Rroxscaffold_1G00050680

helicase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005934
Physical Location & Seq
Forward (+)
71174250 .. 71177595
3346 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00050680.1

Sequence Viewer

Length: 504 bp
ATGGGCCTCGATACACACTTGGGCTTGGAGATCATAGAATTGGGTCCTAACATATGGTCTCCGACCATCACCGCTCAACCCATCCGCCATAGGAACCAACGATCTCTACAAACTATGCGCGCGCTTTACTCTATCGCCTATGATCGACGCTTCTGGATGTCGCCATCGATTGGACTTGAAGAAGTGATGGGTTTTCTTGAACGGTCGTTTCCCGATGTTGAAATAGTTGTTGAACTTGGGATAGTGCGCTCTTCCTTTTCTTTAGCAATTGAAAGTGGGCTTGACATTCGTAATGCAAACACCATTATAATTGAGGATGTTCAACAATTTGGCCTAGTACAGAATGACAATGTTGAGAATTCTATTGATAGCTACCACGAAGCCTTTGATGTTGTTGACCTCGGAAAATCAAGGAGACTAGGCCATGAGCTCTCCCTTGTTAGACTTGACTGCTATATGCAAATGTCATCCATGGAGAAGAGGAAATTGCAGTATCATGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CarD_CdnL_TRCF DEAD Helicase_C TRCF

Protein Analysis

167

Amino Acids

19.14

Weight (kDa)

5.19

Isoelectric Point (pI)

69.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 308
AccB7I CCANNNNNTGG 1 cut(s) 170
AccBSI CCGCTC 1 cut(s) 74
AccII CGCG 2 cut(s) 120, 122
AciI CCGC 2 cut(s) 72, 85
AcsI RAATTY 1 cut(s) 358
AfaI GTAC 1 cut(s) 339
AfiI CCNNNNNNNGG 1 cut(s) 170
AgsI TTSAA 6 cut(s) 179, 200, 221, 233, 272, 323
AluBI AGCT 2 cut(s) 372, 430
AluI AGCT 2 cut(s) 372, 430
Alw21I GWGCWC 1 cut(s) 432
Alw26I GTCTC 2 cut(s) 63, 409
AoxI GGCC 3 cut(s) 4, 331, 421
ApoI RAATTY 1 cut(s) 358
AspLEI GCGC 4 cut(s) 120, 122, 124, 249
AspS9I GGNCC 2 cut(s) 4, 44
AsuHPI GGTGA 1 cut(s) 61
AvaII GGWCC 1 cut(s) 44
BanII GRGCYC 1 cut(s) 432
Bbv12I GWGCWC 1 cut(s) 432
BccI CCATC 4 cut(s) 74, 89, 172, 181
BcoDI GTCTC 2 cut(s) 63, 409
BfaI CTAG 3 cut(s) 335, 419, 502
Bme18I GGWCC 1 cut(s) 44
BmgT120I GGNCC 2 cut(s) 4, 44
BmiI GGNNCC 2 cut(s) 45, 95
Bsa29I ATCGAT 1 cut(s) 167
BsaI GGTCTC 1 cut(s) 63
BsaJI CCNNGG 2 cut(s) 400, 471
Bsc4I CCNNNNNNNGG 1 cut(s) 170
BseCI ATCGAT 1 cut(s) 167
BseDI CCNNGG 2 cut(s) 400, 471
BseGI GGATG 4 cut(s) 81, 162, 322, 467
BseLI CCNNNNNNNGG 1 cut(s) 170
BsePI GCGCGC 2 cut(s) 118, 120
Bsh1236I CGCG 2 cut(s) 120, 122
Bsh1285I CGRYCG 1 cut(s) 206
BshFI GGCC 3 cut(s) 6, 333, 423
BshVI ATCGAT 1 cut(s) 167
BsiEI CGRYCG 1 cut(s) 206
BsiHKAI GWGCWC 1 cut(s) 432
BslI CCNNNNNNNGG 1 cut(s) 170
BsmAI GTCTC 2 cut(s) 63, 409
BsnI GGCC 3 cut(s) 6, 333, 423
Bso31I GGTCTC 1 cut(s) 63
Bsp1286I GDGCHC 1 cut(s) 432
Bsp143I GATC 3 cut(s) 30, 101, 142
Bsp19I CCATGG 1 cut(s) 471
BspACI CCGC 2 cut(s) 72, 85
BspANI GGCC 3 cut(s) 6, 333, 423
BspDI ATCGAT 1 cut(s) 167
BspFNI CGCG 2 cut(s) 120, 122
BspLI GGNNCC 2 cut(s) 45, 95
BspQI GCTCTTC 1 cut(s) 256
BspTNI GGTCTC 1 cut(s) 63
BsrBI CCGCTC 1 cut(s) 74
BssECI CCNNGG 2 cut(s) 400, 471
BssHII GCGCGC 2 cut(s) 118, 120
BssMI GATC 3 cut(s) 30, 101, 142
BssT1I CCWWGG 1 cut(s) 471
Bst4CI ACNGT 1 cut(s) 204
Bst6I CTCTTC 2 cut(s) 256, 473
BstC8I GCNNGC 2 cut(s) 120, 122
BstDSI CCRYGG 1 cut(s) 471
BstF5I GGATG 4 cut(s) 81, 162, 322, 467
BstFNI CGCG 2 cut(s) 120, 122
BstHHI GCGC 4 cut(s) 120, 122, 124, 249
BstKTI GATC 3 cut(s) 33, 104, 145
BstMAI GTCTC 2 cut(s) 63, 409
BstMBI GATC 3 cut(s) 30, 101, 142
BstMCI CGRYCG 1 cut(s) 206
BstUI CGCG 2 cut(s) 120, 122
Bsu15I ATCGAT 1 cut(s) 167
BsuRI GGCC 3 cut(s) 6, 333, 423
BsuTUI ATCGAT 1 cut(s) 167
BtgI CCRYGG 1 cut(s) 471
BtsCI GGATG 4 cut(s) 81, 162, 322, 467
Cac8I GCNNGC 2 cut(s) 120, 122
CfoI GCGC 4 cut(s) 120, 122, 124, 249
Cfr13I GGNCC 2 cut(s) 4, 44
ClaI ATCGAT 1 cut(s) 167
CseI GACGC 1 cut(s) 156
Csp6I GTAC 1 cut(s) 338
CviAII CATG 3 cut(s) 425, 472, 497
CviJI RGCY 8 cut(s) 6, 24, 280, 333, 372, 383, 423, 430
CviKI_1 RGCY 8 cut(s) 6, 24, 280, 333, 372, 383, 423, 430
CviQI GTAC 1 cut(s) 338
DpnI GATC 3 cut(s) 32, 103, 144
DpnII GATC 3 cut(s) 30, 101, 142
Eam1104I CTCTTC 2 cut(s) 256, 473
EarI CTCTTC 2 cut(s) 256, 473
EciI GGCGGA 1 cut(s) 74
Ecl136II GAGCTC 1 cut(s) 430
Eco130I CCWWGG 1 cut(s) 471
Eco24I GRGCYC 1 cut(s) 432
Eco31I GGTCTC 1 cut(s) 63
Eco47I GGWCC 1 cut(s) 44
Eco53kI GAGCTC 1 cut(s) 430
EcoICRI GAGCTC 1 cut(s) 430
EcoO109I RGGNCCY 1 cut(s) 44
EcoRI GAATTC 1 cut(s) 358
EcoT14I CCWWGG 1 cut(s) 471
EcoT38I GRGCYC 1 cut(s) 432
ErhI CCWWGG 1 cut(s) 471
FaeI CATG 3 cut(s) 428, 475, 500
FatI CATG 3 cut(s) 424, 471, 496
FauNDI CATATG 1 cut(s) 53
FokI GGATG 4 cut(s) 68, 169, 329, 454
FriOI GRGCYC 1 cut(s) 432
FspBI CTAG 3 cut(s) 335, 419, 502
GlaI GCGC 4 cut(s) 119, 121, 123, 248
HaeIII GGCC 3 cut(s) 6, 333, 423
HgaI GACGC 1 cut(s) 156
HhaI GCGC 4 cut(s) 120, 122, 124, 249
Hin1II CATG 3 cut(s) 428, 475, 500
Hin6I GCGC 4 cut(s) 118, 120, 122, 247
HinP1I GCGC 4 cut(s) 118, 120, 122, 247
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HphI GGTGA 1 cut(s) 61
Hpy166II GTNNAC 1 cut(s) 397
Hpy188I TCNGA 2 cut(s) 63, 404
Hpy188III TCNNGA 3 cut(s) 154, 197, 212
Hpy8I GTNNAC 1 cut(s) 397
Hpy99I CGWCG 1 cut(s) 150
HpyCH4III ACNGT 1 cut(s) 204
HpyCH4V TGCA 3 cut(s) 296, 460, 490
Hsp92II CATG 3 cut(s) 428, 475, 500
HspAI GCGC 4 cut(s) 118, 120, 122, 247
Kzo9I GATC 3 cut(s) 30, 101, 142
LguI GCTCTTC 1 cut(s) 256
LpnPI CCDG 1 cut(s) 139
MaeI CTAG 3 cut(s) 335, 419, 502
MalI GATC 3 cut(s) 32, 103, 144
MbiI CCGCTC 1 cut(s) 74
MboI GATC 3 cut(s) 30, 101, 142
MboII GAAGA 3 cut(s) 191, 243, 490
MfeI CAATTG 1 cut(s) 267
MhlI GDGCHC 1 cut(s) 432
MluCI AATT 6 cut(s) 38, 267, 309, 326, 358, 485
MmeI TCCRAC 1 cut(s) 86
MnlI CCTC 4 cut(s) 17, 307, 410, 474
MunI CAATTG 1 cut(s) 267
MvnI CGCG 2 cut(s) 120, 122
NcoI CCATGG 1 cut(s) 471
NdeI CATATG 1 cut(s) 53
NdeII GATC 3 cut(s) 30, 101, 142
NlaIII CATG 3 cut(s) 428, 475, 500
NlaIV GGNNCC 2 cut(s) 45, 95
PauI GCGCGC 2 cut(s) 118, 120
PciSI GCTCTTC 1 cut(s) 256
PflMI CCANNNNNTGG 1 cut(s) 170
PpuMI RGGWCCY 1 cut(s) 44
PsiI TTATAA 1 cut(s) 308
Psp124BI GAGCTC 1 cut(s) 432
Psp5II RGGWCCY 1 cut(s) 44
PspN4I GGNNCC 2 cut(s) 45, 95
PspPI GGNCC 2 cut(s) 4, 44
PspPPI RGGWCCY 1 cut(s) 44
PteI GCGCGC 2 cut(s) 118, 120
RsaI GTAC 1 cut(s) 339
RsaNI GTAC 1 cut(s) 338
SacI GAGCTC 1 cut(s) 432
SapI GCTCTTC 1 cut(s) 256
Sau3AI GATC 3 cut(s) 30, 101, 142
Sau96I GGNCC 2 cut(s) 4, 44
SduI GDGCHC 1 cut(s) 432
SetI ASST 3 cut(s) 374, 402, 432
SinI GGWCC 1 cut(s) 44
Sse9I AATT 6 cut(s) 38, 267, 309, 326, 358, 485
SsiI CCGC 2 cut(s) 72, 85
SspMI CTAG 3 cut(s) 335, 419, 502
SstI GAGCTC 1 cut(s) 432
StyI CCWWGG 1 cut(s) 471
TaaI ACNGT 1 cut(s) 204
TaqI TCGA 3 cut(s) 9, 145, 167
TasI AATT 6 cut(s) 38, 267, 309, 326, 358, 485
TatI WGTACW 1 cut(s) 337
Van91I CCANNNNNTGG 1 cut(s) 170
VpaK11BI GGWCC 1 cut(s) 44
XapI RAATTY 1 cut(s) 358
XspI CTAG 3 cut(s) 335, 419, 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.