Rroxscaffold_2G00106530

Initiation factor eIF2 gamma, C terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013757
Physical Location & Seq
Forward (+)
30116248 .. 30124170
7923 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_2G00106530.1

Sequence Viewer

Length: 390 bp
ATGCTTGATGTCAATGGGAGGTTGGCTGCTGTTGTTCCAATGCAGAAGTTTCGTGAGATTGATGACACAAAAATTCCTAGGAATGGTAGCATGATGAAACTGCAGAGAAATGTTTCTTTCGTAGATAGCCTGGCCCACAATGCGCTGATGGCAGCTATGCGCAATGGTGTCTCCATTATGGATGGAGCATTGCTTCTCGTAGCAGCTAAGGAAGAACTTCCCCCAGCACAAACCAAAGAGCATTTGGCTACTCTTGCAATTATGAGTCTTAAAGACATTATTTTCCTCCAAAAGAAAGTAGATCTTGAAGTTCAAGACGAGGATCGGGCCATTGAGCAACAACAACAAAAAATGGAGGAGTTCAACGAATCAGCCAACTGCTCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 eIF2_C

Protein Analysis

129

Amino Acids

14.43

Weight (kDa)

5.17

Isoelectric Point (pI)

39.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 33 - 119 2.4e-09 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 161
AclWI GGATC 1 cut(s) 330
AcsI RAATTY 1 cut(s) 72
AfiI CCNNNNNNNGG 1 cut(s) 83
AgsI TTSAA 3 cut(s) 308, 314, 364
AjnI CCWGG 1 cut(s) 129
AluBI AGCT 2 cut(s) 155, 206
AluI AGCT 2 cut(s) 155, 206
Alw26I GTCTC 1 cut(s) 175
AlwI GGATC 1 cut(s) 330
AoxI GGCC 2 cut(s) 132, 327
ApeKI GCWGC 3 cut(s) 26, 152, 203
ApoI RAATTY 1 cut(s) 72
Asp700I GAANNNNTTC 2 cut(s) 112, 216
AspA2I CCTAGG 1 cut(s) 77
AspLEI GCGC 2 cut(s) 145, 162
AspS9I GGNCC 2 cut(s) 133, 327
AvrII CCTAGG 1 cut(s) 77
BbvI GCAGC 3 cut(s) 13, 164, 215
BccI CCATC 2 cut(s) 142, 176
BcgI CGANNNNNNTGC 2 cut(s) 32, 66
BciT130I CCWGG 1 cut(s) 131
BcoDI GTCTC 1 cut(s) 175
BfaI CTAG 1 cut(s) 78
BfmI CTRYAG 1 cut(s) 101
BglII AGATCT 1 cut(s) 301
BisI GCNGC 3 cut(s) 27, 153, 204
BlnI CCTAGG 1 cut(s) 77
BlsI GCNGC 3 cut(s) 28, 154, 205
Bme1390I CCNGG 1 cut(s) 131
BmgT120I GGNCC 2 cut(s) 133, 327
BmrFI CCNGG 1 cut(s) 131
Bpu10I CCTNAGC 1 cut(s) 207
BsaJI CCNNGG 1 cut(s) 77
Bsc4I CCNNNNNNNGG 1 cut(s) 83
Bse3DI GCAATG 2 cut(s) 169, 188
BseBI CCWGG 1 cut(s) 131
BseDI CCNNGG 1 cut(s) 77
BseGI GGATG 1 cut(s) 187
BseLI CCNNNNNNNGG 1 cut(s) 83
BseMI GCAATG 2 cut(s) 169, 188
BseRI GAGGAG 1 cut(s) 371
BseXI GCAGC 3 cut(s) 13, 164, 215
BseYI CCCAGC 1 cut(s) 223
BshFI GGCC 2 cut(s) 134, 329
BslI CCNNNNNNNGG 1 cut(s) 83
BsmAI GTCTC 1 cut(s) 175
BsnI GGCC 2 cut(s) 134, 329
Bsp143I GATC 2 cut(s) 301, 322
BspANI GGCC 2 cut(s) 134, 329
BspMAI CTGCAG 1 cut(s) 105
BspPI GGATC 1 cut(s) 330
BsrDI GCAATG 2 cut(s) 169, 188
BssECI CCNNGG 1 cut(s) 77
BssMI GATC 2 cut(s) 301, 322
BssT1I CCWWGG 1 cut(s) 77
Bst2UI CCWGG 1 cut(s) 131
BstDEI CTNAG 1 cut(s) 207
BstF5I GGATG 1 cut(s) 187
BstHHI GCGC 2 cut(s) 145, 162
BstKTI GATC 2 cut(s) 304, 325
BstMAI GTCTC 1 cut(s) 175
BstMBI GATC 2 cut(s) 301, 322
BstMWI GCNNNNNNNGC 3 cut(s) 140, 149, 254
BstNI CCWGG 1 cut(s) 131
BstSCI CCNGG 1 cut(s) 129
BstSFI CTRYAG 1 cut(s) 101
BstV1I GCAGC 3 cut(s) 13, 164, 215
BstX2I RGATCY 1 cut(s) 301
BstYI RGATCY 1 cut(s) 301
BsuRI GGCC 2 cut(s) 134, 329
BtsCI GGATG 1 cut(s) 187
CfoI GCGC 2 cut(s) 145, 162
Cfr13I GGNCC 2 cut(s) 133, 327
CviAII CATG 1 cut(s) 91
CviJI RGCY 8 cut(s) 26, 129, 134, 155, 206, 248, 329, 374
CviKI_1 RGCY 8 cut(s) 26, 129, 134, 155, 206, 248, 329, 374
DdeI CTNAG 1 cut(s) 207
DpnI GATC 2 cut(s) 303, 324
DpnII GATC 2 cut(s) 301, 322
Eco130I CCWWGG 1 cut(s) 77
EcoRII CCWGG 1 cut(s) 129
EcoT14I CCWWGG 1 cut(s) 77
ErhI CCWWGG 1 cut(s) 77
FaeI CATG 1 cut(s) 94
FaiI YATR 4 cut(s) 92, 158, 179, 263
FalI AAGNNNNNCTT 2 cut(s) 288, 320
FatI CATG 1 cut(s) 90
Fnu4HI GCNGC 3 cut(s) 27, 153, 204
FokI GGATG 1 cut(s) 194
Fsp4HI GCNGC 3 cut(s) 27, 153, 204
FspBI CTAG 1 cut(s) 78
FspI TGCGCA 1 cut(s) 161
GlaI GCGC 2 cut(s) 144, 161
GluI GCNGC 3 cut(s) 27, 153, 204
GsaI CCCAGC 1 cut(s) 227
HaeIII GGCC 2 cut(s) 134, 329
HhaI GCGC 2 cut(s) 145, 162
Hin1II CATG 1 cut(s) 94
Hin6I GCGC 2 cut(s) 143, 160
HinP1I GCGC 2 cut(s) 143, 160
HinfI GANTC 2 cut(s) 265, 368
Hpy188III TCNNGA 3 cut(s) 53, 305, 314
HpyCH4V TGCA 3 cut(s) 43, 103, 257
HpyF10VI GCNNNNNNNGC 3 cut(s) 140, 149, 254
HpyF3I CTNAG 1 cut(s) 207
Hsp92II CATG 1 cut(s) 94
HspAI GCGC 2 cut(s) 143, 160
Kzo9I GATC 2 cut(s) 301, 322
LmnI GCTCC 1 cut(s) 185
LpnPI CCDG 3 cut(s) 116, 143, 237
Lsp1109I GCAGC 3 cut(s) 13, 164, 215
MaeI CTAG 1 cut(s) 78
MalI GATC 2 cut(s) 303, 324
MboI GATC 2 cut(s) 301, 322
MboII GAAGA 1 cut(s) 224
MflI RGATCY 1 cut(s) 301
MluCI AATT 2 cut(s) 72, 258
MlyI GAGTC 1 cut(s) 274
MnlI CCTC 4 cut(s) 12, 296, 313, 349
MroXI GAANNNNTTC 2 cut(s) 112, 216
MseI TTAA 1 cut(s) 270
MspR9I CCNGG 1 cut(s) 131
MvaI CCWGG 1 cut(s) 131
MwoI GCNNNNNNNGC 3 cut(s) 140, 149, 254
NdeII GATC 2 cut(s) 301, 322
NlaIII CATG 1 cut(s) 94
NsbI TGCGCA 1 cut(s) 161
PdmI GAANNNNTTC 2 cut(s) 112, 216
PfeI GAWTC 1 cut(s) 368
PkrI GCNGC 3 cut(s) 28, 154, 205
PleI GAGTC 1 cut(s) 273
PpsI GAGTC 1 cut(s) 273
Psp6I CCWGG 1 cut(s) 129
PspFI CCCAGC 1 cut(s) 223
PspGI CCWGG 1 cut(s) 129
PspPI GGNCC 2 cut(s) 133, 327
PstI CTGCAG 1 cut(s) 105
PsuI RGATCY 1 cut(s) 301
SaqAI TTAA 1 cut(s) 270
SatI GCNGC 3 cut(s) 27, 153, 204
Sau3AI GATC 2 cut(s) 301, 322
Sau96I GGNCC 2 cut(s) 133, 327
SchI GAGTC 1 cut(s) 274
ScrFI CCNGG 1 cut(s) 131
SetI ASST 3 cut(s) 23, 157, 208
SfcI CTRYAG 1 cut(s) 101
Sse9I AATT 2 cut(s) 72, 258
SspMI CTAG 1 cut(s) 78
StyD4I CCNGG 1 cut(s) 129
StyI CCWWGG 1 cut(s) 77
TasI AATT 2 cut(s) 72, 258
TfiI GAWTC 1 cut(s) 368
Tru1I TTAA 1 cut(s) 270
Tru9I TTAA 1 cut(s) 270
TseI GCWGC 3 cut(s) 26, 152, 203
TspDTI ATGAA 1 cut(s) 110
XapI RAATTY 1 cut(s) 72
XcmI CCANNNNNNNNNTGG 1 cut(s) 241
XmaJI CCTAGG 1 cut(s) 77
XmnI GAANNNNTTC 2 cut(s) 112, 216
XspI CTAG 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.