Rmu_ssc0000410.1_g000046

Nucleolar pre-ribosomal-associated protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000410.1
OG0002923
Physical Location & Seq
Reverse (-)
277853 .. 305229
27377 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 11022 bp
atgattttgggcttcagccctaatcctttgggcttttcggcccaacacttaaacccttggtatgcttgctctctaccgcccgtcttaaaccctaaaatcaccaaaacctcactgactgtttttgcaatggaagaaggaagctcagcctgcgaggatcaagttatggtggaggaagcctatgacagtgaggttatgggagaagaattgccgaaagttgagcttaaggagaatcatgaagcgaaagttaaagaactattgcataagattacttctaatgagattaaattatgcgctgccggtgcgaaagagtttataaagttgctgaaaggtgattctggaggtgaattgctgtattactatgtgcaaaattcagctcagtgctcggagcttctgggtgcgtggaatctccgtcgagggaagccggggatgttttatatagttaagttgatttctgttgttttgagtcatcctgatggggtttatgaagcaaatgatatggggaggttgggggtgagtagggtgttggacaagtttgcgcggtcgattattgagcaacagttgggagatgtgcataaggagttgaagagtagtgaagctaagcgccagaaggcggcgctgttgcttttggcttcgattgtgaggcgtggatcggtgttggcgtctgaggtggaaaagaagtttgatttcaagctgcaggggttttgcaagttgggggagtataaaatgaggccaaatgagaagaggaggaagctgtcattgaggaagttgtttgttgggtttgcaatgtcgtttctggaggtgggagcgccgggattgttgaggtcggttatacggcagaaggaaatgtattcttgtgtcctccgctggctggggaatgatgatgaggagacagttatatatgttctgtccacattgcagaacaggatacttgttgagggttcatcggttccccctgcccttcggagtgttctgtttgggagtggtactttagaacaattagccaatatttctggaagggagaatggtggagcttctgcagaattggcatacagggtcttagttatggtttgcaccgatccatctaatggcctaatggcagattcaaagagacatctgaagggcaatttaaagcggcaaatagacctaatgaagaagcttaaggcgaccgagattggctaccacagggatctgcttttggctgttgttagcgggagaccgtctcttggtgcagcatacatggaagagttcccttacaatcttgaagattatgcatcacccaaatggttttctgttgttactctagcagcaaacttggtttcctctgtgggcagtgggatcccagccttgtttgacaatgttcatgtgcaggatgtcatgaaatgcttgtatcctcgtcagttcaaccgctccatgatcaataaagggctgcttcactctgattttctagtgaagcatggaactttaaggcatcttttagaggcactcaagttgttagattccttccttggtgttttaaactgtagagaccaacatgtttcggaatctctcaagcaagaatttcaaaatgaagttcgaagcttgctcccagatcctcaactttttaagactctactctcttctatgagtagcaactcaagaaagaggactgcggatctggagaagttcccagaacatagcccgaagaaagtgaaaaaattgaaaacagattttggaaacaaggactcagatattgttgtaggcggaataagttttggtccagatacgtccgaaaatgaagatagtcttgtgaatgttatggcagatctttggggtttcgatccttgctccacacctattactgcactcaaggatgcagacttatatttctactgtaggcggataatgcccactggcttggagggctcatttgagttccttatgaagctcctaagcagtcctttagcattacaaaataatctgcagtgctctcttttgtctttgctaatagaatacattggatggtctccaaggaatagaacaccaattaagactccacccttgatgtacaaacatctacagacattgatgaagttgctgattttttcaccactcagtgacataaaagatcaagcatactgtctcacacaagcagctatgttaagtactggtgcttttgacaaaaaccaacatgaaattgcatcatggttcttgtttataccgggttttgatagaggaaaatcttcggttgaagtcctgggggtgggagtattacaaagcttggctaatgctgtcatctcattcttatctgatgccgtttctactacagggaagaatttcttcaagcattgggatcttattgttaagcgtgataactaccatttggaaactgttaaagatgtgtcacctcaggcccctctaatcttacgggtactgcagaattgtctgagattgcttgattctggatctgggacttttacattacctgagaagacaatgatatctacctatgtgtgcaatacactgaaatatatcttgaaaactcaggttgatgcacagtttctatcttctatgatttgctcagtcttggttgagagacttggtgactgttctgccactcatcagtctaggggcaactgtgaatggaggccgttgaacagtttatttttgttttcccagagtatatctgaccgtgaaatttgtttcatttcttctattgacaataaggctaagccttctggtagttcttttgcactagcgcttgaggaagttggaagacttgcaagaggtgggagtgatggtgaagtagctggaataaccaaagccttcttctcctcatttatctgtacaacgcctgatgaaatattagcaaactttccatcagttatggcaatttcacaatgccttggtcttccattcactctcttttcatcaattttctttcttgaacccacctttctccccagagtttctaagttttggcctgaagttttttttcctgggcttagtatggctctaaccaatatctgttgcaaaggaggaaaagaaattgcctgtggatctcctgatcatgcttcttatacaggagaagcaatatgtaaccaaaattgtggtgcaaatgaagctgctgctactgccttcagtttatttttgatgaaggcacctttccacgttttgttccctgccattatgttcattgacggtccttattcgttagagccctcaaagatacaagacctgcttttggctaaactatcagactgtgcaactgattaccaccttatttcctatttgcgccttgtgttattttggctttatcagatacggttgtcctgtacagttgaacaattagtcggtttccaacaactttctgagatttgctcagttctggtgaagaacttattatctcaactattggtggctgactctgattccctcagaacctccagtgtaaatctatcaagtcatgatattcaaaaggtggctgaaactatcttttatcatcctgcaataactacatcgttatcctgtcctttggagtgtagcgaagacttaccagagggtaacctaccagacaacgtggatgctctcatcaatttatcgagggggaaaggtcatagattagaccatcatgccttggatatattggcaacaacttgcgagtacttgttttctctgtgcaatgacaaccaatttacacttgaagtagaagatggggatgctacaaagtttgtgaaagcattcaacgtcttggttcataagctctttcaggaagtcaaggtcaactttgatcacagcattagaagtaacaattgtatggctttcctcccaacattttatgctttgcatgctttgagtggttttatatctccatttgagctacttgaattagtgcgttggatgtttagtagagttgatttggatggcaaccagaaatctgctatttgttttggatcttttattgctggtggtgcatttagaaatctatcttattatttgcagcagccaaatacaaagagaaaggcatttgatttgttttggacgatggaagaaaacaatattaatagcgacattgttgaagaaatctatattaagatatcaaagcttgcctttcttttggaatcagaagttttagatcattgcttgctggaagctatgaatgtggtatacaggcagaaatgtatgcaaaagtgtaattttcatccattaataatagtgatgtcgagagttatagcaaccacgccaatagaaatgctttcccattgcatttacagaacaagcaagatgaaagctagattgttgtctcttcttattgatctgagctctatgcatttgtctacctttgggcacttactttttggtactgtagatactagttgtctacataatggcaatgtgacatctgacctagctctttcagatgacgactatatgatgcttctgccttctgctgtgtcctacatgaattcagttttaatgaaatttggagagccatgctacaggcattttagaaatattccttccttttattcaaagattattttggatggtctccttcattggaagaattttgtttctcgtgatgtgtttcatgaagattttggagaggttttgccgtcatctgctgaagaagttcttaacctcattaatgatagtcttcttggcaaagcgatttgtatgctgcgctatcactatgctttgaatgcagattcagtagaaaggaaagaccgattaaagcatttcagttccattttcccagcttctgtgggacacaaggagctgatagactgggatgttggtggattgggttctcactctttcaatgaaacattaaaccttgtaaataaaattcatgcaaaggtctcgttttgtaggatgctgttattccccaaagatactcaggttcactctctttcaattgaagccagtgggaacctgaaagacgttcctctggacatggaagataacaaaggtaactcctcacaaatgcagtttttgaacatcttggtggatatgtggcaatgcattgttaaaacagtcccttcagtctttcatagttctcaaaagggacacagcacagatagttcttcattgtggagatacctggaagttctgatattgaaaagcatttttgaagtgtctagagagatgcatgacagtctaatcagagtacaatctatacccttcctagagaaattgatgaaaccagctcttctgcataggtttgacgatccaacaactttgcaaatgctgcgtgatcttttgacttttctatctggagggaaattttcgcgtgttccctatcttcaccttttacttgcacactctcaatttgtgcctactattcgttcactatccaaaccatcacattcttcccatgtgggtgccttttctaggcctatgtctagcattcttagatcacctgtttcccttacctcaaaacaaaatgtagatgatggggaatgcgatctagaaacatctgagctgtatgttaaacagttggaggttatcaaattactgcagacactccttcctttgaggtttcaacaggatggttttgactttggaaaagatttaggcattgatcttagggaagttcatttactacttttgtcttcttatggtgcaacactttctgaaattgatgtggagatatacaatctgatgcgcacaattgagtgtattgatggtttggagcatgtcctatttgccggaatggattacctttggggcagtgctgcgttgaagatagaaaaggaacggaatctggagcagagtttgtcttatgatacaatcaatgatgctgaagcagtaaaagaacaccgtagaaatcaagttagagaaaatcttgcgattgaccccaaaatatgcgcgtccactttgctatattttccctaccatatagtggcatctgatgagctattatccttgaacaagtttcaaacagatcctgttgatcatatgcatgtgttatgctcccccgatgttgacgctaaagaaagatataatcctattttcatcttgcgcttctctatgcattgtctttcagaaggttacattgaacccttggagtttgctggtttaggcttacttgctattgcctttatgagtatttcatcagctaatgacaagataagaagtttgggttatgaaactcttgaaacattgcaggacaagttgaaggtggtcaatgatgttgtttcttcaagaaacattactgaatggttgcagaacattgccctcgagcagctcatggagttcacatctcatctctacaaatttcttgcgactgatgtgactttgataacagataatgttgcagtagtcaatcaaattcttaaaacaataatatctacattcaagagtggtgaaaagctgtcaagttttatcatgtgggcaatttccagtgctgtagaagcagactctgcaactatgcttcattccaatgaatctcatcaatgtttcactactattccagaggaaaaagaggtgtataagaattctttgatttcaaaactattgcgttggctaactgcaactgtaattcttgggaagcttgactggacattcagtgacatggatcctgagttctcgaaaagtttgaatttaaaaagtttgcactctttaataactcatgctgacaaggcatgtggagaaaggggccgaagcagatatggttgtgaagagatcttggcatcagcaattctctaccttcaacagctctctggaacaaattacgaaatgctaccatctgtcaatgccgcccttagtcttctcctttccaatgcctccatttgtgcaggtttcttgcatgataatgaaactgtccaatcactttggcagaacatacgttgccctaatgaagctaatcctgcctggagatggtcatttgaccagccatggaaggatccgatgttggaagtcagtgattcccagaagatggaggaactccatgcttatgattccactgctgagatattggatagaaattttctgtcaatgttggacaaggatactttgacttctttttcttctgaggaattctatgaggaagtgaaaatggaaaatgcaaaagagagatcaggttacaggtcttcacagaaggctgctattaaggcttccaaaaaaccaaagaattctgatgctgcacatcaggaaatcatggctctggaagttgtgggtggagcttttctctcttctgctcttgctgttctgtttgataggatggcttcccgtccggttgttgacttcatccgaggaaagaagaccaccgctgggctgctccaccagttgaagatgaagttgctgtcggttaacaagttgctcgatgatgcagaggagaagcaactcagaaactctagagtcagggattggcttaatgagcttaaagacgttctctatcatgccgatgaccttttggatgaaatcaagactgaagctctgcgttgtaagatggaacaagaagattctggaagcagcagcacatatcaggtactcaagtttagttctacttccattcatgaacttgacgaaagtctggaacccaggattctggacattcttgacagattagaagttattgtcaacgaaaaagatgtgcttgatttgaaagagggtgttaaagatagaccacaaggaagactgcccacaacttctttggtagaagagtccagtgtatatggaagggatgaagaaaaggaggccataatcaagctattgttagcggatggtatgactggaaataagatagatgtgcttccaattgtgggtatgggtgggcttggaaagaccacccttgctcaactcacatacaatgatgacagagtcgagcaacattttgaccatcggtcatggttttgtgtttctgaaggatttgatgttaccaaaataacacagtacatttacgggtcgttgacttcagcagtttgtaatatcacagacctagacattcttcaagttaaactaaaggagatgctgtcagggaagaaatttctctttgttcttgatgatgtttggactgtgactaatgacgagtgggatctcttaagccgaccctttgagagtggaggtcatggaagtaagatcattgtcacaacacgtcatgaagatgttgcaggcattgtaggtacccttcaaagtcaacatctaatgcaactatctgaggaaaactgctggtcgttgttctcaaaacatgccttcaagaatggaggtgttactgcagatctgtcgcttgaagtgattggaaaacagattgctcaaaagtgtaaaggactccctttagctgcaaaatcacttgggggtctgttactttctgaaccaaatattgagaaatgggaagaagtgttgaacaatgacatatgggagttgctagataaaaagaatggcattttgccagctctatggttgagctaccattatctacctccgcatctgaagcgttgttttgcttactgttccatatttcctcaagattatctgtttcagaaatcggatctggtgttgctgtggatggctgaagatctcttaccgtcgaaatcgaagactacattagaacaagtcggagagaactactttgatgatctagtgtcaaggtctttttttcaacctataccagatttggttgagccaattttcacaatgcatgaccttatccatgacttagcaaactttgtctctggagagttctgtgttaggttggagaacaatgatgaggtgttggaaaatgtcagcaagagtcgccactttttgtgtatggatgcatctgccgcaattgatcaatgggacagtttgtgtgaagccaagtatttgcgaacctttctagtgtcaacacatcggtacccacgtcccaacttagattttgatatattcctaaagcttcagtgtttaagagtgctcgatttatcaaggtacgaaattggtgagctgcctagttcaattagcaagttgaagcaactaaggcactttgacttgtctggcacatcaattaaaaagttaccggatgaaatgtgtactttatataatttacagacgttgttgttgttcgcttgtgcatctctagttgagttgccaaatgacttgggaagtttaatcaacttgcgtcatctggatattagaggcacaaagatggaaaagatgccaccaaggatgggcaaaatgaaagatctccaaacaataagaggaggtaagtttgtcctagacaaatacactggtggtaacattgtcgagattaaagagcttcagcagttgcgcggaacactatgcatctcggggcttcgcaatattgcgcatgtcacagatgccttggagaccaatatcaggaagaagaagtatttggatgaattagttttggaatggggaggcaagtctggtgacaccgacgatacgcaaaaagatagagatgtgctggagaacctccaacctcatacaaacctaagaaaactcaagattcgatcttatggcggcacaagatttccaggttggttggaagatctttctagtttatctaatttggtccatcttcgacttctggattgcggaaattgtttgtccttgccatcagttgagcagctaccctccctccaagtgctttcgattcaaggatttaatggagtggtgactgtgggttctaagccttttcctaatctacgtgagcttcgtctgaaatcttgtcccgaggtaaccggggcattcatgttagcctgcttcccaaagcttgaaagcctcgatttggaggatgtgaatgtagaatctcttcattccactacactactcctctccctcgtcaaactcagaatatcttcatgcccaaattttgtttgttttcaagatggagggctgtatgcgcccaacttggagaccatcgaagtccataaatgttcgaaactgaggtcattgccgcaacacatgcacaaccttctcccatctctctggttgttctttgtagatgattgtccagaattggaatcatttcccgaagggggattgccctccgtagctcaaggagaaagcacttcatatagttatatgatctataagtctggactgatacaccacactgtcattggtcctcttgaacatgatacagtttacttctacaaatgtggaggacaaggtcctgagtttcagctcaagacccctgcagctcagtttccaattagttttgctgtggccggagatcttggtcaaactggatggacaaagtcaaccctagaccacatcgagcgctgcaagtatgatgttcatatccttcctggagacctttcatatgctgattatttgcagcctcggtgggatacctttggtgagctggttcagccactcgcaagtgcaaggccatggatggtaacccagggaaaccatgagaaggagagcataccattcttgaaggatgggtttgaatcctataattctaggtggaaaatgccgtataaagagagtggatcgagctcgaatctttattactcctttgaagttgcaggtgctcatattatcatgcttggctcgtatactgattatgatgaatattcagatcaatacaagtggttgaaggctgatctgtcaaaggtagaccgacaaaagacaccttggttgcttgtggtatttcatgttccctggtataatagtaacaaggctcatcaaggtgaaggggacagcatgatggcagcaatggagccattgctttatgctgctagtgctgatatagtgtttgctggtcatgttcacgcttatgaacgctcgaaccgtgttaacagtggaaaatcaaatacatgtggtgcagtccacattaccattggtgatggaggaaatagagaaggcttagctcaaaggtttgagcacccctcacctgagtggtcagttttccgggaagcaagttttggtcacggtgagctagagatagtcaattcaacccatgccttttggacctggcataggaatgatgatgatgagcctgtaaggtctgatcaggtatggatgacctccttggtcagttcgggatgtgttgctgaaaagagacatgagctgaggaagatactcatatcaccatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NopRA1 Npa1

Protein Analysis

3673

Amino Acids

413.22

Weight (kDa)

5.79

Isoelectric Point (pI)

45.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Npa1 PF11707 127 - 430 6.8e-43 Ribosome 60S biogenesis N-terminal
Rx_N PF18052 2389 - 2480 2e-17 Rx N-terminal domain
NB-ARC PF00931 2556 - 2727 6.4e-37 NB-ARC domain
WHD_DRP PF23559 2812 - 2880 2e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 2950 - 3245 1.7e-30 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 3065 - 3192 1.4e-39 R13L1/DRL21 LRRs
Metallophos PF00149 3378 - 3568 2.3e-17 Calcineurin-like phosphoesterase
Metallophos_C PF14008 3584 - 3640 1.5e-19 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 314
AarI CACCTGC 1 cut(s) 10442
Acc16I TGCGCA 2 cut(s) 5645, 9261
Acc36I ACCTGC 3 cut(s) 3222, 6767, 10442
Acc65I GGTACC 2 cut(s) 8102, 8811
AccB1I GGYRCC 4 cut(s) 3136, 5360, 8102, 8811
AccB7I CCANNNNNTGG 3 cut(s) 1085, 5881, 6916
AccBSI CCGCTC 1 cut(s) 1407
AccI GTMKAC 5 cut(s) 4143, 4313, 4357, 10481, 10542
AccII CGCG 4 cut(s) 538, 5269, 5849, 9225
AcoI YGGCCR 1 cut(s) 10143
AcyI GRCGYC 1 cut(s) 659
AdeI CACNNNGTG 1 cut(s) 2093
AfeI AGCGCT 2 cut(s) 2736, 10199
AflII CTTAAG 3 cut(s) 221, 1157, 8020
AflIII ACRYGT 3 cut(s) 1531, 8072, 10742
AhdI GACNNNNNGTC 1 cut(s) 7822
AhlI ACTAGT 1 cut(s) 4349
AjiI CACGTC 2 cut(s) 8075, 8819
AjuI GAANNNNNNNTTGG 6 cut(s) 2789, 2821, 9290, 9322, 10833, 10865
AleI CACNNNNGTG 1 cut(s) 10822
AloI GAACNNNNNNTCC 2 cut(s) 6548, 6580
Alw21I GWGCWC 7 cut(s) 383, 1967, 4301, 8871, 10424, 10459, 10812
AlwNI CAGNNNCTG 3 cut(s) 4739, 5927, 6401
Ama87I CYCGRG 3 cut(s) 6218, 9241, 9647
Aor51HI AGCGCT 2 cut(s) 2736, 10199
ArsI GACNNNNNNTTYG 2 cut(s) 9848, 9880
AseI ATTAAT 3 cut(s) 4038, 4184, 4623
Asp700I GAANNNNTTC 8 cut(s) 2218, 2312, 2315, 3716, 4608, 9726, 9772, 9942
Asp718I GGTACC 2 cut(s) 8102, 8811
AspS9I GGNCC 9 cut(s) 40, 1754, 2389, 3179, 6637, 9487, 10040, 10088, 10896
AsuC2I CCSGG 5 cut(s) 423, 810, 2199, 9658, 10838
AsuII TTCGAA 2 cut(s) 1573, 9854
AvaI CYCGRG 3 cut(s) 6218, 9241, 9647
AvaII GGWCC 6 cut(s) 1754, 3179, 9487, 10040, 10088, 10896
AxyI CCTNAGG 1 cut(s) 2385
BaeGI GKGCMC 1 cut(s) 4326
BamHI GGATCC 3 cut(s) 1335, 6556, 6883
BanI GGYRCC 4 cut(s) 3136, 5360, 8102, 8811
BanII GRGCYC 4 cut(s) 1904, 3198, 4301, 10424
BarI GAAGNNNNNNTAC 8 cut(s) 5562, 5594, 8091, 8123, 8484, 8516, 9711, 9743
BauI CACGAG 1 cut(s) 4554
Bbv12I GWGCWC 7 cut(s) 383, 1967, 4301, 8871, 10424, 10459, 10812
BbvCI CCTCAGC 1 cut(s) 10997
BceAI ACGGC 5 cut(s) 848, 2276, 2611, 4576, 10384
BcgI CGANNNNNNTGC 6 cut(s) 4636, 4670, 4821, 4855, 8184, 8218
BciVI GTATCC 4 cut(s) 918, 1398, 6982, 10261
BclI TGATCA 6 cut(s) 1413, 3043, 3766, 5932, 8749, 10936
BcnI CCSGG 5 cut(s) 423, 810, 2199, 9658, 10838
BcuI ACTAGT 1 cut(s) 4349
BfoI RGCGCY 5 cut(s) 604, 617, 809, 2738, 10201
BfrI CTTAAG 3 cut(s) 221, 1157, 8020
BfuAI ACCTGC 3 cut(s) 3222, 6767, 10442
BfuI GTATCC 4 cut(s) 918, 1398, 6982, 10261
BglII AGATCT 7 cut(s) 1801, 6663, 8197, 8494, 9136, 9463, 10150
BlpI GCTNAGC 4 cut(s) 142, 597, 2706, 10792
BmcAI AGTACT 2 cut(s) 2143, 3641
Bme18I GGWCC 6 cut(s) 1754, 3179, 9487, 10040, 10088, 10896
BmeRI GACNNNNNGTC 1 cut(s) 7822
BmeT110I CYCGRG 3 cut(s) 6218, 9241, 9647
BmgBI CACGTC 2 cut(s) 8075, 8819
BmgT120I GGNCC 9 cut(s) 40, 1754, 2389, 3179, 6637, 9487, 10040, 10088, 10896
BmrI ACTGGG 1 cut(s) 4774
BmuI ACTGGG 1 cut(s) 4774
BoxI GACNNNNGTC 1 cut(s) 7509
BplI GAGNNNNNCTC 8 cut(s) 1204, 1236, 3341, 3373, 7152, 7184, 10799, 10831
Bpu10I CCTNAGC 2 cut(s) 1928, 10997
Bpu1102I GCTNAGC 4 cut(s) 142, 597, 2706, 10792
Bpu14I TTCGAA 2 cut(s) 1573, 9854
BpuMI CCSGG 5 cut(s) 423, 810, 2199, 9658, 10838
BsaAI YACGTR 1 cut(s) 9623
BsaHI GRCGYC 1 cut(s) 659
BsaI GGTCTC 8 cut(s) 1207, 1518, 2007, 4532, 4843, 9275, 9824, 10224
BsaWI WCCGGW 2 cut(s) 7213, 8971
BsaXI ACNNNNNCTCC 6 cut(s) 2761, 2791, 7937, 7967, 9537, 9567
Bse118I RCCGGY 1 cut(s) 296
Bse21I CCTNAGG 1 cut(s) 2385
BseRI GAGGAG 7 cut(s) 757, 899, 2800, 4945, 7328, 9168, 9737
BseSI GKGCMC 1 cut(s) 4326
BseYI CCCAGC 4 cut(s) 868, 1339, 4732, 7250
BsgI GTGCAG 6 cut(s) 1248, 1385, 1824, 6795, 7107, 10770
Bsh1236I CGCG 4 cut(s) 538, 5269, 5849, 9225
Bsh1285I CGRYCG 2 cut(s) 542, 1167
BshNI GGYRCC 4 cut(s) 3136, 5360, 8102, 8811
BsiEI CGRYCG 2 cut(s) 542, 1167
BsiHKAI GWGCWC 7 cut(s) 383, 1967, 4301, 8871, 10424, 10459, 10812
BsiHKCI CYCGRG 3 cut(s) 6218, 9241, 9647
BslFI GGGAC 8 cut(s) 2461, 4758, 5000, 5058, 8771, 8805, 9630, 10637
BsmBI CGTCTC 1 cut(s) 1224
BsmFI GGGAC 8 cut(s) 2461, 4758, 5000, 5058, 8771, 8805, 9630, 10637
BsmI GAATGC 5 cut(s) 3716, 4684, 5385, 5444, 9662
Bso31I GGTCTC 8 cut(s) 1207, 1518, 2007, 4532, 4843, 9275, 9824, 10224
BsoBI CYCGRG 3 cut(s) 6218, 9241, 9647
Bsp119I TTCGAA 2 cut(s) 1573, 9854
Bsp1407I TGTACA 3 cut(s) 2043, 2822, 3311
Bsp1720I GCTNAGC 4 cut(s) 142, 597, 2706, 10792
Bsp19I CCATGG 2 cut(s) 6875, 10310
BspFNI CGCG 4 cut(s) 538, 5269, 5849, 9225
BspHI TCATGA 6 cut(s) 232, 1374, 3442, 4567, 7495, 8077
BspMAI CTGCAG 7 cut(s) 696, 1039, 1962, 2415, 5499, 8197, 10117
BspMI ACCTGC 3 cut(s) 3222, 6767, 10442
BspQI GCTCTTC 1 cut(s) 5193
BspT104I TTCGAA 2 cut(s) 1573, 9854
BspT107I GGYRCC 4 cut(s) 3136, 5360, 8102, 8811
BspTI CTTAAG 3 cut(s) 221, 1157, 8020
BspTNI GGTCTC 8 cut(s) 1207, 1518, 2007, 4532, 4843, 9275, 9824, 10224
BsrBI CCGCTC 1 cut(s) 1407
BsrFI RCCGGY 1 cut(s) 296
BsrGI TGTACA 3 cut(s) 2043, 2822, 3311
BssAI RCCGGY 1 cut(s) 296
BssNAI GTATAC 2 cut(s) 4144, 10482
BssNI GRCGYC 1 cut(s) 659
BssSI CACGAG 1 cut(s) 4554
Bst1107I GTATAC 2 cut(s) 4144, 10482
Bst2BI CACGAG 1 cut(s) 4554
BstACI GRCGYC 1 cut(s) 659
BstAFI CTTAAG 3 cut(s) 221, 1157, 8020
BstAPI GCANNNNNTGC 3 cut(s) 5227, 6401, 9880
BstAUI TGTACA 3 cut(s) 2043, 2822, 3311
BstBAI YACGTR 1 cut(s) 9623
BstBI TTCGAA 2 cut(s) 1573, 9854
BstDSI CCRYGG 2 cut(s) 6875, 10310
BstEII GGTNACC 3 cut(s) 3539, 9652, 10318
BstENI CCTNNNNNAGG 2 cut(s) 5368, 10903
BstFNI CGCG 4 cut(s) 538, 5269, 5849, 9225
BstH2I RGCGCY 5 cut(s) 604, 617, 809, 2738, 10201
BstMCI CGRYCG 2 cut(s) 542, 1167
BstNSI RCATGY 9 cut(s) 1535, 3827, 5678, 5945, 6627, 8171, 9266, 9883, 10746
BstPAI GACNNNNGTC 1 cut(s) 7509
BstPI GGTNACC 3 cut(s) 3539, 9652, 10318
BstSLI GKGCMC 1 cut(s) 4326
BstUI CGCG 4 cut(s) 538, 5269, 5849, 9225
BstXI CCANNNNNNTGG 5 cut(s) 1894, 3086, 7528, 8376, 9903
BstZ17I GTATAC 2 cut(s) 4144, 10482
Bsu36I CCTNAGG 1 cut(s) 2385
BsuI GTATCC 4 cut(s) 918, 1398, 6982, 10261
BtgI CCRYGG 2 cut(s) 6875, 10310
BtrI CACGTC 2 cut(s) 8075, 8819
BtsI GCAGTG 4 cut(s) 1336, 1967, 5716, 6942
BveI ACCTGC 3 cut(s) 3222, 6767, 10442
CaiI CAGNNNCTG 3 cut(s) 4739, 5927, 6401
CciI TCATGA 6 cut(s) 232, 1374, 3442, 4567, 7495, 8077
Cfr10I RCCGGY 1 cut(s) 296
Cfr13I GGNCC 9 cut(s) 40, 1754, 2389, 3179, 6637, 9487, 10040, 10088, 10896
CseI GACGC 4 cut(s) 648, 5838, 5975, 9062
CspCI CAANNNNNGTGG 4 cut(s) 6607, 6642, 10496, 10531
DraI TTTAAA 3 cut(s) 1128, 1515, 6585
DraIII CACNNNGTG 1 cut(s) 2093
DriI GACNNNNNGTC 1 cut(s) 7822
EaeI YGGCCR 1 cut(s) 10143
Eam1105I GACNNNNNGTC 1 cut(s) 7822
EciI GGCGGA 2 cut(s) 1755, 1891
Ecl136II GAGCTC 2 cut(s) 4299, 10422
Eco147I AGGCCT 1 cut(s) 5374
Eco24I GRGCYC 4 cut(s) 1904, 3198, 4301, 10424
Eco31I GGTCTC 8 cut(s) 1207, 1518, 2007, 4532, 4843, 9275, 9824, 10224
Eco32I GATATC 2 cut(s) 2478, 4074
Eco47I GGWCC 6 cut(s) 1754, 3179, 9487, 10040, 10088, 10896
Eco47III AGCGCT 2 cut(s) 2736, 10199
Eco53kI GAGCTC 2 cut(s) 4299, 10422
Eco81I CCTNAGG 1 cut(s) 2385
Eco88I CYCGRG 3 cut(s) 6218, 9241, 9647
Eco91I GGTNACC 3 cut(s) 3539, 9652, 10318
EcoICRI GAGCTC 2 cut(s) 4299, 10422
EcoNI CCTNNNNNAGG 2 cut(s) 5368, 10903
EcoO109I RGGNCCY 2 cut(s) 2389, 10088
EcoO65I GGTNACC 3 cut(s) 3539, 9652, 10318
EcoRI GAATTC 4 cut(s) 4441, 6475, 7016, 7111
EcoRV GATATC 2 cut(s) 2478, 4074
EcoT22I ATGCAT 9 cut(s) 1273, 4308, 5003, 5130, 5943, 6015, 8619, 8737, 9239
EcoT38I GRGCYC 4 cut(s) 1904, 3198, 4301, 10424
Esp3I CGTCTC 1 cut(s) 1224
FaqI GGGAC 8 cut(s) 2461, 4758, 5000, 5058, 8771, 8805, 9630, 10637
FauI CCCGC 1 cut(s) 1202
FauNDI CATATG 3 cut(s) 5937, 8333, 10240
FbaI TGATCA 6 cut(s) 1413, 3043, 3766, 5932, 8749, 10936
FblI GTMKAC 5 cut(s) 4143, 4313, 4357, 10481, 10542
FriOI GRGCYC 4 cut(s) 1904, 3198, 4301, 10424
FspAI RTGCGCAY 1 cut(s) 5645
FspI TGCGCA 2 cut(s) 5645, 9261
GsaI CCCAGC 4 cut(s) 872, 1343, 4736, 7254
HaeII RGCGCY 5 cut(s) 604, 617, 809, 2738, 10201
HgaI GACGC 4 cut(s) 648, 5838, 5975, 9062
Hin1I GRCGYC 1 cut(s) 659
HindIII AAGCTT 7 cut(s) 1154, 1576, 2254, 4079, 6530, 8849, 9686
HpaI GTTAAC 2 cut(s) 7291, 10723
Hpy99I CGWCG 3 cut(s) 414, 8509, 9356
Hsp92I GRCGYC 1 cut(s) 659
KpnI GGTACC 2 cut(s) 8106, 8815
Ksp22I TGATCA 6 cut(s) 1413, 3043, 3766, 5932, 8749, 10936
KspAI GTTAAC 2 cut(s) 7291, 10723
LguI GCTCTTC 1 cut(s) 5193
MbiI CCGCTC 1 cut(s) 1407
MfeI CAATTG 5 cut(s) 3787, 4893, 5649, 7737, 8745
Mph1103I ATGCAT 9 cut(s) 1273, 4308, 5003, 5130, 5943, 6015, 8619, 8737, 9239
MroXI GAANNNNTTC 8 cut(s) 2218, 2312, 2315, 3716, 4608, 9726, 9772, 9942
MspA1I CMGCKG 2 cut(s) 864, 7250
MspCI CTTAAG 3 cut(s) 221, 1157, 8020
MunI CAATTG 5 cut(s) 3787, 4893, 5649, 7737, 8745
Mva1269I GAATGC 5 cut(s) 3716, 4684, 5385, 5444, 9662
MvnI CGCG 4 cut(s) 538, 5269, 5849, 9225
NciI CCSGG 5 cut(s) 423, 810, 2199, 9658, 10838
NcoI CCATGG 2 cut(s) 6875, 10310
NdeI CATATG 3 cut(s) 5937, 8333, 10240
NsbI TGCGCA 2 cut(s) 5645, 9261
NsiI ATGCAT 9 cut(s) 1273, 4308, 5003, 5130, 5943, 6015, 8619, 8737, 9239
NspI RCATGY 9 cut(s) 1535, 3827, 5678, 5945, 6627, 8171, 9266, 9883, 10746
NspV TTCGAA 2 cut(s) 1573, 9854
OliI CACNNNNGTG 1 cut(s) 10822
PaeI GCATGC 1 cut(s) 3827
PaeR7I CTCGAG 1 cut(s) 6218
PagI TCATGA 6 cut(s) 232, 1374, 3442, 4567, 7495, 8077
PaqCI CACCTGC 1 cut(s) 10442
PasI CCCWGGG 1 cut(s) 10324
PceI AGGCCT 1 cut(s) 5374
PciI ACATGT 2 cut(s) 1531, 10742
PciSI GCTCTTC 1 cut(s) 5193
PcsI WCGNNNNNNNCGW 4 cut(s) 656, 8815, 9628, 10714
PctI GAATGC 5 cut(s) 3716, 4684, 5385, 5444, 9662
PdmI GAANNNNTTC 8 cut(s) 2218, 2312, 2315, 3716, 4608, 9726, 9772, 9942
PflFI GACNNNGTC 3 cut(s) 7799, 10086, 10174
PflMI CCANNNNNTGG 3 cut(s) 1085, 5881, 6916
PfoI TCCNGGA 2 cut(s) 10225, 10836
Ppu21I YACGTR 1 cut(s) 9623
PpuMI RGGWCCY 1 cut(s) 10088
PscI ACATGT 2 cut(s) 1531, 10742
PshAI GACNNNNGTC 1 cut(s) 7509
PshBI ATTAAT 3 cut(s) 4038, 4184, 4623
PsiI TTATAA 1 cut(s) 314
Psp124BI GAGCTC 2 cut(s) 4301, 10424
Psp5II RGGWCCY 1 cut(s) 10088
PspEI GGTNACC 3 cut(s) 3539, 9652, 10318
PspFI CCCAGC 4 cut(s) 868, 1339, 4732, 7250
PspPI GGNCC 9 cut(s) 40, 1754, 2389, 3179, 6637, 9487, 10040, 10088, 10896
PspPPI RGGWCCY 1 cut(s) 10088
PspXI VCTCGAGB 1 cut(s) 6218
PsrI GAACNNNNNNTAC 2 cut(s) 10566, 10598
PstI CTGCAG 7 cut(s) 696, 1039, 1962, 2415, 5499, 8197, 10117
PstNI CAGNNNCTG 3 cut(s) 4739, 5927, 6401
PsyI GACNNNGTC 3 cut(s) 7799, 10086, 10174
SacI GAGCTC 2 cut(s) 4301, 10424
SapI GCTCTTC 1 cut(s) 5193
Sau96I GGNCC 9 cut(s) 40, 1754, 2389, 3179, 6637, 9487, 10040, 10088, 10896
ScaI AGTACT 2 cut(s) 2143, 3641
Sfr274I CTCGAG 1 cut(s) 6218
SfuI TTCGAA 2 cut(s) 1573, 9854
SinI GGWCC 6 cut(s) 1754, 3179, 9487, 10040, 10088, 10896
SlaI CTCGAG 1 cut(s) 6218
SpeI ACTAGT 1 cut(s) 4349
SphI GCATGC 1 cut(s) 3827
SseBI AGGCCT 1 cut(s) 5374
SspI AATATT 7 cut(s) 1006, 2841, 4036, 4492, 8299, 9256, 10499
SstI GAGCTC 2 cut(s) 4301, 10424
StuI AGGCCT 1 cut(s) 5374
TaqII GACCGA 4 cut(s) 1181, 4719, 7812, 10560
TatI WGTACW 8 cut(s) 2043, 2141, 2822, 3311, 3639, 5146, 7872, 8984
TauI GCSGC 6 cut(s) 614, 1135, 6740, 8744, 9438, 9874
TspGWI ACGGA 3 cut(s) 398, 5752, 9955
Tth111I GACNNNGTC 3 cut(s) 7799, 10086, 10174
Van91I CCANNNNNTGG 3 cut(s) 1085, 5881, 6916
Vha464I CTTAAG 3 cut(s) 221, 1157, 8020
VpaK11BI GGWCC 6 cut(s) 1754, 3179, 9487, 10040, 10088, 10896
VspI ATTAAT 3 cut(s) 4038, 4184, 4623
XagI CCTNNNNNAGG 2 cut(s) 5368, 10903
XbaI TCTAGA 3 cut(s) 5117, 5446, 7334
XceI RCATGY 9 cut(s) 1535, 3827, 5678, 5945, 6627, 8171, 9266, 9883, 10746
XcmI CCANNNNNNNNNTGG 3 cut(s) 7630, 10034, 10763
XhoI CTCGAG 1 cut(s) 6218
XmiI GTMKAC 5 cut(s) 4143, 4313, 4357, 10481, 10542
XmnI GAANNNNTTC 8 cut(s) 2218, 2312, 2315, 3716, 4608, 9726, 9772, 9942
ZrmI AGTACT 2 cut(s) 2143, 3641
Zsp2I ATGCAT 9 cut(s) 1273, 4308, 5003, 5130, 5943, 6015, 8619, 8737, 9239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.