Rh1CG341700

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0007676
Physical Location & Seq
Forward (+)
61615991 .. 61619617
3627 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG341700.1

Sequence Viewer

Length: 855 bp
ATGCCGTGCGCTTTAGCGGTACCAAACTCTCCGATCTTCTCACCGTCGAAAATTCCGTCCATTTTCTGCCGGTCATCTCCTTCTTCATGTTCTTCGCCTAGAGTACTTCTCCATGGTCCACCTCCAACGCCGTCGTCACGGTCCAACAACCCACTTTCGTCGCCGAAGGCTTTCCCGGCAAGGTTTCAGAGTCAGAGGCATGTGATTAGTAACTGCAAGAAGGAGCCGTCGGGTTTGGGCTCGGGTCCGGGTTTGGCTTTGAAGAGGAAGAGGCCGATGAGGATTGATATTCCGGCTGCGCCGGTGAGTTTCGGCGTTGACTCGCCGAAGGTTGAGGAGAGAGTGGAGGCGGTGGAGGTTGACGAAGATGGATACTCGGTTTACTGTAAGAGAGGGAAAAGAGGTCTCATGGAGGATCGGTACTCCGCCGTGGTTGATCTTCATGATAATACCAGACAGGCGTTCTTTGGTGTGTTTGATGGGCATGGAGGTTCAAAGGCAGCTGAATTTGCAGCAAAGAACTTGGATAAGAACATCGTGGGTCGTTTGGCGAGTGGAGGAGAAGAGAAGGTCATGGAAGCAGTGAAAGATGGTTATTTGGCGACTGATGTTGAGTTTTTGAAAGAGGATGTTAGTGGTGGTGCTTGCTGTGTGACTGCGTTGGTACAGAAAGGTAATCTGGTGGTGTCGAATGCTGGTGATTGTCGCGCGGTTATGAGCCGAGGAGGGGTTGCTGAGGCCCTTACGTCTGATCATCATCCTTGTAGAACAGATGAAAGAGCCAGAATTGAAAGCTCGGTAAGCATTCAAATCTTGAAACTGAGTTGGACATTAATTTTCTCACATGGAAGATAA

Protein Analysis

284

Amino Acids

30.44

Weight (kDa)

8.84

Isoelectric Point (pI)

50.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 126 - 266 1.2e-26 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 19
AccB1I GGYRCC 1 cut(s) 19
AccII CGCG 2 cut(s) 708, 710
AciI CCGC 4 cut(s) 17, 350, 426, 710
AclWI GGATC 1 cut(s) 423
AcsI RAATTY 2 cut(s) 51, 506
AfaI GTAC 4 cut(s) 21, 105, 422, 666
AfiI CCNNNNNNNGG 1 cut(s) 727
AgsI TTSAA 6 cut(s) 262, 495, 622, 791, 809, 817
AluBI AGCT 2 cut(s) 503, 795
AluI AGCT 2 cut(s) 503, 795
Alw26I GTCTC 1 cut(s) 410
AlwI GGATC 1 cut(s) 423
Ama87I CYCGRG 1 cut(s) 241
AoxI GGCC 2 cut(s) 272, 738
ApeKI GCWGC 3 cut(s) 296, 500, 512
ApoI RAATTY 2 cut(s) 51, 506
ArsI GACNNNNNNTTYG 2 cut(s) 41, 73
AseI ATTAAT 1 cut(s) 833
Asp700I GAANNNNTTC 1 cut(s) 170
Asp718I GGTACC 1 cut(s) 19
AspLEI GCGC 3 cut(s) 11, 301, 710
AspS9I GGNCC 4 cut(s) 116, 141, 245, 739
AsuC2I CCSGG 2 cut(s) 176, 249
AsuHPI GGTGA 3 cut(s) 33, 316, 710
AvaI CYCGRG 1 cut(s) 241
AvaII GGWCC 3 cut(s) 116, 141, 245
BanI GGYRCC 1 cut(s) 19
BanII GRGCYC 1 cut(s) 242
BbvCI CCTCAGC 1 cut(s) 735
BbvI GCAGC 3 cut(s) 283, 512, 524
BccI CCATC 3 cut(s) 362, 473, 584
BceAI ACGGC 3 cut(s) 115, 211, 413
BciVI GTATCC 1 cut(s) 365
BclI TGATCA 1 cut(s) 751
BcnI CCSGG 2 cut(s) 176, 249
BcoDI GTCTC 1 cut(s) 410
BfaI CTAG 1 cut(s) 99
BfuI GTATCC 1 cut(s) 365
BisI GCNGC 3 cut(s) 297, 501, 513
BlsI GCNGC 3 cut(s) 298, 502, 514
BmcAI AGTACT 1 cut(s) 105
Bme1390I CCNGG 2 cut(s) 176, 249
Bme18I GGWCC 3 cut(s) 116, 141, 245
BmeT110I CYCGRG 1 cut(s) 241
BmgT120I GGNCC 4 cut(s) 116, 141, 245, 739
BmiI GGNNCC 3 cut(s) 21, 225, 246
BmrFI CCNGG 2 cut(s) 176, 249
BplI GAGNNNNNCTC 2 cut(s) 93, 125
Bpu10I CCTNAGC 1 cut(s) 735
BpuMI CCSGG 2 cut(s) 176, 249
BsaBI GATNNNNATC 1 cut(s) 756
BsaI GGTCTC 1 cut(s) 410
BsaJI CCNNGG 3 cut(s) 112, 429, 721
BsaXI ACNNNNNCTCC 2 cut(s) 404, 434
Bsc4I CCNNNNNNNGG 1 cut(s) 727
Bse118I RCCGGY 2 cut(s) 69, 301
Bse8I GATNNNNATC 1 cut(s) 756
BseDI CCNNGG 3 cut(s) 112, 429, 721
BseGI GGATG 2 cut(s) 634, 757
BseJI GATNNNNATC 1 cut(s) 756
BseLI CCNNNNNNNGG 1 cut(s) 727
BseMII CTCAG 2 cut(s) 726, 812
BseRI GAGGAG 3 cut(s) 350, 573, 738
BseXI GCAGC 3 cut(s) 283, 512, 524
Bsh1236I CGCG 2 cut(s) 708, 710
BshFI GGCC 2 cut(s) 274, 740
BshNI GGYRCC 1 cut(s) 19
BsiHKCI CYCGRG 1 cut(s) 241
BsiSI CCGG 5 cut(s) 70, 176, 248, 293, 302
BslI CCNNNNNNNGG 1 cut(s) 727
BsmAI GTCTC 1 cut(s) 410
BsmI GAATGC 2 cut(s) 697, 804
BsnI GGCC 2 cut(s) 274, 740
Bso31I GGTCTC 1 cut(s) 410
BsoBI CYCGRG 1 cut(s) 241
Bsp1286I GDGCHC 1 cut(s) 242
Bsp143I GATC 4 cut(s) 33, 415, 436, 751
Bsp19I CCATGG 1 cut(s) 112
BspACI CCGC 4 cut(s) 17, 350, 426, 710
BspANI GGCC 2 cut(s) 274, 740
BspCNI CTCAG 2 cut(s) 727, 813
BspFNI CGCG 2 cut(s) 708, 710
BspHI TCATGA 1 cut(s) 442
BspLI GGNNCC 3 cut(s) 21, 225, 246
BspPI GGATC 1 cut(s) 423
BspT107I GGYRCC 1 cut(s) 19
BspTNI GGTCTC 1 cut(s) 410
BsrFI RCCGGY 2 cut(s) 69, 301
BssAI RCCGGY 2 cut(s) 69, 301
BssECI CCNNGG 3 cut(s) 112, 429, 721
BssMI GATC 4 cut(s) 33, 415, 436, 751
BssT1I CCWWGG 1 cut(s) 112
Bst4CI ACNGT 3 cut(s) 45, 141, 386
Bst6I CTCTTC 3 cut(s) 257, 263, 558
BstC8I GCNNGC 1 cut(s) 646
BstDEI CTNAG 2 cut(s) 735, 821
BstDSI CCRYGG 2 cut(s) 112, 429
BstF5I GGATG 2 cut(s) 634, 757
BstFNI CGCG 2 cut(s) 708, 710
BstHHI GCGC 3 cut(s) 11, 301, 710
BstKTI GATC 4 cut(s) 36, 418, 439, 754
BstMAI GTCTC 1 cut(s) 410
BstMBI GATC 4 cut(s) 33, 415, 436, 751
BstMWI GCNNNNNNNGC 3 cut(s) 176, 509, 801
BstNSI RCATGY 1 cut(s) 203
BstSCI CCNGG 2 cut(s) 174, 247
BstUI CGCG 2 cut(s) 708, 710
BstV1I GCAGC 3 cut(s) 283, 512, 524
BsuI GTATCC 1 cut(s) 365
BsuRI GGCC 2 cut(s) 274, 740
BtgI CCRYGG 2 cut(s) 112, 429
BtsCI GGATG 2 cut(s) 634, 757
BtsI GCAGTG 1 cut(s) 588
BtsIMutI CAGTG 1 cut(s) 588
Cac8I GCNNGC 1 cut(s) 646
CciI TCATGA 1 cut(s) 442
CfoI GCGC 3 cut(s) 11, 301, 710
Cfr10I RCCGGY 2 cut(s) 69, 301
Cfr13I GGNCC 4 cut(s) 116, 141, 245, 739
Csp6I GTAC 4 cut(s) 20, 104, 421, 665
CviAII CATG 8 cut(s) 87, 113, 200, 409, 443, 485, 574, 845
CviQI GTAC 4 cut(s) 20, 104, 421, 665
DdeI CTNAG 2 cut(s) 735, 821
DpnI GATC 4 cut(s) 35, 417, 438, 753
DpnII GATC 4 cut(s) 33, 415, 436, 751
Eam1104I CTCTTC 3 cut(s) 257, 263, 558
EarI CTCTTC 3 cut(s) 257, 263, 558
EciI GGCGGA 1 cut(s) 415
Eco130I CCWWGG 1 cut(s) 112
Eco24I GRGCYC 1 cut(s) 242
Eco31I GGTCTC 1 cut(s) 410
Eco47I GGWCC 3 cut(s) 116, 141, 245
Eco88I CYCGRG 1 cut(s) 241
EcoO109I RGGNCCY 1 cut(s) 739
EcoT14I CCWWGG 1 cut(s) 112
EcoT38I GRGCYC 1 cut(s) 242
ErhI CCWWGG 1 cut(s) 112
FaeI CATG 8 cut(s) 90, 116, 203, 412, 446, 488, 577, 848
FaiI YATR 9 cut(s) 88, 114, 201, 410, 444, 486, 575, 716, 846
FatI CATG 8 cut(s) 86, 112, 199, 408, 442, 484, 573, 844
FbaI TGATCA 1 cut(s) 751
Fnu4HI GCNGC 3 cut(s) 297, 501, 513
FokI GGATG 2 cut(s) 641, 744
FriOI GRGCYC 1 cut(s) 242
Fsp4HI GCNGC 3 cut(s) 297, 501, 513
FspBI CTAG 1 cut(s) 99
GlaI GCGC 3 cut(s) 10, 300, 709
GluI GCNGC 3 cut(s) 297, 501, 513
HaeIII GGCC 2 cut(s) 274, 740
HapII CCGG 5 cut(s) 70, 176, 248, 293, 302
HhaI GCGC 3 cut(s) 11, 301, 710
Hin1II CATG 8 cut(s) 90, 116, 203, 412, 446, 488, 577, 848
Hin6I GCGC 3 cut(s) 9, 299, 708
HinP1I GCGC 3 cut(s) 9, 299, 708
HincII GTYRAC 2 cut(s) 319, 361
HindII GTYRAC 2 cut(s) 319, 361
HinfI GANTC 2 cut(s) 190, 320
HpaII CCGG 5 cut(s) 70, 176, 248, 293, 302
HphI GGTGA 3 cut(s) 33, 316, 710
Hpy166II GTNNAC 4 cut(s) 119, 319, 361, 382
Hpy188I TCNGA 4 cut(s) 33, 189, 195, 751
Hpy188III TCNNGA 2 cut(s) 443, 814
Hpy8I GTNNAC 4 cut(s) 119, 319, 361, 382
Hpy99I CGWCG 4 cut(s) 49, 136, 163, 232
HpyAV CCTTC 5 cut(s) 90, 160, 214, 322, 562
HpyCH4III ACNGT 3 cut(s) 45, 141, 386
HpyCH4IV ACGT 1 cut(s) 746
HpyCH4V TGCA 2 cut(s) 216, 512
HpyF10VI GCNNNNNNNGC 3 cut(s) 176, 509, 801
HpyF3I CTNAG 2 cut(s) 735, 821
HpySE526I ACGT 1 cut(s) 746
Hsp92II CATG 8 cut(s) 90, 116, 203, 412, 446, 488, 577, 848
HspAI GCGC 3 cut(s) 9, 299, 708
KpnI GGTACC 1 cut(s) 23
Ksp22I TGATCA 1 cut(s) 751
Kzo9I GATC 4 cut(s) 33, 415, 436, 751
LmnI GCTCC 1 cut(s) 223
Lsp1109I GCAGC 3 cut(s) 283, 512, 524
MaeI CTAG 1 cut(s) 99
MaeII ACGT 1 cut(s) 746
MaeIII GTNAC 3 cut(s) 135, 209, 652
MalI GATC 4 cut(s) 35, 417, 438, 753
MboI GATC 4 cut(s) 33, 415, 436, 751
MboII GAAGA 8 cut(s) 28, 75, 84, 274, 280, 377, 431, 575
MhlI GDGCHC 1 cut(s) 242
MluCI AATT 4 cut(s) 51, 506, 786, 834
MlyI GAGTC 2 cut(s) 199, 314
MmeI TCCRAC 3 cut(s) 149, 168, 806
MroXI GAANNNNTTC 1 cut(s) 170
MseI TTAA 1 cut(s) 833
MspA1I CMGCKG 1 cut(s) 503
MspI CCGG 5 cut(s) 70, 176, 248, 293, 302
MspR9I CCNGG 2 cut(s) 176, 249
Mva1269I GAATGC 2 cut(s) 697, 804
MvnI CGCG 2 cut(s) 708, 710
MwoI GCNNNNNNNGC 3 cut(s) 176, 509, 801
NciI CCSGG 2 cut(s) 176, 249
NcoI CCATGG 1 cut(s) 112
NdeII GATC 4 cut(s) 33, 415, 436, 751
NlaIII CATG 8 cut(s) 90, 116, 203, 412, 446, 488, 577, 848
NlaIV GGNNCC 3 cut(s) 21, 225, 246
NmeAIII GCCGAG 1 cut(s) 746
NmuCI GTSAC 2 cut(s) 135, 652
NspI RCATGY 1 cut(s) 203
PagI TCATGA 1 cut(s) 442
PcsI WCGNNNNNNNCGW 1 cut(s) 53
PctI GAATGC 2 cut(s) 697, 804
PdmI GAANNNNTTC 1 cut(s) 170
PkrI GCNGC 3 cut(s) 298, 502, 514
PleI GAGTC 2 cut(s) 198, 314
PpsI GAGTC 2 cut(s) 198, 314
PshBI ATTAAT 1 cut(s) 833
PspN4I GGNNCC 3 cut(s) 21, 225, 246
PspPI GGNCC 4 cut(s) 116, 141, 245, 739
PvuII CAGCTG 1 cut(s) 503
RsaI GTAC 4 cut(s) 21, 105, 422, 666
RsaNI GTAC 4 cut(s) 20, 104, 421, 665
SaqAI TTAA 1 cut(s) 833
SatI GCNGC 3 cut(s) 297, 501, 513
Sau3AI GATC 4 cut(s) 33, 415, 436, 751
Sau96I GGNCC 4 cut(s) 116, 141, 245, 739
ScaI AGTACT 1 cut(s) 105
SchI GAGTC 2 cut(s) 199, 314
ScrFI CCNGG 2 cut(s) 176, 249
SduI GDGCHC 1 cut(s) 242
SgrAI CRCCGGYG 1 cut(s) 301
SinI GGWCC 3 cut(s) 116, 141, 245
Sse9I AATT 4 cut(s) 51, 506, 786, 834
SsiI CCGC 4 cut(s) 17, 350, 426, 710
SspMI CTAG 1 cut(s) 99
StyD4I CCNGG 2 cut(s) 174, 247
StyI CCWWGG 1 cut(s) 112
TaaI ACNGT 3 cut(s) 45, 141, 386
TaiI ACGT 1 cut(s) 749
TaqI TCGA 2 cut(s) 47, 689
TasI AATT 4 cut(s) 51, 506, 786, 834
TatI WGTACW 1 cut(s) 103
Tru1I TTAA 1 cut(s) 833
Tru9I TTAA 1 cut(s) 833
TscAI CASTG 1 cut(s) 588
TseFI GTSAC 2 cut(s) 135, 652
TseI GCWGC 3 cut(s) 296, 500, 512
Tsp45I GTSAC 2 cut(s) 135, 652
TspDTI ATGAA 3 cut(s) 75, 431, 789
TspGWI ACGGA 1 cut(s) 45
TspRI CASTG 1 cut(s) 588
VpaK11BI GGWCC 3 cut(s) 116, 141, 245
VspI ATTAAT 1 cut(s) 833
XapI RAATTY 2 cut(s) 51, 506
XceI RCATGY 1 cut(s) 203
XmnI GAANNNNTTC 1 cut(s) 170
XspI CTAG 1 cut(s) 99
ZrmI AGTACT 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.