Rw5G002960

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0006304
Physical Location & Seq
Forward (+)
2788682 .. 2790061
1380 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G002960.1

Sequence Viewer

Length: 1323 bp
ATGGTAGCGCGTCTGACTCCAGATCAGAAGGTTGCGTGTTCGATTCACGTCGGGTTCAAATCCCTCCCATCCCGGAATATTGTTTTTCAGTCCTACTACCTTTTTTTTTTTGTAAAAAAACCAACTACCCCAAAATCTCAGTCCCTTTTCAGCTACCTCGCCACTGTCCCTCTCATAAACCCTACTAACCTCCACCTCAGCCCCTATGTCTCTCATCTCCCGCCTCCGCCAATCCTCCTCCTCTCCACACGCGCCGCCTCTCCACCACCACCGAACCCTCCTCCAAAGACAAAATCCGCTCCCTCATCGCCCTCCTCAAAACCGAAAAAGACCCCCACAAAATCGTCTCCCTCTGCCAATCCGCCTCCCTCACCCCCGACTCCAACCTCCACCGCATCGCCCTCACACGCGCCGTCACCTCCCTCCGCGACTCCCCCCACCCAAACCTCATCCCCCAATTCCTCGACCACTTCCTCCTCTCCCGCCCCGACCTCCAAACGCACCGCATCGCCTCCCACGTCATCCTCCTCTACGGCCGGGCCCGCATGCCCGACCACGCCGTCCGCACCTTCGAGCTCTGCCATGGACTCGGTATCCCCCAGGACGTCACTCGGATTTACCGCGACTTTCCGAAGATTTACGGGATCGACCAAATCTGGACACTTACAATTGCGCACTCAAATCCCTTTCGGAATCCGGTTCCACCGGCTCTGCCTACTCTGTTTGGCGGAGATGGAGAGGAGGAGGATCAAGCCCGATGGGAACACGTTCGGGCACTTGCTTTCGGGGTTTTACAGCGAGGAGAGGTTTGAGGATGTGGGAAATGTGATCAATTTGATGGACAAGTATGGGTTCCAACCGGGGCTTGATACCTACAACGTTAGGATTCGGAGTCTTTGTAAGTTGAAGAAGTCGATGGAGGCCGAGGCATTGTTGGATGGGATGTTGGGGAGGGGTATAAAGCCGAATGCGGTCACGTTTTGCCATTTGATTCTCGGGTTTTGCAAGGAGGGGAATTTGGAGAAAGGGAAGAAGATGTTTGAGAAGATGGTGGAGAGGGGGTTTGAGCCGGATTGCAACTGCTATTATACTCTGGTGTACTACTTGTGCACAGGAGGGGATTTCGAGAGTGCGTTGAGGTTTGCTAAGGAGAGCATTGAGAAGAATTGGGTGCCGAATTTTGCTACGATGAGGTTGCTTGTGGATGGACTTGTGAGCATTGGGAAGGTGGCTGAGGCTCGCCAGCTTATCGGCCAGATGAAGGAGAAGTTCACTGTGAATCAACATAGGTGGAGTGAAGTTGAAGAAGGCTTGCCACAGTGA

Protein Analysis

440

Amino Acids

48.0

Weight (kDa)

9.62

Isoelectric Point (pI)

61.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 242 - 294 1.6e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 290 - 336 8.3e-10 PPR repeat family
PPR_3 PF13812 311 - 364 4.6e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 318 - 351 5.2e-10 PPR repeat
PPR_2 PF13041 325 - 370 3.6e-09 PPR repeat family
PPR PF01535 325 - 355 5.4e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 559
AatII GACGTC 1 cut(s) 608
Acc16I TGCGCA 1 cut(s) 674
AccB1I GGYRCC 1 cut(s) 1171
AccBSI CCGCTC 1 cut(s) 299
AccII CGCG 5 cut(s) 10, 252, 410, 428, 623
AclI AACGTT 1 cut(s) 879
AclWI GGATC 2 cut(s) 652, 755
AcoI YGGCCR 2 cut(s) 534, 1252
AcsI RAATTY 2 cut(s) 1015, 1177
AcyI GRCGYC 1 cut(s) 605
AfaI GTAC 1 cut(s) 1100
AfiI CCNNNNNNNGG 1 cut(s) 1261
AflIII ACRYGT 1 cut(s) 765
AgsI TTSAA 3 cut(s) 58, 907, 1304
AjiI CACGTC 2 cut(s) 49, 519
AjnI CCWGG 1 cut(s) 599
AluBI AGCT 3 cut(s) 153, 576, 1246
AluI AGCT 3 cut(s) 153, 576, 1246
Alw21I GWGCWC 2 cut(s) 578, 1112
Alw26I GTCTC 2 cut(s) 214, 351
Alw44I GTGCAC 1 cut(s) 1108
AlwI GGATC 2 cut(s) 652, 755
Ama87I CYCGRG 1 cut(s) 995
AoxI GGCC 4 cut(s) 534, 539, 921, 1252
ApaI GGGCCC 1 cut(s) 543
ApaLI GTGCAC 1 cut(s) 1108
ApoI RAATTY 2 cut(s) 1015, 1177
ArsI GACNNNNNNTTYG 2 cut(s) 125, 157
Asp700I GAANNNNTTC 1 cut(s) 767
AspLEI GCGC 4 cut(s) 10, 254, 412, 675
AspS9I GGNCC 2 cut(s) 539, 540
AsuC2I CCSGG 3 cut(s) 73, 538, 861
AsuHPI GGTGA 2 cut(s) 363, 408
AvaI CYCGRG 1 cut(s) 995
BaeGI GKGCMC 3 cut(s) 543, 777, 1112
BanI GGYRCC 1 cut(s) 1171
BanII GRGCYC 2 cut(s) 543, 578
Bbv12I GWGCWC 2 cut(s) 578, 1112
BbvCI CCTCAGC 2 cut(s) 197, 1233
BccI CCATC 8 cut(s) 76, 727, 752, 832, 910, 932, 1042, 1199
BceAI ACGGC 3 cut(s) 397, 544, 549
BcgI CGANNNNNNTGC 2 cut(s) 1177, 1211
BciT130I CCWGG 1 cut(s) 601
BciVI GTATCC 1 cut(s) 604
BclI TGATCA 1 cut(s) 828
BcnI CCSGG 3 cut(s) 73, 538, 861
BcoDI GTCTC 2 cut(s) 214, 351
BfuI GTATCC 1 cut(s) 604
BisI GCNGC 1 cut(s) 255
BlsI GCNGC 1 cut(s) 256
Bme1390I CCNGG 4 cut(s) 73, 538, 601, 861
BmeT110I CYCGRG 1 cut(s) 995
BmgBI CACGTC 2 cut(s) 49, 519
BmgT120I GGNCC 2 cut(s) 539, 540
BmiI GGNNCC 4 cut(s) 541, 701, 854, 1173
BmrFI CCNGG 4 cut(s) 73, 538, 601, 861
BmsI GCATC 2 cut(s) 404, 515
Bpu10I CCTNAGC 3 cut(s) 197, 1146, 1233
BpuMI CCSGG 3 cut(s) 73, 538, 861
BsaHI GRCGYC 1 cut(s) 605
BsaJI CCNNGG 4 cut(s) 582, 599, 860, 924
BsaWI WCCGGW 1 cut(s) 696
BsaXI ACNNNNNCTCC 4 cut(s) 883, 913, 1046, 1076
Bsc4I CCNNNNNNNGG 1 cut(s) 1261
Bse118I RCCGGY 1 cut(s) 705
BseBI CCWGG 1 cut(s) 601
BseDI CCNNGG 4 cut(s) 582, 599, 860, 924
BseGI GGATG 7 cut(s) 68, 449, 521, 820, 943, 948, 1210
BseLI CCNNNNNNNGG 1 cut(s) 1261
BseMII CTCAG 3 cut(s) 152, 211, 1224
BseRI GAGGAG 9 cut(s) 227, 230, 270, 304, 466, 517, 754, 757, 815
BseSI GKGCMC 3 cut(s) 543, 777, 1112
BseX3I CGGCCG 1 cut(s) 534
Bsh1236I CGCG 5 cut(s) 10, 252, 410, 428, 623
Bsh1285I CGRYCG 1 cut(s) 537
BshFI GGCC 4 cut(s) 536, 541, 923, 1254
BshNI GGYRCC 1 cut(s) 1171
BsiEI CGRYCG 1 cut(s) 537
BsiHKAI GWGCWC 2 cut(s) 578, 1112
BsiHKCI CYCGRG 1 cut(s) 995
BsiSI CCGG 6 cut(s) 73, 537, 697, 706, 860, 1070
BslFI GGGAC 2 cut(s) 127, 152
BslI CCNNNNNNNGG 1 cut(s) 1261
BsmAI GTCTC 2 cut(s) 214, 351
BsmBI CGTCTC 1 cut(s) 351
BsmFI GGGAC 2 cut(s) 127, 152
BsmI GAATGC 1 cut(s) 973
BsnI GGCC 4 cut(s) 536, 541, 923, 1254
BsoBI CYCGRG 1 cut(s) 995
Bsp120I GGGCCC 1 cut(s) 539
Bsp1286I GDGCHC 4 cut(s) 543, 578, 777, 1112
Bsp143I GATC 4 cut(s) 22, 644, 747, 828
Bsp19I CCATGG 1 cut(s) 582
BspANI GGCC 4 cut(s) 536, 541, 923, 1254
BspCNI CTCAG 3 cut(s) 151, 210, 1225
BspFNI CGCG 5 cut(s) 10, 252, 410, 428, 623
BspLI GGNNCC 4 cut(s) 541, 701, 854, 1173
BspPI GGATC 2 cut(s) 652, 755
BspT107I GGYRCC 1 cut(s) 1171
BsrBI CCGCTC 1 cut(s) 299
BsrFI RCCGGY 1 cut(s) 705
BssAI RCCGGY 1 cut(s) 705
BssECI CCNNGG 4 cut(s) 582, 599, 860, 924
BssMI GATC 4 cut(s) 22, 644, 747, 828
BssNI GRCGYC 1 cut(s) 605
BssT1I CCWWGG 1 cut(s) 582
Bst2UI CCWGG 1 cut(s) 601
Bst4CI ACNGT 3 cut(s) 166, 1276, 1320
BstACI GRCGYC 1 cut(s) 605
BstC8I GCNNGC 5 cut(s) 543, 547, 1240, 1244, 1313
BstDEI CTNAG 4 cut(s) 138, 197, 1146, 1233
BstDSI CCRYGG 1 cut(s) 582
BstF5I GGATG 7 cut(s) 68, 449, 521, 820, 943, 948, 1210
BstFNI CGCG 5 cut(s) 10, 252, 410, 428, 623
BstHHI GCGC 4 cut(s) 10, 254, 412, 675
BstKTI GATC 4 cut(s) 25, 647, 750, 831
BstMAI GTCTC 2 cut(s) 214, 351
BstMBI GATC 4 cut(s) 22, 644, 747, 828
BstMCI CGRYCG 1 cut(s) 537
BstMWI GCNNNNNNNGC 1 cut(s) 542
BstNI CCWGG 1 cut(s) 601
BstNSI RCATGY 1 cut(s) 549
BstSCI CCNGG 4 cut(s) 71, 536, 599, 859
BstSLI GKGCMC 3 cut(s) 543, 777, 1112
BstUI CGCG 5 cut(s) 10, 252, 410, 428, 623
BstZI CGGCCG 1 cut(s) 534
BsuI GTATCC 1 cut(s) 604
BsuRI GGCC 4 cut(s) 536, 541, 923, 1254
BtgI CCRYGG 1 cut(s) 582
BtgZI GCGATG 3 cut(s) 291, 381, 492
BtrI CACGTC 2 cut(s) 49, 519
BtsCI GGATG 7 cut(s) 68, 449, 521, 820, 943, 948, 1210
BtsIMutI CAGTG 2 cut(s) 162, 1272
Cac8I GCNNGC 5 cut(s) 543, 547, 1240, 1244, 1313
CfoI GCGC 4 cut(s) 10, 254, 412, 675
Cfr10I RCCGGY 1 cut(s) 705
Cfr13I GGNCC 2 cut(s) 539, 540
Csp6I GTAC 1 cut(s) 1099
CspCI CAANNNNNGTGG 2 cut(s) 1271, 1306
CviAII CATG 2 cut(s) 546, 583
CviQI GTAC 1 cut(s) 1099
DdeI CTNAG 4 cut(s) 138, 197, 1146, 1233
DpnI GATC 4 cut(s) 24, 646, 749, 830
DpnII GATC 4 cut(s) 22, 644, 747, 828
DrdI GACNNNNNNGTC 1 cut(s) 559
DseDI GACNNNNNNGTC 1 cut(s) 559
EaeI YGGCCR 2 cut(s) 534, 1252
EagI CGGCCG 1 cut(s) 534
EciI GGCGGA 3 cut(s) 216, 351, 743
Ecl136II GAGCTC 1 cut(s) 576
EclXI CGGCCG 1 cut(s) 534
Eco130I CCWWGG 1 cut(s) 582
Eco24I GRGCYC 2 cut(s) 543, 578
Eco52I CGGCCG 1 cut(s) 534
Eco53kI GAGCTC 1 cut(s) 576
Eco88I CYCGRG 1 cut(s) 995
EcoICRI GAGCTC 1 cut(s) 576
EcoRII CCWGG 1 cut(s) 599
EcoT14I CCWWGG 1 cut(s) 582
EcoT38I GRGCYC 2 cut(s) 543, 578
ErhI CCWWGG 1 cut(s) 582
Esp3I CGTCTC 1 cut(s) 351
FaeI CATG 2 cut(s) 549, 586
FaiI YATR 8 cut(s) 176, 207, 547, 584, 849, 959, 1089, 1287
FaqI GGGAC 2 cut(s) 127, 152
FatI CATG 2 cut(s) 545, 582
FauI CCCGC 3 cut(s) 228, 490, 550
FbaI TGATCA 1 cut(s) 828
Fnu4HI GCNGC 1 cut(s) 255
FokI GGATG 7 cut(s) 55, 436, 508, 827, 950, 955, 1217
FriOI GRGCYC 2 cut(s) 543, 578
Fsp4HI GCNGC 1 cut(s) 255
FspI TGCGCA 1 cut(s) 674
GlaI GCGC 4 cut(s) 9, 253, 411, 674
GluI GCNGC 1 cut(s) 255
HaeIII GGCC 4 cut(s) 536, 541, 923, 1254
HapII CCGG 6 cut(s) 73, 537, 697, 706, 860, 1070
HhaI GCGC 4 cut(s) 10, 254, 412, 675
Hin1I GRCGYC 1 cut(s) 605
Hin1II CATG 2 cut(s) 549, 586
Hin6I GCGC 4 cut(s) 8, 252, 410, 673
HinP1I GCGC 4 cut(s) 8, 252, 410, 673
HpaII CCGG 6 cut(s) 73, 537, 697, 706, 860, 1070
HphI GGTGA 2 cut(s) 363, 408
Hpy166II GTNNAC 3 cut(s) 1099, 1110, 1272
Hpy188I TCNGA 6 cut(s) 15, 27, 614, 632, 692, 891
Hpy188III TCNNGA 3 cut(s) 20, 657, 1126
Hpy8I GTNNAC 3 cut(s) 1099, 1110, 1272
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 5 cut(s) 22, 579, 1219, 1255, 1301
HpyCH4III ACNGT 3 cut(s) 166, 1276, 1320
HpyCH4IV ACGT 6 cut(s) 48, 518, 605, 767, 879, 977
HpyCH4V TGCA 3 cut(s) 1005, 1077, 1110
HpyF10VI GCNNNNNNNGC 1 cut(s) 542
HpyF3I CTNAG 4 cut(s) 138, 197, 1146, 1233
HpySE526I ACGT 6 cut(s) 48, 518, 605, 767, 879, 977
Hsp92I GRCGYC 1 cut(s) 605
Hsp92II CATG 2 cut(s) 549, 586
HspAI GCGC 4 cut(s) 8, 252, 410, 673
Ksp22I TGATCA 1 cut(s) 828
Kzo9I GATC 4 cut(s) 22, 644, 747, 828
LmnI GCTCC 1 cut(s) 304
LweI GCATC 2 cut(s) 404, 515
MaeII ACGT 6 cut(s) 48, 518, 605, 767, 879, 977
MaeIII GTNAC 3 cut(s) 414, 606, 973
MalI GATC 4 cut(s) 24, 646, 749, 830
MbiI CCGCTC 1 cut(s) 299
MboI GATC 4 cut(s) 22, 644, 747, 828
MboII GAAGA 7 cut(s) 645, 919, 1042, 1045, 1057, 1174, 1316
MfeI CAATTG 1 cut(s) 668
MhlI GDGCHC 4 cut(s) 543, 578, 777, 1112
MluCI AATT 6 cut(s) 457, 668, 832, 1015, 1165, 1177
MlyI GAGTC 5 cut(s) 10, 373, 424, 581, 901
MmeI TCCRAC 3 cut(s) 407, 880, 915
MroXI GAANNNNTTC 1 cut(s) 767
MspI CCGG 6 cut(s) 73, 537, 697, 706, 860, 1070
MspR9I CCNGG 4 cut(s) 73, 538, 601, 861
MunI CAATTG 1 cut(s) 668
Mva1269I GAATGC 1 cut(s) 973
MvaI CCWGG 1 cut(s) 601
MvnI CGCG 5 cut(s) 10, 252, 410, 428, 623
MwoI GCNNNNNNNGC 1 cut(s) 542
NciI CCSGG 3 cut(s) 73, 538, 861
NcoI CCATGG 1 cut(s) 582
NdeII GATC 4 cut(s) 22, 644, 747, 828
NlaIII CATG 2 cut(s) 549, 586
NlaIV GGNNCC 4 cut(s) 541, 701, 854, 1173
NmeAIII GCCGAG 1 cut(s) 949
NmuCI GTSAC 3 cut(s) 414, 606, 973
NsbI TGCGCA 1 cut(s) 674
NspI RCATGY 1 cut(s) 549
PaeI GCATGC 1 cut(s) 549
PcsI WCGNNNNNNNCGW 1 cut(s) 515
PctI GAATGC 1 cut(s) 973
PdmI GAANNNNTTC 1 cut(s) 767
PfeI GAWTC 5 cut(s) 43, 693, 886, 991, 1279
PfoI TCCNGGA 1 cut(s) 71
PkrI GCNGC 1 cut(s) 256
PleI GAGTC 5 cut(s) 10, 373, 424, 581, 900
PpsI GAGTC 5 cut(s) 10, 373, 424, 581, 900
Psp124BI GAGCTC 1 cut(s) 578
Psp1406I AACGTT 1 cut(s) 879
Psp6I CCWGG 1 cut(s) 599
PspGI CCWGG 1 cut(s) 599
PspN4I GGNNCC 4 cut(s) 541, 701, 854, 1173
PspOMI GGGCCC 1 cut(s) 539
PspPI GGNCC 2 cut(s) 539, 540
RsaI GTAC 1 cut(s) 1100
RsaNI GTAC 1 cut(s) 1099
SacI GAGCTC 1 cut(s) 578
SatI GCNGC 1 cut(s) 255
Sau3AI GATC 4 cut(s) 22, 644, 747, 828
Sau96I GGNCC 2 cut(s) 539, 540
SchI GAGTC 5 cut(s) 10, 373, 424, 581, 901
ScrFI CCNGG 4 cut(s) 73, 538, 601, 861
SduI GDGCHC 4 cut(s) 543, 578, 777, 1112
SfaNI GCATC 2 cut(s) 404, 515
SphI GCATGC 1 cut(s) 549
Sse9I AATT 6 cut(s) 457, 668, 832, 1015, 1165, 1177
SspI AATATT 1 cut(s) 79
SstI GAGCTC 1 cut(s) 578
StyD4I CCNGG 4 cut(s) 71, 536, 599, 859
StyI CCWWGG 1 cut(s) 582
TaaI ACNGT 3 cut(s) 166, 1276, 1320
TaiI ACGT 6 cut(s) 51, 521, 608, 770, 882, 980
TaqI TCGA 6 cut(s) 41, 464, 572, 647, 914, 1125
TasI AATT 6 cut(s) 457, 668, 832, 1015, 1165, 1177
TatI WGTACW 1 cut(s) 1098
TauI GCSGC 1 cut(s) 257
TfiI GAWTC 5 cut(s) 43, 693, 886, 991, 1279
TscAI CASTG 2 cut(s) 169, 1279
TseFI GTSAC 3 cut(s) 414, 606, 973
Tsp45I GTSAC 3 cut(s) 414, 606, 973
TspDTI ATGAA 1 cut(s) 1274
TspRI CASTG 2 cut(s) 169, 1279
VneI GTGCAC 1 cut(s) 1108
XapI RAATTY 2 cut(s) 1015, 1177
XceI RCATGY 1 cut(s) 549
XmnI GAANNNNTTC 1 cut(s) 767
ZraI GACGTC 1 cut(s) 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.