Rw1G032220

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0007676
Physical Location & Seq
Forward (+)
60553006 .. 60554579
1574 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G032220.1

Sequence Viewer

Length: 1143 bp
ATGCCTTGCGCTTTAGCGGTACCAAACTCTCCGATCTTCTCACCGTCGAAAATTCCGTCCATTTTCTGCCGGTCATCTCCTTCTTCATGTTCTTCTCCTAGAGTACTTCTCCATGGTCCACCTCCAACGCCGTCGTCACGGTCCAACAACCCACTTTCGTCGCCGAAGGCTTTCCCGGCAAGGTTTCAGAGTCAGAGGCATGTGATTAGTAACTGCAAGAAGGAGCCGTCGGGTTTGGGCTCGGGTCCGGGTTTGGCTTTGAAGAGGAAGAGGCCGATGAGGATTGATATTCCGACTGCGCCGGTGAGTTTCGGCGTTGACTCGCCGAAGGTTGAGGAGAGAGTGGAGGCGGTGGAGGTTGACGAAGATGGTTACTCGGTTTACTGTAAGAGAGGGAAAAGAGGTCTCATGGAGGATCGGTACTCCGCCGTGGTTGATCTTCATGATAATACCAGACAGGCGTTCTTTGGTGTGTTTGATGGGCATGGAGGTTCAAAGGCAGCTGAATTTGCAGCAAAGAACTTGGATAAGAACATCGTGCGTCGTTTGGCGAGTGGAGGAGAAGAGAAGGTCATGGAAGCAGTGAAAGATGGTTATTTGGCGACCGATGTTGAGTTTTTGAAAGAGGATGTTAGTGGTGGTGCTTGCTGTGTGACTGCCTTGGTACAGAAAGGTAACCTGGTGGTGTCGAATGCTGGTGATTGCCGCGCTGTTATGAGCCGAGGAGGGGTTGCTGAGGCCCTTACGTCTGATCATCATCCTAGTAGAACAGATGAAAGAGCCAGACTTGAAAGCTCGGGTGGTTATGTGGATTGTTGCCGCGGTGTTTGGAGAATACAAGGGTCTCTTGCTGTTTCAAGAGGAATTGGAGATCGACAGCTAAAGCAGTGGGTTATAGCAGAACCAGAGACTCAAGTCCTAAAAATAAATCCTGAATGCGAGTTCTTAATGTTAGCTTCTGACGGTCTTTGGGACAAGGTTACTAATCAAGAGGCAGTTGATGTAGTTCGCCCTCTATGCGTTGGAATTGATAAGCTGGAGCTGTTTTCTGCTTGTAAAAAGCTTGTTGATCTTTCAATAAGTAGAGGTTCCATGGATGATACAAGCGTGATGATAATCCAATTAGGCCGTTTTGTTTCGTAA

Protein Analysis

380

Amino Acids

41.07

Weight (kDa)

8.18

Isoelectric Point (pI)

43.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 126 - 367 5e-59 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 19
AccB1I GGYRCC 1 cut(s) 19
AccII CGCG 2 cut(s) 708, 822
AciI CCGC 6 cut(s) 17, 350, 426, 706, 820, 822
AclWI GGATC 1 cut(s) 423
AcsI RAATTY 2 cut(s) 51, 506
AfaI GTAC 4 cut(s) 21, 105, 422, 666
AfiI CCNNNNNNNGG 1 cut(s) 727
AgsI TTSAA 6 cut(s) 262, 495, 622, 791, 858, 1077
AjnI CCWGG 1 cut(s) 678
AluBI AGCT 7 cut(s) 503, 795, 880, 956, 1036, 1042, 1063
AluI AGCT 7 cut(s) 503, 795, 880, 956, 1036, 1042, 1063
Alw26I GTCTC 3 cut(s) 410, 849, 902
AlwI GGATC 1 cut(s) 423
Ama87I CYCGRG 2 cut(s) 241, 796
AoxI GGCC 3 cut(s) 272, 738, 1126
ApeKI GCWGC 2 cut(s) 500, 512
ApoI RAATTY 2 cut(s) 51, 506
ArsI GACNNNNNNTTYG 2 cut(s) 41, 73
Asp700I GAANNNNTTC 1 cut(s) 170
Asp718I GGTACC 1 cut(s) 19
AspLEI GCGC 3 cut(s) 11, 301, 710
AspS9I GGNCC 4 cut(s) 116, 141, 245, 739
AsuC2I CCSGG 2 cut(s) 176, 249
AsuHPI GGTGA 3 cut(s) 33, 316, 710
AvaI CYCGRG 2 cut(s) 241, 796
AvaII GGWCC 3 cut(s) 116, 141, 245
BaeI ACNNNNGTAYC 2 cut(s) 1092, 1125
BanI GGYRCC 1 cut(s) 19
BanII GRGCYC 1 cut(s) 242
BbvCI CCTCAGC 1 cut(s) 735
BbvI GCAGC 2 cut(s) 512, 524
BccI CCATC 3 cut(s) 362, 473, 584
BceAI ACGGC 4 cut(s) 115, 211, 413, 1113
BciT130I CCWGG 1 cut(s) 680
BclI TGATCA 1 cut(s) 751
BcnI CCSGG 2 cut(s) 176, 249
BcoDI GTCTC 3 cut(s) 410, 849, 902
BfaI CTAG 2 cut(s) 99, 762
BisI GCNGC 4 cut(s) 501, 513, 706, 820
BlsI GCNGC 4 cut(s) 502, 514, 707, 821
BmcAI AGTACT 1 cut(s) 105
Bme1390I CCNGG 3 cut(s) 176, 249, 680
Bme18I GGWCC 3 cut(s) 116, 141, 245
BmeT110I CYCGRG 2 cut(s) 241, 796
BmgT120I GGNCC 4 cut(s) 116, 141, 245, 739
BmiI GGNNCC 4 cut(s) 21, 225, 246, 1090
BmrFI CCNGG 3 cut(s) 176, 249, 680
BoxI GACNNNNGTC 1 cut(s) 914
BplI GAGNNNNNCTC 2 cut(s) 93, 125
BpmI CTGGAG 1 cut(s) 1058
Bpu10I CCTNAGC 1 cut(s) 735
BpuEI CTTGAG 1 cut(s) 897
BpuMI CCSGG 2 cut(s) 176, 249
BsaBI GATNNNNATC 2 cut(s) 756, 1115
BsaI GGTCTC 2 cut(s) 410, 849
BsaJI CCNNGG 6 cut(s) 112, 429, 660, 721, 820, 1092
BsaXI ACNNNNNCTCC 2 cut(s) 404, 434
Bsc4I CCNNNNNNNGG 1 cut(s) 727
Bse118I RCCGGY 2 cut(s) 69, 301
Bse8I GATNNNNATC 2 cut(s) 756, 1115
BseBI CCWGG 1 cut(s) 680
BseDI CCNNGG 6 cut(s) 112, 429, 660, 721, 820, 1092
BseGI GGATG 3 cut(s) 634, 757, 1102
BseJI GATNNNNATC 2 cut(s) 756, 1115
BseLI CCNNNNNNNGG 1 cut(s) 727
BseMII CTCAG 1 cut(s) 726
BseRI GAGGAG 3 cut(s) 350, 573, 738
BseXI GCAGC 2 cut(s) 512, 524
Bsh1236I CGCG 2 cut(s) 708, 822
Bsh1285I CGRYCG 1 cut(s) 606
BshFI GGCC 3 cut(s) 274, 740, 1128
BshNI GGYRCC 1 cut(s) 19
BsiEI CGRYCG 1 cut(s) 606
BsiHKCI CYCGRG 2 cut(s) 241, 796
BsiSI CCGG 4 cut(s) 70, 176, 248, 302
BslFI GGGAC 1 cut(s) 986
BslI CCNNNNNNNGG 1 cut(s) 727
BsmAI GTCTC 3 cut(s) 410, 849, 902
BsmFI GGGAC 1 cut(s) 986
BsmI GAATGC 2 cut(s) 697, 941
BsnI GGCC 3 cut(s) 274, 740, 1128
Bso31I GGTCTC 2 cut(s) 410, 849
BsoBI CYCGRG 2 cut(s) 241, 796
Bsp1286I GDGCHC 1 cut(s) 242
Bsp143I GATC 6 cut(s) 33, 415, 436, 751, 871, 1069
Bsp19I CCATGG 2 cut(s) 112, 1092
BspACI CCGC 6 cut(s) 17, 350, 426, 706, 820, 822
BspANI GGCC 3 cut(s) 274, 740, 1128
BspCNI CTCAG 1 cut(s) 727
BspFNI CGCG 2 cut(s) 708, 822
BspHI TCATGA 1 cut(s) 442
BspLI GGNNCC 4 cut(s) 21, 225, 246, 1090
BspPI GGATC 1 cut(s) 423
BspT107I GGYRCC 1 cut(s) 19
BspTNI GGTCTC 2 cut(s) 410, 849
BsrFI RCCGGY 2 cut(s) 69, 301
BssAI RCCGGY 2 cut(s) 69, 301
BssECI CCNNGG 6 cut(s) 112, 429, 660, 721, 820, 1092
BssMI GATC 6 cut(s) 33, 415, 436, 751, 871, 1069
BssT1I CCWWGG 3 cut(s) 112, 660, 1092
Bst2UI CCWGG 1 cut(s) 680
Bst4CI ACNGT 4 cut(s) 45, 141, 386, 965
Bst6I CTCTTC 3 cut(s) 257, 263, 558
BstC8I GCNNGC 1 cut(s) 646
BstDEI CTNAG 1 cut(s) 735
BstDSI CCRYGG 4 cut(s) 112, 429, 820, 1092
BstEII GGTNACC 1 cut(s) 674
BstF5I GGATG 3 cut(s) 634, 757, 1102
BstFNI CGCG 2 cut(s) 708, 822
BstHHI GCGC 3 cut(s) 11, 301, 710
BstKTI GATC 6 cut(s) 36, 418, 439, 754, 874, 1072
BstMAI GTCTC 3 cut(s) 410, 849, 902
BstMBI GATC 6 cut(s) 33, 415, 436, 751, 871, 1069
BstMCI CGRYCG 1 cut(s) 606
BstMWI GCNNNNNNNGC 3 cut(s) 176, 509, 1017
BstNI CCWGG 1 cut(s) 680
BstNSI RCATGY 1 cut(s) 203
BstPAI GACNNNNGTC 1 cut(s) 914
BstPI GGTNACC 1 cut(s) 674
BstSCI CCNGG 3 cut(s) 174, 247, 678
BstUI CGCG 2 cut(s) 708, 822
BstV1I GCAGC 2 cut(s) 512, 524
BsuRI GGCC 3 cut(s) 274, 740, 1128
BtgI CCRYGG 4 cut(s) 112, 429, 820, 1092
BtsCI GGATG 3 cut(s) 634, 757, 1102
BtsI GCAGTG 2 cut(s) 588, 893
BtsIMutI CAGTG 2 cut(s) 588, 893
Cac8I GCNNGC 1 cut(s) 646
CciI TCATGA 1 cut(s) 442
CfoI GCGC 3 cut(s) 11, 301, 710
Cfr10I RCCGGY 2 cut(s) 69, 301
Cfr13I GGNCC 4 cut(s) 116, 141, 245, 739
Cfr42I CCGCGG 1 cut(s) 823
CseI GACGC 1 cut(s) 530
CsiI ACCWGGT 1 cut(s) 678
Csp6I GTAC 4 cut(s) 20, 104, 421, 665
CviAII CATG 8 cut(s) 87, 113, 200, 409, 443, 485, 574, 1093
CviQI GTAC 4 cut(s) 20, 104, 421, 665
DdeI CTNAG 1 cut(s) 735
DpnI GATC 6 cut(s) 35, 417, 438, 753, 873, 1071
DpnII GATC 6 cut(s) 33, 415, 436, 751, 871, 1069
Eam1104I CTCTTC 3 cut(s) 257, 263, 558
EarI CTCTTC 3 cut(s) 257, 263, 558
EciI GGCGGA 1 cut(s) 415
Eco130I CCWWGG 3 cut(s) 112, 660, 1092
Eco24I GRGCYC 1 cut(s) 242
Eco31I GGTCTC 2 cut(s) 410, 849
Eco47I GGWCC 3 cut(s) 116, 141, 245
Eco88I CYCGRG 2 cut(s) 241, 796
Eco91I GGTNACC 1 cut(s) 674
EcoO109I RGGNCCY 1 cut(s) 739
EcoO65I GGTNACC 1 cut(s) 674
EcoRII CCWGG 1 cut(s) 678
EcoT14I CCWWGG 3 cut(s) 112, 660, 1092
EcoT38I GRGCYC 1 cut(s) 242
ErhI CCWWGG 3 cut(s) 112, 660, 1092
FaeI CATG 8 cut(s) 90, 116, 203, 412, 446, 488, 577, 1096
FalI AAGNNNNNCTT 2 cut(s) 831, 863
FaqI GGGAC 1 cut(s) 986
FatI CATG 8 cut(s) 86, 112, 199, 408, 442, 484, 573, 1092
FbaI TGATCA 1 cut(s) 751
Fnu4HI GCNGC 4 cut(s) 501, 513, 706, 820
FokI GGATG 3 cut(s) 641, 744, 1109
FriOI GRGCYC 1 cut(s) 242
Fsp4HI GCNGC 4 cut(s) 501, 513, 706, 820
FspBI CTAG 2 cut(s) 99, 762
GlaI GCGC 3 cut(s) 10, 300, 709
GluI GCNGC 4 cut(s) 501, 513, 706, 820
GsuI CTGGAG 1 cut(s) 1058
HaeIII GGCC 3 cut(s) 274, 740, 1128
HapII CCGG 4 cut(s) 70, 176, 248, 302
HgaI GACGC 1 cut(s) 530
HhaI GCGC 3 cut(s) 11, 301, 710
Hin1II CATG 8 cut(s) 90, 116, 203, 412, 446, 488, 577, 1096
Hin6I GCGC 3 cut(s) 9, 299, 708
HinP1I GCGC 3 cut(s) 9, 299, 708
HincII GTYRAC 2 cut(s) 319, 361
HindII GTYRAC 2 cut(s) 319, 361
HindIII AAGCTT 1 cut(s) 1061
HinfI GANTC 3 cut(s) 190, 320, 910
HpaII CCGG 4 cut(s) 70, 176, 248, 302
HphI GGTGA 3 cut(s) 33, 316, 710
Hpy166II GTNNAC 4 cut(s) 119, 319, 361, 382
Hpy188I TCNGA 6 cut(s) 33, 189, 195, 294, 751, 961
Hpy188III TCNNGA 4 cut(s) 443, 858, 932, 989
Hpy8I GTNNAC 4 cut(s) 119, 319, 361, 382
Hpy99I CGWCG 5 cut(s) 49, 136, 163, 232, 546
HpyAV CCTTC 5 cut(s) 90, 160, 214, 322, 562
HpyCH4III ACNGT 4 cut(s) 45, 141, 386, 965
HpyCH4IV ACGT 1 cut(s) 746
HpyCH4V TGCA 2 cut(s) 216, 512
HpyF10VI GCNNNNNNNGC 3 cut(s) 176, 509, 1017
HpyF3I CTNAG 1 cut(s) 735
HpySE526I ACGT 1 cut(s) 746
Hsp92II CATG 8 cut(s) 90, 116, 203, 412, 446, 488, 577, 1096
HspAI GCGC 3 cut(s) 9, 299, 708
KpnI GGTACC 1 cut(s) 23
Ksp22I TGATCA 1 cut(s) 751
KspI CCGCGG 1 cut(s) 823
Kzo9I GATC 6 cut(s) 33, 415, 436, 751, 871, 1069
LmnI GCTCC 2 cut(s) 223, 1039
Lsp1109I GCAGC 2 cut(s) 512, 524
MabI ACCWGGT 1 cut(s) 678
MaeI CTAG 2 cut(s) 99, 762
MaeII ACGT 1 cut(s) 746
MaeIII GTNAC 6 cut(s) 135, 209, 371, 652, 674, 979
MalI GATC 6 cut(s) 35, 417, 438, 753, 873, 1071
MboI GATC 6 cut(s) 33, 415, 436, 751, 871, 1069
MboII GAAGA 8 cut(s) 28, 75, 84, 274, 280, 377, 431, 575
MhlI GDGCHC 1 cut(s) 242
MluCI AATT 5 cut(s) 51, 506, 864, 1026, 1121
MlyI GAGTC 3 cut(s) 199, 314, 904
MmeI TCCRAC 4 cut(s) 149, 168, 317, 1003
MroXI GAANNNNTTC 1 cut(s) 170
MseI TTAA 1 cut(s) 947
MspA1I CMGCKG 2 cut(s) 503, 822
MspI CCGG 4 cut(s) 70, 176, 248, 302
MspR9I CCNGG 3 cut(s) 176, 249, 680
Mva1269I GAATGC 2 cut(s) 697, 941
MvaI CCWGG 1 cut(s) 680
MvnI CGCG 2 cut(s) 708, 822
MwoI GCNNNNNNNGC 3 cut(s) 176, 509, 1017
NciI CCSGG 2 cut(s) 176, 249
NcoI CCATGG 2 cut(s) 112, 1092
NdeII GATC 6 cut(s) 33, 415, 436, 751, 871, 1069
NlaIII CATG 8 cut(s) 90, 116, 203, 412, 446, 488, 577, 1096
NlaIV GGNNCC 4 cut(s) 21, 225, 246, 1090
NmeAIII GCCGAG 1 cut(s) 746
NmuCI GTSAC 2 cut(s) 135, 652
NspI RCATGY 1 cut(s) 203
PagI TCATGA 1 cut(s) 442
PcsI WCGNNNNNNNCGW 1 cut(s) 53
PctI GAATGC 2 cut(s) 697, 941
PdmI GAANNNNTTC 1 cut(s) 170
PkrI GCNGC 4 cut(s) 502, 514, 707, 821
PleI GAGTC 3 cut(s) 198, 314, 904
PpsI GAGTC 3 cut(s) 198, 314, 904
PshAI GACNNNNGTC 1 cut(s) 914
Psp6I CCWGG 1 cut(s) 678
PspEI GGTNACC 1 cut(s) 674
PspGI CCWGG 1 cut(s) 678
PspN4I GGNNCC 4 cut(s) 21, 225, 246, 1090
PspPI GGNCC 4 cut(s) 116, 141, 245, 739
PvuII CAGCTG 1 cut(s) 503
RsaI GTAC 4 cut(s) 21, 105, 422, 666
RsaNI GTAC 4 cut(s) 20, 104, 421, 665
SacII CCGCGG 1 cut(s) 823
SaqAI TTAA 1 cut(s) 947
SatI GCNGC 4 cut(s) 501, 513, 706, 820
Sau3AI GATC 6 cut(s) 33, 415, 436, 751, 871, 1069
Sau96I GGNCC 4 cut(s) 116, 141, 245, 739
ScaI AGTACT 1 cut(s) 105
SchI GAGTC 3 cut(s) 199, 314, 904
ScrFI CCNGG 3 cut(s) 176, 249, 680
SduI GDGCHC 1 cut(s) 242
SexAI ACCWGGT 1 cut(s) 678
Sfr303I CCGCGG 1 cut(s) 823
SgrAI CRCCGGYG 1 cut(s) 301
SgrBI CCGCGG 1 cut(s) 823
SinI GGWCC 3 cut(s) 116, 141, 245
SmlI CTYRAG 1 cut(s) 912
SmoI CTYRAG 1 cut(s) 912
Sse9I AATT 5 cut(s) 51, 506, 864, 1026, 1121
SsiI CCGC 6 cut(s) 17, 350, 426, 706, 820, 822
SspMI CTAG 2 cut(s) 99, 762
StyD4I CCNGG 3 cut(s) 174, 247, 678
StyI CCWWGG 3 cut(s) 112, 660, 1092
TaaI ACNGT 4 cut(s) 45, 141, 386, 965
TaiI ACGT 1 cut(s) 749
TaqI TCGA 3 cut(s) 47, 689, 874
TaqII GACCGA 1 cut(s) 620
TasI AATT 5 cut(s) 51, 506, 864, 1026, 1121
TatI WGTACW 1 cut(s) 103
TauI GCSGC 2 cut(s) 708, 822
Tru1I TTAA 1 cut(s) 947
Tru9I TTAA 1 cut(s) 947
TscAI CASTG 2 cut(s) 588, 893
TseFI GTSAC 2 cut(s) 135, 652
TseI GCWGC 2 cut(s) 500, 512
Tsp45I GTSAC 2 cut(s) 135, 652
TspDTI ATGAA 3 cut(s) 75, 431, 789
TspGWI ACGGA 1 cut(s) 45
TspRI CASTG 2 cut(s) 588, 893
VpaK11BI GGWCC 3 cut(s) 116, 141, 245
XapI RAATTY 2 cut(s) 51, 506
XceI RCATGY 1 cut(s) 203
XmnI GAANNNNTTC 1 cut(s) 170
XspI CTAG 2 cut(s) 99, 762
ZrmI AGTACT 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.