Rmu_ssc0000264.1_g000034

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000264.1
OG0013015
Physical Location & Seq
Reverse (-)
196546 .. 197901
1356 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1356 bp
atggaaacaagccgagattgtgaccttgacaagggcaaggaacgtgattttttcttggagagttgtcaatggaagccggtttttgatgaagcttcaatgtcacatagacctcttaagaagatcaagagccctgaacatagtcatcagcaccaatcctcctctgcttttccccctgaatttcatatcccatcttctcctcctcgtagttcatcagatacatcatcaatcacttcttgttgtcctttttcttccagaatagtctttccttttgcttttgatgggtccctgcctcagcagcagcaacctatacaatttccccaccaattccagaccacaggtctgcagcctatacaattcccgccaactacaaatgttgtagagcagcagcagcagcaacaacaaaatcagcagcagatgatatctttcacatcgcagcagcaacaaatgatatcttggcggcagcagcagcaacagcaaatgcttcagtattggagtgatgctttgaatttgagtccaagaggaaggatgatgatgatgaacaggtttggaggagctaatagtagtggtggtattaggccagcggcgatgcctgtaagtactagtaagctttacaggggagtaaggcaaaggcattggggcaaatgggtagctgaaattcgtcttcctcgaaacaggactcgcctctggctgggcaccttcgacaccgctgaagatgctgccatggcttatgaccgtgaggctttcaagttgagaggagagaatgctaagcttaatttccctgagcttttcctcaacaaagacaaagataataataatgctgcagctgtttccacagctccaagttcaaccgaaactgcttctgtgccgactcaacaagtttcaaagccatccaggcaccggggtaagccgctactgaaagaccttgatttacaattacaggttccaatttcagatagggaaacaattgcacctccaccagcgccatattcacatccccaggaagacgatgacactggtgaaagtgatttggccaatgccagccagcaatctgaggttgaaaatcatggcgaaggaggagggatcatttcagatgccccaacacaggaaatggcttggggagaaatggcagaggcttggatgaatgcaattccacaaggttggggtcctggtagtcctgtctgggatgatttagagttggacatgacttccaacaatcttgtgctgcaatctcatgattatcaccattttacggccaataatccgaatcggcatgaacttccgcagcaggaagacaactcttgttctgcttcatcttcatatcccatgcagcccttcttttggaaagatcaagattga

Protein Analysis

451

Amino Acids

51.02

Weight (kDa)

5.47

Isoelectric Point (pI)

62.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 203 - 252 1.8e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013015)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20880 AT4G28140
fragaria_vesca FvH4_6g26090
malus_domestica MD09G1252000.v1.1 MD17G1244300.v1.1
prunus_persica Prupe.3G157100_v2.0.a1
pyrus_communis pycom09g16830 pycom17g24710
rosa_chinensis RchiOBHm_Chr2g0126301
rosa_laevigata RLG00000018898
rosa_multiflora Rmu_ssc0000264.1_g000034
rosa_roxburghii Rroxscaffold_2G00117280
rosa_rugosa Rorug02G0262400
rosa_samantha Rh2AG319300 Rh2BG327700 Rh2CG307700 Rh2DG347300
rosa_wichuraiana Rw2G025870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 692, 894
AciI CCGC 6 cut(s) 359, 457, 581, 705, 908, 1280
AclWI GGATC 1 cut(s) 1088
AcoI YGGCCR 2 cut(s) 1029, 1251
AcsI RAATTY 3 cut(s) 176, 505, 654
AcuI CTGAAG 2 cut(s) 467, 729
AfaI GTAC 1 cut(s) 598
AfiI CCNNNNNNNGG 6 cut(s) 31, 688, 897, 1102, 1249, 1338
AflII CTTAAG 1 cut(s) 113
AgsI TTSAA 6 cut(s) 96, 505, 745, 846, 882, 1058
AhdI GACNNNNNGTC 1 cut(s) 336
AhlI ACTAGT 1 cut(s) 599
AjnI CCWGG 3 cut(s) 890, 996, 1165
AluBI AGCT 8 cut(s) 92, 554, 607, 650, 769, 784, 824, 836
AluI AGCT 8 cut(s) 92, 554, 607, 650, 769, 784, 824, 836
AlwI GGATC 1 cut(s) 1088
AoxI GGCC 3 cut(s) 575, 1029, 1251
ApoI RAATTY 3 cut(s) 176, 505, 654
AspLEI GCGC 1 cut(s) 982
AspS9I GGNCC 2 cut(s) 282, 1163
AsuC2I CCSGG 1 cut(s) 899
AsuHPI GGTGA 2 cut(s) 1028, 1232
AvaII GGWCC 2 cut(s) 282, 1163
BaeGI GKGCMC 1 cut(s) 695
BalI TGGCCA 1 cut(s) 1031
BanI GGYRCC 2 cut(s) 692, 894
BanII GRGCYC 1 cut(s) 131
BbsI GAAGAC 3 cut(s) 653, 1008, 1296
BbvCI CCTCAGC 1 cut(s) 291
BccI CCATC 3 cut(s) 196, 272, 895
BceAI ACGGC 1 cut(s) 1266
BciT130I CCWGG 3 cut(s) 892, 998, 1167
BcnI CCSGG 1 cut(s) 899
BcuI ACTAGT 1 cut(s) 599
BfaI CTAG 1 cut(s) 600
BfmI CTRYAG 2 cut(s) 341, 819
BfoI RGCGCY 1 cut(s) 983
BfrI CTTAAG 1 cut(s) 113
BglI GCCNNNNNGGC 1 cut(s) 892
BlpI GCTNAGC 1 cut(s) 765
BmcAI AGTACT 1 cut(s) 598
Bme1390I CCNGG 4 cut(s) 892, 899, 998, 1167
Bme18I GGWCC 2 cut(s) 282, 1163
BmeRI GACNNNNNGTC 1 cut(s) 336
BmgT120I GGNCC 2 cut(s) 282, 1163
BmiI GGNNCC 6 cut(s) 283, 284, 694, 896, 942, 1164
BmrFI CCNGG 4 cut(s) 892, 899, 998, 1167
BmsI GCATC 4 cut(s) 487, 576, 703, 1081
BpiI GAAGAC 3 cut(s) 653, 1008, 1296
Bpu10I CCTNAGC 2 cut(s) 291, 780
Bpu1102I GCTNAGC 1 cut(s) 765
BpuMI CCSGG 1 cut(s) 899
BsaJI CCNNGG 3 cut(s) 720, 898, 996
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bsc4I CCNNNNNNNGG 6 cut(s) 31, 688, 897, 1102, 1249, 1338
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 1 cut(s) 1018
BseBI CCWGG 3 cut(s) 892, 998, 1167
BseDI CCNNGG 3 cut(s) 720, 898, 996
BseGI GGATG 5 cut(s) 531, 887, 991, 1143, 1189
BseLI CCNNNNNNNGG 6 cut(s) 31, 688, 897, 1102, 1249, 1338
BseMII CTCAG 3 cut(s) 305, 771, 1041
BseNI ACTGG 1 cut(s) 1018
BseRI GAGGAG 6 cut(s) 148, 186, 189, 564, 768, 1089
BseSI GKGCMC 1 cut(s) 695
BseYI CCCAGC 1 cut(s) 688
BshFI GGCC 3 cut(s) 577, 1031, 1253
BshNI GGYRCC 2 cut(s) 692, 894
BsiSI CCGG 2 cut(s) 77, 898
BslFI GGGAC 1 cut(s) 268
BslI CCNNNNNNNGG 6 cut(s) 31, 688, 897, 1102, 1249, 1338
BsmFI GGGAC 1 cut(s) 268
BsmI GAATGC 2 cut(s) 766, 1147
BsnI GGCC 3 cut(s) 577, 1031, 1253
Bsp1286I GDGCHC 2 cut(s) 131, 695
Bsp143I GATC 3 cut(s) 120, 1080, 1345
Bsp1720I GCTNAGC 1 cut(s) 765
Bsp19I CCATGG 1 cut(s) 720
BspACI CCGC 6 cut(s) 359, 457, 581, 705, 908, 1280
BspANI GGCC 3 cut(s) 577, 1031, 1253
BspCNI CTCAG 3 cut(s) 304, 772, 1042
BspHI TCATGA 1 cut(s) 1231
BspLI GGNNCC 6 cut(s) 283, 284, 694, 896, 942, 1164
BspMAI CTGCAG 2 cut(s) 345, 823
BspPI GGATC 1 cut(s) 1088
BspT107I GGYRCC 2 cut(s) 692, 894
BspTI CTTAAG 1 cut(s) 113
BsrFI RCCGGY 1 cut(s) 76
BsrI ACTGG 1 cut(s) 1018
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 3 cut(s) 720, 898, 996
BssMI GATC 3 cut(s) 120, 1080, 1345
BssT1I CCWWGG 1 cut(s) 720
Bst2UI CCWGG 3 cut(s) 892, 998, 1167
Bst4CI ACNGT 1 cut(s) 734
BstAFI CTTAAG 1 cut(s) 113
BstC8I GCNNGC 3 cut(s) 579, 1039, 1043
BstDEI CTNAG 4 cut(s) 291, 765, 780, 1050
BstDSI CCRYGG 1 cut(s) 720
BstENI CCTNNNNNAGG 1 cut(s) 29
BstF5I GGATG 5 cut(s) 531, 887, 991, 1143, 1189
BstH2I RGCGCY 1 cut(s) 983
BstHHI GCGC 1 cut(s) 982
BstKTI GATC 3 cut(s) 123, 1083, 1348
BstMBI GATC 3 cut(s) 120, 1080, 1345
BstMWI GCNNNNNNNGC 9 cut(s) 295, 388, 391, 463, 466, 472, 713, 722, 892
BstNI CCWGG 3 cut(s) 892, 998, 1167
BstSCI CCNGG 4 cut(s) 890, 897, 996, 1165
BstSFI CTRYAG 2 cut(s) 341, 819
BstSLI GKGCMC 1 cut(s) 695
BstV2I GAAGAC 3 cut(s) 653, 1008, 1296
BstXI CCANNNNNNTGG 1 cut(s) 1158
BsuRI GGCC 3 cut(s) 577, 1031, 1253
BtgI CCRYGG 1 cut(s) 720
BtgZI GCGATG 2 cut(s) 414, 599
BtsCI GGATG 5 cut(s) 531, 887, 991, 1143, 1189
BtsIMutI CAGTG 1 cut(s) 1011
Cac8I GCNNGC 3 cut(s) 579, 1039, 1043
CciI TCATGA 1 cut(s) 1231
CfoI GCGC 1 cut(s) 982
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 2 cut(s) 282, 1163
Csp6I GTAC 1 cut(s) 597
CviAII CATG 6 cut(s) 721, 1064, 1201, 1232, 1271, 1324
CviQI GTAC 1 cut(s) 597
DdeI CTNAG 4 cut(s) 291, 765, 780, 1050
DpnI GATC 3 cut(s) 122, 1082, 1347
DpnII GATC 3 cut(s) 120, 1080, 1345
DriI GACNNNNNGTC 1 cut(s) 336
EaeI YGGCCR 2 cut(s) 1029, 1251
Eam1105I GACNNNNNGTC 1 cut(s) 336
Eco130I CCWWGG 1 cut(s) 720
Eco24I GRGCYC 1 cut(s) 131
Eco32I GATATC 2 cut(s) 420, 450
Eco47I GGWCC 2 cut(s) 282, 1163
Eco57I CTGAAG 2 cut(s) 467, 729
EcoNI CCTNNNNNAGG 1 cut(s) 29
EcoO109I RGGNCCY 2 cut(s) 282, 1163
EcoRII CCWGG 3 cut(s) 890, 996, 1165
EcoRV GATATC 2 cut(s) 420, 450
EcoT14I CCWWGG 1 cut(s) 720
EcoT38I GRGCYC 1 cut(s) 131
ErhI CCWWGG 1 cut(s) 720
FaeI CATG 6 cut(s) 724, 1067, 1204, 1235, 1274, 1327
FaqI GGGAC 1 cut(s) 268
FatI CATG 6 cut(s) 720, 1063, 1200, 1231, 1270, 1323
FauI CCCGC 1 cut(s) 366
FokI GGATG 5 cut(s) 538, 874, 978, 1150, 1196
FriOI GRGCYC 1 cut(s) 131
FspBI CTAG 1 cut(s) 600
GlaI GCGC 1 cut(s) 981
GsaI CCCAGC 1 cut(s) 692
HaeII RGCGCY 1 cut(s) 983
HaeIII GGCC 3 cut(s) 577, 1031, 1253
HapII CCGG 2 cut(s) 77, 898
HhaI GCGC 1 cut(s) 982
Hin1II CATG 6 cut(s) 724, 1067, 1204, 1235, 1274, 1327
Hin6I GCGC 1 cut(s) 980
HinP1I GCGC 1 cut(s) 980
HindIII AAGCTT 3 cut(s) 90, 605, 767
HinfI GANTC 4 cut(s) 511, 676, 868, 1264
HpaII CCGG 2 cut(s) 77, 898
HphI GGTGA 2 cut(s) 1028, 1232
Hpy188I TCNGA 5 cut(s) 214, 952, 1051, 1090, 1263
Hpy188III TCNNGA 5 cut(s) 124, 252, 328, 1232, 1349
HpyAV CCTTC 4 cut(s) 516, 706, 1064, 1342
HpyCH4III ACNGT 1 cut(s) 734
HpyCH4IV ACGT 1 cut(s) 43
HpyCH4V TGCA 6 cut(s) 343, 821, 968, 1145, 1225, 1327
HpyF10VI GCNNNNNNNGC 9 cut(s) 295, 388, 391, 463, 466, 472, 713, 722, 892
HpyF3I CTNAG 4 cut(s) 291, 765, 780, 1050
HpySE526I ACGT 1 cut(s) 43
Hsp92II CATG 6 cut(s) 724, 1067, 1204, 1235, 1274, 1327
HspAI GCGC 1 cut(s) 980
KflI GGGWCCC 1 cut(s) 282
Kzo9I GATC 3 cut(s) 120, 1080, 1345
LmnI GCTCC 2 cut(s) 551, 841
LweI GCATC 4 cut(s) 487, 576, 703, 1081
MaeI CTAG 1 cut(s) 600
MaeII ACGT 1 cut(s) 43
MaeIII GTNAC 2 cut(s) 20, 99
MalI GATC 3 cut(s) 122, 1082, 1347
MboI GATC 3 cut(s) 120, 1080, 1345
MboII GAAGA 8 cut(s) 130, 183, 240, 653, 722, 1013, 1301, 1305
MfeI CAATTG 1 cut(s) 963
MhlI GDGCHC 2 cut(s) 131, 695
MlsI TGGCCA 1 cut(s) 1031
MluNI TGGCCA 1 cut(s) 1031
MlyI GAGTC 3 cut(s) 520, 670, 862
MmeI TCCRAC 2 cut(s) 1176, 1233
Mox20I TGGCCA 1 cut(s) 1031
MscI TGGCCA 1 cut(s) 1031
MseI TTAA 2 cut(s) 114, 771
Msp20I TGGCCA 1 cut(s) 1031
MspA1I CMGCKG 3 cut(s) 581, 707, 824
MspCI CTTAAG 1 cut(s) 113
MspI CCGG 2 cut(s) 77, 898
MspR9I CCNGG 4 cut(s) 892, 899, 998, 1167
MunI CAATTG 1 cut(s) 963
Mva1269I GAATGC 2 cut(s) 766, 1147
MvaI CCWGG 3 cut(s) 892, 998, 1167
MwoI GCNNNNNNNGC 9 cut(s) 295, 388, 391, 463, 466, 472, 713, 722, 892
NciI CCSGG 1 cut(s) 899
NcoI CCATGG 1 cut(s) 720
NdeII GATC 3 cut(s) 120, 1080, 1345
NlaIII CATG 6 cut(s) 724, 1067, 1204, 1235, 1274, 1327
NlaIV GGNNCC 6 cut(s) 283, 284, 694, 896, 942, 1164
NmeAIII GCCGAG 1 cut(s) 38
NmuCI GTSAC 2 cut(s) 20, 99
PagI TCATGA 1 cut(s) 1231
PcsI WCGNNNNNNNCGW 1 cut(s) 664
PctI GAATGC 2 cut(s) 766, 1147
PfeI GAWTC 1 cut(s) 1264
PleI GAGTC 3 cut(s) 519, 670, 862
PpsI GAGTC 3 cut(s) 519, 670, 862
PpuMI RGGWCCY 2 cut(s) 282, 1163
Psp5II RGGWCCY 2 cut(s) 282, 1163
Psp6I CCWGG 3 cut(s) 890, 996, 1165
PspFI CCCAGC 1 cut(s) 688
PspGI CCWGG 3 cut(s) 890, 996, 1165
PspN4I GGNNCC 6 cut(s) 283, 284, 694, 896, 942, 1164
PspPI GGNCC 2 cut(s) 282, 1163
PspPPI RGGWCCY 2 cut(s) 282, 1163
PstI CTGCAG 2 cut(s) 345, 823
PvuII CAGCTG 1 cut(s) 824
RsaI GTAC 1 cut(s) 598
RsaNI GTAC 1 cut(s) 597
SaqAI TTAA 2 cut(s) 114, 771
Sau3AI GATC 3 cut(s) 120, 1080, 1345
Sau96I GGNCC 2 cut(s) 282, 1163
ScaI AGTACT 1 cut(s) 598
SchI GAGTC 3 cut(s) 520, 670, 862
ScrFI CCNGG 4 cut(s) 892, 899, 998, 1167
SduI GDGCHC 2 cut(s) 131, 695
SfaNI GCATC 4 cut(s) 487, 576, 703, 1081
SfcI CTRYAG 2 cut(s) 341, 819
SinI GGWCC 2 cut(s) 282, 1163
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
SpeI ACTAGT 1 cut(s) 599
SsiI CCGC 6 cut(s) 359, 457, 581, 705, 908, 1280
SspMI CTAG 1 cut(s) 600
StyD4I CCNGG 4 cut(s) 890, 897, 996, 1165
StyI CCWWGG 1 cut(s) 720
TaaI ACNGT 1 cut(s) 734
TaiI ACGT 1 cut(s) 46
TaqI TCGA 2 cut(s) 667, 699
TatI WGTACW 1 cut(s) 596
TauI GCSGC 3 cut(s) 460, 584, 910
TfiI GAWTC 1 cut(s) 1264
Tru1I TTAA 2 cut(s) 114, 771
Tru9I TTAA 2 cut(s) 114, 771
TscAI CASTG 1 cut(s) 1018
TseFI GTSAC 2 cut(s) 20, 99
Tsp45I GTSAC 2 cut(s) 20, 99
TspDTI ATGAA 8 cut(s) 102, 170, 198, 551, 1154, 1287, 1299, 1305
TspRI CASTG 1 cut(s) 1018
Vha464I CTTAAG 1 cut(s) 113
VpaK11BI GGWCC 2 cut(s) 282, 1163
XagI CCTNNNNNAGG 1 cut(s) 29
XapI RAATTY 3 cut(s) 176, 505, 654
XspI CTAG 1 cut(s) 600
ZrmI AGTACT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.