Rroxscaffold_4G00286520

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0007676
Physical Location & Seq
Reverse (-)
7501853 .. 7505225
3373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00286520.1

Sequence Viewer

Length: 1143 bp
ATGCCTTGCGCTTTAGCGGTACCAAACTCTCCGATCTTCTCACCGTCGAAAATTCCGTCCATTTTCTGCCGGTCATCTCCTTCTTCATGTTCTTCTCCTAGAGTACTTCTCCATGGTCCACCTCCCACGCCGTCGTCACGGTCCAACAACCCACTTTCGTCGCCGAAGGCTTTCCCGCCAAGGTTTCAGAGTCAGAGGCATGTGATTAGTAACTGCAAGAAGGAGCCGTCGGGTTTGGGCTCGGGTCCGGGTTTGACTTTGAAGAGGAAGAGGCCGATGAGGATTGAGATTCCGACTGCGCCGGTGAGTTTCGGCGTTGACTCGCCGAAGGTTGAGGAGAGAGTGGAGACGGTGGAGGTTGACGAAGATGGATACTCGGTTTACTGTAAGAGAGGGAAAAGAGGTCTCATGGAGGATCGGTACTCCGCCGTGGTTGATCTTCATGATAATACCAGACAGGCGTTCTTTGGTGTGTTTGATGGGCATGGAGGTTCAAAGGCAGCTGAATTTGCAGCAAAGAACTTGGATAAGAACATCGTGGGTCGTTTGGCGAGTGGAGGAGAAGAGAAGGTCATGGAAGCAGTGAAAGATGGTTATTTGGCGACTGATGTTGAGTTTTTGAAAGAGGATGTTAGTGGTGGTGCTTGCTGTGTGACTGCTTTGGTACAGAAAGGTAATCTGGTGGTGTCAAATGCTGGTGATTGCCGCGCTGTTTTGAGCCGAGGAGGGGTTGCTGAGGCCCTTACGTCTGATCATCATCCTTGTAGACCAGATGAAAGAGCCAGAATTGAAAGCTCGGGTGGTTATGTGGATTATTGCCGCGGTGTTTGGAGAATACAGGGGTCTCTTGCTGTTTCAAGAGGAATTGGAGATCGACAGCTAAAGCAGTGGGTTATAGCAGAACCAGAGACTCAAGTCCTAAAAATCAATCCTGAATGCGAGTTCTTAATGTTAGCTTCTGACGGTCTTTGGGACAAGGTTACTAATCAAGAGGCAGTTGACGTAGTTCGCCCTCTATGCGTTGGAATTGATAAACCGGAGCTGTTTTCTGCTTGTAAAAAGCTTGTTGATCTTTCAATAAGTAGAGGTTCCATGGACGATACAAGCGTGATGATAATCCAATTAGGCCGTTTTGTTTCGTAA

Protein Analysis

380

Amino Acids

41.11

Weight (kDa)

7.91

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 126 - 367 8.6e-59 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 19
AccB1I GGYRCC 1 cut(s) 19
AccI GTMKAC 1 cut(s) 766
AccII CGCG 2 cut(s) 708, 822
AciI CCGC 6 cut(s) 17, 176, 426, 706, 820, 822
AclWI GGATC 1 cut(s) 423
AcsI RAATTY 2 cut(s) 51, 506
AfaI GTAC 4 cut(s) 21, 105, 422, 666
AfiI CCNNNNNNNGG 1 cut(s) 727
AgsI TTSAA 6 cut(s) 262, 495, 622, 791, 858, 1077
AluBI AGCT 6 cut(s) 503, 795, 880, 956, 1042, 1063
AluI AGCT 6 cut(s) 503, 795, 880, 956, 1042, 1063
Alw26I GTCTC 4 cut(s) 341, 410, 849, 902
AlwI GGATC 1 cut(s) 423
Ama87I CYCGRG 2 cut(s) 241, 796
AoxI GGCC 3 cut(s) 272, 738, 1126
ApeKI GCWGC 2 cut(s) 500, 512
ApoI RAATTY 2 cut(s) 51, 506
ArsI GACNNNNNNTTYG 2 cut(s) 41, 73
Asp700I GAANNNNTTC 1 cut(s) 170
Asp718I GGTACC 1 cut(s) 19
AspLEI GCGC 3 cut(s) 11, 301, 710
AspS9I GGNCC 4 cut(s) 116, 141, 245, 739
AsuC2I CCSGG 1 cut(s) 249
AsuHPI GGTGA 3 cut(s) 33, 316, 710
AvaI CYCGRG 2 cut(s) 241, 796
AvaII GGWCC 3 cut(s) 116, 141, 245
BaeI ACNNNNGTAYC 2 cut(s) 1092, 1125
BanI GGYRCC 1 cut(s) 19
BanII GRGCYC 1 cut(s) 242
BbvCI CCTCAGC 1 cut(s) 735
BbvI GCAGC 2 cut(s) 512, 524
BccI CCATC 3 cut(s) 362, 473, 584
BceAI ACGGC 4 cut(s) 115, 211, 413, 1113
BciVI GTATCC 1 cut(s) 365
BclI TGATCA 1 cut(s) 751
BcnI CCSGG 1 cut(s) 249
BcoDI GTCTC 4 cut(s) 341, 410, 849, 902
BfaI CTAG 1 cut(s) 99
BfuI GTATCC 1 cut(s) 365
BisI GCNGC 4 cut(s) 501, 513, 706, 820
BlsI GCNGC 4 cut(s) 502, 514, 707, 821
BmcAI AGTACT 1 cut(s) 105
Bme1390I CCNGG 1 cut(s) 249
Bme18I GGWCC 3 cut(s) 116, 141, 245
BmeT110I CYCGRG 2 cut(s) 241, 796
BmgT120I GGNCC 4 cut(s) 116, 141, 245, 739
BmiI GGNNCC 4 cut(s) 21, 225, 246, 1090
BmrFI CCNGG 1 cut(s) 249
BoxI GACNNNNGTC 1 cut(s) 914
BplI GAGNNNNNCTC 2 cut(s) 93, 125
Bpu10I CCTNAGC 1 cut(s) 735
BpuEI CTTGAG 1 cut(s) 897
BpuMI CCSGG 1 cut(s) 249
BsaBI GATNNNNATC 2 cut(s) 756, 1115
BsaI GGTCTC 2 cut(s) 410, 849
BsaJI CCNNGG 6 cut(s) 112, 179, 429, 721, 820, 1092
BsaWI WCCGGW 1 cut(s) 1036
BsaXI ACNNNNNCTCC 2 cut(s) 404, 434
Bsc4I CCNNNNNNNGG 1 cut(s) 727
Bse118I RCCGGY 2 cut(s) 69, 301
Bse8I GATNNNNATC 2 cut(s) 756, 1115
BseDI CCNNGG 6 cut(s) 112, 179, 429, 721, 820, 1092
BseGI GGATG 2 cut(s) 634, 757
BseJI GATNNNNATC 2 cut(s) 756, 1115
BseLI CCNNNNNNNGG 1 cut(s) 727
BseMII CTCAG 1 cut(s) 726
BseRI GAGGAG 3 cut(s) 350, 573, 738
BseXI GCAGC 2 cut(s) 512, 524
Bsh1236I CGCG 2 cut(s) 708, 822
BshFI GGCC 3 cut(s) 274, 740, 1128
BshNI GGYRCC 1 cut(s) 19
BsiHKCI CYCGRG 2 cut(s) 241, 796
BsiSI CCGG 4 cut(s) 70, 248, 302, 1037
BslFI GGGAC 1 cut(s) 986
BslI CCNNNNNNNGG 1 cut(s) 727
BsmAI GTCTC 4 cut(s) 341, 410, 849, 902
BsmBI CGTCTC 1 cut(s) 341
BsmFI GGGAC 1 cut(s) 986
BsmI GAATGC 1 cut(s) 941
BsnI GGCC 3 cut(s) 274, 740, 1128
Bso31I GGTCTC 2 cut(s) 410, 849
BsoBI CYCGRG 2 cut(s) 241, 796
Bsp1286I GDGCHC 1 cut(s) 242
Bsp143I GATC 6 cut(s) 33, 415, 436, 751, 871, 1069
Bsp19I CCATGG 2 cut(s) 112, 1092
BspACI CCGC 6 cut(s) 17, 176, 426, 706, 820, 822
BspANI GGCC 3 cut(s) 274, 740, 1128
BspCNI CTCAG 1 cut(s) 727
BspFNI CGCG 2 cut(s) 708, 822
BspHI TCATGA 1 cut(s) 442
BspLI GGNNCC 4 cut(s) 21, 225, 246, 1090
BspPI GGATC 1 cut(s) 423
BspT107I GGYRCC 1 cut(s) 19
BspTNI GGTCTC 2 cut(s) 410, 849
BsrFI RCCGGY 2 cut(s) 69, 301
BssAI RCCGGY 2 cut(s) 69, 301
BssECI CCNNGG 6 cut(s) 112, 179, 429, 721, 820, 1092
BssMI GATC 6 cut(s) 33, 415, 436, 751, 871, 1069
BssT1I CCWWGG 3 cut(s) 112, 179, 1092
Bst4CI ACNGT 5 cut(s) 45, 141, 352, 386, 965
Bst6I CTCTTC 3 cut(s) 257, 263, 558
BstC8I GCNNGC 1 cut(s) 646
BstDEI CTNAG 1 cut(s) 735
BstDSI CCRYGG 4 cut(s) 112, 429, 820, 1092
BstF5I GGATG 2 cut(s) 634, 757
BstFNI CGCG 2 cut(s) 708, 822
BstHHI GCGC 3 cut(s) 11, 301, 710
BstKTI GATC 6 cut(s) 36, 418, 439, 754, 874, 1072
BstMAI GTCTC 4 cut(s) 341, 410, 849, 902
BstMBI GATC 6 cut(s) 33, 415, 436, 751, 871, 1069
BstMWI GCNNNNNNNGC 2 cut(s) 509, 1017
BstNSI RCATGY 1 cut(s) 203
BstPAI GACNNNNGTC 1 cut(s) 914
BstSCI CCNGG 1 cut(s) 247
BstUI CGCG 2 cut(s) 708, 822
BstV1I GCAGC 2 cut(s) 512, 524
BsuI GTATCC 1 cut(s) 365
BsuRI GGCC 3 cut(s) 274, 740, 1128
BtgI CCRYGG 4 cut(s) 112, 429, 820, 1092
BtsCI GGATG 2 cut(s) 634, 757
BtsI GCAGTG 2 cut(s) 588, 893
BtsIMutI CAGTG 2 cut(s) 588, 893
Cac8I GCNNGC 1 cut(s) 646
CciI TCATGA 1 cut(s) 442
CfoI GCGC 3 cut(s) 11, 301, 710
Cfr10I RCCGGY 2 cut(s) 69, 301
Cfr13I GGNCC 4 cut(s) 116, 141, 245, 739
Cfr42I CCGCGG 1 cut(s) 823
Csp6I GTAC 4 cut(s) 20, 104, 421, 665
CviAII CATG 8 cut(s) 87, 113, 200, 409, 443, 485, 574, 1093
CviQI GTAC 4 cut(s) 20, 104, 421, 665
DdeI CTNAG 1 cut(s) 735
DpnI GATC 6 cut(s) 35, 417, 438, 753, 873, 1071
DpnII GATC 6 cut(s) 33, 415, 436, 751, 871, 1069
Eam1104I CTCTTC 3 cut(s) 257, 263, 558
EarI CTCTTC 3 cut(s) 257, 263, 558
EciI GGCGGA 1 cut(s) 415
Eco130I CCWWGG 3 cut(s) 112, 179, 1092
Eco24I GRGCYC 1 cut(s) 242
Eco31I GGTCTC 2 cut(s) 410, 849
Eco47I GGWCC 3 cut(s) 116, 141, 245
Eco88I CYCGRG 2 cut(s) 241, 796
EcoO109I RGGNCCY 1 cut(s) 739
EcoT14I CCWWGG 3 cut(s) 112, 179, 1092
EcoT38I GRGCYC 1 cut(s) 242
ErhI CCWWGG 3 cut(s) 112, 179, 1092
Esp3I CGTCTC 1 cut(s) 341
FaeI CATG 8 cut(s) 90, 116, 203, 412, 446, 488, 577, 1096
FaqI GGGAC 1 cut(s) 986
FatI CATG 8 cut(s) 86, 112, 199, 408, 442, 484, 573, 1092
FauI CCCGC 1 cut(s) 183
FbaI TGATCA 1 cut(s) 751
FblI GTMKAC 1 cut(s) 766
Fnu4HI GCNGC 4 cut(s) 501, 513, 706, 820
FokI GGATG 2 cut(s) 641, 744
FriOI GRGCYC 1 cut(s) 242
Fsp4HI GCNGC 4 cut(s) 501, 513, 706, 820
FspBI CTAG 1 cut(s) 99
GlaI GCGC 3 cut(s) 10, 300, 709
GluI GCNGC 4 cut(s) 501, 513, 706, 820
HaeIII GGCC 3 cut(s) 274, 740, 1128
HapII CCGG 4 cut(s) 70, 248, 302, 1037
HhaI GCGC 3 cut(s) 11, 301, 710
Hin1II CATG 8 cut(s) 90, 116, 203, 412, 446, 488, 577, 1096
Hin6I GCGC 3 cut(s) 9, 299, 708
HinP1I GCGC 3 cut(s) 9, 299, 708
HincII GTYRAC 3 cut(s) 319, 361, 1000
HindII GTYRAC 3 cut(s) 319, 361, 1000
HindIII AAGCTT 1 cut(s) 1061
HinfI GANTC 4 cut(s) 190, 289, 320, 910
HpaII CCGG 4 cut(s) 70, 248, 302, 1037
HphI GGTGA 3 cut(s) 33, 316, 710
Hpy166II GTNNAC 6 cut(s) 119, 319, 361, 382, 767, 1000
Hpy188I TCNGA 6 cut(s) 33, 189, 195, 294, 751, 961
Hpy188III TCNNGA 4 cut(s) 443, 858, 932, 989
Hpy8I GTNNAC 6 cut(s) 119, 319, 361, 382, 767, 1000
Hpy99I CGWCG 4 cut(s) 49, 136, 163, 232
HpyAV CCTTC 5 cut(s) 90, 160, 214, 322, 562
HpyCH4III ACNGT 5 cut(s) 45, 141, 352, 386, 965
HpyCH4IV ACGT 2 cut(s) 746, 1002
HpyCH4V TGCA 2 cut(s) 216, 512
HpyF10VI GCNNNNNNNGC 2 cut(s) 509, 1017
HpyF3I CTNAG 1 cut(s) 735
HpySE526I ACGT 2 cut(s) 746, 1002
Hsp92II CATG 8 cut(s) 90, 116, 203, 412, 446, 488, 577, 1096
HspAI GCGC 3 cut(s) 9, 299, 708
KpnI GGTACC 1 cut(s) 23
Ksp22I TGATCA 1 cut(s) 751
KspI CCGCGG 1 cut(s) 823
Kzo9I GATC 6 cut(s) 33, 415, 436, 751, 871, 1069
LmnI GCTCC 2 cut(s) 223, 1039
Lsp1109I GCAGC 2 cut(s) 512, 524
MaeI CTAG 1 cut(s) 99
MaeII ACGT 2 cut(s) 746, 1002
MaeIII GTNAC 4 cut(s) 135, 209, 652, 979
MalI GATC 6 cut(s) 35, 417, 438, 753, 873, 1071
MboI GATC 6 cut(s) 33, 415, 436, 751, 871, 1069
MboII GAAGA 8 cut(s) 28, 75, 84, 274, 280, 377, 431, 575
MhlI GDGCHC 1 cut(s) 242
MluCI AATT 6 cut(s) 51, 506, 786, 864, 1026, 1121
MlyI GAGTC 3 cut(s) 199, 314, 904
MmeI TCCRAC 3 cut(s) 168, 317, 1003
MroXI GAANNNNTTC 1 cut(s) 170
MseI TTAA 1 cut(s) 947
MspA1I CMGCKG 2 cut(s) 503, 822
MspI CCGG 4 cut(s) 70, 248, 302, 1037
MspR9I CCNGG 1 cut(s) 249
Mva1269I GAATGC 1 cut(s) 941
MvnI CGCG 2 cut(s) 708, 822
MwoI GCNNNNNNNGC 2 cut(s) 509, 1017
NciI CCSGG 1 cut(s) 249
NcoI CCATGG 2 cut(s) 112, 1092
NdeII GATC 6 cut(s) 33, 415, 436, 751, 871, 1069
NlaIII CATG 8 cut(s) 90, 116, 203, 412, 446, 488, 577, 1096
NlaIV GGNNCC 4 cut(s) 21, 225, 246, 1090
NmeAIII GCCGAG 1 cut(s) 746
NmuCI GTSAC 2 cut(s) 135, 652
NspI RCATGY 1 cut(s) 203
PagI TCATGA 1 cut(s) 442
PcsI WCGNNNNNNNCGW 2 cut(s) 53, 1104
PctI GAATGC 1 cut(s) 941
PdmI GAANNNNTTC 1 cut(s) 170
PfeI GAWTC 1 cut(s) 289
PkrI GCNGC 4 cut(s) 502, 514, 707, 821
PleI GAGTC 3 cut(s) 198, 314, 904
PpsI GAGTC 3 cut(s) 198, 314, 904
PshAI GACNNNNGTC 1 cut(s) 914
PspN4I GGNNCC 4 cut(s) 21, 225, 246, 1090
PspPI GGNCC 4 cut(s) 116, 141, 245, 739
PvuII CAGCTG 1 cut(s) 503
RsaI GTAC 4 cut(s) 21, 105, 422, 666
RsaNI GTAC 4 cut(s) 20, 104, 421, 665
SacII CCGCGG 1 cut(s) 823
SaqAI TTAA 1 cut(s) 947
SatI GCNGC 4 cut(s) 501, 513, 706, 820
Sau3AI GATC 6 cut(s) 33, 415, 436, 751, 871, 1069
Sau96I GGNCC 4 cut(s) 116, 141, 245, 739
ScaI AGTACT 1 cut(s) 105
SchI GAGTC 3 cut(s) 199, 314, 904
ScrFI CCNGG 1 cut(s) 249
SduI GDGCHC 1 cut(s) 242
Sfr303I CCGCGG 1 cut(s) 823
SgrAI CRCCGGYG 1 cut(s) 301
SgrBI CCGCGG 1 cut(s) 823
SinI GGWCC 3 cut(s) 116, 141, 245
SmlI CTYRAG 1 cut(s) 912
SmoI CTYRAG 1 cut(s) 912
Sse9I AATT 6 cut(s) 51, 506, 786, 864, 1026, 1121
SsiI CCGC 6 cut(s) 17, 176, 426, 706, 820, 822
SspMI CTAG 1 cut(s) 99
StyD4I CCNGG 1 cut(s) 247
StyI CCWWGG 3 cut(s) 112, 179, 1092
TaaI ACNGT 5 cut(s) 45, 141, 352, 386, 965
TaiI ACGT 2 cut(s) 749, 1005
TaqI TCGA 2 cut(s) 47, 874
TasI AATT 6 cut(s) 51, 506, 786, 864, 1026, 1121
TatI WGTACW 1 cut(s) 103
TauI GCSGC 2 cut(s) 708, 822
TfiI GAWTC 1 cut(s) 289
Tru1I TTAA 1 cut(s) 947
Tru9I TTAA 1 cut(s) 947
TscAI CASTG 2 cut(s) 588, 893
TseFI GTSAC 2 cut(s) 135, 652
TseI GCWGC 2 cut(s) 500, 512
Tsp45I GTSAC 2 cut(s) 135, 652
TspDTI ATGAA 3 cut(s) 75, 431, 789
TspGWI ACGGA 1 cut(s) 45
TspRI CASTG 2 cut(s) 588, 893
VpaK11BI GGWCC 3 cut(s) 116, 141, 245
XapI RAATTY 2 cut(s) 51, 506
XceI RCATGY 1 cut(s) 203
XmiI GTMKAC 1 cut(s) 766
XmnI GAANNNNTTC 1 cut(s) 170
XspI CTAG 1 cut(s) 99
ZrmI AGTACT 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.