Rroxscaffold_1G00072370

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006304
Physical Location & Seq
Reverse (-)
93451403 .. 93452394
992 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00072370.1

Sequence Viewer

Length: 981 bp
ATGTCTCTCATCTCCCGCCTCCGCCAATCCTTCCTCCTCCTCCACACGCGCCGCCTCTCCACCACCACCGAACCCTCCTCCAGAGACAAAATCCGCTCCCTCATCGCCCTCCTCAAAACCGAAAAAGACCCCCACAAAATCGTCTCCCTCTGCCAATCCGCCTCCCTCACCCCCGACTCCAACCTCCACCGCATCGCCCTCACACGCGCCGTCACCTCCCTCCGCGACTCCCCCACCCTAACCTCATCCCCCAATTCCTCGACCACTTCCTCCTCTCCCGCCCCGACCTCCAAACGCACCGTATCGCATCCCACGTCATCCTCCTCTACGGCCGGGCCCGCATGCCCGACCACGCCGTCCGCACCTTCGAGCTTTGCCATGAACTCGGACGTGAAGCTCCTCAACGCCCTGTTATTATCTTGCGTTTTCGCCGAGGATTACAAAGAGGTCACTCGGATTTACCGCGACTTTCCGAAGATTTATGGGATCGAGCCAAATCTGGACACTTACAATTGCGCACTCAAATCCCTTTCGGAATCCGGTTCCACCAGCTCTGCCTACTCTGTTTTGGCGGAGATGGAGAGGAGGAGGATCAAGCCCGATGGGAACACATTCGGACACTTGCTTTCGGGGTTTTACAGCGAGGAGAGGTTTGAGGATGTGGGAAATGTGATCAATTTGATGGAGAAGTATGGGTTCCAACCGGGGCTTGATACCTACAACGTTAGGATTCGGAGTCTTTGTAAGTTGAAGAAGTCGATGGAGGCCGAGGCATTGTTGGATGGGATGTTGGGGAGGGGTATAAAGCCGAATGCGGTCACGTTTTGCCATTTGATTCTCGGGTTTTGCAAGGAGGGGAATTTGGAGAAAGGGAAGAAGATGTTTGAGAAGATGGTGGAGAGGGGTTTGAGCCGGATTGCAACTGCTATTATACTCTGGTGTACTACTTGTGCACAGGAGGGGATTTCGAGAGTGCGTTGA

Protein Analysis

326

Amino Acids

35.92

Weight (kDa)

9.32

Isoelectric Point (pI)

52.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 165 - 212 5.4e-08 PPR repeat family
PPR_3 PF13812 188 - 242 5.2e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 204 - 249 5.3e-06 PPR repeat family
PPR_2 PF13041 238 - 284 5.4e-10 PPR repeat family
PPR_1 PF12854 266 - 299 3.5e-10 PPR repeat
PPR PF01535 273 - 303 4.7e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 355
Acc16I TGCGCA 1 cut(s) 517
AccBSI CCGCTC 1 cut(s) 96
AccII CGCG 4 cut(s) 49, 207, 225, 465
AclI AACGTT 1 cut(s) 723
AclWI GGATC 2 cut(s) 494, 599
AcoI YGGCCR 1 cut(s) 330
AcsI RAATTY 1 cut(s) 859
AfaI GTAC 1 cut(s) 943
AgsI TTSAA 1 cut(s) 751
AjiI CACGTC 2 cut(s) 315, 391
AluBI AGCT 3 cut(s) 372, 397, 552
AluI AGCT 3 cut(s) 372, 397, 552
Alw21I GWGCWC 1 cut(s) 955
Alw26I GTCTC 3 cut(s) 9, 78, 148
Alw44I GTGCAC 1 cut(s) 951
AlwI GGATC 2 cut(s) 494, 599
Ama87I CYCGRG 1 cut(s) 839
AoxI GGCC 3 cut(s) 330, 335, 765
ApaI GGGCCC 1 cut(s) 339
ApaLI GTGCAC 1 cut(s) 951
ApoI RAATTY 1 cut(s) 859
Asp700I GAANNNNTTC 1 cut(s) 611
AspLEI GCGC 3 cut(s) 51, 209, 518
AspS9I GGNCC 2 cut(s) 335, 336
AsuC2I CCSGG 2 cut(s) 334, 705
AsuHPI GGTGA 2 cut(s) 160, 205
AvaI CYCGRG 1 cut(s) 839
BaeGI GKGCMC 2 cut(s) 339, 955
BanII GRGCYC 1 cut(s) 339
Bbv12I GWGCWC 1 cut(s) 955
BccI CCATC 6 cut(s) 571, 596, 676, 754, 776, 886
BceAI ACGGC 3 cut(s) 194, 340, 345
BclI TGATCA 1 cut(s) 672
BcnI CCSGG 2 cut(s) 334, 705
BcoDI GTCTC 3 cut(s) 9, 78, 148
BisI GCNGC 1 cut(s) 52
BlsI GCNGC 1 cut(s) 53
Bme1390I CCNGG 2 cut(s) 334, 705
BmeT110I CYCGRG 1 cut(s) 839
BmgBI CACGTC 2 cut(s) 315, 391
BmgT120I GGNCC 2 cut(s) 335, 336
BmiI GGNNCC 3 cut(s) 337, 544, 698
BmrFI CCNGG 2 cut(s) 334, 705
BmsI GCATC 2 cut(s) 201, 316
BpmI CTGGAG 1 cut(s) 64
BpuMI CCSGG 2 cut(s) 334, 705
BsaJI CCNNGG 3 cut(s) 432, 704, 768
BsaWI WCCGGW 1 cut(s) 539
BsaXI ACNNNNNCTCC 2 cut(s) 727, 757
BseDI CCNNGG 3 cut(s) 432, 704, 768
BseGI GGATG 6 cut(s) 245, 307, 317, 664, 787, 792
BseSI GKGCMC 2 cut(s) 339, 955
BseX3I CGGCCG 1 cut(s) 330
Bsh1236I CGCG 4 cut(s) 49, 207, 225, 465
Bsh1285I CGRYCG 1 cut(s) 333
BshFI GGCC 3 cut(s) 332, 337, 767
BsiEI CGRYCG 1 cut(s) 333
BsiHKAI GWGCWC 1 cut(s) 955
BsiHKCI CYCGRG 1 cut(s) 839
BsiSI CCGG 4 cut(s) 333, 540, 704, 913
BsmAI GTCTC 3 cut(s) 9, 78, 148
BsmBI CGTCTC 1 cut(s) 148
BsmI GAATGC 1 cut(s) 817
BsnI GGCC 3 cut(s) 332, 337, 767
BsoBI CYCGRG 1 cut(s) 839
Bsp120I GGGCCC 1 cut(s) 335
Bsp1286I GDGCHC 2 cut(s) 339, 955
Bsp143I GATC 3 cut(s) 486, 591, 672
BspANI GGCC 3 cut(s) 332, 337, 767
BspFNI CGCG 4 cut(s) 49, 207, 225, 465
BspLI GGNNCC 3 cut(s) 337, 544, 698
BspPI GGATC 2 cut(s) 494, 599
BsrBI CCGCTC 1 cut(s) 96
BssECI CCNNGG 3 cut(s) 432, 704, 768
BssMI GATC 3 cut(s) 486, 591, 672
Bst4CI ACNGT 1 cut(s) 301
BstC8I GCNNGC 2 cut(s) 339, 343
BstF5I GGATG 6 cut(s) 245, 307, 317, 664, 787, 792
BstFNI CGCG 4 cut(s) 49, 207, 225, 465
BstHHI GCGC 3 cut(s) 51, 209, 518
BstKTI GATC 3 cut(s) 489, 594, 675
BstMAI GTCTC 3 cut(s) 9, 78, 148
BstMBI GATC 3 cut(s) 486, 591, 672
BstMCI CGRYCG 1 cut(s) 333
BstMWI GCNNNNNNNGC 1 cut(s) 338
BstNSI RCATGY 1 cut(s) 345
BstSCI CCNGG 2 cut(s) 332, 703
BstSLI GKGCMC 2 cut(s) 339, 955
BstUI CGCG 4 cut(s) 49, 207, 225, 465
BstZI CGGCCG 1 cut(s) 330
BsuRI GGCC 3 cut(s) 332, 337, 767
BtgZI GCGATG 2 cut(s) 88, 178
BtrI CACGTC 2 cut(s) 315, 391
BtsCI GGATG 6 cut(s) 245, 307, 317, 664, 787, 792
Cac8I GCNNGC 2 cut(s) 339, 343
CfoI GCGC 3 cut(s) 51, 209, 518
Cfr13I GGNCC 2 cut(s) 335, 336
Csp6I GTAC 1 cut(s) 942
CviAII CATG 2 cut(s) 342, 379
CviQI GTAC 1 cut(s) 942
DpnI GATC 3 cut(s) 488, 593, 674
DpnII GATC 3 cut(s) 486, 591, 672
DrdI GACNNNNNNGTC 1 cut(s) 355
DseDI GACNNNNNNGTC 1 cut(s) 355
EaeI YGGCCR 1 cut(s) 330
EagI CGGCCG 1 cut(s) 330
EciI GGCGGA 3 cut(s) 11, 148, 587
EclXI CGGCCG 1 cut(s) 330
Eco24I GRGCYC 1 cut(s) 339
Eco52I CGGCCG 1 cut(s) 330
Eco88I CYCGRG 1 cut(s) 839
EcoT38I GRGCYC 1 cut(s) 339
Esp3I CGTCTC 1 cut(s) 148
FaeI CATG 2 cut(s) 345, 382
FaiI YATR 6 cut(s) 343, 380, 483, 693, 803, 932
FatI CATG 2 cut(s) 341, 378
FauI CCCGC 3 cut(s) 23, 286, 346
FbaI TGATCA 1 cut(s) 672
Fnu4HI GCNGC 1 cut(s) 52
FokI GGATG 6 cut(s) 232, 294, 304, 671, 794, 799
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 1 cut(s) 52
FspI TGCGCA 1 cut(s) 517
GlaI GCGC 3 cut(s) 50, 208, 517
GluI GCNGC 1 cut(s) 52
GsuI CTGGAG 1 cut(s) 64
HaeIII GGCC 3 cut(s) 332, 337, 767
HapII CCGG 4 cut(s) 333, 540, 704, 913
HhaI GCGC 3 cut(s) 51, 209, 518
Hin1II CATG 2 cut(s) 345, 382
Hin6I GCGC 3 cut(s) 49, 207, 516
HinP1I GCGC 3 cut(s) 49, 207, 516
HinfI GANTC 6 cut(s) 176, 227, 536, 730, 736, 835
HpaII CCGG 4 cut(s) 333, 540, 704, 913
HphI GGTGA 2 cut(s) 160, 205
Hpy166II GTNNAC 2 cut(s) 942, 953
Hpy188I TCNGA 6 cut(s) 388, 456, 474, 535, 617, 735
Hpy188III TCNNGA 3 cut(s) 81, 500, 969
Hpy8I GTNNAC 2 cut(s) 942, 953
HpyAV CCTTC 2 cut(s) 40, 375
HpyCH4III ACNGT 1 cut(s) 301
HpyCH4IV ACGT 4 cut(s) 314, 390, 723, 821
HpyCH4V TGCA 3 cut(s) 849, 920, 953
HpyF10VI GCNNNNNNNGC 1 cut(s) 338
HpySE526I ACGT 4 cut(s) 314, 390, 723, 821
Hsp92II CATG 2 cut(s) 345, 382
HspAI GCGC 3 cut(s) 49, 207, 516
Ksp22I TGATCA 1 cut(s) 672
Kzo9I GATC 3 cut(s) 486, 591, 672
LmnI GCTCC 2 cut(s) 101, 402
LweI GCATC 2 cut(s) 201, 316
MaeII ACGT 4 cut(s) 314, 390, 723, 821
MaeIII GTNAC 3 cut(s) 211, 448, 817
MalI GATC 3 cut(s) 488, 593, 674
MbiI CCGCTC 1 cut(s) 96
MboI GATC 3 cut(s) 486, 591, 672
MboII GAAGA 5 cut(s) 487, 763, 886, 889, 901
MfeI CAATTG 1 cut(s) 511
MhlI GDGCHC 2 cut(s) 339, 955
MluCI AATT 4 cut(s) 253, 511, 676, 859
MlyI GAGTC 3 cut(s) 170, 221, 745
MmeI TCCRAC 3 cut(s) 204, 724, 759
MroXI GAANNNNTTC 1 cut(s) 611
MspI CCGG 4 cut(s) 333, 540, 704, 913
MspR9I CCNGG 2 cut(s) 334, 705
MunI CAATTG 1 cut(s) 511
Mva1269I GAATGC 1 cut(s) 817
MvnI CGCG 4 cut(s) 49, 207, 225, 465
MwoI GCNNNNNNNGC 1 cut(s) 338
NciI CCSGG 2 cut(s) 334, 705
NdeII GATC 3 cut(s) 486, 591, 672
NlaIII CATG 2 cut(s) 345, 382
NlaIV GGNNCC 3 cut(s) 337, 544, 698
NmeAIII GCCGAG 2 cut(s) 457, 793
NmuCI GTSAC 3 cut(s) 211, 448, 817
NsbI TGCGCA 1 cut(s) 517
NspI RCATGY 1 cut(s) 345
PaeI GCATGC 1 cut(s) 345
PcsI WCGNNNNNNNCGW 1 cut(s) 311
PctI GAATGC 1 cut(s) 817
PdmI GAANNNNTTC 1 cut(s) 611
PfeI GAWTC 3 cut(s) 536, 730, 835
PkrI GCNGC 1 cut(s) 53
PleI GAGTC 3 cut(s) 170, 221, 744
PpsI GAGTC 3 cut(s) 170, 221, 744
Psp1406I AACGTT 1 cut(s) 723
PspN4I GGNNCC 3 cut(s) 337, 544, 698
PspOMI GGGCCC 1 cut(s) 335
PspPI GGNCC 2 cut(s) 335, 336
RsaI GTAC 1 cut(s) 943
RsaNI GTAC 1 cut(s) 942
SatI GCNGC 1 cut(s) 52
Sau3AI GATC 3 cut(s) 486, 591, 672
Sau96I GGNCC 2 cut(s) 335, 336
SchI GAGTC 3 cut(s) 170, 221, 745
ScrFI CCNGG 2 cut(s) 334, 705
SduI GDGCHC 2 cut(s) 339, 955
SfaNI GCATC 2 cut(s) 201, 316
SphI GCATGC 1 cut(s) 345
Sse9I AATT 4 cut(s) 253, 511, 676, 859
StyD4I CCNGG 2 cut(s) 332, 703
TaaI ACNGT 1 cut(s) 301
TaiI ACGT 4 cut(s) 317, 393, 726, 824
TaqI TCGA 5 cut(s) 260, 368, 489, 758, 968
TasI AATT 4 cut(s) 253, 511, 676, 859
TatI WGTACW 1 cut(s) 941
TauI GCSGC 1 cut(s) 54
TfiI GAWTC 3 cut(s) 536, 730, 835
TseFI GTSAC 3 cut(s) 211, 448, 817
Tsp45I GTSAC 3 cut(s) 211, 448, 817
TspDTI ATGAA 1 cut(s) 395
VneI GTGCAC 1 cut(s) 951
XapI RAATTY 1 cut(s) 859
XceI RCATGY 1 cut(s) 345
XmnI GAANNNNTTC 1 cut(s) 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.