Rw5G020190

Mitochondrial protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0013588
Physical Location & Seq
Reverse (-)
26570457 .. 26572248
1792 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G020190.1

Sequence Viewer

Length: 1110 bp
ATGAGTGCAGAGGATATGGTTGACAAGTTTGATGGCACAAATTTCGATCTTTGGAGGACACAGATGATGAGCTACTTGCGTCTGAGGAGTCTTGATAAACCTTTGGAGGAGGACAAACCTCAAGACATGAGTCAGTTTGATTGGGACAAGCTTGACATGCAAGCTGTACTTGCTATTCGACGAACACTTGCCAGAAGCGTTAAAGACAAAGTCAAATCAAAGACAACAGCCGTCGGAATCATGAGCACTCTGTCGAAGATTTATGAGAAGCCACTTGCTGTGCACAAGTTAAACATGATGAAACGCCTGTTCAATCTAAAGATGGACGAACAATGTGGCTCTGTGAATCAGCATTTGAGCAATGTCAATGAACTTGTTGAGAAGTTGAGGTCGCTAGAAATCAACTTTGATGAAGACATTAAGGCTTTGTGTCTGTTATCTTCTCTGCCGGATAGCTGGGATGCAACTGTGACAAGTATTTGCTCTTCTTCAGGAAATACGAAGCTGGTTTACAGCGATGTCTGTGATACCATTTTAAGAGAAGAAATTAGAAGGAGGAACCAAGTCACAGAGCATTCAGAATCTGCTTTCCAAGCAAGGGCTAAATCTCGAGGTCGAAGCAAGTCTTGTTGCTGGCATTGTGGGAAGCCTGGACACAGGCAGAAGGACTGTGGAGCTTTGAACAATGTTGTTGAGGCAGTGAATGCAATTGATAGTGCATTGGAGTCATGGATCTTGACTCCTGGAGCAGCCCTTCATTGTTCACCACATCAAGAGATTATGCAAACGTATACTAGTGGGAATTTTGGCAAGGTCTACACAGCTTTTAATGAGTCCTTGGATGTTGTGGGTAAGGGTGATGTTCGTATCAAATTGCCCAATGGTGATATCTGGATCTTACCTGATGTGAGACACATACCGGGTTTGAAGCGAAATTTGATTTCTGTGGGTCAGTTGACTAATGAAGGCTATAGAGTATCTTGCTCTCCTGATGGCTGGATGGTTACAAGAGGAGCCTTTCTTTTAAATTGCTCTGCTGATGATTCTGATTCTACTCTAAAGGTTGTCTCTTGTTTTCCTCTCAATCCTAATCAAACTATACTTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve zf-CCHC

Protein Analysis

369

Amino Acids

41.38

Weight (kDa)

7.05

Isoelectric Point (pI)

42.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 34 - 188 5.2e-26 gag-polypeptide of LTR copia-type
Pol_BBD PF22936 244 - 324 3.6e-19 Pol polyprotein, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 791, 816
AclWI GGATC 2 cut(s) 740, 902
AcsI RAATTY 3 cut(s) 40, 802, 934
AcuI CTGAAG 1 cut(s) 474
AfaI GTAC 1 cut(s) 168
AfiI CCNNNNNNNGG 1 cut(s) 598
AgsI TTSAA 3 cut(s) 313, 682, 928
AhlI ACTAGT 1 cut(s) 794
AjnI CCWGG 2 cut(s) 649, 742
AluBI AGCT 7 cut(s) 72, 151, 164, 456, 505, 677, 824
AluI AGCT 7 cut(s) 72, 151, 164, 456, 505, 677, 824
Alw21I GWGCWC 2 cut(s) 248, 285
Alw26I GTCTC 2 cut(s) 904, 1072
Alw44I GTGCAC 1 cut(s) 281
AlwI GGATC 2 cut(s) 740, 902
AlwNI CAGNNNCTG 1 cut(s) 584
Ama87I CYCGRG 1 cut(s) 609
ApaLI GTGCAC 1 cut(s) 281
ApeKI GCWGC 1 cut(s) 749
ApoI RAATTY 3 cut(s) 40, 802, 934
AsuC2I CCSGG 1 cut(s) 921
AsuHPI GGTGA 3 cut(s) 756, 869, 896
AvaI CYCGRG 1 cut(s) 609
BaeGI GKGCMC 1 cut(s) 285
BarI GAAGNNNNNNTAC 4 cut(s) 469, 494, 501, 526
BbsI GAAGAC 1 cut(s) 420
Bbv12I GWGCWC 2 cut(s) 248, 285
BbvI GCAGC 1 cut(s) 761
BccI CCATC 4 cut(s) 26, 316, 986, 994
BceAI ACGGC 1 cut(s) 215
BcgI CGANNNNNNTGC 2 cut(s) 25, 59
BciT130I CCWGG 2 cut(s) 651, 744
BcnI CCSGG 1 cut(s) 921
BcoDI GTCTC 2 cut(s) 904, 1072
BcuI ACTAGT 1 cut(s) 794
BfaI CTAG 2 cut(s) 395, 795
BfmI CTRYAG 1 cut(s) 970
BisI GCNGC 1 cut(s) 750
BlsI GCNGC 1 cut(s) 751
Bme1390I CCNGG 3 cut(s) 651, 744, 921
BmeT110I CYCGRG 1 cut(s) 609
BmiI GGNNCC 2 cut(s) 560, 1015
BmrFI CCNGG 3 cut(s) 651, 744, 921
BmsI GCATC 1 cut(s) 451
BoxI GACNNNNGTC 1 cut(s) 129
BpiI GAAGAC 1 cut(s) 420
BpmI CTGGAG 1 cut(s) 765
BpuEI CTTGAG 1 cut(s) 105
BpuMI CCSGG 1 cut(s) 921
BsaJI CCNNGG 1 cut(s) 837
Bsc4I CCNNNNNNNGG 1 cut(s) 598
Bse3DI GCAATG 1 cut(s) 367
BseBI CCWGG 2 cut(s) 651, 744
BseDI CCNNGG 1 cut(s) 837
BseGI GGATG 3 cut(s) 466, 847, 1005
BseLI CCNNNNNNNGG 1 cut(s) 598
BseMI GCAATG 1 cut(s) 367
BseMII CTCAG 1 cut(s) 74
BseRI GAGGAG 3 cut(s) 100, 122, 1026
BseSI GKGCMC 1 cut(s) 285
BseXI GCAGC 1 cut(s) 761
BseYI CCCAGC 1 cut(s) 456
BsgI GTGCAG 1 cut(s) 27
BsiHKAI GWGCWC 2 cut(s) 248, 285
BsiHKCI CYCGRG 1 cut(s) 609
BsiSI CCGG 2 cut(s) 449, 920
BslFI GGGAC 1 cut(s) 158
BslI CCNNNNNNNGG 1 cut(s) 598
BsmAI GTCTC 2 cut(s) 904, 1072
BsmFI GGGAC 1 cut(s) 158
BsmI GAATGC 2 cut(s) 574, 709
BsoBI CYCGRG 1 cut(s) 609
Bsp1286I GDGCHC 2 cut(s) 248, 285
Bsp143I GATC 3 cut(s) 46, 732, 894
BspCNI CTCAG 1 cut(s) 75
BspHI TCATGA 1 cut(s) 240
BspLI GGNNCC 2 cut(s) 560, 1015
BspPI GGATC 2 cut(s) 740, 902
BspQI GCTCTTC 1 cut(s) 490
BsrDI GCAATG 1 cut(s) 367
BssECI CCNNGG 1 cut(s) 837
BssMI GATC 3 cut(s) 46, 732, 894
BssNAI GTATAC 1 cut(s) 792
BssT1I CCWWGG 1 cut(s) 837
Bst1107I GTATAC 1 cut(s) 792
Bst2UI CCWGG 2 cut(s) 651, 744
Bst4CI ACNGT 2 cut(s) 469, 671
Bst6I CTCTTC 1 cut(s) 490
BstAPI GCANNNNNTGC 1 cut(s) 704
BstC8I GCNNGC 2 cut(s) 162, 635
BstDEI CTNAG 1 cut(s) 83
BstF5I GGATG 3 cut(s) 466, 847, 1005
BstKTI GATC 3 cut(s) 49, 735, 897
BstMAI GTCTC 2 cut(s) 904, 1072
BstMBI GATC 3 cut(s) 46, 732, 894
BstMWI GCNNNNNNNGC 4 cut(s) 157, 170, 593, 704
BstNI CCWGG 2 cut(s) 651, 744
BstNSI RCATGY 1 cut(s) 160
BstPAI GACNNNNGTC 1 cut(s) 129
BstSCI CCNGG 3 cut(s) 649, 742, 919
BstSFI CTRYAG 1 cut(s) 970
BstSLI GKGCMC 1 cut(s) 285
BstV1I GCAGC 1 cut(s) 761
BstV2I GAAGAC 1 cut(s) 420
BstX2I RGATCY 2 cut(s) 732, 894
BstYI RGATCY 2 cut(s) 732, 894
BstZ17I GTATAC 1 cut(s) 792
BtgZI GCGATG 1 cut(s) 531
BtsCI GGATG 3 cut(s) 466, 847, 1005
BtsI GCAGTG 1 cut(s) 705
BtsIMutI CAGTG 1 cut(s) 705
Cac8I GCNNGC 2 cut(s) 162, 635
CaiI CAGNNNCTG 1 cut(s) 584
CciI TCATGA 1 cut(s) 240
CseI GACGC 1 cut(s) 68
Csp6I GTAC 1 cut(s) 167
CviAII CATG 5 cut(s) 127, 157, 241, 295, 729
CviQI GTAC 1 cut(s) 167
DdeI CTNAG 1 cut(s) 83
DpnI GATC 3 cut(s) 48, 734, 896
DpnII GATC 3 cut(s) 46, 732, 894
DraI TTTAAA 1 cut(s) 1026
Eam1104I CTCTTC 1 cut(s) 490
EarI CTCTTC 1 cut(s) 490
Eco130I CCWWGG 1 cut(s) 837
Eco32I GATATC 1 cut(s) 889
Eco57I CTGAAG 1 cut(s) 474
Eco88I CYCGRG 1 cut(s) 609
EcoRII CCWGG 2 cut(s) 649, 742
EcoRV GATATC 1 cut(s) 889
EcoT14I CCWWGG 1 cut(s) 837
ErhI CCWWGG 1 cut(s) 837
FaeI CATG 5 cut(s) 130, 160, 244, 298, 732
FalI AAGNNNNNCTT 4 cut(s) 153, 185, 610, 642
FaqI GGGAC 1 cut(s) 158
FatI CATG 5 cut(s) 126, 156, 240, 294, 728
FblI GTMKAC 2 cut(s) 791, 816
Fnu4HI GCNGC 1 cut(s) 750
FokI GGATG 3 cut(s) 473, 854, 1012
Fsp4HI GCNGC 1 cut(s) 750
FspBI CTAG 2 cut(s) 395, 795
GluI GCNGC 1 cut(s) 750
GsaI CCCAGC 1 cut(s) 460
GsuI CTGGAG 1 cut(s) 765
HapII CCGG 2 cut(s) 449, 920
HgaI GACGC 1 cut(s) 68
Hin1II CATG 5 cut(s) 130, 160, 244, 298, 732
HincII GTYRAC 2 cut(s) 22, 957
HindII GTYRAC 2 cut(s) 22, 957
HindIII AAGCTT 1 cut(s) 149
HpaII CCGG 2 cut(s) 449, 920
HphI GGTGA 3 cut(s) 756, 869, 896
Hpy166II GTNNAC 7 cut(s) 22, 283, 511, 764, 792, 817, 957
Hpy188I TCNGA 4 cut(s) 84, 236, 580, 1048
Hpy188III TCNNGA 9 cut(s) 92, 122, 241, 492, 609, 736, 773, 892, 989
Hpy8I GTNNAC 7 cut(s) 22, 283, 511, 764, 792, 817, 957
Hpy99I CGWCG 2 cut(s) 183, 236
HpyAV CCTTC 4 cut(s) 546, 658, 764, 959
HpyCH4III ACNGT 2 cut(s) 469, 671
HpyCH4IV ACGT 1 cut(s) 788
HpyCH4V TGCA 7 cut(s) 8, 160, 283, 464, 707, 719, 784
HpyF10VI GCNNNNNNNGC 4 cut(s) 157, 170, 593, 704
HpyF3I CTNAG 1 cut(s) 83
HpySE526I ACGT 1 cut(s) 788
Hsp92II CATG 5 cut(s) 130, 160, 244, 298, 732
Kzo9I GATC 3 cut(s) 46, 732, 894
LguI GCTCTTC 1 cut(s) 490
LmnI GCTCC 3 cut(s) 674, 746, 1013
Lsp1109I GCAGC 1 cut(s) 761
LweI GCATC 1 cut(s) 451
MaeI CTAG 2 cut(s) 395, 795
MaeII ACGT 1 cut(s) 788
MaeIII GTNAC 3 cut(s) 469, 565, 1003
MalI GATC 3 cut(s) 48, 734, 896
MboI GATC 3 cut(s) 46, 732, 894
MboII GAAGA 6 cut(s) 268, 425, 432, 477, 480, 554
MfeI CAATTG 1 cut(s) 708
MflI RGATCY 2 cut(s) 732, 894
MhlI GDGCHC 2 cut(s) 248, 285
MluCI AATT 7 cut(s) 40, 546, 708, 802, 872, 934, 1027
MlyI GAGTC 5 cut(s) 97, 139, 733, 734, 842
MmeI TCCRAC 1 cut(s) 214
MseI TTAA 6 cut(s) 201, 290, 420, 536, 828, 1025
MspI CCGG 2 cut(s) 449, 920
MspR9I CCNGG 3 cut(s) 651, 744, 921
MunI CAATTG 1 cut(s) 708
Mva1269I GAATGC 2 cut(s) 574, 709
MvaI CCWGG 2 cut(s) 651, 744
MwoI GCNNNNNNNGC 4 cut(s) 157, 170, 593, 704
NciI CCSGG 1 cut(s) 921
NdeII GATC 3 cut(s) 46, 732, 894
NlaIII CATG 5 cut(s) 130, 160, 244, 298, 732
NlaIV GGNNCC 2 cut(s) 560, 1015
NmuCI GTSAC 2 cut(s) 469, 565
NspI RCATGY 1 cut(s) 160
PaeR7I CTCGAG 1 cut(s) 609
PagI TCATGA 1 cut(s) 240
PciSI GCTCTTC 1 cut(s) 490
PctI GAATGC 2 cut(s) 574, 709
PfeI GAWTC 5 cut(s) 237, 346, 581, 1043, 1049
PflFI GACNNNGTC 1 cut(s) 209
PfoI TCCNGGA 1 cut(s) 742
PkrI GCNGC 1 cut(s) 751
PleI GAGTC 5 cut(s) 96, 138, 733, 733, 841
PpsI GAGTC 5 cut(s) 96, 138, 733, 733, 841
PshAI GACNNNNGTC 1 cut(s) 129
Psp6I CCWGG 2 cut(s) 649, 742
PspFI CCCAGC 1 cut(s) 456
PspGI CCWGG 2 cut(s) 649, 742
PspN4I GGNNCC 2 cut(s) 560, 1015
PstNI CAGNNNCTG 1 cut(s) 584
PsuI RGATCY 2 cut(s) 732, 894
PsyI GACNNNGTC 1 cut(s) 209
RsaI GTAC 1 cut(s) 168
RsaNI GTAC 1 cut(s) 167
SapI GCTCTTC 1 cut(s) 490
SaqAI TTAA 6 cut(s) 201, 290, 420, 536, 828, 1025
SatI GCNGC 1 cut(s) 750
Sau3AI GATC 3 cut(s) 46, 732, 894
SchI GAGTC 5 cut(s) 97, 139, 733, 734, 842
ScrFI CCNGG 3 cut(s) 651, 744, 921
SduI GDGCHC 2 cut(s) 248, 285
SfaNI GCATC 1 cut(s) 451
SfcI CTRYAG 1 cut(s) 970
Sfr274I CTCGAG 1 cut(s) 609
SlaI CTCGAG 1 cut(s) 609
SmlI CTYRAG 2 cut(s) 120, 609
SmoI CTYRAG 2 cut(s) 120, 609
SpeI ACTAGT 1 cut(s) 794
Sse9I AATT 7 cut(s) 40, 546, 708, 802, 872, 934, 1027
SspMI CTAG 2 cut(s) 395, 795
StyD4I CCNGG 3 cut(s) 649, 742, 919
StyI CCWWGG 1 cut(s) 837
TaaI ACNGT 2 cut(s) 469, 671
TaiI ACGT 1 cut(s) 791
TaqI TCGA 5 cut(s) 45, 178, 254, 610, 616
TasI AATT 7 cut(s) 40, 546, 708, 802, 872, 934, 1027
TatI WGTACW 1 cut(s) 166
TfiI GAWTC 5 cut(s) 237, 346, 581, 1043, 1049
Tru1I TTAA 6 cut(s) 201, 290, 420, 536, 828, 1025
Tru9I TTAA 6 cut(s) 201, 290, 420, 536, 828, 1025
TscAI CASTG 1 cut(s) 705
TseFI GTSAC 2 cut(s) 469, 565
TseI GCWGC 1 cut(s) 749
Tsp45I GTSAC 2 cut(s) 469, 565
TspDTI ATGAA 5 cut(s) 314, 384, 426, 746, 978
TspRI CASTG 1 cut(s) 705
Tth111I GACNNNGTC 1 cut(s) 209
VneI GTGCAC 1 cut(s) 281
XapI RAATTY 3 cut(s) 40, 802, 934
XceI RCATGY 1 cut(s) 160
XhoI CTCGAG 1 cut(s) 609
XmiI GTMKAC 2 cut(s) 791, 816
XspI CTAG 2 cut(s) 395, 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.