Rroxscaffold_1G00048590

Mitochondrial protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013588
Physical Location & Seq
Forward (+)
68652212 .. 68654057
1846 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00048590.1

Sequence Viewer

Length: 528 bp
ATGGAAGCCGCAGGGCATTGTGGGAAGACAGGACCGATGAAGAAAGATGGTAAAGCTTCAAAGAACAACTTTGGGGCAACTAATCCCTCTGAAGCAAACCAAGAAGCTCCAGTTCAGAGCGTGGATAGTCCATTGGAGTCATGGATCTTTAGCACTGCAGCGTGCTATCATGTTACACAACAGCGGGAGATTCTGGAGAACTATACTACTGGCAATTTTGGCAAGGTCTACCTAGCTACTGGTGATTCCATGGATGTTGTGGGCAAGGGTGATGTTCGCATCAAACTGTCCAGTAATGCAACATGGATTCTACGTGATGTGAAACATATTCCGGGTCATGTGTGGAATTTAATTTCTGTGGGTCAGTTGGCTGGAAAAGGCTATGGGATGACATTCTCTAGTGACGGCTGGAAGGTTTCAACAGGAGATTTTGCTTTTACTGCCCCCTTGATCCAAATCAGCCCAACCGCATCACTTACCTATCTGCTTCACCCTAGCTTGACAGTGTCAGTGTGGCTTAATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve zf-CCHC

Protein Analysis

175

Amino Acids

18.88

Weight (kDa)

6.96

Isoelectric Point (pI)

25.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 48 - 128 7.9e-19 Pol polyprotein, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 228
AciI CCGC 3 cut(s) 9, 184, 468
AclWI GGATC 2 cut(s) 152, 445
AcsI RAATTY 1 cut(s) 346
AcuI CTGAAG 1 cut(s) 111
AgsI TTSAA 2 cut(s) 60, 420
AluBI AGCT 4 cut(s) 56, 107, 236, 498
AluI AGCT 4 cut(s) 56, 107, 236, 498
AlwI GGATC 2 cut(s) 152, 445
ApeKI GCWGC 1 cut(s) 158
ApoI RAATTY 1 cut(s) 346
AspS9I GGNCC 1 cut(s) 32
AsuC2I CCSGG 1 cut(s) 333
AsuHPI GGTGA 3 cut(s) 254, 281, 482
AvaII GGWCC 1 cut(s) 32
BbsI GAAGAC 1 cut(s) 32
BbvI GCAGC 1 cut(s) 170
BccI CCATC 1 cut(s) 41
BceAI ACGGC 1 cut(s) 421
BcnI CCSGG 1 cut(s) 333
BfaI CTAG 3 cut(s) 233, 399, 495
BfmI CTRYAG 1 cut(s) 156
BisI GCNGC 2 cut(s) 9, 159
BlsI GCNGC 2 cut(s) 10, 160
Bme1390I CCNGG 1 cut(s) 333
Bme18I GGWCC 1 cut(s) 32
BmgT120I GGNCC 1 cut(s) 32
BmrFI CCNGG 1 cut(s) 333
BmsI GCATC 2 cut(s) 288, 479
BpiI GAAGAC 1 cut(s) 32
BpmI CTGGAG 2 cut(s) 93, 215
BpuMI CCSGG 1 cut(s) 333
BsaAI YACGTR 1 cut(s) 314
BsaBI GATNNNNATC 1 cut(s) 455
BsaJI CCNNGG 1 cut(s) 249
Bse1I ACTGG 4 cut(s) 110, 214, 244, 291
Bse8I GATNNNNATC 1 cut(s) 455
BseDI CCNNGG 1 cut(s) 249
BseGI GGATG 2 cut(s) 259, 393
BseJI GATNNNNATC 1 cut(s) 455
BseNI ACTGG 4 cut(s) 110, 214, 244, 291
BseXI GCAGC 1 cut(s) 170
BsiSI CCGG 1 cut(s) 332
Bsp143I GATC 2 cut(s) 144, 450
Bsp19I CCATGG 1 cut(s) 249
BspACI CCGC 3 cut(s) 9, 184, 468
BspMAI CTGCAG 1 cut(s) 160
BspPI GGATC 2 cut(s) 152, 445
BsrI ACTGG 4 cut(s) 110, 214, 244, 291
BssECI CCNNGG 1 cut(s) 249
BssMI GATC 2 cut(s) 144, 450
BssT1I CCWWGG 1 cut(s) 249
Bst4CI ACNGT 2 cut(s) 288, 505
BstBAI YACGTR 1 cut(s) 314
BstC8I GCNNGC 1 cut(s) 163
BstDSI CCRYGG 1 cut(s) 249
BstF5I GGATG 2 cut(s) 259, 393
BstKTI GATC 2 cut(s) 147, 453
BstMBI GATC 2 cut(s) 144, 450
BstMWI GCNNNNNNNGC 2 cut(s) 219, 440
BstSCI CCNGG 1 cut(s) 331
BstSFI CTRYAG 1 cut(s) 156
BstV1I GCAGC 1 cut(s) 170
BstV2I GAAGAC 1 cut(s) 32
BstX2I RGATCY 1 cut(s) 144
BstYI RGATCY 1 cut(s) 144
BtgI CCRYGG 1 cut(s) 249
BtsCI GGATG 2 cut(s) 259, 393
BtsI GCAGTG 1 cut(s) 153
BtsIMutI CAGTG 3 cut(s) 153, 510, 516
Cac8I GCNNGC 1 cut(s) 163
Cfr13I GGNCC 1 cut(s) 32
CviAII CATG 5 cut(s) 141, 170, 250, 303, 338
DpnI GATC 2 cut(s) 146, 452
DpnII GATC 2 cut(s) 144, 450
Eco130I CCWWGG 1 cut(s) 249
Eco47I GGWCC 1 cut(s) 32
Eco57I CTGAAG 1 cut(s) 111
EcoT14I CCWWGG 1 cut(s) 249
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 5 cut(s) 144, 173, 253, 306, 341
FaiI YATR 8 cut(s) 142, 171, 204, 251, 304, 327, 339, 384
FalI AAGNNNNNCTT 2 cut(s) 53, 85
FatI CATG 5 cut(s) 140, 169, 249, 302, 337
FauI CCCGC 1 cut(s) 177
FblI GTMKAC 1 cut(s) 228
Fnu4HI GCNGC 2 cut(s) 9, 159
FokI GGATG 2 cut(s) 266, 400
Fsp4HI GCNGC 2 cut(s) 9, 159
FspBI CTAG 3 cut(s) 233, 399, 495
GluI GCNGC 2 cut(s) 9, 159
GsuI CTGGAG 2 cut(s) 93, 215
HapII CCGG 1 cut(s) 332
Hin1II CATG 5 cut(s) 144, 173, 253, 306, 341
HindIII AAGCTT 1 cut(s) 54
HinfI GANTC 4 cut(s) 137, 190, 245, 307
HpaII CCGG 1 cut(s) 332
HphI GGTGA 3 cut(s) 254, 281, 482
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 3 cut(s) 91, 117, 527
Hpy188III TCNNGA 1 cut(s) 194
Hpy8I GTNNAC 1 cut(s) 229
HpyAV CCTTC 1 cut(s) 406
HpyCH4III ACNGT 2 cut(s) 288, 505
HpyCH4IV ACGT 1 cut(s) 313
HpyCH4V TGCA 2 cut(s) 158, 299
HpyF10VI GCNNNNNNNGC 2 cut(s) 219, 440
HpySE526I ACGT 1 cut(s) 313
Hsp92II CATG 5 cut(s) 144, 173, 253, 306, 341
Kzo9I GATC 2 cut(s) 144, 450
LmnI GCTCC 1 cut(s) 112
Lsp1109I GCAGC 1 cut(s) 170
LweI GCATC 2 cut(s) 288, 479
MaeI CTAG 3 cut(s) 233, 399, 495
MaeII ACGT 1 cut(s) 313
MaeIII GTNAC 2 cut(s) 172, 401
MalI GATC 2 cut(s) 146, 452
MboI GATC 2 cut(s) 144, 450
MboII GAAGA 2 cut(s) 37, 52
MflI RGATCY 1 cut(s) 144
MluCI AATT 4 cut(s) 214, 346, 351, 520
MlyI GAGTC 1 cut(s) 146
MnlI CCTC 1 cut(s) 97
MseI TTAA 2 cut(s) 350, 519
MspA1I CMGCKG 1 cut(s) 184
MspI CCGG 1 cut(s) 332
MspR9I CCNGG 1 cut(s) 333
MwoI GCNNNNNNNGC 2 cut(s) 219, 440
NciI CCSGG 1 cut(s) 333
NcoI CCATGG 1 cut(s) 249
NdeII GATC 2 cut(s) 144, 450
NlaIII CATG 5 cut(s) 144, 173, 253, 306, 341
NmuCI GTSAC 1 cut(s) 401
PfeI GAWTC 3 cut(s) 190, 245, 307
PflFI GACNNNGTC 1 cut(s) 505
PkrI GCNGC 2 cut(s) 10, 160
PleI GAGTC 1 cut(s) 145
PpsI GAGTC 1 cut(s) 145
Ppu21I YACGTR 1 cut(s) 314
PspPI GGNCC 1 cut(s) 32
PstI CTGCAG 1 cut(s) 160
PsuI RGATCY 1 cut(s) 144
PsyI GACNNNGTC 1 cut(s) 505
SaqAI TTAA 2 cut(s) 350, 519
SatI GCNGC 2 cut(s) 9, 159
Sau3AI GATC 2 cut(s) 144, 450
Sau96I GGNCC 1 cut(s) 32
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 1 cut(s) 333
SetI ASST 9 cut(s) 58, 109, 228, 234, 238, 316, 417, 482, 500
SfaNI GCATC 2 cut(s) 288, 479
SfcI CTRYAG 1 cut(s) 156
SinI GGWCC 1 cut(s) 32
Sse9I AATT 4 cut(s) 214, 346, 351, 520
SsiI CCGC 3 cut(s) 9, 184, 468
SspMI CTAG 3 cut(s) 233, 399, 495
StyD4I CCNGG 1 cut(s) 331
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 2 cut(s) 288, 505
TaiI ACGT 1 cut(s) 316
TaqII GACCGA 1 cut(s) 49
TasI AATT 4 cut(s) 214, 346, 351, 520
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 3 cut(s) 190, 245, 307
Tru1I TTAA 2 cut(s) 350, 519
Tru9I TTAA 2 cut(s) 350, 519
TscAI CASTG 3 cut(s) 160, 510, 516
TseFI GTSAC 1 cut(s) 401
TseI GCWGC 1 cut(s) 158
Tsp45I GTSAC 1 cut(s) 401
TspDTI ATGAA 1 cut(s) 53
TspRI CASTG 3 cut(s) 160, 510, 516
Tth111I GACNNNGTC 1 cut(s) 505
VpaK11BI GGWCC 1 cut(s) 32
XapI RAATTY 1 cut(s) 346
XcmI CCANNNNNNNNNTGG 2 cut(s) 138, 256
XmiI GTMKAC 1 cut(s) 228
XspI CTAG 3 cut(s) 233, 399, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.