Rroxscaffold_2G00088310

H ACA ribonucleoprotein complex non-core subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012463
Physical Location & Seq
Forward (+)
10281993 .. 10285126
3134 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00088310.1

Sequence Viewer

Length: 2163 bp
ATGGTAGGGTTCACCCCTGAAGTAGACCTCGATCAAGGTTCAAAGCTCAAGCTTTCAAAGGACCCACCTGACGCCGTTGATCCCACCAGACCCATCGACTTGTCCTTTGTAGATTCCTTGTTGGATTTCGACTCCATTGAGACTTGGTTCAGGGATTTTCCAAACCCAGATATGGCCGAGGCTGGGACGACGACTGTGGTGAAATCCGAGCCAATTGACTGTGAGCCTGGTTCTGTGGTGGAGGTGAAGTCTGAGGTAGGTGGTGGAGGCCAAATCGTTAATGGGTCTGACCCAATTGGTTGTGGGCCTGGTAGTGTGGTGAAGGTGAAGTCTGAAGTTTCAGAAAATTTGAGGAGTTCTATTGAGGAAGAGTTGGGGAAGGTTAGTTTGGTTGGAGGGAATGAAGAAAGTTTGGTTCTTGATAGTGGGAATGGAGTGAGTGAGGATTTGGAGACTCGAATCGAAGGTGTGGAGAGCAAAATGGAGAGTGGGAGGGAGAGTGAAAGTTCGGAGTCAGAGAGTGAGAGTTCATCAGCAGCATCGGCTTCGAGTAGTAGTTCCAGTGATGAGGAGGAGGATGAGAGTGAGAGTGATGACATGGATGAGGAGAAGGATGGAAGAGGGAATGTGAAGGTGGAGGCGGAGAAGGAGGGCAATGAGTTGGCTTGTGAACTTGAGGAAGGTGAGATAAGTGGTGAAGAGCAGAAAGGTAATGGAAGTGGGGATGAGGACGATGAGGAGGGCGCGGAGGTTGCGTGGAGTGATGTTGATATTTTCGATGAGGGAGATGAGGAAGAGGATGTTATAAAAGGTCCCATTCGTTCCAAAAATGAGCTCGAGGAACTTCCACCAGTTCCTCCAGTTAATGTGACCTTGGAACCACATCATCAAATGCTTCCTGTCGGAGTTGTTTTATCGGTTGTTGGTACCAAAGTCATTGTAGAAGGGGTTGAGAAGCACAACCCTCTGAACGAGGGTTCTATTCTGTGGATGACTGAAAGCAGGTCTCCGTTGGGACTGGTAGATGAAATTTTTGGACCTGTCATAAACCCTTACTATATGGTGAGATACAATACAGAAAGTGAAATCCCTGCCGGTATAGAAGTTGGAACACCAATCTCTTTTGTTCAGGAGTTTGCAGATCATGTGCTCAATAACAAAGATCTTTACAGAAAGGGTTATGATGCATCTGGTGCAAATGATGAAGAGGTGTCAGAAGCAGAATTTTCAGATGATGAAAAAGAAGCTGAATACAAGAGACTGCAAAAAATGACAAAGAGGGGAATGAATGACCAGAATGCAGGCAGTAAGAAAAACAACAGAAAAAGAGGTAAAAATAAGATGGGTGTCTGGAAAAATGGTCAATCTTCACCCCAGCAAACACCTCCCGATCAACACCACGAGCATTGCTCCTCAGCTGCACCTGCTCGTCGTTATGGTCCATCTTCTTCTGCAGTCTCAGAAGGTTTGGTTAGTGGGCGTGGATCAGTTCGACCATTTCCAGGTGCTACACAGGCCACTGGTATGAACGAAAATGGAGTTTGGTCAAATGGAATGCCGTGTCAGCCACAGCAAACACCCTTCCCTAATGCATTTCCAAATAATAATATGCCATTTTTTCCACAGTACCCTCATCAGATGTCTATGCCGGGCGGAATTCCATTCCATCAGCAGACAAATGTATTTTCTGGACCCATGGGTTCACAAGGGATGATGGGTCAACTTGGCTTCCACCAACCAGCATTTGGAGTAGGTTTCCAGGGACAACCTACACCAGGTCAACAGTTGAATTCCTTTCCAGGACCCATGAATGCATTTGGGATGAGTCAACATGGGTTCAACCAAAATGCATTTGGGATGGGTCAACAGGGATTCAACCCAAATGTTTTTGGGGTAGGTCAACATGGCTTCAACCCAAATGCGTTTGCGATGGTTCAACAGGGTCAGCATGGCTTCAACCAAAATACATTCGGGATAGGCTCACAGGGTCAACCTACAAACCTAAGCTTAGAACAGAATATGCCACAACCTGGGAACCCGGTTGTAGGTGATGCTGACGCTCCCCAACAATTTAGTCGAAGTACATCTGCTAATCGTGGAAGAAGACCATTTCGTCGAGGGGGTCGTCAGTTTGCAGGGGGAAGAGGAAGAGGTTCTAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000154 GO:0000454 GO:0000491 GO:0000493 GO:0001522 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005732 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010638 GO:0016043 GO:0016070 GO:0016072 GO:0016074 GO:0019219 GO:0019222 GO:0022607 GO:0022613 GO:0022618 GO:0031118 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032991 GO:0033043 GO:0033044 GO:0034470 GO:0034622 GO:0034641 GO:0034660 GO:0042254 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043487 GO:0043489 GO:0043933 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0045934 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051338 GO:0051347 GO:0051972 GO:0051973 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0065009 GO:0070034 GO:0071704 GO:0071826 GO:0071840 GO:0080090 GO:0090304 GO:0090669 GO:0097159 GO:1901360 GO:1901363 GO:1902369 GO:1904356 GO:1904358 GO:1905323 GO:1990904 GO:2000112 GO:2000278 GO:2000573 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gar1

Protein Analysis

720

Amino Acids

77.52

Weight (kDa)

4.53

Isoelectric Point (pI)

55.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gar1 PF04410 299 - 380 4.4e-17 Gar1/Naf1 RNA binding region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012463)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03530
fragaria_vesca FvH4_6g44910
malus_domestica MD09G1092000.v1.1 MD17G1081900.v1.1
prunus_persica Prupe.3G233000_v2.0.a1
pyrus_communis pycom09g01750 pycom17g08010
rosa_chinensis RchiOBHm_Chr2g0162091
rosa_laevigata RLG00000021322
rosa_multiflora Rmu_co8199028.1_g000001 Rmu_co8200688.1_g000001 Rmu_sc0004020.1_g000037
rosa_roxburghii Rroxscaffold_2G00088310
rosa_rugosa Rorug02G0494600
rosa_samantha Rh2AG560800 Rh2BG574100 Rh2CG544400 Rh2DG584100
rosa_wichuraiana Rw2G046450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 806
AarI CACCTGC 1 cut(s) 1432
AasI GACNNNNNNGTC 1 cut(s) 2112
Acc36I ACCTGC 2 cut(s) 993, 1432
Acc65I GGTACC 1 cut(s) 926
AccB1I GGYRCC 1 cut(s) 926
AccB7I CCANNNNNTGG 2 cut(s) 1745, 2030
AccI GTMKAC 1 cut(s) 24
AccII CGCG 1 cut(s) 746
AciI CCGC 3 cut(s) 641, 746, 1653
AclWI GGATC 2 cut(s) 74, 1492
AcoI YGGCCR 1 cut(s) 174
AcsI RAATTY 5 cut(s) 346, 1029, 1223, 1656, 1789
AcuI CTGAAG 2 cut(s) 39, 354
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 3 cut(s) 928, 1628, 2083
AfiI CCNNNNNNNGG 7 cut(s) 172, 183, 1502, 1745, 1775, 2030, 2045
AgsI TTSAA 8 cut(s) 42, 57, 1789, 1840, 1876, 1912, 1937, 1957
AjnI CCWGG 7 cut(s) 226, 307, 1501, 1758, 1774, 1798, 2029
AjuI GAANNNNNNNTTGG 2 cut(s) 371, 403
AluBI AGCT 6 cut(s) 46, 52, 835, 1247, 1418, 2007
AluI AGCT 6 cut(s) 46, 52, 835, 1247, 1418, 2007
Alw21I GWGCWC 2 cut(s) 837, 1152
Alw26I GTCTC 5 cut(s) 134, 446, 1011, 1252, 1462
AlwI GGATC 2 cut(s) 74, 1492
Ama87I CYCGRG 1 cut(s) 836
AoxI GGCC 4 cut(s) 174, 268, 305, 1515
ApeKI GCWGC 2 cut(s) 536, 1418
ApoI RAATTY 5 cut(s) 346, 1029, 1223, 1656, 1789
ArsI GACNNNNNNTTYG 2 cut(s) 89, 121
Asp700I GAANNNNTTC 1 cut(s) 2152
Asp718I GGTACC 1 cut(s) 926
AspLEI GCGC 1 cut(s) 746
AspS9I GGNCC 7 cut(s) 61, 305, 812, 1037, 1439, 1691, 1802
AsuC2I CCSGG 2 cut(s) 1650, 2039
AvaI CYCGRG 1 cut(s) 836
AvaII GGWCC 6 cut(s) 61, 812, 1037, 1439, 1691, 1802
BaeI ACNNNNGTAYC 2 cut(s) 918, 951
BanI GGYRCC 1 cut(s) 926
BanII GRGCYC 1 cut(s) 837
BauI CACGAG 1 cut(s) 1400
BbsI GAAGAC 1 cut(s) 2110
Bbv12I GWGCWC 2 cut(s) 837, 1152
BbvCI CCTCAGC 1 cut(s) 1414
BbvI GCAGC 2 cut(s) 548, 1405
BccI CCATC 8 cut(s) 101, 608, 1336, 1450, 1674, 1708, 1852, 1924
BceAI ACGGC 2 cut(s) 59, 1543
BcgI CGANNNNNNTGC 2 cut(s) 528, 562
BciT130I CCWGG 7 cut(s) 228, 309, 1503, 1760, 1776, 1800, 2031
BcnI CCSGG 2 cut(s) 1650, 2039
BcoDI GTCTC 5 cut(s) 134, 446, 1011, 1252, 1462
BfaI CTAG 1 cut(s) 2157
BfmI CTRYAG 1 cut(s) 1452
BfuAI ACCTGC 2 cut(s) 993, 1432
BglII AGATCT 1 cut(s) 1162
BisI GCNGC 2 cut(s) 537, 1419
BlsI GCNGC 2 cut(s) 538, 1420
Bme1390I CCNGG 9 cut(s) 228, 309, 1503, 1650, 1760, 1776, 1800, 2031, 2039
Bme18I GGWCC 6 cut(s) 61, 812, 1037, 1439, 1691, 1802
BmeT110I CYCGRG 1 cut(s) 836
BmgT120I GGNCC 7 cut(s) 61, 305, 812, 1037, 1439, 1691, 1802
BmiI GGNNCC 7 cut(s) 63, 814, 879, 928, 1693, 1804, 2036
BmrFI CCNGG 9 cut(s) 228, 309, 1503, 1650, 1760, 1776, 1800, 2031, 2039
BmsI GCATC 4 cut(s) 548, 1174, 1196, 2041
BpiI GAAGAC 1 cut(s) 2110
BplI GAGNNNNNCTC 2 cut(s) 1394, 1426
BpmI CTGGAG 1 cut(s) 843
Bpu10I CCTNAGC 2 cut(s) 1414, 2003
BpuEI CTTGAG 2 cut(s) 32, 695
BpuMI CCSGG 2 cut(s) 1650, 2039
BsaHI GRCGYC 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 1011
BsaJI CCNNGG 5 cut(s) 177, 873, 1695, 1759, 2030
BsaXI ACNNNNNCTCC 6 cut(s) 233, 263, 484, 514, 740, 770
Bsc4I CCNNNNNNNGG 7 cut(s) 172, 183, 1502, 1745, 1775, 2030, 2045
Bse118I RCCGGY 1 cut(s) 1094
Bse1I ACTGG 5 cut(s) 561, 851, 860, 1023, 1525
Bse3DI GCAATG 2 cut(s) 661, 1405
BseBI CCWGG 7 cut(s) 228, 309, 1503, 1760, 1776, 1800, 2031
BseDI CCNNGG 5 cut(s) 177, 873, 1695, 1759, 2030
BseGI GGATG 9 cut(s) 583, 607, 619, 730, 805, 996, 1716, 1827, 1863
BseLI CCNNNNNNNGG 7 cut(s) 172, 183, 1502, 1745, 1775, 2030, 2045
BseMI GCAATG 2 cut(s) 661, 1405
BseMII CTCAG 3 cut(s) 243, 1428, 1473
BseNI ACTGG 5 cut(s) 561, 851, 860, 1023, 1525
BseRI GAGGAG 6 cut(s) 367, 584, 587, 620, 752, 1402
BseXI GCAGC 2 cut(s) 548, 1405
BseYI CCCAGC 2 cut(s) 182, 1374
BsgI GTGCAG 1 cut(s) 1404
Bsh1236I CGCG 1 cut(s) 746
BshFI GGCC 4 cut(s) 176, 270, 307, 1517
BshNI GGYRCC 1 cut(s) 926
BsiHKAI GWGCWC 2 cut(s) 837, 1152
BsiHKCI CYCGRG 1 cut(s) 836
BsiSI CCGG 3 cut(s) 1095, 1649, 2039
BslFI GGGAC 4 cut(s) 199, 798, 1029, 1776
BslI CCNNNNNNNGG 7 cut(s) 172, 183, 1502, 1745, 1775, 2030, 2045
BsmAI GTCTC 5 cut(s) 134, 446, 1011, 1252, 1462
BsmFI GGGAC 4 cut(s) 199, 798, 1029, 1776
BsmI GAATGC 3 cut(s) 1303, 1560, 1816
BsnI GGCC 4 cut(s) 176, 270, 307, 1517
Bso31I GGTCTC 1 cut(s) 1011
BsoBI CYCGRG 1 cut(s) 836
Bsp1286I GDGCHC 2 cut(s) 837, 1152
Bsp143I GATC 6 cut(s) 31, 79, 1141, 1162, 1390, 1484
Bsp19I CCATGG 1 cut(s) 1695
BspACI CCGC 3 cut(s) 641, 746, 1653
BspANI GGCC 4 cut(s) 176, 270, 307, 1517
BspCNI CTCAG 3 cut(s) 244, 1427, 1472
BspFNI CGCG 1 cut(s) 746
BspLI GGNNCC 7 cut(s) 63, 814, 879, 928, 1693, 1804, 2036
BspMAI CTGCAG 1 cut(s) 1456
BspMI ACCTGC 2 cut(s) 993, 1432
BspPI GGATC 2 cut(s) 74, 1492
BspQI GCTCTTC 1 cut(s) 693
BspT107I GGYRCC 1 cut(s) 926
BspTNI GGTCTC 1 cut(s) 1011
BsrDI GCAATG 2 cut(s) 661, 1405
BsrFI RCCGGY 1 cut(s) 1094
BsrI ACTGG 5 cut(s) 561, 851, 860, 1023, 1525
BssAI RCCGGY 1 cut(s) 1094
BssECI CCNNGG 5 cut(s) 177, 873, 1695, 1759, 2030
BssMI GATC 6 cut(s) 31, 79, 1141, 1162, 1390, 1484
BssNI GRCGYC 1 cut(s) 72
BssSI CACGAG 1 cut(s) 1400
BssT1I CCWWGG 2 cut(s) 873, 1695
Bst2BI CACGAG 1 cut(s) 1400
Bst2UI CCWGG 7 cut(s) 228, 309, 1503, 1760, 1776, 1800, 2031
Bst4CI ACNGT 4 cut(s) 196, 221, 1626, 1785
Bst6I CTCTTC 7 cut(s) 363, 613, 693, 789, 1200, 2137, 2143
BstACI GRCGYC 1 cut(s) 72
BstAPI GCANNNNNTGC 1 cut(s) 1193
BstC8I GCNNGC 1 cut(s) 1303
BstDEI CTNAG 5 cut(s) 252, 1414, 1459, 2003, 2008
BstDSI CCRYGG 1 cut(s) 1695
BstENI CCTNNNNNAGG 1 cut(s) 1773
BstF5I GGATG 9 cut(s) 583, 607, 619, 730, 805, 996, 1716, 1827, 1863
BstFNI CGCG 1 cut(s) 746
BstHHI GCGC 1 cut(s) 746
BstKTI GATC 6 cut(s) 34, 82, 1144, 1165, 1393, 1487
BstMAI GTCTC 5 cut(s) 134, 446, 1011, 1252, 1462
BstMBI GATC 6 cut(s) 31, 79, 1141, 1162, 1390, 1484
BstMWI GCNNNNNNNGC 6 cut(s) 542, 752, 1193, 1424, 1514, 1564
BstNI CCWGG 7 cut(s) 228, 309, 1503, 1760, 1776, 1800, 2031
BstSCI CCNGG 9 cut(s) 226, 307, 1501, 1648, 1758, 1774, 1798, 2029, 2037
BstSFI CTRYAG 1 cut(s) 1452
BstUI CGCG 1 cut(s) 746
BstV1I GCAGC 2 cut(s) 548, 1405
BstV2I GAAGAC 1 cut(s) 2110
BstX2I RGATCY 1 cut(s) 1162
BstYI RGATCY 1 cut(s) 1162
BsuRI GGCC 4 cut(s) 176, 270, 307, 1517
BtgI CCRYGG 1 cut(s) 1695
BtgZI GCGATG 1 cut(s) 1943
BtsCI GGATG 9 cut(s) 583, 607, 619, 730, 805, 996, 1716, 1827, 1863
BtsIMutI CAGTG 2 cut(s) 568, 1518
BveI ACCTGC 2 cut(s) 993, 1432
Cac8I GCNNGC 1 cut(s) 1303
CfoI GCGC 1 cut(s) 746
Cfr10I RCCGGY 1 cut(s) 1094
Cfr13I GGNCC 7 cut(s) 61, 305, 812, 1037, 1439, 1691, 1802
CseI GACGC 2 cut(s) 80, 2066
CsiI ACCWGGT 1 cut(s) 1774
Csp6I GTAC 3 cut(s) 927, 1627, 2082
CspCI CAANNNNNGTGG 2 cut(s) 1388, 1423
CviAII CATG 7 cut(s) 598, 1145, 1696, 1807, 1832, 1904, 1949
CviQI GTAC 3 cut(s) 927, 1627, 2082
DdeI CTNAG 5 cut(s) 252, 1414, 1459, 2003, 2008
DpnI GATC 6 cut(s) 33, 81, 1143, 1164, 1392, 1486
DpnII GATC 6 cut(s) 31, 79, 1141, 1162, 1390, 1484
DrdI GACNNNNNNGTC 1 cut(s) 2112
DseDI GACNNNNNNGTC 1 cut(s) 2112
EaeI YGGCCR 1 cut(s) 174
Eam1104I CTCTTC 7 cut(s) 363, 613, 693, 789, 1200, 2137, 2143
EarI CTCTTC 7 cut(s) 363, 613, 693, 789, 1200, 2137, 2143
EciI GGCGGA 2 cut(s) 656, 1668
Ecl136II GAGCTC 1 cut(s) 835
Eco130I CCWWGG 2 cut(s) 873, 1695
Eco24I GRGCYC 1 cut(s) 837
Eco31I GGTCTC 1 cut(s) 1011
Eco47I GGWCC 6 cut(s) 61, 812, 1037, 1439, 1691, 1802
Eco53kI GAGCTC 1 cut(s) 835
Eco57I CTGAAG 2 cut(s) 39, 354
Eco88I CYCGRG 1 cut(s) 836
EcoICRI GAGCTC 1 cut(s) 835
EcoNI CCTNNNNNAGG 1 cut(s) 1773
EcoO109I RGGNCCY 3 cut(s) 61, 812, 1802
EcoRI GAATTC 2 cut(s) 1656, 1789
EcoRII CCWGG 7 cut(s) 226, 307, 1501, 1758, 1774, 1798, 2029
EcoT14I CCWWGG 2 cut(s) 873, 1695
EcoT22I ATGCAT 4 cut(s) 1189, 1594, 1816, 1852
EcoT38I GRGCYC 1 cut(s) 837
ErhI CCWWGG 2 cut(s) 873, 1695
FaeI CATG 7 cut(s) 601, 1148, 1699, 1810, 1835, 1907, 1952
FaqI GGGAC 4 cut(s) 199, 798, 1029, 1776
FatI CATG 7 cut(s) 597, 1144, 1695, 1806, 1831, 1903, 1948
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 2 cut(s) 537, 1419
FokI GGATG 9 cut(s) 590, 614, 626, 737, 812, 1003, 1723, 1834, 1870
FriOI GRGCYC 1 cut(s) 837
Fsp4HI GCNGC 2 cut(s) 537, 1419
FspBI CTAG 1 cut(s) 2157
GlaI GCGC 1 cut(s) 745
GluI GCNGC 2 cut(s) 537, 1419
GsaI CCCAGC 2 cut(s) 186, 1378
GsuI CTGGAG 1 cut(s) 843
HaeIII GGCC 4 cut(s) 176, 270, 307, 1517
HapII CCGG 3 cut(s) 1095, 1649, 2039
HgaI GACGC 2 cut(s) 80, 2066
HhaI GCGC 1 cut(s) 746
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 7 cut(s) 601, 1148, 1699, 1810, 1835, 1907, 1952
Hin6I GCGC 1 cut(s) 744
HinP1I GCGC 1 cut(s) 744
HincII GTYRAC 6 cut(s) 1721, 1781, 1829, 1865, 1901, 1991
HindII GTYRAC 6 cut(s) 1721, 1781, 1829, 1865, 1901, 1991
HindIII AAGCTT 2 cut(s) 50, 2005
HinfI GANTC 7 cut(s) 113, 131, 454, 459, 512, 1825, 1872
HpaII CCGG 3 cut(s) 1095, 1649, 2039
Hpy188III TCNNGA 6 cut(s) 419, 1130, 1351, 1388, 1689, 1972
Hpy99I CGWCG 3 cut(s) 193, 1434, 2118
HpyCH4III ACNGT 4 cut(s) 196, 221, 1626, 1785
HpyF10VI GCNNNNNNNGC 6 cut(s) 542, 752, 1193, 1424, 1514, 1564
HpyF3I CTNAG 5 cut(s) 252, 1414, 1459, 2003, 2008
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 7 cut(s) 601, 1148, 1699, 1810, 1835, 1907, 1952
HspAI GCGC 1 cut(s) 744
KpnI GGTACC 1 cut(s) 930
Kzo9I GATC 6 cut(s) 31, 79, 1141, 1162, 1390, 1484
LguI GCTCTTC 1 cut(s) 693
LmnI GCTCC 2 cut(s) 1415, 2065
Lsp1109I GCAGC 2 cut(s) 548, 1405
LweI GCATC 4 cut(s) 548, 1174, 1196, 2041
MabI ACCWGGT 1 cut(s) 1774
MaeI CTAG 1 cut(s) 2157
MaeIII GTNAC 1 cut(s) 868
MalI GATC 6 cut(s) 33, 81, 1143, 1164, 1392, 1486
MboI GATC 6 cut(s) 31, 79, 1141, 1162, 1390, 1484
MfeI CAATTG 2 cut(s) 213, 294
MflI RGATCY 1 cut(s) 1162
MhlI GDGCHC 2 cut(s) 837, 1152
MluCI AATT 8 cut(s) 213, 294, 346, 1029, 1223, 1656, 1789, 2069
MlyI GAGTC 4 cut(s) 125, 448, 521, 1834
MmeI TCCRAC 4 cut(s) 102, 373, 883, 1087
Mph1103I ATGCAT 4 cut(s) 1189, 1594, 1816, 1852
MroXI GAANNNNTTC 1 cut(s) 2152
MseI TTAA 2 cut(s) 279, 864
MslI CAYNNNNRTG 1 cut(s) 1523
MspA1I CMGCKG 1 cut(s) 1418
MspI CCGG 3 cut(s) 1095, 1649, 2039
MspR9I CCNGG 9 cut(s) 228, 309, 1503, 1650, 1760, 1776, 1800, 2031, 2039
MunI CAATTG 2 cut(s) 213, 294
Mva1269I GAATGC 3 cut(s) 1303, 1560, 1816
MvaI CCWGG 7 cut(s) 228, 309, 1503, 1760, 1776, 1800, 2031
MvnI CGCG 1 cut(s) 746
MwoI GCNNNNNNNGC 6 cut(s) 542, 752, 1193, 1424, 1514, 1564
NciI CCSGG 2 cut(s) 1650, 2039
NcoI CCATGG 1 cut(s) 1695
NdeII GATC 6 cut(s) 31, 79, 1141, 1162, 1390, 1484
NlaIII CATG 7 cut(s) 601, 1148, 1699, 1810, 1835, 1907, 1952
NlaIV GGNNCC 7 cut(s) 63, 814, 879, 928, 1693, 1804, 2036
NmeAIII GCCGAG 1 cut(s) 202
NmuCI GTSAC 1 cut(s) 868
NsiI ATGCAT 4 cut(s) 1189, 1594, 1816, 1852
PaeR7I CTCGAG 1 cut(s) 836
PaqCI CACCTGC 1 cut(s) 1432
PciSI GCTCTTC 1 cut(s) 693
PcsI WCGNNNNNNNCGW 1 cut(s) 2122
PctI GAATGC 3 cut(s) 1303, 1560, 1816
PdmI GAANNNNTTC 1 cut(s) 2152
PfeI GAWTC 3 cut(s) 113, 459, 1872
PflMI CCANNNNNTGG 2 cut(s) 1745, 2030
PfoI TCCNGGA 1 cut(s) 1798
PkrI GCNGC 2 cut(s) 538, 1420
PleI GAGTC 4 cut(s) 125, 448, 520, 1833
PpsI GAGTC 4 cut(s) 125, 448, 520, 1833
PpuMI RGGWCCY 3 cut(s) 61, 812, 1802
PsiI TTATAA 1 cut(s) 806
Psp124BI GAGCTC 1 cut(s) 837
Psp5II RGGWCCY 3 cut(s) 61, 812, 1802
Psp6I CCWGG 7 cut(s) 226, 307, 1501, 1758, 1774, 1798, 2029
PspFI CCCAGC 2 cut(s) 182, 1374
PspGI CCWGG 7 cut(s) 226, 307, 1501, 1758, 1774, 1798, 2029
PspN4I GGNNCC 7 cut(s) 63, 814, 879, 928, 1693, 1804, 2036
PspPI GGNCC 7 cut(s) 61, 305, 812, 1037, 1439, 1691, 1802
PspPPI RGGWCCY 3 cut(s) 61, 812, 1802
PspXI VCTCGAGB 1 cut(s) 836
PstI CTGCAG 1 cut(s) 1456
PsuI RGATCY 1 cut(s) 1162
PvuII CAGCTG 1 cut(s) 1418
RsaI GTAC 3 cut(s) 928, 1628, 2083
RsaNI GTAC 3 cut(s) 927, 1627, 2082
RseI CAYNNNNRTG 1 cut(s) 1523
SacI GAGCTC 1 cut(s) 837
SapI GCTCTTC 1 cut(s) 693
SaqAI TTAA 2 cut(s) 279, 864
SatI GCNGC 2 cut(s) 537, 1419
Sau3AI GATC 6 cut(s) 31, 79, 1141, 1162, 1390, 1484
Sau96I GGNCC 7 cut(s) 61, 305, 812, 1037, 1439, 1691, 1802
SchI GAGTC 4 cut(s) 125, 448, 521, 1834
ScrFI CCNGG 9 cut(s) 228, 309, 1503, 1650, 1760, 1776, 1800, 2031, 2039
SduI GDGCHC 2 cut(s) 837, 1152
SexAI ACCWGGT 1 cut(s) 1774
SfaNI GCATC 4 cut(s) 548, 1174, 1196, 2041
SfcI CTRYAG 1 cut(s) 1452
Sfr274I CTCGAG 1 cut(s) 836
SinI GGWCC 6 cut(s) 61, 812, 1037, 1439, 1691, 1802
SlaI CTCGAG 1 cut(s) 836
SmiMI CAYNNNNRTG 1 cut(s) 1523
SmlI CTYRAG 3 cut(s) 47, 674, 836
SmoI CTYRAG 3 cut(s) 47, 674, 836
Sse9I AATT 8 cut(s) 213, 294, 346, 1029, 1223, 1656, 1789, 2069
SsiI CCGC 3 cut(s) 641, 746, 1653
SspMI CTAG 1 cut(s) 2157
SstI GAGCTC 1 cut(s) 837
StyD4I CCNGG 9 cut(s) 226, 307, 1501, 1648, 1758, 1774, 1798, 2029, 2037
StyI CCWWGG 2 cut(s) 873, 1695
TaaI ACNGT 4 cut(s) 196, 221, 1626, 1785
TasI AATT 8 cut(s) 213, 294, 346, 1029, 1223, 1656, 1789, 2069
TatI WGTACW 1 cut(s) 2081
TfiI GAWTC 3 cut(s) 113, 459, 1872
Tru1I TTAA 2 cut(s) 279, 864
Tru9I TTAA 2 cut(s) 279, 864
TscAI CASTG 2 cut(s) 568, 1525
TseFI GTSAC 1 cut(s) 868
TseI GCWGC 2 cut(s) 536, 1418
Tsp45I GTSAC 1 cut(s) 868
TspDTI ATGAA 8 cut(s) 417, 519, 1041, 1218, 1251, 1301, 1541, 1823
TspGWI ACGGA 1 cut(s) 999
TspRI CASTG 2 cut(s) 568, 1525
Van91I CCANNNNNTGG 2 cut(s) 1745, 2030
VpaK11BI GGWCC 6 cut(s) 61, 812, 1037, 1439, 1691, 1802
XagI CCTNNNNNAGG 1 cut(s) 1773
XapI RAATTY 5 cut(s) 346, 1029, 1223, 1656, 1789
XcmI CCANNNNNNNNNTGG 3 cut(s) 278, 1742, 1850
XhoI CTCGAG 1 cut(s) 836
XmiI GTMKAC 1 cut(s) 24
XmnI GAANNNNTTC 1 cut(s) 2152
XspI CTAG 1 cut(s) 2157
Zsp2I ATGCAT 4 cut(s) 1189, 1594, 1816, 1852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.