Rmu_ssc0000301.1_g000001

Histone-binding protein RBBP4 or subunit C of CAF1 complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000301.1
OG0003346
Physical Location & Seq
Forward (+)
1 .. 3446
3446 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 873 bp
gtgaacagaatcagggaactcccacagaatagtaagattgtggccacacacactgacagtcctgatgttctcatttgggacattgaggctcaacctaatcgtcatgctgtcctgggagctacaaattctcgcccagatctgctattgactggacatcaagataatgctgaatttgcacttgctatgtgcccctcggaaccctttgtactctctggagggaaggacaagtcagtgattttgtggagtattcaagatcatatctcgtctttggcatcagatcctgggtctgcaaggtctccaggatctggtggttcaaatgctaaacatgcctctaaggttggtgggagtaatgacaaacccacagacagcccttctgttgagccacgaggtatatatcaagggcatgaggatactgttgaagatgtacagttctgcccatctagtgcgcaggagttctgcagtgtaggcgacgattcttgcctcatactatgggatgctagatctggcaatagtccggttgtcaaggttgagaaagctcatgatgctgaccttcattgtgtagattggaatcctcatgatgtcaatctcattcttacagggtctgctgataagactgttcgtatgtttgaccgccggaatctcacttctggaggagttggatcacccattcataagtttgagggccacaacgaagctgttctctgtgttcagtggtctcctgacaagtcctctgtctttggaagttctgctgaggatggcctattgaacatctgggatcatgaaaagattggtaagaagcaagaatctgttggatcaaagctgccaaatgctcctcctgcaggattgtttttccgacatgctggtcataggtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04218 map04218
Pfam Domains
Protein Families
CAF1C_H4-bd WD40

Protein Analysis

290

Amino Acids

31.2

Weight (kDa)

5.64

Isoelectric Point (pI)

52.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_TEP1_2nd PF25047 123 - 263 2.5e-10 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 124 - 211 3.9e-09 CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 131 - 212 5.6e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 132 - 171 4e-06 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 132 - 262 8.2e-21 WDR5 beta-propeller domain
WDR55 PF24796 133 - 262 2.1e-08 WDR55
Beta-prop_WDR3_1st PF25173 133 - 210 8e-06 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 133 - 212 4.3e-06 WDR3 second beta-propeller domain
Beta-prop_EIPR1 PF23609 135 - 209 4.7e-10 EIPR1 beta-propeller
Beta-prop_EML PF23409 135 - 262 5e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_Aladin PF25460 138 - 261 7.7e-06 Aladin seven-bladed propeller
Beta-prop_EML_2 PF23414 148 - 267 1.9e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 154 - 260 4.3e-10 WDR3 first beta-propeller domain
Beta-prop_EIPR1 PF23609 178 - 260 1.3e-06 EIPR1 beta-propeller
WD40_Prp19 PF24814 178 - 262 7.2e-11 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 180 - 271 2.9e-13 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 182 - 262 8e-07 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 199 - 262 7e-06 CDC20/Fizzy WD40 domain
WD40 PF00400 220 - 259 8.3e-08 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 861
Acc16I TGCGCA 1 cut(s) 447
AccB7I CCANNNNNTGG 1 cut(s) 305
AciI CCGC 1 cut(s) 631
AclWI GGATC 5 cut(s) 272, 310, 667, 783, 820
AcoI YGGCCR 1 cut(s) 42
AcsI RAATTY 2 cut(s) 124, 170
AfaI GTAC 2 cut(s) 207, 426
AfiI CCNNNNNNNGG 2 cut(s) 305, 839
AgsI TTSAA 4 cut(s) 251, 315, 419, 766
AjnI CCWGG 3 cut(s) 111, 280, 298
AluBI AGCT 4 cut(s) 119, 536, 695, 820
AluI AGCT 4 cut(s) 119, 536, 695, 820
Alw26I GTCTC 2 cut(s) 300, 720
AlwI GGATC 5 cut(s) 272, 310, 667, 783, 820
AlwNI CAGNNNCTG 3 cut(s) 281, 305, 602
AoxI GGCC 3 cut(s) 42, 682, 757
ApeKI GCWGC 1 cut(s) 820
ApoI RAATTY 2 cut(s) 124, 170
Asp700I GAANNNNTTC 1 cut(s) 696
AspLEI GCGC 1 cut(s) 448
AspS9I GGNCC 1 cut(s) 682
AsuHPI GGTGA 1 cut(s) 654
BaeGI GKGCMC 1 cut(s) 191
BalI TGGCCA 1 cut(s) 44
BauI CACGAG 1 cut(s) 384
BbvCI CCTCAGC 1 cut(s) 750
BbvI GCAGC 1 cut(s) 807
BccI CCATC 2 cut(s) 445, 749
BciT130I CCWGG 3 cut(s) 113, 282, 300
BciVI GTATCC 1 cut(s) 403
BcoDI GTCTC 2 cut(s) 300, 720
BfaI CTAG 2 cut(s) 441, 498
BfmI CTRYAG 2 cut(s) 457, 837
BfuI GTATCC 1 cut(s) 403
BglII AGATCT 2 cut(s) 136, 500
BisI GCNGC 1 cut(s) 821
BlsI GCNGC 1 cut(s) 822
Bme1390I CCNGG 3 cut(s) 113, 282, 300
BmgT120I GGNCC 1 cut(s) 682
BmiI GGNNCC 1 cut(s) 198
BmrFI CCNGG 3 cut(s) 113, 282, 300
BmsI GCATC 3 cut(s) 281, 484, 532
BpmI CTGGAG 3 cut(s) 234, 282, 669
Bpu10I CCTNAGC 1 cut(s) 750
BsaBI GATNNNNATC 2 cut(s) 567, 582
BsaI GGTCTC 2 cut(s) 300, 720
BsaJI CCNNGG 3 cut(s) 112, 192, 281
BsaWI WCCGGW 1 cut(s) 514
Bsc4I CCNNNNNNNGG 2 cut(s) 305, 839
Bse1I ACTGG 1 cut(s) 154
Bse8I GATNNNNATC 2 cut(s) 567, 582
BseBI CCWGG 3 cut(s) 113, 282, 300
BseDI CCNNGG 3 cut(s) 112, 192, 281
BseGI GGATG 2 cut(s) 499, 760
BseJI GATNNNNATC 2 cut(s) 567, 582
BseLI CCNNNNNNNGG 2 cut(s) 305, 839
BseMII CTCAG 1 cut(s) 741
BseNI ACTGG 1 cut(s) 154
BseRI GAGGAG 2 cut(s) 666, 822
BseSI GKGCMC 1 cut(s) 191
BseXI GCAGC 1 cut(s) 807
BshFI GGCC 3 cut(s) 44, 684, 759
BsiSI CCGG 2 cut(s) 515, 634
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 2 cut(s) 305, 839
BsmAI GTCTC 2 cut(s) 300, 720
BsmFI GGGAC 1 cut(s) 92
BsnI GGCC 3 cut(s) 44, 684, 759
Bso31I GGTCTC 2 cut(s) 300, 720
Bsp1286I GDGCHC 1 cut(s) 191
Bsp1407I TGTACA 1 cut(s) 424
Bsp143I GATC 8 cut(s) 136, 253, 277, 302, 500, 659, 775, 812
BspACI CCGC 1 cut(s) 631
BspANI GGCC 3 cut(s) 44, 684, 759
BspCNI CTCAG 1 cut(s) 742
BspHI TCATGA 3 cut(s) 538, 574, 778
BspLI GGNNCC 1 cut(s) 198
BspMAI CTGCAG 2 cut(s) 461, 841
BspPI GGATC 5 cut(s) 272, 310, 667, 783, 820
BspTNI GGTCTC 2 cut(s) 300, 720
BsrGI TGTACA 1 cut(s) 424
BsrI ACTGG 1 cut(s) 154
BssECI CCNNGG 3 cut(s) 112, 192, 281
BssMI GATC 8 cut(s) 136, 253, 277, 302, 500, 659, 775, 812
BssSI CACGAG 1 cut(s) 384
Bst2BI CACGAG 1 cut(s) 384
Bst2UI CCWGG 3 cut(s) 113, 282, 300
Bst4CI ACNGT 4 cut(s) 59, 415, 429, 616
BstAUI TGTACA 1 cut(s) 424
BstDEI CTNAG 2 cut(s) 333, 750
BstENI CCTNNNNNAGG 1 cut(s) 837
BstF5I GGATG 2 cut(s) 499, 760
BstHHI GCGC 1 cut(s) 448
BstKTI GATC 8 cut(s) 139, 256, 280, 305, 503, 662, 778, 815
BstMAI GTCTC 2 cut(s) 300, 720
BstMBI GATC 8 cut(s) 136, 253, 277, 302, 500, 659, 775, 812
BstMWI GCNNNNNNNGC 5 cut(s) 173, 326, 465, 542, 836
BstNI CCWGG 3 cut(s) 113, 282, 300
BstNSI RCATGY 2 cut(s) 329, 860
BstSCI CCNGG 3 cut(s) 111, 280, 298
BstSFI CTRYAG 2 cut(s) 457, 837
BstSLI GKGCMC 1 cut(s) 191
BstV1I GCAGC 1 cut(s) 807
BstX2I RGATCY 4 cut(s) 136, 277, 302, 500
BstYI RGATCY 4 cut(s) 136, 277, 302, 500
BsuI GTATCC 1 cut(s) 403
BsuRI GGCC 3 cut(s) 44, 684, 759
BtsCI GGATG 2 cut(s) 499, 760
BtsI GCAGTG 1 cut(s) 466
BtsIMutI CAGTG 4 cut(s) 51, 237, 466, 716
CaiI CAGNNNCTG 3 cut(s) 281, 305, 602
CciI TCATGA 3 cut(s) 538, 574, 778
CfoI GCGC 1 cut(s) 448
Cfr13I GGNCC 1 cut(s) 682
Csp6I GTAC 2 cut(s) 206, 425
CviAII CATG 7 cut(s) 104, 326, 404, 539, 575, 779, 857
CviQI GTAC 2 cut(s) 206, 425
DdeI CTNAG 2 cut(s) 333, 750
DpnI GATC 8 cut(s) 138, 255, 279, 304, 502, 661, 777, 814
DpnII GATC 8 cut(s) 136, 253, 277, 302, 500, 659, 775, 812
DrdI GACNNNNNNGTC 1 cut(s) 861
DseDI GACNNNNNNGTC 1 cut(s) 861
EaeI YGGCCR 1 cut(s) 42
Eco31I GGTCTC 2 cut(s) 300, 720
EcoNI CCTNNNNNAGG 1 cut(s) 837
EcoRII CCWGG 3 cut(s) 111, 280, 298
FaeI CATG 7 cut(s) 107, 329, 407, 542, 578, 782, 860
FaqI GGGAC 1 cut(s) 92
FatI CATG 7 cut(s) 103, 325, 403, 538, 574, 778, 856
Fnu4HI GCNGC 1 cut(s) 821
FokI GGATG 2 cut(s) 506, 767
Fsp4HI GCNGC 1 cut(s) 821
FspBI CTAG 2 cut(s) 441, 498
FspI TGCGCA 1 cut(s) 447
GlaI GCGC 1 cut(s) 447
GluI GCNGC 1 cut(s) 821
GsuI CTGGAG 3 cut(s) 234, 282, 669
HaeIII GGCC 3 cut(s) 44, 684, 759
HapII CCGG 2 cut(s) 515, 634
HhaI GCGC 1 cut(s) 448
Hin1II CATG 7 cut(s) 107, 329, 407, 542, 578, 782, 860
Hin6I GCGC 1 cut(s) 446
HinP1I GCGC 1 cut(s) 446
HinfI GANTC 5 cut(s) 9, 473, 568, 637, 803
HpaII CCGG 2 cut(s) 515, 634
HphI GGTGA 1 cut(s) 654
Hpy166II GTNNAC 1 cut(s) 4
Hpy188I TCNGA 3 cut(s) 196, 277, 854
Hpy188III TCNNGA 9 cut(s) 62, 158, 213, 251, 539, 575, 648, 719, 779
Hpy8I GTNNAC 1 cut(s) 4
Hpy99I CGWCG 1 cut(s) 473
HpyAV CCTTC 3 cut(s) 214, 381, 560
HpyCH4III ACNGT 4 cut(s) 59, 415, 429, 616
HpyCH4V TGCA 4 cut(s) 176, 290, 459, 839
HpyF10VI GCNNNNNNNGC 5 cut(s) 173, 326, 465, 542, 836
HpyF3I CTNAG 2 cut(s) 333, 750
Hsp92II CATG 7 cut(s) 107, 329, 407, 542, 578, 782, 860
HspAI GCGC 1 cut(s) 446
Kzo9I GATC 8 cut(s) 136, 253, 277, 302, 500, 659, 775, 812
LmnI GCTCC 2 cut(s) 116, 835
Lsp1109I GCAGC 1 cut(s) 807
LweI GCATC 3 cut(s) 281, 484, 532
MaeI CTAG 2 cut(s) 441, 498
MalI GATC 8 cut(s) 138, 255, 279, 304, 502, 661, 777, 814
MboI GATC 8 cut(s) 136, 253, 277, 302, 500, 659, 775, 812
MboII GAAGA 1 cut(s) 431
MflI RGATCY 4 cut(s) 136, 277, 302, 500
MhlI GDGCHC 1 cut(s) 191
MlsI TGGCCA 1 cut(s) 44
MluCI AATT 2 cut(s) 124, 170
MluNI TGGCCA 1 cut(s) 44
MmeI TCCRAC 2 cut(s) 637, 790
Mox20I TGGCCA 1 cut(s) 44
MroXI GAANNNNTTC 1 cut(s) 696
MscI TGGCCA 1 cut(s) 44
Msp20I TGGCCA 1 cut(s) 44
MspI CCGG 2 cut(s) 515, 634
MspR9I CCNGG 3 cut(s) 113, 282, 300
MvaI CCWGG 3 cut(s) 113, 282, 300
MwoI GCNNNNNNNGC 5 cut(s) 173, 326, 465, 542, 836
NdeII GATC 8 cut(s) 136, 253, 277, 302, 500, 659, 775, 812
NlaIII CATG 7 cut(s) 107, 329, 407, 542, 578, 782, 860
NlaIV GGNNCC 1 cut(s) 198
NsbI TGCGCA 1 cut(s) 447
NspI RCATGY 2 cut(s) 329, 860
PagI TCATGA 3 cut(s) 538, 574, 778
PdmI GAANNNNTTC 1 cut(s) 696
PfeI GAWTC 5 cut(s) 9, 473, 568, 637, 803
PflMI CCANNNNNTGG 1 cut(s) 305
PfoI TCCNGGA 1 cut(s) 298
PkrI GCNGC 1 cut(s) 822
Psp6I CCWGG 3 cut(s) 111, 280, 298
PspGI CCWGG 3 cut(s) 111, 280, 298
PspN4I GGNNCC 1 cut(s) 198
PspPI GGNCC 1 cut(s) 682
PsrI GAACNNNNNNTAC 2 cut(s) 189, 221
PstI CTGCAG 2 cut(s) 461, 841
PstNI CAGNNNCTG 3 cut(s) 281, 305, 602
PsuI RGATCY 4 cut(s) 136, 277, 302, 500
RsaI GTAC 2 cut(s) 207, 426
RsaNI GTAC 2 cut(s) 206, 425
SatI GCNGC 1 cut(s) 821
Sau3AI GATC 8 cut(s) 136, 253, 277, 302, 500, 659, 775, 812
Sau96I GGNCC 1 cut(s) 682
SbfI CCTGCAGG 1 cut(s) 841
ScrFI CCNGG 3 cut(s) 113, 282, 300
SdaI CCTGCAGG 1 cut(s) 841
SduI GDGCHC 1 cut(s) 191
SfaNI GCATC 3 cut(s) 281, 484, 532
SfcI CTRYAG 2 cut(s) 457, 837
Sse8387I CCTGCAGG 1 cut(s) 841
Sse9I AATT 2 cut(s) 124, 170
SsiI CCGC 1 cut(s) 631
SspMI CTAG 2 cut(s) 441, 498
StyD4I CCNGG 3 cut(s) 111, 280, 298
TaaI ACNGT 4 cut(s) 59, 415, 429, 616
TasI AATT 2 cut(s) 124, 170
TatI WGTACW 2 cut(s) 205, 424
TfiI GAWTC 5 cut(s) 9, 473, 568, 637, 803
TscAI CASTG 4 cut(s) 58, 237, 466, 716
TseI GCWGC 1 cut(s) 820
TspDTI ATGAA 3 cut(s) 542, 659, 795
TspRI CASTG 4 cut(s) 58, 237, 466, 716
Van91I CCANNNNNTGG 1 cut(s) 305
XagI CCTNNNNNAGG 1 cut(s) 837
XapI RAATTY 2 cut(s) 124, 170
XceI RCATGY 2 cut(s) 329, 860
XmnI GAANNNNTTC 1 cut(s) 696
XspI CTAG 2 cut(s) 441, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.