Rh7BG302200

CarD-like/TRCF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0005934
Physical Location & Seq
Forward (+)
28694148 .. 28706393
12246 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG302200.1

Sequence Viewer

Length: 315 bp
ATGGAGAAATTTTCACAAGGTGAAATTACGATTCTCACATGCACTAATATAGTAGAAAGTGGGCTTGACATTCAGAATGCAAACACCATTATAATTGAGGATGTTCAACAGTTTGGCCTAGCACAGTTGTATCAGGCAAATGGTATGGCAGCATACTTTGTCACATTGACAGATTACTTGCTTGACAATTTCTTCTGGAGATCAGACATATATAAGAGAGATGTGAGAGCAGAAGAAGATGAACAGGTTGTAGGAGTTGGAATCCCTGGGATAAGAATTGGAATGAAAAGGTGTTGTACGTCCTTGGATCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CarD_CdnL_TRCF DEAD Helicase_C TRCF

Protein Analysis

104

Amino Acids

11.73

Weight (kDa)

4.32

Isoelectric Point (pI)

41.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 1 - 48 5.2e-09 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 92
AclWI GGATC 1 cut(s) 302
AcsI RAATTY 1 cut(s) 8
AdeI CACNNNGTG 1 cut(s) 20
AfaI GTAC 1 cut(s) 298
AgsI TTSAA 1 cut(s) 107
AjnI CCWGG 1 cut(s) 265
AlwI GGATC 1 cut(s) 302
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 1 cut(s) 149
ApoI RAATTY 1 cut(s) 8
AsuHPI GGTGA 1 cut(s) 32
BaeI ACNNNNGTAYC 2 cut(s) 113, 146
BamHI GGATCC 1 cut(s) 307
BbvI GCAGC 1 cut(s) 161
BciT130I CCWGG 1 cut(s) 267
BfaI CTAG 1 cut(s) 119
BisI GCNGC 1 cut(s) 150
BlsI GCNGC 1 cut(s) 151
Bme1390I CCNGG 1 cut(s) 267
BmiI GGNNCC 1 cut(s) 309
BmrFI CCNGG 1 cut(s) 267
BpmI CTGGAG 1 cut(s) 217
BsaJI CCNNGG 3 cut(s) 265, 266, 303
BseBI CCWGG 1 cut(s) 267
BseDI CCNNGG 3 cut(s) 265, 266, 303
BseGI GGATG 1 cut(s) 106
BseXI GCAGC 1 cut(s) 161
BshFI GGCC 1 cut(s) 117
BsmI GAATGC 1 cut(s) 82
BsnI GGCC 1 cut(s) 117
Bsp143I GATC 2 cut(s) 200, 307
BspANI GGCC 1 cut(s) 117
BspLI GGNNCC 1 cut(s) 309
BspPI GGATC 1 cut(s) 302
BssECI CCNNGG 3 cut(s) 265, 266, 303
BssMI GATC 2 cut(s) 200, 307
BssT1I CCWWGG 1 cut(s) 303
Bst2UI CCWGG 1 cut(s) 267
Bst4CI ACNGT 2 cut(s) 111, 126
BstDEI CTNAG 1 cut(s) 312
BstF5I GGATG 1 cut(s) 106
BstKTI GATC 2 cut(s) 203, 310
BstMBI GATC 2 cut(s) 200, 307
BstNI CCWGG 1 cut(s) 267
BstNSI RCATGY 1 cut(s) 42
BstSCI CCNGG 1 cut(s) 265
BstV1I GCAGC 1 cut(s) 161
BstX2I RGATCY 1 cut(s) 307
BstYI RGATCY 1 cut(s) 307
BsuRI GGCC 1 cut(s) 117
BtsCI GGATG 1 cut(s) 106
Csp6I GTAC 1 cut(s) 297
CviAII CATG 1 cut(s) 39
CviJI RGCY 2 cut(s) 64, 117
CviKI_1 RGCY 2 cut(s) 64, 117
CviQI GTAC 1 cut(s) 297
DdeI CTNAG 1 cut(s) 312
DpnI GATC 2 cut(s) 202, 309
DpnII GATC 2 cut(s) 200, 307
DraIII CACNNNGTG 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 303
EcoRII CCWGG 1 cut(s) 265
EcoT14I CCWWGG 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 303
FaeI CATG 1 cut(s) 42
FaiI YATR 8 cut(s) 40, 50, 92, 146, 154, 209, 211, 213
FatI CATG 1 cut(s) 38
Fnu4HI GCNGC 1 cut(s) 150
FokI GGATG 1 cut(s) 113
Fsp4HI GCNGC 1 cut(s) 150
FspBI CTAG 1 cut(s) 119
GluI GCNGC 1 cut(s) 150
GsuI CTGGAG 1 cut(s) 217
HaeIII GGCC 1 cut(s) 117
Hin1II CATG 1 cut(s) 42
HinfI GANTC 2 cut(s) 31, 261
HphI GGTGA 1 cut(s) 32
Hpy188I TCNGA 2 cut(s) 75, 205
Hpy188III TCNNGA 1 cut(s) 196
HpyCH4III ACNGT 2 cut(s) 111, 126
HpyCH4IV ACGT 1 cut(s) 299
HpyCH4V TGCA 2 cut(s) 42, 80
HpyF3I CTNAG 1 cut(s) 312
HpySE526I ACGT 1 cut(s) 299
Hsp92II CATG 1 cut(s) 42
Kzo9I GATC 2 cut(s) 200, 307
LpnPI CCDG 5 cut(s) 119, 181, 230, 252, 279
Lsp1109I GCAGC 1 cut(s) 161
MaeI CTAG 1 cut(s) 119
MaeII ACGT 1 cut(s) 299
MaeIII GTNAC 1 cut(s) 160
MalI GATC 2 cut(s) 202, 309
MboI GATC 2 cut(s) 200, 307
MboII GAAGA 3 cut(s) 184, 245, 248
MflI RGATCY 1 cut(s) 307
MluCI AATT 5 cut(s) 8, 24, 93, 187, 276
MmeI TCCRAC 1 cut(s) 238
MnlI CCTC 1 cut(s) 91
MspR9I CCNGG 1 cut(s) 267
Mva1269I GAATGC 1 cut(s) 82
MvaI CCWGG 1 cut(s) 267
NdeII GATC 2 cut(s) 200, 307
NlaIII CATG 1 cut(s) 42
NlaIV GGNNCC 1 cut(s) 309
NmuCI GTSAC 1 cut(s) 160
NspI RCATGY 1 cut(s) 42
PasI CCCWGGG 1 cut(s) 266
PctI GAATGC 1 cut(s) 82
PfeI GAWTC 2 cut(s) 31, 261
PkrI GCNGC 1 cut(s) 151
PsiI TTATAA 1 cut(s) 92
Psp6I CCWGG 1 cut(s) 265
PspGI CCWGG 1 cut(s) 265
PspN4I GGNNCC 1 cut(s) 309
PsuI RGATCY 1 cut(s) 307
RsaI GTAC 1 cut(s) 298
RsaNI GTAC 1 cut(s) 297
SatI GCNGC 1 cut(s) 150
Sau3AI GATC 2 cut(s) 200, 307
ScrFI CCNGG 1 cut(s) 267
SetI ASST 4 cut(s) 22, 249, 293, 302
Sse9I AATT 5 cut(s) 8, 24, 93, 187, 276
SspMI CTAG 1 cut(s) 119
StyD4I CCNGG 1 cut(s) 265
StyI CCWWGG 1 cut(s) 303
TaaI ACNGT 2 cut(s) 111, 126
TaiI ACGT 1 cut(s) 302
TasI AATT 5 cut(s) 8, 24, 93, 187, 276
TfiI GAWTC 2 cut(s) 31, 261
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 1 cut(s) 149
Tsp45I GTSAC 1 cut(s) 160
TspDTI ATGAA 2 cut(s) 255, 299
XapI RAATTY 1 cut(s) 8
XceI RCATGY 1 cut(s) 42
XspI CTAG 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.