Rroxscaffold_3G00253190

dual specificity protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0010404
Physical Location & Seq
Reverse (-)
47210878 .. 47214311
3434 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00253190.1

Sequence Viewer

Length: 978 bp
ATGTATATTGAGGAGTTGAAAGGTGGGGAAGGTGGAGAAGAAGTCGATTTAGGGCATGGCAGGGGCATCGTGGGTTCGGATGCGAAACGGGTCTTGATCGGAGCGGGGGCTCGTGCGCTGTTTTACCCGACTCTGCTTTACAATGTCATCAGGAATAAGCTTCAGGCTGAGTTTCGCTGGTGGGATAAGGTCGATGAGTTTGTATTGTTAGGTGCAGTTCCATTCCCTACTGATGTGCCTCGCCTAAAGGAGGTCGGAGTTCGTGGAGTCATCACACTTAATGAGCCATACGAGACTTTGGTTCCAACATCTCTTTATCAGTCCAATGGCATTAGCCATCTGGTGATCCCTACCAGAGACTACTGCTTTGCACCATCACTGAATGATATATGTCGTGCTGTAAACTTCATTAATGAAAATGCATCTCGTGGGACGACAACATATGTTCACTGTAAAGCTGGACGGGGCCGTAGTACGACCATTGTTATCTGCTACTTGGTCCATCACAAGCAGATGACACCTGATTCTGCCTATGAATATCTGAAGTCAATTCGGCCACGTGTGCTTTTGGCCTCTGCTCAGTGGCAGGCTGTTCAGGAGTACTACTATTTCATGGTGAAGAAAGCTGTGGTTTACAGCCACTTGACTAATCTAATATTGAATCCTCCATGTTCAGCTCCCTCACATGATCTTCTTGCATTTGACGAGGGCTCTGTAGTAGTGGTCACTGAATCAGATCTTGAAGGATATGATGCAAACCTCCAGTCTGGTACTGTGGGAAATGAGATATGGGCTGACTTAAGTGTGGTCTATCGGGTTCGAGTAGCTGGTCAGGCAGCTCTCACAAGGATTTCATGCCTGTGGCTCCGTTGCCATGCCCACCAGGAGAGTTCAGCTAAGAAGTTGGGAAGGGAAAGCAGCTGCTCGATTACAGCTGATCATCTGGGAGGCATTAGCGTGGACATACAAGTTTACTGA

Protein Analysis

325

Amino Acids

36.03

Weight (kDa)

6.37

Isoelectric Point (pI)

34.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTP-SAK PF22784 77 - 177 2.8e-14 Swiss Army Knife protein, DSP-PTPase phosphatase domain
Tc-R-P PF22785 80 - 170 1.6e-13 Polymorphic toxin system, DSP-PTPase phosphatase
DSPc PF00782 100 - 202 2.7e-18 Dual specificity phosphatase, catalytic domain
PTP-NADK PF22741 115 - 173 9e-06 DSP-PTPase phosphatase fused to NAD+ Kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 104
AciI CCGC 1 cut(s) 104
AclWI GGATC 1 cut(s) 340
AcoI YGGCCR 1 cut(s) 554
AcuI CTGAAG 2 cut(s) 146, 563
AcvI CACGTG 1 cut(s) 560
AfaI GTAC 3 cut(s) 475, 602, 772
AfiI CCNNNNNNNGG 1 cut(s) 250
AflII CTTAAG 1 cut(s) 799
AflIII ACRYGT 1 cut(s) 559
AgsI TTSAA 3 cut(s) 19, 661, 743
AjnI CCWGG 1 cut(s) 882
AluBI AGCT 9 cut(s) 160, 458, 626, 677, 827, 839, 896, 921, 935
AluI AGCT 9 cut(s) 160, 458, 626, 677, 827, 839, 896, 921, 935
Alw26I GTCTC 2 cut(s) 287, 351
AlwI GGATC 1 cut(s) 340
AoxI GGCC 3 cut(s) 466, 554, 570
ApeKI GCWGC 3 cut(s) 836, 918, 921
ArsI GACNNNNNNTTYG 4 cut(s) 350, 382, 749, 781
AseI ATTAAT 1 cut(s) 411
AspLEI GCGC 1 cut(s) 118
AspS9I GGNCC 2 cut(s) 466, 499
AsuHPI GGTGA 2 cut(s) 355, 628
AvaII GGWCC 1 cut(s) 499
BanII GRGCYC 2 cut(s) 112, 713
BauI CACGAG 2 cut(s) 111, 426
BbrPI CACGTG 1 cut(s) 560
BbvI GCAGC 3 cut(s) 848, 908, 930
BccI CCATC 3 cut(s) 345, 382, 510
BceAI ACGGC 1 cut(s) 453
BcgI CGANNNNNNTGC 2 cut(s) 49, 83
BciT130I CCWGG 1 cut(s) 884
BclI TGATCA 1 cut(s) 937
BcoDI GTCTC 2 cut(s) 287, 351
BfmI CTRYAG 1 cut(s) 714
BfrI CTTAAG 1 cut(s) 799
BglII AGATCT 1 cut(s) 736
BisI GCNGC 3 cut(s) 837, 919, 922
BlsI GCNGC 3 cut(s) 838, 920, 923
BmcAI AGTACT 1 cut(s) 602
Bme1390I CCNGG 1 cut(s) 884
Bme18I GGWCC 1 cut(s) 499
BmgT120I GGNCC 2 cut(s) 466, 499
BmiI GGNNCC 3 cut(s) 303, 467, 866
BmrFI CCNGG 1 cut(s) 884
BmsI GCATC 4 cut(s) 70, 75, 431, 742
BpmI CTGGAG 1 cut(s) 746
BsaAI YACGTR 1 cut(s) 560
BsaXI ACNNNNNCTCC 2 cut(s) 27, 57
Bsc4I CCNNNNNNNGG 1 cut(s) 250
Bse1I ACTGG 1 cut(s) 763
BseBI CCWGG 1 cut(s) 884
BseGI GGATG 1 cut(s) 85
BseLI CCNNNNNNNGG 1 cut(s) 250
BseMII CTCAG 2 cut(s) 159, 593
BseNI ACTGG 1 cut(s) 763
BseRI GAGGAG 1 cut(s) 26
BseXI GCAGC 3 cut(s) 848, 908, 930
BsgI GTGCAG 1 cut(s) 234
BshFI GGCC 3 cut(s) 468, 556, 572
BslFI GGGAC 1 cut(s) 445
BslI CCNNNNNNNGG 1 cut(s) 250
BsmAI GTCTC 2 cut(s) 287, 351
BsmFI GGGAC 1 cut(s) 445
BsnI GGCC 3 cut(s) 468, 556, 572
Bsp1286I GDGCHC 2 cut(s) 112, 713
Bsp143I GATC 5 cut(s) 96, 345, 688, 736, 937
BspACI CCGC 1 cut(s) 104
BspANI GGCC 3 cut(s) 468, 556, 572
BspCNI CTCAG 2 cut(s) 160, 592
BspLI GGNNCC 3 cut(s) 303, 467, 866
BspPI GGATC 1 cut(s) 340
BspTI CTTAAG 1 cut(s) 799
BsrBI CCGCTC 1 cut(s) 104
BsrI ACTGG 1 cut(s) 763
BssMI GATC 5 cut(s) 96, 345, 688, 736, 937
BssSI CACGAG 2 cut(s) 111, 426
Bst2BI CACGAG 2 cut(s) 111, 426
Bst2UI CCWGG 1 cut(s) 884
Bst4CI ACNGT 2 cut(s) 452, 775
BstAFI CTTAAG 1 cut(s) 799
BstBAI YACGTR 1 cut(s) 560
BstC8I GCNNGC 1 cut(s) 588
BstDEI CTNAG 3 cut(s) 168, 579, 897
BstENI CCTNNNNNAGG 1 cut(s) 248
BstF5I GGATG 1 cut(s) 85
BstHHI GCGC 1 cut(s) 118
BstKTI GATC 5 cut(s) 99, 348, 691, 739, 940
BstMAI GTCTC 2 cut(s) 287, 351
BstMBI GATC 5 cut(s) 96, 345, 688, 736, 937
BstMWI GCNNNNNNNGC 2 cut(s) 562, 833
BstNI CCWGG 1 cut(s) 884
BstSCI CCNGG 1 cut(s) 882
BstSFI CTRYAG 1 cut(s) 714
BstV1I GCAGC 3 cut(s) 848, 908, 930
BstX2I RGATCY 1 cut(s) 736
BstYI RGATCY 1 cut(s) 736
BsuRI GGCC 3 cut(s) 468, 556, 572
BtsCI GGATG 1 cut(s) 85
BtsIMutI CAGTG 4 cut(s) 377, 448, 587, 726
Cac8I GCNNGC 1 cut(s) 588
CfoI GCGC 1 cut(s) 118
Cfr13I GGNCC 2 cut(s) 466, 499
Csp6I GTAC 3 cut(s) 474, 601, 771
CviAII CATG 6 cut(s) 56, 613, 669, 686, 855, 875
CviQI GTAC 3 cut(s) 474, 601, 771
DdeI CTNAG 3 cut(s) 168, 579, 897
DpnI GATC 5 cut(s) 98, 347, 690, 738, 939
DpnII GATC 5 cut(s) 96, 345, 688, 736, 937
EaeI YGGCCR 1 cut(s) 554
Eco24I GRGCYC 2 cut(s) 112, 713
Eco47I GGWCC 1 cut(s) 499
Eco57I CTGAAG 2 cut(s) 146, 563
Eco72I CACGTG 1 cut(s) 560
EcoNI CCTNNNNNAGG 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 882
EcoT22I ATGCAT 1 cut(s) 424
EcoT38I GRGCYC 2 cut(s) 112, 713
FaeI CATG 6 cut(s) 59, 616, 672, 689, 858, 878
FaqI GGGAC 1 cut(s) 445
FatI CATG 6 cut(s) 55, 612, 668, 685, 854, 874
FauI CCCGC 1 cut(s) 97
FauNDI CATATG 1 cut(s) 442
FbaI TGATCA 1 cut(s) 937
Fnu4HI GCNGC 3 cut(s) 837, 919, 922
FokI GGATG 1 cut(s) 92
FriOI GRGCYC 2 cut(s) 112, 713
Fsp4HI GCNGC 3 cut(s) 837, 919, 922
GlaI GCGC 1 cut(s) 117
GluI GCNGC 3 cut(s) 837, 919, 922
GsuI CTGGAG 1 cut(s) 746
HaeIII GGCC 3 cut(s) 468, 556, 572
HhaI GCGC 1 cut(s) 118
Hin1II CATG 6 cut(s) 59, 616, 672, 689, 858, 878
Hin6I GCGC 1 cut(s) 116
HinP1I GCGC 1 cut(s) 116
HindIII AAGCTT 1 cut(s) 158
HinfI GANTC 5 cut(s) 130, 267, 524, 661, 731
HphI GGTGA 2 cut(s) 355, 628
Hpy166II GTNNAC 5 cut(s) 403, 448, 634, 961, 973
Hpy188I TCNGA 5 cut(s) 79, 101, 257, 543, 736
Hpy188III TCNNGA 4 cut(s) 94, 151, 596, 740
Hpy8I GTNNAC 5 cut(s) 403, 448, 634, 961, 973
HpyAV CCTTC 3 cut(s) 23, 737, 903
HpyCH4III ACNGT 2 cut(s) 452, 775
HpyCH4IV ACGT 1 cut(s) 559
HpyCH4V TGCA 5 cut(s) 215, 371, 422, 698, 755
HpyF10VI GCNNNNNNNGC 2 cut(s) 562, 833
HpyF3I CTNAG 3 cut(s) 168, 579, 897
HpySE526I ACGT 1 cut(s) 559
Hsp92II CATG 6 cut(s) 59, 616, 672, 689, 858, 878
HspAI GCGC 1 cut(s) 116
Ksp22I TGATCA 1 cut(s) 937
Kzo9I GATC 5 cut(s) 96, 345, 688, 736, 937
LmnI GCTCC 3 cut(s) 101, 682, 870
Lsp1109I GCAGC 3 cut(s) 848, 908, 930
LweI GCATC 4 cut(s) 70, 75, 431, 742
MaeII ACGT 1 cut(s) 559
MaeIII GTNAC 1 cut(s) 724
MalI GATC 5 cut(s) 98, 347, 690, 738, 939
MbiI CCGCTC 1 cut(s) 104
MboI GATC 5 cut(s) 96, 345, 688, 736, 937
MboII GAAGA 3 cut(s) 50, 631, 683
MflI RGATCY 1 cut(s) 736
MhlI GDGCHC 2 cut(s) 112, 713
MluCI AATT 1 cut(s) 549
MlyI GAGTC 2 cut(s) 124, 276
MmeI TCCRAC 2 cut(s) 235, 329
MnlI CCTC 9 cut(s) 4, 244, 249, 583, 675, 691, 700, 770, 941
Mph1103I ATGCAT 1 cut(s) 424
MseI TTAA 3 cut(s) 279, 411, 800
MslI CAYNNNNRTG 2 cut(s) 859, 956
MspA1I CMGCKG 2 cut(s) 921, 935
MspCI CTTAAG 1 cut(s) 799
MspR9I CCNGG 1 cut(s) 884
MvaI CCWGG 1 cut(s) 884
MwoI GCNNNNNNNGC 2 cut(s) 562, 833
NdeI CATATG 1 cut(s) 442
NdeII GATC 5 cut(s) 96, 345, 688, 736, 937
NlaIII CATG 6 cut(s) 59, 616, 672, 689, 858, 878
NlaIV GGNNCC 3 cut(s) 303, 467, 866
NmuCI GTSAC 1 cut(s) 724
NsiI ATGCAT 1 cut(s) 424
PfeI GAWTC 3 cut(s) 524, 661, 731
PkrI GCNGC 3 cut(s) 838, 920, 923
PleI GAGTC 2 cut(s) 124, 275
PmaCI CACGTG 1 cut(s) 560
PmlI CACGTG 1 cut(s) 560
PpsI GAGTC 2 cut(s) 124, 275
Ppu21I YACGTR 1 cut(s) 560
PshBI ATTAAT 1 cut(s) 411
Psp6I CCWGG 1 cut(s) 882
PspCI CACGTG 1 cut(s) 560
PspGI CCWGG 1 cut(s) 882
PspN4I GGNNCC 3 cut(s) 303, 467, 866
PspPI GGNCC 2 cut(s) 466, 499
PsuI RGATCY 1 cut(s) 736
PvuII CAGCTG 2 cut(s) 921, 935
RsaI GTAC 3 cut(s) 475, 602, 772
RsaNI GTAC 3 cut(s) 474, 601, 771
RseI CAYNNNNRTG 2 cut(s) 859, 956
SaqAI TTAA 3 cut(s) 279, 411, 800
SatI GCNGC 3 cut(s) 837, 919, 922
Sau3AI GATC 5 cut(s) 96, 345, 688, 736, 937
Sau96I GGNCC 2 cut(s) 466, 499
ScaI AGTACT 1 cut(s) 602
SchI GAGTC 2 cut(s) 124, 276
ScrFI CCNGG 1 cut(s) 884
SduI GDGCHC 2 cut(s) 112, 713
SfaNI GCATC 4 cut(s) 70, 75, 431, 742
SfcI CTRYAG 1 cut(s) 714
SinI GGWCC 1 cut(s) 499
SmiMI CAYNNNNRTG 2 cut(s) 859, 956
SmlI CTYRAG 1 cut(s) 799
SmoI CTYRAG 1 cut(s) 799
Sse9I AATT 1 cut(s) 549
SsiI CCGC 1 cut(s) 104
SspI AATATT 1 cut(s) 657
StyD4I CCNGG 1 cut(s) 882
TaaI ACNGT 2 cut(s) 452, 775
TaiI ACGT 1 cut(s) 562
TaqI TCGA 4 cut(s) 45, 192, 820, 926
TasI AATT 1 cut(s) 549
TatI WGTACW 1 cut(s) 600
TfiI GAWTC 3 cut(s) 524, 661, 731
Tru1I TTAA 3 cut(s) 279, 411, 800
Tru9I TTAA 3 cut(s) 279, 411, 800
TscAI CASTG 4 cut(s) 384, 455, 587, 733
TseFI GTSAC 1 cut(s) 724
TseI GCWGC 3 cut(s) 836, 918, 921
Tsp45I GTSAC 1 cut(s) 724
TspDTI ATGAA 5 cut(s) 397, 429, 549, 601, 843
TspGWI ACGGA 1 cut(s) 857
TspRI CASTG 4 cut(s) 384, 455, 587, 733
Vha464I CTTAAG 1 cut(s) 799
VpaK11BI GGWCC 1 cut(s) 499
VspI ATTAAT 1 cut(s) 411
XagI CCTNNNNNAGG 1 cut(s) 248
ZrmI AGTACT 1 cut(s) 602
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.