Rroxscaffold_3G00219960

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0015769
Physical Location & Seq
Forward (+)
2129774 .. 2132483
2710 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00219960.1

Sequence Viewer

Length: 609 bp
ATGCTCGATTTGGAAGCCATCCCAAGTTGGAACTCCGTCGTTGCTTCCGGGGAAACAGTGGAACCCCCACCGGTGGTGGTATCGGTCTCACCTCCCAAACTATCGGGGAGTCGCCGTGAAGCCCGATCCGAGTTGGCTAGGGTGGGTAAGAAGGCTAAGTTGGGGAAGGGAGTTGCTTCGAGCCCATTGACCTTTGAATCGGGCTTCGATCTTCTAAATACTAATGGGGTGATCTATTTATCCCCAAAGAAGCGGGGCCAGCCCAAAGGCAGCAGAAATGTAGCAAAGCCTAGGCTAGGGATGCCAAAGCCTAAAAAGAAGAGGTTTCCTATGAAGAATGGGAAACCAAAGAAGATGGAGGTGGTGGTGAGTTCATCTTCCTCGGAGGCGGTGCATGAGGAGGCCATAAGGCACAAGGTAGGAGAAACGAGGGGATGGTGCCACCATTCGGCGATGGCATCAGAGCCAATAAGGTTTGAGAAAAGTGGTAAATGGTATGCATCACAATGTGGTGGGAGCATGCCACATGTTGACATATTAATGGTTGCAACAACCTCAATGGTGTACATAACGTTAGGGTTGTGGATGGAAAAGATGCCTCATGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

202

Amino Acids

22.02

Weight (kDa)

10.01

Isoelectric Point (pI)

56.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 438
AciI CCGC 2 cut(s) 253, 389
AclI AACGTT 1 cut(s) 572
AclWI GGATC 1 cut(s) 120
AdeI CACNNNGTG 1 cut(s) 509
AfaI GTAC 1 cut(s) 566
AfiI CCNNNNNNNGG 3 cut(s) 73, 296, 448
AflIII ACRYGT 1 cut(s) 526
AgeI ACCGGT 1 cut(s) 70
AgsI TTSAA 1 cut(s) 197
AjuI GAANNNNNNNTTGG 2 cut(s) 143, 175
Alw26I GTCTC 1 cut(s) 91
AlwI GGATC 1 cut(s) 120
AoxI GGCC 2 cut(s) 256, 402
ApeKI GCWGC 1 cut(s) 270
AseI ATTAAT 1 cut(s) 539
AsiGI ACCGGT 1 cut(s) 70
AspA2I CCTAGG 1 cut(s) 290
AspS9I GGNCC 1 cut(s) 256
AsuC2I CCSGG 1 cut(s) 49
AsuHPI GGTGA 3 cut(s) 81, 241, 379
AvrII CCTAGG 1 cut(s) 290
BanI GGYRCC 1 cut(s) 438
BanII GRGCYC 1 cut(s) 185
BbvI GCAGC 1 cut(s) 282
BccI CCATC 5 cut(s) 26, 349, 429, 448, 580
BceAI ACGGC 1 cut(s) 99
BcnI CCSGG 1 cut(s) 49
BcoDI GTCTC 1 cut(s) 91
BfaI CTAG 4 cut(s) 138, 291, 296, 607
BisI GCNGC 1 cut(s) 271
BlnI CCTAGG 1 cut(s) 290
BlsI GCNGC 1 cut(s) 272
Bme1390I CCNGG 1 cut(s) 49
BmgT120I GGNCC 1 cut(s) 256
BmiI GGNNCC 3 cut(s) 63, 257, 440
BmrFI CCNGG 1 cut(s) 49
BmsI GCATC 4 cut(s) 291, 467, 509, 585
BpuMI CCSGG 1 cut(s) 49
BsaI GGTCTC 1 cut(s) 91
BsaJI CCNNGG 3 cut(s) 48, 290, 381
BsaWI WCCGGW 1 cut(s) 70
BsaXI ACNNNNNCTCC 2 cut(s) 100, 130
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 296, 448
Bse118I RCCGGY 1 cut(s) 70
BseDI CCNNGG 3 cut(s) 48, 290, 381
BseGI GGATG 4 cut(s) 18, 306, 440, 591
BseLI CCNNNNNNNGG 3 cut(s) 73, 296, 448
BseRI GAGGAG 1 cut(s) 413
BseXI GCAGC 1 cut(s) 282
BshFI GGCC 2 cut(s) 258, 404
BshNI GGYRCC 1 cut(s) 438
BshTI ACCGGT 1 cut(s) 70
BsiSI CCGG 2 cut(s) 48, 71
BslI CCNNNNNNNGG 3 cut(s) 73, 296, 448
BsmAI GTCTC 1 cut(s) 91
BsnI GGCC 2 cut(s) 258, 404
Bso31I GGTCTC 1 cut(s) 91
Bsp1286I GDGCHC 1 cut(s) 185
Bsp1407I TGTACA 1 cut(s) 564
Bsp143I GATC 3 cut(s) 125, 208, 231
BspACI CCGC 2 cut(s) 253, 389
BspANI GGCC 2 cut(s) 258, 404
BspLI GGNNCC 3 cut(s) 63, 257, 440
BspPI GGATC 1 cut(s) 120
BspT107I GGYRCC 1 cut(s) 438
BspTNI GGTCTC 1 cut(s) 91
BsrFI RCCGGY 1 cut(s) 70
BsrGI TGTACA 1 cut(s) 564
BssAI RCCGGY 1 cut(s) 70
BssECI CCNNGG 3 cut(s) 48, 290, 381
BssMI GATC 3 cut(s) 125, 208, 231
BssT1I CCWWGG 1 cut(s) 290
Bst4CI ACNGT 1 cut(s) 58
Bst6I CTCTTC 1 cut(s) 314
BstAUI TGTACA 1 cut(s) 564
BstC8I GCNNGC 2 cut(s) 260, 521
BstDEI CTNAG 1 cut(s) 156
BstENI CCTNNNNNAGG 1 cut(s) 294
BstF5I GGATG 4 cut(s) 18, 306, 440, 591
BstKTI GATC 3 cut(s) 128, 211, 234
BstMAI GTCTC 1 cut(s) 91
BstMBI GATC 3 cut(s) 125, 208, 231
BstMWI GCNNNNNNNGC 2 cut(s) 259, 301
BstNSI RCATGY 2 cut(s) 523, 530
BstSCI CCNGG 1 cut(s) 47
BstV1I GCAGC 1 cut(s) 282
BsuRI GGCC 2 cut(s) 258, 404
BtgZI GCGATG 1 cut(s) 467
BtsCI GGATG 4 cut(s) 18, 306, 440, 591
BtsIMutI CAGTG 1 cut(s) 63
Cac8I GCNNGC 2 cut(s) 260, 521
Cfr10I RCCGGY 1 cut(s) 70
Cfr13I GGNCC 1 cut(s) 256
Csp6I GTAC 1 cut(s) 565
CspAI ACCGGT 1 cut(s) 70
CviAII CATG 4 cut(s) 395, 520, 527, 602
CviQI GTAC 1 cut(s) 565
DdeI CTNAG 1 cut(s) 156
DpnI GATC 3 cut(s) 127, 210, 233
DpnII GATC 3 cut(s) 125, 208, 231
DraIII CACNNNGTG 1 cut(s) 509
Eam1104I CTCTTC 1 cut(s) 314
EarI CTCTTC 1 cut(s) 314
Eco130I CCWWGG 1 cut(s) 290
Eco24I GRGCYC 1 cut(s) 185
Eco31I GGTCTC 1 cut(s) 91
EcoNI CCTNNNNNAGG 1 cut(s) 294
EcoT14I CCWWGG 1 cut(s) 290
EcoT22I ATGCAT 1 cut(s) 502
EcoT38I GRGCYC 1 cut(s) 185
ErhI CCWWGG 1 cut(s) 290
FaeI CATG 4 cut(s) 398, 523, 530, 605
FaiI YATR 9 cut(s) 332, 396, 407, 498, 521, 528, 536, 569, 603
FatI CATG 4 cut(s) 394, 519, 526, 601
FauI CCCGC 1 cut(s) 246
Fnu4HI GCNGC 1 cut(s) 271
FokI GGATG 4 cut(s) 5, 313, 447, 598
FriOI GRGCYC 1 cut(s) 185
Fsp4HI GCNGC 1 cut(s) 271
FspBI CTAG 4 cut(s) 138, 291, 296, 607
GluI GCNGC 1 cut(s) 271
HaeIII GGCC 2 cut(s) 258, 404
HapII CCGG 2 cut(s) 48, 71
Hin1II CATG 4 cut(s) 398, 523, 530, 605
HincII GTYRAC 1 cut(s) 532
HindII GTYRAC 1 cut(s) 532
HinfI GANTC 2 cut(s) 109, 197
HpaII CCGG 2 cut(s) 48, 71
HphI GGTGA 3 cut(s) 81, 241, 379
Hpy166II GTNNAC 2 cut(s) 532, 565
Hpy188I TCNGA 3 cut(s) 130, 385, 463
Hpy8I GTNNAC 2 cut(s) 532, 565
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 2 cut(s) 145, 160
HpyCH4III ACNGT 1 cut(s) 58
HpyCH4IV ACGT 1 cut(s) 572
HpyCH4V TGCA 3 cut(s) 394, 500, 548
HpyF10VI GCNNNNNNNGC 2 cut(s) 259, 301
HpyF3I CTNAG 1 cut(s) 156
HpySE526I ACGT 1 cut(s) 572
Hsp92II CATG 4 cut(s) 398, 523, 530, 605
Kzo9I GATC 3 cut(s) 125, 208, 231
LmnI GCTCC 1 cut(s) 516
LpnPI CCDG 3 cut(s) 61, 84, 272
Lsp1109I GCAGC 1 cut(s) 282
LweI GCATC 4 cut(s) 291, 467, 509, 585
MaeI CTAG 4 cut(s) 138, 291, 296, 607
MaeII ACGT 1 cut(s) 572
MalI GATC 3 cut(s) 127, 210, 233
MboI GATC 3 cut(s) 125, 208, 231
MboII GAAGA 5 cut(s) 203, 331, 346, 364, 369
MhlI GDGCHC 1 cut(s) 185
MlyI GAGTC 1 cut(s) 118
MmeI TCCRAC 1 cut(s) 8
Mph1103I ATGCAT 1 cut(s) 502
MseI TTAA 1 cut(s) 539
MslI CAYNNNNRTG 2 cut(s) 505, 539
MspI CCGG 2 cut(s) 48, 71
MspR9I CCNGG 1 cut(s) 49
MwoI GCNNNNNNNGC 2 cut(s) 259, 301
NciI CCSGG 1 cut(s) 49
NdeII GATC 3 cut(s) 125, 208, 231
NlaIII CATG 4 cut(s) 398, 523, 530, 605
NlaIV GGNNCC 3 cut(s) 63, 257, 440
NsiI ATGCAT 1 cut(s) 502
NspI RCATGY 2 cut(s) 523, 530
PaeI GCATGC 1 cut(s) 523
PciI ACATGT 1 cut(s) 526
PfeI GAWTC 1 cut(s) 197
PinAI ACCGGT 1 cut(s) 70
PkrI GCNGC 1 cut(s) 272
PleI GAGTC 1 cut(s) 117
PpsI GAGTC 1 cut(s) 117
PscI ACATGT 1 cut(s) 526
PshBI ATTAAT 1 cut(s) 539
Psp1406I AACGTT 1 cut(s) 572
PspN4I GGNNCC 3 cut(s) 63, 257, 440
PspPI GGNCC 1 cut(s) 256
RsaI GTAC 1 cut(s) 566
RsaNI GTAC 1 cut(s) 565
RseI CAYNNNNRTG 2 cut(s) 505, 539
SaqAI TTAA 1 cut(s) 539
SatI GCNGC 1 cut(s) 271
Sau3AI GATC 3 cut(s) 125, 208, 231
Sau96I GGNCC 1 cut(s) 256
SchI GAGTC 1 cut(s) 118
ScrFI CCNGG 1 cut(s) 49
SduI GDGCHC 1 cut(s) 185
SetI ASST 8 cut(s) 94, 194, 326, 363, 420, 476, 557, 575
SfaNI GCATC 4 cut(s) 291, 467, 509, 585
SgrAI CRCCGGYG 1 cut(s) 70
SmiMI CAYNNNNRTG 2 cut(s) 505, 539
SphI GCATGC 1 cut(s) 523
SsiI CCGC 2 cut(s) 253, 389
SspMI CTAG 4 cut(s) 138, 291, 296, 607
StyD4I CCNGG 1 cut(s) 47
StyI CCWWGG 1 cut(s) 290
TaaI ACNGT 1 cut(s) 58
TaiI ACGT 1 cut(s) 575
TaqI TCGA 3 cut(s) 6, 179, 207
TaqII GACCGA 1 cut(s) 73
TatI WGTACW 1 cut(s) 564
TfiI GAWTC 1 cut(s) 197
Tru1I TTAA 1 cut(s) 539
Tru9I TTAA 1 cut(s) 539
TscAI CASTG 1 cut(s) 63
TseI GCWGC 1 cut(s) 270
TspDTI ATGAA 2 cut(s) 347, 363
TspGWI ACGGA 1 cut(s) 25
TspRI CASTG 1 cut(s) 63
VspI ATTAAT 1 cut(s) 539
XagI CCTNNNNNAGG 1 cut(s) 294
XceI RCATGY 2 cut(s) 523, 530
XmaJI CCTAGG 1 cut(s) 290
XspI CTAG 4 cut(s) 138, 291, 296, 607
Zsp2I ATGCAT 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.