAG 1 cut(s) 376
BspPI GGATC 3 cut(s) 595, 613, 680
BspTI CTTAAG 1 cut(s) 73
BsrI ACTGG 1 cut(s) 175
BssECI CCNNGG 2 cut(s) 137, 419
BssMI GATC 4 cut(s) 22, 587, 605, 672
BssT1I CCWWGG 1 cut(s) 419
Bst2UI CCWGG 1 cut(s) 625
Bst4CI ACNGT 4 cut(s) 239, 257, 309, 330
Bst6I CTCTTC 3 cut(s) 113, 310, 690
BstAFI CTTAAG 1 cut(s) 73
BstDEI CTNAG 1 cut(s) 530
BstDSI CCRYGG 1 cut(s) 137
BstF5I GGATG 5 cut(s) 341, 453, 475, 706, 804
BstFNI CGCG 1 cut(s) 139
BstH2I RGCGCY 1 cut(s) 186
BstHHI GCGC 1 cut(s) 185
BstKTI GATC 4 cut(s) 25, 590, 608, 675
BstMAI GTCTC 2 cut(s) 512, 567
BstMBI GATC 4 cut(s) 22, 587, 605, 672
BstMWI GCNNNNNNNGC 1 cut(s) 180
BstNI CCWGG 1 cut(s) 625
BstNSI RCATGY 2 cut(s) 406, 717
BstSCI CCNGG 1 cut(s) 623
BstSFI CTRYAG 2 cut(s) 326, 372
BstUI CGCG 1 cut(s) 139
BstV1I GCAGC 3 cut(s) 343, 386, 475
BstX2I RGATCY 1 cut(s) 587
BstYI RGATCY 1 cut(s) 587
Bsu15I ATCGAT 1 cut(s) 362
BsuRI GGCC 1 cut(s) 424
BsuTUI ATCGAT 1 cut(s) 362
BtgI CCRYGG 1 cut(s) 137
BtsCI GGATG 5 cut(s) 341, 453, 475, 706, 804
BtsIMutI CAGTG 2 cut(s) 168, 305
CciI TCATGA 1 cut(s) 511
CfoI GCGC 1 cut(s) 185
Cfr13I GGNCC 3 cut(s) 134, 423, 627
Cfr42I CCGCGG 1 cut(s) 140
ClaI ATCGAT 1 cut(s) 362
Csp6I GTAC 1 cut(s) 567
CviAII CATG 5 cut(s) 261, 403, 512, 714, 838
CviJI RGCY 6 cut(s) 97, 105, 356, 377, 390, 424
CviKI_1 RGCY 6 cut(s) 97, 105, 356, 377, 390, 424
CviQI GTAC 1 cut(s) 567
DdeI CTNAG 1 cut(s) 530
DpnI GATC 4 cut(s) 24, 589, 607, 674
DpnII GATC 4 cut(s) 22, 587, 605, 672
Eam1104I CTCTTC 3 cut(s) 113, 310, 690
EarI CTCTTC 3 cut(s) 113, 310, 690
Eco130I CCWWGG 1 cut(s) 419
Eco47I GGWCC 2 cut(s) 134, 627
Eco57I CTGAAG 1 cut(s) 672
EcoO109I RGGNCCY 2 cut(s) 423, 627
EcoRII CCWGG 1 cut(s) 623
EcoT14I CCWWGG 1 cut(s) 419
ErhI CCWWGG 1 cut(s) 419
FaeI CATG 5 cut(s) 264, 406, 515, 717, 841
FatI CATG 5 cut(s) 260, 402, 511, 713, 837
FauI CCCGC 1 cut(s) 459
Fnu4HI GCNGC 3 cut(s) 357, 375, 464
FokI GGATG 5 cut(s) 328, 440, 482, 713, 811
Fsp4HI GCNGC 3 cut(s) 357, 375, 464
FspBI CTAG 2 cut(s) 483, 501
GlaI GCGC 1 cut(s) 184
GluI GCNGC 3 cut(s) 357, 375, 464
GsuI CTGGAG 1 cut(s) 717
HaeII RGCGCY 1 cut(s) 186
HaeIII GGCC 1 cut(s) 424
HapII CCGG 1 cut(s) 663
HhaI GCGC 1 cut(s) 185
Hin1II CATG 5 cut(s) 264, 406, 515, 717, 841
Hin6I GCGC 1 cut(s) 183
HinP1I GCGC 1 cut(s) 183
HinfI GANTC 2 cut(s) 229, 551
HpaII CCGG 1 cut(s) 663
HphI GGTGA 1 cut(s) 70
Hpy188I TCNGA 5 cut(s) 117, 314, 400, 556, 691
Hpy188III TCNNGA 3 cut(s) 512, 719, 734
HpyAV CCTTC 1 cut(s) 46
HpyCH4III ACNGT 4 cut(s) 239, 257, 309, 330
HpyCH4V TGCA 7 cut(s) 68, 245, 359, 374, 463, 548, 650
HpyF10VI GCNNNNNNNGC 1 cut(s) 180
HpyF3I CTNAG 1 cut(s) 530
Hsp92II CATG 5 cut(s) 264, 406, 515, 717, 841
HspAI GCGC 1 cut(s) 183
KspI CCGCGG 1 cut(s) 140
Kzo9I GATC 4 cut(s) 22, 587, 605, 672
LmnI GCTCC 1 cut(s) 339
Lsp1109I GCAGC 3 cut(s) 343, 386, 475
LweI GCATC 3 cut(s) 368, 427, 535
MaeI CTAG 2 cut(s) 483, 501
MalI GATC 4 cut(s) 24, 589, 607, 674
MboI GATC 4 cut(s) 22, 587, 605, 672
MboII GAAGA 8 cut(s) 130, 230, 327, 611, 678, 704, 707, 734
MflI RGATCY 1 cut(s) 587
MseI TTAA 2 cut(s) 74, 859
MslI CAYNNNNRTG 1 cut(s) 510
MspA1I CMGCKG 2 cut(s) 139, 466
MspCI CTTAAG 1 cut(s) 73
MspI CCGG 1 cut(s) 663
MspR9I CCNGG 1 cut(s) 625
Mva1269I GAATGC 3 cut(s) 70, 208, 842
MvaI CCWGG 1 cut(s) 625
MvnI CGCG 1 cut(s) 139
MwoI GCNNNNNNNGC 1 cut(s) 180
NdeII GATC 4 cut(s) 22, 587, 605, 672
NlaIII CATG 5 cut(s) 264, 406, 515, 717, 841
NspI RCATGY 2 cut(s) 406, 717
PagI TCATGA 1 cut(s) 511
PciI ACATGT 2 cut(s) 402, 713
PctI GAATGC 3 cut(s) 70, 208, 842
PfeI GAWTC 2 cut(s) 229, 551
PflMI CCANNNNNTGG 1 cut(s) 266
PkrI GCNGC 3 cut(s) 358, 376, 465
PpuMI RGGWCCY 1 cut(s) 627
PscI ACATGT 2 cut(s) 402, 713
Psp5II RGGWCCY 1 cut(s) 627
Psp6I CCWGG 1 cut(s) 623
PspGI CCWGG 1 cut(s) 623
PspPI GGNCC 3 cut(s) 134, 423, 627
PspPPI RGGWCCY 1 cut(s) 627
PstI CTGCAG 1 cut(s) 376
PsuI RGATCY 1 cut(s) 587
RsaI GTAC 1 cut(s) 568
RsaNI GTAC 1 cut(s) 567
RseI CAYNNNNRTG 1 cut(s) 510
SacII CCGCGG 1 cut(s) 140
SaqAI TTAA 2 cut(s) 74, 859
SatI GCNGC 3 cut(s) 357, 375, 464
Sau3AI GATC 4 cut(s) 22, 587, 605, 672
Sau96I GGNCC 3 cut(s) 134, 423, 627
ScrFI CCNGG 1 cut(s) 625
SetI ASST 9 cut(s) 54, 60, 74, 107, 159, 392, 529, 629, 837
SfaNI GCATC 3 cut(s) 368, 427, 535
SfcI CTRYAG 2 cut(s) 326, 372
Sfr303I CCGCGG 1 cut(s) 140
SgrBI CCGCGG 1 cut(s) 140
SinI GGWCC 2 cut(s) 134, 627
SmiMI CAYNNNNRTG 1 cut(s) 510
SmlI CTYRAG 1 cut(s) 73
SmoI CTYRAG 1 cut(s) 73
SpeI ACTAGT 1 cut(s) 500
SsiI CCGC 4 cut(s) 12, 137, 139, 466
SspMI CTAG 2 cut(s) 483, 501
StyD4I CCNGG 1 cut(s) 623
StyI CCWWGG 1 cut(s) 419
TaaI ACNGT 4 cut(s) 239, 257, 309, 330
TaqI TCGA 4 cut(s) 165, 362, 632, 846
TatI WGTACW 1 cut(s) 566
TfiI GAWTC 2 cut(s) 229, 551
Tru1I TTAA 2 cut(s) 74, 859
Tru9I TTAA 2 cut(s) 74, 859
TscAI CASTG 2 cut(s) 175, 312
TseI GCWGC 3 cut(s) 356, 374, 463
TspDTI ATGAA 2 cut(s) 291, 650
TspGWI ACGGA 1 cut(s) 446
TspRI CASTG 2 cut(s) 175, 312
Van91I CCANNNNNTGG 1 cut(s) 266
Vha464I CTTAAG 1 cut(s) 73
VpaK11BI GGWCC 2 cut(s) 134, 627
XceI RCATGY 2 cut(s) 406, 717
XspI CTAG 2 cut(s) 483, 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.