Rmu_ssc0000358.1_g000003
ERF Family

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. TRX MLL subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000358.1
OG0003180
Physical Location & Seq
Reverse (-)
7072 .. 18860
11789 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4230 bp
atgataattaagcggccgtcgagatttgaaatgccgaatttgaaacggtgtaagctggaggaggcggaagctaaggcctatgcgtattcggcgaatccgaagaggcggaaagcgaatgggtactgctcagttggcaatggcatgggggaggatttgagcagcggctccggttcgtcgggatacgacgagtcgttttcggctgcggtcgaggtggtggagtcgaatttgcagcggttgagtaagcggcgagaaatgaatcggaggtccgagaagttgaggaggtctgctcgaggacggccgcaagtgccgtcttcgaggtttaacggttcaggtgagaaaaagcaagcgaaatatgtagatacatatacggtttttgaagatgaagaagaatgtgatgttttagcagtggaggacagagaagggattgagaaattgggttttgttaaaaagcgtaaggaggagatcaatgggtttaagaagttgaagaagttccaatattgtgggaaggtagagcaagaagaagtggattattatgatgatgttggttacagacagtatatgggtttgaaaaattcgatagcttcgaaacgtggtggaaattctttgccagaggaaagaagtgaggaatatggagctgggtttagttttggcggcaaggagaaagttgacaaacccaattgcgcaaagaagaaagaagtgtataagccgcaggactttgctttgggtgatattgtttgggctaagtgtgggaggagctacccagtttggccggcggtggttatcgaccccatattgcaagctccagaagcagtgttgagctgctgtattcctaatgctatctgtgtcatgttctttgggttctcaaagaatggaacacaaagggattatgggtgggtgaagcagggaatgatatatccgttcaagcagttccttgagagatttcgaggacagacccagttgtacaggagtaaaccaagcgagcttcagatggcaatagaggaggcggtgttagcagaaaatggctttatttacgcagcttgtaaaactactgattcagaggcaaatgcccctgaaatccaagaggccacagatttttatcaagatcaggcatattacagtcagaatctgaatcaggaagcatatcagaaggacacaagaccttgtgattgctgtggtttgatcactccatctaaagctatgaaaaaagtgaagggctcaggttctgaaacccaagttttatgtaaacattgtgcaaagttgaaaaaatcaagacaatattgtggcatatgtaagaagatatggcaccattcagatggtggagattgggtatgctgtgatggctgtaatgtttgggtgcatgctgagtgtgacaaaatttccagtaaagttttcaaggacccagagcacattgattactattgtcctgattgcaaaaacaaggccaaaggtgaaatgttagatgagttacagtgtcggcaaaaacacagttctggccggaaatctgtgactggcgtaactgtgtctgaggtaactgtggtttgtaatggcatggaaggaacttatgtaccaaaccttcatatggttgcttgtaagtgtggaccctgtgatgagtcaaagatgcagagccttggtgaatgggaaaggcacacaggttgtagagcaaaaaaatggaagcatagtgtgaaagtgaaggccacaatgctaacactggaacaatggattgcagagtataatgaacatggcttacagcctctcaaattagataagcagcgactacttgattccttacaagaaaagtacgagccagttaatgcaaagtggacaagtgaaagatgtgcagtttgtagatgggttgaagattgggaagataacaaaatgatcatatgcaacaggtgtcaaatagctgtgcaccaagaatgctatggtgcaaaagatgtccaggattttacttcatgggtttgtagagcatgtgaaacacctgatatcattaaagaatgttgtctctgtcctgtacaaggaggtgctttgaagcccactgatgttgacaccttgtgggttcatgtctcttgtgcttggtttcgtcccgaagtaggtttcttgaatcatgagaagatggaacctgctgttggaatccttagaatcccacccactacttttatgaagcgttgcgtaatttgtacacagactcatggttcctgcaccacatgttgcaaatgtgccactcattttcatgcaacctgtgcatcaagagcaggctacaacatggaattgcactgccaagagaagaatgggaaacaagtaacaacgaagatgatatattgtgctgtgcacaggacccctaatccagatgctgttgttgttgttcgtactccttcgggggtttttgctgccagaagcttgcttcaaaatgagaaagggttcttcaggggttcgagggtggtctcatcaaagaggactgaagttccagaaccttcaacctcagagtctgatgagttagaaccactctctgctgcaagatgccgtgctattaaaagatcaaattttaagggaggtgagcggctaccaatttttcatcgaccaaggggaccaacacatcattcattagactcagtgaatagcttgagcacatttatagaagtagaagattctaaaattttttcttccttcaaggatcggctttaccacttgcagaaaactgaaaatcaccgggtttgttttggtaaatctggaatacatggatggggtctatttgcgcgtaggaatattcaagaaggagaaatggttgttgagtatcgtggtgagcgggtaagacgtagcgttgcagatctgagggaagcacggtacaggctacaaggcaaagattgctatcttttcaagatcagcgaggaagtagtaattgatgctacatatagaggaaatatagcacgattaattaaccattcgtgcacacccaattgctattcaaggatcatgagtgtgggtgaggagaaccgaatagttctcattgccaagacttgtgtttcggccggtgaagaactaacgtacgattacttgtttgagccggatgaatgtgacgagctgagagttccctgtctatcctctgaagacgcagaagcgtatgcgaagagccaagccgatttgctcttgcacctctcctcgtttcacttcttctggccccgccttcacctggttcgccctctctctgtgaatttgaatttgaatttcgaaaaccggtttcattttgtggatttggattctgtgtactgtgtgtgtgcggttttgcctatgcgagtgtgcgtgaatggacttgcgcaaagagctctttcgtctgtgccgtctggttgccgagaatttaaacagaaacaaaatgtaaaaatgaaagagttcaattcagcagtaaccaaacagagcctaactaattatctgagtttgttggacttgaacagtcacgagcaggacgtttggacggagatcgcaaagtttctggacggaaagtcactggtgatgctcgctgcgacatgtcgttggttccacgcgctggtcgaggacgggagcatatggaggtacttgtgcttgcgtgatcttcaggtccctgctcctcgccccccggcggcgtttgattggaagaagctctacatttccgctttcaatgggagtcactcttacatgttccgccatccggagaagcatatcgtactcctaacagagaggctgagcaccccactgaaattccccaaggaagatagcattcagaaaatgttggagtcgagcggcagttgtgtgttgaacaatgtgaaacctgggatatggattgcagatctccagcttgttcgatgccctgtctgcgagctcaatacttgtgatggaacaatgcaaacattggatgcaaggcatattgaactgttcttgagtgagggataccaggatggtagctgggaatatgaacttattggaattcaggatgtcaaaaagcatgttgagggtgcttctggagctattttcgatgtcaaacacctcaaagactcttcaacttcagcagtcttcaacctcaagtcatgggtcgggaaaccaaatgactggcaaccgaaggctgtgatcacaatgcatgcagttgctgtcaacacaaatctgcagagaaatgaaggtagcctgcaagtcaaataccatgtgatgacagctggagctggaggagaagttgtatctattcgaatctcgcagcagcttctgtag

Protein Analysis

1409

Amino Acids

159.68

Weight (kDa)

8.37

Isoelectric Point (pI)

45.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 242 - 337 1.1e-14 PWWP domain
PHD PF00628 427 - 477 9.5e-07 PHD-finger
PHD_2 PF13831 624 - 658 1.9e-12 PHD-finger
zf-HC5HC2H_2 PF13832 665 - 777 1.1e-25 PHD-zinc-finger like domain
zf-HC5HC2H PF13771 687 - 778 1.8e-10 PHD-like zinc-binding domain
SET PF00856 913 - 1017 7.8e-26 SET domain
F-box-like PF12937 1153 - 1193 8.4e-07 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 682, 3312
Acc36I ACCTGC 1 cut(s) 2128
AccB1I GGYRCC 1 cut(s) 1302
AccB7I CCANNNNNTGG 1 cut(s) 3538
AccBSI CCGCTC 3 cut(s) 2557, 2803, 3771
AccII CGCG 2 cut(s) 2755, 3536
AccIII TCCGGA 1 cut(s) 3679
AclWI GGATC 2 cut(s) 2679, 2975
AcoI YGGCCR 5 cut(s) 14, 296, 767, 1492, 3024
AcuI CTGAAG 6 cut(s) 968, 2407, 2478, 3123, 3569, 4017
AdeI CACNNNGTG 1 cut(s) 2052
AfiI CCNNNNNNNGG 8 cut(s) 1579, 2015, 2090, 2126, 3187, 3538, 3610, 3679
AflIII ACRYGT 3 cut(s) 2204, 3518, 3666
AgeI ACCGGT 1 cut(s) 3231
AhdI GACNNNNNGTC 1 cut(s) 3493
AjnI CCWGG 4 cut(s) 1938, 3186, 3799, 3921
AjuI GAANNNNNNNTTGG 4 cut(s) 2109, 2141, 2581, 2613
Alw21I GWGCWC 8 cut(s) 1407, 1911, 2331, 2627, 2948, 3322, 3719, 3852
Alw26I GTCTC 3 cut(s) 2006, 2068, 2446
Alw44I GTGCAC 3 cut(s) 1907, 2327, 2944
AlwI GGATC 2 cut(s) 2679, 2975
AlwNI CAGNNNCTG 6 cut(s) 1126, 1223, 2351, 2486, 4115, 4225
Ama87I CYCGRG 1 cut(s) 288
Aor13HI TCCGGA 1 cut(s) 3679
ApaLI GTGCAC 3 cut(s) 1907, 2327, 2944
ArsI GACNNNNNNTTYG 2 cut(s) 3004, 3036
AseI ATTAAT 1 cut(s) 2930
AsiGI ACCGGT 1 cut(s) 3231
Asp700I GAANNNNTTC 3 cut(s) 488, 2417, 3383
AspLEI GCGC 4 cut(s) 683, 2755, 3313, 3538
AspS9I GGNCC 7 cut(s) 264, 1396, 1598, 2334, 2585, 3174, 3589
AsuC2I CCSGG 2 cut(s) 2708, 3608
AsuII TTCGAA 3 cut(s) 584, 3225, 4207
AvaI CYCGRG 1 cut(s) 288
AvaII GGWCC 6 cut(s) 264, 1396, 1598, 2334, 2585, 3589
BaeGI GKGCMC 3 cut(s) 1911, 2331, 2948
BaeI ACNNNNGTAYC 2 cut(s) 1548, 1581
BanI GGYRCC 1 cut(s) 1302
BanII GRGCYC 3 cut(s) 1217, 3322, 3852
BarI GAAGNNNNNNTAC 4 cut(s) 1557, 1589, 3617, 3649
BauI CACGAG 1 cut(s) 3449
BbsI GAAGAC 3 cut(s) 303, 3111, 4033
Bbv12I GWGCWC 8 cut(s) 1407, 1911, 2331, 2627, 2948, 3322, 3719, 3852
BbvI GCAGC 9 cut(s) 171, 187, 241, 806, 1046, 1780, 2374, 2497, 3499
BceAI ACGGC 4 cut(s) 292, 311, 2505, 3319
BciT130I CCWGG 4 cut(s) 1940, 3188, 3801, 3923
BciVI GTATCC 2 cut(s) 173, 3911
BclI TGATCA 3 cut(s) 1179, 1878, 4095
BcnI CCSGG 2 cut(s) 2708, 3608
BcoDI GTCTC 3 cut(s) 2006, 2068, 2446
BfmI CTRYAG 2 cut(s) 4130, 4226
BfuAI ACCTGC 1 cut(s) 2128
BfuI GTATCC 2 cut(s) 173, 3911
BglII AGATCT 2 cut(s) 2824, 3817
BlpI GCTNAGC 1 cut(s) 3713
Bme1390I CCNGG 6 cut(s) 1940, 2708, 3188, 3608, 3801, 3923
Bme18I GGWCC 6 cut(s) 264, 1396, 1598, 2334, 2585, 3589
BmeRI GACNNNNNGTC 1 cut(s) 3493
BmeT110I CYCGRG 1 cut(s) 288
BmgT120I GGNCC 7 cut(s) 264, 1396, 1598, 2334, 2585, 3174, 3589
BmrFI CCNGG 6 cut(s) 1940, 2708, 3188, 3608, 3801, 3923
BmrI ACTGGG 2 cut(s) 755, 949
BmsI GCATC 8 cut(s) 1608, 2252, 2338, 2507, 2890, 3495, 3824, 3874
BmuI ACTGGG 2 cut(s) 755, 949
BpiI GAAGAC 3 cut(s) 303, 3111, 4033
BplI GAGNNNNNCTC 2 cut(s) 271, 303
BpmI CTGGAG 6 cut(s) 77, 786, 3806, 4011, 4200, 4206
Bpu10I CCTNAGC 2 cut(s) 72, 1216
Bpu1102I GCTNAGC 1 cut(s) 3713
Bpu14I TTCGAA 3 cut(s) 584, 3225, 4207
BpuEI CTTGAG 4 cut(s) 953, 2641, 3928, 4034
BpuMI CCSGG 2 cut(s) 2708, 3608
BsaBI GATNNNNATC 2 cut(s) 1095, 2865
BsaI GGTCTC 1 cut(s) 2446
BsaJI CCNNGG 5 cut(s) 1627, 2579, 3606, 3735, 3800
BsaWI WCCGGW 3 cut(s) 167, 3231, 3679
Bsc4I CCNNNNNNNGG 8 cut(s) 1579, 2015, 2090, 2126, 3187, 3538, 3610, 3679
Bse118I RCCGGY 3 cut(s) 769, 3026, 3231
Bse1I ACTGG 8 cut(s) 761, 955, 1380, 1513, 1713, 1805, 3504, 4082
Bse3DI GCAATG 2 cut(s) 142, 3003
Bse8I GATNNNNATC 2 cut(s) 1095, 2865
BseAI TCCGGA 1 cut(s) 3679
BseBI CCWGG 4 cut(s) 1940, 3188, 3801, 3923
BseDI CCNNGG 5 cut(s) 1627, 2579, 3606, 3735, 3800
BseGI GGATG 6 cut(s) 2744, 3070, 3676, 3889, 3931, 3967
BseJI GATNNNNATC 2 cut(s) 1095, 2865
BseLI CCNNNNNNNGG 8 cut(s) 1579, 2015, 2090, 2126, 3187, 3538, 3610, 3679
BseMI GCAATG 2 cut(s) 142, 3003
BseNI ACTGG 8 cut(s) 761, 955, 1380, 1513, 1713, 1805, 3504, 4082
BseRI GAGGAG 9 cut(s) 74, 292, 473, 766, 1013, 2999, 3145, 3588, 4203
BseSI GKGCMC 3 cut(s) 1911, 2331, 2948
BseX3I CGGCCG 3 cut(s) 14, 296, 3024
BseXI GCAGC 9 cut(s) 171, 187, 241, 806, 1046, 1780, 2374, 2497, 3499
BseYI CCCAGC 2 cut(s) 635, 3933
BsgI GTGCAG 2 cut(s) 1857, 2182
Bsh1236I CGCG 2 cut(s) 2755, 3536
Bsh1285I CGRYCG 4 cut(s) 17, 207, 299, 3027
BshNI GGYRCC 1 cut(s) 1302
BshTI ACCGGT 1 cut(s) 3231
BsiEI CGRYCG 4 cut(s) 17, 207, 299, 3027
BsiHKAI GWGCWC 8 cut(s) 1407, 1911, 2331, 2627, 2948, 3322, 3719, 3852
BsiHKCI CYCGRG 1 cut(s) 288
BsiSI CCGG 9 cut(s) 168, 770, 1495, 2707, 3027, 3062, 3232, 3608, 3680
BsiWI CGTACG 1 cut(s) 3042
BslFI GGGAC 3 cut(s) 2067, 2598, 3575
BslI CCNNNNNNNGG 8 cut(s) 1579, 2015, 2090, 2126, 3187, 3538, 3610, 3679
BsmAI GTCTC 3 cut(s) 2006, 2068, 2446
BsmFI GGGAC 3 cut(s) 2067, 2598, 3575
BsmI GAATGC 2 cut(s) 1922, 3747
Bso31I GGTCTC 1 cut(s) 2446
BsoBI CYCGRG 1 cut(s) 288
Bsp119I TTCGAA 3 cut(s) 584, 3225, 4207
Bsp1286I GDGCHC 9 cut(s) 1217, 1407, 1911, 2331, 2627, 2948, 3322, 3719, 3852
Bsp13I TCCGGA 1 cut(s) 3679
Bsp1407I TGTACA 3 cut(s) 960, 2011, 2177
Bsp1720I GCTNAGC 1 cut(s) 3713
BspEI TCCGGA 1 cut(s) 3679
BspFNI CGCG 2 cut(s) 2755, 3536
BspHI TCATGA 2 cut(s) 2104, 2970
BspMAI CTGCAG 1 cut(s) 4134
BspMI ACCTGC 1 cut(s) 2128
BspPI GGATC 2 cut(s) 2679, 2975
BspQI GCTCTTC 1 cut(s) 3119
BspT104I TTCGAA 3 cut(s) 584, 3225, 4207
BspT107I GGYRCC 1 cut(s) 1302
BspTNI GGTCTC 1 cut(s) 2446
BsrBI CCGCTC 3 cut(s) 2557, 2803, 3771
BsrDI GCAATG 2 cut(s) 142, 3003
BsrFI RCCGGY 3 cut(s) 769, 3026, 3231
BsrGI TGTACA 3 cut(s) 960, 2011, 2177
BsrI ACTGG 8 cut(s) 761, 955, 1380, 1513, 1713, 1805, 3504, 4082
BssAI RCCGGY 3 cut(s) 769, 3026, 3231
BssECI CCNNGG 5 cut(s) 1627, 2579, 3606, 3735, 3800
BssSI CACGAG 1 cut(s) 3449
BssT1I CCWWGG 3 cut(s) 1627, 2579, 3735
Bst2BI CACGAG 1 cut(s) 3449
Bst2UI CCWGG 4 cut(s) 1940, 3188, 3801, 3923
Bst6I CTCTTC 3 cut(s) 95, 3119, 4030
BstAPI GCANNNNNTGC 2 cut(s) 2240, 2862
BstAUI TGTACA 3 cut(s) 960, 2011, 2177
BstBI TTCGAA 3 cut(s) 584, 3225, 4207
BstENI CCTNNNNNAGG 1 cut(s) 2013
BstF5I GGATG 6 cut(s) 2744, 3070, 3676, 3889, 3931, 3967
BstFNI CGCG 2 cut(s) 2755, 3536
BstHHI GCGC 4 cut(s) 683, 2755, 3313, 3538
BstMAI GTCTC 3 cut(s) 2006, 2068, 2446
BstMCI CGRYCG 4 cut(s) 17, 207, 299, 3027
BstNI CCWGG 4 cut(s) 1940, 3188, 3801, 3923
BstNSI RCATGY 7 cut(s) 1361, 1971, 2208, 3522, 3670, 3977, 4109
BstSCI CCNGG 6 cut(s) 1938, 2706, 3186, 3606, 3799, 3921
BstSFI CTRYAG 2 cut(s) 4130, 4226
BstSLI GKGCMC 3 cut(s) 1911, 2331, 2948
BstUI CGCG 2 cut(s) 2755, 3536
BstV1I GCAGC 9 cut(s) 171, 187, 241, 806, 1046, 1780, 2374, 2497, 3499
BstV2I GAAGAC 3 cut(s) 303, 3111, 4033
BstX2I RGATCY 2 cut(s) 2824, 3817
BstXI CCANNNNNNTGG 3 cut(s) 500, 1313, 4077
BstYI RGATCY 2 cut(s) 2824, 3817
BstZI CGGCCG 3 cut(s) 14, 296, 3024
BsuI GTATCC 2 cut(s) 173, 3911
BtsCI GGATG 6 cut(s) 2744, 3070, 3676, 3889, 3931, 3967
BtsI GCAGTG 3 cut(s) 411, 816, 2272
BtsIMutI CAGTG 9 cut(s) 411, 816, 1475, 1706, 2034, 2272, 2616, 3497, 3722
BveI ACCTGC 1 cut(s) 2128
CaiI CAGNNNCTG 6 cut(s) 1126, 1223, 2351, 2486, 4115, 4225
CciI TCATGA 2 cut(s) 2104, 2970
CfoI GCGC 4 cut(s) 683, 2755, 3313, 3538
Cfr10I RCCGGY 3 cut(s) 769, 3026, 3231
Cfr13I GGNCC 7 cut(s) 264, 1396, 1598, 2334, 2585, 3174, 3589
CseI GACGC 1 cut(s) 3116
CsiI ACCWGGT 1 cut(s) 3186
CspAI ACCGGT 1 cut(s) 3231
CspCI CAANNNNNGTGG 2 cut(s) 2023, 2058
DraI TTTAAA 1 cut(s) 3355
DraIII CACNNNGTG 1 cut(s) 2052
DriI GACNNNNNGTC 1 cut(s) 3493
EaeI YGGCCR 5 cut(s) 14, 296, 767, 1492, 3024
EagI CGGCCG 3 cut(s) 14, 296, 3024
Eam1104I CTCTTC 3 cut(s) 95, 3119, 4030
Eam1105I GACNNNNNGTC 1 cut(s) 3493
EarI CTCTTC 3 cut(s) 95, 3119, 4030
EciI GGCGGA 3 cut(s) 80, 121, 3662
Ecl136II GAGCTC 2 cut(s) 3320, 3850
EclXI CGGCCG 3 cut(s) 14, 296, 3024
Eco130I CCWWGG 3 cut(s) 1627, 2579, 3735
Eco147I AGGCCT 1 cut(s) 77
Eco24I GRGCYC 3 cut(s) 1217, 3322, 3852
Eco31I GGTCTC 1 cut(s) 2446
Eco32I GATATC 1 cut(s) 1984
Eco47I GGWCC 6 cut(s) 264, 1396, 1598, 2334, 2585, 3589
Eco52I CGGCCG 3 cut(s) 14, 296, 3024
Eco53kI GAGCTC 2 cut(s) 3320, 3850
Eco57I CTGAAG 6 cut(s) 968, 2407, 2478, 3123, 3569, 4017
Eco88I CYCGRG 1 cut(s) 288
EcoICRI GAGCTC 2 cut(s) 3320, 3850
EcoNI CCTNNNNNAGG 1 cut(s) 2013
EcoO109I RGGNCCY 3 cut(s) 1396, 2334, 3589
EcoRI GAATTC 1 cut(s) 3954
EcoRII CCWGG 4 cut(s) 1938, 3186, 3799, 3921
EcoRV GATATC 1 cut(s) 1984
EcoT14I CCWWGG 3 cut(s) 1627, 2579, 3735
EcoT22I ATGCAT 1 cut(s) 4107
EcoT38I GRGCYC 3 cut(s) 1217, 3322, 3852
ErhI CCWWGG 3 cut(s) 1627, 2579, 3735
FaqI GGGAC 3 cut(s) 2067, 2598, 3575
FauI CCCGC 2 cut(s) 2796, 3185
FauNDI CATATG 4 cut(s) 1286, 1578, 1883, 3557
FbaI TGATCA 3 cut(s) 1179, 1878, 4095
FokI GGATG 6 cut(s) 2751, 3077, 3663, 3896, 3938, 3974
FriOI GRGCYC 3 cut(s) 1217, 3322, 3852
FspI TGCGCA 2 cut(s) 682, 3312
GlaI GCGC 4 cut(s) 682, 2754, 3312, 3537
GsaI CCCAGC 2 cut(s) 639, 3937
GsuI CTGGAG 6 cut(s) 77, 786, 3806, 4011, 4200, 4206
HapII CCGG 9 cut(s) 168, 770, 1495, 2707, 3027, 3062, 3232, 3608, 3680
HgaI GACGC 1 cut(s) 3116
HhaI GCGC 4 cut(s) 683, 2755, 3313, 3538
Hin6I GCGC 4 cut(s) 681, 2753, 3311, 3536
HinP1I GCGC 4 cut(s) 681, 2753, 3311, 3536
HincII GTYRAC 3 cut(s) 667, 2044, 4120
HindII GTYRAC 3 cut(s) 667, 2044, 4120
HindIII AAGCTT 1 cut(s) 2395
HpaII CCGG 9 cut(s) 168, 770, 1495, 2707, 3027, 3062, 3232, 3608, 3680
Hpy99I CGWCG 3 cut(s) 22, 178, 188
HpyCH4IV ACGT 4 cut(s) 589, 2812, 3041, 3459
HpySE526I ACGT 4 cut(s) 589, 2812, 3041, 3459
HspAI GCGC 4 cut(s) 681, 2753, 3311, 3536
Kpn2I TCCGGA 1 cut(s) 3679
KroI GCCGGC 1 cut(s) 769
KroNI GCCGGC 1 cut(s) 771
Ksp22I TGATCA 3 cut(s) 1179, 1878, 4095
LguI GCTCTTC 1 cut(s) 3119
LmnI GCTCC 8 cut(s) 170, 632, 753, 805, 3552, 3601, 3992, 4181
Lsp1109I GCAGC 9 cut(s) 171, 187, 241, 806, 1046, 1780, 2374, 2497, 3499
LweI GCATC 8 cut(s) 1608, 2252, 2338, 2507, 2890, 3495, 3824, 3874
MabI ACCWGGT 1 cut(s) 3186
MaeII ACGT 4 cut(s) 589, 2812, 3041, 3459
MbiI CCGCTC 3 cut(s) 2557, 2803, 3771
MfeI CAATTG 2 cut(s) 676, 2953
MflI RGATCY 2 cut(s) 2824, 3817
MhlI GDGCHC 9 cut(s) 1217, 1407, 1911, 2331, 2627, 2948, 3322, 3719, 3852
MlyI GAGTC 9 cut(s) 197, 227, 1619, 2179, 2492, 2600, 3664, 3773, 4016
MmeI TCCRAC 3 cut(s) 2107, 3414, 3741
Mph1103I ATGCAT 1 cut(s) 4107
MroI TCCGGA 1 cut(s) 3679
MroNI GCCGGC 1 cut(s) 769
MroXI GAANNNNTTC 3 cut(s) 488, 2417, 3383
MslI CAYNNNNRTG 3 cut(s) 1311, 2229, 2975
MspA1I CMGCKG 3 cut(s) 162, 232, 4178
MspI CCGG 9 cut(s) 168, 770, 1495, 2707, 3027, 3062, 3232, 3608, 3680
MspR9I CCNGG 6 cut(s) 1940, 2708, 3188, 3608, 3801, 3923
MunI CAATTG 2 cut(s) 676, 2953
Mva1269I GAATGC 2 cut(s) 1922, 3747
MvaI CCWGG 4 cut(s) 1940, 3188, 3801, 3923
MvnI CGCG 2 cut(s) 2755, 3536
NaeI GCCGGC 1 cut(s) 771
NciI CCSGG 2 cut(s) 2708, 3608
NdeI CATATG 4 cut(s) 1286, 1578, 1883, 3557
NgoMIV GCCGGC 1 cut(s) 769
NmeAIII GCCGAG 1 cut(s) 3371
NmuCI GTSAC 6 cut(s) 1367, 1504, 3071, 3446, 3495, 3656
NsbI TGCGCA 2 cut(s) 682, 3312
NsiI ATGCAT 1 cut(s) 4107
NspI RCATGY 7 cut(s) 1361, 1971, 2208, 3522, 3670, 3977, 4109
NspV TTCGAA 3 cut(s) 584, 3225, 4207
PacI TTAATTAA 1 cut(s) 2934
PaeI GCATGC 2 cut(s) 1361, 4109
PaeR7I CTCGAG 1 cut(s) 288
PagI TCATGA 2 cut(s) 2104, 2970
PceI AGGCCT 1 cut(s) 77
PciI ACATGT 3 cut(s) 2204, 3518, 3666
PciSI GCTCTTC 1 cut(s) 3119
PcsI WCGNNNNNNNCGW 5 cut(s) 95, 581, 3332, 3456, 3540
PctI GAATGC 2 cut(s) 1922, 3747
PdiI GCCGGC 1 cut(s) 771
PdmI GAANNNNTTC 3 cut(s) 488, 2417, 3383
Pfl23II CGTACG 1 cut(s) 3042
PflMI CCANNNNNTGG 1 cut(s) 3538
PfoI TCCNGGA 1 cut(s) 1938
PinAI ACCGGT 1 cut(s) 3231
PleI GAGTC 9 cut(s) 196, 226, 1618, 2179, 2491, 2600, 3663, 3772, 4016
PpsI GAGTC 9 cut(s) 196, 226, 1618, 2179, 2491, 2600, 3663, 3772, 4016
PpuMI RGGWCCY 3 cut(s) 1396, 2334, 3589
PscI ACATGT 3 cut(s) 2204, 3518, 3666
PshBI ATTAAT 1 cut(s) 2930
Psp124BI GAGCTC 2 cut(s) 3322, 3852
Psp5II RGGWCCY 3 cut(s) 1396, 2334, 3589
Psp6I CCWGG 4 cut(s) 1938, 3186, 3799, 3921
PspFI CCCAGC 2 cut(s) 635, 3933
PspGI CCWGG 4 cut(s) 1938, 3186, 3799, 3921
PspLI CGTACG 1 cut(s) 3042
PspPI GGNCC 7 cut(s) 264, 1396, 1598, 2334, 2585, 3174, 3589
PspPPI RGGWCCY 3 cut(s) 1396, 2334, 3589
PspXI VCTCGAGB 1 cut(s) 288
PsrI GAACNNNNNNTAC 2 cut(s) 1728, 1760
PstI CTGCAG 1 cut(s) 4134
PstNI CAGNNNCTG 6 cut(s) 1126, 1223, 2351, 2486, 4115, 4225
PsuI RGATCY 2 cut(s) 2824, 3817
PvuII CAGCTG 1 cut(s) 4178
RseI CAYNNNNRTG 3 cut(s) 1311, 2229, 2975
SacI GAGCTC 2 cut(s) 3322, 3852
SapI GCTCTTC 1 cut(s) 3119
Sau96I GGNCC 7 cut(s) 264, 1396, 1598, 2334, 2585, 3174, 3589
SchI GAGTC 9 cut(s) 197, 227, 1619, 2179, 2492, 2600, 3664, 3773, 4016
ScrFI CCNGG 6 cut(s) 1940, 2708, 3188, 3608, 3801, 3923
SduI GDGCHC 9 cut(s) 1217, 1407, 1911, 2331, 2627, 2948, 3322, 3719, 3852
SexAI ACCWGGT 1 cut(s) 3186
SfaNI GCATC 8 cut(s) 1608, 2252, 2338, 2507, 2890, 3495, 3824, 3874
SfcI CTRYAG 2 cut(s) 4130, 4226
Sfr274I CTCGAG 1 cut(s) 288
SfuI TTCGAA 3 cut(s) 584, 3225, 4207
SinI GGWCC 6 cut(s) 264, 1396, 1598, 2334, 2585, 3589
SlaI CTCGAG 1 cut(s) 288
SmiMI CAYNNNNRTG 3 cut(s) 1311, 2229, 2975
SmlI CTYRAG 5 cut(s) 288, 932, 2620, 3907, 4049
SmoI CTYRAG 5 cut(s) 288, 932, 2620, 3907, 4049
SphI GCATGC 2 cut(s) 1361, 4109
SseBI AGGCCT 1 cut(s) 77
SspI AATATT 3 cut(s) 497, 1277, 2764
SstI GAGCTC 2 cut(s) 3322, 3852
StuI AGGCCT 1 cut(s) 77
StyD4I CCNGG 6 cut(s) 1938, 2706, 3186, 3606, 3799, 3921
StyI CCWWGG 3 cut(s) 1627, 2579, 3735
TaiI ACGT 4 cut(s) 592, 2815, 3044, 3462
TatI WGTACW 4 cut(s) 960, 2011, 2177, 3261
TauI GCSGC 9 cut(s) 16, 165, 247, 301, 654, 709, 2560, 3614, 3774
TscAI CASTG 9 cut(s) 411, 816, 1475, 1713, 2041, 2279, 2616, 3504, 3729
TseFI GTSAC 6 cut(s) 1367, 1504, 3071, 3446, 3495, 3656
Tsp45I GTSAC 6 cut(s) 1367, 1504, 3071, 3446, 3495, 3656
TspGWI ACGGA 3 cut(s) 906, 3482, 3504
TspRI CASTG 9 cut(s) 411, 816, 1475, 1713, 2041, 2279, 2616, 3504, 3729
Van91I CCANNNNNTGG 1 cut(s) 3538
VneI GTGCAC 3 cut(s) 1907, 2327, 2944
VpaK11BI GGWCC 6 cut(s) 264, 1396, 1598, 2334, 2585, 3589
VspI ATTAAT 1 cut(s) 2930
XagI CCTNNNNNAGG 1 cut(s) 2013
XceI RCATGY 7 cut(s) 1361, 1971, 2208, 3522, 3670, 3977, 4109
XcmI CCANNNNNNNNNTGG 3 cut(s) 1313, 1919, 2285
XhoI CTCGAG 1 cut(s) 288
XmnI GAANNNNTTC 3 cut(s) 488, 2417, 3383
Zsp2I ATGCAT 1 cut(s) 4107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.