Rmu_ssc0000316.1_g000004

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000316.1
OG0005601
Physical Location & Seq
Forward (+)
11491 .. 12566
1076 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000316.1_g000004.1.cds

Sequence Viewer

Length: 459 bp
atgatctggcttcaccgtcgagtacttcctcaggtacttcttgattccggcgacgaggcattaattgtcgcgcatgccttcgtcgatggccttcacaatgatgcctttgaggccggtgaagcgaccctagaagaggatcacaaatttgacaacttcgccgatcccatcttccccctccgatcgccgcccatggagagccagcagagctcttgcctgagctggctggggatgcacgtggcctcggccttcttcgcttctctggaacgttgctcgtgcatcaacctatctactcacgacgacaacgacttcaccaccaaccccgaggaggcacacgaccgacctctcatgctcttctctcgctccgtcaatctcagcgatcaccgcccccagtcgttcccctccacgtcgacgacgcccggccatcctaacctaaacgacgtcgccaagctcccagtctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

152

Amino Acids

16.94

Weight (kDa)

4.82

Isoelectric Point (pI)

63.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 441
AccI GTMKAC 1 cut(s) 407
AccII CGCG 1 cut(s) 71
AciI CCGC 2 cut(s) 185, 382
AclI AACGTT 1 cut(s) 265
AclWI GGATC 2 cut(s) 144, 155
AcoI YGGCCR 1 cut(s) 418
AcsI RAATTY 1 cut(s) 143
AcvI CACGTG 1 cut(s) 235
AcyI GRCGYC 2 cut(s) 413, 438
AfaI GTAC 2 cut(s) 24, 36
AfiI CCNNNNNNNGG 2 cut(s) 133, 325
AjiI CACGTC 1 cut(s) 405
AluBI AGCT 3 cut(s) 207, 219, 448
AluI AGCT 3 cut(s) 207, 219, 448
Alw21I GWGCWC 1 cut(s) 209
AlwI GGATC 2 cut(s) 144, 155
Ama87I CYCGRG 1 cut(s) 320
AoxI GGCC 5 cut(s) 88, 111, 237, 243, 418
ApoI RAATTY 1 cut(s) 143
AseI ATTAAT 1 cut(s) 62
AspLEI GCGC 1 cut(s) 73
AsuC2I CCSGG 1 cut(s) 417
AsuHPI GGTGA 4 cut(s) 5, 128, 301, 371
AvaI CYCGRG 1 cut(s) 320
AxyI CCTNAGG 1 cut(s) 30
BanII GRGCYC 1 cut(s) 209
BauI CACGAG 1 cut(s) 271
BbrPI CACGTG 1 cut(s) 235
Bbv12I GWGCWC 1 cut(s) 209
BccI CCATC 3 cut(s) 80, 173, 429
BcnI CCSGG 1 cut(s) 417
BfaI CTAG 1 cut(s) 128
BglI GCCNNNNNGGC 1 cut(s) 110
BisI GCNGC 1 cut(s) 185
BlsI GCNGC 1 cut(s) 186
BmcAI AGTACT 1 cut(s) 24
Bme1390I CCNGG 1 cut(s) 417
BmeT110I CYCGRG 1 cut(s) 320
BmgBI CACGTC 1 cut(s) 405
BmrFI CCNGG 1 cut(s) 417
BmrI ACTGGG 2 cut(s) 382, 446
BmsI GCATC 3 cut(s) 91, 219, 285
BmuI ACTGGG 2 cut(s) 382, 446
Bpu10I CCTNAGC 1 cut(s) 215
BpuMI CCSGG 1 cut(s) 417
BsaAI YACGTR 1 cut(s) 235
BsaHI GRCGYC 2 cut(s) 413, 438
BsaJI CCNNGG 3 cut(s) 189, 240, 321
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 325
Bse118I RCCGGY 1 cut(s) 113
Bse1I ACTGG 2 cut(s) 388, 452
Bse21I CCTNAGG 1 cut(s) 30
BseDI CCNNGG 3 cut(s) 189, 240, 321
BseGI GGATG 2 cut(s) 234, 421
BseLI CCNNNNNNNGG 2 cut(s) 133, 325
BseMII CTCAG 3 cut(s) 44, 206, 385
BseNI ACTGG 2 cut(s) 388, 452
BseRI GAGGAG 1 cut(s) 338
BseYI CCCAGC 1 cut(s) 223
Bsh1236I CGCG 1 cut(s) 71
Bsh1285I CGRYCG 2 cut(s) 182, 337
BshFI GGCC 5 cut(s) 90, 113, 239, 245, 420
BsiEI CGRYCG 2 cut(s) 182, 337
BsiHKAI GWGCWC 1 cut(s) 209
BsiHKCI CYCGRG 1 cut(s) 320
BsiSI CCGG 3 cut(s) 48, 114, 417
BslI CCNNNNNNNGG 2 cut(s) 133, 325
BsnI GGCC 5 cut(s) 90, 113, 239, 245, 420
BsoBI CYCGRG 1 cut(s) 320
Bsp1286I GDGCHC 1 cut(s) 209
Bsp143I GATC 5 cut(s) 3, 136, 160, 179, 376
Bsp19I CCATGG 1 cut(s) 189
BspACI CCGC 2 cut(s) 185, 382
BspANI GGCC 5 cut(s) 90, 113, 239, 245, 420
BspCNI CTCAG 3 cut(s) 43, 207, 384
BspFNI CGCG 1 cut(s) 71
BspPI GGATC 2 cut(s) 144, 155
BspQI GCTCTTC 1 cut(s) 356
BsrFI RCCGGY 1 cut(s) 113
BsrI ACTGG 2 cut(s) 388, 452
BssAI RCCGGY 1 cut(s) 113
BssECI CCNNGG 3 cut(s) 189, 240, 321
BssMI GATC 5 cut(s) 3, 136, 160, 179, 376
BssNI GRCGYC 2 cut(s) 413, 438
BssSI CACGAG 1 cut(s) 271
BssT1I CCWWGG 1 cut(s) 189
Bst2BI CACGAG 1 cut(s) 271
Bst4CI ACNGT 1 cut(s) 17
Bst6I CTCTTC 2 cut(s) 126, 356
BstACI GRCGYC 2 cut(s) 413, 438
BstBAI YACGTR 1 cut(s) 235
BstC8I GCNNGC 3 cut(s) 75, 200, 221
BstDEI CTNAG 3 cut(s) 30, 215, 371
BstDSI CCRYGG 1 cut(s) 189
BstENI CCTNNNNNAGG 1 cut(s) 131
BstF5I GGATG 2 cut(s) 234, 421
BstFNI CGCG 1 cut(s) 71
BstHHI GCGC 1 cut(s) 73
BstKTI GATC 5 cut(s) 6, 139, 163, 182, 379
BstMBI GATC 5 cut(s) 3, 136, 160, 179, 376
BstMCI CGRYCG 2 cut(s) 182, 337
BstMWI GCNNNNNNNGC 6 cut(s) 110, 119, 204, 229, 251, 381
BstNSI RCATGY 1 cut(s) 77
BstSCI CCNGG 1 cut(s) 415
BstUI CGCG 1 cut(s) 71
Bsu36I CCTNAGG 1 cut(s) 30
BsuRI GGCC 5 cut(s) 90, 113, 239, 245, 420
BtgI CCRYGG 1 cut(s) 189
BtrI CACGTC 1 cut(s) 405
BtsCI GGATG 2 cut(s) 234, 421
Cac8I GCNNGC 3 cut(s) 75, 200, 221
CfoI GCGC 1 cut(s) 73
Cfr10I RCCGGY 1 cut(s) 113
CseI GACGC 1 cut(s) 421
Csp6I GTAC 2 cut(s) 23, 35
CviAII CATG 3 cut(s) 74, 190, 346
CviQI GTAC 2 cut(s) 23, 35
DdeI CTNAG 3 cut(s) 30, 215, 371
DpnI GATC 5 cut(s) 5, 138, 162, 181, 378
DpnII GATC 5 cut(s) 3, 136, 160, 179, 376
EaeI YGGCCR 1 cut(s) 418
Eam1104I CTCTTC 2 cut(s) 126, 356
EarI CTCTTC 2 cut(s) 126, 356
Ecl136II GAGCTC 1 cut(s) 207
Eco130I CCWWGG 1 cut(s) 189
Eco24I GRGCYC 1 cut(s) 209
Eco53kI GAGCTC 1 cut(s) 207
Eco72I CACGTG 1 cut(s) 235
Eco81I CCTNAGG 1 cut(s) 30
Eco88I CYCGRG 1 cut(s) 320
EcoICRI GAGCTC 1 cut(s) 207
EcoNI CCTNNNNNAGG 1 cut(s) 131
EcoT14I CCWWGG 1 cut(s) 189
EcoT38I GRGCYC 1 cut(s) 209
ErhI CCWWGG 1 cut(s) 189
FaeI CATG 3 cut(s) 77, 193, 349
FaiI YATR 3 cut(s) 75, 191, 347
FatI CATG 3 cut(s) 73, 189, 345
FblI GTMKAC 1 cut(s) 407
Fnu4HI GCNGC 1 cut(s) 185
FokI GGATG 2 cut(s) 241, 408
FriOI GRGCYC 1 cut(s) 209
Fsp4HI GCNGC 1 cut(s) 185
FspBI CTAG 1 cut(s) 128
GlaI GCGC 1 cut(s) 72
GluI GCNGC 1 cut(s) 185
GsaI CCCAGC 1 cut(s) 227
HaeIII GGCC 5 cut(s) 90, 113, 239, 245, 420
HapII CCGG 3 cut(s) 48, 114, 417
HgaI GACGC 1 cut(s) 421
HhaI GCGC 1 cut(s) 73
Hin1I GRCGYC 2 cut(s) 413, 438
Hin1II CATG 3 cut(s) 77, 193, 349
Hin6I GCGC 1 cut(s) 71
HinP1I GCGC 1 cut(s) 71
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HinfI GANTC 1 cut(s) 44
HpaII CCGG 3 cut(s) 48, 114, 417
HphI GGTGA 4 cut(s) 5, 128, 301, 371
Hpy166II GTNNAC 1 cut(s) 408
Hpy188I TCNGA 2 cut(s) 179, 458
Hpy188III TCNNGA 3 cut(s) 41, 260, 293
Hpy8I GTNNAC 1 cut(s) 408
Hpy99I CGWCG 9 cut(s) 21, 56, 86, 299, 409, 412, 415, 440, 443
HpyAV CCTTC 3 cut(s) 88, 101, 256
HpyCH4III ACNGT 1 cut(s) 17
HpyCH4IV ACGT 4 cut(s) 234, 265, 404, 438
HpyCH4V TGCA 2 cut(s) 232, 276
HpyF10VI GCNNNNNNNGC 6 cut(s) 110, 119, 204, 229, 251, 381
HpyF3I CTNAG 3 cut(s) 30, 215, 371
HpySE526I ACGT 4 cut(s) 234, 265, 404, 438
Hsp92I GRCGYC 2 cut(s) 413, 438
Hsp92II CATG 3 cut(s) 77, 193, 349
HspAI GCGC 1 cut(s) 71
Kzo9I GATC 5 cut(s) 3, 136, 160, 179, 376
LguI GCTCTTC 1 cut(s) 356
LmnI GCTCC 2 cut(s) 365, 453
LweI GCATC 3 cut(s) 91, 219, 285
MaeI CTAG 1 cut(s) 128
MaeII ACGT 4 cut(s) 234, 265, 404, 438
MalI GATC 5 cut(s) 5, 138, 162, 181, 378
MboI GATC 5 cut(s) 3, 136, 160, 179, 376
MboII GAAGA 4 cut(s) 143, 160, 241, 343
MhlI GDGCHC 1 cut(s) 209
MluCI AATT 2 cut(s) 63, 143
MseI TTAA 1 cut(s) 62
MslI CAYNNNNRTG 1 cut(s) 99
MspI CCGG 3 cut(s) 48, 114, 417
MspR9I CCNGG 1 cut(s) 417
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 6 cut(s) 110, 119, 204, 229, 251, 381
NciI CCSGG 1 cut(s) 417
NcoI CCATGG 1 cut(s) 189
NdeII GATC 5 cut(s) 3, 136, 160, 179, 376
NlaIII CATG 3 cut(s) 77, 193, 349
NmeAIII GCCGAG 1 cut(s) 221
NspI RCATGY 1 cut(s) 77
PaeI GCATGC 1 cut(s) 77
PciSI GCTCTTC 1 cut(s) 356
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PfeI GAWTC 1 cut(s) 44
PkrI GCNGC 1 cut(s) 186
Ple19I CGATCG 1 cut(s) 182
PmaCI CACGTG 1 cut(s) 235
PmlI CACGTG 1 cut(s) 235
Ppu21I YACGTR 1 cut(s) 235
PshBI ATTAAT 1 cut(s) 62
Psp124BI GAGCTC 1 cut(s) 209
Psp1406I AACGTT 1 cut(s) 265
PspCI CACGTG 1 cut(s) 235
PspFI CCCAGC 1 cut(s) 223
PvuI CGATCG 1 cut(s) 182
RsaI GTAC 2 cut(s) 24, 36
RsaNI GTAC 2 cut(s) 23, 35
RseI CAYNNNNRTG 1 cut(s) 99
SacI GAGCTC 1 cut(s) 209
SalI GTCGAC 1 cut(s) 406
SapI GCTCTTC 1 cut(s) 356
SaqAI TTAA 1 cut(s) 62
SatI GCNGC 1 cut(s) 185
Sau3AI GATC 5 cut(s) 3, 136, 160, 179, 376
ScaI AGTACT 1 cut(s) 24
ScrFI CCNGG 1 cut(s) 417
SduI GDGCHC 1 cut(s) 209
SfaNI GCATC 3 cut(s) 91, 219, 285
SgrDI CGTCGACG 1 cut(s) 406
SmiMI CAYNNNNRTG 1 cut(s) 99
SphI GCATGC 1 cut(s) 77
Sse9I AATT 2 cut(s) 63, 143
SsiI CCGC 2 cut(s) 185, 382
SspMI CTAG 1 cut(s) 128
SstI GAGCTC 1 cut(s) 209
StyD4I CCNGG 1 cut(s) 415
StyI CCWWGG 1 cut(s) 189
TaaI ACNGT 1 cut(s) 17
TaiI ACGT 4 cut(s) 237, 268, 407, 441
TaqI TCGA 3 cut(s) 19, 84, 407
TaqII GACCGA 1 cut(s) 351
TasI AATT 2 cut(s) 63, 143
TatI WGTACW 1 cut(s) 22
TauI GCSGC 1 cut(s) 187
TfiI GAWTC 1 cut(s) 44
Tru1I TTAA 1 cut(s) 62
Tru9I TTAA 1 cut(s) 62
TspGWI ACGGA 1 cut(s) 352
VspI ATTAAT 1 cut(s) 62
XagI CCTNNNNNAGG 1 cut(s) 131
XapI RAATTY 1 cut(s) 143
XceI RCATGY 1 cut(s) 77
XmiI GTMKAC 1 cut(s) 407
XspI CTAG 1 cut(s) 128
ZraI GACGTC 1 cut(s) 439
ZrmI AGTACT 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.